##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936370_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 967900 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.051365843578886 33.0 31.0 34.0 30.0 34.0 2 32.145402417605126 34.0 31.0 34.0 30.0 34.0 3 32.18751317284843 34.0 31.0 34.0 30.0 34.0 4 35.724676102903196 37.0 35.0 37.0 35.0 37.0 5 35.76903502427937 37.0 35.0 37.0 35.0 37.0 6 35.88188655852878 37.0 36.0 37.0 35.0 37.0 7 35.83813307159831 37.0 35.0 37.0 35.0 37.0 8 35.84985225746461 37.0 36.0 37.0 35.0 37.0 9 37.5627110238661 39.0 38.0 39.0 35.0 39.0 10-11 37.51636790990805 39.0 37.5 39.0 35.0 39.0 12-13 37.469925612150014 39.0 37.0 39.0 35.0 39.0 14-15 38.724486517202195 40.0 38.0 41.0 35.0 41.0 16-17 38.713757619588804 40.0 38.0 41.0 35.0 41.0 18-19 38.7934146089472 40.0 38.5 41.0 35.0 41.0 20-21 38.75381444364088 40.0 38.5 41.0 35.0 41.0 22-23 38.5953734889968 40.0 38.0 41.0 34.5 41.0 24-25 38.72448393429073 40.0 38.0 41.0 35.0 41.0 26-27 38.64812583944622 40.0 38.0 41.0 35.0 41.0 28-29 38.529064986052276 40.0 38.0 41.0 35.0 41.0 30-31 38.33127079243724 40.0 38.0 41.0 34.0 41.0 32-33 38.082543651203636 40.0 38.0 41.0 34.0 41.0 34-35 37.80892757516273 40.0 37.5 41.0 33.5 41.0 36-37 37.583952371112716 40.0 37.0 41.0 33.0 41.0 38-39 37.40323690463891 40.0 37.0 41.0 33.0 41.0 40-41 37.2528458518442 40.0 37.0 41.0 33.0 41.0 42-43 37.03096032648001 40.0 37.0 41.0 32.5 41.0 44-45 36.77224041739849 40.0 37.0 41.0 31.5 41.0 46-47 36.379880152908356 40.0 37.0 41.0 30.5 41.0 48-49 36.107406756896374 40.0 37.0 41.0 30.0 41.0 50-51 35.750912800909184 39.5 36.5 40.5 28.5 41.0 52-53 35.80860626097737 39.5 37.0 40.5 28.0 41.0 54-55 35.94461669593966 40.0 37.0 41.0 28.0 41.0 56-57 35.8584760822399 40.0 36.5 41.0 28.0 41.0 58-59 35.72152443434239 40.0 36.0 41.0 27.5 41.0 60-61 35.543477115404485 40.0 36.0 41.0 27.0 41.0 62-63 35.27647329269553 39.0 35.5 41.0 26.0 41.0 64-65 35.035160657092675 39.0 35.0 41.0 26.0 41.0 66-67 34.73523762785412 39.0 35.0 40.5 25.5 41.0 68-69 34.37054034507697 38.0 35.0 40.0 24.0 41.0 70-71 33.998304060336814 37.0 35.0 40.0 22.5 41.0 72-73 33.59214278334539 37.0 34.0 39.0 22.0 41.0 74-75 33.142658332472365 36.0 34.0 39.0 20.5 40.5 76-77 32.73403657402624 36.0 34.0 38.5 19.0 40.0 78-79 32.310754210145674 35.5 34.0 37.0 15.5 39.5 80-81 31.8709303647071 35.0 33.0 37.0 9.0 39.0 82-83 31.472371629300547 35.0 33.0 36.5 4.5 39.0 84-85 31.080822399008163 35.0 33.0 36.0 2.0 37.0 86-87 30.763285979956606 35.0 32.5 36.0 2.0 37.0 88-89 30.41240210765575 35.0 32.0 35.0 2.0 36.5 90-91 30.07942246099804 35.0 32.0 35.0 2.0 36.0 92-93 29.79424424010745 35.0 31.5 35.0 2.0 36.0 94-95 29.455446327099907 34.0 31.0 35.0 2.0 36.0 96-97 29.121289905982024 34.0 31.0 35.0 2.0 35.0 98-99 28.79418070048559 34.0 31.0 35.0 2.0 35.0 100-101 25.942179977270378 31.5 24.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2256417985564383 1101 2 0.10525206992895164 1101 3 0.1836925424453426 1101 4 -0.04428736585666826 1101 5 0.1396252201306254 1101 6 0.08450396491038248 1101 7 0.27535668988915774 1101 8 0.17705687831303862 1101 9 0.346505131207536 1101 10-11 0.4882081525347246 1101 12-13 0.2951478990608578 1101 14-15 0.49364980400721237 1101 16-17 0.36283830184385835 1101 18-19 0.3269005279144963 1101 20-21 0.5074011941203267 1101 22-23 0.2811298729616567 1101 24-25 0.287011504129417 1101 26-27 0.15313478002229175 1101 28-29 0.22984847228737237 1101 30-31 0.4649270612366294 1101 32-33 0.3886630355280616 1101 34-35 0.2153031042679956 1101 36-37 0.20842971941883803 1101 38-39 0.3413615855962533 1101 40-41 0.3049016676192977 1101 42-43 0.0841240220923325 1101 44-45 0.16221180101263144 1101 46-47 -0.052814788081150255 1101 48-49 -0.057792580155705764 1101 50-51 -0.05711744540563757 1101 52-53 0.0861282232503342 1101 54-55 0.029214514847190287 1101 56-57 -0.005974920142364226 1101 58-59 0.09389779535258214 1101 60-61 -0.03266794121961425 1101 62-63 0.025888038447405393 1101 64-65 -0.0734661150304845 1101 66-67 -0.05546402628352354 1101 68-69 -0.12363463146523657 1101 70-71 0.027013658632803583 1101 72-73 0.06842326428738232 1101 74-75 -0.0885872108404655 1101 76-77 -0.045858428888379876 1101 78-79 0.017057756695471937 1101 80-81 -0.15366548553908288 1101 82-83 -0.15438685306378375 1101 84-85 -0.15337314020994341 1101 86-87 -0.14797082463994826 1101 88-89 -0.3484535086061733 1101 90-91 -0.2858312272388801 1101 92-93 -0.430241075667535 1101 94-95 -0.12671282793966654 1101 96-97 -0.10462398912923376 1101 98-99 -0.19984109523067417 1101 100-101 0.035201893220456526 1102 1 0.2469216898178388 1102 2 0.1853424746957515 1102 3 0.23346732077980903 1102 4 0.2704413408177899 1102 5 0.25231665854633434 1102 6 0.2130051739951071 1102 7 0.18791536605991155 1102 8 0.27063213395938135 1102 9 0.3734558669316428 1102 10-11 0.41492655433017234 1102 12-13 0.3181062844190805 1102 14-15 0.49158250619304766 1102 16-17 0.42908745232354306 1102 18-19 0.5016733306203207 1102 20-21 0.38607388777144536 1102 22-23 0.44193656164999595 1102 24-25 0.5213060466528532 1102 26-27 0.3505395942341707 1102 28-29 0.4289855176952244 1102 30-31 0.4707772927803049 1102 32-33 0.28884953487215626 1102 34-35 0.11878653269491934 1102 36-37 0.008888855515948535 1102 38-39 0.05174153849727503 1102 40-41 0.1219307962880336 1102 42-43 0.014324271811794631 1102 44-45 -0.1341214322221802 1102 46-47 -0.0278997401128791 1102 48-49 0.06435816821242213 1102 50-51 -0.09997436247664382 1102 52-53 -0.07092161000661434 1102 54-55 -0.024162229632899823 1102 56-57 0.03462108750726145 1102 58-59 0.056406685681487545 1102 60-61 -0.025005663082708907 1102 62-63 0.31793415752248677 1102 64-65 0.2923065138200087 1102 66-67 0.18996832028916089 1102 68-69 0.0668049063957099 1102 70-71 0.10410505911643497 1102 72-73 0.1564470034411798 1102 74-75 0.20675662756891455 1102 76-77 0.10073953858486817 1102 78-79 0.10650552897661925 1102 80-81 0.05898406954934998 1102 82-83 0.1144906737652569 1102 84-85 0.21805926958285227 1102 86-87 -0.004808870506138874 1102 88-89 -0.09860444663465628 1102 90-91 -0.045844503463374764 1102 92-93 0.08696032781905316 1102 94-95 0.07628143483474759 1102 96-97 0.1695616567432836 1102 98-99 -0.013709218067361917 1102 100-101 0.441256009640302 1103 1 0.27207616848275507 1103 2 0.30314766128651627 1103 3 0.2999782924141172 1103 4 0.159599857822613 1103 5 0.12641958755912697 1103 6 0.0788276816582254 1103 7 0.08156531983794935 1103 8 0.24037803480111108 1103 9 0.34536081997985946 1103 10-11 0.3321907615018773 1103 12-13 0.29842393877611073 1103 14-15 0.31705354448429546 1103 16-17 0.5336950521873121 1103 18-19 0.2618254373687279 1103 20-21 0.36982625968795446 1103 22-23 0.5411039581371284 1103 24-25 0.5726139457430008 1103 26-27 0.47157879775677713 1103 28-29 0.4010166688653669 1103 30-31 0.27988818899984125 1103 32-33 0.44086657617999236 1103 34-35 0.3162272956005765 1103 36-37 0.17372336872569605 1103 38-39 0.37506568825643427 1103 40-41 0.39907707663640224 1103 42-43 0.29913822841179893 1103 44-45 0.3993043450366116 1103 46-47 0.288252403126549 1103 48-49 0.37232871995669825 1103 50-51 0.4484659503730626 1103 52-53 0.5586686566211299 1103 54-55 0.6624619858100971 1103 56-57 0.48456148550268807 1103 58-59 0.3752789306092339 1103 60-61 0.26456885158668086 1103 62-63 0.2694725582425832 1103 64-65 0.3998470796741458 1103 66-67 0.4648658413323119 1103 68-69 0.4230986956235583 1103 70-71 0.3057955057665609 1103 72-73 0.35766002587099877 1103 74-75 0.2436642566255003 1103 76-77 0.1751614599588791 1103 78-79 0.11856581214695439 1103 80-81 0.20016631788292827 1103 82-83 0.2132703531307989 1103 84-85 0.21066223508667292 1103 86-87 0.24623112365529565 1103 88-89 0.30244102118026106 1103 90-91 0.38552196371903236 1103 92-93 0.4668349203359625 1103 94-95 0.5688996049315698 1103 96-97 0.481819903714527 1103 98-99 0.6033038092279241 1103 100-101 0.9815527424773585 1104 1 0.19124243475589253 1104 2 0.014115012054126908 1104 3 0.16799714522945663 1104 4 -0.0402680123126089 1104 5 0.16744705455526088 1104 6 0.06528866044880743 1104 7 0.2019336246861485 1104 8 0.18124420040387434 1104 9 0.07284869377582481 1104 10-11 0.19723311741739735 1104 12-13 0.29633259757218156 1104 14-15 0.24669367649976692 1104 16-17 0.2699100749525414 1104 18-19 0.17209224245716115 1104 20-21 0.24836545249459618 1104 22-23 0.4086752281854089 1104 24-25 0.2867937395975346 1104 26-27 0.21432809992805346 1104 28-29 0.3272992151394192 1104 30-31 0.2791739155425006 1104 32-33 0.4140352047749545 1104 34-35 0.45142303083749624 1104 36-37 0.33429761884581666 1104 38-39 0.313288541575119 1104 40-41 0.398852570264431 1104 42-43 0.44423297868063827 1104 44-45 0.5603998217337107 1104 46-47 0.6897184154357916 1104 48-49 0.5687925301015184 1104 50-51 0.43846111705116186 1104 52-53 0.6641458971089236 1104 54-55 0.7453305766536715 1104 56-57 0.7310294425805068 1104 58-59 0.7899758041077547 1104 60-61 0.6720693663783592 1104 62-63 0.8157430355116659 1104 64-65 0.8388535603294116 1104 66-67 0.7239397596977923 1104 68-69 0.6662240817173242 1104 70-71 0.721672110200636 1104 72-73 0.614636168136542 1104 74-75 0.6305545144789377 1104 76-77 0.7130665724122665 1104 78-79 0.8080692097720501 1104 80-81 0.746316677093116 1104 82-83 0.6108518332140846 1104 84-85 0.4368066455929984 1104 86-87 0.7004747230787274 1104 88-89 0.6113031599680987 1104 90-91 0.5788634193211131 1104 92-93 0.629476202052853 1104 94-95 0.36423916724424643 1104 96-97 0.474280350553709 1104 98-99 0.5931302386328241 1104 100-101 0.9277191174287367 1105 1 0.1916233688061837 1105 2 0.12126975548501662 1105 3 0.30142698185552774 1105 4 0.04364574421840217 1105 5 0.13787855258843962 1105 6 0.1626326813213197 1105 7 0.21055342710835134 1105 8 0.07326075833725554 1105 9 0.11285879855273606 1105 10-11 0.3008565353715582 1105 12-13 0.21975883871547097 1105 14-15 0.2916572605411645 1105 16-17 0.3038419425585843 1105 18-19 0.23145981173703234 1105 20-21 0.27252402264193876 1105 22-23 0.3219686235909478 1105 24-25 0.29795042913361414 1105 26-27 0.3924588765924355 1105 28-29 0.25067934321346286 1105 30-31 0.3662768252103348 1105 32-33 0.2987665660728638 1105 34-35 0.37393904009218915 1105 36-37 0.35594870731474515 1105 38-39 0.3734801313532543 1105 40-41 0.36690690616271837 1105 42-43 0.4400801292271055 1105 44-45 0.30567857857883496 1105 46-47 0.4956592490264171 1105 48-49 0.4512844001669265 1105 50-51 0.5521251546045818 1105 52-53 0.40736694177711996 1105 54-55 0.5198376680417525 1105 56-57 0.6175124322043928 1105 58-59 0.6364620550617701 1105 60-61 0.6012964979716955 1105 62-63 0.4790461213447941 1105 64-65 0.3964253931301158 1105 66-67 0.40144567085862803 1105 68-69 0.501031028350468 1105 70-71 0.5491016340598023 1105 72-73 0.6354525432215752 1105 74-75 0.562405412881823 1105 76-77 0.5248283456134857 1105 78-79 0.5197769839008757 1105 80-81 0.6287109745772135 1105 82-83 0.6352790621126729 1105 84-85 0.7796960771458323 1105 86-87 0.773410895907837 1105 88-89 0.7208638026639882 1105 90-91 0.6835561258374874 1105 92-93 0.5602711780005407 1105 94-95 0.3673949051134997 1105 96-97 0.5810488134914991 1105 98-99 0.5841528928476265 1105 100-101 0.6104545179641825 1106 1 0.12250780871563904 1106 2 0.11451418741915376 1106 3 0.17145657656053714 1106 4 0.13476381419013705 1106 5 0.13929769101894607 1106 6 0.019753198622687762 1106 7 0.0699336193762008 1106 8 0.015435754134578872 1106 9 0.033914825199694576 1106 10-11 0.0888849494159416 1106 12-13 0.09911447949752272 1106 14-15 0.05098623872240182 1106 16-17 0.057502185962320596 1106 18-19 -0.02349837645714814 1106 20-21 0.09826516084569192 1106 22-23 -0.08238613755985114 1106 24-25 0.0021147989853247395 1106 26-27 -0.1441631048062959 1106 28-29 -0.04695855364684576 1106 30-31 -0.016126249198649134 1106 32-33 0.043667636877309235 1106 34-35 -0.119193971882531 1106 36-37 -0.1707627619040295 1106 38-39 -0.1396381028163134 1106 40-41 -0.09393386494159728 1106 42-43 -0.17392399135665215 1106 44-45 -0.021026244537097227 1106 46-47 -0.10849691093354608 1106 48-49 -0.03351557168487318 1106 50-51 -0.039565653053294625 1106 52-53 -0.02383025591316823 1106 54-55 0.04134758441814057 1106 56-57 -0.004906827419745241 1106 58-59 -0.1490447493747027 1106 60-61 0.056151867045421966 1106 62-63 0.128769135235153 1106 64-65 -0.17396465491729884 1106 66-67 -0.1014883880727595 1106 68-69 -0.18721006098488147 1106 70-71 0.09913310531150188 1106 72-73 0.18145921278125599 1106 74-75 0.20522234402428552 1106 76-77 0.20309050700431186 1106 78-79 0.09910017606316757 1106 80-81 0.21483664847249528 1106 82-83 0.2803732608467868 1106 84-85 0.16423372340896236 1106 86-87 0.20508309447775375 1106 88-89 0.2530993561866204 1106 90-91 0.19324966230700014 1106 92-93 -0.18893617649867522 1106 94-95 -0.22459693190842245 1106 96-97 -0.19277062362268538 1106 98-99 -0.02983754458322352 1106 100-101 0.2298107060723531 1107 1 -0.13925075946821508 1107 2 -0.09599636765290853 1107 3 0.05923499156851619 1107 4 -0.06329535196518066 1107 5 -0.013598787593515738 1107 6 0.018293362172499883 1107 7 0.16145547213859146 1107 8 0.09954746638102563 1107 9 -0.0198050172128319 1107 10-11 -0.036960438931295414 1107 12-13 0.04134532883625042 1107 14-15 0.07545988880816168 1107 16-17 0.021178059491290924 1107 18-19 0.09777031767102784 1107 20-21 0.1949325360410299 1107 22-23 0.17300904096931902 1107 24-25 0.24622097668699183 1107 26-27 0.19273225620244006 1107 28-29 0.2521916129910835 1107 30-31 0.10832748187328178 1107 32-33 0.19949180946955636 1107 34-35 0.1916449127061739 1107 36-37 0.06150029206848018 1107 38-39 0.15778886859789765 1107 40-41 0.27049853005913604 1107 42-43 -0.13657249829956442 1107 44-45 0.08472633992152367 1107 46-47 0.03251232801306969 1107 48-49 -0.07240109617657708 1107 50-51 -0.02932473993361384 1107 52-53 0.04083568618265332 1107 54-55 0.08310465533384104 1107 56-57 0.10563300459210723 1107 58-59 0.19171677190321645 1107 60-61 0.31085522747663674 1107 62-63 0.3309177150362075 1107 64-65 0.03982585320471088 1107 66-67 0.09587160732849043 1107 68-69 0.15677058682422995 1107 70-71 0.19882576428594945 1107 72-73 0.12161059728640566 1107 74-75 0.12746733719968262 1107 76-77 0.16305128392606605 1107 78-79 0.11359760864933577 1107 80-81 0.2140031246385341 1107 82-83 0.34212755112162796 1107 84-85 0.29043070240309277 1107 86-87 0.1853425496593033 1107 88-89 0.2399842229498077 1107 90-91 0.3912620388325081 1107 92-93 -0.06503790517497166 1107 94-95 0.06029309240881631 1107 96-97 0.11945858139476684 1107 98-99 0.31022948490360136 1107 100-101 0.2185410895200519 1108 1 0.016157818421348225 1108 2 0.0045091994222872245 1108 3 0.13009305970332008 1108 4 0.13722341264410431 1108 5 0.11972041761037389 1108 6 0.09219955308498129 1108 7 0.030917901043217455 1108 8 0.1180963467479188 1108 9 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-1.5329014154362923 2316 88-89 -1.385431279638798 2316 90-91 -1.5871449511481792 2316 92-93 -1.628489601877984 2316 94-95 -1.3589128175057574 2316 96-97 -1.5687432012743017 2316 98-99 -1.3472034506380481 2316 100-101 -1.4644248574233636 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4313.0 3 681.0 4 597.0 5 499.0 6 563.0 7 624.0 8 827.0 9 988.0 10 1366.0 11 2223.0 12 3798.0 13 6385.0 14 6592.0 15 6493.0 16 8584.0 17 12487.0 18 10369.0 19 4572.0 20 3673.0 21 3490.0 22 3541.0 23 3937.0 24 4283.0 25 4839.0 26 5618.0 27 6646.0 28 8092.0 29 9991.0 30 12399.0 31 15591.0 32 20065.0 33 26531.0 34 37310.0 35 61392.0 36 90586.0 37 187493.0 38 313605.0 39 76829.0 40 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.03808496034797 23.674303505420628 14.023543129325958 21.26406840490544 2 14.25019890678763 24.220647041196955 40.951446078178115 20.5777079738373 3 15.325147136622139 31.92224077502976 32.447964885597145 20.304647202750957 4 9.58979265424879 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17.719926999303475 31.623077066165667 33.694926534265065 16.962069400265793 26-27 16.793703833433042 31.73573376233562 34.58691481018818 16.88364759404315 28-29 17.326570249492484 30.151301352840665 35.20997370581619 17.31215469185066 30-31 17.43460550435963 30.961712935885803 34.55042769663815 17.05325386311641 32-33 16.629820467129555 32.253762936425964 34.428730632495714 16.687685963948763 34-35 17.180719883429585 31.20087106651258 34.594176555692364 17.02423249436547 36-37 18.02787350459826 30.31551179373461 33.42951867360779 18.227096028059332 38-39 16.793827355941794 32.07148474097682 33.973522824301284 17.161165078780108 40-41 16.66015106842832 31.248964902471098 35.33442670782655 16.756457321274027 42-43 16.9976764081714 31.24961065820566 33.53499507871972 18.217717854903213 44-45 16.91218083244982 31.825323230319842 33.51502004645835 17.747475890771984 46-47 17.547300307494837 31.16856345598857 33.395434620740794 17.888701615775805 48-49 17.34078112496826 31.80997018840863 32.93939382924464 17.90985485737846 50-51 17.390358257199004 31.562768755219306 32.33127884899021 18.715594138591484 52-53 17.263836882463547 32.096395377034824 32.687533395611226 17.9522343448904 54-55 18.220994366134295 31.568650764188117 32.15607640790829 18.054278461769307 56-57 18.22157222497381 32.20471590655407 31.332151708942447 18.241560159529676 58-59 17.32770982007042 31.230174675708312 32.48435519089938 18.95776031332189 60-61 18.22366057615835 32.868498595216884 30.97224904426988 17.93559178435489 62-63 17.829083505254182 32.49381967466726 32.26384200793864 17.413254812139915 64-65 17.811798241401064 31.38291713504659 32.20453123499129 18.60075338856106 66-67 17.871506581952577 32.243833312570075 31.452130310203064 18.432529795274284 68-69 17.613398290841477 31.66460450981502 32.2107688721101 18.511228327233397 70-71 18.223583937937878 31.232843004929585 32.83194762262709 17.711625434505443 72-73 18.512515373924472 31.42541784107269 31.461811664817557 18.60025512018528 74-75 17.87945738949828 31.886597001363015 31.269215291750502 18.964730317388202 76-77 17.808470231043117 31.528543704643603 32.39078901052575 18.272197053787533 78-79 17.1714958702872 31.506367841143245 32.191979598818996 19.130156689750557 80-81 18.13452994887594 31.956872414138633 30.911443285106323 18.997154351879107 82-83 18.637008608645402 31.248276946991492 31.277870625781702 18.836843818581404 84-85 17.523200847301435 31.207430501294052 31.370245286496395 19.89912336490812 86-87 18.14899097277711 31.461380027548426 31.74418489945552 18.645444100218945 88-89 18.45140238260167 30.7624803068241 32.018481727536795 18.767635583037436 90-91 18.003222318136913 30.21951652762499 32.610389327083986 19.166871827154107 92-93 18.9297812750413 31.129240240157586 31.508816355061803 18.432162129739314 94-95 18.49501722361101 29.667702419612336 32.306042930531866 19.531237426244786 96-97 19.09862991373185 29.929666102133893 31.312520534165998 19.65918344996826 98-99 18.30473218928757 30.299203984127427 31.464060957647888 19.93200286893711 100-101 18.598044884404853 30.083492591758286 31.786355298712053 19.532107225124804 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2013.0 1 1241.5 2 997.5 3 1362.0 4 869.0 5 897.5 6 2080.0 7 3219.0 8 3687.5 9 3492.5 10 2589.5 11 1989.5 12 2043.5 13 2103.0 14 2006.0 15 1932.5 16 1951.0 17 1959.0 18 1971.0 19 2111.0 20 2460.0 21 3008.0 22 3601.0 23 4117.0 24 4473.0 25 5806.5 26 9738.0 27 13768.0 28 13825.0 29 15398.5 30 22109.5 31 27800.5 32 35870.5 33 46065.0 34 53575.5 35 60481.5 36 68662.0 37 72430.0 38 65378.5 39 57345.0 40 50184.5 41 42924.5 42 35559.0 43 35097.5 44 36150.0 45 30760.5 46 23276.0 47 16918.5 48 16887.5 49 14529.0 50 10319.0 51 6776.5 52 3934.0 53 2914.5 54 2269.0 55 1814.5 56 1442.0 57 1229.5 58 1189.0 59 1121.5 60 1009.0 61 995.0 62 945.0 63 796.5 64 687.0 65 611.0 66 477.0 67 473.0 68 384.0 69 175.5 70 122.0 71 97.5 72 73.0 73 68.0 74 56.0 75 47.0 76 27.0 77 17.0 78 16.0 79 11.5 80 11.0 81 7.5 82 4.0 83 4.5 84 3.5 85 3.0 86 4.0 87 3.5 88 2.5 89 3.0 90 4.0 91 4.5 92 4.0 93 3.0 94 4.0 95 6.0 96 6.5 97 5.5 98 3.5 99 2.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050005165822915594 2 0.011364810414298997 3 0.009505114164686435 4 0.004959189998966836 5 0.0079553672900093 6 0.00661225333195578 7 0.005992354582084926 8 0.011571443330922616 9 0.01766711437131935 10-11 0.002996177291042463 12-13 0.010744911664428144 14-15 0.009660088852154148 16-17 0.021386506870544476 18-19 0.011984709164169853 20-21 0.004597582394875503 22-23 0.011106519268519474 24-25 0.02458931707821056 26-27 0.007542101456762062 28-29 0.020198367599958675 30-31 0.016324000413265833 32-33 0.014309329476185555 34-35 0.2734270069222027 36-37 0.4809897716706271 38-39 0.47706374625477843 40-41 0.4986568860419465 42-43 0.4893067465647278 44-45 0.504907531769811 46-47 0.5121396838516376 48-49 0.5185969624961256 50-51 0.529806798222957 52-53 0.5177704308296311 54-55 0.5046492406240315 56-57 0.4978820126046079 58-59 0.4960223163549954 60-61 0.4919929744808348 62-63 0.4920962909391466 64-65 0.5080586837483211 66-67 0.4814546957330303 68-69 0.5074387849984503 70-71 0.4893067465647278 72-73 0.4984502531253229 74-75 0.5127079243723526 76-77 0.49932844302097323 78-79 0.49783035437545203 80-81 0.5011364810414299 82-83 0.5017563797913007 84-85 0.5003099493749354 86-87 0.5029961772910424 88-89 0.5168922409339808 90-91 0.5412749250955677 92-93 0.5285153424940593 94-95 0.49927678479181736 96-97 0.47179460688087616 98-99 0.4625477838619692 100-101 0.4732926955263974 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 967900.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.08334404259075 #Duplication Level Percentage of deduplicated Percentage of total 1 71.3975348795901 10.055160475021406 2 9.300570512115806 2.6196626862900274 3 4.019641907430309 1.6982999973107027 4 2.4105913661164995 1.3579675022047 5 1.608281177073088 1.1324988566971559 6 1.146397920728266 0.9687069796396108 7 0.925624886822707 0.9125125614855819 8 0.6935369515159867 0.7813855595559378 9 0.6267216618042664 0.7943703103920322 >10 5.5117550820861405 16.530354549631 >50 1.0591026968207151 10.478258822450089 >100 1.1573170645535704 33.72993743431126 >500 0.10554379816068025 10.234343132549984 >1k 0.03591420909634259 7.340179732880435 >5k 0.0014658860855650037 1.3663613995800636 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7611 0.7863415642111787 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5626 0.5812583944622378 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4295 0.4437441884492199 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2454 0.25353858869717943 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 2202 0.22750284120260358 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 2153 0.2224403347453249 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 2089 0.21582808141336912 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 2055 0.21231532183076762 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 2019 0.2085959293315425 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1998 0.20642628370699453 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 1812 0.18720942246099803 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1727 0.17842752350449426 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1709 0.1765678272548817 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1661 0.17160863725591485 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1639 0.16933567517305506 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 1624 0.16778592829837793 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 1534 0.15848744705031512 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 1523 0.1573509660088852 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1474 0.15228845955160658 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 1442 0.14898233288562868 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 1384 0.14298997830354376 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1362 0.14071701622068394 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 1279 0.13214175018080382 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1277 0.13193511726418017 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1252 0.12935220580638496 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1247 0.12883562351482591 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 1245 0.1286289905982023 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 1225 0.1265626614319661 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1215 0.12552949684884804 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 1214 0.1254261803905362 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1212 0.12521954747391262 No Hit CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA 1195 0.12346316768261183 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 1190 0.12294658539105278 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1181 0.12201673726624652 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 1163 0.12015704101663396 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1157 0.1195371422667631 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 1150 0.11881392705858043 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 1117 0.11540448393429073 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 1106 0.11426800289286083 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1100 0.11364810414298998 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1096 0.11323483830974275 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 1090 0.11261493955987188 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 1075 0.11106519268519474 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 1068 0.11034197747701209 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 1030 0.10641595206116335 No Hit CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA 1022 0.10558942039466887 No Hit CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC 1019 0.10527947101973345 No Hit CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG 1016 0.10496952164479802 No Hit CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT 1007 0.10403967351999173 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 1005 0.10383304060336812 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 1001 0.10341977477012088 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 996 0.10290319247856185 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 992 0.10248992664531459 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 985 0.10176671143713194 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 972 0.10042359747907842 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 970 0.1002169645624548 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.0331645831180907E-4 0.0 8 0.0 0.0 0.0 1.0331645831180907E-4 0.0 9 0.0 0.0 0.0 1.0331645831180907E-4 0.0 10-11 0.0 0.0 0.0 3.099493749354272E-4 0.0 12-13 0.0 0.0 0.0 6.71556979026759E-4 0.0 14-15 0.0 0.0 0.0 9.815063539621862E-4 0.0 16-17 0.0 0.0 0.0 0.003202810207666081 0.0 18-19 0.0 0.0 0.0 0.005320797603058167 0.0 20-21 0.0 0.0 0.0 0.008575266039880153 0.0 22-23 0.0 0.0 0.0 0.017822089058787065 0.0 24-25 0.0 0.0 0.0 0.027120570306849882 0.0 26-27 0.0 0.0 0.0 0.031046595722698626 0.0 28-29 0.0 0.0 0.0 0.036522368013224504 0.0 30-31 0.0 0.0 0.0 0.053982849467920244 0.0 32-33 0.0 0.0 0.0 0.0842545717532803 0.0 34-35 0.0 0.0 0.0 0.13255501601405104 0.0 36-37 0.0 0.0 0.0 0.20487653683231738 0.0 38-39 0.0 0.0 0.0 0.3109308812893894 0.0 40-41 0.0 0.0 0.0 0.43744188449219956 0.0 42-43 0.0 0.0 0.0 0.5480421531149913 0.0 44-45 0.0 0.0 0.0 0.6690257257981196 0.0 46-47 0.0 0.0 0.0 0.8280297551399938 0.0 48-49 0.0 0.0 0.0 1.0003099493749354 0.0 50-51 0.0 0.0 0.0 1.1745531563178013 0.0 52-53 0.0 0.0 0.0 1.3422874263870233 0.0 54-55 0.0 0.0 0.0 1.5162206839549541 0.0 56-57 0.0 0.0 0.0 1.7161896890174604 0.0 58-59 0.0 0.0 0.0 1.9395598718875915 0.0 60-61 0.0 0.0 0.0 2.1778592829837793 0.0 62-63 0.0 0.0 0.0 2.4008162000206634 0.0 64-65 0.0 0.0 0.0 2.6323483830974275 0.0 66-67 0.0 0.0 0.0 2.9094947825188555 0.0 68-69 0.0 0.0 0.0 3.2124702965182355 0.0 70-71 0.0 0.0 0.0 3.5561008368633122 0.0 72-73 0.0 0.0 0.0 3.8779316045045977 0.0 74-75 0.0 0.0 0.0 4.206322967248683 0.0 76-77 0.0 0.0 0.0 4.578623824775287 0.0 78-79 0.0 0.0 0.0 4.998656886041946 0.0 80-81 0.0 0.0 0.0 5.459344973654304 0.0 82-83 0.0 0.0 0.0 5.913059200330613 0.0 84-85 0.0 0.0 0.0 6.373850604401281 0.0 86-87 0.0 0.0 0.0 6.8654303130488685 0.0 88-89 0.0 0.0 0.0 7.39544374418845 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 245 0.0 46.370293 9 GTATGGT 85 7.4232958E-9 44.565662 1 GGGATGC 345 0.0 43.90617 7 GGTATCA 3610 0.0 42.366695 1 GGATGCC 365 0.0 41.50035 8 TAGGCAT 270 0.0 38.570347 5 GTACCAT 50 0.0016433176 37.869072 6 GTATCAA 6005 0.0 36.66648 1 AAGAGTG 95 8.9360765E-7 34.87941 7 CATGGGT 540 0.0 34.187355 4 CATGGGG 1775 0.0 34.135498 4 TACCTGG 85 1.6691716E-5 33.419064 2 TGGGTAT 255 0.0 33.413887 6 GCGCTTC 50 1.2860146E-6 33.295403 62-63 TCGCGGA 50 1.2860146E-6 33.295403 82-83 TACAGGG 130 8.554707E-9 32.776394 2 AATGTTC 130 8.56744E-9 32.77131 8 CTTTAGG 325 0.0 32.048027 2 ATGTATT 90 2.4649302E-5 31.56734 1 GAGTGCA 105 1.953169E-6 31.55756 9 >>END_MODULE