FastQCFastQC Report
Thu 26 May 2016
SRR936368_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936368_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2923148
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41080.14053342492408868No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34810.11908394648509073No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32000.10947102233619373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG1754.8803486E-927.1885641
TACATGG89050.024.7214092
GTACATG91550.024.5305881
GTATCAA99250.023.586251
ACATGGG90900.022.2303223
GAGTACA72250.022.1271651
TCAACGC108450.021.1697524
CAACGCA109300.021.0051215
GGTATCA74000.020.9609131
ATCAACG109900.020.8472823
AGTACAT68600.020.679322
AACGCAG112500.020.4498086
TATCAAC114250.020.2652852
GTGTAGC21550.018.5462591
CATGGGG56750.018.472534
AGAGTAC93650.018.08289110-11
GAGTACT60150.018.02013412-13
ACGCAGA129250.017.7996397
GTACTTT60950.017.5890414-15
CGCAGAG131000.017.4894378
Produced by FastQC (version 0.11.5)