FastQCFastQC Report
Thu 26 May 2016
SRR936371_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936371_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634280
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41040.25111975915999707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32190.19696747191423747No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22850.13981692243679172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26750.048.3705861
GTATCAA46000.040.1245461
TCAACGC56050.031.9969234
ATCAACG56900.031.6023243
CAACGCA57750.031.0550235
TATCAAC59950.030.0791952
AACGCAG59850.030.0446476
TACATGG63100.026.321492
GTACATG64400.026.1489121
ACGCAGA70050.025.6698387
CGCAGAG72150.024.922698
GTATAGG5550.024.8565731
ACATGGG64600.024.6773663
GCAGAGT81350.021.9291769
CGTTCGG550.002897842821.56598934-35
GTGGTAT15650.020.9735031
CATGGGG34850.020.9657294
GAGTACA51150.019.9953521
AGTACAT47000.019.8902442
TTACGCG600.004770180719.78819780-81
Produced by FastQC (version 0.11.5)