##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936369_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2019703 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.44559670406986 34.0 31.0 34.0 31.0 34.0 2 32.5764867408723 34.0 31.0 34.0 31.0 34.0 3 32.655232477250365 34.0 31.0 34.0 31.0 34.0 4 36.156704723417256 37.0 35.0 37.0 35.0 37.0 5 36.11368849776427 37.0 35.0 37.0 35.0 37.0 6 36.15377508475256 37.0 36.0 37.0 35.0 37.0 7 36.098469428425865 37.0 36.0 37.0 35.0 37.0 8 36.078395189787805 37.0 36.0 37.0 35.0 37.0 9 37.83726765767046 39.0 38.0 39.0 35.0 39.0 10-11 37.7654199652127 39.0 38.0 39.0 35.0 39.0 12-13 37.69956647091181 39.0 37.0 39.0 35.0 39.0 14-15 39.04290358532913 40.0 38.0 41.0 35.5 41.0 16-17 38.96421181728205 40.0 38.0 41.0 35.0 41.0 18-19 38.969644546747716 40.0 38.0 41.0 35.0 41.0 20-21 38.92111909523331 40.0 38.0 41.0 35.0 41.0 22-23 38.79665846909174 40.0 38.0 41.0 35.0 41.0 24-25 38.93759874595423 40.0 38.0 41.0 35.0 41.0 26-27 38.8492040661424 40.0 38.0 41.0 35.0 41.0 28-29 38.741863531420215 40.0 38.0 41.0 35.0 41.0 30-31 38.61555288079485 40.0 38.0 41.0 34.0 41.0 32-33 38.468674107034545 40.0 38.0 41.0 34.0 41.0 34-35 38.322178310375335 40.0 38.0 41.0 34.0 41.0 36-37 38.15467571222105 40.0 38.0 41.0 33.5 41.0 38-39 38.0147477129063 40.0 37.0 41.0 33.0 41.0 40-41 37.83650021810138 40.0 37.0 41.0 33.0 41.0 42-43 37.85845443612254 40.0 37.0 41.0 33.0 41.0 44-45 37.72491896085712 40.0 37.0 41.0 33.0 41.0 46-47 37.48370676282602 40.0 36.5 41.0 33.0 41.0 48-49 37.237058121911986 40.0 36.0 41.0 32.0 41.0 50-51 36.99690820878119 39.5 35.5 41.0 31.5 41.0 52-53 36.73909183677006 39.0 35.0 41.0 31.0 41.0 54-55 36.50190102208097 39.0 35.0 41.0 31.0 41.0 56-57 36.230824779682955 38.5 35.0 41.0 30.5 41.0 58-59 35.94863947818071 38.0 35.0 40.0 30.0 41.0 60-61 35.648069047775834 37.5 35.0 40.0 29.5 41.0 62-63 35.328692634511114 37.0 34.5 40.0 29.0 41.0 64-65 34.94517263181765 36.5 34.0 39.5 29.0 41.0 66-67 34.59566480814259 36.0 34.0 39.0 28.0 41.0 68-69 34.25148796629999 35.5 34.0 39.0 28.0 40.5 70-71 33.895435120906384 35.0 34.0 38.0 28.0 40.0 72-73 33.5063392984018 35.0 33.0 37.0 27.0 39.5 74-75 33.095304854228566 35.0 33.0 37.0 26.0 39.0 76-77 32.39627806662663 35.0 32.0 36.0 26.0 39.0 78-79 32.47705826054623 35.0 33.0 36.0 26.0 37.5 80-81 32.34426571629591 35.0 33.0 35.5 26.0 37.0 82-83 32.09904451298037 35.0 33.0 35.0 26.0 37.0 84-85 31.83564836017969 35.0 32.5 35.0 25.5 36.0 86-87 31.424948866244193 35.0 32.0 35.0 24.5 36.0 88-89 30.944273737277214 34.0 31.5 35.0 21.5 36.0 90-91 30.740425448692207 34.0 31.0 35.0 20.0 35.0 92-93 30.464676984685372 34.0 31.0 35.0 19.5 35.0 94-95 30.309985428550632 34.0 31.0 35.0 18.5 35.0 96-97 30.039666475714498 34.0 31.0 35.0 13.5 35.0 98-99 29.577463122053096 34.0 31.0 35.0 2.0 35.0 100-101 26.144236058469986 30.5 24.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11733727148557449 1101 2 0.145394070828317 1101 3 0.20494877134073164 1101 4 0.1823265952893962 1101 5 0.12233709710631047 1101 6 0.1986708132524484 1101 7 0.17895847554672173 1101 8 0.11679959754918201 1101 9 0.2383928701959661 1101 10-11 0.23297072001133046 1101 12-13 0.2908779114420881 1101 14-15 0.3401523084481468 1101 16-17 0.3724093946286686 1101 18-19 0.3869013847850198 1101 20-21 0.3201992639915048 1101 22-23 0.3230346546879659 1101 24-25 0.23178153896665066 1101 26-27 0.34744349192169466 1101 28-29 0.34951305572101177 1101 30-31 0.24145079564019056 1101 32-33 0.36048626233182546 1101 34-35 0.28576532400200705 1101 36-37 0.331122591432802 1101 38-39 0.27549229735188163 1101 40-41 0.4330347055794661 1101 42-43 0.3109626569731816 1101 44-45 0.297405338999404 1101 46-47 0.32009835068807746 1101 48-49 0.35245495592263865 1101 50-51 0.12298747947124156 1101 52-53 0.2291939754629695 1101 54-55 0.12263865072198143 1101 56-57 0.21665824922346388 1101 58-59 0.09187433030683678 1101 60-61 0.29012397159108616 1101 62-63 0.32074930344715114 1101 64-65 0.3422777144577438 1101 66-67 0.35239491782191834 1101 68-69 0.3303027374067682 1101 70-71 0.3607520649549798 1101 72-73 0.37361241358267705 1101 74-75 0.39509119541741455 1101 76-77 0.23749568800918297 1101 78-79 0.22601520416319687 1101 80-81 0.35354218176490804 1101 82-83 0.2493114143998838 1101 84-85 0.47324228164183424 1101 86-87 0.42683768199489336 1101 88-89 0.45647978891306806 1101 90-91 0.5872586510135385 1101 92-93 0.7471596891352235 1101 94-95 0.6612773973712898 1101 96-97 0.5630792570852741 1101 98-99 0.600722008233781 1101 100-101 0.6672704898656043 1102 1 0.053203976933964725 1102 2 0.16582124271509713 1102 3 0.11073925747847113 1102 4 0.09365835091164598 1102 5 0.06889797339701431 1102 6 0.04708032182629296 1102 7 0.010658238890059124 1102 8 0.06291955206879152 1102 9 0.1343231027541023 1102 10-11 0.14592635019233313 1102 12-13 0.24024363192280873 1102 14-15 0.21174359313685898 1102 16-17 0.25313278397515404 1102 18-19 0.3352201639539487 1102 20-21 0.24145655209124328 1102 22-23 0.32397979397165244 1102 24-25 0.2571943660221834 1102 26-27 0.3640522614392623 1102 28-29 0.2665759680522797 1102 30-31 0.2992325360582342 1102 32-33 0.21018102151052886 1102 34-35 0.31344308091991735 1102 36-37 0.1937339787168071 1102 38-39 0.3226638205482004 1102 40-41 0.19270903824733665 1102 42-43 0.2933115735087455 1102 44-45 0.35481474180490835 1102 46-47 0.4197018846727687 1102 48-49 0.462016083413161 1102 50-51 0.32840100063151567 1102 52-53 0.13776269844246514 1102 54-55 0.10840305835316855 1102 56-57 0.26865776665540153 1102 58-59 0.26071910865606185 1102 60-61 0.18280546345353343 1102 62-63 0.2497489993631703 1102 64-65 0.26780209406032185 1102 66-67 0.30314190672947205 1102 68-69 0.28448736265631425 1102 70-71 0.2618809767309642 1102 72-73 0.20347147726843673 1102 74-75 0.28754330219056357 1102 76-77 0.051017344076981885 1102 78-79 0.020837513350492998 1102 80-81 0.17866993008914278 1102 82-83 0.15788773947887336 1102 84-85 0.11686759068370733 1102 86-87 0.06698949261667764 1102 88-89 0.15798764882289973 1102 90-91 0.19572177091517418 1102 92-93 0.12056589244841476 1102 94-95 0.04574465545926287 1102 96-97 -0.025616916744990448 1102 98-99 0.29525477658789256 1102 100-101 0.58652462474668 1103 1 0.011943572728284835 1103 2 0.017007539605714328 1103 3 0.06594113972107607 1103 4 -0.0699371081087321 1103 5 0.037227779311230336 1103 6 0.03650405204994911 1103 7 0.07866326593500617 1103 8 0.11131179267113112 1103 9 0.09385747082505702 1103 10-11 0.08447126144380945 1103 12-13 0.08921751851681137 1103 14-15 0.12271302623291547 1103 16-17 0.13858662352569695 1103 18-19 0.10825122751177929 1103 20-21 0.1632089839935631 1103 22-23 0.14141048617356233 1103 24-25 0.05834782577116471 1103 26-27 0.01983881497640283 1103 28-29 0.2574817815499202 1103 30-31 0.10199003660341788 1103 32-33 0.027067631898155753 1103 34-35 0.11414605416236867 1103 36-37 0.06167162974559659 1103 38-39 0.13628420680374376 1103 40-41 0.10107923195496227 1103 42-43 0.2763664109050197 1103 44-45 0.31491036658667326 1103 46-47 0.2509645137365979 1103 48-49 0.059243487867163935 1103 50-51 0.12277541841828565 1103 52-53 0.19882670376699707 1103 54-55 0.18962093814528203 1103 56-57 0.1497803809204612 1103 58-59 0.19823809558297256 1103 60-61 0.0811374816204733 1103 62-63 -0.001351454558317755 1103 64-65 -0.11397647519832077 1103 66-67 -0.08318879795181289 1103 68-69 -0.05192474158049265 1103 70-71 -0.1909321767817289 1103 72-73 -0.12650618591198537 1103 74-75 -0.04237464277161962 1103 76-77 0.054976866785032996 1103 78-79 -0.062186724367713 1103 80-81 0.20590837059593525 1103 82-83 0.1943566626853439 1103 84-85 0.18415536883825112 1103 86-87 0.0338331184925984 1103 88-89 0.15939063303207845 1103 90-91 0.37697916872611614 1103 92-93 0.36158499723608273 1103 94-95 0.2647773715887922 1103 96-97 0.12117818453332418 1103 98-99 -0.05544258723881157 1103 100-101 0.5550457180407165 1104 1 0.040695308797097596 1104 2 0.03301226658604861 1104 3 0.0038434812819545527 1104 4 0.08682442304304061 1104 5 0.0967109790383347 1104 6 0.01484765966185364 1104 7 -0.03141080633419335 1104 8 0.06652487227445647 1104 9 -0.05908349065249041 1104 10-11 -0.024089080653119765 1104 12-13 0.18498090147531343 1104 14-15 0.13274750395031987 1104 16-17 0.11076870973217723 1104 18-19 0.14156851997540798 1104 20-21 0.07172303092224297 1104 22-23 0.032787401940716165 1104 24-25 0.07805677535816358 1104 26-27 0.15414231635308084 1104 28-29 0.1525190613432983 1104 30-31 0.022280721528254332 1104 32-33 0.05340410030779452 1104 34-35 0.08538518088930402 1104 36-37 -0.031664911761680514 1104 38-39 0.059733927814441756 1104 40-41 0.15838290389277887 1104 42-43 0.13161177529672585 1104 44-45 0.21171660912462187 1104 46-47 0.22044016004151246 1104 48-49 0.26353980130981824 1104 50-51 0.18528303275516578 1104 52-53 0.11017084742821481 1104 54-55 0.17947803227066572 1104 56-57 0.16068776621808922 1104 58-59 0.15052178750080714 1104 60-61 -0.08345767931614745 1104 62-63 -0.06194939320503323 1104 64-65 -0.04103112786273044 1104 66-67 -0.05191763009527506 1104 68-69 0.09440636633597421 1104 70-71 0.05232822135671711 1104 72-73 0.030314432493995014 1104 74-75 0.057958560610366305 1104 76-77 0.10862078384358398 1104 78-79 0.22151737640955815 1104 80-81 0.2251464495901132 1104 82-83 0.1702831751972269 1104 84-85 0.26521455355596757 1104 86-87 0.2583001149108135 1104 88-89 0.30205223971807627 1104 90-91 0.5343516742693559 1104 92-93 0.5926746393013396 1104 94-95 0.4743555982401908 1104 96-97 0.4332587868578308 1104 98-99 0.25486844319416946 1104 100-101 0.24538830228574326 1105 1 0.0524104006092756 1105 2 0.029495071806721285 1105 3 0.048381575334609295 1105 4 0.09872859222875974 1105 5 0.0029428270177547233 1105 6 0.0803709713016687 1105 7 0.14316095415990304 1105 8 0.07464749398931048 1105 9 0.1278506108447175 1105 10-11 0.154859909946353 1105 12-13 0.14785653716648284 1105 14-15 0.10458119884226846 1105 16-17 0.16169779682631003 1105 18-19 0.116978088831587 1105 20-21 0.16036979408357155 1105 22-23 0.046726671947951104 1105 24-25 0.11499947128144328 1105 26-27 0.18918949678860741 1105 28-29 0.044880394724053474 1105 30-31 0.098903882617428 1105 32-33 0.1906200397082003 1105 34-35 0.11332891999808936 1105 36-37 0.06697687255972085 1105 38-39 0.09050226950511586 1105 40-41 0.06327698500044932 1105 42-43 0.19506679384883086 1105 44-45 0.12364715430760498 1105 46-47 0.11626534348805961 1105 48-49 0.12248393161316784 1105 50-51 -0.02746753921870493 1105 52-53 0.13596657273850354 1105 54-55 0.2374228382737229 1105 56-57 0.20056827405976208 1105 58-59 0.255978655336321 1105 60-61 0.2646542141840911 1105 62-63 0.3162586985952913 1105 64-65 0.23877523082712315 1105 66-67 0.2189173332154155 1105 68-69 0.1473447709734117 1105 70-71 0.2207013261375863 1105 72-73 0.21483942745588536 1105 74-75 0.13485437245763165 1105 76-77 0.14416229195790464 1105 78-79 0.05273618457010798 1105 80-81 0.1076298238210569 1105 82-83 0.2873081405231588 1105 84-85 0.36101573188142666 1105 86-87 0.3385963558866685 1105 88-89 0.4272306481489103 1105 90-91 0.5327874887245869 1105 92-93 0.536044187767974 1105 94-95 0.3381194234117828 1105 96-97 0.31101552310971314 1105 98-99 0.3607286563359331 1105 100-101 0.7277525399897833 1106 1 0.018682364114660288 1106 2 0.05947319972071341 1106 3 0.02404859396587966 1106 4 -0.016384973492627353 1106 5 -0.018592150035985355 1106 6 0.045017384005305416 1106 7 0.08475025717856965 1106 8 0.06764705517630176 1106 9 -0.011863337923863071 1106 10-11 0.09007556825729068 1106 12-13 0.07525783852131696 1106 14-15 0.14681495133343958 1106 16-17 0.09876926849544532 1106 18-19 0.11498943169081599 1106 20-21 0.11870512721578308 1106 22-23 0.17933762078966708 1106 24-25 0.18477473959731583 1106 26-27 0.08181972369150259 1106 28-29 0.20038761228388324 1106 30-31 0.21566985358263935 1106 32-33 0.020987099935290132 1106 34-35 0.25017087189321785 1106 36-37 0.15067816888648622 1106 38-39 0.1437323446518448 1106 40-41 0.09580199962755387 1106 42-43 0.11587618673274136 1106 44-45 0.07767977731236897 1106 46-47 0.2780247893952108 1106 48-49 0.14699575213865046 1106 50-51 0.18984917635732756 1106 52-53 0.20929719777278422 1106 54-55 0.14100310677243755 1106 56-57 0.36586622123764556 1106 58-59 0.3674360173387683 1106 60-61 0.26760106067267486 1106 62-63 0.15965844810619956 1106 64-65 0.2799550893099294 1106 66-67 0.23264595250856246 1106 68-69 0.323906156799751 1106 70-71 0.2595991284157648 1106 72-73 0.2097205137009297 1106 74-75 0.42931961481040304 1106 76-77 0.287798703381668 1106 78-79 0.07938778989437623 1106 80-81 0.1884665510987702 1106 82-83 0.16388941006405133 1106 84-85 0.29049568132650805 1106 86-87 0.29338404581042354 1106 88-89 0.32968017322524545 1106 90-91 0.5288961657947802 1106 92-93 0.5481906804635663 1106 94-95 0.5055188735242275 1106 96-97 0.4524441565728594 1106 98-99 0.2543544973942069 1106 100-101 0.4069913708273667 1107 1 -0.07723464543575176 1107 2 0.0041655008795942194 1107 3 -0.00365303111433235 1107 4 -0.022694899505232513 1107 5 -0.07392462144136402 1107 6 -0.05497294289817489 1107 7 0.05564957204021681 1107 8 -0.028022228731444443 1107 9 -0.04663458098754347 1107 10-11 -0.02547310865184471 1107 12-13 0.025244980380819015 1107 14-15 0.06348063496184864 1107 16-17 0.0734776600246434 1107 18-19 0.04167620738822109 1107 20-21 0.07969128760987587 1107 22-23 -0.010122360902229843 1107 24-25 0.07105097492252099 1107 26-27 0.08514160659513692 1107 28-29 0.1312379482470405 1107 30-31 -0.0978788516380007 1107 32-33 -0.04348703750761729 1107 34-35 -0.06796842541530168 1107 36-37 -0.10470573508089842 1107 38-39 0.05229798958786347 1107 40-41 0.0695389527437058 1107 42-43 -0.07888040512864336 1107 44-45 -0.009612840935332656 1107 46-47 0.09967117401640024 1107 48-49 -0.028540278966261212 1107 50-51 0.13884895458389224 1107 52-53 0.18737170892751465 1107 54-55 0.22942051740245972 1107 56-57 0.10221594850350613 1107 58-59 0.09838945435511448 1107 60-61 0.24304260213958884 1107 62-63 0.17996487292972319 1107 64-65 0.16365143901359858 1107 66-67 0.012046857532915567 1107 68-69 0.1744609442747418 1107 70-71 0.3027387796180534 1107 72-73 0.23348448070858296 1107 74-75 0.13382067177314383 1107 76-77 0.008308735090551522 1107 78-79 -0.04864036117580639 1107 80-81 0.2749469831322955 1107 82-83 0.33656538964202554 1107 84-85 0.1453113498554366 1107 86-87 0.29148068712256503 1107 88-89 0.3881923802012004 1107 90-91 0.17878374553884058 1107 92-93 0.49143378661282 1107 94-95 0.40970154405384207 1107 96-97 0.1765827791239154 1107 98-99 0.2863541623747672 1107 100-101 0.4803175712350516 1108 1 -0.10204336936252645 1108 2 -0.08558104974854075 1108 3 0.022738036418807894 1108 4 -0.018215403660029494 1108 5 -0.03241554125862223 1108 6 -0.06602762521753647 1108 7 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-0.5625713920108204 2316 90-91 -0.7021980333329871 2316 92-93 -0.724848334800587 2316 94-95 -0.6639254676724526 2316 96-97 -0.6563589540757029 2316 98-99 -0.9373021542395854 2316 100-101 -0.8526227509884698 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 21.0 5 38.0 6 83.0 7 218.0 8 412.0 9 718.0 10 1104.0 11 1854.0 12 2634.0 13 3923.0 14 4624.0 15 5934.0 16 7993.0 17 10512.0 18 10239.0 19 7126.0 20 7024.0 21 7475.0 22 8053.0 23 8846.0 24 10523.0 25 12815.0 26 16014.0 27 20051.0 28 25414.0 29 31468.0 30 39021.0 31 48221.0 32 60386.0 33 77946.0 34 110767.0 35 173952.0 36 300070.0 37 483243.0 38 455550.0 39 65412.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.28032516547678 17.64344382327566 12.587454243820495 24.488776767427062 2 17.006803731727256 19.111607644702485 37.60850736357005 26.273081260000207 3 19.649077116784003 22.88489941342861 28.33515620861087 29.13086726117652 4 13.204565225679222 14.727462404125754 34.51859010953591 37.549382260659115 5 14.999235036042426 36.48714687258473 32.664060012784056 15.849558078588782 6 35.65380652501878 33.90280650174803 15.812621954812167 14.630765018421025 7 31.90607727967924 31.058774483178965 18.779493816665124 18.255654420476674 8 28.82394094577272 33.676634633904094 18.185644126884 19.313780293439184 9 28.036894533503194 15.167972716780637 18.67715203671035 38.11798071300582 10-11 27.337149754938 24.637952401676888 25.74673477602784 22.27816306735727 12-13 26.936343347596985 21.58535724939688 27.06334224966301 24.414957153343128 14-15 23.55319074141099 23.59329564792447 24.53130485026759 28.32220876039695 16-17 24.61557885668239 27.175250687914126 24.76882000601577 23.44035044938772 18-19 23.873188285604368 25.373408862590193 26.799979997058976 23.953422854746464 20-21 24.425602285485116 24.984001356635446 26.606245226710367 23.98415113116907 22-23 24.07166997903651 24.497871468167087 27.043469779205054 24.38698877359135 24-25 24.064774685873324 25.050664794392286 27.000139133224764 23.884421386509622 26-27 24.252610543478774 25.600866290214647 26.766738634706616 23.379784531599963 28-29 23.502377390945266 25.01934740210066 27.521190542326373 23.9570846646277 30-31 23.63346893264068 25.04906727613736 27.44814019674443 23.869323594477528 32-33 23.729965786489874 25.527214842028652 27.226898453707783 23.515920917773695 34-35 24.25313034897406 25.223539768763402 26.61505904697962 23.908270835282917 36-37 24.725658717389674 25.208056735116553 26.247987635796676 23.8182969116971 38-39 23.96644948099978 25.149818005194334 26.79538377100961 24.08834874279627 40-41 23.841595061010768 24.95159516251177 27.469810259968824 23.736999516508643 42-43 24.367448604773625 25.289875290803682 26.392335978304814 23.95034012611788 44-45 23.841049273447755 25.474584906865545 26.366961493003345 24.31740432668336 46-47 23.750337659585163 25.71075640451408 26.12208829733158 24.41681763856918 48-49 24.55346299386229 25.35431908909645 26.156363655271132 23.935854261770135 50-51 24.362998150338306 25.24777073801031 25.802297878471954 24.58693323317943 52-53 24.315422739239953 25.476731973908795 26.011534361195743 24.196310925655514 54-55 24.1178998631973 25.080222429404014 25.90183081917733 24.900046888221354 56-57 24.083604112124082 25.90093519257788 25.857833398320246 24.1576272969778 58-59 24.379477221008603 25.18443625387935 26.085772513106114 24.35031401200593 60-61 24.49377818532551 25.382983737921283 26.21111489611164 23.91212318064157 62-63 24.208485108949187 25.444087571291423 26.26465376344938 24.08277355631001 64-65 23.9046327947683 25.13217388032211 26.35756116786355 24.60563215704604 66-67 24.17167276574823 25.59311938438473 25.928688524996 24.306519324871033 68-69 23.308699348369537 25.549919963479777 26.735094219298578 24.406286468852105 70-71 23.867523922482565 25.41691206196166 26.498409046291766 24.21715496926401 72-73 23.824751359003088 25.640906302437173 25.89914040795592 24.635201930603824 74-75 23.671524971740894 25.823475035685938 25.75695535432685 24.748044638246316 76-77 24.10583431529703 25.644741531694713 25.73832054320967 24.51110360979859 78-79 23.83359700563426 25.448589848814663 26.236905597386272 24.4809075481648 80-81 23.76686376909185 26.912638280121453 25.699499053685553 23.620998897101146 82-83 24.227398780909866 26.10190706257306 25.464956976347512 24.20573718016956 84-85 24.248275725482625 25.44945016314385 25.14098698315088 25.161287128222646 86-87 24.076478777830683 25.274883251609666 26.04543130291972 24.60320666763993 88-89 24.103464770964642 25.608511933176302 25.820349064363178 24.467674231495877 90-91 24.104692362630757 25.808093801559718 26.057542258480748 24.029671577328774 92-93 23.979845701879665 25.29248710375198 25.83972103980407 24.88794615456429 94-95 24.066885896233924 25.328120214978 25.947955795278236 24.657038093509836 96-97 24.00661304829293 25.441742564962578 25.666163360018736 24.885481026725756 98-99 23.942027320697093 25.32851135424563 25.581996998476463 25.147464326580817 100-101 23.816477958025285 25.44236943058645 25.5053988429919 25.235753768396368 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 565.0 1 375.0 2 486.0 3 824.5 4 654.5 5 582.0 6 1183.5 7 2234.5 8 3169.0 9 3216.0 10 2362.5 11 1747.0 12 1714.5 13 1764.0 14 1850.0 15 1943.5 16 1946.5 17 1917.0 18 1941.0 19 1963.5 20 2010.5 21 2230.5 22 2553.0 23 3064.0 24 3740.0 25 4391.0 26 5183.0 27 6244.0 28 7328.0 29 8919.0 30 12184.5 31 15629.5 32 19824.5 33 24051.5 34 27486.0 35 31180.5 36 35922.0 37 39187.0 38 39811.5 39 40908.0 40 41889.5 41 46556.0 42 51613.5 43 56130.5 44 58906.0 45 60094.0 46 60266.0 47 59139.0 48 70116.0 49 73878.5 50 69021.0 51 69665.5 52 71362.5 53 79085.0 54 88385.0 55 88605.5 56 80456.0 57 73990.0 58 69547.5 59 63157.0 60 56032.0 61 49377.0 62 43973.0 63 37171.5 64 31942.5 65 27118.5 66 19716.0 67 17895.0 68 13063.0 69 6170.5 70 4734.0 71 3952.0 72 3276.5 73 2691.5 74 2283.0 75 2043.0 76 1262.5 77 560.0 78 263.5 79 139.0 80 76.5 81 41.0 82 18.5 83 10.0 84 8.5 85 7.0 86 5.5 87 2.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2585033542060392 2 0.018319525197516666 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.951223026355855E-4 12-13 2.4756115131779275E-5 14-15 0.0 16-17 5.198784177673648E-4 18-19 0.0 20-21 2.4756115131779275E-5 22-23 4.951223026355855E-5 24-25 0.002772684894759279 26-27 0.0027974410098910584 28-29 0.002574635973705045 30-31 0.001336830217116081 32-33 0.0016339035986974323 34-35 0.001980489210542342 36-37 7.426834539533782E-5 38-39 1.2378057565889638E-4 40-41 9.159762598758332E-4 42-43 0.004183783457270698 44-45 0.0047531741053016205 46-47 0.014234766200773083 48-49 0.014927937424462905 50-51 0.0071545172730842115 52-53 0.008516103605332071 54-55 2.970733815813513E-4 56-57 0.003243051082263085 58-59 0.0017329280592245492 60-61 0.0017329280592245492 62-63 0.0 64-65 7.426834539533783E-4 66-67 0.0 68-69 0.0 70-71 1.2378057565889638E-4 72-73 0.0011882935263254051 74-75 0.0 76-77 0.1861412296758484 78-79 0.10135153534950436 80-81 0.001262561871720743 82-83 0.0 84-85 6.436589934262613E-4 86-87 0.12068606126742398 88-89 0.5838729753830142 90-91 0.3099960736801401 92-93 0.29405313553527423 94-95 0.002698416549363941 96-97 0.0029459777006817342 98-99 0.0030697582763406306 100-101 5.446345328991441E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2019703.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.568368620883405 #Duplication Level Percentage of deduplicated Percentage of total 1 61.76170073460449 18.26192733973471 2 15.70363651523462 9.286618263416443 3 7.190654178524644 6.378477401277367 4 4.067440508878052 4.810703212400793 5 2.6569905780468193 3.928143841695122 6 1.713810500396061 3.040474837323083 7 1.2319476791425856 2.5498678168970836 8 0.8934586147485556 2.1134490934711323 9 0.6715244330004749 1.7870293775598987 >10 3.5972066567395933 19.004074413681725 >50 0.2669477440586105 5.5500823958999685 >100 0.21340727718087335 12.806674760684988 >500 0.021574442504615852 4.501546890683552 >1k 0.009198405719022262 5.078148414855475 >5k 5.017312210375778E-4 0.9027819404187513 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6319 0.3128677830354265 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 6267 0.31029314706172145 No Hit CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG 5670 0.280734345594377 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4728 0.23409382468610485 No Hit AGTCTGCACCGTCTCTTCGCGGTTAGCTCCTACTCCGGATCAGCCATGAC 4031 0.19958380019240454 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 3582 0.17735280880406673 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3234 0.16012255267234834 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 3178 0.15734986777758908 No Hit GCTCTGGAGGGCGTAGGCCACTTCTTCCGCGAATTGGCCGAGGAGAAGCG 3144 0.1556664519486281 No Hit GAACAGACCCGGGGATTCCCACTGTACTCGCTTCCAGCCGCCTTTACAAG 3140 0.15546840302757384 No Hit GTACAGAGGCCTCCTAGTCGTGCTTGAGAGTGAGGCGCTCAAAGAGATAC 3088 0.1528937670538688 No Hit GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC 3049 0.15096279007359004 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2943 0.1457144936656528 No Hit CCCCTGGGGCGCGCCAGTCTGCGCTGGTTGTGGCCCCGCCACCCTGCGGA 2897 0.14343693107352912 No Hit CTCCTACACCTACCTCTCTCTGGGCTTCTTTTTTGATCGGGATGACGTGG 2570 0.1272464317773455 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2569 0.12719691954708193 No Hit CTCCAGAGCCACGTCATCCCGATCAAAAAAGAAGCCCAGAGAGAGGTAGG 2481 0.12283984328388876 No Hit GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG 2480 0.12279033105362522 No Hit GTGTAGGAGGCCCGCAGGTGCAAGTTGACCAGGCGGTTCACGGCAGCTTC 2354 0.11655179004041684 No Hit GTGCAGAGCAGAGGGGGGAGCCCCTTGGAAGGTACAGAGGCCTCCTAGTC 2313 0.11452178859961094 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2305 0.11412569075750247 No Hit GTTCCAGTAGTGACTGATTCACACTCTTTTCCAAGTGCAGTGCACACTCC 2272 0.11249178715880502 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.9512230263558556E-5 0.0 12-13 0.0 0.0 0.0 1.9804892105423422E-4 0.0 14-15 0.0 0.0 0.0 2.970733815813513E-4 0.0 16-17 0.0 0.0 0.0 4.7036618750380627E-4 0.0 18-19 0.0 0.0 0.0 6.684151085580405E-4 0.0 20-21 0.0 0.0 0.0 0.001064512950666509 0.0 22-23 0.0 0.0 0.0 0.002153782016464797 0.0 24-25 0.0 0.0 0.0 0.0032430510822630855 0.0 26-27 0.0 0.0 0.0 0.003837197845425788 0.0 28-29 0.0 0.0 0.0 0.004654149644774504 0.0 30-31 0.0 0.0 0.0 0.006882200006634639 0.0 32-33 0.0 0.0 0.0 0.010521348931006192 0.0 34-35 0.0 0.0 0.0 0.015868669799470516 0.0 36-37 0.0 0.0 0.0 0.02574635973705045 0.0 38-39 0.0 0.0 0.0 0.04074856550690869 0.0 40-41 0.0 0.0 0.0 0.057483699335991484 0.0 42-43 0.0 0.0 0.0 0.07570420007298104 0.0 44-45 0.0 0.0 0.0 0.0940237252704977 0.0 46-47 0.0 0.0 0.0 0.11902740155359476 0.0 48-49 0.0 0.0 0.0 0.14682851884658288 0.0 50-51 0.0 0.0 0.0 0.1789619562876324 0.0 52-53 0.0 0.0 0.0 0.21136771099513146 0.0 54-55 0.0 0.0 0.0 0.24459041750197924 0.0 56-57 0.0 0.0 0.0 0.2835070304891363 0.0 58-59 0.0 0.0 0.0 0.3284393794533157 0.0 60-61 0.0 0.0 0.0 0.38025392842412964 0.0 62-63 0.0 0.0 0.0 0.43157335509230815 0.0 64-65 0.0 0.0 0.0 0.4847742465105018 0.0 66-67 0.0 0.0 0.0 0.5461446559221825 0.0 68-69 0.0 0.0 0.0 0.6174917797319704 0.0 70-71 0.0 0.0 0.0 0.700994156071462 0.0 72-73 0.0 0.0 0.0 0.7829121410425196 0.0 74-75 0.0 0.0 0.0 0.8665135418425383 0.0 76-77 0.0 0.0 0.0 0.9668005642413762 0.0 78-79 0.0 0.0 0.0 1.0770395449231893 0.0 80-81 0.0 0.0 0.0 1.208742077424255 0.0 82-83 0.0 0.0 0.0 1.3391325358233364 0.0 84-85 0.0 0.0 0.0 1.474994095666541 0.0 86-87 0.0 0.0 0.0 1.6271204231513248 0.0 88-89 0.0 0.0 0.0 1.787465780859859 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGCT 115 3.9057395E-6 28.875546 3 GTACATG 9505 0.0 27.973862 1 GAGTGCA 2545 0.0 27.586966 9 TACATGG 9900 0.0 26.551594 2 ACATGGG 9925 0.0 25.905905 3 TACCTGG 1430 0.0 25.548813 2 AGAGTGC 2770 0.0 25.517403 8 GTATCAA 9530 0.0 25.25517 1 ACTTGAC 1200 0.0 24.509838 8 GTCCAAG 1570 0.0 23.934267 1 GACTTGA 1280 0.0 23.719198 7 CATGGGG 4700 0.0 23.719196 4 TATAGGG 265 3.274181E-11 23.276276 2 CCAAGAC 1790 0.0 23.05665 3 CAAGACT 1670 0.0 23.00904 4 GTACCTG 1615 0.0 22.67832 1 GAAGAGT 3100 0.0 22.648008 6 GATGTGC 2315 0.0 21.926168 8 TATACGA 130 2.9685104E-4 21.894644 5 AGTACAT 7200 0.0 21.878704 2 >>END_MODULE