FastQCFastQC Report
Thu 26 May 2016
SRR936372_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936372_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335865
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22870.6809283491879178No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19020.5662989594033316No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13860.4126658032245098No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9300.2768969675315975No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8650.25754395367186217No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3440.10242210411921457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG5000.056.0101972
GTACACG701.2641976E-954.4092331
GTACCTG5850.048.0149881
ACACGGG804.2527972E-947.445063
GGTATCA9650.042.9212761
GTATCAA16800.039.106641
TAACGCA500.001623390237.956054
GTACATG21600.033.9428021
TACATGG21850.033.4544832
TATCAAC20350.031.9551772
ATCAACG20200.031.9432133
TCAACGC20200.031.9432134
TACACGG1201.5098158E-731.6441762
CAACGCA20450.031.552715
CATGGGA11000.031.4862694
ACATGGG22000.031.4862693
AACGCAG21150.030.9570646
ACCTGGG8850.028.4134313
ATGGGAT4700.028.2651445
ACGCAGA24100.026.9708467
Produced by FastQC (version 0.11.5)