##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936372_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 335865 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.595477349530317 33.0 31.0 34.0 30.0 34.0 2 31.635785211320023 33.0 31.0 34.0 30.0 34.0 3 31.706935822428655 34.0 31.0 34.0 30.0 34.0 4 35.21164753695681 37.0 35.0 37.0 33.0 37.0 5 35.22922602831495 37.0 35.0 37.0 33.0 37.0 6 35.32731603471633 37.0 35.0 37.0 33.0 37.0 7 35.2891935747994 37.0 35.0 37.0 33.0 37.0 8 35.28413797210189 37.0 35.0 37.0 33.0 37.0 9 36.938999300314116 39.0 37.0 39.0 33.0 39.0 10-11 36.84200050615575 39.0 37.0 39.0 33.0 39.0 12-13 36.74467419945513 39.0 37.0 39.0 33.0 39.0 14-15 37.93592663719053 40.0 38.0 41.0 33.0 41.0 16-17 37.885876765962514 40.0 38.0 41.0 33.0 41.0 18-19 37.8823173001057 40.0 38.0 41.0 33.0 41.0 20-21 37.77179223795275 40.0 38.0 41.0 33.0 41.0 22-23 37.54215384157325 40.0 37.5 41.0 32.0 41.0 24-25 37.64889911125006 40.0 38.0 41.0 32.5 41.0 26-27 37.55513822517976 40.0 37.5 41.0 32.0 41.0 28-29 37.394281928751134 40.0 37.0 41.0 32.0 41.0 30-31 37.13998928140771 40.0 37.0 41.0 31.5 41.0 32-33 36.81014395664925 40.0 36.0 41.0 31.0 41.0 34-35 36.41350096020723 40.0 35.5 41.0 30.0 41.0 36-37 36.12963988507287 40.0 35.0 41.0 29.5 41.0 38-39 35.94177124737618 40.0 35.0 41.0 28.5 41.0 40-41 35.73438881693538 39.5 35.0 41.0 27.5 41.0 42-43 35.506041117710986 39.0 35.0 41.0 26.5 41.0 44-45 35.252664016792465 39.0 35.0 41.0 25.5 41.0 46-47 34.880136364313046 39.0 35.0 41.0 23.5 41.0 48-49 34.61652449644947 39.0 35.0 41.0 22.0 41.0 50-51 34.273181188870524 38.5 34.5 40.5 20.5 41.0 52-53 34.27652330549477 39.0 34.5 40.0 20.0 41.0 54-55 34.34221190061483 39.0 35.0 41.0 20.0 41.0 56-57 34.18119929138196 39.0 34.5 41.0 18.5 41.0 58-59 33.95282181829009 38.0 34.0 41.0 17.5 41.0 60-61 33.67696098134667 38.0 34.0 40.0 14.0 41.0 62-63 33.38383725604037 37.0 34.0 40.0 12.0 41.0 64-65 33.065533771009186 37.0 33.0 40.0 8.0 41.0 66-67 32.72606702097569 36.0 33.0 39.5 7.0 41.0 68-69 32.35443705060069 36.0 33.0 39.0 2.0 41.0 70-71 31.958443124469653 35.0 32.5 39.0 2.0 41.0 72-73 31.56959790391973 35.0 32.0 38.0 2.0 40.0 74-75 31.173467017998302 35.0 32.0 37.0 2.0 39.5 76-77 30.790478317181012 35.0 31.0 37.0 2.0 39.0 78-79 30.4054828576958 35.0 31.0 36.0 2.0 39.0 80-81 30.020150953508107 35.0 31.0 36.0 2.0 37.5 82-83 29.66240007145728 35.0 30.5 35.5 2.0 37.0 84-85 29.339882095484793 34.5 30.0 35.0 2.0 37.0 86-87 29.025504294880385 34.0 30.0 35.0 2.0 36.0 88-89 28.69926011939321 34.0 29.5 35.0 2.0 36.0 90-91 28.344366039926754 34.0 29.0 35.0 2.0 36.0 92-93 28.04061304393134 34.0 29.0 35.0 2.0 35.0 94-95 27.670568531999464 34.0 28.5 35.0 2.0 35.0 96-97 27.245451297396276 34.0 27.0 35.0 2.0 35.0 98-99 26.77200214371846 34.0 26.5 35.0 2.0 35.0 100-101 23.7448186027124 30.0 13.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2729261459377845 1101 2 0.1819190897254046 1101 3 0.14043753775240475 1101 4 0.08996207094806152 1101 5 0.293027217510641 1101 6 0.25372156336629814 1101 7 0.007650734895030098 1101 8 0.35627942517306366 1101 9 0.21267105318271717 1101 10-11 0.3443818711470712 1101 12-13 0.31689604631826285 1101 14-15 0.35658019455641465 1101 16-17 0.2689205718277705 1101 18-19 -1.1097855773556375E-4 1101 20-21 0.15360015571469177 1101 22-23 0.10102582154986095 1101 24-25 0.14407925731800475 1101 26-27 0.20946020850064428 1101 28-29 0.1413731173750321 1101 30-31 0.12494835840841034 1101 32-33 -0.004646018430470633 1101 34-35 -0.45880423260484093 1101 36-37 -0.2084335540253477 1101 38-39 0.17283835908131095 1101 40-41 -0.2649888100096618 1101 42-43 -0.35355727231769407 1101 44-45 -0.24120158339162145 1101 46-47 -0.43721216835411525 1101 48-49 -0.13814667398980873 1101 50-51 -0.09097029463484319 1101 52-53 -0.5719818420132583 1101 54-55 -0.6794749620432654 1101 56-57 -0.756089478152802 1101 58-59 -0.7034404155975054 1101 60-61 -1.0193715977067157 1101 62-63 -0.835189618848041 1101 64-65 -1.1112840414363916 1101 66-67 -1.010923808879145 1101 68-69 -0.9601822821691144 1101 70-71 -0.7482884939639689 1101 72-73 -0.7763594868153803 1101 74-75 -0.9675817567832468 1101 76-77 -0.6855823893593893 1101 78-79 -0.557347676577308 1101 80-81 -0.7115923544570819 1101 82-83 -0.6844137357882225 1101 84-85 -0.9461144633358103 1101 86-87 -0.7943798237309316 1101 88-89 -0.7330659693795276 1101 90-91 -1.0790678324695513 1101 92-93 -0.9611986033743598 1101 94-95 -0.4636230162445365 1101 96-97 -0.5837542886453129 1101 98-99 -0.20097765991885908 1101 100-101 -0.0033430328995471825 1102 1 0.12382408068786077 1102 2 0.3526763480313022 1102 3 0.31070093194575676 1102 4 0.3051072370659895 1102 5 0.4747093486091103 1102 6 0.22699303897240242 1102 7 0.19202667024372744 1102 8 0.3867493443589396 1102 9 0.3370350160680786 1102 10-11 0.7162615478905607 1102 12-13 0.5458918559560999 1102 14-15 0.5347154833920911 1102 16-17 0.6293515805078087 1102 18-19 0.9192544808855416 1102 20-21 0.741814762119958 1102 22-23 0.819283828134715 1102 24-25 0.9739580361267457 1102 26-27 0.38790977449884423 1102 28-29 0.6510483643070799 1102 30-31 0.8455035813955405 1102 32-33 0.3695084263465418 1102 34-35 0.1652978326450878 1102 36-37 0.17571041484624317 1102 38-39 -0.3144034846780457 1102 40-41 -0.21186100396356977 1102 42-43 -0.5101645755203279 1102 44-45 -0.25786575579509474 1102 46-47 -0.3630652063666844 1102 48-49 -0.34526280688415056 1102 50-51 -0.35643722070487627 1102 52-53 -0.39932395515304364 1102 54-55 -0.26779432750270615 1102 56-57 0.12572734315818934 1102 58-59 -0.12190794029070418 1102 60-61 0.04970951573236704 1102 62-63 0.08152693308252168 1102 64-65 -0.2170832033639556 1102 66-67 0.18049343146805086 1102 68-69 -0.34929482332446327 1102 70-71 -0.17379732365568046 1102 72-73 -0.2856156975307371 1102 74-75 -0.2644464679475682 1102 76-77 -0.49471887544140003 1102 78-79 -0.22304656912444898 1102 80-81 -0.3867719414070976 1102 82-83 0.3214153572976848 1102 84-85 0.2009747315160304 1102 86-87 -0.31282490005538577 1102 88-89 -0.10802855543160561 1102 90-91 0.1406343525050069 1102 92-93 0.24145030413836466 1102 94-95 -0.029718496639624448 1102 96-97 -0.49926611145285804 1102 98-99 -0.3058190247796837 1102 100-101 -0.32167586738023957 1103 1 0.24360430046807835 1103 2 0.03207195242690375 1103 3 0.20493170117653037 1103 4 0.07159075354950772 1103 5 0.23047857937834237 1103 6 0.2753446873240506 1103 7 0.3535651317821902 1103 8 0.3743867069963045 1103 9 0.18373831277137498 1103 10-11 0.19359671272572143 1103 12-13 0.3435566911209378 1103 14-15 0.27770998888659193 1103 16-17 0.27234608600231525 1103 18-19 0.13477645890751688 1103 20-21 -0.12315776535256617 1103 22-23 0.04826734461823179 1103 24-25 0.18714484931356168 1103 26-27 0.29354164263072136 1103 28-29 0.27096594672466523 1103 30-31 0.44866292205488634 1103 32-33 0.4405249098630222 1103 34-35 -0.02948238713513973 1103 36-37 0.05785327198910295 1103 38-39 0.03985475708019948 1103 40-41 -0.40403133363389543 1103 42-43 -0.17142831178406936 1103 44-45 -0.25209652502586977 1103 46-47 0.12635787055639724 1103 48-49 0.3897646656433196 1103 50-51 0.41141992215226253 1103 52-53 0.12443978111070209 1103 54-55 0.4120133648049915 1103 56-57 0.3126778926087326 1103 58-59 0.6287788728961061 1103 60-61 0.6253963289191873 1103 62-63 0.7397686913242794 1103 64-65 0.4480266867459335 1103 66-67 0.18214178311640694 1103 68-69 0.5191117700821337 1103 70-71 0.38677959942124573 1103 72-73 0.02619748928245258 1103 74-75 0.07676232326122445 1103 76-77 -0.013125468847992039 1103 78-79 -0.00958503066291172 1103 80-81 -0.19679941393457057 1103 82-83 -0.05124947786715239 1103 84-85 -0.37278900474770893 1103 86-87 -0.22450072423120915 1103 88-89 0.08565276324971194 1103 90-91 -0.10606894419828805 1103 92-93 -0.15484090465284694 1103 94-95 -0.156778936200066 1103 96-97 0.17367344898670467 1103 98-99 0.15681833785768262 1103 100-101 0.11733512163074877 1104 1 -0.03252810371310488 1104 2 0.1330301405802139 1104 3 0.20902577783158094 1104 4 0.09476148525682504 1104 5 -0.019260096579841957 1104 6 0.09172099394425715 1104 7 0.17272889484839027 1104 8 0.039979041491072564 1104 9 0.179034480018764 1104 10-11 0.314371974049763 1104 12-13 0.1614573879850454 1104 14-15 0.32226573801551694 1104 16-17 0.4662049710197351 1104 18-19 0.04117889793190699 1104 20-21 0.43359310572756726 1104 22-23 0.4267081111687574 1104 24-25 0.3833992047142587 1104 26-27 0.18844582381538544 1104 28-29 -0.04275356547045561 1104 30-31 0.13868905445907131 1104 32-33 0.15319912589090023 1104 34-35 0.03480332715057699 1104 36-37 0.12701703505530304 1104 38-39 -0.054788100062658884 1104 40-41 0.3626498510350942 1104 42-43 0.7573173328152336 1104 44-45 0.4492972031971334 1104 46-47 0.501052992507617 1104 48-49 0.8879789513576029 1104 50-51 0.530235253162715 1104 52-53 0.6803195720514665 1104 54-55 0.9524750372788588 1104 56-57 1.0286622131662284 1104 58-59 1.3574809634884417 1104 60-61 1.2510496390237122 1104 62-63 1.2779220014288057 1104 64-65 1.0487235508574315 1104 66-67 1.1256086820711104 1104 68-69 1.3056971359357945 1104 70-71 0.9857778572609632 1104 72-73 0.8540720537423852 1104 74-75 0.8169191176863144 1104 76-77 1.0330417785387631 1104 78-79 0.7568783839712374 1104 80-81 0.5742371714312853 1104 82-83 0.8561982573244826 1104 84-85 0.5310001938585671 1104 86-87 0.28765084371304184 1104 88-89 0.5111754113333369 1104 90-91 0.6934955157320246 1104 92-93 0.8258646702600494 1104 94-95 0.306940575995057 1104 96-97 0.3018528914954146 1104 98-99 0.6628287908193506 1104 100-101 1.0361504526411984 1105 1 0.48118194021963134 1105 2 0.361037552044678 1105 3 0.35367752057452506 1105 4 0.21079285579508422 1105 5 0.2038063385756672 1105 6 0.33635758746738276 1105 7 0.3692841953273387 1105 8 0.26567911024522317 1105 9 0.3888744809510243 1105 10-11 0.49034181544908506 1105 12-13 0.5578483776952226 1105 14-15 0.3546151489439282 1105 16-17 0.4477462293372412 1105 18-19 0.5399902668387213 1105 20-21 0.3311960330229766 1105 22-23 0.5423607512116391 1105 24-25 0.2519680695715607 1105 26-27 0.15446495844533814 1105 28-29 0.5668510398923701 1105 30-31 0.5909049191881834 1105 32-33 0.1305452156441973 1105 34-35 0.21922759853136853 1105 36-37 0.17880406033119556 1105 38-39 0.07731892334870594 1105 40-41 -0.07975398055219785 1105 42-43 0.040922381001408326 1105 44-45 0.01579645156276399 1105 46-47 0.18952676687746361 1105 48-49 -0.1144099640747882 1105 50-51 0.31270993648575995 1105 52-53 0.27408741608442 1105 54-55 0.21690466915281803 1105 56-57 0.13124573780701354 1105 58-59 -0.04364707072549123 1105 60-61 0.17278643880929678 1105 62-63 -0.13502825086396797 1105 64-65 0.03383652907751866 1105 66-67 0.016697444845981124 1105 68-69 0.1589827686487837 1105 70-71 0.003042141227261652 1105 72-73 0.14941774728531954 1105 74-75 0.12075420094875255 1105 76-77 0.3001807901104421 1105 78-79 0.330130688400633 1105 80-81 0.28622136962969336 1105 82-83 0.28955917000671505 1105 84-85 0.33283091880700155 1105 86-87 0.45766004977738817 1105 88-89 0.5098644211570225 1105 90-91 0.34807582407691484 1105 92-93 0.5175372606601023 1105 94-95 0.46585006523328687 1105 96-97 0.5651987714902873 1105 98-99 0.775201042109952 1105 100-101 1.0326719587067146 1106 1 0.4145055468726788 1106 2 0.31757051426775007 1106 3 0.39436123137786794 1106 4 0.17211807857347594 1106 5 0.010847706895120268 1106 6 0.09049329710353504 1106 7 0.08645103782245656 1106 8 0.2072965823558377 1106 9 0.2559818410551742 1106 10-11 0.33905452672380676 1106 12-13 0.3074948502772088 1106 14-15 0.26029627843597325 1106 16-17 0.27808673796780425 1106 18-19 0.3991460658106831 1106 20-21 0.4224678338804182 1106 22-23 0.5329389649442149 1106 24-25 0.36276805161461567 1106 26-27 0.5531653243181509 1106 28-29 0.43962606177739616 1106 30-31 0.3974524714316843 1106 32-33 0.7481351687316717 1106 34-35 0.7351868362589258 1106 36-37 0.4822540390073229 1106 38-39 0.19856760463532908 1106 40-41 0.8707409578330285 1106 42-43 0.5665390899417204 1106 44-45 0.5405209246385638 1106 46-47 0.6583209578047118 1106 48-49 0.666262748097779 1106 50-51 0.9930594235904238 1106 52-53 1.0248113055594033 1106 54-55 1.0196475929929107 1106 56-57 1.0153504717074924 1106 58-59 0.8794859678146096 1106 60-61 1.2333181889383624 1106 62-63 1.4016095350443152 1106 64-65 0.9963008957583952 1106 66-67 1.066825388102032 1106 68-69 1.4280283568510654 1106 70-71 1.3088433578105025 1106 72-73 1.4695340185250245 1106 74-75 1.4613021123312144 1106 76-77 1.3766156625038768 1106 78-79 1.4271599357800433 1106 80-81 1.262776329114331 1106 82-83 1.179275450224889 1106 84-85 1.057208598320365 1106 86-87 1.0505591990669636 1106 88-89 0.9608924564424264 1106 90-91 0.8963519570481182 1106 92-93 0.770051233410431 1106 94-95 0.7957977656216038 1106 96-97 1.239672969600317 1106 98-99 1.244090629324603 1106 100-101 1.4385719385051488 1107 1 0.16060631254051216 1107 2 0.138158471541594 1107 3 0.26162184202160077 1107 4 0.010524355159162724 1107 5 0.0259011145896082 1107 6 0.08983160885322405 1107 7 -0.09291374145725229 1107 8 0.3326865057890558 1107 9 0.30778257836493594 1107 10-11 0.08275667248427254 1107 12-13 -0.02342017004606589 1107 14-15 0.06375123637150182 1107 16-17 -0.24020421580855356 1107 18-19 0.016843863334081277 1107 20-21 0.44557965919472053 1107 22-23 0.3254051715800017 1107 24-25 0.4782917306012848 1107 26-27 0.4790798720069702 1107 28-29 0.39714803927999753 1107 30-31 0.28198787175307416 1107 32-33 0.5869786321970238 1107 34-35 0.19752968529947168 1107 36-37 0.45493576695891846 1107 38-39 0.31316964641621325 1107 40-41 0.5741628313560412 1107 42-43 -0.3446174465929204 1107 44-45 -0.0985753982653037 1107 46-47 0.3321677297113226 1107 48-49 0.8489950479370876 1107 50-51 0.7499008074641296 1107 52-53 0.5284387750744841 1107 54-55 0.7952880126923176 1107 56-57 0.8071195425081328 1107 58-59 0.6936933598176367 1107 60-61 0.4512263087984607 1107 62-63 0.8412777456502383 1107 64-65 0.4764975116956336 1107 66-67 0.5292745798970913 1107 68-69 0.6524115688748893 1107 70-71 0.7035049515339153 1107 72-73 0.7001914530651447 1107 74-75 0.49311041179241144 1107 76-77 0.5102648732043207 1107 78-79 0.45238680032300493 1107 80-81 -0.05990303566495214 1107 82-83 0.2174879466801336 1107 84-85 0.17074721255611536 1107 86-87 0.17864313894786576 1107 88-89 0.592946525825166 1107 90-91 0.7434783395039268 1107 92-93 0.32823253599101676 1107 94-95 0.40551985655647727 1107 96-97 0.6493525234333859 1107 98-99 0.21863926341100282 1107 100-101 -0.2846769508038598 1108 1 0.05320114534099574 1108 2 0.14857063779061974 1108 3 0.1716494925875729 1108 4 -0.15982905363717492 1108 5 0.15946630943969353 1108 6 0.12232102387093846 1108 7 0.11368783116868997 1108 8 0.19741475257036 1108 9 0.19947012696944455 1108 10-11 0.09849373288347607 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-0.5558506630118742 2316 96-97 -0.6681957726059018 2316 98-99 -0.23677694994428933 2316 100-101 -0.5132046934602172 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3750.0 3 712.0 4 551.0 5 490.0 6 502.0 7 575.0 8 699.0 9 827.0 10 1086.0 11 1490.0 12 2236.0 13 3186.0 14 2660.0 15 2357.0 16 2735.0 17 3483.0 18 3175.0 19 2091.0 20 1886.0 21 1844.0 22 1900.0 23 2090.0 24 2280.0 25 2640.0 26 2993.0 27 3540.0 28 4159.0 29 4887.0 30 6107.0 31 7614.0 32 9359.0 33 12236.0 34 17465.0 35 28061.0 36 41515.0 37 66554.0 38 70502.0 39 15606.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.095350153406216 17.075452027047152 11.473295403771113 22.355902415775518 2 15.733572338879693 20.197358234376303 41.61167951023714 22.457389916506866 3 19.505469964327617 28.339298582037554 28.174633896508393 23.980597557126437 4 11.707344669777193 15.835390309335445 37.2399500966208 35.21731492426656 5 12.262538559569323 37.63026881528329 34.305510891961745 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25.865657464946416 31.50787719261679 21.54920602781657 28-29 21.115935296359826 26.212299885648942 31.323405040975793 21.34835977701544 30-31 21.320230783547366 25.810980830076307 31.460562069607295 21.40822631676903 32-33 21.113597551079962 25.918382235800863 31.440512295966755 21.527507917152416 34-35 21.612326871666735 26.271324320733903 30.983609249034632 21.13273955856473 36-37 21.737211952961168 26.11995440906793 30.589750416570688 21.553083221400215 38-39 21.455237274750523 26.488650776557304 30.51621915906861 21.539892789623565 40-41 21.859245363181312 26.165001630628286 30.711013772077063 21.264739234113343 42-43 21.68485105950141 26.27737007906995 30.26751550435139 21.770263357077255 44-45 21.56930801200439 26.659355915873505 30.225665821890964 21.54567025023114 46-47 21.874495041683375 26.819346112643856 29.66312173460289 21.643037111069884 48-49 21.76967952276288 26.837705563128132 29.490968015117897 21.901646898991093 50-51 21.8448991807265 27.208409711570837 29.387602409187835 21.559088698514834 52-53 22.206162676218604 27.06741717613332 29.139248233699313 21.58717191394876 54-55 21.726979909548835 27.368134441008518 29.137662003479814 21.767223645962837 56-57 21.827086914309394 27.624416011058205 28.80772757804097 21.740769496591433 58-59 21.843949987882922 27.24981226008934 29.053760931548982 21.85247682047876 60-61 21.883587309517864 27.404297076593753 29.17916353154296 21.532952082345428 62-63 21.789933326651536 27.249010072058677 29.51518587608062 21.44587072520917 64-65 21.75837130879964 27.30268965042623 29.141819291636622 21.79711974913751 66-67 21.65447137446826 27.654046583423575 28.94472331743857 21.74675872466959 68-69 21.96033841757617 27.405540054308393 29.060973511568584 21.573148016546853 70-71 21.8297787045375 27.26388818251715 28.980221923717835 21.926111189227516 72-73 21.77370012281833 27.875123229718145 28.6506289764491 21.700547671014426 74-75 21.705591047143137 27.94745582668801 28.59423390535466 21.752719220814196 76-77 21.81643289690993 27.70216496325905 28.591367672754252 21.89003446707676 78-79 21.90409655568587 27.84428181622346 28.558605998228774 21.693015629861893 80-81 21.68919646704007 27.890277179376703 28.28312748336445 22.137398870218775 82-83 22.010139860662935 28.09959144350147 28.321447645377145 21.568821050458446 84-85 21.90020270624051 28.1788602074934 28.189481715298708 21.73145537096738 86-87 21.78644413659389 28.025055577663615 28.229563427354083 21.958936858388412 88-89 21.879661785367475 28.09153520774823 28.06654766411355 21.962255342770746 90-91 21.96790448268689 28.119420586567056 28.201881791638044 21.710793139108013 92-93 21.886891837495117 28.122460767691766 28.344678666932033 21.645968727881087 94-95 21.732560992394276 28.106767358222978 28.262053365726974 21.898618283655775 96-97 22.11639847625771 27.910447984421577 28.03293963509752 21.940213904223196 98-99 21.764800890117186 28.005640998031932 27.907985332208725 22.32157277964216 100-101 21.86154405287332 28.07975268352435 27.943351724529887 22.115351539072446 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 270.0 1 209.0 2 1060.5 3 1519.0 4 692.5 5 387.0 6 572.5 7 882.0 8 1168.0 9 1171.0 10 942.0 11 749.5 12 689.0 13 727.5 14 767.5 15 768.0 16 809.0 17 828.5 18 829.0 19 885.5 20 920.0 21 1021.0 22 1158.0 23 1309.0 24 1508.0 25 1774.5 26 2221.0 27 2718.5 28 3143.5 29 3619.0 30 4178.0 31 4883.0 32 5839.5 33 6490.5 34 7000.5 35 7595.0 36 8354.0 37 9052.5 38 9142.0 39 9133.5 40 9251.5 41 9478.5 42 9573.0 43 9649.0 44 10076.0 45 10704.5 46 11083.0 47 11173.5 48 11284.5 49 11240.5 50 10979.0 51 10732.0 52 10554.5 53 10528.0 54 10449.0 55 10053.5 56 9344.5 57 8527.5 58 8006.0 59 7242.0 60 6105.5 61 5175.5 62 4320.0 63 3276.0 64 2351.5 65 1774.0 66 1308.0 67 1029.0 68 861.0 69 655.5 70 490.0 71 348.5 72 307.0 73 293.0 74 228.0 75 178.5 76 119.0 77 65.5 78 52.0 79 39.5 80 25.5 81 13.5 82 8.5 83 11.0 84 7.0 85 2.5 86 2.5 87 3.5 88 3.0 89 2.5 90 3.5 91 2.5 92 2.0 93 3.0 94 3.5 95 2.5 96 1.0 97 2.0 98 2.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046149494588599585 2 0.0086344215681896 3 0.009229898917719918 4 0.0047638187962425384 5 0.006252512170068331 6 0.005061557471007697 7 0.0038706027719470617 8 0.01071859229154571 9 0.015482411087788247 10-11 0.0026796480728864275 12-13 0.009676506929867655 14-15 0.009229898917719918 16-17 0.019055275184970154 18-19 0.011314069641076029 20-21 0.0038706027719470617 22-23 0.010420853616780553 24-25 0.022330400607386898 26-27 0.006550250844833489 28-29 0.016971104461614043 30-31 0.01563128042517083 32-33 0.012951632352284399 34-35 0.2648385512036086 36-37 0.4728090155270719 38-39 0.4671519807065339 40-41 0.48769594926532983 42-43 0.47757283432331443 44-45 0.4924597680615724 46-47 0.4999032349307013 48-49 0.5036249683652658 50-51 0.5143435606568115 52-53 0.5037738377026484 54-55 0.4917154213746595 56-57 0.4863561252288866 58-59 0.4841230851681479 60-61 0.4806990904083486 62-63 0.483378738481235 64-65 0.49365072276063304 66-67 0.4713203221532461 68-69 0.4949905467970762 70-71 0.47816831167284474 72-73 0.48576064787935624 74-75 0.49722358685781487 76-77 0.48620725589150404 78-79 0.48620725589150404 80-81 0.48858916528962526 82-83 0.4881425572774776 84-85 0.4875470799279472 86-87 0.4906733360129814 88-89 0.5057091390886219 90-91 0.526550846322183 92-93 0.5161299927054025 94-95 0.48918464263915556 96-97 0.4634302472719694 98-99 0.45464695636639724 100-101 0.4634302472719694 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 335865.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.29948470233343 #Duplication Level Percentage of deduplicated Percentage of total 1 87.0333807259978 62.92468578391638 2 8.167591461515416 11.810253078534858 3 2.0077993651688617 4.354885784621426 4 0.8646994159172959 2.5006928877291674 5 0.502162175117058 1.8153033248983108 6 0.3149520071427953 1.3662520693433842 7 0.21723531096439208 1.0994200729313697 8 0.14464137728071255 0.8365997635225051 9 0.1290524374081102 0.8397382251767855 >10 0.5764113910016491 7.129944955534154 >50 0.029287035357950193 1.4863815243858052 >100 0.010724829849321209 1.491451445308563 >500 8.249869114862467E-4 0.5833391375771945 >1k 0.0012374803672293702 1.761051946520109 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2428 0.7229095023298051 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2028 0.6038140324237417 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1449 0.4314233397347148 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 984 0.2929748559689161 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 972 0.28940199187173415 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 378 0.11254521906122997 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 348 0.10361305881827519 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 340 0.10123114942015395 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 2.977386747651586E-4 0.0 0.0 0.0 0.0 7 2.977386747651586E-4 0.0 0.0 0.0 0.0 8 2.977386747651586E-4 0.0 0.0 0.0 0.0 9 2.977386747651586E-4 0.0 0.0 0.0 0.0 10-11 2.977386747651586E-4 0.0 0.0 0.0 0.0 12-13 2.977386747651586E-4 0.0 0.0 0.0 5.954773495303172E-4 14-15 2.977386747651586E-4 0.0 0.0 0.0 5.954773495303172E-4 16-17 2.977386747651586E-4 0.0 0.0 0.0 5.954773495303172E-4 18-19 2.977386747651586E-4 0.0 0.0 0.0 5.954773495303172E-4 20-21 2.977386747651586E-4 0.0 0.0 0.0 5.954773495303172E-4 22-23 2.977386747651586E-4 0.0 0.0 2.977386747651586E-4 8.932160242954758E-4 24-25 5.954773495303172E-4 0.0 0.0 5.954773495303172E-4 8.932160242954758E-4 26-27 5.954773495303172E-4 0.0 0.0 7.443466869128965E-4 8.932160242954758E-4 28-29 5.954773495303172E-4 0.0 0.0 0.0016375627112083725 8.932160242954758E-4 30-31 5.954773495303172E-4 0.0 0.0 0.003721733434564483 8.932160242954758E-4 32-33 5.954773495303172E-4 0.0 0.0 0.005359296145772855 8.932160242954758E-4 34-35 5.954773495303172E-4 0.0 0.0 0.008038944218659283 8.932160242954758E-4 36-37 5.954773495303172E-4 0.0 0.0 0.013844848376579875 8.932160242954758E-4 38-39 5.954773495303172E-4 0.0 0.0 0.025456656692421063 8.932160242954758E-4 40-41 5.954773495303172E-4 0.0 0.0 0.03617524898396677 8.932160242954758E-4 42-43 7.443466869128965E-4 0.0 0.0 0.04510740922692153 8.932160242954758E-4 44-45 8.932160242954758E-4 0.0 0.0 0.056868086880145295 8.932160242954758E-4 46-47 8.932160242954758E-4 0.0 0.0 0.07473240736605481 0.001042085361678055 48-49 0.0011909546990606344 0.0 0.0 0.09095916514075596 0.0011909546990606344 50-51 0.0011909546990606344 0.0 0.0 0.11224748038646479 0.0011909546990606344 52-53 0.0011909546990606344 0.0 0.0 0.13070727822190464 0.0011909546990606344 54-55 0.0011909546990606344 0.0 0.0 0.15705715093862116 0.0011909546990606344 56-57 0.0011909546990606344 0.0 0.0 0.18712875708990218 0.0011909546990606344 58-59 0.0011909546990606344 0.0 0.0 0.21988001131406965 0.0011909546990606344 60-61 0.0011909546990606344 0.0 0.0 0.25947925505783576 0.0011909546990606344 62-63 0.0011909546990606344 0.0 0.0 0.30101380018757534 0.0011909546990606344 64-65 0.0011909546990606344 0.0 0.0 0.35073615887335685 0.0011909546990606344 66-67 0.0011909546990606344 0.0 0.0 0.4015006029208164 0.0011909546990606344 68-69 0.0011909546990606344 0.0 0.0 0.46164381522337844 0.0011909546990606344 70-71 0.0011909546990606344 0.0 0.0 0.520893811501645 0.0011909546990606344 72-73 0.0011909546990606344 0.0 0.0 0.5835678025397109 0.0013398240364432138 74-75 0.0011909546990606344 0.0 0.0 0.64758161761422 0.0014886933738257932 76-77 0.0011909546990606344 0.0 0.0 0.7176990755214148 0.0014886933738257932 78-79 0.0011909546990606344 0.0 0.0 0.804341029878076 0.0014886933738257932 80-81 0.0011909546990606344 0.0 0.0 0.9049767019486996 0.0014886933738257932 82-83 0.0014886933738257932 0.0 0.0 1.0063567207062363 0.0014886933738257932 84-85 0.0014886933738257932 0.0 0.0 1.1116073422357196 0.0014886933738257932 86-87 0.0014886933738257932 0.0 0.0 1.235020022925878 0.0014886933738257932 88-89 0.0014886933738257932 0.0 0.0 1.3734685066916767 0.0014886933738257932 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAACGC 35 3.4759323E-6 67.64445 3 TAACGCA 45 2.0881089E-7 63.13481 4 TACCTGG 460 0.0 54.548588 2 GTACCTG 505 0.0 50.640415 1 GTATCAA 1500 0.0 43.56951 1 TATAACG 55 5.0528775E-5 43.04005 2 GTACACG 90 1.2241799E-8 42.096146 1 GGTATCA 770 0.0 41.207752 1 GTACATG 1885 0.0 33.91699 1 ATCAACG 1925 0.0 33.699234 3 TACATGG 1905 0.0 33.30238 2 TATCAAC 1955 0.0 33.177162 2 ACATGGG 1885 0.0 32.404736 3 CAACGCA 2020 0.0 31.879957 5 TCAACGC 2025 0.0 31.80124 4 ACCTGGG 760 0.0 31.775087 3 ACACGGG 105 1.9434083E-6 31.567408 3 AACGCAG 2075 0.0 31.491343 6 GTGTATC 125 2.2032145E-7 30.309227 1 CATGGGT 300 0.0 28.410666 4 >>END_MODULE