FastQCFastQC Report
Thu 26 May 2016
SRR936367_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936367_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3604331
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57240.15880894401762768No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40810.11322489527182715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38350.045.2700651
GTATCAA73500.040.0577851
ATCAACG86000.033.6473353
CAACGCA87350.033.0730065
TCAACGC87600.033.0327724
AACGCAG91700.031.5041146
TATCAAC94750.030.7963712
TACATGG127350.030.4760042
GTACATG130050.030.31991
ACATGGG130850.028.7124823
TAGATCG909.0206455E-426.3540155
CATGGGG73550.025.2181594
ACGCAGA115150.024.9647927
GAGTACA97150.024.8716471
CGCAGAG117900.024.3020238
AGTACAT91350.023.8920232
GTGTAGC21850.021.5509991
GCAGAGT136600.020.8362649
GTGTACG5750.019.8530391
CATGGGA53750.019.5044214
Produced by FastQC (version 0.11.5)