FastQCFastQC Report
Thu 26 May 2016
SRR936372_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936372_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335865
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24280.7229095023298051No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20280.6038140324237417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14490.4314233397347148No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9840.2929748559689161No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9720.28940199187173415No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3780.11254521906122997No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA3480.10361305881827519No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3400.10123114942015395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC353.4759323E-667.644453
TAACGCA452.0881089E-763.134814
TACCTGG4600.054.5485882
GTACCTG5050.050.6404151
GTATCAA15000.043.569511
TATAACG555.0528775E-543.040052
GTACACG901.2241799E-842.0961461
GGTATCA7700.041.2077521
GTACATG18850.033.916991
ATCAACG19250.033.6992343
TACATGG19050.033.302382
TATCAAC19550.033.1771622
ACATGGG18850.032.4047363
CAACGCA20200.031.8799575
TCAACGC20250.031.801244
ACCTGGG7600.031.7750873
ACACGGG1051.9434083E-631.5674083
AACGCAG20750.031.4913436
GTGTATC1252.2032145E-730.3092271
CATGGGT3000.028.4106664