##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936367_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3604331 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.722837885865644 33.0 31.0 34.0 30.0 34.0 2 31.805968708201327 34.0 31.0 34.0 30.0 34.0 3 31.886863609363292 34.0 31.0 34.0 30.0 34.0 4 35.37773417591226 37.0 35.0 37.0 33.0 37.0 5 35.36476200437751 37.0 35.0 37.0 33.0 37.0 6 35.45357765421655 37.0 35.0 37.0 33.0 37.0 7 35.39361423798203 37.0 35.0 37.0 33.0 37.0 8 35.39565900024165 37.0 35.0 37.0 33.0 37.0 9 37.06810695244138 39.0 37.0 39.0 33.0 39.0 10-11 36.99036423125401 39.0 37.0 39.0 33.0 39.0 12-13 36.8944496218577 39.0 37.0 39.0 33.0 39.0 14-15 38.15213488994213 40.0 38.0 41.0 33.0 41.0 16-17 38.0979331531982 40.0 38.0 41.0 33.0 41.0 18-19 38.03621448751516 40.0 38.0 41.0 33.0 41.0 20-21 37.94867660600539 40.0 38.0 41.0 33.0 41.0 22-23 37.789264498737765 40.0 38.0 41.0 32.5 41.0 24-25 37.94894544923871 40.0 38.0 41.0 33.0 41.0 26-27 37.90226105759987 40.0 38.0 41.0 33.0 41.0 28-29 37.772332785196475 40.0 38.0 41.0 32.5 41.0 30-31 37.633326267759536 40.0 38.0 41.0 32.0 41.0 32-33 37.46113356403727 40.0 37.0 41.0 32.0 41.0 34-35 37.20494885181189 40.0 37.0 41.0 31.0 41.0 36-37 37.01237788649267 40.0 37.0 41.0 31.0 41.0 38-39 36.845544429743 40.0 37.0 41.0 30.0 41.0 40-41 36.669357226070524 40.0 36.0 41.0 30.0 41.0 42-43 36.48119151653941 40.0 36.0 41.0 30.0 41.0 44-45 36.308955115387576 40.0 36.0 41.0 30.0 41.0 46-47 36.06023822451378 39.5 35.5 41.0 28.5 41.0 48-49 35.91062502306253 39.0 35.0 41.0 28.0 41.0 50-51 35.60909888686694 39.0 35.0 40.5 28.0 41.0 52-53 35.628482927899796 39.0 35.0 40.5 28.0 41.0 54-55 35.710370246239876 39.0 35.0 41.0 28.0 41.0 56-57 35.54660587498762 39.0 35.0 41.0 28.0 41.0 58-59 35.30834723558963 39.0 35.0 41.0 27.5 41.0 60-61 35.013823785884256 38.0 35.0 40.5 27.0 41.0 62-63 34.70868199951669 37.5 34.0 40.0 26.0 41.0 64-65 34.37731648397442 37.0 34.0 40.0 26.0 41.0 66-67 34.025828232756645 36.5 34.0 39.5 26.0 41.0 68-69 33.647124528796056 36.0 34.0 39.0 25.5 41.0 70-71 33.24967337905426 35.5 33.0 39.0 24.5 41.0 72-73 32.85608799524793 35.0 33.0 38.0 24.0 40.0 74-75 32.449913035179065 35.0 33.0 37.0 22.5 39.5 76-77 32.061691614893306 35.0 32.5 37.0 21.5 39.0 78-79 31.67888493037959 35.0 32.0 36.0 20.5 39.0 80-81 31.29438694725873 35.0 32.0 36.0 19.5 37.5 82-83 30.95930853742345 35.0 31.5 35.5 18.0 37.0 84-85 30.647585779441457 35.0 31.0 35.0 16.0 37.0 86-87 30.339461747547602 34.0 31.0 35.0 11.0 36.0 88-89 30.0231936245589 34.0 31.0 35.0 7.0 36.0 90-91 29.67282513731397 34.0 30.5 35.0 2.0 36.0 92-93 29.373729965422154 34.0 30.0 35.0 2.0 35.0 94-95 29.006715531953084 34.0 30.0 35.0 2.0 35.0 96-97 28.591781248725493 34.0 29.0 35.0 2.0 35.0 98-99 28.105174164082044 34.0 29.0 35.0 2.0 35.0 100-101 24.867877561744468 30.5 23.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2570896942813867 1101 2 0.22248142026408857 1101 3 0.2762275132010039 1101 4 0.10000633345718768 1101 5 0.34730194176741946 1101 6 0.29387972521114847 1101 7 0.32975664160580465 1101 8 0.22444168684138788 1101 9 0.3080292305945562 1101 10-11 0.49065211990473045 1101 12-13 0.3943358780797226 1101 14-15 0.5952714431175181 1101 16-17 0.5715422525606471 1101 18-19 0.689161895841643 1101 20-21 0.6293807516123877 1101 22-23 0.5667475387819465 1101 24-25 0.4621756076688257 1101 26-27 0.4759865124207181 1101 28-29 0.43483744780532874 1101 30-31 0.5100179042252648 1101 32-33 0.5888462009190505 1101 34-35 0.39253188311615617 1101 36-37 0.2750432907161837 1101 38-39 0.35350576108833565 1101 40-41 0.31522923420753557 1101 42-43 0.3186403235962274 1101 44-45 0.40168656535900027 1101 46-47 0.2475197149993278 1101 48-49 0.26119005146362895 1101 50-51 0.42380603086837 1101 52-53 0.5355147709557357 1101 54-55 0.5093802406104615 1101 56-57 0.4033172068372721 1101 58-59 0.43956898773183894 1101 60-61 0.324192740948682 1101 62-63 0.36719478152395624 1101 64-65 0.30667504517042943 1101 66-67 0.398230250135704 1101 68-69 0.35304583958744473 1101 70-71 0.44621967554010666 1101 72-73 0.4648441310879292 1101 74-75 0.34923748187072334 1101 76-77 0.267202429010851 1101 78-79 0.4007755548082237 1101 80-81 0.3110747645776506 1101 82-83 0.4501543636691565 1101 84-85 0.47777767762743295 1101 86-87 0.4444492657058774 1101 88-89 0.2901869330532314 1101 90-91 0.33992217910740763 1101 92-93 0.4137714640333634 1101 94-95 0.6418055675454788 1101 96-97 0.6792692782342264 1101 98-99 0.6068176660121658 1101 100-101 0.7335323665825264 1102 1 0.19039356473421876 1102 2 0.16936059261821157 1102 3 0.09381954637181522 1102 4 0.15061204221143498 1102 5 0.23727983839124533 1102 6 0.12261854817114681 1102 7 0.12657920876335282 1102 8 0.10599282669434729 1102 9 0.1586321215205757 1102 10-11 0.21876018444626055 1102 12-13 0.24796648400394616 1102 14-15 0.2581726832105673 1102 16-17 0.22170970614728702 1102 18-19 0.34034211004631487 1102 20-21 0.2824155526198382 1102 22-23 0.2573602548119851 1102 24-25 0.36245277008264765 1102 26-27 0.25504702362449194 1102 28-29 0.2461330852270791 1102 30-31 0.21360713365698558 1102 32-33 0.03541437620122423 1102 34-35 0.01580917481856403 1102 36-37 0.11092582623117409 1102 38-39 0.06804256399698971 1102 40-41 0.15751507718985636 1102 42-43 0.12101421956698744 1102 44-45 -0.009592247970651613 1102 46-47 0.12459496261597991 1102 48-49 0.0695061463183535 1102 50-51 0.0024580398555897887 1102 52-53 0.10119513558270654 1102 54-55 0.015429040422823448 1102 56-57 0.09345368917654184 1102 58-59 0.10248483494918759 1102 60-61 0.09162194094238174 1102 62-63 0.1837326154262442 1102 64-65 0.025890686106180283 1102 66-67 0.12774070220651623 1102 68-69 0.0792594326250935 1102 70-71 0.20525692722935673 1102 72-73 0.09207197653502419 1102 74-75 0.1280895566625233 1102 76-77 0.09945162365067972 1102 78-79 0.06830839286680757 1102 80-81 0.10809292216552535 1102 82-83 0.25945255454055527 1102 84-85 0.20754308099540353 1102 86-87 0.05736316530665775 1102 88-89 -0.00590154841084356 1102 90-91 -0.013756288458147026 1102 92-93 -0.02181059982691025 1102 94-95 0.09229238881146173 1102 96-97 0.16620719401928952 1102 98-99 0.17115925143835753 1102 100-101 0.3739032052096469 1103 1 0.22641158858614574 1103 2 0.17252016306773044 1103 3 0.2567339420691148 1103 4 0.11326561370424315 1103 5 0.09692583698819135 1103 6 0.1001328035630138 1103 7 0.17492882112462382 1103 8 0.1306892812839564 1103 9 0.2115470568541582 1103 10-11 0.27812062550898276 1103 12-13 0.3109995996839885 1103 14-15 0.317120302515157 1103 16-17 0.37795329796639976 1103 18-19 0.30128143693999476 1103 20-21 0.42491730643776293 1103 22-23 0.44187674070050065 1103 24-25 0.4110456684691357 1103 26-27 0.3738956196707548 1103 28-29 0.36643060469896227 1103 30-31 0.3537079557029301 1103 32-33 0.37907544863840315 1103 34-35 0.2675009345724959 1103 36-37 0.282487443701001 1103 38-39 0.38193460076421815 1103 40-41 0.46070927430100994 1103 42-43 0.2376197274546712 1103 44-45 0.40886870934411235 1103 46-47 0.39804530708381236 1103 48-49 0.4388363677491043 1103 50-51 0.5038386093517389 1103 52-53 0.5772132493740045 1103 54-55 0.5168842596050922 1103 56-57 0.5769093400678429 1103 58-59 0.5312060768321558 1103 60-61 0.5740329459250191 1103 62-63 0.5619322089002381 1103 64-65 0.4463240140431495 1103 66-67 0.5033878160517915 1103 68-69 0.45538142640128143 1103 70-71 0.43101421681617325 1103 72-73 0.5127688268579078 1103 74-75 0.5346065641994429 1103 76-77 0.4510134209992671 1103 78-79 0.4431545965255417 1103 80-81 0.5186239242701056 1103 82-83 0.591197108711043 1103 84-85 0.5676221377111759 1103 86-87 0.6274385345078137 1103 88-89 0.5532983508570481 1103 90-91 0.5965201851986066 1103 92-93 0.5429641919634207 1103 94-95 0.5866693787202628 1103 96-97 0.693360792493916 1103 98-99 0.7045141702230957 1103 100-101 0.8781613851963321 1104 1 0.24702987597754245 1104 2 0.24375017297595747 1104 3 0.25455871206700564 1104 4 0.3277243234700222 1104 5 0.2853336429018398 1104 6 0.29866757424809265 1104 7 0.29458148223819336 1104 8 0.16710966524382798 1104 9 0.27783542688889185 1104 10-11 0.3487838240503791 1104 12-13 0.33978070181286313 1104 14-15 0.44882274945396006 1104 16-17 0.4026189011916159 1104 18-19 0.18762990944915003 1104 20-21 0.30267159297918766 1104 22-23 0.37438300706089933 1104 24-25 0.41032684638405925 1104 26-27 0.3016748351901768 1104 28-29 0.3786596452281614 1104 30-31 0.34769307824481643 1104 32-33 0.3976007740919343 1104 34-35 0.38142616908277205 1104 36-37 0.3926138236283734 1104 38-39 0.2452801329698744 1104 40-41 0.20089142398307303 1104 42-43 0.21604624350449342 1104 44-45 0.2409163604885336 1104 46-47 0.27545156267333937 1104 48-49 0.242699457704461 1104 50-51 0.197207549940849 1104 52-53 0.16578388562098922 1104 54-55 0.2475171729716621 1104 56-57 0.39355366081776566 1104 58-59 0.217077950170534 1104 60-61 0.26737171830810524 1104 62-63 0.31178928763340963 1104 64-65 0.22286474916288768 1104 66-67 0.2997447249364953 1104 68-69 0.35450411157631834 1104 70-71 0.26679770559618277 1104 72-73 0.2980899233040546 1104 74-75 0.28216520578214244 1104 76-77 0.33854766483732845 1104 78-79 0.2806912041083329 1104 80-81 0.3796400225901877 1104 82-83 0.50819316442524 1104 84-85 0.28071543154804246 1104 86-87 0.2720536072063702 1104 88-89 0.41516023115994116 1104 90-91 0.3940548238064068 1104 92-93 0.29042453100726817 1104 94-95 0.10883633317894592 1104 96-97 0.3170117400143937 1104 98-99 0.32181176178652393 1104 100-101 0.4003884811190481 1105 1 0.25070027450867016 1105 2 0.227347313758699 1105 3 0.18927598021565828 1105 4 0.10215775798115345 1105 5 0.10682278272653178 1105 6 0.12049020742409056 1105 7 0.21210422001581009 1105 8 0.20333387830702065 1105 9 0.17197701340652571 1105 10-11 0.21001960657252994 1105 12-13 0.2123744585581946 1105 14-15 0.20298064259468873 1105 16-17 0.24045240745265062 1105 18-19 0.182328379163458 1105 20-21 0.22621026163655245 1105 22-23 0.27244407934529846 1105 24-25 0.2473700428802701 1105 26-27 0.27480542985332335 1105 28-29 0.20660298928955712 1105 30-31 0.31912700670542904 1105 32-33 0.40630540122768366 1105 34-35 0.1721643958614436 1105 36-37 0.22615130271344697 1105 38-39 0.3359349300773786 1105 40-41 0.3476635191146755 1105 42-43 0.3778398105567007 1105 44-45 0.3838274626342937 1105 46-47 0.3982126528999075 1105 48-49 0.3840228047646619 1105 50-51 0.2992624773849215 1105 52-53 0.3703779724796661 1105 54-55 0.4413189661932009 1105 56-57 0.5341538286723306 1105 58-59 0.5172405094015105 1105 60-61 0.4567567432360775 1105 62-63 0.39882382287915874 1105 64-65 0.37916281147450803 1105 66-67 0.44008604111044036 1105 68-69 0.4446058561540269 1105 70-71 0.3772663923825519 1105 72-73 0.37833366262830026 1105 74-75 0.38422816072665 1105 76-77 0.45722083262138113 1105 78-79 0.333516100288346 1105 80-81 0.36114292464895925 1105 82-83 0.4275113395218284 1105 84-85 0.38258281592645105 1105 86-87 0.42216641001800426 1105 88-89 0.30833861151455366 1105 90-91 0.4782433988987904 1105 92-93 0.39199208120285434 1105 94-95 0.2900106334977828 1105 96-97 0.29091259916349443 1105 98-99 0.3276750293247126 1105 100-101 0.39521909945158384 1106 1 0.1688341572721761 1106 2 0.15261606826719643 1106 3 0.14713840090771058 1106 4 0.18531334871801164 1106 5 0.2069255859274577 1106 6 0.09045273862196979 1106 7 0.15838475578188138 1106 8 0.1265995019663393 1106 9 0.14707298724338358 1106 10-11 0.1980519185554357 1106 12-13 0.16330308189633058 1106 14-15 0.19518052361598137 1106 16-17 0.1586910817696321 1106 18-19 0.12354089983740124 1106 20-21 0.20755436467548805 1106 22-23 0.17108933055931885 1106 24-25 0.14447942609510278 1106 26-27 0.11937317902569333 1106 28-29 0.1731602876092566 1106 30-31 0.28041705163323627 1106 32-33 0.3035760469998152 1106 34-35 0.24348655716126189 1106 36-37 0.3007918632067472 1106 38-39 0.3560067221437109 1106 40-41 0.3946062389767633 1106 42-43 0.25989969364515275 1106 44-45 0.22504622601873336 1106 46-47 0.31696064103642385 1106 48-49 0.41116905669322534 1106 50-51 0.4427722707377697 1106 52-53 0.3628165901554823 1106 54-55 0.36974467820702017 1106 56-57 0.4009471498140158 1106 58-59 0.46577630270312653 1106 60-61 0.4980552735927475 1106 62-63 0.40551091225524516 1106 64-65 0.42203263295806437 1106 66-67 0.4841410819334726 1106 68-69 0.4548834892836311 1106 70-71 0.5191164487460753 1106 72-73 0.6701123532569468 1106 74-75 0.6394369726752487 1106 76-77 0.6248415950345603 1106 78-79 0.5752464345411532 1106 80-81 0.6357989889182463 1106 82-83 0.6250208473400853 1106 84-85 0.6377810591542747 1106 86-87 0.638178075736171 1106 88-89 0.5390749235433141 1106 90-91 0.6020092096602951 1106 92-93 0.644424946986728 1106 94-95 0.5751841871190813 1106 96-97 0.583835129902802 1106 98-99 0.5727717250589457 1106 100-101 0.5771638372228125 1107 1 0.07362109624085278 1107 2 0.13777298942544292 1107 3 0.24458195129611582 1107 4 0.1819955190529896 1107 5 0.10303147520068734 1107 6 0.20864690913850836 1107 7 0.1265920041467794 1107 8 0.2216616933672526 1107 9 0.21790953423006698 1107 10-11 0.19869632348729027 1107 12-13 0.14295177331157305 1107 14-15 0.298033801319157 1107 16-17 0.06566460721897727 1107 18-19 0.25491393253276584 1107 20-21 0.32940679817843943 1107 22-23 0.26507497134226554 1107 24-25 0.34871802557845655 1107 26-27 0.2828929768045825 1107 28-29 0.34833481084448437 1107 30-31 0.3106744117830402 1107 32-33 0.4026844651531363 1107 34-35 0.3079025875152581 1107 36-37 0.36776225964599973 1107 38-39 0.36903536579410456 1107 40-41 0.47141832089953084 1107 42-43 0.04735619538210756 1107 44-45 0.3962825124158016 1107 46-47 0.4027543027260734 1107 48-49 0.36007141037107715 1107 50-51 0.3550262332514009 1107 52-53 0.34420995583555936 1107 54-55 0.345722270625366 1107 56-57 0.3271295789155175 1107 58-59 0.40657132054518996 1107 60-61 0.44086703331304733 1107 62-63 0.45152419622389317 1107 64-65 0.25781680716170285 1107 66-67 0.3155727324100255 1107 68-69 0.4095712561191789 1107 70-71 0.4995078614922761 1107 72-73 0.27136568736415967 1107 74-75 0.1755170448247938 1107 76-77 0.25567895682886643 1107 78-79 0.3438114913488697 1107 80-81 0.36611682714060834 1107 82-83 0.4263964211934663 1107 84-85 0.43089619519179934 1107 86-87 0.1924166380137038 1107 88-89 0.29959142972264274 1107 90-91 0.603831086017145 1107 92-93 0.3314720357981571 1107 94-95 0.45100136661227097 1107 96-97 0.6604466724154818 1107 98-99 0.6794350655246362 1107 100-101 0.6489352736393563 1108 1 -0.18147528501356902 1108 2 -0.01853240332985706 1108 3 0.013789593322758975 1108 4 -0.034512501831493125 1108 5 -0.056568944651289144 1108 6 -0.0826973829919666 1108 7 -0.054543938695694294 1108 8 0.011711562050891189 1108 9 -0.02790352352661074 1108 10-11 -0.10785296181833814 1108 12-13 -0.07114944164633386 1108 14-15 -0.1476452371657686 1108 16-17 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-1.5416006724072702 2316 50-51 -1.5520907624383753 2316 52-53 -1.6045455282384893 2316 54-55 -1.576192717589926 2316 56-57 -1.5326423800422617 2316 58-59 -1.6404909680840234 2316 60-61 -1.8306521951068717 2316 62-63 -1.7089428442436017 2316 64-65 -1.4903730594857123 2316 66-67 -1.6341638340414093 2316 68-69 -1.666384036479485 2316 70-71 -1.476964142573209 2316 72-73 -1.3616070717064446 2316 74-75 -1.3582695856515556 2316 76-77 -1.7532031789998612 2316 78-79 -1.6894695126523658 2316 80-81 -1.6026818170154016 2316 82-83 -1.6043465170405007 2316 84-85 -1.8170480641361273 2316 86-87 -1.921550714193927 2316 88-89 -1.9113771763147298 2316 90-91 -2.024124906161674 2316 92-93 -2.079718829037276 2316 94-95 -1.9482716842104644 2316 96-97 -2.0138504104807247 2316 98-99 -1.934060097014612 2316 100-101 -2.0224660066973925 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28386.0 3 6392.0 4 5459.0 5 4644.0 6 4478.0 7 4787.0 8 5524.0 9 6742.0 10 7736.0 11 9621.0 12 12572.0 13 17829.0 14 18183.0 15 15410.0 16 17560.0 17 20736.0 18 19467.0 19 15599.0 20 15418.0 21 15605.0 22 17285.0 23 18875.0 24 22189.0 25 25511.0 26 30657.0 27 36848.0 28 44694.0 29 54147.0 30 66773.0 31 83518.0 32 105593.0 33 138879.0 34 193206.0 35 288135.0 36 484579.0 37 800085.0 38 782323.0 39 158676.0 40 210.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.25772279596719 17.617079224547734 11.350351935771652 24.774846043713424 2 16.624888177566255 19.395060056693698 38.132948271996554 25.847103493743496 3 20.73471550896509 22.57174013174324 27.880426639437513 28.81311771985416 4 12.832506137379637 14.440266537630016 35.79829218810347 36.92893513688687 5 14.221445318461177 37.199123208612534 33.69867232696549 14.880759145960795 6 35.11291891119506 34.2891706331755 16.44383465103277 14.154075804596664 7 30.893830722916636 31.059889294285753 19.40901460816004 18.637265374637572 8 28.087807869300956 34.20697252084833 18.98451861193311 18.720700997917593 9 27.07075864365392 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27.43921718738142 23.567760992919705 34-35 23.448522287596663 26.268238954258692 27.20214518170036 23.081093576444285 36-37 23.495743536840617 26.01252145234576 27.21429879504505 23.277436215768578 38-39 23.17036035266394 26.375932468559782 27.18028698715884 23.27342019161744 40-41 23.452904128000508 26.20009537338709 27.145674694228767 23.201325804383636 42-43 23.324900416925505 26.130690737669493 27.08330690932801 23.461101936076997 44-45 23.315243248698724 26.305285965386975 27.20385170898118 23.175619076933113 46-47 23.423971899191304 26.528028872588326 26.800075616559354 23.24792361166101 48-49 23.532420809950015 26.45830765652998 26.74437280655036 23.264898726969648 50-51 23.415982532495356 26.612316130154046 26.73718162610153 23.234519711249064 52-53 23.66741702490626 26.521218184713046 26.62461202909862 23.186752761282072 54-55 23.48805133989933 26.52265469523765 26.80145769359662 23.187836271266395 56-57 23.240903302878007 26.785379490499935 26.730067836231225 23.24364937039084 58-59 23.38476691382626 26.5002351533487 26.7663055220474 23.348692410777634 60-61 23.25540421967159 26.584809154400002 26.94631010467289 23.213476521255515 62-63 23.315906760681326 26.603585707348433 26.951401661778046 23.1291058701922 64-65 23.084522850111615 26.58942736325628 26.925618938364554 23.400430848267554 66-67 23.11520215563551 26.78204865371035 26.81174801285365 23.29100117780049 68-69 23.413298024574775 26.465565108121226 26.955089372506986 23.166047494797017 70-71 23.15586126152495 26.388924895291062 26.86999365822723 23.585220184956757 72-73 23.26736206362057 26.611994482321794 26.811937833564166 23.30870562049347 74-75 23.160830445196027 26.749332916958267 26.659342926689177 23.430493711156526 76-77 23.31367190576454 26.61385053830215 26.741186334715415 23.331291221217892 78-79 23.37616645746111 26.75360139058387 26.63369559232586 23.236536559629158 80-81 23.29408173207942 26.704280364464882 26.607818305627205 23.39381959782849 82-83 23.3913232692546 26.919033916466667 26.5160274893568 23.17361532492193 84-85 23.360690243903797 26.880464667878112 26.443458512712624 23.315386575505464 86-87 23.279855359948254 26.722528357172827 26.63385686824178 23.36375941463714 88-89 23.33011045391015 26.7833967104323 26.5172059238708 23.369286911786745 90-91 23.202629099234244 26.959702382753665 26.55198058911246 23.285687928899634 92-93 23.226055367572428 26.825013110480956 26.581473987564806 23.367457534381806 94-95 23.21373784356771 26.799762527291726 26.665107305296704 23.321392323843856 96-97 23.452976083388112 26.436953961535924 26.684100970875953 23.42596898420001 98-99 23.104019008719575 26.60551890504781 26.5182339682207 23.77222811801191 100-101 23.247486604724653 26.712706269960172 26.40492395954152 23.634883165773648 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 107.0 1 88.0 2 170.0 3 312.5 4 270.0 5 251.0 6 537.5 7 933.5 8 1416.0 9 1715.5 10 1674.0 11 1726.0 12 1942.5 13 2264.0 14 2642.0 15 2967.0 16 3266.5 17 3537.5 18 3755.0 19 3934.0 20 4191.0 21 4740.0 22 5536.0 23 6865.0 24 8656.5 25 10976.5 26 15189.5 27 20341.0 28 24347.0 29 28703.5 30 33484.5 31 39893.5 32 49110.0 33 54932.5 34 58820.5 35 64483.5 36 71424.0 37 78011.5 38 83940.0 39 90044.5 40 95612.5 41 100830.5 42 104775.0 43 111146.5 44 117283.5 45 121910.5 46 126771.5 47 131211.0 48 136306.0 49 136189.0 50 133620.0 51 133490.5 52 135360.5 53 137115.0 54 136149.5 55 129952.5 56 119801.5 57 110536.0 58 104996.0 59 97652.5 60 84196.5 61 69772.0 62 56825.5 63 44709.0 64 33858.0 65 25403.0 66 18412.0 67 14353.5 68 11579.5 69 8832.5 70 6778.0 71 4991.0 72 3855.5 73 3432.0 74 2722.0 75 1991.0 76 1458.0 77 949.5 78 680.0 79 450.5 80 283.5 81 178.0 82 102.5 83 68.5 84 63.5 85 55.5 86 45.5 87 44.0 88 42.5 89 40.5 90 36.5 91 29.5 92 26.5 93 23.5 94 19.5 95 32.0 96 40.5 97 30.0 98 25.5 99 21.0 100 12.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04674931353418984 2 0.011180993088592585 3 0.009738284302967736 4 0.005188202748304747 5 0.007796176322318899 6 0.005437902345816741 7 0.005021736349963419 8 0.009044674309878865 9 0.0147322762532076 10-11 0.0022334241777461615 12-13 0.008545275114854879 14-15 0.008351064316789995 16-17 0.018477770215887496 18-19 0.011056143289836588 20-21 0.0034957943651679054 22-23 0.00993249510103262 24-25 0.02150190978575497 26-27 0.006908355531165145 28-29 0.017631566024319076 30-31 0.014219004858321836 32-33 0.012609829674355656 34-35 0.2631278869781937 36-37 0.4641083185756247 38-39 0.46082060720838347 40-41 0.48210056179635 42-43 0.4716409231005698 44-45 0.4871777869457606 46-47 0.49426648107512877 48-49 0.5017574690004886 50-51 0.512508423893366 52-53 0.5006199486118228 54-55 0.4874413587431343 56-57 0.4822947725944149 58-59 0.4802555592147336 60-61 0.477300780644175 62-63 0.47412404687582804 64-65 0.4906735813109285 66-67 0.46365053598018613 68-69 0.4906735813109285 70-71 0.4718212616987729 72-73 0.48039428121335137 74-75 0.4944329474734701 76-77 0.48160116260132607 78-79 0.48132371860409046 80-81 0.4835432705819749 82-83 0.4831825933855686 84-85 0.48344616518294237 86-87 0.4854160175633148 88-89 0.4992188564257833 90-91 0.5249379149695186 92-93 0.5121477466969598 94-95 0.4825444721919269 96-97 0.4546058616703072 98-99 0.44621318075393185 100-101 0.45516074966477826 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3604331.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.112767742452505 #Duplication Level Percentage of deduplicated Percentage of total 1 66.42035321150058 27.971449081675832 2 15.332439514030828 12.913829283591632 3 6.512177189795556 8.22737416474672 4 3.4841246212176276 5.86904523836393 5 2.13044262285963 4.485941768255446 6 1.3680903126096329 3.456844175137723 7 0.9410292405046687 2.774054209096072 8 0.6646460196212537 2.239206676420431 9 0.5013767897387702 1.9002927867952748 >10 2.408007165390178 17.718130443616975 >50 0.15078775259589147 4.426070622848537 >100 0.08216885589588431 6.129964676380576 >500 0.0030364908386368667 0.8677710954511801 >1k 0.0012542027310289885 0.8722745169418856 >5k 6.60106700541573E-5 0.1477512606777399 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5315 0.14746148453069377 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3692 0.10243232377936433 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 5.54887994471096E-5 12-13 0.0 0.0 0.0 0.0 2.635717973737706E-4 14-15 0.0 0.0 0.0 2.77443997235548E-5 3.1906059682088023E-4 16-17 0.0 0.0 0.0 8.323319917066441E-5 3.606771964062124E-4 18-19 0.0 0.0 0.0 1.942107980648836E-4 4.1616599585332203E-4 20-21 0.0 0.0 0.0 3.7454939626798984E-4 5.271435947475412E-4 22-23 2.77443997235548E-5 0.0 0.0 9.01692991015531E-4 6.381211936417605E-4 24-25 2.77443997235548E-5 0.0 0.0 0.0013039867870070756 6.381211936417605E-4 26-27 2.77443997235548E-5 0.0 0.0 0.0015536863845190688 6.658655933653152E-4 28-29 9.71053990324418E-5 0.0 0.0 0.0022056797780226067 6.658655933653152E-4 30-31 1.664663983413288E-4 0.0 0.0 0.005007864150101642 6.9360999308887E-4 32-33 1.664663983413288E-4 0.0 0.0 0.008836591311952205 7.352265926742023E-4 34-35 1.942107980648836E-4 0.0 0.0 0.013345056267029861 8.323319917066441E-4 36-37 2.0808299792666102E-4 0.0 0.0 0.022486835975941165 8.323319917066441E-4 38-39 2.496995975119932E-4 0.0 0.0 0.037330089828042985 8.323319917066441E-4 40-41 2.496995975119932E-4 0.0 0.0 0.05611304844088959 8.323319917066441E-4 42-43 2.7744399723554804E-4 0.0 0.0 0.07471566845553308 8.323319917066441E-4 44-45 3.051883969591028E-4 0.0 0.0 0.09591238984432895 8.739485912919763E-4 46-47 3.051883969591028E-4 0.0 0.0 0.12236667498073844 9.294373907390858E-4 48-49 3.606771964062124E-4 0.0 0.0 0.15280228147747807 9.71053990324418E-4 50-51 3.606771964062124E-4 0.0 0.0 0.18698338193689756 9.987983900479728E-4 52-53 3.606771964062124E-4 0.0 0.0 0.22056797780226067 0.0011097759889421922 54-55 3.606771964062124E-4 0.0 0.0 0.25789806763030365 0.0011513925885275242 56-57 4.1616599585332203E-4 0.0 0.0 0.30080478180278114 0.001206881387974634 58-59 4.1616599585332203E-4 0.0 0.0 0.35186834949398377 0.0012207535878364113 60-61 4.1616599585332203E-4 0.0 0.0 0.4091189183235391 0.0012207535878364113 62-63 4.1616599585332203E-4 0.0 0.0 0.4643302737734132 0.001276242387283521 64-65 4.1616599585332203E-4 0.0 0.0 0.5225380243934311 0.0013456033865924077 66-67 4.993991950239864E-4 0.0 0.0 0.5932584992887723 0.0013594755864541853 68-69 4.993991950239864E-4 0.0 0.0 0.6737033862872195 0.0013594755864541853 70-71 4.993991950239864E-4 0.0 0.0 0.764344340184073 0.0013594755864541853 72-73 4.993991950239864E-4 0.0 0.0 0.860561918425361 0.0014010921860395176 74-75 4.993991950239864E-4 0.0 0.0 0.9565852858685842 0.001456580985486627 76-77 4.993991950239864E-4 0.0 0.0 1.0653017161853335 0.0014704531853484044 78-79 5.132713948857639E-4 0.0 0.0 1.1913722685291668 0.0014981975850719594 80-81 5.826323941946508E-4 0.0 0.0 1.3341588217064415 0.001525941984795514 82-83 6.103767939182056E-4 0.0 0.0 1.4843670018097672 0.0016230473838279558 84-85 6.381211936417604E-4 0.0 0.0 1.6296366787623002 0.0016369195836897332 86-87 6.797377932270927E-4 0.0 0.0 1.7862260708020434 0.0016924083831368428 88-89 6.9360999308887E-4 0.0 0.0 1.9667311354034909 0.0017478971825839525 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3820 0.0 44.007267 1 GTATCAA 6990 0.0 39.69902 1 ATCAACG 8335 0.0 31.977734 3 TCAACGC 8450 0.0 31.597242 4 CAACGCA 8470 0.0 31.466742 5 TACATGG 12975 0.0 30.794127 2 GTACATG 13345 0.0 30.481335 1 AACGCAG 8950 0.0 29.989466 6 TATCAAC 9070 0.0 29.594337 2 ACATGGG 13345 0.0 28.735006 3 GAGTACA 9615 0.0 25.659477 1 AGTACAT 9270 0.0 24.768219 2 ACGCAGA 10980 0.0 24.402191 7 CATGGGG 7665 0.0 24.210331 4 CGCAGAG 11330 0.0 23.649029 8 GTGTAGC 2155 0.0 21.754408 1 CATGGGA 5420 0.0 21.049635 4 TATAGCG 115 0.0037983959 20.58254 5 GCAGAGT 12945 0.0 19.893793 9 TAACGCA 320 4.1654857E-10 19.23181 4 >>END_MODULE