##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936371_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1634280 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.520443253298087 33.0 31.0 34.0 30.0 34.0 2 31.60890606260861 33.0 31.0 34.0 30.0 34.0 3 31.67893690187728 34.0 31.0 34.0 30.0 34.0 4 35.1689673740118 37.0 35.0 37.0 33.0 37.0 5 35.14988435274249 37.0 35.0 37.0 33.0 37.0 6 35.23754252637247 37.0 35.0 37.0 33.0 37.0 7 35.1832280882101 37.0 35.0 37.0 32.0 37.0 8 35.18476026139952 37.0 35.0 37.0 32.0 37.0 9 36.82340786156595 39.0 37.0 39.0 33.0 39.0 10-11 36.73146798590204 39.0 37.0 39.0 33.0 39.0 12-13 36.62671818782583 39.0 37.0 39.0 32.0 39.0 14-15 37.83479575103409 40.0 38.0 41.0 32.5 41.0 16-17 37.776457828523874 40.0 38.0 41.0 32.0 41.0 18-19 37.721027914433265 40.0 38.0 41.0 32.0 41.0 20-21 37.63681407102822 40.0 38.0 41.0 32.0 41.0 22-23 37.46783629488215 40.0 37.5 41.0 32.0 41.0 24-25 37.63280037692439 40.0 38.0 41.0 32.0 41.0 26-27 37.58247148591428 40.0 38.0 41.0 32.0 41.0 28-29 37.441808625204985 40.0 38.0 41.0 32.0 41.0 30-31 37.27936032993122 40.0 37.0 41.0 31.0 41.0 32-33 37.08735161661404 40.0 37.0 41.0 31.0 41.0 34-35 36.82253071689062 40.0 36.5 41.0 30.0 41.0 36-37 36.629061115598304 40.0 36.0 41.0 30.0 41.0 38-39 36.455996830408495 40.0 36.0 41.0 30.0 41.0 40-41 36.272366118413004 40.0 36.0 41.0 30.0 41.0 42-43 36.07884358861395 40.0 35.0 41.0 29.0 41.0 44-45 35.89345919915804 39.0 35.0 41.0 28.0 41.0 46-47 35.60956721002521 39.0 35.0 41.0 27.0 41.0 48-49 35.43233166899185 39.0 35.0 41.0 26.5 41.0 50-51 35.13409727831217 38.5 35.0 40.5 26.0 41.0 52-53 35.17533133857111 39.0 35.0 40.5 26.0 41.0 54-55 35.27015811244095 39.0 35.0 41.0 26.0 41.0 56-57 35.117948576743274 39.0 35.0 41.0 26.0 41.0 58-59 34.88901014514037 39.0 35.0 41.0 26.0 41.0 60-61 34.60239493844384 38.0 34.0 40.5 25.0 41.0 62-63 34.299920760212444 38.0 34.0 40.0 23.5 41.0 64-65 33.97450528673177 37.0 34.0 40.0 23.0 41.0 66-67 33.62014954597743 37.0 34.0 40.0 22.0 41.0 68-69 33.24263406515408 36.0 33.0 39.0 21.5 41.0 70-71 32.84503726411631 36.0 33.0 39.0 20.5 41.0 72-73 32.42902470812835 35.0 32.5 38.5 19.5 40.0 74-75 32.00509368039748 35.0 32.0 37.5 18.0 39.5 76-77 31.596342120077345 35.0 32.0 37.0 15.0 39.0 78-79 31.187689991923047 35.0 31.5 36.5 10.0 39.0 80-81 30.77566022958122 35.0 31.0 36.0 7.0 38.0 82-83 30.413395195437744 35.0 31.0 35.5 4.5 37.0 84-85 30.07527014954598 34.5 31.0 35.0 2.0 37.0 86-87 29.733030753604034 34.0 30.5 35.0 2.0 36.0 88-89 29.38890306434638 34.0 30.0 35.0 2.0 36.0 90-91 29.0211426438554 34.0 29.5 35.0 2.0 36.0 92-93 28.690907004919598 34.0 29.0 35.0 2.0 35.0 94-95 28.297273417039918 34.0 29.0 35.0 2.0 35.0 96-97 27.854044900506644 34.0 29.0 35.0 2.0 35.0 98-99 27.333695878307267 34.0 28.0 35.0 2.0 35.0 100-101 24.046901081821964 30.0 14.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.443168313951535 1101 2 0.4343949563965914 1101 3 0.5114313147530325 1101 4 0.37233416972746625 1101 5 0.5061090675789188 1101 6 0.3808477467056761 1101 7 0.47678890195765433 1101 8 0.5665703043942969 1101 9 0.6137013356000551 1101 10-11 0.770791540817271 1101 12-13 0.788879110266997 1101 14-15 0.8494706891254609 1101 16-17 0.8746032059578113 1101 18-19 0.7668355571206504 1101 20-21 0.8923508422571231 1101 22-23 0.8607634441397991 1101 24-25 0.931125750098154 1101 26-27 0.8404140443659003 1101 28-29 0.8378336240075512 1101 30-31 0.9883755953132791 1101 32-33 1.005645780442073 1101 34-35 0.7489331618261588 1101 36-37 0.6473235080824153 1101 38-39 0.8270902327321181 1101 40-41 0.8375046471939456 1101 42-43 0.6375466210177336 1101 44-45 0.5597824536080509 1101 46-47 0.6592821944974503 1101 48-49 0.7982302715518799 1101 50-51 0.7740755739663214 1101 52-53 0.8956838871057329 1101 54-55 0.8852189312268308 1101 56-57 0.8232759043299325 1101 58-59 0.8813001757633785 1101 60-61 0.7621654549599768 1101 62-63 0.9506628615402377 1101 64-65 0.9281486729010098 1101 66-67 1.0323624484235907 1101 68-69 0.8409828861416813 1101 70-71 0.8259229791581149 1101 72-73 0.9565009659361863 1101 74-75 0.8824961635540234 1101 76-77 0.8824132708838697 1101 78-79 0.9268754989210812 1101 80-81 0.8918646125500906 1101 82-83 1.059721352903928 1101 84-85 0.9316455511836637 1101 86-87 0.8025188083565347 1101 88-89 0.5444564094011355 1101 90-91 0.752488695064276 1101 92-93 0.8197295107796769 1101 94-95 1.013077536419491 1101 96-97 0.9965209259722307 1101 98-99 0.9889486842388671 1101 100-101 0.8083484896058906 1102 1 0.3711093491167148 1102 2 0.3511704091976391 1102 3 0.2340941275596755 1102 4 0.2319952034561794 1102 5 0.2968752357867075 1102 6 0.35635752095786444 1102 7 0.2557028006035935 1102 8 0.3417891950377836 1102 9 0.44370087942622405 1102 10-11 0.4459647170197556 1102 12-13 0.4328902758493598 1102 14-15 0.5208158875077018 1102 16-17 0.6186411596202532 1102 18-19 0.6188338411305949 1102 20-21 0.5224096401517286 1102 22-23 0.6140330943127097 1102 24-25 0.7297286449578451 1102 26-27 0.6480394751832534 1102 28-29 0.5957373208596621 1102 30-31 0.47383578249984026 1102 32-33 0.3240662737115656 1102 34-35 0.46363743986313466 1102 36-37 0.44689916936462026 1102 38-39 0.30155857184204393 1102 40-41 0.3509066945409032 1102 42-43 0.2464751075203253 1102 44-45 0.21236410629671099 1102 46-47 0.32436349534991393 1102 48-49 0.41853180245183097 1102 50-51 0.39582594205977273 1102 52-53 0.46047003378599527 1102 54-55 0.37397060669506743 1102 56-57 0.3676587059942875 1102 58-59 0.4797126423146736 1102 60-61 0.4555433639173785 1102 62-63 0.6224931085826313 1102 64-65 0.48719576459874503 1102 66-67 0.5208241623366021 1102 68-69 0.6028941185971846 1102 70-71 0.6300035996321682 1102 72-73 0.49492341978255894 1102 74-75 0.3822853044816412 1102 76-77 0.40223086929366403 1102 78-79 0.4157630559549368 1102 80-81 0.42804720437448296 1102 82-83 0.36881527521267543 1102 84-85 0.5760405821889236 1102 86-87 0.2585987319522687 1102 88-89 0.24979941814899576 1102 90-91 0.4165610752919555 1102 92-93 0.5725849648230756 1102 94-95 0.6414858585830352 1102 96-97 0.9145079085182424 1102 98-99 0.723352392252675 1102 100-101 0.47601056962265886 1103 1 0.2938301264270784 1103 2 0.16250338337262704 1103 3 0.22412652822755774 1103 4 0.3323637228450025 1103 5 0.23515922305568893 1103 6 0.20887048313543488 1103 7 0.2938824791186647 1103 8 0.1044972871810046 1103 9 0.14891880066356578 1103 10-11 0.1311853510488632 1103 12-13 0.14230538266075854 1103 14-15 0.23916314777905257 1103 16-17 0.3683393137755999 1103 18-19 0.1695625695954206 1103 20-21 0.25585635671848905 1103 22-23 0.2213240087527808 1103 24-25 0.2362202410346157 1103 26-27 0.39726957723771505 1103 28-29 0.3245699033992153 1103 30-31 0.205305695346631 1103 32-33 0.30092730617718644 1103 34-35 0.24723164060208092 1103 36-37 -0.001159942750099674 1103 38-39 0.10351092222774838 1103 40-41 0.10310606181087678 1103 42-43 0.18192690197476225 1103 44-45 0.2746861987379532 1103 46-47 0.2450305746666004 1103 48-49 0.3552296662238277 1103 50-51 0.32262784712734316 1103 52-53 0.3276449668259005 1103 54-55 0.23521035253322253 1103 56-57 0.3904246646605145 1103 58-59 0.40455445980874316 1103 60-61 0.30719998179200303 1103 62-63 0.3479886706845434 1103 64-65 0.19615332660131202 1103 66-67 0.21578696652269969 1103 68-69 0.21754825675088085 1103 70-71 0.2571657137757555 1103 72-73 0.3115983967620366 1103 74-75 0.3062091667329554 1103 76-77 0.07198047097177707 1103 78-79 0.24844493718050487 1103 80-81 0.2200343434490044 1103 82-83 0.31660358575473424 1103 84-85 0.4041771043898805 1103 86-87 0.38422821426754084 1103 88-89 0.2704792017369897 1103 90-91 0.48747622222211007 1103 92-93 0.3513677810065978 1103 94-95 0.36118044025908347 1103 96-97 0.462560143132702 1103 98-99 0.5693011625174194 1103 100-101 0.8017094000655334 1104 1 0.144307903954644 1104 2 0.011350411994239806 1104 3 0.10355619772410662 1104 4 0.10250694350840917 1104 5 0.1735848466435641 1104 6 0.09478186347336504 1104 7 0.09843844251783196 1104 8 0.02678619058212206 1104 9 0.04052560439571096 1104 10-11 0.13543674534855654 1104 12-13 0.24255234072292353 1104 14-15 0.3172767710066253 1104 16-17 0.36350524106934756 1104 18-19 0.25848717607637894 1104 20-21 0.3256848747449226 1104 22-23 0.3731234622923907 1104 24-25 0.3679487222800617 1104 26-27 0.33311677850478105 1104 28-29 0.50587953176111 1104 30-31 0.3818366101423507 1104 32-33 0.3888113484828253 1104 34-35 0.45861347980160616 1104 36-37 0.3605144554303692 1104 38-39 0.3666338532971807 1104 40-41 0.3528161300433652 1104 42-43 0.375778659580547 1104 44-45 0.19164092059111226 1104 46-47 0.19037327589238373 1104 48-49 0.36187088645187515 1104 50-51 0.32972413825261526 1104 52-53 0.1247471504155726 1104 54-55 0.24784818740910453 1104 56-57 0.25617781168688225 1104 58-59 0.2803004007639984 1104 60-61 0.24278727658786892 1104 62-63 0.23188741787905798 1104 64-65 0.32490461205931354 1104 66-67 0.46702550999557957 1104 68-69 0.3392989567156022 1104 70-71 0.36403794112729315 1104 72-73 0.2246995594916399 1104 74-75 0.15639795078351426 1104 76-77 0.33931444984898107 1104 78-79 0.3835700300712652 1104 80-81 0.3932446481306968 1104 82-83 0.3523918623777931 1104 84-85 0.11771701176184024 1104 86-87 0.12983607639138128 1104 88-89 0.17273560275484812 1104 90-91 0.35792269837827106 1104 92-93 0.009343062681406167 1104 94-95 -0.0768445332811254 1104 96-97 0.15674687045046554 1104 98-99 0.28801574205067837 1104 100-101 0.6726578616444137 1105 1 0.37032589522006987 1105 2 0.35339157943229793 1105 3 0.36198167193463604 1105 4 0.2812194549070455 1105 5 0.29678803155439226 1105 6 0.2941338407177909 1105 7 0.39087582026007794 1105 8 0.38048388148551027 1105 9 0.40282008899599475 1105 10-11 0.48850377205807405 1105 12-13 0.37822060455855677 1105 14-15 0.427898624484051 1105 16-17 0.4712203286054475 1105 18-19 0.6052373632587802 1105 20-21 0.555732499749233 1105 22-23 0.5325940306923798 1105 24-25 0.4550429262427116 1105 26-27 0.4777339927592905 1105 28-29 0.5411903814513792 1105 30-31 0.5132737812134636 1105 32-33 0.5286261784051831 1105 34-35 0.23133574643999566 1105 36-37 0.4152297296956391 1105 38-39 0.5485993954191102 1105 40-41 0.3492302047096558 1105 42-43 0.2952333469360866 1105 44-45 0.471046233489389 1105 46-47 0.2883571446670885 1105 48-49 0.29585736399632623 1105 50-51 0.18439535893568149 1105 52-53 0.3647560064546269 1105 54-55 0.640386226680242 1105 56-57 0.7174874811762422 1105 58-59 0.7237638371972182 1105 60-61 0.6801228688003604 1105 62-63 0.36138818020544505 1105 64-65 0.3703820042322761 1105 66-67 0.40893098152300666 1105 68-69 0.41557850479473757 1105 70-71 0.47251875425127565 1105 72-73 0.5667155728781239 1105 74-75 0.43835877935345025 1105 76-77 0.578748615124244 1105 78-79 0.5363086037866864 1105 80-81 0.5042537833570648 1105 82-83 0.5090685181489008 1105 84-85 0.6139785758271685 1105 86-87 0.5283428775030536 1105 88-89 0.5518386591307447 1105 90-91 0.4041257056244483 1105 92-93 0.3117301141914659 1105 94-95 0.3004870886702484 1105 96-97 0.3225639646906906 1105 98-99 0.22287830575161038 1105 100-101 0.4942272569138204 1106 1 0.07198202124365949 1106 2 0.10982080676035366 1106 3 0.1189345634518375 1106 4 0.15952120107724 1106 5 0.067584779013103 1106 6 0.015570132080199528 1106 7 -0.011842366271281435 1106 8 0.02541044464484088 1106 9 0.042061527738361804 1106 10-11 0.09336459254642904 1106 12-13 0.09339291615135181 1106 14-15 0.12189882351989922 1106 16-17 0.13725599078858863 1106 18-19 0.11544207901776815 1106 20-21 0.30156393724436015 1106 22-23 0.15727217277529348 1106 24-25 0.06398050503678832 1106 26-27 0.13510724972891097 1106 28-29 0.07970707764749108 1106 30-31 0.21135334601158462 1106 32-33 0.18793286440179457 1106 34-35 0.11767476011740285 1106 36-37 0.1434188989295606 1106 38-39 0.23050686287044897 1106 40-41 0.06574796387888426 1106 42-43 0.2130697837945661 1106 44-45 0.24899110577399597 1106 46-47 0.3643677622301311 1106 48-49 0.4042660636881763 1106 50-51 0.39845537302463896 1106 52-53 0.3673467318186141 1106 54-55 0.4336564247991461 1106 56-57 0.4330081055056354 1106 58-59 0.43349829816858687 1106 60-61 0.5796701708868 1106 62-63 0.4407387323126457 1106 64-65 0.32629882289193546 1106 66-67 0.38820843793175897 1106 68-69 0.4077291914466912 1106 70-71 0.453511482489553 1106 72-73 0.5819759295868678 1106 74-75 0.4131692281271384 1106 76-77 0.4716888396175598 1106 78-79 0.4925961158516081 1106 80-81 0.6200402813386781 1106 82-83 0.5347387105313892 1106 84-85 0.44723107800765405 1106 86-87 0.2881772257888464 1106 88-89 0.15864377304834676 1106 90-91 0.17536072307050787 1106 92-93 0.2041686205882094 1106 94-95 0.47327497686077535 1106 96-97 0.5498896312326309 1106 98-99 0.2840818294683878 1106 100-101 0.17185697461754046 1107 1 0.006888903656978584 1107 2 0.18725047229083813 1107 3 0.2608883403904372 1107 4 0.11362746801248846 1107 5 0.1599398791197686 1107 6 0.07317240225018651 1107 7 0.11731929186029078 1107 8 0.11893694734258986 1107 9 0.18966237081890114 1107 10-11 0.20568705255590913 1107 12-13 0.13233073164332865 1107 14-15 0.15530026378247896 1107 16-17 -0.05838013433422162 1107 18-19 0.15026146072212043 1107 20-21 0.33953114894942615 1107 22-23 0.1771976805355493 1107 24-25 0.30024957177518274 1107 26-27 0.28435953718863516 1107 28-29 0.11816668095320892 1107 30-31 0.1465024219711637 1107 32-33 0.26554869192156616 1107 34-35 0.10440054122017983 1107 36-37 0.25503261691839185 1107 38-39 0.1963703800604648 1107 40-41 0.2893024411890792 1107 42-43 -0.10806719483751692 1107 44-45 0.1711257663005199 1107 46-47 0.2288228519080704 1107 48-49 0.11984873699262977 1107 50-51 0.16865303406162724 1107 52-53 0.11460644563229039 1107 54-55 0.20049150631705714 1107 56-57 0.18987254298539824 1107 58-59 0.4021787161228403 1107 60-61 0.5758181728700933 1107 62-63 0.4809753364252245 1107 64-65 0.3066152799777129 1107 66-67 0.3469303329601914 1107 68-69 0.3974536899985637 1107 70-71 0.34814425395214954 1107 72-73 0.3668163517126146 1107 74-75 0.42097408096311284 1107 76-77 0.42760412539222514 1107 78-79 0.20149433875931777 1107 80-81 0.16477587642583202 1107 82-83 0.2575659211020991 1107 84-85 0.30248601473087433 1107 86-87 -0.020588671164315286 1107 88-89 0.15299419120385238 1107 90-91 0.4162415299981106 1107 92-93 0.10794231164529933 1107 94-95 0.0024836557601624065 1107 96-97 0.266412262492846 1107 98-99 0.290688064385602 1107 100-101 0.16299895721070357 1108 1 -0.20008631067117832 1108 2 -0.12181709412966768 1108 3 -0.057266952029561224 1108 4 -0.10926850855995696 1108 5 -0.07906254002971025 1108 6 -0.032750269848953906 1108 7 -0.12737555577572834 1108 8 -0.045066532837360285 1108 9 -0.038581199336434224 1108 10-11 -0.12174164619631966 1108 12-13 -0.1755033868525615 1108 14-15 -0.22554070897025014 1108 16-17 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64-65 -1.6902334595180903 2316 66-67 -1.6680089760967647 2316 68-69 -1.818232584511243 2316 70-71 -1.6299136358704445 2316 72-73 -1.5943181020689003 2316 74-75 -1.5443620534906692 2316 76-77 -1.7164201509849946 2316 78-79 -1.7437670991491778 2316 80-81 -1.5492764111558692 2316 82-83 -1.617784218953922 2316 84-85 -1.628536394232711 2316 86-87 -1.7507474013230464 2316 88-89 -1.6816229890015642 2316 90-91 -1.7714335400299 2316 92-93 -1.7224731878878465 2316 94-95 -1.8467226934461891 2316 96-97 -2.204526112857298 2316 98-99 -1.7812073604509955 2316 100-101 -1.7970850764027375 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18176.0 3 3768.0 4 3107.0 5 2524.0 6 2419.0 7 2577.0 8 3182.0 9 3687.0 10 4273.0 11 5087.0 12 6395.0 13 8826.0 14 9053.0 15 8376.0 16 9650.0 17 11837.0 18 10875.0 19 8231.0 20 8134.0 21 8246.0 22 8821.0 23 9766.0 24 11042.0 25 12845.0 26 14974.0 27 18063.0 28 21233.0 29 25710.0 30 31154.0 31 38436.0 32 48093.0 33 63189.0 34 86208.0 35 127132.0 36 206487.0 37 342867.0 38 356623.0 39 73084.0 40 130.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.18837570337866 18.210406331265027 12.652429319320447 23.948788646035858 2 15.807104430738011 19.130693400126187 40.51693065386783 24.54527151526797 3 20.192114693585815 23.472702260777613 28.057288045979263 28.27789499965731 4 12.62591000016522 15.112137231421066 36.603530895488504 35.65842187292521 5 13.900073676815792 37.51288120408967 34.04786790065134 14.539177218443191 6 34.011036619842415 35.049406558332045 16.843144885925557 14.096411935899978 7 30.37902569468666 31.371076802574976 19.852893482398006 18.39700402034035 8 26.937554118845753 35.88656995903031 18.948006131684348 18.22786979043959 9 26.9407418241636 16.015943179321603 19.567051986685865 37.47626300982893 10-11 25.956326116766782 26.230794695300407 26.696084868340137 21.116794319592668 12-13 26.18942641642676 23.18022615586133 27.622352695319275 23.007994732392632 14-15 22.8890993288529 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22.510800823803887 40-41 22.967855933000557 26.4327973632065 28.005141034145886 22.594205669647057 42-43 22.85392857801521 26.402220487253096 27.825988061499874 22.91786287323182 44-45 22.945314339714752 26.575664287853044 27.81594905686711 22.66307231556509 46-47 22.9357392011717 26.940579466869817 27.451629623754194 22.67205170820429 48-49 23.170807447687064 26.76124369167082 27.428095654777973 22.639853205864142 50-51 22.959266506855126 26.969611618602258 27.325743720181393 22.74537815436122 52-53 22.97208051908442 27.055652028472903 27.187028054761637 22.785239397681043 54-55 23.012809556073933 26.820323134793618 27.367745053312692 22.79912225581976 56-57 22.885362311044165 27.093688279550886 27.32832781291547 22.692621596489477 58-59 23.016323134179476 26.869524667603002 27.335293916934333 22.778858281283192 60-61 23.09003667124673 26.90848787205331 27.471989376711758 22.529486079988196 62-63 22.933955553970836 27.060019783026068 27.357871744760846 22.648152918242246 64-65 22.885529619801133 26.867577219056425 27.36320809241447 22.883685068727974 66-67 22.87666231047171 27.06783001250306 27.205031712525557 22.850475964499676 68-69 22.676955238563696 26.934394982784067 27.397165518937534 22.991484259714706 70-71 22.758275582888615 26.78066347284628 27.466107409987075 22.994953534278036 72-73 22.866820653025943 27.059280649669148 27.27891716890525 22.794981528399664 74-75 22.876442119385935 27.01930532318293 27.087587520191054 23.016665037240085 76-77 22.888138556776074 27.106666363364173 27.087084908508185 22.918110171351564 78-79 22.890107772076153 27.006097933981728 27.172187248410655 22.931607045531464 80-81 22.657214491940998 27.20291496362751 27.157695277724493 22.982175266706996 82-83 23.013390544693507 27.326376197483714 26.92548064582272 22.73475261200006 84-85 23.003396224559054 27.048555436418543 26.8766538457755 23.0713944932469 86-87 22.763136699387758 27.079529091765377 27.183096686451798 22.97423752239507 88-89 23.00744560911367 27.02669713738974 27.056950593093788 22.90890666040281 90-91 22.955499053446157 27.247311946997904 26.977860849819606 22.819328149736332 92-93 22.852515594279975 27.159107471540402 27.09628756817789 22.892089366001734 94-95 22.789769455860252 27.18264099183442 27.148488353827478 22.879101198477855 96-97 22.948666885482087 27.125336981896098 26.975423831949758 22.95057230067206 98-99 22.83662400319592 26.907487362291228 26.99190264738872 23.263985987124133 100-101 22.91914318202772 27.15298564799164 26.642582746857617 23.28528842312302 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 144.0 1 108.5 2 180.0 3 302.5 4 251.5 5 220.5 6 423.5 7 837.0 8 1256.0 9 1430.5 10 1309.0 11 1225.0 12 1303.0 13 1478.0 14 1671.0 15 1793.5 16 1946.5 17 2036.0 18 2100.5 19 2238.0 20 2427.5 21 2660.5 22 2996.5 23 3592.5 24 4408.0 25 5491.5 26 7017.5 27 9024.0 28 10974.0 29 13134.0 30 16216.0 31 19357.0 32 22505.0 33 26031.0 34 29835.0 35 33286.5 36 37698.0 37 42241.5 38 44681.0 39 46802.0 40 48616.5 41 50829.5 42 52802.5 43 54525.0 44 57091.0 45 59472.5 46 60971.0 47 61374.5 48 61654.5 49 61088.0 50 58826.0 51 57028.5 52 56891.5 53 56335.5 54 55276.0 55 52153.0 56 47136.0 57 43241.5 58 40508.5 59 36655.0 60 31728.0 61 26717.0 62 21679.0 63 17749.5 64 13641.0 65 10043.5 66 8171.5 67 6720.0 68 5199.5 69 3920.0 70 3008.5 71 2373.5 72 1845.5 73 1619.5 74 1351.5 75 975.5 76 750.0 77 499.0 78 314.5 79 223.0 80 174.5 81 132.0 82 90.0 83 57.0 84 36.5 85 21.0 86 17.0 87 17.5 88 21.5 89 22.5 90 15.5 91 8.5 92 10.0 93 13.5 94 14.0 95 14.5 96 13.0 97 11.5 98 9.0 99 6.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04503512250042833 2 0.010585701348606114 3 0.009300731820740631 4 0.004711554935506767 5 0.007097926915828377 6 0.005139878111461928 7 0.005507012262280637 8 0.00887240864478547 9 0.013889908705974497 10-11 0.0018968597792299975 12-13 0.00865824705680789 14-15 0.008413490956262084 16-17 0.017867195339843846 18-19 0.010555106836037888 20-21 0.0033653963825048343 22-23 0.009484298896149986 24-25 0.020192378295029005 26-27 0.006547225689600313 28-29 0.016765792887387717 30-31 0.013033262354064174 32-33 0.01141175318794821 34-35 0.25800352448784786 36-37 0.45793866412120315 38-39 0.45503218542722174 40-41 0.4747656460337274 42-43 0.46564848128839614 44-45 0.47966076804464347 46-47 0.4848006461561054 48-49 0.49299997552438996 50-51 0.5016276280686296 52-53 0.49147024989597865 54-55 0.47959957901950706 56-57 0.4737560271189759 58-59 0.47259343564138334 60-61 0.46901387767090097 62-63 0.4670558288665345 64-65 0.48143524977360064 66-67 0.4575715299703845 68-69 0.482169518075238 70-71 0.46485302396162226 72-73 0.4729299752796339 74-75 0.48590204860856157 76-77 0.47366424358127124 78-79 0.4739395941943853 80-81 0.4758058594610471 82-83 0.4770602344763443 84-85 0.4753163472599554 86-87 0.4776415302151406 88-89 0.490705387081773 90-91 0.5128864086937367 92-93 0.5019335731943119 94-95 0.47473505152115913 96-97 0.44900506645128124 98-99 0.4408669261081332 100-101 0.4485155542501897 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1634280.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.96821733668064 #Duplication Level Percentage of deduplicated Percentage of total 1 76.2790078162206 43.454790955008164 2 13.376048855191819 15.240193165772517 3 4.493512577857224 7.679622034214353 4 1.9980801524591763 4.553082575256093 5 1.0257525775794287 2.9217647886602633 6 0.6143694625252174 2.0999719839693753 7 0.4157725951163379 1.6580076492856284 8 0.29676233333543084 1.3524816882234634 9 0.20959647458292854 1.07463037653381 >10 1.1618161916107645 12.277589728588428 >50 0.093768240216391 3.6611673718699707 >100 0.034544293230968325 3.2106760035537896 >500 6.456200491189026E-4 0.2615658431915907 >1k 3.228100245594513E-4 0.5544558358724954 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4150 0.25393445431627387 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2942 0.18001811195144038 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1953 0.11950216609148982 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.0594512568225764E-5 0.0 16-17 0.0 0.0 0.0 1.2237805027290305E-4 0.0 18-19 0.0 0.0 0.0 1.8356707540935458E-4 0.0 20-21 0.0 0.0 0.0 2.1416158797758035E-4 0.0 22-23 0.0 0.0 0.0 5.507012262280637E-4 0.0 24-25 0.0 0.0 0.0 7.648628142056441E-4 0.0 26-27 0.0 0.0 0.0 9.484298896149986E-4 0.0 28-29 0.0 0.0 0.0 0.0011931859901608047 0.0 30-31 0.0 0.0 0.0 0.002233399417480481 0.0 32-33 0.0 0.0 0.0 0.005109283598893703 0.0 34-35 0.0 0.0 0.0 0.007618033629488215 0.0 36-37 0.0 0.0 0.0 0.012849695278654821 0.0 38-39 0.0 0.0 0.0 0.02361896370267029 0.0 40-41 0.0 0.0 0.0 0.034908338840345596 0.0 42-43 0.0 0.0 0.0 0.04677900971681719 0.0 44-45 0.0 0.0 0.0 0.060791296473064596 0.0 46-47 0.0 0.0 0.0 0.07572141860635877 0.0 48-49 0.0 0.0 0.0 0.09450644932324939 0.0 50-51 0.0 0.0 0.0 0.11665687642264483 0.0 52-53 0.0 0.0 0.0 0.13902146511001787 0.0 54-55 0.0 0.0 0.0 0.1624262672247106 0.0 56-57 0.0 0.0 0.0 0.18996132853611375 0.0 58-59 0.0 0.0 0.0 0.21966860023986096 0.0 60-61 0.0 0.0 0.0 0.2576669848495974 0.0 62-63 0.0 0.0 0.0 0.29480872310742345 0.0 64-65 0.0 0.0 0.0 0.33608072056196003 0.0 66-67 0.0 0.0 0.0 0.3811158430623883 0.0 68-69 0.0 0.0 0.0 0.43496218518246565 0.0 70-71 0.0 0.0 0.0 0.49492742981618815 0.0 72-73 0.0 0.0 0.0 0.5580133147318698 0.0 74-75 0.0 0.0 0.0 0.6229960594267812 0.0 76-77 0.0 0.0 0.0 0.6977078591183885 0.0 78-79 0.0 0.0 0.0 0.7852387595760824 0.0 80-81 0.0 0.0 0.0 0.8872408644785472 0.0 82-83 0.0 0.0 0.0 0.9893347529187164 0.0 84-85 0.0 0.0 0.0 1.092101720635387 0.0 86-87 0.0 0.0 0.0 1.2053320116503903 0.0 88-89 0.0 0.0 0.0 1.3346550162762807 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2340 0.0 48.167896 1 GTATCAA 3875 0.0 38.13111 1 GTACATG 6635 0.0 30.7633 1 TCAACGC 4820 0.0 30.643003 4 ATCAACG 4875 0.0 30.299143 3 TACATGG 6745 0.0 29.55224 2 CAACGCA 5080 0.0 29.074661 5 TATCAAC 5150 0.0 28.95967 2 ACATGGG 6795 0.0 28.496021 3 AACGCAG 5230 0.0 27.96838 6 GAGTACA 4815 0.0 24.588968 1 AGTACAT 4490 0.0 23.937002 2 ACGCAGA 6115 0.0 23.843214 7 CGCAGAG 6315 0.0 23.088789 8 GTGGTAT 1240 0.0 22.915333 1 CATGGGG 4010 0.0 22.784353 4 GTATAGG 585 0.0 22.667244 1 CATGGGA 2695 0.0 21.254475 4 CATGGGT 1255 0.0 20.369194 4 GCAGAGT 7105 0.0 20.321676 9 >>END_MODULE