##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936368_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2923148 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.638521210694773 33.0 31.0 34.0 30.0 34.0 2 31.731804547699944 33.0 31.0 34.0 30.0 34.0 3 31.805791222339753 34.0 31.0 34.0 30.0 34.0 4 35.29122644491486 37.0 35.0 37.0 33.0 37.0 5 35.267964536862316 37.0 35.0 37.0 33.0 37.0 6 35.3535096409761 37.0 35.0 37.0 33.0 37.0 7 35.29355817769063 37.0 35.0 37.0 33.0 37.0 8 35.29316989765828 37.0 35.0 37.0 33.0 37.0 9 36.95544358342445 39.0 37.0 39.0 33.0 39.0 10-11 36.87378196382804 39.0 37.0 39.0 33.0 39.0 12-13 36.77869971003863 39.0 37.0 39.0 33.0 39.0 14-15 38.01560475213708 40.0 38.0 41.0 33.0 41.0 16-17 37.957526269624395 40.0 38.0 41.0 33.0 41.0 18-19 37.89084148322288 40.0 38.0 41.0 33.0 41.0 20-21 37.79884391758474 40.0 38.0 41.0 32.5 41.0 22-23 37.63775217676286 40.0 38.0 41.0 32.0 41.0 24-25 37.79924742093114 40.0 38.0 41.0 32.5 41.0 26-27 37.74915946780662 40.0 38.0 41.0 32.0 41.0 28-29 37.61979174506388 40.0 38.0 41.0 32.0 41.0 30-31 37.47937942245825 40.0 37.5 41.0 32.0 41.0 32-33 37.30589368037472 40.0 37.0 41.0 31.0 41.0 34-35 37.04954299269144 40.0 37.0 41.0 30.5 41.0 36-37 36.85670961579777 40.0 37.0 41.0 30.0 41.0 38-39 36.69457174251868 40.0 36.5 41.0 30.0 41.0 40-41 36.51885535730658 40.0 36.0 41.0 30.0 41.0 42-43 36.335468816495094 40.0 36.0 41.0 30.0 41.0 44-45 36.16795574497083 40.0 36.0 41.0 29.0 41.0 46-47 35.92459584666941 39.0 35.0 41.0 28.5 41.0 48-49 35.77396611461342 39.0 35.0 41.0 28.0 41.0 50-51 35.47465933986237 38.5 35.0 40.5 27.5 41.0 52-53 35.49699844140632 39.0 35.0 40.5 28.0 41.0 54-55 35.58234530034059 39.0 35.0 41.0 28.0 41.0 56-57 35.415506501894534 39.0 35.0 41.0 27.0 41.0 58-59 35.17727959720138 39.0 35.0 41.0 26.5 41.0 60-61 34.87464353498352 38.0 34.5 40.0 26.0 41.0 62-63 34.56592669957183 37.5 34.0 40.0 26.0 41.0 64-65 34.234877775603564 37.0 34.0 40.0 26.0 41.0 66-67 33.87015676250398 36.5 34.0 39.5 25.0 41.0 68-69 33.48276652430873 36.0 33.5 39.0 24.5 41.0 70-71 33.07167905969865 35.5 33.0 39.0 23.5 40.5 72-73 32.66351874759677 35.0 33.0 38.0 22.0 40.0 74-75 32.250213639542025 35.0 32.5 37.0 21.5 39.5 76-77 31.857119276889165 35.0 32.0 37.0 20.0 39.0 78-79 31.46409435991609 35.0 32.0 36.0 19.0 39.0 80-81 31.073127327114467 35.0 31.5 36.0 17.5 37.5 82-83 30.72809929569081 35.0 31.0 35.0 14.5 37.0 84-85 30.4111926594206 35.0 31.0 35.0 9.5 36.5 86-87 30.09880221596717 34.0 31.0 35.0 7.0 36.0 88-89 29.77927477500284 34.0 30.5 35.0 2.0 36.0 90-91 29.424221934708747 34.0 30.0 35.0 2.0 35.5 92-93 29.11908411753356 34.0 30.0 35.0 2.0 35.0 94-95 28.73911054110158 34.0 29.5 35.0 2.0 35.0 96-97 28.31529826748423 34.0 29.0 35.0 2.0 35.0 98-99 27.8263113944282 34.0 29.0 35.0 2.0 35.0 100-101 24.58496473664693 30.0 22.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27741117553081907 1101 2 0.23667346377312626 1101 3 0.3382819356191433 1101 4 0.09223998906895758 1101 5 0.29365418148269384 1101 6 0.22574241009275653 1101 7 0.36118898924302556 1101 8 0.2698609795341085 1101 9 0.3377035012552483 1101 10-11 0.5342762919390225 1101 12-13 0.4456808146238487 1101 14-15 0.4990107545394338 1101 16-17 0.5573548620979523 1101 18-19 0.5422200800706065 1101 20-21 0.5752484186059448 1101 22-23 0.5579502444613524 1101 24-25 0.5197139015288954 1101 26-27 0.46775148078425133 1101 28-29 0.546764449624142 1101 30-31 0.6657212180944114 1101 32-33 0.6323591903874615 1101 34-35 0.41439289650812583 1101 36-37 0.4520055395682334 1101 38-39 0.49991785984414605 1101 40-41 0.358521339378818 1101 42-43 0.36236298492215013 1101 44-45 0.3150736656120543 1101 46-47 0.33972922928028026 1101 48-49 0.34285855991240055 1101 50-51 0.5332071972822305 1101 52-53 0.6477811478730331 1101 54-55 0.6218530376094691 1101 56-57 0.518389607587622 1101 58-59 0.5707246090332561 1101 60-61 0.45741128358852023 1101 62-63 0.41634116032835067 1101 64-65 0.505337891413383 1101 66-67 0.6071156229188261 1101 68-69 0.6322733492970372 1101 70-71 0.706474807250359 1101 72-73 0.6570923559557187 1101 74-75 0.5579880381047104 1101 76-77 0.5063348966508272 1101 78-79 0.6019611522047441 1101 80-81 0.6403005948265523 1101 82-83 0.6677400453021853 1101 84-85 0.6194536331234133 1101 86-87 0.6796126898676462 1101 88-89 0.5731033178963578 1101 90-91 0.40374010895484247 1101 92-93 0.47873779305849595 1101 94-95 0.6575576604568241 1101 96-97 0.8166016853926408 1101 98-99 0.692193758941297 1101 100-101 0.9600234120161524 1102 1 0.4420621425218805 1102 2 0.38909465585890146 1102 3 0.3765183222174642 1102 4 0.41591145973730903 1102 5 0.41258912935679604 1102 6 0.4256099286030235 1102 7 0.3347372645073676 1102 8 0.3579778851627182 1102 9 0.4777946287098729 1102 10-11 0.5796267488144338 1102 12-13 0.6525543752805589 1102 14-15 0.6859461605271022 1102 16-17 0.5980173933611042 1102 18-19 0.6734108038900288 1102 20-21 0.6159440702415324 1102 22-23 0.540847441301608 1102 24-25 0.7063465811217995 1102 26-27 0.6804242019788163 1102 28-29 0.5915308117072016 1102 30-31 0.6051109365402922 1102 32-33 0.3841585242655796 1102 34-35 0.43097382164989284 1102 36-37 0.43896385123402837 1102 38-39 0.45875078458971075 1102 40-41 0.4517047333812556 1102 42-43 0.39998153728330266 1102 44-45 0.3885875823592002 1102 46-47 0.536452479162655 1102 48-49 0.5839566085962531 1102 50-51 0.492496997445663 1102 52-53 0.4250546911957187 1102 54-55 0.5307682989818332 1102 56-57 0.5584921878813063 1102 58-59 0.5590798575289071 1102 60-61 0.5436328991198067 1102 62-63 0.5648036940677983 1102 64-65 0.49231694200397413 1102 66-67 0.4758373003573553 1102 68-69 0.5547534151663385 1102 70-71 0.687453189242774 1102 72-73 0.48746373747764693 1102 74-75 0.615309188093569 1102 76-77 0.6201303056100471 1102 78-79 0.5613927199436404 1102 80-81 0.5423097942421471 1102 82-83 0.6749525480880081 1102 84-85 0.7330450577327774 1102 86-87 0.6401808744997908 1102 88-89 0.5810648982643336 1102 90-91 0.6031216556452854 1102 92-93 0.6998862342342349 1102 94-95 0.6776790605509255 1102 96-97 0.7009908466733776 1102 98-99 0.8319963986659324 1102 100-101 0.8476122489029585 1103 1 0.07510237322201974 1103 2 0.07662656622622777 1103 3 0.16831440315160506 1103 4 0.16368659801556618 1103 5 0.17242402275253 1103 6 0.06877992813027589 1103 7 0.1898506054046436 1103 8 0.0527794499525811 1103 9 0.09172586777761893 1103 10-11 0.1616301880429205 1103 12-13 0.21478254622557813 1103 14-15 0.2609966852753658 1103 16-17 0.31058357782666235 1103 18-19 0.18687195597247808 1103 20-21 0.2864677548252814 1103 22-23 0.2971187148798222 1103 24-25 0.3045894426544393 1103 26-27 0.36664217342494965 1103 28-29 0.3342103370700329 1103 30-31 0.27735541722861257 1103 32-33 0.21940825243652284 1103 34-35 0.0917828676480994 1103 36-37 0.08748533754803134 1103 38-39 0.11804556547185996 1103 40-41 0.1616779050353898 1103 42-43 -0.04701652445735505 1103 44-45 0.07980129908968792 1103 46-47 0.05654921485026421 1103 48-49 0.15290545745929762 1103 50-51 0.1849658552908906 1103 52-53 0.12079702088890087 1103 54-55 0.09099084424727977 1103 56-57 0.15527633197434199 1103 58-59 0.2199940895527348 1103 60-61 0.07931784924507923 1103 62-63 0.059892747629938015 1103 64-65 0.05698832431381362 1103 66-67 0.04145003850324258 1103 68-69 -0.010214024440337255 1103 70-71 0.07246506699061683 1103 72-73 0.13638528524864313 1103 74-75 0.1580962632129399 1103 76-77 0.10189766721359916 1103 78-79 0.11520069044428283 1103 80-81 0.09734424562020294 1103 82-83 0.1956080106701492 1103 84-85 0.32358422475400417 1103 86-87 0.29469205494701356 1103 88-89 0.19045541024845036 1103 90-91 0.23020114791588142 1103 92-93 0.09880453187523486 1103 94-95 0.08785167200083777 1103 96-97 0.26714281093376613 1103 98-99 0.3093294010769405 1103 100-101 0.3080032099959489 1104 1 0.31408905093307027 1104 2 0.2535688248028798 1104 3 0.22300912945794948 1104 4 0.20654830161189608 1104 5 0.2744836279157852 1104 6 0.2497507985658416 1104 7 0.225552441533587 1104 8 0.2791470553919382 1104 9 0.3986898699864909 1104 10-11 0.3823793942277689 1104 12-13 0.417779198396488 1104 14-15 0.43428437423788324 1104 16-17 0.44057339628459147 1104 18-19 0.351355199570186 1104 20-21 0.4990005428421256 1104 22-23 0.4738137690663322 1104 24-25 0.3922719338534435 1104 26-27 0.22123206436165077 1104 28-29 0.4714471700927376 1104 30-31 0.35457033405058525 1104 32-33 0.37829466784344135 1104 34-35 0.3741286776846877 1104 36-37 0.37357286429615755 1104 38-39 0.34136215965975225 1104 40-41 0.4970432325704195 1104 42-43 0.4691117284590547 1104 44-45 0.578584259675047 1104 46-47 0.4601770363453994 1104 48-49 0.3538770181688946 1104 50-51 0.29442045251598614 1104 52-53 0.43403733979550907 1104 54-55 0.5417837965493462 1104 56-57 0.5794514544979918 1104 58-59 0.35371354492651363 1104 60-61 0.30807250859484725 1104 62-63 0.355303719535776 1104 64-65 0.4193903916845585 1104 66-67 0.5072146032854619 1104 68-69 0.45588446040815 1104 70-71 0.32501260961503675 1104 72-73 0.4439722597775315 1104 74-75 0.26640543695491203 1104 76-77 0.48403855214492353 1104 78-79 0.46726288413517736 1104 80-81 0.4644332737143557 1104 82-83 0.5365205529494332 1104 84-85 0.3711561858225494 1104 86-87 0.4464574308012246 1104 88-89 0.33519293354519775 1104 90-91 0.4626673244879811 1104 92-93 0.28489852994246334 1104 94-95 0.05555590683206546 1104 96-97 0.36663287098760833 1104 98-99 0.43229481834759653 1104 100-101 0.4222798028032919 1105 1 0.20101185943713418 1105 2 0.148447035172115 1105 3 0.21174625239318345 1105 4 0.05717633135341771 1105 5 0.10747961972109721 1105 6 0.09144592842392996 1105 7 0.18434300423453465 1105 8 0.18019604038165937 1105 9 0.09400212456841217 1105 10-11 0.2672025205743438 1105 12-13 0.2917916890891803 1105 14-15 0.20810749237731585 1105 16-17 0.26695040487463473 1105 18-19 0.3285540819282744 1105 20-21 0.2829959796753485 1105 22-23 0.3718897903380949 1105 24-25 0.3031548347415409 1105 26-27 0.2495742005542425 1105 28-29 0.3048142517291055 1105 30-31 0.4357591997425345 1105 32-33 0.47467019914926567 1105 34-35 0.1370337599102598 1105 36-37 0.2606530941298715 1105 38-39 0.2569271600234089 1105 40-41 0.27453989240818544 1105 42-43 0.3625239040543775 1105 44-45 0.3908861741165097 1105 46-47 0.47245837634972077 1105 48-49 0.4151156134357592 1105 50-51 0.5063072274545632 1105 52-53 0.3674596497869089 1105 54-55 0.5024884611166769 1105 56-57 0.5400804860759294 1105 58-59 0.5545180705102766 1105 60-61 0.494232110192236 1105 62-63 0.29266467581977196 1105 64-65 0.23782551188087808 1105 66-67 0.2508284621357717 1105 68-69 0.4016541212355804 1105 70-71 0.3828738275368124 1105 72-73 0.32790498278306757 1105 74-75 0.2906087718249637 1105 76-77 0.49290643171286064 1105 78-79 0.4081707375421715 1105 80-81 0.4303211682192831 1105 82-83 0.38057282843407236 1105 84-85 0.24012072279441554 1105 86-87 0.27746941859467 1105 88-89 0.26547955215201924 1105 90-91 0.39012262319796065 1105 92-93 0.29318405671739 1105 94-95 0.24870289293172476 1105 96-97 0.3358815870663179 1105 98-99 0.3764303396463795 1105 100-101 0.3747074307348335 1106 1 0.1059328925448888 1106 2 0.16675849365786277 1106 3 0.20612780666967012 1106 4 0.24336861483341465 1106 5 0.18156155613441882 1106 6 0.2562167508365789 1106 7 0.18413954358469198 1106 8 0.22110058924058507 1106 9 0.19566024063951915 1106 10-11 0.33187409653791633 1106 12-13 0.27617625387748035 1106 14-15 0.32262475585736894 1106 16-17 0.33263521215980063 1106 18-19 0.26344518093881675 1106 20-21 0.24847282004412108 1106 22-23 0.29111204401972657 1106 24-25 0.341958811874683 1106 26-27 0.2817965853519411 1106 28-29 0.3758422424309913 1106 30-31 0.3812208192458755 1106 32-33 0.29207621023739705 1106 34-35 0.2648783067440874 1106 36-37 0.14467265132675067 1106 38-39 0.1656975220367798 1106 40-41 0.12566546821268076 1106 42-43 0.14653332215149106 1106 44-45 0.21173880046910654 1106 46-47 0.2729547578991074 1106 48-49 0.30967508380285125 1106 50-51 0.39671862516001255 1106 52-53 0.25390275043491073 1106 54-55 0.3608260315169147 1106 56-57 0.4186660558702613 1106 58-59 0.2975610464487133 1106 60-61 0.40644725510264124 1106 62-63 0.3174840926165885 1106 64-65 0.2454377769611895 1106 66-67 0.32663275606487474 1106 68-69 0.3439033970432561 1106 70-71 0.4237466832258008 1106 72-73 0.40656532674150725 1106 74-75 0.4132944578321087 1106 76-77 0.422165715504228 1106 78-79 0.4874893085996135 1106 80-81 0.48246654472192674 1106 82-83 0.47260816516484283 1106 84-85 0.5450407786395459 1106 86-87 0.46909725038279504 1106 88-89 0.3527948461221193 1106 90-91 0.37541319188065714 1106 92-93 0.26212156050089064 1106 94-95 0.2713978493607456 1106 96-97 0.31678940432581015 1106 98-99 0.24557385582102853 1106 100-101 0.17559960364842553 1107 1 0.057075745761427044 1107 2 0.07635921228636633 1107 3 0.2139333282516631 1107 4 0.05809054312705797 1107 5 0.0196499886105741 1107 6 0.11659938181226437 1107 7 0.09388483487687438 1107 8 0.12638025391052565 1107 9 0.15367919971073007 1107 10-11 0.09357067006763486 1107 12-13 0.06822285030604291 1107 14-15 0.1175241032751515 1107 16-17 -0.007642142993390166 1107 18-19 0.19086381429384858 1107 20-21 0.3986413292293136 1107 22-23 0.29746111170133105 1107 24-25 0.303376140009199 1107 26-27 0.259631534692609 1107 28-29 0.3081686339393741 1107 30-31 0.3341527416472374 1107 32-33 0.25026393090001875 1107 34-35 0.12628107945425882 1107 36-37 0.13711603030541397 1107 38-39 0.16690220075032158 1107 40-41 0.19813970244732104 1107 42-43 -0.10604500720551613 1107 44-45 0.11132200771619694 1107 46-47 0.17692492516869862 1107 48-49 0.17215464941738645 1107 50-51 0.16295626672133068 1107 52-53 0.16881556793036623 1107 54-55 0.18165387618389417 1107 56-57 0.16647019202265056 1107 58-59 0.2613772851980585 1107 60-61 0.23730207638362089 1107 62-63 0.29765667395518136 1107 64-65 -0.02144300962628165 1107 66-67 0.12991276064184376 1107 68-69 0.19462146464380936 1107 70-71 0.25408743720799976 1107 72-73 0.15588957924754965 1107 74-75 0.09647475354560697 1107 76-77 0.13363562826422992 1107 78-79 0.11999905919143572 1107 80-81 0.1688201473016342 1107 82-83 0.20205367641500516 1107 84-85 0.1945632681724021 1107 86-87 0.04794901810963381 1107 88-89 0.2941174153592421 1107 90-91 0.5384147335216518 1107 92-93 0.21354451448920386 1107 94-95 0.1892903220749318 1107 96-97 0.283151229738678 1107 98-99 0.2411615850654556 1107 100-101 0.5542614216854282 1108 1 -0.11106053794089377 1108 2 -0.004158852059191531 1108 3 0.03980818714539325 1108 4 -0.038285041456077806 1108 5 0.10614909397760641 1108 6 -0.046591737241385545 1108 7 -0.07335517030113436 1108 8 -0.05016344049030863 1108 9 -0.023377084825334293 1108 10-11 -0.08737104220831071 1108 12-13 -0.048005591562557015 1108 14-15 -0.07654378351510616 1108 16-17 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-1.5798788720998722 2316 90-91 -1.6002131462133171 2316 92-93 -1.6703447089127756 2316 94-95 -1.6318956118528192 2316 96-97 -1.7796738982749503 2316 98-99 -1.5659823012596021 2316 100-101 -1.5194282318523378 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27153.0 3 6088.0 4 5013.0 5 4202.0 6 4006.0 7 4329.0 8 5061.0 9 5853.0 10 7141.0 11 8395.0 12 10311.0 13 14595.0 14 15415.0 15 12787.0 16 14429.0 17 16846.0 18 15854.0 19 13210.0 20 13189.0 21 14007.0 22 14707.0 23 16373.0 24 18482.0 25 21619.0 26 25806.0 27 30985.0 28 36997.0 29 45056.0 30 55086.0 31 68790.0 32 86366.0 33 113276.0 34 157219.0 35 233320.0 36 386559.0 37 642513.0 38 630446.0 39 121561.0 40 103.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.771837991132756 17.484117482371964 12.067393487363464 24.676651039131812 2 16.19562517491705 18.913177506334648 39.19746518252615 25.693732136222145 3 20.804828147773073 22.283072059558105 27.67166405506935 29.240435737599473 4 12.88879841805281 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24-25 23.54140283871745 25.63013320367341 27.197686104135578 23.630777853473564 26-27 23.468158040003168 25.932591581055192 27.098539537284932 23.50071084165671 28-29 23.233048156593096 26.339734184803287 27.01280900229656 23.414408656307053 30-31 23.463855452603617 25.917697298033378 26.975443789917826 23.64300345944518 32-33 23.32339471395919 25.910167175116378 27.097908886884326 23.66852922404011 34-35 23.706426172208964 26.130036110184513 26.94665279770322 23.216884919903304 36-37 23.729594187461515 25.890890480607144 26.95286661376979 23.42664871816155 38-39 23.494853055026024 26.130955684942116 26.98744802230867 23.386743237723195 40-41 23.674305025221418 26.052734392792694 26.936373482198732 23.336587099787156 42-43 23.50376723199924 25.953881635064107 26.80554985053613 23.736801282400524 44-45 23.54648774287945 26.119095888028017 26.806046069185257 23.52837029990728 46-47 23.765747630495714 26.292915167148145 26.539101225773127 23.402235976583015 48-49 23.797242337069736 26.18428510692507 26.556779170177368 23.46169338582783 50-51 23.541959921811237 26.46043855925392 26.605688509350035 23.391913009584812 52-53 23.812700141124903 26.394104833334563 26.419152827664128 23.374042197876406 54-55 23.57637714282571 26.219585145091955 26.59613528164424 23.607902430438095 56-57 23.575303956526934 26.479192073367248 26.477026326713194 23.468477643392625 58-59 23.73915026827943 26.26353965906847 26.4988610349156 23.498449037736503 60-61 23.66967853798057 26.331069116061517 26.671294673832918 23.327957672125 62-63 23.476921321193732 26.36806813795199 26.762718546988012 23.392291993866266 64-65 23.429634841335176 26.46563604393655 26.556123374249168 23.548605740479108 66-67 23.478591575969414 26.526555444060655 26.42083101259155 23.574021967378375 68-69 23.495761801376233 26.283965324298258 26.65490869522616 23.565364179099348 70-71 23.524615620207552 26.23379730403501 26.48352235726701 23.75806471849043 72-73 23.611214241319185 26.34308188813535 26.5086918139366 23.53701205660886 74-75 23.423502717596378 26.703253121965297 26.32705121066568 23.546192949772642 76-77 23.620058395912043 26.378609502132484 26.32974284588199 23.67158925607348 78-79 23.596396315874962 26.4287070928087 26.435977713742076 23.53891887757426 80-81 23.593855496074774 26.491744577630673 26.366060292704123 23.54833963359043 82-83 23.671223062637896 26.574919951953984 26.368311080767228 23.385545904640896 84-85 23.60579814572232 26.64361782676621 26.18033201948987 23.5702520080216 86-87 23.5247242240242 26.576046272780797 26.356714666941688 23.542514836253314 88-89 23.6052056986044 26.562760822584686 26.17417116545781 23.6578623133531 90-91 23.489352965270623 26.602437449939142 26.274413103640633 23.633796481149606 92-93 23.617614563055717 26.543591954941952 26.388987751674563 23.449805730327768 94-95 23.532090151402254 26.50069708325104 26.466955881875197 23.500256883471504 96-97 23.538913448495226 26.366214260503057 26.482068088783006 23.612804202218715 98-99 23.436117882923966 26.46644017845054 26.231469536464353 23.865972402161145 100-101 23.50094898670216 26.49660229251817 26.079529295722125 23.922919425057547 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 63.0 1 57.5 2 120.0 3 195.0 4 159.5 5 165.5 6 330.0 7 592.5 8 940.5 9 1110.0 10 1081.0 11 1150.5 12 1363.5 13 1655.0 14 1929.5 15 2212.0 16 2539.0 17 2802.0 18 2951.5 19 3111.5 20 3258.5 21 3525.5 22 4020.0 23 4640.5 24 5802.5 25 7561.0 26 9971.0 27 13574.5 28 16738.0 29 19496.0 30 23420.0 31 28216.5 32 34112.0 33 38881.0 34 42720.0 35 48027.5 36 54625.0 37 60523.0 38 65154.0 39 69621.5 40 74539.5 41 79557.0 42 83889.5 43 89732.0 44 95760.5 45 100680.5 46 105711.5 47 112307.5 48 120805.0 49 122035.0 50 117000.0 51 114881.5 52 114367.5 53 114108.5 54 114354.0 55 109125.5 56 99909.5 57 91534.5 58 85230.5 59 78551.0 60 68578.5 61 57543.5 62 46683.0 63 36534.0 64 27248.0 65 20330.5 66 15088.0 67 11551.0 68 8935.5 69 6804.0 70 5208.5 71 3830.5 72 2993.0 73 2569.0 74 2059.0 75 1500.0 76 1092.5 77 677.5 78 384.5 79 231.0 80 159.5 81 108.5 82 78.0 83 52.0 84 41.0 85 38.0 86 36.5 87 32.5 88 26.0 89 22.0 90 20.5 91 26.5 92 28.5 93 25.0 94 24.5 95 28.0 96 28.5 97 25.5 98 17.5 99 9.5 100 8.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04727779777144366 2 0.01142603795634022 3 0.009852392010257435 4 0.00574722867265017 5 0.008210326675214529 6 0.005439341422329626 7 0.005439341422329626 8 0.00951029506545683 9 0.014915426793306395 10-11 0.0021552107522438135 12-13 0.008535318772775104 14-15 0.00831295575865471 16-17 0.019191638603313962 18-19 0.011135255553259704 20-21 0.0036091227676463868 22-23 0.009955021093697615 24-25 0.0215863172169182 26-27 0.00699588252117238 28-29 0.01765220235171124 30-31 0.014539120154025729 32-33 0.012811530582782671 34-35 0.2656553824849101 36-37 0.46913464525231025 38-39 0.4654742079429437 40-41 0.4861710731033803 42-43 0.47607921323176244 44-45 0.49087490609438866 46-47 0.49862340189412235 48-49 0.5056021795680548 50-51 0.5151124746335115 52-53 0.5039601142330119 54-55 0.49166172906743005 56-57 0.4864789603537009 58-59 0.4839816526566565 60-61 0.4805948929031305 62-63 0.478627835470527 64-65 0.494449819167555 66-67 0.4685872901406292 68-69 0.49545900515471675 70-71 0.47717392345512444 72-73 0.4859145003947799 74-75 0.4995812733395641 76-77 0.4861710731033803 78-79 0.48511057257449847 80-81 0.488240759619424 82-83 0.48817234023046385 84-85 0.4875736705770628 86-87 0.4895578328569063 88-89 0.5022496295090089 90-91 0.5280950536886945 92-93 0.5157795636758727 94-95 0.4871973639377821 96-97 0.4600177616733741 98-99 0.4519100640815997 100-101 0.4606506410212552 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2923148.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.42565915934193 #Duplication Level Percentage of deduplicated Percentage of total 1 72.0118370535117 37.75268024805474 2 14.579123283614864 15.286402962176377 3 5.500693540537566 8.651324540886487 4 2.6960285098192265 5.6536428695860526 5 1.4882346359765997 3.9010840887418263 6 0.9342922070010125 2.938853087967865 7 0.5931421430941677 2.176710747882752 8 0.41725565911203794 1.7499922373531411 9 0.2988830244845859 1.4102225613127934 >10 1.3530534446379734 12.397149651913333 >50 0.0868520648367575 3.1325597017328586 >100 0.03805815007660744 3.553168518218422 >500 0.0017628115132369308 0.6519132285312671 >1k 7.834717835389815E-4 0.7442955556420516 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3943 0.1348888253348787 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3322 0.1136446050627611 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0262908344018161E-4 2 0.0 0.0 0.0 0.0 1.0262908344018161E-4 3 0.0 0.0 0.0 3.420969448006054E-5 1.0262908344018161E-4 4 0.0 0.0 0.0 3.420969448006054E-5 1.0262908344018161E-4 5 0.0 0.0 0.0 3.420969448006054E-5 1.0262908344018161E-4 6 0.0 0.0 0.0 3.420969448006054E-5 2.0525816688036323E-4 7 0.0 0.0 0.0 3.420969448006054E-5 2.0525816688036323E-4 8 0.0 0.0 0.0 3.420969448006054E-5 2.3946786136042376E-4 9 0.0 0.0 0.0 3.420969448006054E-5 2.3946786136042376E-4 10-11 0.0 0.0 0.0 5.131454172009081E-5 2.736775558404843E-4 12-13 0.0 0.0 0.0 1.0262908344018161E-4 5.302502644409383E-4 14-15 0.0 6.841938896012108E-5 0.0 1.3683877792024216E-4 6.499841951211503E-4 16-17 0.0 1.0262908344018161E-4 0.0 1.5394362516027243E-4 7.01298736841241E-4 18-19 3.420969448006054E-5 1.0262908344018161E-4 0.0 1.8815331964033296E-4 7.184035840812712E-4 20-21 3.420969448006054E-5 1.0262908344018161E-4 0.0 2.56572708600454E-4 9.065569037216043E-4 22-23 3.420969448006054E-5 1.0262908344018161E-4 0.0 5.302502644409383E-4 0.0010947102233619373 24-25 3.420969448006054E-5 1.3683877792024216E-4 0.0 6.499841951211502E-4 0.0010947102233619373 26-27 3.420969448006054E-5 1.710484724003027E-4 0.0 7.355084313213015E-4 0.0011631296123220582 28-29 3.420969448006054E-5 1.710484724003027E-4 0.0 0.0011631296123220582 0.0011631296123220582 30-31 3.420969448006054E-5 1.710484724003027E-4 0.0 0.0028907191835651153 0.0011973393068021188 32-33 3.420969448006054E-5 1.710484724003027E-4 0.0 0.005866962603330382 0.0013512829319623912 34-35 6.841938896012108E-5 1.710484724003027E-4 0.0 0.009202407815136284 0.0014881217098826334 36-37 6.841938896012108E-5 1.8815331964033296E-4 0.0 0.01652328243386924 0.0015052265571226637 38-39 6.841938896012108E-5 2.0525816688036323E-4 0.0 0.028513780349130456 0.0016249604878028755 40-41 6.841938896012108E-5 2.223630141203935E-4 0.0 0.0413082060846731 0.0016420653350429058 42-43 6.841938896012108E-5 2.3946786136042376E-4 0.0 0.054136841514695805 0.001727589571243057 44-45 6.841938896012108E-5 2.3946786136042376E-4 0.0 0.07030092215652439 0.0018302186546832388 46-47 6.841938896012108E-5 2.56572708600454E-4 0.0 0.0902964885801198 0.0019670574326034807 48-49 6.841938896012108E-5 3.0788725032054485E-4 0.0 0.11133545068535702 0.002086791363283693 50-51 6.841938896012108E-5 3.420969448006054E-4 0.0 0.13600064040548066 0.002086791363283693 52-53 6.841938896012108E-5 3.420969448006054E-4 0.0 0.16049478165320402 0.002360468919124177 54-55 6.841938896012108E-5 3.420969448006054E-4 0.0 0.18764017422313206 0.0024288883080842982 56-57 6.841938896012108E-5 3.420969448006054E-4 0.0 0.2194209803951083 0.002480202849804389 58-59 6.841938896012108E-5 3.420969448006054E-4 0.0 0.25585430501637274 0.0025315173915244796 60-61 6.841938896012108E-5 3.420969448006054E-4 0.0 0.2980348583102874 0.00256572708600454 62-63 6.841938896012108E-5 3.420969448006054E-4 0.0 0.34071145217416293 0.0026341464749646613 64-65 6.841938896012108E-5 3.420969448006054E-4 0.0 0.3861590312909233 0.002719670711164813 66-67 6.841938896012108E-5 3.763066392806659E-4 0.0 0.4399537758608185 0.0027880901001249335 68-69 6.841938896012108E-5 3.763066392806659E-4 0.0 0.5031390815654904 0.0028394046418450244 70-71 6.841938896012108E-5 3.763066392806659E-4 0.0 0.5740215685281758 0.0029078240308051456 72-73 6.841938896012108E-5 3.763066392806659E-4 0.0 0.6448014264074211 0.0031986064338856606 74-75 6.841938896012108E-5 3.763066392806659E-4 0.0 0.7179588580530305 0.003455179142486114 76-77 6.841938896012108E-5 3.763066392806659E-4 0.0 0.8005239556806567 0.0034893888369661747 78-79 6.841938896012108E-5 3.763066392806659E-4 0.0 0.9008267798961941 0.003557808225926296 80-81 6.841938896012108E-5 3.763066392806659E-4 0.0 1.0102122779961877 0.003626227614886417 82-83 6.841938896012108E-5 3.763066392806659E-4 0.0 1.1255160532412316 0.00383148578176678 84-85 6.841938896012108E-5 3.763066392806659E-4 0.0 1.240460626694235 0.0038999051707269013 86-87 6.841938896012108E-5 3.763066392806659E-4 0.0 1.364145777086894 0.004224897268287476 88-89 6.841938896012108E-5 3.763066392806659E-4 0.0 1.50886988958479 0.00448146997688793 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 9225 0.0 26.781225 2 GTACATG 9560 0.0 26.643082 1 GTATCAA 9400 0.0 24.729406 1 ACATGGG 9490 0.0 24.637798 3 TCAACGC 10260 0.0 22.142548 4 CAACGCA 10270 0.0 22.028818 5 ATCAACG 10390 0.0 21.82032 3 CATGGGG 5980 0.0 21.76533 4 AACGCAG 10530 0.0 21.52874 6 GTACCTG 2545 0.0 21.206896 1 GAGTACA 7000 0.0 21.169294 1 GTGTAGC 2270 0.0 21.064714 1 TAGATCG 135 3.8911088E-4 21.035421 5 TATCAAC 10945 0.0 20.756388 2 GGTATCA 7060 0.0 20.72115 1 TAGCCCT 1995 0.0 20.640018 4 TACCTGG 2535 0.0 20.53719 2 AGTACAT 6695 0.0 19.8647 2 GTATAGG 755 0.0 19.43904 1 GTGTAGG 1415 0.0 19.07122 1 >>END_MODULE