FastQCFastQC Report
Thu 26 May 2016
SRR936368_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936368_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2923148
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39430.1348888253348787No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33220.1136446050627611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG92250.026.7812252
GTACATG95600.026.6430821
GTATCAA94000.024.7294061
ACATGGG94900.024.6377983
TCAACGC102600.022.1425484
CAACGCA102700.022.0288185
ATCAACG103900.021.820323
CATGGGG59800.021.765334
AACGCAG105300.021.528746
GTACCTG25450.021.2068961
GAGTACA70000.021.1692941
GTGTAGC22700.021.0647141
TAGATCG1353.8911088E-421.0354215
TATCAAC109450.020.7563882
GGTATCA70600.020.721151
TAGCCCT19950.020.6400184
TACCTGG25350.020.537192
AGTACAT66950.019.86472
GTATAGG7550.019.439041
GTGTAGG14150.019.071221
Produced by FastQC (version 0.11.5)