##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936371_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1634280 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45770002692317 34.0 31.0 34.0 31.0 34.0 2 32.59553075360403 34.0 31.0 34.0 31.0 34.0 3 32.67664537288592 34.0 31.0 34.0 31.0 34.0 4 36.16161857209291 37.0 35.0 37.0 35.0 37.0 5 36.124837237193134 37.0 35.0 37.0 35.0 37.0 6 36.16673948160658 37.0 36.0 37.0 35.0 37.0 7 36.117935727048 37.0 36.0 37.0 35.0 37.0 8 36.09851555425019 37.0 36.0 37.0 35.0 37.0 9 37.86358273979979 39.0 38.0 39.0 35.0 39.0 10-11 37.79674260224686 39.0 38.0 39.0 35.0 39.0 12-13 37.74149013632915 39.0 38.0 39.0 35.0 39.0 14-15 39.08230994688793 40.0 38.0 41.0 36.0 41.0 16-17 39.00603568543946 40.0 38.0 41.0 35.5 41.0 18-19 39.02636971632768 40.0 38.5 41.0 35.0 41.0 20-21 38.98057768558631 40.0 38.5 41.0 35.0 41.0 22-23 38.86285948552268 40.0 38.0 41.0 35.0 41.0 24-25 39.02423238367966 40.0 39.0 41.0 35.0 41.0 26-27 38.94238686149252 40.0 38.0 41.0 35.0 41.0 28-29 38.84665265437991 40.0 38.0 41.0 35.0 41.0 30-31 38.73356891108011 40.0 38.0 41.0 35.0 41.0 32-33 38.57842658540764 40.0 38.0 41.0 34.0 41.0 34-35 38.44261570844653 40.0 38.0 41.0 34.0 41.0 36-37 38.303869288004506 40.0 38.0 41.0 34.0 41.0 38-39 38.163006645128135 40.0 38.0 41.0 33.5 41.0 40-41 38.024200871331715 40.0 38.0 41.0 33.0 41.0 42-43 38.04742853121864 40.0 38.0 41.0 33.0 41.0 44-45 37.915846121839586 40.0 38.0 41.0 33.0 41.0 46-47 37.72028232616198 40.0 37.5 41.0 33.0 41.0 48-49 37.49293052598086 40.0 37.0 41.0 32.5 41.0 50-51 37.29819002863646 40.0 37.0 41.0 32.0 41.0 52-53 37.10370866681352 40.0 36.5 41.0 31.5 41.0 54-55 36.92146847541425 40.0 36.0 41.0 31.0 41.0 56-57 36.67527382088748 39.0 35.5 41.0 31.0 41.0 58-59 36.42405340578114 39.0 35.0 41.0 31.0 41.0 60-61 36.15204922045182 39.0 35.0 41.0 30.0 41.0 62-63 35.85918997968524 38.0 35.0 40.0 30.0 41.0 64-65 35.48568666324008 37.5 35.0 40.0 29.0 41.0 66-67 35.138878894681454 37.0 34.0 40.0 29.0 41.0 68-69 34.78488784051693 36.5 34.0 39.0 28.0 41.0 70-71 34.414792140881616 36.0 34.0 39.0 28.0 41.0 72-73 34.00262042000147 35.5 34.0 38.5 27.5 40.0 74-75 33.56488790170595 35.0 33.5 37.5 26.5 39.5 76-77 32.83895599285312 35.0 32.5 37.0 26.0 39.0 78-79 32.885742651198086 35.0 33.0 36.5 26.0 39.0 80-81 32.70295726558484 35.0 33.0 36.0 26.0 38.5 82-83 32.39821481019165 35.0 33.0 36.0 26.0 37.0 84-85 32.09060197762929 35.0 33.0 35.0 26.0 37.0 86-87 31.668230964094278 35.0 32.0 35.0 25.0 36.5 88-89 31.169224673862495 35.0 32.0 35.0 23.5 36.0 90-91 30.93559977482439 34.0 32.0 35.0 21.5 36.0 92-93 30.627723217563698 34.0 31.0 35.0 20.0 35.5 94-95 30.45092609589544 34.0 31.0 35.0 19.0 35.0 96-97 30.17775931908853 34.0 31.0 35.0 14.5 35.0 98-99 29.695926034706417 34.0 31.0 35.0 2.0 35.0 100-101 26.301245196661526 31.0 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10944340324573432 1101 2 0.1218159261973284 1101 3 0.23308304204123687 1101 4 0.23514809231415512 1101 5 0.20667544746451938 1101 6 0.2515955222912041 1101 7 0.23236314783866163 1101 8 0.12905752585664487 1101 9 0.1768060011405126 1101 10-11 0.25302334293301243 1101 12-13 0.33082696971909087 1101 14-15 0.5303249153265241 1101 16-17 0.5446539144242593 1101 18-19 0.4333538767449525 1101 20-21 0.3013367522958461 1101 22-23 0.3891144764698282 1101 24-25 0.30015105205318804 1101 26-27 0.32044311984373763 1101 28-29 0.44513515948265336 1101 30-31 0.372362863466293 1101 32-33 0.45209089692894366 1101 34-35 0.45352355860210025 1101 36-37 0.29430860525947367 1101 38-39 0.3098357792978703 1101 40-41 0.42698629271243504 1101 42-43 0.28949337604026937 1101 44-45 0.36891493244021234 1101 46-47 0.3767802126236717 1101 48-49 0.28959562965303576 1101 50-51 0.2628107072870236 1101 52-53 0.3206267908100031 1101 54-55 0.2935293131499179 1101 56-57 0.2818950448742612 1101 58-59 0.15492818392235108 1101 60-61 0.2344604745488823 1101 62-63 0.206153106142267 1101 64-65 0.3274635115640905 1101 66-67 0.3004958810461531 1101 68-69 0.348974757129497 1101 70-71 0.33865336579425787 1101 72-73 0.31416097698836865 1101 74-75 0.27120175448268924 1101 76-77 0.14275889470866332 1101 78-79 0.2139921525684727 1101 80-81 0.4210949627879046 1101 82-83 0.386859039921184 1101 84-85 0.49480429561777584 1101 86-87 0.32312720553244034 1101 88-89 0.3099003399126623 1101 90-91 0.5174761007935764 1101 92-93 0.5289554469148179 1101 94-95 0.5630471246824591 1101 96-97 0.47624432952373397 1101 98-99 0.4368872497579446 1101 100-101 0.8942104296957076 1102 1 0.025181788526261073 1102 2 0.1075949497718085 1102 3 0.04513748396109207 1102 4 0.12121916419647505 1102 5 0.09610716163958699 1102 6 0.15477650974253976 1102 7 0.15626704463079477 1102 8 0.15327744434711832 1102 9 0.1908517874046325 1102 10-11 0.13626274004591465 1102 12-13 0.20729849428114022 1102 14-15 0.2725034753507458 1102 16-17 0.3075333030348588 1102 18-19 0.343097458524781 1102 20-21 0.18487727266401066 1102 22-23 0.21155949053841994 1102 24-25 0.20898131559548005 1102 26-27 0.20689320423589663 1102 28-29 0.20867502956046735 1102 30-31 0.13959822683786172 1102 32-33 0.12644126172241954 1102 34-35 0.16131947225519383 1102 36-37 0.08180633652261804 1102 38-39 0.21253099015208932 1102 40-41 0.13295678311871484 1102 42-43 0.3120608776344511 1102 44-45 0.11788538693493678 1102 46-47 0.27869920003424653 1102 48-49 0.35012528629002304 1102 50-51 0.06901690368052726 1102 52-53 0.09505659779024 1102 54-55 0.21309033804633515 1102 56-57 0.35533490611977214 1102 58-59 0.09475230464729378 1102 60-61 0.2086320580252945 1102 62-63 0.30529520817184874 1102 64-65 0.28909298388744276 1102 66-67 0.29542118986127264 1102 68-69 0.14034632624128562 1102 70-71 0.14371403206039446 1102 72-73 0.07607099621560565 1102 74-75 0.22927234301486266 1102 76-77 0.06249802151431538 1102 78-79 0.019927071122033624 1102 80-81 0.17648121099111336 1102 82-83 0.2837302698143489 1102 84-85 0.14659734993448836 1102 86-87 -0.02039813492367415 1102 88-89 0.014972313466227405 1102 90-91 0.06733153251357393 1102 92-93 0.05159986718096832 1102 94-95 0.05396562583114317 1102 96-97 0.1187127540449211 1102 98-99 0.3398872235522141 1102 100-101 0.5008978409480598 1103 1 -0.011960533699905795 1103 2 0.005549084486752065 1103 3 0.05452813743823981 1103 4 0.029671994096787557 1103 5 0.035288089567202974 1103 6 0.06224929585610539 1103 7 0.11020785227857033 1103 8 0.09156429724121296 1103 9 0.04022211404768683 1103 10-11 0.20272231753815362 1103 12-13 0.15968661072486157 1103 14-15 0.10735893322033263 1103 16-17 0.13890893681561067 1103 18-19 0.07634262612564413 1103 20-21 0.13882906520803573 1103 22-23 0.20918818576199527 1103 24-25 0.19381308429750987 1103 26-27 0.20466135988137069 1103 28-29 0.11418916091307807 1103 30-31 0.1403289612402716 1103 32-33 0.10564589792175383 1103 34-35 0.10918467707097079 1103 36-37 0.22541952680394672 1103 38-39 0.18265254996914848 1103 40-41 0.14110566571596195 1103 42-43 0.19315271900872943 1103 44-45 0.3006988958737722 1103 46-47 0.2944558396876218 1103 48-49 0.08637601702442765 1103 50-51 0.05917300383222113 1103 52-53 0.04086619419559412 1103 54-55 0.07913860281480822 1103 56-57 0.1842422814746314 1103 58-59 0.20812837400305995 1103 60-61 0.19535657211080348 1103 62-63 0.16494453207536708 1103 64-65 0.1469116509514805 1103 66-67 0.058214295549348094 1103 68-69 -0.07510187662968804 1103 70-71 -0.10168663964212499 1103 72-73 -0.054057517518380394 1103 74-75 0.0753376487006463 1103 76-77 0.1275844869758913 1103 78-79 0.0804700879757334 1103 80-81 0.09391443237402086 1103 82-83 0.2945265124067049 1103 84-85 0.25481004045079914 1103 86-87 0.25821083333666195 1103 88-89 0.182406012453324 1103 90-91 0.3463861054781212 1103 92-93 0.39757299525913226 1103 94-95 0.3676828000955332 1103 96-97 0.4585821006441897 1103 98-99 0.4184192020179971 1103 100-101 0.6489728095217053 1104 1 0.03960978929721648 1104 2 0.05742904335209431 1104 3 0.12201186748080062 1104 4 0.0383793300273112 1104 5 0.15945582588505403 1104 6 0.036446306837859765 1104 7 -0.031391445129493434 1104 8 0.028087067675301114 1104 9 0.03631694894608728 1104 10-11 0.10002172979297796 1104 12-13 0.12211839700480454 1104 14-15 0.09004184357230116 1104 16-17 0.10372491928083605 1104 18-19 0.23822494948307593 1104 20-21 0.22006719583308865 1104 22-23 0.19081551929049567 1104 24-25 0.18203097036652593 1104 26-27 0.0839193222146335 1104 28-29 0.13013071667381126 1104 30-31 0.07812593018660152 1104 32-33 0.11676370217183774 1104 34-35 0.16668781096127816 1104 36-37 0.026062818777432994 1104 38-39 0.10619782436283032 1104 40-41 0.23524810572166643 1104 42-43 0.23406218523402345 1104 44-45 0.24224339711607001 1104 46-47 0.1545060569957002 1104 48-49 0.1406523698516864 1104 50-51 0.31945103807606756 1104 52-53 0.28362634462233416 1104 54-55 0.10825751049452492 1104 56-57 0.13216809647376948 1104 58-59 0.21193032714858617 1104 60-61 0.21659008634654242 1104 62-63 0.2622762215237273 1104 64-65 0.012074613247214927 1104 66-67 0.016127274734309083 1104 68-69 0.27674955820415903 1104 70-71 0.18347110575837888 1104 72-73 0.06622006634602684 1104 74-75 0.22461685393106023 1104 76-77 0.08948332424628802 1104 78-79 0.07773497113979744 1104 80-81 0.198547898569295 1104 82-83 0.09180790948374806 1104 84-85 0.1723633916491778 1104 86-87 0.21816084121830315 1104 88-89 0.41285766797309975 1104 90-91 0.5169138375924298 1104 92-93 0.4138054210778819 1104 94-95 0.33362848433450765 1104 96-97 0.3296628611849428 1104 98-99 0.2504961643587684 1104 100-101 0.4351026238903124 1105 1 0.08501081286316747 1105 2 0.11641688057501653 1105 3 0.03387660304147033 1105 4 0.050792287944354086 1105 5 -0.03635237605801933 1105 6 0.004123123957477048 1105 7 0.07672248142248606 1105 8 -0.0036791002613085766 1105 9 0.0382522246254311 1105 10-11 0.01620004527826069 1105 12-13 0.20745952220218555 1105 14-15 0.14434762800706125 1105 16-17 0.12364126279725696 1105 18-19 0.14658987267937107 1105 20-21 0.11278267314367696 1105 22-23 0.06102514545143123 1105 24-25 0.15083224157524455 1105 26-27 0.12830511919469245 1105 28-29 0.052500554654521636 1105 30-31 0.17617325062293787 1105 32-33 0.18240353966307055 1105 34-35 0.04567189547164219 1105 36-37 0.07399579025501168 1105 38-39 0.04673636580854179 1105 40-41 0.14082523484217546 1105 42-43 0.19942392315690682 1105 44-45 0.07737694473473056 1105 46-47 -0.15692308290795864 1105 48-49 -0.040513454720787934 1105 50-51 -0.047509636396966926 1105 52-53 0.05292403443797156 1105 54-55 0.026039566727199315 1105 56-57 0.04854741775853455 1105 58-59 0.11783581861796932 1105 60-61 0.05624773743854661 1105 62-63 0.07148681940154233 1105 64-65 0.0855144728558841 1105 66-67 -0.03757209310584386 1105 68-69 -0.04822175258026817 1105 70-71 0.025941025138500606 1105 72-73 -0.09543550534438339 1105 74-75 -0.14689557081074156 1105 76-77 -0.10873694729056638 1105 78-79 -0.18862807932602976 1105 80-81 0.10248888127237166 1105 82-83 0.10167736222223311 1105 84-85 0.011209088085550434 1105 86-87 0.08412305847316759 1105 88-89 0.13140195995226378 1105 90-91 0.1509257864467628 1105 92-93 0.17831522979831504 1105 94-95 -0.09965662573599587 1105 96-97 -0.13069458015399604 1105 98-99 0.05853222665742308 1105 100-101 0.4255017076483192 1106 1 -0.09557771615326516 1106 2 -0.08039871966364842 1106 3 -0.1276557644030447 1106 4 -0.02642666843854613 1106 5 -0.09497886355765672 1106 6 -0.015576644804582429 1106 7 -0.00938529431712709 1106 8 0.02054938594069 1106 9 -0.05040042870061967 1106 10-11 0.01571657937748938 1106 12-13 0.0036872279055160107 1106 14-15 0.0688915295355983 1106 16-17 -0.1397227124962228 1106 18-19 -0.07464868656182233 1106 20-21 -0.10093054922499078 1106 22-23 -0.06694730068612387 1106 24-25 -0.028096424063221548 1106 26-27 -0.12295113163914806 1106 28-29 -0.11018322214197696 1106 30-31 -0.026016969415977087 1106 32-33 0.04477285720042801 1106 34-35 0.004443227182264309 1106 36-37 -0.049065946058988175 1106 38-39 0.06715992104946622 1106 40-41 -0.05766609080177432 1106 42-43 -0.053077170888407466 1106 44-45 -0.15411826210827684 1106 46-47 -0.13448179087847478 1106 48-49 -0.21638618129028941 1106 50-51 -0.03174126023832713 1106 52-53 -0.014637554772015449 1106 54-55 -0.011162754082953086 1106 56-57 0.01124883834432211 1106 58-59 0.06381583767848298 1106 60-61 0.07303019690454704 1106 62-63 -0.04214026998597831 1106 64-65 -0.03078352118433969 1106 66-67 0.07211795418906775 1106 68-69 0.07761344834381134 1106 70-71 -0.03970451808812214 1106 72-73 -0.19559768914138687 1106 74-75 -0.20463834783018342 1106 76-77 -0.21741747432706404 1106 78-79 -0.2817807680700284 1106 80-81 -0.2514051160584643 1106 82-83 -0.16612754614088487 1106 84-85 -0.04225898121890026 1106 86-87 -0.006349816808274511 1106 88-89 0.029807196072667352 1106 90-91 0.029229922785450668 1106 92-93 0.08546109482854192 1106 94-95 -0.08636468142953291 1106 96-97 0.1181990977634122 1106 98-99 -0.05802064625992287 1106 100-101 -0.041954409944498394 1107 1 -0.06732317462404325 1107 2 -0.027531916508685583 1107 3 0.00824020077597254 1107 4 0.009942614741738964 1107 5 -0.024878125679222762 1107 6 -0.015609120237606078 1107 7 0.03303814141901995 1107 8 0.026982266081638784 1107 9 0.053596885443852216 1107 10-11 -0.01583742040661207 1107 12-13 0.05567765176937911 1107 14-15 0.19008458546469598 1107 16-17 0.19981594424668003 1107 18-19 0.16302688660670128 1107 20-21 0.10827138759153598 1107 22-23 0.030411303108110133 1107 24-25 0.09731043365047753 1107 26-27 0.17113472608735947 1107 28-29 0.22231009523722634 1107 30-31 0.1679697696297353 1107 32-33 0.23153426979943248 1107 34-35 0.23707550282276202 1107 36-37 0.10530991271038204 1107 38-39 0.22474169967862423 1107 40-41 0.2200260768556248 1107 42-43 0.054789522490452214 1107 44-45 -0.039483871448034336 1107 46-47 0.07118512644364472 1107 48-49 0.05660253738463439 1107 50-51 0.2779179398231477 1107 52-53 0.29852876335549183 1107 54-55 0.36231799027226685 1107 56-57 0.19328804854178827 1107 58-59 0.2540864266456069 1107 60-61 0.3464376657052597 1107 62-63 0.22721737509687046 1107 64-65 0.24786484214531157 1107 66-67 0.15199974361286905 1107 68-69 0.20061460831139755 1107 70-71 0.3269426633187251 1107 72-73 0.37776934545821206 1107 74-75 0.3453216753691706 1107 76-77 0.21032171409814282 1107 78-79 0.16329412668212484 1107 80-81 0.2531210902437806 1107 82-83 0.4374791480064033 1107 84-85 0.5421332341911054 1107 86-87 0.4439181741242173 1107 88-89 0.556387423199439 1107 90-91 0.3605653308872938 1107 92-93 0.6267440025050064 1107 94-95 0.45240801249454776 1107 96-97 0.43013683824170457 1107 98-99 0.5350274155513439 1107 100-101 0.7280639501101724 1108 1 -0.24502986336014487 1108 2 -0.15239398254722403 1108 3 -0.10990023721740982 1108 4 -0.01233902518696084 1108 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-0.46325881537931934 2316 64-65 -0.4694554192925935 2316 66-67 -0.6400170631098163 2316 68-69 -0.6251323571414531 2316 70-71 -0.49261849447772477 2316 72-73 -0.648141018687447 2316 74-75 -0.6773207149202776 2316 76-77 -0.506181553875706 2316 78-79 -0.7248944820947187 2316 80-81 -0.6382187790372598 2316 82-83 -0.7891781815920993 2316 84-85 -0.5164568685166486 2316 86-87 -0.7361705280233082 2316 88-89 -0.6876601958481778 2316 90-91 -0.780424398337729 2316 92-93 -0.6875650544511132 2316 94-95 -0.5544780752738951 2316 96-97 -0.507654945138313 2316 98-99 -0.618800782394505 2316 100-101 -0.5423608864911351 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 11.0 5 29.0 6 51.0 7 138.0 8 302.0 9 542.0 10 882.0 11 1436.0 12 2097.0 13 3146.0 14 4114.0 15 5295.0 16 6677.0 17 9353.0 18 8866.0 19 5714.0 20 4975.0 21 5358.0 22 5867.0 23 6602.0 24 8008.0 25 9847.0 26 11983.0 27 15029.0 28 18773.0 29 23165.0 30 29349.0 31 36492.0 32 45850.0 33 59119.0 34 82085.0 35 120462.0 36 201169.0 37 373287.0 38 438872.0 39 89208.0 40 123.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.32700648962629 18.590098319862868 13.205266793702522 23.87762839680832 2 15.972288522503336 19.502503090613104 39.973500287641215 24.55170809924234 3 20.073059696012923 23.42499449298774 28.505152115916488 27.99679369508285 4 12.860403358053698 15.48645274983479 36.366595687397506 35.286548204714 5 13.804244070783463 37.46090021293781 34.26591526543799 14.468940450840737 6 33.946141420074895 35.311819272095356 16.709254228161637 14.03278507966811 7 30.199292654869424 31.269060381330004 19.877805516802507 18.653841446998065 8 26.908118559855104 35.967949188633526 18.877303766796388 18.246628484714982 9 26.65895684949947 15.882039797341948 19.634517952859976 37.8244854002986 10-11 25.856765013178645 26.298460018142627 26.6790880957735 21.165686872905223 12-13 26.060933885544056 23.12964214505536 27.707928784519414 23.101495184881166 14-15 22.665455123968965 24.950589862202317 25.552842842107836 26.83111217172088 16-17 22.850317618778533 28.199187343833227 26.12477554970245 22.825719487685788 18-19 22.68099713635362 26.86855985510439 28.12697334606065 22.323469662481337 20-21 23.21510684990349 26.231912125136564 28.292668599629746 22.260312425330202 22-23 22.914042549025424 26.053835956456652 28.230024301228767 22.802097193289153 24-25 22.66223566507551 26.208372928628116 28.38418154460506 22.745209861691315 26-27 22.63600575557529 26.53949859919724 28.236034882237416 22.58846076299005 28-29 22.48779249069904 26.57045476796554 28.380317456432348 22.561435284903077 30-31 22.66130603945574 26.28205461610642 28.338059729119237 22.718579615318603 32-33 22.563148785486725 26.337711575699146 28.402596778777863 22.696542860036267 34-35 22.84992244172421 26.467228186543718 28.158518713419877 22.5243306583122 36-37 22.85334301751934 26.453889925193337 28.088157503046453 22.604609554240877 38-39 22.71727943471062 26.55720140637027 28.251864125932062 22.473655032987043 40-41 22.8287526892774 26.43330224908032 28.115523280147443 22.622421781494833 42-43 22.74626151468164 26.36331260578454 27.982099083476008 22.90832679605781 44-45 22.753300157110274 26.56607733741688 27.981783163347256 22.69883934212559 46-47 22.72222116826871 26.88473916792478 27.619412762941852 22.773626900864656 48-49 23.07045016072251 26.741226815338177 27.537908633728627 22.650414390210692 50-51 22.86341516399023 26.93860941427363 27.385809594181747 22.8121658275544 52-53 22.89627892631775 27.00060949042684 27.310066627828988 22.793044955426424 54-55 22.94177018609787 26.711424549547825 27.497308438698642 22.849496825655667 56-57 22.804684775092856 27.097838125600436 27.395684817926487 22.70179228138022 58-59 22.960436040330364 26.895415488120715 27.405893520126174 22.738254951422746 60-61 23.025956499404774 26.910023444955144 27.568886956644118 22.495133098995964 62-63 22.892068678561813 26.96533029835769 27.489383704138824 22.653217318941675 64-65 22.789032603095464 26.79683551352231 27.522667724024668 22.891464159357557 66-67 22.801386543309594 26.96832856058937 27.36238588246812 22.867899013632915 68-69 22.612924345889322 26.831601683921974 27.56118902513645 22.994284945052254 70-71 22.66694854479904 26.72302597602853 27.578450641140172 23.031574838032252 72-73 22.85281408989264 26.944600564356914 27.367302238734048 22.8352831070164 74-75 22.740778813911934 26.983503438823213 27.1808074503757 23.09491029688915 76-77 22.815239504686364 27.062256290505854 27.225023043296876 22.897481161510903 78-79 22.740178788271358 26.93327183639118 27.33070501282769 22.995844362509775 80-81 22.543672698146377 27.102925546073177 27.295795831627668 23.05760592415278 82-83 22.899258389015344 27.19943950852975 27.06773013192354 22.833571970531366 84-85 22.831788553587465 26.9680759116852 27.06157345722166 23.13856207750567 86-87 22.712189414000818 27.024577321229735 27.27244880299088 22.990784461778567 88-89 22.871524554928836 26.98210852079543 27.15152608137952 22.99484084289622 90-91 22.838419330276814 27.161749464689784 27.14594411781766 22.853887087215746 92-93 22.798301404112458 27.082840456383344 27.164462413549 22.954395725955198 94-95 22.669176302421715 27.089992286908643 27.217513765619316 23.02331764505033 96-97 22.84029881843907 27.03819184065464 27.0785776722035 23.042931668702785 98-99 22.707050557371733 26.924810270264484 27.034005259349573 23.334133913014213 100-101 22.616170618439526 27.13333474069254 26.91959971032876 23.330894930539177 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 152.0 1 117.0 2 168.5 3 279.5 4 214.5 5 173.0 6 387.5 7 804.5 8 1201.0 9 1335.5 10 1120.5 11 897.5 12 938.5 13 1084.5 14 1169.0 15 1221.0 16 1330.0 17 1440.5 18 1552.5 19 1719.5 20 1965.0 21 2299.5 22 2860.5 23 3625.5 24 4510.0 25 5589.0 26 7179.0 27 9154.5 28 10993.0 29 13318.5 30 16425.5 31 19568.5 32 23059.5 33 26464.0 34 30152.0 35 33865.5 36 38104.5 37 42033.0 38 44428.0 39 47102.5 40 48663.0 41 50992.0 42 53248.5 43 54782.0 44 57902.5 45 60385.5 46 61585.0 47 62069.5 48 62111.5 49 61118.0 50 59075.5 51 57277.5 52 56754.0 53 56477.0 54 55719.5 55 52455.0 56 47313.0 57 43357.0 58 40473.5 59 36407.0 60 31429.5 61 26456.5 62 21540.5 63 17621.0 64 13430.5 65 9805.5 66 7899.5 67 6573.0 68 5083.0 69 3784.0 70 2869.5 71 2183.0 72 1694.0 73 1595.0 74 1385.5 75 983.0 76 638.0 77 393.5 78 274.0 79 190.0 80 136.0 81 100.0 82 61.0 83 32.0 84 10.5 85 5.0 86 3.0 87 0.5 88 0.5 89 2.0 90 2.5 91 2.5 92 1.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26274567393592285 2 0.01835670754093546 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.589176885233864E-4 12-13 3.0594512568225764E-5 14-15 0.0 16-17 5.812957387962895E-4 18-19 0.0 20-21 9.178353770467729E-5 22-23 3.0594512568225764E-5 24-25 0.0022639939300487066 26-27 0.0023557774677533836 28-29 0.002447561005458061 30-31 0.0011319969650243533 32-33 0.0014991311158430623 34-35 0.0015297256284112883 36-37 3.0594512568225764E-5 38-39 1.2237805027290305E-4 40-41 7.342683016374183E-4 42-43 0.003793719558459995 44-45 0.004742149448074993 46-47 0.013522774555155787 48-49 0.01545022884695401 50-51 0.006883765327850797 52-53 0.007832195217465796 54-55 3.365396382504834E-4 56-57 0.0030594512568225766 58-59 0.0016826981912524172 60-61 0.0016215091661159653 62-63 0.0 64-65 0.0011014024524561275 66-67 0.0 68-69 0.0 70-71 2.447561005458061E-4 72-73 0.0018662652666617716 74-75 0.0 76-77 0.19038965171206892 78-79 0.1027057786915339 80-81 0.001284969527865482 82-83 0.0 84-85 7.648628142056441E-4 86-87 0.12130724233301515 88-89 0.5871698852093888 90-91 0.31111559830628777 92-93 0.2947169395697188 94-95 0.0027841006437085442 96-97 0.0026617225934356414 98-99 0.0029064786939814475 100-101 8.260518393420957E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1634280.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.976959102149515 #Duplication Level Percentage of deduplicated Percentage of total 1 74.53726204578057 40.97832007077079 2 14.287183920646848 15.709318521805798 3 4.8406159061958745 7.983670281124349 4 2.1663284252919195 4.763925969563912 5 1.140546011981996 3.135187572742695 6 0.6594772196593195 2.1753631280405807 7 0.4399423434638465 1.6930684556740987 8 0.3088513832115977 1.3583767890773049 9 0.22289659232515333 1.1028759156241603 >10 1.2553230579934913 12.894537660256203 >50 0.10092290438346667 3.8085730379810627 >100 0.03953659247378883 3.500245369794855 >500 7.795176144461156E-4 0.3083147690462334 >1k 3.3407897761976386E-4 0.5882224584980561 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4104 0.25111975915999707 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3219 0.19696747191423747 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2285 0.13981692243679172 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.0594512568225764E-5 0.0 16-17 0.0 0.0 0.0 1.2237805027290305E-4 0.0 18-19 0.0 0.0 0.0 1.8356707540935458E-4 0.0 20-21 0.0 0.0 0.0 2.447561005458061E-4 0.0 22-23 0.0 0.0 0.0 5.812957387962895E-4 0.0 24-25 0.0 0.0 0.0 7.648628142056441E-4 0.0 26-27 0.0 0.0 0.0 9.484298896149986E-4 0.0 28-29 0.0 0.0 0.0 0.0011931859901608047 0.0 30-31 0.0 0.0 0.0 0.002202804904912255 0.0 32-33 0.0 0.0 0.0 0.005048094573757251 0.0 34-35 0.0 0.0 0.0 0.007587439116919989 0.0 36-37 0.0 0.0 0.0 0.012972073328927722 0.0 38-39 0.0 0.0 0.0 0.02404728687862545 0.0 40-41 0.0 0.0 0.0 0.035642607141983015 0.0 42-43 0.0 0.0 0.0 0.04772743960643219 0.0 44-45 0.0 0.0 0.0 0.061923293438088944 0.0 46-47 0.0 0.0 0.0 0.07688401008395135 0.0 48-49 0.0 0.0 0.0 0.09579141885111486 0.0 50-51 0.0 0.0 0.0 0.11818660205105612 0.0 52-53 0.0 0.0 0.0 0.1404900017132927 0.0 54-55 0.0 0.0 0.0 0.16392539834055364 0.0 56-57 0.0 0.0 0.0 0.191888782827912 0.0 58-59 0.0 0.0 0.0 0.22190199965734148 0.0 60-61 0.0 0.0 0.0 0.2599615732922143 0.0 62-63 0.0 0.0 0.0 0.2974092566757226 0.0 64-65 0.0 0.0 0.0 0.3388954157182368 0.0 66-67 0.0 0.0 0.0 0.3842670778569156 0.0 68-69 0.0 0.0 0.0 0.43832758156497054 0.0 70-71 0.0 0.0 0.0 0.4982010426609883 0.0 72-73 0.0 0.0 0.0 0.5612869275766699 0.0 74-75 0.0 0.0 0.0 0.6263920503218543 0.0 76-77 0.0 0.0 0.0 0.7013486061140074 0.0 78-79 0.0 0.0 0.0 0.7892772352350882 0.0 80-81 0.0 0.0 0.0 0.8915240962380987 0.0 82-83 0.0 0.0 0.0 0.9941074968793597 0.0 84-85 0.0 0.0 0.0 1.0969662481337346 0.0 86-87 0.0 0.0 0.0 1.2101353501236018 0.0 88-89 0.0 0.0 0.0 1.340773918789926 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2675 0.0 48.370586 1 GTATCAA 4600 0.0 40.124546 1 TCAACGC 5605 0.0 31.996923 4 ATCAACG 5690 0.0 31.602324 3 CAACGCA 5775 0.0 31.055023 5 TATCAAC 5995 0.0 30.079195 2 AACGCAG 5985 0.0 30.044647 6 TACATGG 6310 0.0 26.32149 2 GTACATG 6440 0.0 26.148912 1 ACGCAGA 7005 0.0 25.669838 7 CGCAGAG 7215 0.0 24.92269 8 GTATAGG 555 0.0 24.856573 1 ACATGGG 6460 0.0 24.677366 3 GCAGAGT 8135 0.0 21.929176 9 CGTTCGG 55 0.0028978428 21.565989 34-35 GTGGTAT 1565 0.0 20.973503 1 CATGGGG 3485 0.0 20.965729 4 GAGTACA 5115 0.0 19.995352 1 AGTACAT 4700 0.0 19.890244 2 TTACGCG 60 0.0047701807 19.788197 80-81 >>END_MODULE