##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936374_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 57461 Sequences flagged as poor quality 0 Sequence length 101 %GC 31 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.3611667043734 31.0 31.0 34.0 28.0 34.0 2 30.29357303214354 31.0 31.0 34.0 27.0 34.0 3 30.26073336697934 31.0 31.0 34.0 26.0 34.0 4 33.76015036285481 37.0 35.0 37.0 30.0 37.0 5 33.83381771984477 37.0 35.0 37.0 30.0 37.0 6 34.11378152137972 37.0 35.0 37.0 31.0 37.0 7 34.06611440803327 37.0 35.0 37.0 30.0 37.0 8 34.12906145037504 37.0 35.0 37.0 31.0 37.0 9 35.48194427524756 39.0 37.0 39.0 30.0 39.0 10-11 35.53022919893493 39.0 37.0 39.0 30.0 39.0 12-13 35.38324254711892 39.0 37.0 39.0 30.0 39.0 14-15 36.006456553140396 40.0 36.0 41.0 27.5 41.0 16-17 36.04107133534049 39.0 36.5 41.0 28.5 41.0 18-19 36.206296444544996 39.5 35.5 41.0 30.5 41.0 20-21 36.248272741511634 40.0 35.0 41.0 31.0 41.0 22-23 36.016341518595226 39.5 35.0 41.0 30.5 41.0 24-25 35.83039800908442 39.0 35.0 41.0 30.0 41.0 26-27 35.401524512277895 38.5 35.0 41.0 29.5 41.0 28-29 34.915107638224185 38.0 35.0 40.5 27.5 41.0 30-31 34.13910304380362 36.0 35.0 40.0 26.0 41.0 32-33 33.182506395642264 35.0 34.0 39.5 22.0 41.0 34-35 32.50350672630132 35.0 33.0 39.0 9.0 41.0 36-37 31.926933050242774 35.0 33.0 39.0 2.0 41.0 38-39 31.38832425471189 35.0 33.0 38.5 2.0 41.0 40-41 30.712004664032996 35.0 33.0 38.0 2.0 41.0 42-43 29.9353996623797 35.0 32.5 38.0 2.0 41.0 44-45 28.996258331738048 35.0 30.0 38.0 2.0 41.0 46-47 27.859087032944082 35.0 22.0 38.0 2.0 41.0 48-49 26.862550251474914 35.0 2.0 38.0 2.0 41.0 50-51 26.04054054054054 35.0 2.0 37.5 2.0 40.5 52-53 25.604366439846157 35.0 2.0 37.0 2.0 40.0 54-55 25.199161170184993 35.0 2.0 37.0 2.0 41.0 56-57 24.635448391082647 35.0 2.0 37.0 2.0 41.0 58-59 24.083752458189032 35.0 2.0 36.5 2.0 41.0 60-61 23.518038321644248 34.0 2.0 36.0 2.0 40.0 62-63 22.946415829867213 33.0 2.0 35.0 2.0 40.0 64-65 22.450444649414386 33.0 2.0 35.0 2.0 40.0 66-67 21.93707906232053 33.0 2.0 35.0 2.0 39.0 68-69 21.421364055620334 32.5 2.0 35.0 2.0 39.0 70-71 20.88107586014862 31.5 2.0 35.0 2.0 38.0 72-73 20.332234036999008 30.5 2.0 35.0 2.0 37.0 74-75 19.86202815822906 29.5 2.0 35.0 2.0 37.0 76-77 19.385861714902283 28.5 2.0 35.0 2.0 36.0 78-79 18.959268025269314 26.5 2.0 35.0 2.0 35.0 80-81 18.5831520509563 25.5 2.0 35.0 2.0 35.0 82-83 18.194862602460802 22.0 2.0 35.0 2.0 35.0 84-85 17.809583891683054 17.5 2.0 35.0 2.0 35.0 86-87 17.43150136614399 4.5 2.0 35.0 2.0 35.0 88-89 17.001340039331026 2.0 2.0 35.0 2.0 35.0 90-91 16.524112006404344 2.0 2.0 34.0 2.0 35.0 92-93 16.10695950296723 2.0 2.0 34.0 2.0 35.0 94-95 15.702406849863387 2.0 2.0 34.0 2.0 35.0 96-97 15.257766137032075 2.0 2.0 34.0 2.0 35.0 98-99 14.847009275856667 2.0 2.0 33.5 2.0 35.0 100-101 13.723760463618802 2.0 2.0 31.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.15726926095823401 1101 2 -0.41192952416908923 1101 3 0.4052007236201902 1101 4 0.41264864254677036 1101 5 0.5030799933059669 1101 6 -0.10409858757984836 1101 7 -0.32726406940548713 1101 8 -0.48873632461020833 1101 9 -1.1903190304324198 1101 10-11 0.39400533345653344 1101 12-13 0.24342226537748246 1101 14-15 0.16576579899845711 1101 16-17 -0.0022847520050390813 1101 18-19 0.10580526480618602 1101 20-21 0.39751612280537785 1101 22-23 0.6787242846893946 1101 24-25 0.70449104572252 1101 26-27 0.4292016637709182 1101 28-29 -0.32250290489697875 1101 30-31 0.4930843687237214 1101 32-33 0.19804076044476204 1101 34-35 0.6560986454737261 1101 36-37 -0.9908896023510394 1101 38-39 -0.8274858746357374 1101 40-41 -0.1955085263967966 1101 42-43 -0.14505317693046038 1101 44-45 -0.7503130836804637 1101 46-47 -1.4545684652538533 1101 48-49 -1.0359005636540353 1101 50-51 -0.40375643315094933 1101 52-53 -1.0809844147260605 1101 54-55 -0.43574889666030003 1101 56-57 -0.05525226729142574 1101 58-59 0.7471829722591501 1101 60-61 1.6655151235513443 1101 62-63 2.1049414072719443 1101 64-65 1.5866699707811307 1101 66-67 1.2279735960141736 1101 68-69 1.3676198234232437 1101 70-71 1.3285000245344492 1101 72-73 0.8008756801609849 1101 74-75 1.0371871891485043 1101 76-77 0.10656870602069901 1101 78-79 0.45885279023345404 1101 80-81 0.42429280016620297 1101 82-83 1.0172861105288646 1101 84-85 1.5645317253221656 1101 86-87 0.5223190592549365 1101 88-89 -0.03908879309175717 1101 90-91 0.7270766249845977 1101 92-93 0.6272765395439333 1101 94-95 1.124103914501644 1101 96-97 0.9611990605266367 1101 98-99 1.2811806189913142 1101 100-101 1.0602315844329535 1102 1 0.6107862945973217 1102 2 0.7950149202753565 1102 3 0.8343673902868574 1102 4 0.5793153092134347 1102 5 1.0975244377504154 1102 6 1.240345856864593 1102 7 0.7241248194834071 1102 8 0.8390414531675745 1102 9 -0.17781903043242409 1102 10-11 1.073866444567642 1102 12-13 0.985783376488591 1102 14-15 1.1011824656651328 1102 16-17 0.6511874702171809 1102 18-19 1.5342774870284117 1102 20-21 1.552377233916495 1102 22-23 1.8231687291338403 1102 24-25 2.48574104572252 1102 26-27 2.5132294415487024 1102 28-29 2.590691539547464 1102 30-31 1.9889177020570585 1102 32-33 1.0424852048891964 1102 34-35 1.1449875343626204 1102 36-37 1.5223048420934049 1102 38-39 1.799597458697594 1102 40-41 2.3711581402698734 1102 42-43 3.104252378625091 1102 44-45 3.08927024965287 1102 46-47 3.455153756968368 1102 48-49 3.580766103012632 1102 50-51 3.693465789071272 1102 52-53 4.502348918607272 1102 54-55 4.2816122144508135 1102 56-57 4.671831066041907 1102 58-59 3.7805163055924815 1102 60-61 4.0314873457735665 1102 62-63 3.1979969628274993 1102 64-65 3.4929199707811307 1102 66-67 1.884918040458622 1102 68-69 1.3822031567565745 1102 70-71 1.6291944689788913 1102 72-73 1.0342090134943192 1102 74-75 0.11565941137072855 1102 76-77 -0.15315351620152384 1102 78-79 -0.3612860986554338 1102 80-81 -0.765984977611577 1102 82-83 -0.059102778360028196 1102 84-85 0.37078172532216414 1102 86-87 -0.5026809407450621 1102 88-89 -1.0863110153139814 1102 90-91 -1.4416733750153998 1102 92-93 -0.8018901271227357 1102 94-95 -0.6293683077205809 1102 96-97 -0.5033842728066968 1102 98-99 -0.4320138254531294 1102 100-101 -0.205740637789269 1103 1 0.8049529612639894 1103 2 0.9058482536086885 1103 3 0.4252007236201898 1103 4 0.5793153092134347 1103 5 0.9541911044170774 1103 6 0.7603458568645962 1103 7 0.9482914861500689 1103 8 0.9557081198342416 1103 9 1.0230143029009113 1103 10-11 1.391783111234318 1103 12-13 1.454533376488591 1103 14-15 1.779099132331801 1103 16-17 1.5066041368838512 1103 18-19 1.1680274870284109 1103 20-21 1.0019605672498173 1103 22-23 1.3131687291338423 1103 24-25 0.7578243790558545 1103 26-27 0.710312774882027 1103 28-29 0.8252748728807973 1103 30-31 0.6297510353903917 1103 32-33 1.259151871555865 1103 34-35 1.6083208676959444 1103 36-37 2.553554842093405 1103 38-39 2.915847458697595 1103 40-41 3.301158140269873 1103 42-43 4.11716904529176 1103 44-45 3.233020249652867 1103 46-47 2.6776537569683683 1103 48-49 3.280766103012631 1103 50-51 3.8317991224046075 1103 52-53 3.4156822519406056 1103 54-55 4.082028881117477 1103 56-57 2.8480810660419067 1103 58-59 3.06384963892582 1103 60-61 2.0977373457735666 1103 62-63 2.3404969628274976 1103 64-65 2.2433366374478005 1103 66-67 2.9290847071252877 1103 68-69 2.95761982342324 1103 70-71 3.6662778023122264 1103 72-73 4.047542346827651 1103 74-75 4.182742744704061 1103 76-77 4.034346483798476 1103 78-79 4.019963901344568 1103 80-81 3.536515022388425 1103 82-83 3.911730554973307 1103 84-85 3.8345317253221616 1103 86-87 4.3689857259216005 1103 88-89 3.9853556513526875 1103 90-91 3.963743291651266 1103 92-93 3.2139432062105993 1103 94-95 2.5885483589460865 1103 96-97 2.7578657271933036 1103 98-99 2.530069507880203 1103 100-101 2.5913426955440624 1104 1 0.9968678548810104 1104 2 0.0764865514810289 1104 3 0.9030730640457207 1104 4 1.4622940326176916 1104 5 1.3758932320766561 1104 6 1.1871543675028917 1104 7 1.1048872308309257 1104 8 1.1599634389831763 1104 9 2.0025887709860157 1104 10-11 1.7611448133619731 1104 12-13 1.0077248658502924 1104 14-15 1.565056579140311 1104 16-17 1.58447647730938 1104 18-19 1.2024955721348007 1104 20-21 1.3823860991647123 1104 22-23 1.695509154665757 1104 24-25 2.165483953523939 1104 26-27 2.3339297961586283 1104 28-29 2.2531472133063204 1104 30-31 2.174644652411665 1104 32-33 2.368726339640972 1104 34-35 2.063427250674671 1104 36-37 2.904618671880641 1104 38-39 2.93989001188908 1104 40-41 2.509456012610304 1104 42-43 2.4537647899726096 1104 44-45 2.5128074836954255 1104 46-47 2.3527601399470903 1104 48-49 1.8520426987573124 1104 50-51 2.1305225266599273 1104 52-53 2.117597145557628 1104 54-55 1.5952203704791792 1104 56-57 1.1206342575312682 1104 58-59 1.2095943197768797 1104 60-61 1.9749713883267574 1104 62-63 2.3456033458062215 1104 64-65 1.6682302544690764 1104 66-67 1.111425132657203 1104 68-69 1.3420879085296242 1104 70-71 1.2211714193335048 1104 72-73 0.7186061766148875 1104 74-75 0.19699806385299823 1104 76-77 0.09285712209635122 1104 78-79 0.02102773113180234 1104 80-81 -0.059867956334979766 1104 82-83 0.24896459752650202 1104 84-85 0.33687215085408084 1104 86-87 0.491113385496071 1104 88-89 -0.007410306094122632 1104 90-91 -0.17859713388065046 1104 92-93 -0.08690785761918995 1104 94-95 -0.4314516410539131 1104 96-97 0.006589131448622609 1104 98-99 0.5615588695823313 1104 100-101 0.5458107806504469 1105 1 -0.8095568426575781 1105 2 -0.29689684443052755 1105 3 -0.12028947245824284 1105 4 8.839366644153301E-4 1105 5 0.3671322808876667 1105 6 0.11289487647243845 1105 7 0.06358560379712941 1105 8 0.0890414531675745 1105 9 -0.0883582461186947 1105 10-11 -1.041942378961764 1105 12-13 -1.4670352509623896 1105 14-15 -1.5342342010015315 1105 16-17 -0.7820233140965342 1105 18-19 -1.0190313365009942 1105 20-21 -0.791372766083505 1105 22-23 -1.0297724473367467 1105 24-25 -0.4817834640813956 1105 26-27 -0.020471538843459314 1105 28-29 -0.29962708790351655 1105 30-31 0.1323000549982325 1105 32-33 -0.6143775402088423 1105 34-35 -1.5314830538726767 1105 36-37 -0.5444843735928693 1105 38-39 -1.4216035216945606 1105 40-41 -0.9523712714948331 1105 42-43 -2.067928993923921 1105 44-45 -2.5914895542686978 1105 46-47 -1.3499933018551609 1105 48-49 -0.3947240930658005 1105 50-51 -0.9560440148502956 1105 52-53 -0.5711804931574314 1105 54-55 -0.2030691580982058 1105 56-57 0.3133751836889651 1105 58-59 0.2020849330434622 1105 60-61 0.20734518891082132 1105 62-63 0.417359707925538 1105 64-65 0.8415719315654435 1105 66-67 1.190065099282151 1105 68-69 0.453894333227165 1105 70-71 0.45333662584164003 1105 72-73 0.8773462683962769 1105 74-75 0.959409411370725 1105 76-77 1.2059151112494568 1105 78-79 0.5817286072269212 1105 80-81 0.4974954145452877 1105 82-83 0.48133839811056234 1105 84-85 0.8566885880672608 1105 86-87 0.39682886317650556 1105 88-89 -0.9515070937453523 1105 90-91 -1.267041022074224 1105 92-93 -1.6903705192795986 1105 94-95 -2.0196869351715616 1105 96-97 -1.6486048610419903 1105 98-99 -1.1887540215315617 1105 100-101 -1.2835592652402479 1106 1 0.34730590244046056 1106 2 -0.0027791973717050666 1106 3 0.9777497432280313 1106 4 0.9028447209781376 1106 5 0.7004656142210024 1106 6 0.5442674254920448 1106 7 0.7498601136010521 1106 8 -0.008997762518703212 1106 9 -0.402083736314772 1106 10-11 0.8109987975088231 1106 12-13 1.1408078862925137 1106 14-15 0.6030207009592488 1106 16-17 1.1003296270799297 1106 18-19 1.3731255262441024 1106 20-21 0.9145095868576689 1106 22-23 0.5192471605063886 1106 24-25 1.1848832025852616 1106 26-27 1.4207049317447797 1106 28-29 1.3376278140572637 1106 30-31 0.9166137804884258 1106 32-33 1.5130734401833195 1106 34-35 0.3704777304410456 1106 36-37 1.0927705283679146 1106 38-39 0.4607494194819104 1106 40-41 0.6946875520345799 1106 42-43 0.4026592413701948 1106 44-45 0.4477261320058119 1106 46-47 0.002947874615426116 1106 48-49 0.7915504167381222 1106 50-51 0.19101480867911746 1106 52-53 -0.16921970884370552 1106 54-55 -0.036402491431541506 1106 56-57 0.6369045954536716 1106 58-59 0.9079672859846433 1106 60-61 0.7857765614598442 1106 62-63 0.005595002043182973 1106 64-65 -0.16823199000318212 1106 66-67 0.03320235418411244 1106 68-69 0.16958060773696815 1106 70-71 -0.05646729572698561 1106 72-73 0.053816856631573984 1106 74-75 -0.060198431766526284 1106 76-77 0.7059151112494568 1106 78-79 0.7483952738935855 1106 80-81 0.517103257682539 1106 82-83 0.7558482020321335 1106 84-85 1.2194336861064805 1106 86-87 1.3478092553333667 1106 88-89 1.4896693768428833 1106 90-91 1.9780570171414595 1106 92-93 2.5351196767988338 1106 94-95 2.1469797314951062 1106 96-97 2.292571609546245 1106 98-99 2.2916381353311834 1106 100-101 1.7654603426028874 1107 1 1.0899529612639896 1107 2 0.6283482536086886 1107 3 0.9593673902868574 1107 4 1.2251486425467704 1107 5 0.3475244377504154 1107 6 1.115345856864593 1107 7 1.0782914861500714 1107 8 1.2140414531675745 1107 9 1.384680969567576 1107 10-11 1.1467831112343134 1107 12-13 1.4337000431552624 1107 14-15 1.5282657989984614 1107 16-17 1.088687470217181 1107 18-19 1.4926108203617403 1107 20-21 1.7086272339164879 1107 22-23 1.0523353958005117 1107 24-25 0.9440743790558557 1107 26-27 1.315312774882031 1107 28-29 1.4761082062141213 1107 30-31 2.072251035390387 1107 32-33 2.125818538222532 1107 34-35 1.9054042010292775 1107 36-37 1.9181381754267406 1107 38-39 2.8100141253642583 1107 40-41 3.1940748069365377 1107 42-43 2.229252378625091 1107 44-45 1.5788535829862056 1107 46-47 1.6947370903017038 1107 48-49 0.5911827696792997 1107 50-51 -0.1086175442620565 1107 52-53 0.3773489186072716 1107 54-55 0.10452888111747782 1107 56-57 0.4426643993752428 1107 58-59 0.30134963892581723 1107 60-61 0.4064873457735665 1107 62-63 -1.1040863705058328 1107 64-65 -0.8820800292188657 1107 66-67 -1.1359152928747136 1107 68-69 -0.6490468432434255 1107 70-71 -0.2770555310211087 1107 72-73 -0.580374319839013 1107 74-75 -1.5510072552959393 1107 76-77 -0.9864868495348595 1107 78-79 -0.8300360986554338 1107 80-81 -0.6826516442782449 1107 82-83 -0.5903527783600282 1107 84-85 -0.40005160801117157 1107 86-87 -0.07559760741173349 1107 88-89 0.00743898468602211 1107 90-91 0.652076624984602 1107 92-93 -0.4789734604560696 1107 94-95 -0.9731183077205792 1107 96-97 -1.8158842728066968 1107 98-99 -1.1924304921197972 1107 100-101 -1.622407304455935 1108 1 -0.9239359276248997 1108 2 -0.9897073019468685 1108 3 -0.8762807578612914 1108 4 -0.9577217278236034 1108 5 -0.8006237103977369 1108 6 -1.2226171060983688 1108 7 -1.5605974027388143 1108 8 -1.188736324610204 1108 9 -1.1199486600620503 1108 10-11 -1.5615502220990152 1108 12-13 -1.5176888457336304 1108 14-15 -1.5897897565570887 1108 16-17 -1.1393217890420715 1108 18-19 -1.3071576981567787 1108 20-21 -1.6154468401575812 1108 22-23 -1.1675720116069002 1108 24-25 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6 -0.017229900711164703 2313 7 -0.17170851384992858 2313 8 -0.4867161225900034 2313 9 0.5248324847190915 2313 10-11 -0.4706411311899288 2313 12-13 -0.06819389623868233 2313 14-15 0.2839476171802815 2313 16-17 0.5744829247626413 2313 18-19 0.8174214264223494 2313 20-21 0.7995363248255813 2313 22-23 0.5958960018611137 2313 24-25 0.5908546820861602 2313 26-27 0.8494036839729375 2313 28-29 1.4173960850020038 2313 30-31 0.8203570959964495 2313 32-33 1.1182427806467743 2313 34-35 -0.021679132304051052 2313 36-37 0.41719120572976465 2313 38-39 -0.04415254130240598 2313 40-41 0.6438854129971467 2313 42-43 0.9498963180190358 2313 44-45 1.6375657041983231 2313 46-47 1.3509870903017038 2313 48-49 0.9368267090732374 2313 50-51 0.2427082133137013 2313 52-53 0.8659852822436385 2313 54-55 0.8374834265720246 2313 56-57 -0.08100984304899939 2313 58-59 -0.340695815619636 2313 60-61 -0.04711113907491793 2313 62-63 -0.512230309899774 2313 64-65 -0.39817851406735016 2313 66-67 -1.2609152928747136 2313 68-69 -1.936925631122211 2313 70-71 -1.837661591627164 2313 72-73 -1.7309425016571964 2313 74-75 -2.086045134083818 2313 76-77 -1.6095929101409183 2313 78-79 -1.3073088259281604 2313 80-81 -2.7640910382176376 2313 82-83 -2.227663384420632 2313 84-85 -1.9294076686172303 2313 86-87 -1.7261657892299134 2313 88-89 -2.2037352577382237 2313 90-91 -1.8138324659244915 2313 92-93 -1.6636325513651595 2313 94-95 -1.272360731963003 2313 96-97 -0.8888009394733647 2313 98-99 -0.9859910981804028 2313 100-101 -0.7710815468801773 2314 1 -1.2107227144116877 2314 2 -1.5692868815264482 2314 3 -1.232637114217649 2314 4 -1.8260900961919688 2314 5 -1.5804034901775168 2314 6 -1.2731676566489227 2314 7 -2.1581950003364128 2314 8 -2.0551026909765753 2314 9 -2.184012724126113 2314 10-11 -2.2484871590359603 2314 12-13 -2.5724936505384335 2314 14-15 -2.1198197865871222 2314 16-17 -1.4228553225756073 2314 18-19 -2.0881887291877987 2314 20-21 -1.7215529462636923 2314 22-23 -2.1160204600553527 2314 24-25 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2314 90-91 -1.9076080597000846 2314 92-93 -1.0952459829785912 2314 94-95 -0.9719921815944534 2314 96-97 -0.8437558944283179 2314 98-99 -0.8056061677954727 2314 100-101 -0.5286573044559351 2315 1 -0.588380372069345 2315 2 -0.39415174639131223 2315 3 -0.7147992763798108 2315 4 -0.5473513574532305 2315 5 -0.9591422289162566 2315 6 -0.6329874764687418 2315 7 -1.0517085138499311 2315 8 -0.8176252134990918 2315 9 -1.1103190304324215 2315 10-11 -0.7082168887656906 2315 12-13 -0.5121332901780775 2315 14-15 -0.6542342010015361 2315 16-17 -0.6667291964494808 2315 18-19 -1.485305846304918 2315 20-21 -1.618039432750173 2315 22-23 -1.756831270866158 2315 24-25 -1.8555089542774752 2315 26-27 -2.0063538917846344 2315 28-29 -2.0780584604525387 2315 30-31 -1.8202489646096112 2315 32-33 -1.9575147951107965 2315 34-35 -1.9883457989707196 2315 36-37 -3.1597784912399263 2315 38-39 -3.754152541302407 2315 40-41 -2.855508526396793 2315 42-43 -2.1961642880415724 2315 44-45 -2.490313083680462 2315 46-47 -2.205679576364968 2315 48-49 -1.922567230320702 2315 50-51 -1.7148675442620593 2315 52-53 -1.9276510813927281 2315 54-55 -1.8246377855491893 2315 56-57 -2.748585600624761 2315 58-59 -2.132817027740849 2315 60-61 -1.8589293208931004 2315 62-63 -1.5861697038391647 2315 64-65 -1.1866633625522027 2315 66-67 -1.5209152928747116 2315 68-69 -1.0190468432434265 2315 70-71 -0.9470555310211068 2315 72-73 -1.0991243198390173 2315 74-75 -0.7472572552959384 2315 76-77 -0.875653516201524 2315 78-79 -0.20336943198876867 2315 80-81 -1.5164427824387872E-4 2315 82-83 0.05506388830664122 2315 84-85 0.9911983919888314 2315 86-87 1.2223190592549358 2315 88-89 0.48535565135268754 2315 90-91 1.3537432916512664 2315 92-93 1.5606098728772668 2315 94-95 1.815215025612753 2315 96-97 1.4945323938599717 2315 98-99 1.7567361745468695 2315 100-101 2.044676028877399 2316 1 -0.32171370540267574 2316 2 -0.27415174639131124 2316 3 -0.4214659430464778 2316 4 -0.8540180241198954 2316 5 -0.3324755622495843 2316 6 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2316 68-69 0.5409531567565757 2316 70-71 0.8196111356455624 2316 72-73 0.4742090134943169 2316 74-75 0.6660760780373955 2316 76-77 0.3376798171318107 2316 78-79 0.8232972346778986 2316 80-81 1.3865150223884264 2316 82-83 1.1817305549733064 2316 84-85 1.5445317253221624 2316 86-87 0.44898572592160235 2316 88-89 0.8720223180193507 2316 90-91 0.9804099583179315 2316 92-93 0.5139432062106 2316 94-95 0.6752150256127525 2316 96-97 -0.01213427280669599 2316 98-99 1.3300695078802018 2316 100-101 1.8646760288773976 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2293.0 3 172.0 4 113.0 5 112.0 6 157.0 7 237.0 8 241.0 9 344.0 10 425.0 11 607.0 12 936.0 13 1195.0 14 1319.0 15 1617.0 16 2160.0 17 2645.0 18 2204.0 19 1478.0 20 1520.0 21 1452.0 22 1333.0 23 1169.0 24 1111.0 25 1066.0 26 975.0 27 1001.0 28 1030.0 29 1064.0 30 1179.0 31 1370.0 32 1504.0 33 1890.0 34 2669.0 35 7194.0 36 3833.0 37 3723.0 38 3357.0 39 766.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.75249421064545 21.550328208521233 14.381975519300749 9.315202061532569 2 15.381000452535943 29.39394994256275 37.433425000870265 17.79162460403105 3 23.38392453092909 38.96160406586138 28.245204859539808 9.409266543669718 4 10.7541291748612 24.151974520075882 33.94190438066728 31.151991924395634 5 6.8766861021669135 47.07162126881907 29.389957357932296 16.661735271081714 6 20.876118208082424 41.00038288837064 16.123081207142608 22.00041769640433 7 15.540587580061263 48.27694235588972 21.028265107212476 15.154204956836537 8 26.68523192063354 33.259072317465844 18.922635105734923 21.133060656165696 9 27.410708069344846 20.65724430829214 21.233377428113904 30.69867019424911 10-11 21.509250073965784 34.57595850954594 21.136810595381053 22.777980821107224 12-13 28.264350610923522 35.281268493055315 25.291537577888395 11.162843318132767 14-15 24.86707103758561 32.47295733219622 32.18577855905875 10.47419307115942 16-17 12.33159268929504 38.17580504786771 32.603133159268936 16.88946910356832 18-19 12.00915546891318 32.935319919236925 42.502436816820996 12.553087795028894 20-21 14.514079849629294 31.830206411639807 42.54759998607678 11.108113752654113 22-23 12.964929074928206 33.10155774084066 41.513358280393355 12.420154903837787 24-25 12.18189730200174 34.31679721496954 41.93733681462141 11.56396866840731 26-27 12.3669622577473 33.55814499917327 42.367571424841834 11.707321318237593 28-29 11.548604402203713 34.474355292131214 43.29442892329652 10.682611382368556 30-31 11.384385090032461 36.59956659095063 42.11639382783739 9.899654491179517 32-33 9.732218228654476 36.64006544422881 42.682343112256 10.945373214860714 34-35 9.562145660706497 36.49105974705626 42.1648495420846 11.781945050152638 36-37 10.722741052006256 36.59832091345104 41.79807280700988 10.880865227532826 38-39 11.91141783873504 34.78553332750939 41.71922774526077 11.583821088494801 40-41 10.354621085011185 35.878949944071586 41.877272091722595 11.88915687919463 42-43 11.190424184177187 36.3802367742781 41.29570573587873 11.133633305665983 44-45 11.314142897091722 36.23025027964206 40.39866470917226 12.05694211409396 46-47 10.672271845339019 36.40773304899579 39.499029872922094 13.420965232743102 48-49 11.894169165013242 36.06359639538847 38.27496088594429 13.767273553653997 50-51 12.319486695338998 36.30196860030071 37.361446204412744 14.01709849994755 52-53 11.729333822783925 35.73164123271628 37.345954166448166 15.193070778051638 54-55 11.873427013422818 35.41404502237137 36.94071588366891 15.771812080536913 56-57 12.496177136216282 35.42549566159573 36.0555036131524 16.02282358903559 58-59 11.848589703260984 34.51382335465381 36.30858760616546 17.32899933591975 60-61 11.968089754119847 33.90944200758436 35.894657742516124 18.22781049577967 62-63 12.541063814915777 33.19354162298176 35.94306982595932 18.32232473614315 64-65 12.48798392030062 32.701214716420516 36.24923534038277 18.561566022896095 66-67 12.741997483925077 32.16120352250489 36.14673609169695 18.95006290187308 68-69 13.273091141076844 32.10168928661942 35.45142317809608 19.17379639420766 70-71 13.494150641725714 31.786609818534473 34.95199070393248 19.767248835807333 72-73 14.023068857042992 30.360013981125483 35.03844809507166 20.578469066759876 74-75 15.068301593266852 29.75379963118014 35.27823176220733 19.899667013345685 76-77 14.828472064451862 29.112563569318954 36.06106149840094 19.997902867828245 78-79 15.199895136977323 28.948311268405647 36.403198322191635 19.448595272425393 80-81 15.456674473067917 29.764235030934323 35.52990317732182 19.249187318675943 82-83 15.442088889665659 29.71494485904539 34.643549993882935 20.199416257406018 84-85 15.771905910314215 28.999335919751147 34.701688161895774 20.527070008038866 86-87 16.537194487893114 28.706996740621644 34.15007121698023 20.605737554505023 88-89 17.656415231203518 27.811716175943644 34.00452706186694 20.527341530985904 90-91 18.27925029722358 27.696866913770197 33.3965661934401 20.62731659556612 92-93 18.50634593196042 28.374007901821614 32.59064368378728 20.529002482430684 94-95 19.134044040545266 28.076721426074798 31.6069556099266 21.18227892345334 96-97 19.097913827445225 27.460949785092776 31.275116189677465 22.166020197784533 98-99 20.402851053422314 26.60895845707697 30.84273784983054 22.14545263967017 100-101 21.861513462512885 26.64109866685304 29.27157409187007 22.225813778764 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3044.0 1 1743.0 2 764.5 3 979.5 4 566.0 5 395.0 6 778.5 7 1256.0 8 1622.0 9 1515.5 10 886.5 11 429.0 12 334.5 13 297.5 14 285.0 15 269.5 16 274.0 17 285.5 18 278.0 19 315.5 20 376.0 21 434.5 22 490.0 23 545.0 24 610.5 25 709.5 26 819.0 27 891.5 28 975.5 29 1079.5 30 1205.0 31 1340.0 32 1432.0 33 1470.5 34 1488.5 35 1531.5 36 1460.5 37 1302.5 38 1190.0 39 1064.5 40 1030.5 41 983.5 42 912.0 43 884.5 44 762.0 45 723.0 46 840.5 47 963.0 48 1791.0 49 2192.0 50 2293.5 51 2332.5 52 1569.5 53 1077.5 54 748.0 55 499.5 56 423.5 57 415.5 58 373.0 59 368.5 60 309.5 61 208.0 62 158.0 63 131.0 64 87.5 65 40.0 66 31.5 67 24.5 68 16.5 69 12.0 70 12.0 71 10.5 72 7.0 73 5.5 74 3.5 75 1.0 76 0.5 77 1.0 78 0.5 79 1.0 80 1.0 81 0.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04872870294634622 2 0.012182175736586556 3 0.012182175736586556 4 0.00696124327804946 5 0.01044186491707419 6 0.005220932458537095 7 0.008701554097561825 8 0.01044186491707419 9 0.015662797375611284 10-11 0.00348062163902473 12-13 0.012182175736586556 14-15 0.009571709507318008 16-17 0.019143419014636016 18-19 0.015662797375611284 20-21 0.005220932458537095 22-23 0.01044186491707419 24-25 0.019143419014636016 26-27 0.009571709507318008 28-29 0.021753885243904564 30-31 0.016532952785367466 32-33 0.013052331146342739 34-35 0.23668227145368162 36-37 0.395920711439063 38-39 0.3933102452097945 40-41 0.4263761507805294 42-43 0.40636257635613715 44-45 0.4263761507805294 46-47 0.43855832651711596 48-49 0.4463897252049216 50-51 0.45770174553175197 52-53 0.44203894815614064 54-55 0.4263761507805294 56-57 0.4168044412732114 58-59 0.4159342858634552 60-61 0.41419397504394284 62-63 0.40549242094638105 64-65 0.4281164616000418 66-67 0.39853117766833157 68-69 0.43072692782931027 70-71 0.40462226553662484 72-73 0.4194149075024799 74-75 0.43768817110735975 76-77 0.41767459668296764 78-79 0.4237656845512609 80-81 0.4228955291415047 82-83 0.424635839961017 84-85 0.4159342858634552 86-87 0.4202850629122361 88-89 0.43420754946833506 90-91 0.46118236717077665 92-93 0.4507405022537025 94-95 0.4194149075024799 96-97 0.3950505560293068 98-99 0.3811280694732079 100-101 0.3967908668488192 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 57461.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.64215728929187 #Duplication Level Percentage of deduplicated Percentage of total 1 83.41273821464394 34.73486364664729 2 7.167335339351387 5.969266110927411 3 3.121865596790371 3.90003654652721 4 1.6591440989635573 2.7636135813856355 5 1.007188231360749 2.0970745375123996 6 0.7438983617519225 1.8586519552392058 7 0.44299565362754934 1.2913106280781748 8 0.37612838515546637 1.2530237900489027 9 0.29254429956536276 1.09639581629279 >10 1.5086927449013707 11.459946746488923 >50 0.1420929455031762 4.2933467917370045 >100 0.09194249414911401 7.119611562625085 >500 0.012537612838515547 4.023598614712587 >1k 0.020896021397525912 18.13925967177738 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3454 6.011033570595709 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2493 4.338594873044325 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1900 3.306590557073493 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1449 2.5217103774734166 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1127 1.9613302935904353 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 853 1.4844851290440473 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 743 1.2930509388976872 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 716 1.2460625467708533 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 341 0.5934459894537164 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 293 0.5099110701171229 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 286 0.49772889438053636 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 273 0.4751048537268756 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 262 0.4559614347122396 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 257 0.4472598806146778 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 235 0.40897304258540573 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 212 0.3689458937366214 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 183 0.3184768799707628 No Hit GTATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 182 0.3167365691512504 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 181 0.31499625833173805 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 170 0.29585283931710205 No Hit GAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 160 0.2784497311219784 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 138 0.24016289309270636 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 135 0.23494196063416928 No Hit GGTATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 129 0.22450009571709506 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 118 0.20535667670245908 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 116 0.20187605506343434 No Hit TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 107 0.18621325768782304 No Hit GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 106 0.1844729468683107 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 105 0.18273263604879833 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 102 0.17751170359026122 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 100 0.1740310819512365 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 100 0.1740310819512365 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 98 0.17055046031221177 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 96 0.16706983867318703 No Hit GAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 94 0.16358921703416232 No Hit TATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 94 0.16358921703416232 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 92 0.16010859539513758 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 89 0.1548876629366005 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 88 0.15314735211708813 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 85 0.14792641965855102 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 81 0.14096517638050157 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 76 0.13226362228293972 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 75 0.13052331146342738 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 73 0.12704268982440264 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 72 0.12530237900489027 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 72 0.12530237900489027 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 69 0.12008144654635318 No Hit GTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 69 0.12008144654635318 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 69 0.12008144654635318 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 67 0.11660082490732844 No Hit GGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 66 0.1148605140878161 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 62 0.10789927080976662 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 62 0.10789927080976662 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 61 0.10615895999025425 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 60 0.10441864917074191 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 59 0.10267833835122954 No Hit ACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 58 0.10093802753171717 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 58 0.10093802753171717 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.004350777048780913 0.0 18-19 0.0 0.0 0.0 0.01044186491707419 0.0 20-21 0.0 0.0 0.0 0.02697481770244166 0.0 22-23 0.0 0.0 0.0 0.05307947999512713 0.0 24-25 0.0 0.0 0.0 0.08614538556586207 0.0 26-27 0.0 0.0 0.0 0.1400950209707454 0.0 28-29 0.0 0.0 0.0 0.27844973112197835 0.0 30-31 0.0 0.0 0.0 0.5133916917561476 0.0 32-33 0.0 0.0 0.0 0.8710255651659387 0.0 34-35 0.0 0.0 0.0 1.3687544595464751 0.0 36-37 0.0 0.0 0.0 2.0248516385026365 0.0 38-39 0.0 0.0 0.0 2.7966794869563705 0.0 40-41 0.0 0.0 0.0 3.6268077478637686 0.0 42-43 0.0 0.0 0.0 4.2541897982979755 0.0 44-45 0.0 0.0 0.0 4.767581490054123 0.0 46-47 0.0 0.0 0.0 5.413236804093211 0.0 48-49 0.0 0.0 0.0 6.184194497137189 0.0 50-51 0.0 0.0 0.0 6.766328466264074 0.0 52-53 0.0 0.0 0.0 7.184003062947042 0.0 54-55 0.0 0.0 0.0 7.546857868815371 0.0 56-57 0.0 0.0 0.0 7.848801796000766 0.0 58-59 0.0 0.0 0.0 8.113329040566645 0.0 60-61 0.0 0.0 0.0 8.476183846434974 0.0 62-63 0.0 0.0 0.0 8.765945597883782 0.0 64-65 0.0 0.0 0.0 9.022641443761856 0.0 66-67 0.0 0.0 0.0 9.31327335062042 0.0 68-69 0.0 0.0 0.0 9.606515723708254 0.0 70-71 0.0 0.0 0.0 9.911940272532675 0.0 72-73 0.0 0.0 0.0 10.235638084961973 0.0 74-75 0.0 0.0 0.0 10.519308748542489 0.0 76-77 0.0 0.0 0.0 10.795148013435199 0.0 78-79 0.0 0.0 0.0 11.10057256225962 0.0 80-81 0.0 0.0 0.0 11.412088198952333 0.0 82-83 0.0 0.0 0.0 11.754929430396269 0.0 84-85 0.0 0.0 0.0 12.136927655279234 0.0 86-87 0.0 0.0 0.0 12.470197177215852 0.0 88-89 0.0 0.0 0.0 12.756478307025635 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATAA 20 0.0020994532 70.9449 2 ACATAAG 20 0.0020994532 70.9449 3 CATAAGC 20 0.0020994532 70.9449 4 GTACATA 35 3.462952E-6 67.56658 1 CTGTCTA 25 0.0050826683 56.755924 9 AGTAAAA 195 0.0 31.531069 2 GAGTAAA 200 0.0 30.742794 1 CCACCAG 35 0.008286384 27.192438 88-89 CAGAGAA 90 6.872142E-9 26.27589 10-11 GTATCAA 2535 0.0 25.56069 1 AACGCAG 2695 0.0 24.21867 6 TATCAAC 2685 0.0 24.132717 2 TCAACGC 2760 0.0 23.476938 4 ATCAACG 2760 0.0 23.476938 3 CAACGCA 2775 0.0 23.350035 5 GTAAAAA 265 3.092282E-11 23.202106 3 AGTACTC 235 4.645699E-9 22.138836 2 TGGGGAA 150 3.068999E-5 22.071749 6 ACGCAGA 2950 0.0 21.804535 7 CGCAGAG 2970 0.0 21.498457 8 >>END_MODULE