FastQCFastQC Report
Thu 26 May 2016
SRR936374_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936374_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57461
Sequences flagged as poor quality0
Sequence length101
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36406.334731383025008No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24284.225474669776022No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19493.391865787229599No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14672.5530359722246394No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10911.89867910408799No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9401.635892170341623No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7291.2686865874245141No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7141.2425819251318284No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT5510.958911261551313No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3200.5568994622439568No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA3130.5447172865073702No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2670.46466298880980145No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2610.45422112389272723No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2560.44551956979516544No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT2560.44551956979516544RNA PCR Primer, Index 33 (95% over 22bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA2410.4194149075024799No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2170.3776474478341832No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2050.3567637180000348No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2050.3567637180000348No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG2040.35502340718052244RNA PCR Primer, Index 33 (96% over 29bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.35502340718052244No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT1750.30455439341466384No Hit
GTATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1670.29063190685856494No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT1620.28193035276100314RNA PCR Primer, Index 33 (95% over 24bp)
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG1500.26104662292685477No Hit
GGTATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1420.24712413637075584No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1310.2279807173561198No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.22101947407807032No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1260.21927916325855798No Hit
GAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.21753885243904564No Hit
TATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.21057760916099616No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1120.1949148117853849No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT1060.1844729468683107No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.1844729468683107No Hit
TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1050.18273263604879833No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1040.18099232522928596No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1030.1792520144097736No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA990.17229077113172414No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG960.16706983867318703No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC940.16358921703416232No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC860.1496667304780634No Hit
GAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.14270548720001391No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT820.14270548720001391No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA780.13574424392196446RNA PCR Primer, Index 33 (96% over 29bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.13574424392196446No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.1340039331024521No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.13052331146342738No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT730.12704268982440264No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG730.12704268982440264No Hit
GAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.12530237900489027No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC710.12356206818537792No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT710.12356206818537792No Hit
GGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.12182175736586555No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA690.12008144654635318No Hit
GTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.11834113572684081No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT680.11834113572684081RNA PCR Primer, Index 33 (96% over 27bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.11312020326830373No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG640.11137989244879136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC640.11137989244879136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG620.10789927080976662No Hit
TATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.10615895999025425No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG600.10441864917074191No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG600.10441864917074191No Hit
GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.10093802753171717No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG580.10093802753171717No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA580.10093802753171717No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTGA250.004948387457.140961
GTATATG250.004948387457.140961
TATATGG250.00499997256.9916422
ATGGGAC250.005017255456.942045
ATATGGG250.005017255456.942043
TGGGCAG459.596728E-442.1792876
ATGGGAT608.242009E-539.5430835
ATGGGCA500.001611100337.961365
CGCAGAA756.855975E-637.9613579
ATGGGGG702.0414001E-433.894075
CATGGGC2950.027.3450454
GAGTACC350.00840231827.11525712-13
CATGGGG2303.6379788E-1226.8205244
AGTACTC1858.689312E-925.6495675
GTACAAA1501.0090098E-625.3959831
GGTATCA19450.025.2164461
GTATCAA26000.023.8087331
ATGGGGA1452.3028577E-522.9077175
TACAAAA1703.0674073E-622.349662
AGTAAAA1700.022.3302112-13
Produced by FastQC (version 0.11.5)