##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936372_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 335865 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.471540648772574 34.0 31.0 34.0 31.0 34.0 2 32.568942878835244 34.0 31.0 34.0 31.0 34.0 3 32.659351227427685 34.0 31.0 34.0 31.0 34.0 4 36.1525821386569 37.0 35.0 37.0 35.0 37.0 5 36.12978726571688 37.0 35.0 37.0 35.0 37.0 6 36.18539294061602 37.0 37.0 37.0 35.0 37.0 7 36.14743721435696 37.0 36.0 37.0 35.0 37.0 8 36.135206109597604 37.0 36.0 37.0 35.0 37.0 9 37.90590266922722 39.0 38.0 39.0 35.0 39.0 10-11 37.83225403063731 39.0 38.0 39.0 35.0 39.0 12-13 37.77173715629792 39.0 38.0 39.0 35.0 39.0 14-15 39.08791776457803 40.5 38.5 41.0 36.0 41.0 16-17 39.020453158262995 40.0 38.0 41.0 35.5 41.0 18-19 39.07472794128593 40.0 38.5 41.0 36.0 41.0 20-21 39.00303097970911 40.0 38.5 41.0 35.0 41.0 22-23 38.82478972206095 40.0 38.0 41.0 35.0 41.0 24-25 38.94084676879103 40.0 38.0 41.0 35.0 41.0 26-27 38.82673395560717 40.0 38.0 41.0 35.0 41.0 28-29 38.70117606776532 40.0 38.0 41.0 35.0 41.0 30-31 38.50210203504385 40.0 38.0 41.0 34.5 41.0 32-33 38.214486475220696 40.0 38.0 41.0 34.0 41.0 34-35 37.94449257886353 40.0 37.5 41.0 33.0 41.0 36-37 37.728806216783525 40.0 37.0 41.0 33.0 41.0 38-39 37.5559093683474 40.0 37.0 41.0 33.0 41.0 40-41 37.390016822235125 40.0 37.0 41.0 33.0 41.0 42-43 37.35563544876662 40.0 37.0 41.0 33.0 41.0 44-45 37.163720542479865 40.0 37.0 41.0 32.0 41.0 46-47 36.87780060440951 40.0 36.0 41.0 31.0 41.0 48-49 36.58512944188885 40.0 36.0 41.0 31.0 41.0 50-51 36.355355574412336 40.0 35.5 41.0 30.0 41.0 52-53 36.12981108481087 40.0 35.0 41.0 30.0 41.0 54-55 35.93302666249832 39.0 35.0 41.0 29.0 41.0 56-57 35.69217245024042 39.0 35.0 41.0 28.5 41.0 58-59 35.43509892367469 39.0 35.0 41.0 28.0 41.0 60-61 35.16729787265717 38.0 35.0 40.0 28.0 41.0 62-63 34.87102556086523 38.0 35.0 40.0 27.0 41.0 64-65 34.52102481651854 37.0 34.0 40.0 26.0 41.0 66-67 34.188030905274445 37.0 34.0 40.0 26.0 41.0 68-69 33.840556473583135 36.0 34.0 39.0 26.0 41.0 70-71 33.47212421657511 36.0 34.0 39.0 25.5 40.5 72-73 33.06822383993568 35.0 33.0 38.5 24.5 40.0 74-75 32.65785211320025 35.0 33.0 37.0 24.0 39.5 76-77 31.946637786015216 35.0 32.0 37.0 22.5 39.0 78-79 31.997515370759082 35.0 33.0 36.0 22.5 39.0 80-81 31.828548672829857 35.0 33.0 36.0 22.0 38.5 82-83 31.53995504146011 35.0 33.0 36.0 21.5 37.0 84-85 31.234097033034104 35.0 32.0 35.0 20.0 37.0 86-87 30.833020707724828 35.0 32.0 35.0 18.5 36.5 88-89 30.33541303797657 35.0 31.0 35.0 9.5 36.0 90-91 30.095496702544178 34.0 31.0 35.0 7.5 36.0 92-93 29.79924820984622 34.0 31.0 35.0 2.0 35.5 94-95 29.634967918657793 34.0 31.0 35.0 2.0 35.0 96-97 29.36528962529588 34.0 31.0 35.0 2.0 35.0 98-99 28.922025218465755 34.0 30.5 35.0 2.0 35.0 100-101 25.76198621469936 31.0 24.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09217999161046464 1101 2 -0.024798435737466207 1101 3 0.2162359751020304 1101 4 0.10893647353697844 1101 5 0.1901781570527632 1101 6 0.17311330812587045 1101 7 0.057086142754968705 1101 8 0.024366905785456083 1101 9 0.4246759379463043 1101 10-11 0.1395860768987589 1101 12-13 0.17435405089702272 1101 14-15 0.08789369432108884 1101 16-17 0.2526411304219991 1101 18-19 0.47181185665389336 1101 20-21 0.46815465610603724 1101 22-23 0.17375181949328322 1101 24-25 0.04269955840771189 1101 26-27 -0.047687335724695856 1101 28-29 0.01940972161256127 1101 30-31 -0.08838438418097638 1101 32-33 0.1431768132708342 1101 34-35 -0.09074937381935655 1101 36-37 -0.2137117137133302 1101 38-39 0.013242534275157425 1101 40-41 -0.11959347329992909 1101 42-43 0.07029448206252198 1101 44-45 -0.4486572730684628 1101 46-47 -0.322794665777252 1101 48-49 -0.7410380886726813 1101 50-51 -0.3192794742136371 1101 52-53 -0.20317915865765457 1101 54-55 -0.27651762952843484 1101 56-57 -0.5879444865716437 1101 58-59 -0.8235152143579043 1101 60-61 -0.776711815498544 1101 62-63 -0.4066206851821761 1101 64-65 -0.48052646111209896 1101 66-67 -0.5590765127241966 1101 68-69 -0.922034322597284 1101 70-71 -0.9913433129595219 1101 72-73 -0.913816558211856 1101 74-75 -0.5471039269535396 1101 76-77 -0.6055827125552469 1101 78-79 -0.5958482061596833 1101 80-81 -0.7084905658640928 1101 82-83 -0.5791808160528227 1101 84-85 -0.24037059888543766 1101 86-87 -0.4759357687912811 1101 88-89 -0.4500225456485296 1101 90-91 -0.6002285056919199 1101 92-93 -0.6396849519271193 1101 94-95 -0.07499722166369338 1101 96-97 -0.3739369780106898 1101 98-99 -0.5067213963413266 1101 100-101 -0.16156148032594686 1102 1 0.08526589403489027 1102 2 0.3224030009581327 1102 3 0.24034552314154212 1102 4 0.23249229514727432 1102 5 0.2515220660620443 1102 6 0.2916556607149161 1102 7 0.20430254502974066 1102 8 0.1951989919871906 1102 9 0.32434669520461057 1102 10-11 0.3029802702540394 1102 12-13 0.4013893696638604 1102 14-15 0.46422563086703406 1102 16-17 0.5525812681053353 1102 18-19 0.45134642714177176 1102 20-21 0.5831576492218744 1102 22-23 0.5940750760033069 1102 24-25 0.3748531052499757 1102 26-27 0.6732495095312245 1102 28-29 0.5719239389127679 1102 30-31 0.9252791895993298 1102 32-33 1.0595491568805357 1102 34-35 1.0789977078927038 1102 36-37 1.1098964874240593 1102 38-39 1.0230075746822251 1102 40-41 0.7801670774333829 1102 42-43 0.7912238445288438 1102 44-45 0.9803983988860381 1102 46-47 1.1505217065663516 1102 48-49 1.1830041142605765 1102 50-51 0.9367067574535213 1102 52-53 1.1522084498427958 1102 54-55 1.0550447769366968 1102 56-57 1.0978007992709138 1102 58-59 0.9677748185663617 1102 60-61 0.9768425095538333 1102 62-63 1.2825741666585913 1102 64-65 1.1849180165891866 1102 66-67 1.174408970664011 1102 68-69 1.489414345466166 1102 70-71 1.4422618950620247 1102 72-73 1.1868419272715371 1102 74-75 1.2097054115678603 1102 76-77 1.1734879249784314 1102 78-79 1.3556483517571039 1102 80-81 1.3787063811577553 1102 82-83 1.4311678819417857 1102 84-85 1.2638496944399158 1102 86-87 1.0336122707178532 1102 88-89 1.0783521924538348 1102 90-91 1.261504508375726 1102 92-93 1.2836688343644163 1102 94-95 1.3766714404135278 1102 96-97 1.0439917858324712 1102 98-99 0.97133906459851 1102 100-101 1.5370841347593611 1103 1 0.2072439160129136 1103 2 0.3636117921669282 1103 3 0.17715870995473182 1103 4 0.2135362511912291 1103 5 0.1828407473807232 1103 6 -0.00916851510925909 1103 7 0.24798386371105607 1103 8 0.2762429480311468 1103 9 0.241379662237577 1103 10-11 0.24982092959470492 1103 12-13 0.2965816773561656 1103 14-15 0.41820914735055226 1103 16-17 0.18197687250093253 1103 18-19 0.12373653703188126 1103 20-21 0.338652154716371 1103 22-23 0.6178388122670384 1103 24-25 0.4799355228323918 1103 26-27 0.4769583007400158 1103 28-29 0.5855228400116701 1103 30-31 0.4041253434454859 1103 32-33 0.28592278325415776 1103 34-35 0.37899770789270093 1103 36-37 0.45138000390757327 1103 38-39 0.18097460764925444 1103 40-41 0.4406066378729392 1103 42-43 0.25619637200136935 1103 44-45 0.373667629655273 1103 46-47 0.3295052230498712 1103 48-49 0.3194052131616658 1103 50-51 0.10964631789308044 1103 52-53 0.07446119709553756 1103 54-55 0.11781950221142523 1103 56-57 0.2623612388313603 1103 58-59 0.4930495438410887 1103 60-61 0.2747820699933925 1103 62-63 0.4063379029223242 1103 64-65 0.2062092253803911 1103 66-67 0.18168919044423149 1103 68-69 0.41839786194968553 1103 70-71 -0.002106236806106665 1103 72-73 -0.0632954353658235 1103 74-75 0.1936339829964311 1103 76-77 0.26030111179161963 1103 78-79 0.265401099009857 1103 80-81 0.2366734141247946 1103 82-83 0.5373492006231082 1103 84-85 0.2075310131212298 1103 86-87 0.3592990839046628 1103 88-89 0.34662142322306266 1103 90-91 0.23210890398011585 1103 92-93 0.4688336695292499 1103 94-95 0.582715396457484 1103 96-97 0.5257225550632398 1103 98-99 0.2448280755875203 1103 100-101 0.2401061127813371 1104 1 -0.06801740802890066 1104 2 -0.08847880016756449 1104 3 -0.08451376391286658 1104 4 0.06449443934453569 1104 5 0.021168273513126223 1104 6 -0.13625910744375602 1104 7 0.07019640726506537 1104 8 -0.06914032723017272 1104 9 -0.10443915309343765 1104 10-11 0.001345319838605974 1104 12-13 -0.1347336536542869 1104 14-15 0.07208545397076449 1104 16-17 0.25558314427794215 1104 18-19 0.15923305271132193 1104 20-21 0.1615615623818769 1104 22-23 0.282952052685161 1104 24-25 -0.09981186392719366 1104 26-27 -0.07926992225650054 1104 28-29 0.14323189924512292 1104 30-31 0.03365496017021741 1104 32-33 -0.29386815751239226 1104 34-35 -0.4693472398424845 1104 36-37 -0.7855537940018351 1104 38-39 -0.598642117089419 1104 40-41 -0.6374421426148658 1104 42-43 -0.7087426523888709 1104 44-45 -0.49205536686041285 1104 46-47 -0.7195801428037925 1104 48-49 -1.0528508843993052 1104 50-51 -1.0246306855912408 1104 52-53 -0.6752774788626468 1104 54-55 -0.9574853758373578 1104 56-57 -0.8669070538515697 1104 58-59 -0.8222814666118765 1104 60-61 -1.4222127035257657 1104 62-63 -0.8337753374957941 1104 64-65 -1.1663953042363318 1104 66-67 -1.1633892067683078 1104 68-69 -1.1859139847402105 1104 70-71 -0.9029337629385097 1104 72-73 -0.9235306270034513 1104 74-75 -0.9610262957492139 1104 76-77 -1.033862651274589 1104 78-79 -0.9760187616173255 1104 80-81 -1.0056610806487285 1104 82-83 -0.8425724021643468 1104 84-85 -1.2108487778195354 1104 86-87 -1.1716678150500428 1104 88-89 -0.6424465210278072 1104 90-91 -0.35630573016622336 1104 92-93 -0.5762011736763277 1104 94-95 -0.6347933143439093 1104 96-97 -0.9362547968531381 1104 98-99 -1.139274711868925 1104 100-101 -0.8236569534207554 1105 1 0.05148662982084318 1105 2 0.09497825179425945 1105 3 0.07830538936227782 1105 4 0.0913551714014531 1105 5 0.08413076171421352 1105 6 0.12459880452762206 1105 7 0.033232310916027075 1105 8 -0.07654013844759078 1105 9 -0.004081398440874295 1105 10-11 -0.050196987271036164 1105 12-13 -0.008226014951524974 1105 14-15 0.02384101548242512 1105 16-17 0.15993912763710938 1105 18-19 -0.07164688389502061 1105 20-21 0.1340773816633387 1105 22-23 0.1335399589464572 1105 24-25 0.22844842297572399 1105 26-27 0.305858205183398 1105 28-29 -0.02523325172268187 1105 30-31 0.15153337020134217 1105 32-33 0.14182340437217533 1105 34-35 0.12122178816026263 1105 36-37 0.3529566880929522 1105 38-39 0.32944570177252075 1105 40-41 0.3223075456608129 1105 42-43 0.4321101321542642 1105 44-45 0.32145191059171907 1105 46-47 0.08981936542922853 1105 48-49 0.10181682329066888 1105 50-51 0.03787732601539773 1105 52-53 0.14000109198325816 1105 54-55 0.23481066322431587 1105 56-57 0.21343625077593487 1105 58-59 0.08550057107472497 1105 60-61 0.2297689309585209 1105 62-63 0.321370153280661 1105 64-65 0.2994665115724615 1105 66-67 0.33519492384126437 1105 68-69 0.33774210466349786 1105 70-71 0.07160972114898101 1105 72-73 0.10924995402739057 1105 74-75 0.27826728447755755 1105 76-77 0.17139762397508207 1105 78-79 0.06283898720861458 1105 80-81 0.3129037055724737 1105 82-83 0.35449564113911336 1105 84-85 0.35950186835295384 1105 86-87 0.3175587590121651 1105 88-89 0.23152944997891467 1105 90-91 0.5426081873054862 1105 92-93 0.9924480985784605 1105 94-95 0.2981597347279106 1105 96-97 0.3426539931903285 1105 98-99 0.48204140573563237 1105 100-101 0.8353282819165493 1106 1 0.11711927555270307 1106 2 0.07382272217651575 1106 3 0.02049523919730234 1106 4 -0.04195272292190566 1106 5 -0.04592243574487753 1106 6 0.045217870317394215 1106 7 0.1782571139507496 1106 8 0.047935181971702434 1106 9 0.09972098534399976 1106 10-11 0.2461656084058248 1106 12-13 -0.012227047476045527 1106 14-15 0.3750284186832502 1106 16-17 0.1909653672276832 1106 18-19 0.10977440855633347 1106 20-21 0.3344095852053499 1106 22-23 0.05982107497078459 1106 24-25 0.2560740654977778 1106 26-27 0.07500480121940001 1106 28-29 -0.08483909670933087 1106 30-31 0.06167567901595561 1106 32-33 0.23213563080928878 1106 34-35 0.36842723809404987 1106 36-37 0.09184021483758187 1106 38-39 0.21692858655625713 1106 40-41 0.10685783633890367 1106 42-43 0.10117479961402864 1106 44-45 0.12878507931969807 1106 46-47 0.1890090581409538 1106 48-49 0.32919668008401004 1106 50-51 0.33151832172817564 1106 52-53 0.2872368442671629 1106 54-55 0.3213070381654006 1106 56-57 0.3511435974123742 1106 58-59 0.26558070395613953 1106 60-61 0.5000097785264686 1106 62-63 0.5271792260287427 1106 64-65 0.47880030879362323 1106 66-67 0.7484796985938047 1106 68-69 0.8604520326112421 1106 70-71 0.6459402636732818 1106 72-73 0.6975027429659093 1106 74-75 1.0670879619034324 1106 76-77 0.969241667879821 1106 78-79 0.8067841287318132 1106 80-81 0.7165871245754118 1106 82-83 0.6314647327419927 1106 84-85 0.9055894982602517 1106 86-87 0.8470867157359194 1106 88-89 0.7272422285921891 1106 90-91 0.8355365166359192 1106 92-93 0.8345335736519708 1106 94-95 0.9611190397940135 1106 96-97 1.0081889980162622 1106 98-99 0.9184738090486881 1106 100-101 1.0577475317650382 1107 1 -0.08450658700519398 1107 2 0.00848100745867697 1107 3 0.006434363878270233 1107 4 -0.2779627427725515 1107 5 -0.14241076167360234 1107 6 -0.001572941668619876 1107 7 0.17499593614566322 1107 8 -0.025819426214326313 1107 9 -0.1343748649254053 1107 10-11 0.01755231792468237 1107 12-13 0.08549012806039258 1107 14-15 -0.1634558425890873 1107 16-17 -0.0805985802153586 1107 18-19 -0.08589625974880022 1107 20-21 0.09881312051113866 1107 22-23 0.0031758343998404825 1107 24-25 -0.06209164014547497 1107 26-27 -0.001940089602037176 1107 28-29 0.0401254557058337 1107 30-31 -0.3948183185263474 1107 32-33 -0.0705158485365871 1107 34-35 0.06667376423073534 1107 36-37 -0.11778498603206344 1107 38-39 -0.42566523138494716 1107 40-41 0.09674345879849255 1107 42-43 -0.10585091170084127 1107 44-45 -0.0560858914714899 1107 46-47 -0.3560330063263848 1107 48-49 -0.3017466983071486 1107 50-51 -0.3104492555475602 1107 52-53 0.011986348000966984 1107 54-55 0.03635571951524952 1107 56-57 -0.10328262434771318 1107 58-59 -0.36006916843255254 1107 60-61 -0.10658110908105556 1107 62-63 0.04484393913963203 1107 64-65 -0.05749801808037347 1107 66-67 -0.36592147353967164 1107 68-69 -0.4439009308068549 1107 70-71 -0.45037082433125875 1107 72-73 -0.5703628397923879 1107 74-75 -0.4769143067419961 1107 76-77 -0.6072542202003355 1107 78-79 -0.49372867966217626 1107 80-81 -0.34743121364181206 1107 82-83 -0.19637816356200588 1107 84-85 0.051444494006542385 1107 86-87 0.0428755426571783 1107 88-89 0.2397552260399678 1107 90-91 -0.3081325447120342 1107 92-93 -0.056417839525078506 1107 94-95 0.03734316305708063 1107 96-97 -0.41283378296492756 1107 98-99 -0.5202373167666039 1107 100-101 -0.5653264827921021 1108 1 -0.2647105099292446 1108 2 -0.17122606935804896 1108 3 -0.06236802098304395 1108 4 -0.17062238158703735 1108 5 -0.03731700897335344 1108 6 -0.10638586830820884 1108 7 -0.046122183615850076 1108 8 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70-71 0.17580533492071027 2316 72-73 -0.06586403065362845 2316 74-75 -0.26837392037368346 2316 76-77 0.05165959673047382 2316 78-79 -0.05974429317625152 2316 80-81 -0.14550672306578605 2316 82-83 -0.27310785276192107 2316 84-85 -0.039538775128097114 2316 86-87 -0.33041535092981533 2316 88-89 -0.35204767907338663 2316 90-91 0.243702998820563 2316 92-93 -0.1807562499458477 2316 94-95 0.08492983129195864 2316 96-97 0.11760668867493251 2316 98-99 -0.051850272160766764 2316 100-101 -0.2765058764820125 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 1.0 4 4.0 5 8.0 6 20.0 7 62.0 8 101.0 9 190.0 10 392.0 11 769.0 12 1302.0 13 2118.0 14 1668.0 15 1727.0 16 2139.0 17 2962.0 18 2711.0 19 1674.0 20 1348.0 21 1358.0 22 1436.0 23 1491.0 24 1712.0 25 2070.0 26 2607.0 27 3096.0 28 3800.0 29 4782.0 30 5960.0 31 7298.0 32 9170.0 33 11716.0 34 16644.0 35 26439.0 36 40554.0 37 73007.0 38 85098.0 39 18408.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.48272259123815 17.465183968952907 11.8346145234719 22.21747891633704 2 15.93319873020411 20.423046914550834 41.13018981423577 22.513564541009284 3 19.352418382385782 28.153871347118635 28.65526327542316 23.838446995072424 4 11.92890000446608 15.956708796689146 37.09287957959299 35.02151161925178 5 12.151310794515654 37.73688833311003 34.429011656469115 15.6827892159052 6 29.713724264213297 34.791955101007844 20.59041579205931 14.903904842719545 7 28.104148988432854 32.11975049499055 22.726095306149794 17.050005210426807 8 27.18800708618046 33.178509222455446 21.693537582064224 17.939946109299868 9 27.803730665594806 15.792952525568307 21.57354889613386 34.82976791270302 10-11 25.086418650350588 26.016703139654325 28.490316049603265 20.406562160391825 12-13 25.782382802614144 23.747190091256904 29.220222410790047 21.2502046953389 14-15 22.373870453902608 24.753993419975288 28.078841201077815 24.793294925044286 16-17 21.524167664100137 27.78745437114053 28.61041141897565 22.077966545783685 18-19 21.423041996040077 25.907730784690276 31.42929450821014 21.239932711059502 20-21 22.286484152859035 25.4358149851875 31.22355708394742 21.054143778006043 22-23 21.65289625295878 25.53213344647403 31.340866121804893 21.474104178762303 24-25 21.273412365808305 25.743813895046504 31.720973579254046 21.26180015989114 26-27 21.0017090657901 26.082309084845885 31.28245438521271 21.633527464151303 28-29 21.140928606732608 26.231472824824774 31.32707870588165 21.30051986256096 30-31 21.338025757717862 25.85388874249875 31.305854662873422 21.502230836909966 32-33 21.079358179225085 26.018960450429795 31.338039701420545 21.56364166892457 34-35 21.59892454229032 26.304498640790573 30.856584796505654 21.23999202041345 36-37 21.682967859110057 26.101558661962393 30.629866166465693 21.585607312461853 38-39 21.47350870141277 26.42103225998541 30.480550221070967 21.62490881753085 40-41 21.8579869500556 26.1554173049468 30.6040995821219 21.382496162875693 42-43 21.709309838647727 26.343237585938915 30.262260718358547 21.685191857054807 44-45 21.60924092016174 26.6465487979896 30.164744204326883 21.57946607752178 46-47 21.809329541563063 26.80746839777854 29.616306597382486 21.76689546327591 48-49 21.80323841429369 26.87273404057324 29.43785594639866 21.886171598734414 50-51 21.85453830830661 27.187865686440905 29.28454238761795 21.673053617634537 52-53 22.006127991043247 27.167426958396362 29.179420907824056 21.647024142736335 54-55 21.65203527017592 27.17435156141837 29.34040364492228 21.83320952348343 56-57 21.807010805322566 27.48032621414726 28.837169833230607 21.87549314729956 58-59 21.83325567235304 27.141098339642976 29.13269308926447 21.89295289873951 60-61 21.838233433225998 27.366570346914866 29.23073716617465 21.56445905368449 62-63 21.882452771202715 27.20765783871496 29.444419632888213 21.46546975719411 64-65 21.725259592839333 27.24626893445979 29.149279839219922 21.879191633480964 66-67 21.603769371622526 27.69222753189526 28.845220549923333 21.858782546558885 68-69 21.930239828502522 27.39478659580486 29.160823545174402 21.514150030518213 70-71 21.851934557039286 27.194110729013143 28.991410239233023 21.962544474714544 72-73 21.773312332707086 27.709810250012655 28.594992601149873 21.921884816130387 74-75 21.70425617435577 27.947836184181146 28.51264645021065 21.835261191252435 76-77 21.71555656321877 27.67273551724447 28.682851594080113 21.928856325456657 78-79 21.79511061106185 27.697695385555367 28.756491740494305 21.75070226288848 80-81 21.763383552670756 27.835556481867446 28.31194545346275 22.089114511999046 82-83 21.887663198011108 28.108019591204798 28.300656513777856 21.70366069700624 84-85 21.915134661162412 28.08985251361052 28.145530226000897 21.84948259922617 86-87 21.599856916094705 27.968432113393995 28.329868020001935 22.101842950509365 88-89 21.783687237154737 28.02804481301212 28.160693245260365 22.02757470457278 90-91 21.79768972561012 28.20260891378878 28.209776259362346 21.789925101238754 92-93 21.855409995147987 28.084350390027247 28.2926137423954 21.767625872429367 94-95 21.46373706862542 28.031513177385186 28.381361849225645 22.123387904763746 96-97 21.890282845696575 27.810554868253963 28.24228944379188 22.056872842257587 98-99 21.572267587705912 27.92163226416358 28.076462137396625 22.42963801073388 100-101 21.425913023801442 28.009718277149542 28.21039593642627 22.353972762622746 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 271.0 1 228.0 2 1016.5 3 1388.0 4 637.5 5 396.5 6 595.5 7 864.5 8 1100.5 9 1123.5 10 851.0 11 615.5 12 574.5 13 624.0 14 681.5 15 662.5 16 647.0 17 703.5 18 749.5 19 790.0 20 836.0 21 917.5 22 1096.5 23 1293.5 24 1447.5 25 1716.0 26 2204.5 27 2752.0 28 3129.0 29 3539.5 30 4165.0 31 4890.5 32 5917.0 33 6660.5 34 7158.0 35 7747.0 36 8482.0 37 9072.5 38 9087.5 39 9124.5 40 9199.5 41 9409.5 42 9616.5 43 9884.5 44 10338.0 45 10941.0 46 11272.0 47 11177.5 48 11323.0 49 11258.5 50 10991.0 51 10650.0 52 10391.5 53 10501.0 54 10627.5 55 10251.0 56 9392.0 57 8668.0 58 8205.5 59 7411.0 60 6218.0 61 5153.5 62 4256.0 63 3243.5 64 2311.5 65 1728.0 66 1246.0 67 994.0 68 832.5 69 626.0 70 486.0 71 361.0 72 299.5 73 286.5 74 224.5 75 162.0 76 116.5 77 73.5 78 51.0 79 30.0 80 13.5 81 11.0 82 7.5 83 1.0 84 1.0 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26498742054099117 2 0.018757536510204995 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 8.932160242954758E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003423994759799324 26-27 0.0026796480728864275 28-29 0.00208417072335611 30-31 0.0013398240364432138 32-33 0.0023819093981212692 34-35 0.0023819093981212692 36-37 0.0 38-39 0.0 40-41 4.466080121477379E-4 42-43 0.0047638187962425384 44-45 0.0032751254224167446 46-47 0.01488693373825793 48-49 0.01563128042517083 50-51 0.007145728194363807 52-53 0.0086344215681896 54-55 1.4886933738257933E-4 56-57 0.0035728640971819034 58-59 0.001042085361678055 60-61 0.0013398240364432138 62-63 0.0 64-65 7.443466869128966E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 5.954773495303173E-4 74-75 0.0 76-77 0.19531657064594407 78-79 0.10197549610706683 80-81 0.0014886933738257932 82-83 0.0 84-85 0.001042085361678055 86-87 0.11835112321915056 88-89 0.5655546127164188 90-91 0.3016092775371057 92-93 0.28359608771381356 94-95 0.0016375627112083723 96-97 0.0031262560850341655 98-99 0.0037217334345644826 100-101 8.932160242954758E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 335865.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.1639307985036 #Duplication Level Percentage of deduplicated Percentage of total 1 86.13882398275882 61.29977308973531 2 8.768925712818215 12.480624452084287 3 2.127491067912295 4.542018813940353 4 0.9503172780200171 2.705132520385552 5 0.516496221246212 1.837795067322702 6 0.332324615457879 1.4189715562250294 7 0.21664770263649033 1.079225148265459 8 0.1642085611845274 0.9348581347726043 9 0.128317989528425 0.8218451274303552 >10 0.6124941943936979 7.587442258418048 >50 0.02762739511304751 1.3268020829221454 >100 0.014232294452125815 1.7700624105750997 >500 8.371937913015184E-4 0.5347125592363352 >1k 0.0012557906869522777 1.6607367786866678 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2287 0.6809283491879178 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1902 0.5662989594033316 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1386 0.4126658032245098 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 930 0.2768969675315975 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 865 0.25754395367186217 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 344 0.10242210411921457 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.488693373825793E-4 0.0 12-13 0.0 0.0 0.0 2.977386747651586E-4 5.954773495303172E-4 14-15 0.0 0.0 0.0 2.977386747651586E-4 5.954773495303172E-4 16-17 0.0 0.0 0.0 2.977386747651586E-4 5.954773495303172E-4 18-19 0.0 0.0 0.0 2.977386747651586E-4 5.954773495303172E-4 20-21 0.0 0.0 0.0 4.466080121477379E-4 5.954773495303172E-4 22-23 0.0 0.0 0.0 8.932160242954758E-4 5.954773495303172E-4 24-25 0.0 0.0 0.0 0.0011909546990606344 5.954773495303172E-4 26-27 0.0 0.0 0.0 0.0013398240364432138 5.954773495303172E-4 28-29 0.0 0.0 0.0 0.0025307787355038484 5.954773495303172E-4 30-31 0.0 0.0 0.0 0.004614949458859959 5.954773495303172E-4 32-33 0.0 0.0 0.0 0.0064013815074509105 5.954773495303172E-4 34-35 0.0 0.0 0.0 0.009229898917719918 5.954773495303172E-4 36-37 0.0 0.0 0.0 0.015482411087788247 5.954773495303172E-4 38-39 0.0 0.0 0.0 0.027391958078394594 5.954773495303172E-4 40-41 0.0 0.0 0.0 0.037961681032557724 5.954773495303172E-4 42-43 0.0 0.0 0.0 0.04778705729980796 5.954773495303172E-4 44-45 0.0 0.0 0.0 0.05954773495303172 5.954773495303172E-4 46-47 0.0 0.0 0.0 0.07726318610155866 5.954773495303172E-4 48-49 0.0 0.0 0.0 0.09378768255102496 5.954773495303172E-4 50-51 0.0 0.0 0.0 0.11537373647149896 5.954773495303172E-4 52-53 0.0 0.0 0.0 0.13383353430693878 5.954773495303172E-4 54-55 0.0 0.0 0.0 0.16048114569842048 5.954773495303172E-4 56-57 2.977386747651586E-4 0.0 0.0 0.19099935986184924 5.954773495303172E-4 58-59 2.977386747651586E-4 0.0 0.0 0.22345287541125153 5.954773495303172E-4 60-61 2.977386747651586E-4 0.0 0.0 0.26305211915501764 5.954773495303172E-4 62-63 2.977386747651586E-4 0.0 0.0 0.30533101097167015 5.954773495303172E-4 64-65 2.977386747651586E-4 0.0 0.0 0.35564884700698196 5.954773495303172E-4 66-67 2.977386747651586E-4 0.0 0.0 0.4062644217170589 7.443466869128965E-4 68-69 2.977386747651586E-4 0.0 0.0 0.46715198070653385 8.932160242954758E-4 70-71 2.977386747651586E-4 0.0 0.0 0.5265508463221831 8.932160242954758E-4 72-73 2.977386747651586E-4 0.0 0.0 0.5898203147097792 8.932160242954758E-4 74-75 2.977386747651586E-4 0.0 0.0 0.6536852604469057 8.932160242954758E-4 76-77 2.977386747651586E-4 0.0 0.0 0.7241004570288657 8.932160242954758E-4 78-79 2.977386747651586E-4 0.0 0.0 0.8108912807229095 8.932160242954758E-4 80-81 5.954773495303172E-4 0.0 0.0 0.91018712875709 8.932160242954758E-4 82-83 5.954773495303172E-4 0.0 0.0 1.011716016852009 8.932160242954758E-4 84-85 5.954773495303172E-4 0.0 0.0 1.117115507718875 8.932160242954758E-4 86-87 5.954773495303172E-4 0.0 0.0 1.2412725350959462 0.001042085361678055 88-89 5.954773495303172E-4 0.0 0.0 1.3813585815729534 0.0011909546990606344 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 500 0.0 56.010197 2 GTACACG 70 1.2641976E-9 54.409233 1 GTACCTG 585 0.0 48.014988 1 ACACGGG 80 4.2527972E-9 47.44506 3 GGTATCA 965 0.0 42.921276 1 GTATCAA 1680 0.0 39.10664 1 TAACGCA 50 0.0016233902 37.95605 4 GTACATG 2160 0.0 33.942802 1 TACATGG 2185 0.0 33.454483 2 TATCAAC 2035 0.0 31.955177 2 ATCAACG 2020 0.0 31.943213 3 TCAACGC 2020 0.0 31.943213 4 TACACGG 120 1.5098158E-7 31.644176 2 CAACGCA 2045 0.0 31.55271 5 CATGGGA 1100 0.0 31.486269 4 ACATGGG 2200 0.0 31.486269 3 AACGCAG 2115 0.0 30.957064 6 ACCTGGG 885 0.0 28.413431 3 ATGGGAT 470 0.0 28.265144 5 ACGCAGA 2410 0.0 26.970846 7 >>END_MODULE