FastQCFastQC Report
Thu 26 May 2016
SRR936371_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936371_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634280
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41500.25393445431627387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29420.18001811195144038No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19530.11950216609148982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23400.048.1678961
GTATCAA38750.038.131111
GTACATG66350.030.76331
TCAACGC48200.030.6430034
ATCAACG48750.030.2991433
TACATGG67450.029.552242
CAACGCA50800.029.0746615
TATCAAC51500.028.959672
ACATGGG67950.028.4960213
AACGCAG52300.027.968386
GAGTACA48150.024.5889681
AGTACAT44900.023.9370022
ACGCAGA61150.023.8432147
CGCAGAG63150.023.0887898
GTGGTAT12400.022.9153331
CATGGGG40100.022.7843534
GTATAGG5850.022.6672441
CATGGGA26950.021.2544754
CATGGGT12550.020.3691944
GCAGAGT71050.020.3216769
Produced by FastQC (version 0.11.5)