FastQCFastQC Report
Thu 26 May 2016
SRR936369_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936369_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019703
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60090.2975189916537233No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG55970.27711995278513724No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG55200.2733075110548432No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42240.20913966063327133No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG38940.192800624646297No Hit
AGTCTGCACCGTCTCTTCGCGGTTAGCTCCTACTCCGGATCAGCCATGAC38140.18883964622521232No Hit
GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC31850.157696453389434No Hit
GAACAGACCCGGGGATTCCCACTGTACTCGCTTCCAGCCGCCTTTACAAG30540.1512103512249078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30480.15091327784332648No Hit
GCTCTGGAGGGCGTAGGCCACTTCTTCCGCGAATTGGCCGAGGAGAAGCG30320.15012108215910952No Hit
GTACAGAGGCCTCCTAGTCGTGCTTGAGAGTGAGGCGCTCAAAGAGATAC29720.14715034834329602No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC29510.1461105915077613No Hit
CCCCTGGGGCGCGCCAGTCTGCGCTGGTTGTGGCCCCGCCACCCTGCGGA29320.14516985913275368No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG26900.13318789940897252No Hit
GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG26630.13185106919185643No Hit
CTCCTACACCTACCTCTCTCTGGGCTTCTTTTTTGATCGGGATGACGTGG26570.13155399581027508No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25290.1252164303365396No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA23320.11546252097461855No Hit
GTTCCAGTAGTGACTGATTCACACTCTTTTCCAAGTGCAGTGCACACTCC22880.11328398284302196No Hit
CTCCAGAGCCACGTCATCCCGATCAAAAAAGAAGCCCAGAGAGAGGTAGG22860.11318495838249486No Hit
GTGTAGGAGGCCCGCAGGTGCAAGTTGACCAGGCGGTTCACGGCAGCTTC22160.10971910226404576No Hit
GTGCAGAGCAGAGGGGGGAGCCCCTTGGAAGGTACAGAGGCCTCCTAGTC20590.10194568211266705No Hit
CTCCGGGACCATCTCCTCGCTGCCTTCAGCTCCTAGGACCAGTCTGCACC20410.10105446196792299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG95200.030.7888371
ATATAGG6700.030.3826562
GAGTGCA23750.028.9025699
TACATGG100900.028.7608282
ACATGGG99700.028.3004883
TATAACG856.5362273E-427.8473032
TATAGGC8250.027.5441953
AGAGTGC25800.027.5234998
CATGGGG51600.026.7902014
GTACGCT909.1261545E-426.3008823
CTTACTA7600.026.1618089
GTCCAAG15150.025.6295411
TACCTGG13650.025.3175852
GTATCAA92200.024.7545911
ACTTGAC11550.024.182558
GTACCTG16200.023.6760711
GAAGAGT29150.023.5484056
GATGTGC23000.022.8468958
ATGTGCA25300.022.641079
TGTACGC1050.002240176822.5430552
Produced by FastQC (version 0.11.5)