FastQCFastQC Report
Thu 26 May 2016
SRR936367_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936367_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3604331
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53150.14746148453069377No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36920.10243232377936433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38200.044.0072671
GTATCAA69900.039.699021
ATCAACG83350.031.9777343
TCAACGC84500.031.5972424
CAACGCA84700.031.4667425
TACATGG129750.030.7941272
GTACATG133450.030.4813351
AACGCAG89500.029.9894666
TATCAAC90700.029.5943372
ACATGGG133450.028.7350063
GAGTACA96150.025.6594771
AGTACAT92700.024.7682192
ACGCAGA109800.024.4021917
CATGGGG76650.024.2103314
CGCAGAG113300.023.6490298
GTGTAGC21550.021.7544081
CATGGGA54200.021.0496354
TATAGCG1150.003798395920.582545
GCAGAGT129450.019.8937939
TAACGCA3204.1654857E-1019.231814
Produced by FastQC (version 0.11.5)