##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936370_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 967900 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.42843165616283 34.0 31.0 34.0 31.0 34.0 2 32.56154664738093 34.0 31.0 34.0 31.0 34.0 3 32.63779109412129 34.0 31.0 34.0 31.0 34.0 4 36.15718152701725 37.0 35.0 37.0 35.0 37.0 5 36.1430168405827 37.0 35.0 37.0 35.0 37.0 6 36.19221820435995 37.0 37.0 37.0 35.0 37.0 7 36.14374212212005 37.0 36.0 37.0 35.0 37.0 8 36.13246513069532 37.0 36.0 37.0 35.0 37.0 9 37.87711540448394 39.0 38.0 39.0 35.0 39.0 10-11 37.82716447980163 39.0 38.0 39.0 35.0 39.0 12-13 37.804224093398076 39.0 38.0 39.0 35.0 39.0 14-15 39.08240933980783 40.5 38.0 41.0 36.0 41.0 16-17 39.02992974480835 40.0 38.0 41.0 35.5 41.0 18-19 39.1662000206633 40.0 38.5 41.0 36.0 41.0 20-21 39.1443635706168 40.0 39.0 41.0 36.0 41.0 22-23 38.998869717946064 40.0 38.5 41.0 35.0 41.0 24-25 39.1308694079967 40.5 39.0 41.0 35.0 41.0 26-27 39.00313617109205 40.5 38.5 41.0 35.0 41.0 28-29 38.907071494989154 40.0 38.0 41.0 35.0 41.0 30-31 38.734002996177296 40.0 38.0 41.0 35.0 41.0 32-33 38.49505165822916 40.0 38.0 41.0 35.0 41.0 34-35 38.311810104349625 40.0 38.0 41.0 34.0 41.0 36-37 38.125680338877984 40.0 38.0 41.0 34.0 41.0 38-39 37.946880359541275 40.0 37.5 41.0 34.0 41.0 40-41 37.820038227089576 40.0 37.5 41.0 33.0 41.0 42-43 37.727755966525464 40.0 38.0 41.0 33.0 41.0 44-45 37.47983727657816 40.0 38.0 41.0 33.0 41.0 46-47 37.11728329372869 40.0 38.0 41.0 32.5 41.0 48-49 36.79056979026759 40.0 37.0 41.0 31.0 41.0 50-51 36.59782002272962 40.0 37.0 41.0 31.0 41.0 52-53 36.44632916623618 40.0 37.0 41.0 30.0 41.0 54-55 36.31317336501705 40.0 37.0 41.0 30.0 41.0 56-57 36.126862795743364 40.0 36.5 41.0 29.5 41.0 58-59 35.94935065605951 40.0 36.0 41.0 28.5 41.0 60-61 35.77307366463478 39.5 36.0 41.0 28.0 41.0 62-63 35.51336243413576 39.0 35.5 41.0 28.0 41.0 64-65 35.21152908358302 39.0 35.0 40.5 27.0 41.0 66-67 34.91092003306127 38.5 35.0 40.0 26.0 41.0 68-69 34.556065192685196 38.0 35.0 40.0 26.0 41.0 70-71 34.22161690257258 37.0 35.0 39.5 26.0 41.0 72-73 33.81131366876744 37.0 34.0 39.0 25.5 41.0 74-75 33.33715259840892 36.0 34.0 39.0 24.5 40.0 76-77 32.56091073458002 35.0 33.0 37.5 22.5 39.0 78-79 32.558460584771154 35.0 34.0 37.0 22.0 39.0 80-81 32.32631418534973 35.0 34.0 37.0 22.0 39.0 82-83 31.973162516788925 35.0 33.0 36.5 21.5 39.0 84-85 31.610341977477013 35.0 33.0 36.0 20.0 37.0 86-87 31.212183076764127 35.0 33.0 36.0 18.5 37.0 88-89 30.684320694286598 35.0 32.0 35.0 7.0 36.5 90-91 30.457413989048455 35.0 32.0 35.0 4.5 36.0 92-93 30.182317388159934 35.0 32.0 35.0 2.0 36.0 94-95 30.03535075937597 34.5 32.0 35.0 2.0 36.0 96-97 29.832433102593242 34.0 31.5 35.0 2.0 35.0 98-99 29.51380979440025 34.0 31.0 35.0 2.0 35.0 100-101 26.52713038537039 31.5 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11054650968204527 1101 2 0.1672786834733131 1101 3 0.15013313572359266 1101 4 0.15584399192460552 1101 5 0.03177539968309162 1101 6 0.025902147080010707 1101 7 0.14950299920926824 1101 8 0.06949655669206578 1101 9 0.20517294253781415 1101 10-11 0.16092289647341573 1101 12-13 0.25796447177265946 1101 14-15 0.35983388218599544 1101 16-17 0.3565848802732816 1101 18-19 0.27453674969923725 1101 20-21 0.2901665897775416 1101 22-23 0.31802849769871244 1101 24-25 0.40296008683386475 1101 26-27 0.3302587904574139 1101 28-29 0.26988334659155555 1101 30-31 0.29836121157559603 1101 32-33 0.477850763702925 1101 34-35 0.3803412296009938 1101 36-37 0.3252088646665854 1101 38-39 0.39576943578047974 1101 40-41 0.46637100598135106 1101 42-43 0.3630901441453389 1101 44-45 0.48145381009682353 1101 46-47 0.27555945730542675 1101 48-49 0.1540556789586205 1101 50-51 0.031155844346876904 1101 52-53 0.14910274398187084 1101 54-55 0.08958419541657037 1101 56-57 0.049318799252510814 1101 58-59 -0.10111009553361328 1101 60-61 0.0132659278655467 1101 62-63 0.11748315056985348 1101 64-65 0.20105428455035224 1101 66-67 0.19179683716455287 1101 68-69 0.176995679163241 1101 70-71 0.24830690527065968 1101 72-73 0.13460214143179172 1101 74-75 0.008484952792827016 1101 76-77 -0.109844937264711 1101 78-79 -0.11776444153623089 1101 80-81 0.11209710958479491 1101 82-83 -0.04419919867649469 1101 84-85 0.1598994646159575 1101 86-87 -0.0010195757262465577 1101 88-89 0.07452134711387615 1101 90-91 0.4037339757819005 1101 92-93 0.46763888650621865 1101 94-95 0.4951693682218874 1101 96-97 0.33341924246273535 1101 98-99 0.3629377174751447 1101 100-101 0.7460237942001022 1102 1 0.06952297599362822 1102 2 0.1425343298199806 1102 3 0.1772782733063707 1102 4 0.15087014924786502 1102 5 0.19875553276143876 1102 6 0.1895047454268024 1102 7 0.1457366607603774 1102 8 0.15520090781659235 1102 9 0.15618051314677928 1102 10-11 0.22462585675537383 1102 12-13 0.3743495455031294 1102 14-15 0.37353131070774737 1102 16-17 0.29223865530449444 1102 18-19 0.3031557062512178 1102 20-21 0.2133762750994066 1102 22-23 0.3647220855983804 1102 24-25 0.264309678273861 1102 26-27 0.08738922936307603 1102 28-29 0.1931728473106844 1102 30-31 0.10077780053421037 1102 32-33 0.016889185728807377 1102 34-35 -0.054542050258653774 1102 36-37 -0.10623761160216816 1102 38-39 -0.12129367370095423 1102 40-41 -0.06599769373890041 1102 42-43 -0.0866237848268625 1102 44-45 -0.13106140224373775 1102 46-47 -0.17092708471223972 1102 48-49 -0.0612111889587581 1102 50-51 -0.14393701019944416 1102 52-53 -0.09376997811830279 1102 54-55 -0.1871097085836837 1102 56-57 -0.11176526769759221 1102 58-59 -0.19256510651810288 1102 60-61 -0.059946128210697225 1102 62-63 0.30333290796265544 1102 64-65 0.14166056546893913 1102 66-67 -0.0086867967277513 1102 68-69 -0.02199358922195671 1102 70-71 -0.12905713196599322 1102 72-73 -0.13846687913055433 1102 74-75 -0.08702404817127984 1102 76-77 -0.33333595210562805 1102 78-79 -0.30888122489668746 1102 80-81 -0.23322065052646934 1102 82-83 -0.14504002622781798 1102 84-85 -0.016784166115339616 1102 86-87 -0.12406725949922404 1102 88-89 0.02475368104253306 1102 90-91 0.13413969088044553 1102 92-93 0.17072560870121833 1102 94-95 0.26235891793660215 1102 96-97 -0.11709521123643185 1102 98-99 -0.07379949230501026 1102 100-101 0.3742484152745291 1103 1 0.016416373551159325 1103 2 0.16539595585997802 1103 3 0.2747974919119258 1103 4 0.08434093210195215 1103 5 0.19045686590015265 1103 6 0.19920831668160588 1103 7 0.20540252462453168 1103 8 0.17211145615503654 1103 9 0.18106435005623212 1103 10-11 0.34784996029257087 1103 12-13 0.2834078338308572 1103 14-15 0.4548819651134295 1103 16-17 0.4505534390343655 1103 18-19 0.48666754150641367 1103 20-21 0.4986935037278357 1103 22-23 0.5170356164697907 1103 24-25 0.5076333414860414 1103 26-27 0.5181475128253439 1103 28-29 0.5135990016433851 1103 30-31 0.4257060348552315 1103 32-33 0.6098485468926285 1103 34-35 0.5753112558280478 1103 36-37 0.537065677455395 1103 38-39 0.632677765991545 1103 40-41 0.7819776626567645 1103 42-43 0.6414192624168535 1103 44-45 0.5417768087230215 1103 46-47 0.5517958754395664 1103 48-49 0.5912254991159784 1103 50-51 0.49702477153558533 1103 52-53 0.4553925528990703 1103 54-55 0.5401867931435547 1103 56-57 0.6603513332852273 1103 58-59 0.637096317604886 1103 60-61 0.5416403190232799 1103 62-63 0.5746284609233001 1103 64-65 0.5747405902828362 1103 66-67 0.7094915105753188 1103 68-69 0.667574235426514 1103 70-71 0.5737143612466795 1103 72-73 0.49840334662724217 1103 74-75 0.5862809438006948 1103 76-77 0.42300132225820875 1103 78-79 0.38583943632065143 1103 80-81 0.48924991338147095 1103 82-83 0.35968099989975855 1103 84-85 0.31068627615585953 1103 86-87 0.29476302075183725 1103 88-89 0.3820905053504191 1103 90-91 0.6362804975753278 1103 92-93 0.6390514005564221 1103 94-95 0.5344724780008292 1103 96-97 0.5046053920818174 1103 98-99 0.5191401028879064 1103 100-101 0.8939474859698073 1104 1 0.008602979815606204 1104 2 0.09649108936262252 1104 3 -0.15173458532970585 1104 4 -0.06706216122406516 1104 5 -0.013888261882577524 1104 6 -0.02731861264321367 1104 7 0.03150732477096341 1104 8 0.08014249237348992 1104 9 0.12754174498152793 1104 10-11 0.11746066338361061 1104 12-13 0.1523390119030239 1104 14-15 0.14412510694425862 1104 16-17 0.21235861326275085 1104 18-19 0.16686965444026214 1104 20-21 0.07880938905513091 1104 22-23 0.0753615139232835 1104 24-25 0.08089749253736045 1104 26-27 0.21147752861229208 1104 28-29 0.2931559090037652 1104 30-31 0.25167784715074504 1104 32-33 0.2160836751329569 1104 34-35 0.19341304730309616 1104 36-37 0.2550470648761731 1104 38-39 0.1667197958723463 1104 40-41 0.29915311510145415 1104 42-43 0.4941509472728214 1104 44-45 0.3931900548849754 1104 46-47 0.358517338820711 1104 48-49 0.4775058718251515 1104 50-51 0.3614615322375343 1104 52-53 0.2092577804369924 1104 54-55 0.21921132010720612 1104 56-57 0.22918041100733433 1104 58-59 0.3858701124203634 1104 60-61 0.28569233014278694 1104 62-63 0.1464175908109553 1104 64-65 0.04137565446020375 1104 66-67 0.10300613637896561 1104 68-69 -0.047249763017674695 1104 70-71 0.08640826611317465 1104 72-73 0.2894200887966889 1104 74-75 0.11808219989239177 1104 76-77 0.21692449245978196 1104 78-79 -0.03857842008679313 1104 80-81 0.006608402868280905 1104 82-83 -0.07748224054228103 1104 84-85 0.1377677505192345 1104 86-87 0.17021989836869622 1104 88-89 0.28285511399434426 1104 90-91 0.4557373351528362 1104 92-93 0.35320473983846057 1104 94-95 0.2744683596673809 1104 96-97 0.09500424123641338 1104 98-99 0.10610414754894393 1104 100-101 0.26734019194367775 1105 1 0.0766885924043379 1105 2 0.12511059773046895 1105 3 -0.001923247930399441 1105 4 0.09814559486052588 1105 5 -0.007739852649805812 1105 6 0.12152234538587692 1105 7 0.020656719085820896 1105 8 0.01837771179818759 1105 9 0.11533848926518431 1105 10-11 0.18412278557388362 1105 12-13 0.13231457865938268 1105 14-15 0.17846865064674944 1105 16-17 0.10259213251863031 1105 18-19 0.09100897739348568 1105 20-21 0.14463211972406498 1105 22-23 0.02082579233394455 1105 24-25 0.223873402802667 1105 26-27 0.22707042907088493 1105 28-29 0.18943157694154422 1105 30-31 0.2240855241079771 1105 32-33 0.35729860079734976 1105 34-35 0.3270547367255574 1105 36-37 0.2920644982895695 1105 38-39 0.2639441900869599 1105 40-41 0.43978795172083807 1105 42-43 0.4038194544524245 1105 44-45 0.5951024774784983 1105 46-47 0.6505173227813827 1105 48-49 0.6340944937928867 1105 50-51 0.4725191789205283 1105 52-53 0.42911821154740437 1105 54-55 0.5308461152916593 1105 56-57 0.4128233702099706 1105 58-59 0.4711518900056717 1105 60-61 0.5128412994154345 1105 62-63 0.6959182998829334 1105 64-65 0.4967408766516783 1105 66-67 0.4117879360183494 1105 68-69 0.43454158937840504 1105 70-71 0.4083911013585251 1105 72-73 0.2968102093723459 1105 74-75 0.15105504931919 1105 76-77 0.18160923277636698 1105 78-79 0.1781483272309643 1105 80-81 0.3053716369850008 1105 82-83 0.269118958932161 1105 84-85 0.22593794383324095 1105 86-87 0.27653859180500007 1105 88-89 0.31436637961765257 1105 90-91 0.597031120848694 1105 92-93 0.4911503157238677 1105 94-95 0.4901358430031877 1105 96-97 0.33245981563818106 1105 98-99 0.46333608024792383 1105 100-101 0.6440064442610272 1106 1 0.0036010084972915024 1106 2 -0.00606392502070463 1106 3 0.1164164773900751 1106 4 -0.027743321394673615 1106 5 -0.07147597891012225 1106 6 -0.023006934606620177 1106 7 0.020386965499859855 1106 8 0.02622793209695118 1106 9 -0.02592638138992953 1106 10-11 0.06657277364999459 1106 12-13 0.10572430308067737 1106 14-15 0.1819949946255761 1106 16-17 0.05463854577330807 1106 18-19 0.03426298753465318 1106 20-21 0.11096178988189109 1106 22-23 0.07149255282794797 1106 24-25 -0.04509828870870081 1106 26-27 4.0509162478485905E-4 1106 28-29 0.09227923653597969 1106 30-31 0.15747007152792492 1106 32-33 0.21118989401168164 1106 34-35 0.2637857924003484 1106 36-37 0.12115605422042819 1106 38-39 0.22120583933693183 1106 40-41 0.05370606391488053 1106 42-43 0.22567066775715006 1106 44-45 0.11133069683116759 1106 46-47 0.04951219746325819 1106 48-49 0.11106965691561754 1106 50-51 0.2925603065687099 1106 52-53 0.15839312172728626 1106 54-55 0.32922681188143343 1106 56-57 0.33896757500375685 1106 58-59 0.3218002759800527 1106 60-61 0.29725540635514136 1106 62-63 0.4478759681172946 1106 64-65 0.41870390086304354 1106 66-67 0.3922171178830851 1106 68-69 0.4758080238224167 1106 70-71 0.43154495082038835 1106 72-73 0.40204342894003275 1106 74-75 0.6660967438408463 1106 76-77 0.524286204811915 1106 78-79 0.39454543578528245 1106 80-81 0.4975104701639097 1106 82-83 0.5055540675568508 1106 84-85 0.5436548902226512 1106 86-87 0.46351887633639066 1106 88-89 0.5826421850666748 1106 90-91 0.6191821906757795 1106 92-93 0.5278622149845837 1106 94-95 0.4464044862990306 1106 96-97 0.4487138375077713 1106 98-99 0.36456521832657174 1106 100-101 0.3822614122228991 1107 1 -0.059745466037789186 1107 2 0.04141551762122475 1107 3 0.08337765135525643 1107 4 0.046527863278647885 1107 5 0.04015070935998466 1107 6 0.056286782615572406 1107 7 0.0835663342141757 1107 8 0.14532491897847422 1107 9 0.13253004209097696 1107 10-11 0.08388426825782602 1107 12-13 0.08182549701835029 1107 14-15 0.16374236003520082 1107 16-17 0.2679517996712164 1107 18-19 0.15609984145341826 1107 20-21 0.11606119110830804 1107 22-23 0.15384698026324628 1107 24-25 0.10682964456661637 1107 26-27 0.2909666874987451 1107 28-29 0.3431197788144189 1107 30-31 0.10975133335065834 1107 32-33 0.11747293918774204 1107 34-35 0.24665811419536254 1107 36-37 0.29206988242762577 1107 38-39 0.2783339720697455 1107 40-41 0.27156459613755857 1107 42-43 0.3274468021048804 1107 44-45 0.4378715127621078 1107 46-47 0.5906342662600537 1107 48-49 0.33567191164103605 1107 50-51 0.41494860969599046 1107 52-53 0.49909114279331135 1107 54-55 0.6332578053322138 1107 56-57 0.5814922632177186 1107 58-59 0.33389405844035736 1107 60-61 0.4632917498658955 1107 62-63 0.5914613103968094 1107 64-65 0.5688284281206748 1107 66-67 0.4906090251487143 1107 68-69 0.6864001265350126 1107 70-71 0.8387183358094745 1107 72-73 0.6783221561016788 1107 74-75 0.512418878410493 1107 76-77 0.4090500308133116 1107 78-79 0.4807947656909377 1107 80-81 0.501693463069266 1107 82-83 0.7322638608937382 1107 84-85 0.6767780712757876 1107 86-87 0.6167609297861922 1107 88-89 0.6148133976800914 1107 90-91 0.434211420825946 1107 92-93 0.47706204547244013 1107 94-95 0.5390345848647264 1107 96-97 0.22413230390412409 1107 98-99 0.3266402180926242 1107 100-101 0.3241132655601362 1108 1 -0.08924576171330756 1108 2 0.008474980126273124 1108 3 0.11562404931675019 1108 4 0.05858711005477346 1108 5 0.01704867973151636 1108 6 0.015657412518649494 1108 7 0.053132059202944504 1108 8 -0.06230655510994154 1108 9 -0.07960509050617048 1108 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-1.7220953153300123 2316 100-101 -1.9678978850900144 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 7.0 5 21.0 6 50.0 7 125.0 8 236.0 9 429.0 10 822.0 11 1592.0 12 2827.0 13 4538.0 14 4830.0 15 6380.0 16 8625.0 17 12281.0 18 10672.0 19 4736.0 20 3337.0 21 3297.0 22 3353.0 23 3661.0 24 4228.0 25 4935.0 26 5755.0 27 7102.0 28 8856.0 29 11354.0 30 14099.0 31 17402.0 32 21728.0 33 27954.0 34 37552.0 35 59761.0 36 84953.0 37 180935.0 38 330559.0 39 78896.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.238155177968885 23.995988616455996 14.385036876342264 21.380819329232853 2 14.208652609061833 24.583826348450167 40.68028335667081 20.52723768581718 3 15.204463270999069 31.836966628783962 32.674553156317806 20.284016943899164 4 9.512966215518132 19.92292592209939 43.82560181836966 26.738506044012812 5 8.77786961462961 42.97127802458932 37.69325343527224 10.557598925508833 6 23.630127079243724 44.77508007025519 19.55563591280091 12.039156937700175 7 23.772290525880774 36.54313462134518 25.72476495505734 13.959809897716708 8 23.9099080483521 36.99483417708441 22.97820022729621 16.11705754726728 9 26.640148775699966 15.92457898543238 24.512656266143196 32.92261597272446 10-11 21.010388523548524 29.807623760841828 31.592424798144425 17.58956291746522 12-13 21.86367393325757 29.272497158797396 31.087818989565037 17.776009918379998 14-15 20.16499638392396 30.33980783138754 28.92762682095258 20.567568963735923 16-17 17.05548334838319 33.39456593387292 30.62236153959008 18.927589178153806 18-19 16.624547990494886 32.07252815373489 34.23179047422254 17.071133381547682 20-21 17.534826704632042 31.319315693416517 34.28997535384614 16.855882248105306 22-23 18.08169034165755 30.662496810101054 33.63630916035661 17.619503687884787 24-25 17.595147334820776 31.63091931286972 33.73686111043667 17.037072241872835 26-27 16.611227201930006 31.69751181069821 34.68184084163978 17.009420145732005 28-29 17.005443399135935 30.088043894347326 35.366234807481796 17.540277899034944 30-31 17.29328940258522 30.947982564080313 34.594689256080514 17.164038777253957 32-33 16.424308453840872 32.23842076361305 34.56416074008754 16.773110042458534 34-35 16.965377013543993 31.164975518735222 34.741750810790144 17.12789665693064 36-37 17.850148698289438 30.328458688117934 33.441850109438015 18.379542504154614 38-39 16.561653933754418 32.161275175031264 33.925251459734675 17.351819431479644 40-41 16.503287050477898 31.199083779235014 35.44373373393023 16.853895436356854 42-43 16.897553576852896 31.243865344439598 33.54259181577539 18.31598926293211 44-45 16.795543120229127 31.73889189669495 33.57853266821787 17.887032314858057 46-47 17.30005068449907 31.256526081333895 33.40991979862599 18.033503435541046 48-49 17.25290077384969 31.69957603080932 32.958773826954854 18.088749368386136 50-51 17.347456974779234 31.421778642710755 32.322656737961644 18.908107644548362 52-53 17.177521096316166 32.03777963532588 32.70360386144987 18.081095406908084 54-55 18.11755018374965 31.48478189221329 32.17850526866089 18.219162655376163 56-57 18.055331696753292 32.20394845599418 31.30853046683859 18.432189380413938 58-59 17.066069486740385 31.39699292011169 32.415453322071635 19.12148427107629 60-61 18.147474284108426 32.852387269548835 30.917186357042787 18.08295208929996 62-63 17.689637359231327 32.55568757103007 32.16711437131935 17.587560698419257 64-65 17.554224939933743 31.440882456106817 32.19329272608082 18.81159987787862 66-67 17.603574749457586 32.340944312428974 31.387281743981816 18.668199194131624 68-69 17.241553879533008 31.742070461824568 32.20291352412439 18.813462134518026 70-71 17.919994544871276 31.169655019932403 32.819366533508486 18.090983901687835 72-73 18.121620825786746 31.38647782724458 31.37816065471129 19.113740692257384 74-75 17.408513276164893 31.802200640562038 31.33991114784585 19.449374935427212 76-77 17.23899829757587 31.572125794080698 32.261946602165374 18.926929306178057 78-79 16.59918128074334 31.47171922769828 32.15138239029617 19.77771710126221 80-81 17.40898071104485 31.927795178251973 30.931964576388427 19.731259534314752 82-83 17.77384027275545 31.34481867961566 31.265110032028105 19.616231015600786 84-85 16.654122268364716 31.18508144732567 31.323061218902247 20.83773506540737 86-87 17.190031178428747 31.47428245132379 31.72260301605265 19.613083354194814 88-89 17.41211334392862 30.856555132044367 31.85404698029131 19.8772845437357 90-91 16.813121890547265 30.19330431177446 32.605410447761194 20.388163349917082 92-93 17.609982077896642 31.1118180701848 31.501503353075798 19.776696498842757 94-95 17.05889129449883 29.626972036205036 32.37685799789539 20.93727867140074 96-97 17.523045355077212 29.978230637459006 31.43937124047138 21.059352766992397 98-99 16.725808776313674 30.298031805006165 31.541901698219743 21.434257720460415 100-101 16.652796353321566 29.97341135836602 32.120339561801416 21.253452726511 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2097.0 1 1275.0 2 899.0 3 1279.5 4 856.5 5 883.5 6 2074.0 7 3133.0 8 3395.5 9 3084.0 10 2226.5 11 1573.5 12 1477.5 13 1464.5 14 1452.5 15 1508.5 16 1621.5 17 1742.5 18 1890.0 19 2114.0 20 2531.5 21 3103.5 22 3669.0 23 4233.5 24 4632.0 25 5928.0 26 9893.0 27 14011.0 28 14006.5 29 15491.0 30 22318.0 31 27943.5 32 35927.0 33 46323.0 34 53556.0 35 60442.0 36 68859.5 37 72518.5 38 65465.5 39 57668.0 40 51078.0 41 43645.0 42 35952.0 43 35759.0 44 36630.5 45 30813.0 46 23370.0 47 17072.0 48 16998.0 49 14686.5 50 10544.5 51 6892.5 52 3608.5 53 2395.0 54 1868.5 55 1597.0 56 1363.5 57 1183.5 58 1171.0 59 1115.5 60 985.5 61 961.0 62 893.0 63 739.5 64 648.0 65 567.5 66 426.0 67 437.5 68 359.5 69 160.5 70 88.0 71 73.5 72 73.0 73 70.0 74 61.0 75 41.0 76 16.5 77 11.5 78 10.0 79 5.0 80 3.0 81 2.0 82 2.0 83 1.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2734786651513586 2 0.02221303853703895 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.165822915590454E-4 12-13 0.0 14-15 0.0 16-17 6.198987498708544E-4 18-19 0.0 20-21 5.165822915590453E-5 22-23 1.0331645831180906E-4 24-25 0.002014670937080277 26-27 0.0028412026035747493 28-29 0.0031511519785101766 30-31 0.001343113958053518 32-33 0.0014980886455212316 34-35 0.0017563797913007543 36-37 5.165822915590453E-5 38-39 1.549746874677136E-4 40-41 0.0011364810414298998 42-43 0.003926025415848745 44-45 0.004959189998966836 46-47 0.015342494059303647 48-49 0.014567620621965078 50-51 0.006870544477735304 52-53 0.0079553672900093 54-55 7.232152081826635E-4 56-57 0.002892860832730654 58-59 0.0018080380204566588 60-61 0.001653063332988945 62-63 0.0 64-65 0.001084822812273995 66-67 0.0 68-69 0.0 70-71 3.6160760409133176E-4 72-73 0.0023762785411716087 74-75 0.0 76-77 0.19883252402107657 78-79 0.10579605331129248 80-81 0.0014980886455212316 82-83 0.0 84-85 4.6492406240314084E-4 86-87 0.12459964872404174 88-89 0.6030581671660296 90-91 0.32028102076660814 92-93 0.2986362227502841 94-95 0.002427936770327513 96-97 0.002686227916107036 98-99 0.003461101353445604 100-101 5.682405207149499E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 967900.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.878514558249707 #Duplication Level Percentage of deduplicated Percentage of total 1 69.01015967043607 8.887483459828477 2 9.870330494371771 2.5423038993300575 3 4.332555066863475 1.6739062050905935 4 2.589567722261713 1.3339914244288402 5 1.749217447077695 1.126366117886724 6 1.2602845345511446 0.9738355635452325 7 1.0016577981510968 0.9029905175111274 8 0.8027009946582995 0.8270077156502744 9 0.6599474200739787 0.7649228211391863 >10 6.118293251874732 16.94123424936963 >50 1.181960903025041 10.8391499127922 >100 1.259742590673229 33.7195048544675 >500 0.12348845255485504 10.902867095492207 >1k 0.038489907289824946 7.243931114545747 >5k 0.0016037461370760392 1.3205050489221974 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7251 0.7491476392189276 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5536 0.571959913214175 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4001 0.4133691497055481 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2485 0.25674139890484554 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 2180 0.22522987911974376 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 2068 0.21365843578882115 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 2064 0.21324516995557394 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 2039 0.2106622584977787 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 2004 0.20704618245686537 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1935 0.19991734683335055 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 1872 0.1934084099597066 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1853 0.19144539725178222 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1724 0.17811757412955884 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1706 0.17625787787994626 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 1697 0.17532802975514 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1597 0.1649963839239591 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 1529 0.15797086475875605 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 1508 0.15580121913420808 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 1455 0.1503254468436822 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 1442 0.14898233288562868 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1427 0.14743258601095155 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 1339 0.13834073767951235 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1290 0.1332782312222337 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1260 0.13017873747287942 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 1246 0.12873230705651412 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1244 0.12852567413989047 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1242 0.12831904122326687 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 1233 0.12738919309846058 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 1228 0.12687261080690154 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 1227 0.12676929434858972 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 1221 0.12614939559871888 No Hit CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA 1220 0.12604607914040708 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 1211 0.12511623101560077 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1172 0.12108688914144022 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1164 0.12026035747494576 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1148 0.11860729414195682 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 1147 0.11850397768364501 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 1123 0.11602438268416158 No Hit CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT 1108 0.11447463580948444 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 1091 0.1127182560181837 No Hit CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG 1087 0.11230499018493646 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1087 0.11230499018493646 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 1084 0.11199504081000104 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1082 0.11178840789337742 No Hit CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA 1081 0.11168509143506561 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 1076 0.11116850914350655 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 1059 0.1094121293522058 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 1036 0.1070358508110342 No Hit CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC 1032 0.10662258497778697 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1022 0.10558942039466887 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 999 0.10321314185349727 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 993 0.1025932431036264 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 983 0.10156007852050832 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 978 0.10104349622894929 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.0331645831180907E-4 0.0 8 0.0 0.0 0.0 1.0331645831180907E-4 0.0 9 0.0 0.0 0.0 1.0331645831180907E-4 0.0 10-11 0.0 0.0 0.0 3.099493749354272E-4 0.0 12-13 0.0 0.0 0.0 6.71556979026759E-4 0.0 14-15 0.0 0.0 0.0 9.815063539621862E-4 0.0 16-17 0.0 0.0 0.0 0.003202810207666081 0.0 18-19 0.0 0.0 0.0 0.0054757722905258805 0.0 20-21 0.0 0.0 0.0 0.00888521541481558 0.0 22-23 0.0 0.0 0.0 0.018596962496125632 0.0 24-25 0.0 0.0 0.0 0.02810207666081207 0.0 26-27 0.0 0.0 0.0 0.03228639322244034 0.0 28-29 0.0 0.0 0.0 0.038072114887901645 0.0 30-31 0.0 0.0 0.0 0.05573922925922099 0.0 32-33 0.0 0.0 0.0 0.08626924269036057 0.0 34-35 0.0 0.0 0.0 0.13493129455522265 0.0 36-37 0.0 0.0 0.0 0.20756276474842442 0.0 38-39 0.0 0.0 0.0 0.3153218307676413 0.0 40-41 0.0 0.0 0.0 0.44240107449116645 0.0 42-43 0.0 0.0 0.0 0.5540345076970761 0.0 44-45 0.0 0.0 0.0 0.6759479285050108 0.0 46-47 0.0 0.0 0.0 0.8368633123256535 0.0 48-49 0.0 0.0 0.0 1.009815063539622 0.0 50-51 0.0 0.0 0.0 1.1851947515239178 0.0 52-53 0.0 0.0 0.0 1.3541171608637257 0.0 54-55 0.0 0.0 0.0 1.5288769500981507 0.0 56-57 0.0 0.0 0.0 1.7297241450563074 0.0 58-59 0.0 0.0 0.0 1.9549540241760512 0.0 60-61 0.0 0.0 0.0 2.195371422667631 0.0 62-63 0.0 0.0 0.0 2.419929744808348 0.0 64-65 0.0 0.0 0.0 2.652081826634983 0.0 66-67 0.0 0.0 0.0 2.9294348589730346 0.0 68-69 0.0 0.0 0.0 3.2318937906808554 0.0 70-71 0.0 0.0 0.0 3.575834280400868 0.0 72-73 0.0 0.0 0.0 3.899318111375142 0.0 74-75 0.0 0.0 0.0 4.229362537452216 0.0 76-77 0.0 0.0 0.0 4.603058167166029 0.0 78-79 0.0 0.0 0.0 5.02205806384957 0.0 80-81 0.0 0.0 0.0 5.483675999586734 1.0331645831180907E-4 82-83 0.0 0.0 0.0 5.937235251575576 1.0331645831180907E-4 84-85 0.0 0.0 0.0 6.396735199917347 1.0331645831180907E-4 86-87 0.0 0.0 0.0 6.889864655439611 1.0331645831180907E-4 88-89 0.0 0.0 0.0 7.417811757412956 1.0331645831180907E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACACG 20 1.7344586E-5 95.10161 1 ACGCACC 20 0.0020852112 71.15303 7 ACTTGAC 35 3.4443456E-6 67.764786 8 CGCGAGT 30 1.2942578E-4 63.401066 1 CTAGACG 25 0.0050481763 56.92242 3 AGTTATC 25 0.0050481763 56.92242 8 CGAGTAC 35 2.8065377E-4 54.21183 3 CACCGTC 35 2.8065377E-4 54.21183 7 GTACAGG 100 1.2732926E-11 47.5508 1 CGTCTAG 50 2.8088558E-5 47.5508 1 CATGGGT 505 0.0 45.087063 4 TACAGGG 95 3.8926373E-10 44.943394 2 TAGACGC 55 5.002775E-5 43.123047 4 CCACGTC 45 9.689046E-4 42.164753 9 GGTATCA 3580 0.0 40.643982 1 GATACGC 50 0.0016265361 37.94828 9 TGGGTAT 275 0.0 37.94828 6 ATGGGTA 465 0.0 36.72414 5 GTATCAA 5925 0.0 36.59606 1 GACCGGT 65 1.3387136E-4 36.488728 6 >>END_MODULE