FastQCFastQC Report
Thu 26 May 2016
SRR936369_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936369_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019703
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63190.3128677830354265No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG62670.31029314706172145No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG56700.280734345594377No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47280.23409382468610485No Hit
AGTCTGCACCGTCTCTTCGCGGTTAGCTCCTACTCCGGATCAGCCATGAC40310.19958380019240454No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG35820.17735280880406673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32340.16012255267234834No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC31780.15734986777758908No Hit
GCTCTGGAGGGCGTAGGCCACTTCTTCCGCGAATTGGCCGAGGAGAAGCG31440.1556664519486281No Hit
GAACAGACCCGGGGATTCCCACTGTACTCGCTTCCAGCCGCCTTTACAAG31400.15546840302757384No Hit
GTACAGAGGCCTCCTAGTCGTGCTTGAGAGTGAGGCGCTCAAAGAGATAC30880.1528937670538688No Hit
GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC30490.15096279007359004No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG29430.1457144936656528No Hit
CCCCTGGGGCGCGCCAGTCTGCGCTGGTTGTGGCCCCGCCACCCTGCGGA28970.14343693107352912No Hit
CTCCTACACCTACCTCTCTCTGGGCTTCTTTTTTGATCGGGATGACGTGG25700.1272464317773455No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25690.12719691954708193No Hit
CTCCAGAGCCACGTCATCCCGATCAAAAAAGAAGCCCAGAGAGAGGTAGG24810.12283984328388876No Hit
GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG24800.12279033105362522No Hit
GTGTAGGAGGCCCGCAGGTGCAAGTTGACCAGGCGGTTCACGGCAGCTTC23540.11655179004041684No Hit
GTGCAGAGCAGAGGGGGGAGCCCCTTGGAAGGTACAGAGGCCTCCTAGTC23130.11452178859961094No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA23050.11412569075750247No Hit
GTTCCAGTAGTGACTGATTCACACTCTTTTCCAAGTGCAGTGCACACTCC22720.11249178715880502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCT1153.9057395E-628.8755463
GTACATG95050.027.9738621
GAGTGCA25450.027.5869669
TACATGG99000.026.5515942
ACATGGG99250.025.9059053
TACCTGG14300.025.5488132
AGAGTGC27700.025.5174038
GTATCAA95300.025.255171
ACTTGAC12000.024.5098388
GTCCAAG15700.023.9342671
GACTTGA12800.023.7191987
CATGGGG47000.023.7191964
TATAGGG2653.274181E-1123.2762762
CCAAGAC17900.023.056653
CAAGACT16700.023.009044
GTACCTG16150.022.678321
GAAGAGT31000.022.6480086
GATGTGC23150.021.9261688
TATACGA1302.9685104E-421.8946445
AGTACAT72000.021.8787042
Produced by FastQC (version 0.11.5)