##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936367_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3604331 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49745237049538 34.0 31.0 34.0 31.0 34.0 2 32.626018531594354 34.0 31.0 34.0 31.0 34.0 3 32.71400295921767 34.0 31.0 34.0 31.0 34.0 4 36.19015872848526 37.0 35.0 37.0 35.0 37.0 5 36.15298594940365 37.0 35.0 37.0 35.0 37.0 6 36.190082986274014 37.0 37.0 37.0 35.0 37.0 7 36.138546931455515 37.0 36.0 37.0 35.0 37.0 8 36.1162487573977 37.0 36.0 37.0 35.0 37.0 9 37.89241026975603 39.0 38.0 39.0 35.0 39.0 10-11 37.83132861549064 39.0 38.0 39.0 35.0 39.0 12-13 37.77537232290819 39.0 38.0 39.0 35.0 39.0 14-15 39.14785809072474 41.0 39.0 41.0 36.0 41.0 16-17 39.06942120465629 40.0 38.0 41.0 36.0 41.0 18-19 39.080293680019956 40.0 38.5 41.0 36.0 41.0 20-21 39.027148727461494 40.0 38.5 41.0 35.0 41.0 22-23 38.91224529600639 40.0 38.0 41.0 35.0 41.0 24-25 39.06403213245398 40.0 39.0 41.0 35.0 41.0 26-27 38.98614708249603 40.0 39.0 41.0 35.0 41.0 28-29 38.9007127813733 40.0 38.0 41.0 35.0 41.0 30-31 38.800193988842864 40.0 38.0 41.0 35.0 41.0 32-33 38.658312735428574 40.0 38.0 41.0 34.5 41.0 34-35 38.525802153020905 40.0 38.0 41.0 34.0 41.0 36-37 38.38248332353494 40.0 38.0 41.0 34.0 41.0 38-39 38.24662870862859 40.0 38.0 41.0 34.0 41.0 40-41 38.111005204571946 40.0 38.0 41.0 33.0 41.0 42-43 38.1438069921991 40.0 38.0 41.0 33.5 41.0 44-45 38.029658208416485 40.0 38.0 41.0 33.0 41.0 46-47 37.862584346443214 40.0 37.5 41.0 33.0 41.0 48-49 37.65362642887126 40.0 37.0 41.0 33.0 41.0 50-51 37.45994957177906 40.0 37.0 41.0 32.5 41.0 52-53 37.245298642105844 40.0 36.5 41.0 32.0 41.0 54-55 37.05012594570255 39.5 36.0 41.0 32.0 41.0 56-57 36.79494863263113 39.0 35.5 41.0 31.0 41.0 58-59 36.53113684619976 39.0 35.0 41.0 31.0 41.0 60-61 36.25036657288135 38.5 35.0 40.5 31.0 41.0 62-63 35.95241946425009 38.0 35.0 40.0 30.0 41.0 64-65 35.57916046001324 37.0 35.0 40.0 30.0 41.0 66-67 35.23549876523549 37.0 34.5 40.0 29.0 41.0 68-69 34.88590504035284 36.0 34.0 39.0 29.0 41.0 70-71 34.52062643525248 36.0 34.0 39.0 29.0 40.5 72-73 34.12583167306221 35.0 34.0 38.5 28.5 40.0 74-75 33.70563566442705 35.0 33.5 37.0 28.0 39.5 76-77 32.98766275905293 35.0 32.5 37.0 26.5 39.0 78-79 33.04952125651057 35.0 33.0 36.0 27.0 39.0 80-81 32.88914780579253 35.0 33.0 36.0 27.0 38.0 82-83 32.616473487035464 35.0 33.0 36.0 27.0 37.0 84-85 32.330716712754736 35.0 33.0 35.0 26.5 37.0 86-87 31.941838721249518 35.0 33.0 35.0 25.5 36.0 88-89 31.45448503480951 35.0 32.0 35.0 24.5 36.0 90-91 31.240103503257608 35.0 32.0 35.0 24.0 36.0 92-93 30.96156457328697 34.0 32.0 35.0 23.0 35.0 94-95 30.807748650165593 34.0 32.0 35.0 22.5 35.0 96-97 30.56425922036572 34.0 31.0 35.0 19.5 35.0 98-99 30.125699193553533 34.0 31.0 35.0 10.0 35.0 100-101 26.77967409208533 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1070003855460584 1101 2 0.12615056233911304 1101 3 0.1956601926403181 1101 4 0.12188112425539543 1101 5 0.11210643772800921 1101 6 0.15406637476402807 1101 7 0.12221770570238277 1101 8 0.11698716257992459 1101 9 0.2149061468812974 1101 10-11 0.1319558601632096 1101 12-13 0.23947066157074204 1101 14-15 0.3589553697800483 1101 16-17 0.3597999088897339 1101 18-19 0.36480952104250974 1101 20-21 0.3081103274926349 1101 22-23 0.31366117164260743 1101 24-25 0.21432792366311304 1101 26-27 0.33616138471831647 1101 28-29 0.31506370617513824 1101 30-31 0.25657361374659615 1101 32-33 0.31259021625275807 1101 34-35 0.3515307821235609 1101 36-37 0.33259763114167384 1101 38-39 0.3067259756869589 1101 40-41 0.2684692195364775 1101 42-43 0.23419247862374704 1101 44-45 0.3146090349238193 1101 46-47 0.2779948567906132 1101 48-49 0.25096365028800705 1101 50-51 0.26344764874525595 1101 52-53 0.23390138117314052 1101 54-55 0.24761670003346836 1101 56-57 0.24344272969441505 1101 58-59 0.08611918808834673 1101 60-61 0.12474814971447046 1101 62-63 0.05474774938372917 1101 64-65 0.18105953804010255 1101 66-67 0.20933202827894348 1101 68-69 0.3171382538924732 1101 70-71 0.23365950948686276 1101 72-73 0.2094015254485555 1101 74-75 0.10868604429047224 1101 76-77 -0.04569784560930401 1101 78-79 0.009977490585590942 1101 80-81 -0.02243241766377224 1101 82-83 0.07447547202618665 1101 84-85 0.2894931877672491 1101 86-87 0.1650266102148379 1101 88-89 0.185975610986425 1101 90-91 0.4049487060227719 1101 92-93 0.43707182139718626 1101 94-95 0.363445941601757 1101 96-97 0.18504736244966935 1101 98-99 0.2209647328909483 1101 100-101 0.42675385228476514 1102 1 0.0816828204480089 1102 2 0.17735505959297626 1102 3 0.14381401788358517 1102 4 0.14828976902090574 1102 5 0.10216696302092032 1102 6 0.17477596192733102 1102 7 0.12311837477083287 1102 8 0.1430218235247409 1102 9 0.1661771946078474 1102 10-11 0.19445466880200968 1102 12-13 0.24162775052212027 1102 14-15 0.20166595892992234 1102 16-17 0.24245870387035495 1102 18-19 0.3134505612189429 1102 20-21 0.23093063375741707 1102 22-23 0.26929778119852443 1102 24-25 0.2949624612865094 1102 26-27 0.2363496612268321 1102 28-29 0.2678276848822989 1102 30-31 0.22370996679726574 1102 32-33 0.20526908411567035 1102 34-35 0.23569742599897836 1102 36-37 0.12082375025459413 1102 38-39 0.1094099363600165 1102 40-41 0.05671149972308598 1102 42-43 0.020882363366276024 1102 44-45 -0.007479506211915066 1102 46-47 0.09571825048347904 1102 48-49 0.19307250879408144 1102 50-51 0.04866293911002373 1102 52-53 -0.055457049064479236 1102 54-55 -0.0954570988879695 1102 56-57 0.07013614086610431 1102 58-59 -0.0483784470078632 1102 60-61 0.013524636263902323 1102 62-63 0.15020499588485592 1102 64-65 0.19082155172645798 1102 66-67 0.09727694470961268 1102 68-69 0.015302024411852244 1102 70-71 0.037695198938983765 1102 72-73 -0.06057273286176468 1102 74-75 0.08542590818937157 1102 76-77 -0.12299045966711475 1102 78-79 0.0061169623091004155 1102 80-81 0.06164204593362399 1102 82-83 0.09700597708874881 1102 84-85 0.1363338636150644 1102 86-87 0.019090042843465937 1102 88-89 0.04829362279312477 1102 90-91 0.1649842148023808 1102 92-93 0.17623727183945803 1102 94-95 0.06991981909087386 1102 96-97 -0.008600009552555576 1102 98-99 0.012937631184751552 1102 100-101 0.4167498368795357 1103 1 0.03885458667757291 1103 2 0.08317773510967896 1103 3 0.11965899836673799 1103 4 0.07405290466748227 1103 5 0.09303451828866827 1103 6 0.10993776287349277 1103 7 0.16038835480429015 1103 8 0.16200802682372029 1103 9 0.14637434173069153 1103 10-11 0.16941644456515093 1103 12-13 0.1711008856290519 1103 14-15 0.25248125941739374 1103 16-17 0.20017280456648479 1103 18-19 0.1815809913090618 1103 20-21 0.1720563150482235 1103 22-23 0.2162407416431833 1103 24-25 0.14878083770080508 1103 26-27 0.25093468092355664 1103 28-29 0.21940747010706474 1103 30-31 0.24890243707230297 1103 32-33 0.1923106181217591 1103 34-35 0.20396096637276173 1103 36-37 0.20462476836838306 1103 38-39 0.25878980432900534 1103 40-41 0.29230680848498736 1103 42-43 0.33934826734699897 1103 44-45 0.3064476069138422 1103 46-47 0.3073869857559117 1103 48-49 0.3056015142444082 1103 50-51 0.26333969697522974 1103 52-53 0.1422764785379158 1103 54-55 0.18461008582953298 1103 56-57 0.21368388968340213 1103 58-59 0.25966932879124727 1103 60-61 0.22537549878256868 1103 62-63 0.25456525706881905 1103 64-65 0.19068979053349722 1103 66-67 0.20830800977176267 1103 68-69 0.24709649493872377 1103 70-71 0.14939406390400478 1103 72-73 0.12319051071206388 1103 74-75 0.3101844209517708 1103 76-77 0.18191033719580219 1103 78-79 0.1344294297060813 1103 80-81 0.08320950946320238 1103 82-83 0.2003872481120723 1103 84-85 0.15983412443927136 1103 86-87 0.11479633680134071 1103 88-89 0.19232704426705283 1103 90-91 0.36363557378642497 1103 92-93 0.47007012849407204 1103 94-95 0.36967698276255945 1103 96-97 0.42314499431483554 1103 98-99 0.3900844842058149 1103 100-101 0.5480365996014633 1104 1 -0.011573299856834751 1104 2 0.041236520710235425 1104 3 -0.010090702997260337 1104 4 0.003842083005444863 1104 5 0.023829725881505226 1104 6 -0.019335972900783815 1104 7 -0.03648116989781869 1104 8 0.04406739921780911 1104 9 -0.019312857348765533 1104 10-11 -0.03265785109984165 1104 12-13 0.05962029928389967 1104 14-15 0.006041473255876895 1104 16-17 0.12128460286936615 1104 18-19 0.14305643590020622 1104 20-21 0.07063753209896362 1104 22-23 0.13493148049131065 1104 24-25 0.05990008789174084 1104 26-27 0.19603848830711712 1104 28-29 0.14608098919661217 1104 30-31 0.06973877382356619 1104 32-33 0.025192256893795673 1104 34-35 0.04504109536986789 1104 36-37 0.09763607681562547 1104 38-39 0.21171068613737987 1104 40-41 0.35894852180514647 1104 42-43 0.26716812952849267 1104 44-45 0.1896492772284759 1104 46-47 0.1967313706924756 1104 48-49 0.166232151592844 1104 50-51 0.2708999273842849 1104 52-53 0.25570513170541176 1104 54-55 0.1342288406088059 1104 56-57 0.17732803351800186 1104 58-59 0.1784623178756206 1104 60-61 0.09515977417783716 1104 62-63 0.15028434477056862 1104 64-65 0.025031053864893238 1104 66-67 -0.03937486243639654 1104 68-69 0.19674717245619178 1104 70-71 0.2544345424234109 1104 72-73 0.14388200108480476 1104 74-75 0.2105966872761087 1104 76-77 0.13825182569215855 1104 78-79 0.07480301834333858 1104 80-81 0.12899353922396273 1104 82-83 0.15304572988613785 1104 84-85 0.2690749042508429 1104 86-87 0.3158365794336433 1104 88-89 0.3846683651081193 1104 90-91 0.45854650447314427 1104 92-93 0.4364211743170934 1104 94-95 0.3903174942281815 1104 96-97 0.4089903059855473 1104 98-99 0.2698854812674618 1104 100-101 0.25309827871799584 1105 1 -0.02537365282274351 1105 2 0.08016041503002214 1105 3 0.06602605610458312 1105 4 0.02735167427234586 1105 5 -0.0282050013670343 1105 6 -0.04407851312301858 1105 7 0.022235254377456215 1105 8 0.033007850844043674 1105 9 0.044538444425441526 1105 10-11 0.03473170058726538 1105 12-13 0.06530822931264169 1105 14-15 0.04250585410979113 1105 16-17 0.08827157775700556 1105 18-19 -0.005291302649652607 1105 20-21 0.047583959189964276 1105 22-23 -0.04007619727299527 1105 24-25 0.05375290033824598 1105 26-27 0.07786518499444384 1105 28-29 -0.07112354973051538 1105 30-31 -0.007766461186442086 1105 32-33 8.295911364797348E-4 1105 34-35 0.057375420201211114 1105 36-37 0.08929156053262233 1105 38-39 -0.016795587565454184 1105 40-41 0.11579863203539986 1105 42-43 0.13387284623424733 1105 44-45 0.0865046752812404 1105 46-47 0.09340499984654826 1105 48-49 0.04207587240220079 1105 50-51 0.03792252371828653 1105 52-53 0.1215643789211569 1105 54-55 0.09022039323723874 1105 56-57 0.08310437871816845 1105 58-59 0.015751691051768546 1105 60-61 0.08942870220232635 1105 62-63 0.14442783723274744 1105 64-65 0.033592171826242634 1105 66-67 0.08167280242005859 1105 68-69 0.2061343249065004 1105 70-71 0.13883797286070632 1105 72-73 0.11481935118531794 1105 74-75 0.054405517470250686 1105 76-77 -0.02725593906020407 1105 78-79 -0.011149588232996166 1105 80-81 0.021284716475214793 1105 82-83 0.05687162720342798 1105 84-85 0.058918416814627506 1105 86-87 0.08747320462740049 1105 88-89 0.1849252826775185 1105 90-91 0.20638877338464567 1105 92-93 0.2866078770771523 1105 94-95 0.17118729408616673 1105 96-97 0.15831634803256378 1105 98-99 0.16704789600343517 1105 100-101 0.2830055531074436 1106 1 -0.07474453628360322 1106 2 -0.01211571304209258 1106 3 -0.010059060431686362 1106 4 -0.0018611656127873744 1106 5 0.02082275693026503 1106 6 0.016508022219227314 1106 7 0.09413175399922125 1106 8 0.054964099564379865 1106 9 -0.025615649314815414 1106 10-11 0.0675585951513753 1106 12-13 0.011631711702555947 1106 14-15 0.09550888446655392 1106 16-17 0.04868526973181275 1106 18-19 0.14243769421215546 1106 20-21 0.11038952285118597 1106 22-23 0.0776142112188083 1106 24-25 0.10818682800987034 1106 26-27 0.08422240489065302 1106 28-29 0.052830348434660834 1106 30-31 0.0729264384782411 1106 32-33 0.08420721457510183 1106 34-35 0.14354278288084288 1106 36-37 0.06163312869074389 1106 38-39 0.1199984106908829 1106 40-41 0.008897320063063319 1106 42-43 0.13914323441619558 1106 44-45 0.15075940760509354 1106 46-47 0.11311158887073702 1106 48-49 0.04233337301437956 1106 50-51 0.10910764990970989 1106 52-53 0.10425877038765208 1106 54-55 0.0767133889518945 1106 56-57 0.08418467215602021 1106 58-59 0.21891756927395534 1106 60-61 0.06856882391458186 1106 62-63 0.07717243907948301 1106 64-65 0.16018130886511983 1106 66-67 0.0842835899312675 1106 68-69 0.09615961997533162 1106 70-71 0.04946519777795544 1106 72-73 0.010970966470978283 1106 74-75 0.1572444997848379 1106 76-77 -0.025774213363646936 1106 78-79 -0.009950575817263996 1106 80-81 0.059036545356512704 1106 82-83 0.09746783023822303 1106 84-85 0.13445230290997046 1106 86-87 0.10778462900255903 1106 88-89 0.22028056455694411 1106 90-91 0.44326994567431655 1106 92-93 0.2925132841600977 1106 94-95 0.25833029299830557 1106 96-97 0.29470860055118564 1106 98-99 0.3178179079210146 1106 100-101 0.5277969202897026 1107 1 -1.0666990387164788E-4 1107 2 0.08872863600660708 1107 3 0.07828506073132502 1107 4 0.0575463092611912 1107 5 0.04051840582035737 1107 6 0.04710702487981422 1107 7 0.09442772635677699 1107 8 0.06566933230357108 1107 9 0.0706272690210028 1107 10-11 0.09052556642215137 1107 12-13 0.0763815713435676 1107 14-15 0.16056708740276804 1107 16-17 0.13154432177941544 1107 18-19 0.18292535471344706 1107 20-21 0.1026225273222181 1107 22-23 0.0804115313574627 1107 24-25 0.16999045118775769 1107 26-27 0.2276932844475752 1107 28-29 0.18673784209757827 1107 30-31 0.0992721639565346 1107 32-33 0.10413977157163856 1107 34-35 0.15723843400328974 1107 36-37 0.22141485698328012 1107 38-39 0.21872779447680557 1107 40-41 0.1199330893735393 1107 42-43 -0.019131099776217297 1107 44-45 -0.006051621392749951 1107 46-47 0.0684099033351373 1107 48-49 0.023666930326790236 1107 50-51 0.09200720050051103 1107 52-53 0.006999016786963352 1107 54-55 0.055388775920398814 1107 56-57 0.040717755019720414 1107 58-59 -0.05065362612323554 1107 60-61 1.1177562402764352E-4 1107 62-63 0.07226731579982726 1107 64-65 0.07588332143994592 1107 66-67 -0.0689342862782425 1107 68-69 -0.004729748124610467 1107 70-71 0.10139811492690143 1107 72-73 0.039998581008191536 1107 74-75 0.06223467558609741 1107 76-77 -0.03875365230721428 1107 78-79 -0.03175207454240336 1107 80-81 0.14848791194516053 1107 82-83 0.20299983450498615 1107 84-85 0.14653238398891943 1107 86-87 0.2452256531152841 1107 88-89 0.27863911413984965 1107 90-91 0.12291549319713369 1107 92-93 0.33887779114009575 1107 94-95 0.267996553005279 1107 96-97 0.08141931147641657 1107 98-99 0.16014082027412968 1107 100-101 0.3225757149027437 1108 1 -0.15428757564890816 1108 2 -0.1491335426286966 1108 3 -0.08162448189131055 1108 4 -0.07035618777687347 1108 5 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-0.7178195707128836 2316 74-75 -0.6542247726722437 2316 76-77 -0.5559176440517888 2316 78-79 -0.7386539163346484 2316 80-81 -0.8371738600399183 2316 82-83 -0.8943750158711907 2316 84-85 -0.7553533172203188 2316 86-87 -0.8822261605065407 2316 88-89 -0.798383260437781 2316 90-91 -0.9057021369501612 2316 92-93 -0.8559816483364564 2316 94-95 -0.8508772212646925 2316 96-97 -0.810229440290211 2316 98-99 -1.0268114036809557 2316 100-101 -0.9669163864008787 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 17.0 4 30.0 5 59.0 6 139.0 7 256.0 8 556.0 9 986.0 10 1751.0 11 2793.0 12 4184.0 13 6332.0 14 8080.0 15 10440.0 16 12598.0 17 16304.0 18 15413.0 19 10859.0 20 9965.0 21 10550.0 22 11629.0 23 13501.0 24 16217.0 25 19850.0 26 24724.0 27 31341.0 28 39905.0 29 49864.0 30 62458.0 31 78787.0 32 98919.0 33 128570.0 34 179363.0 35 269881.0 36 467247.0 37 866220.0 38 943392.0 39 190943.0 40 204.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.584431227340765 17.945375548321106 11.731241221785577 24.73895200255255 2 16.78056637110186 19.761827656663083 37.71729951834397 25.740306453891087 3 20.629625858446406 22.657963433436052 28.246379147753075 28.466031560364463 4 13.034318990126046 14.770480291626933 35.67538608413045 36.519814634116564 5 14.11868110892146 37.17092020682895 33.85030398151557 14.860094702734017 6 34.968514267973724 34.636469292082225 16.254028833644856 14.140987606299198 7 30.788570750022682 31.064627527272055 19.440001487099824 18.706800235605442 8 27.986747055140054 34.44969399314325 18.881700931462735 18.681858020253966 9 26.76455075851802 14.998456024155384 19.118998782298295 39.1179944350283 10-11 26.302700648111156 25.69970202423758 26.603728303813202 21.39386902383806 12-13 26.13175349257053 22.563170209604866 27.54569192411515 23.75938437370945 14-15 22.81297139469155 24.35164805896018 25.023867119862185 27.81151342648608 16-17 23.3251172484513 27.558837858653746 25.73056938467036 23.385475508224594 18-19 23.21677726046803 26.487384205279703 27.344561306938793 22.951277227313476 20-21 23.77534468606583 25.731366754519318 27.503873465543737 22.98941509387111 22-23 23.23045328573129 25.716035198804104 27.41189964173617 23.641611873728436 24-25 23.10176338897408 25.807733252909827 27.678120820042324 23.41238253807377 26-27 23.075563570784492 26.15549698272872 27.40449469376431 23.36444475272248 28-29 22.97832381749851 26.373352277606294 27.486328313850116 23.161995591045084 30-31 23.126342755843066 26.10036444025315 27.431970389766985 23.341322414136805 32-33 22.810969225922435 26.03453640647302 27.587854839942526 23.566639527662016 34-35 23.32549218282565 26.187065888831196 27.390761333310955 23.096680595032208 36-37 23.350462735227556 26.030503874646293 27.36202771722559 23.25700567290056 38-39 23.080377257563683 26.34055502162955 27.34760820879787 23.231459512008897 40-41 23.36875579862298 26.19193608530246 27.26391835982758 23.175389756246975 42-43 23.202714162663586 26.139186569689215 27.225376022538118 23.432723245109084 44-45 23.19503999030017 26.283850738578597 27.36340086729696 23.157708403824277 46-47 23.289022905891457 26.567193542358602 26.908134054801604 23.235649496948337 48-49 23.439627084386615 26.507748171522532 26.835428426185786 23.217196317905067 50-51 23.339659418027956 26.63682585925849 26.817285748968196 23.20622897374536 52-53 23.569343538595124 26.541509732627265 26.71209377905121 23.177052949726402 54-55 23.387464277197708 26.535190939860843 26.875949122655886 23.201395660285566 56-57 23.191885410007814 26.761932665672276 26.850009877299524 23.196172047020386 58-59 23.294972479955785 26.48885409077314 26.885007887840075 23.331165541430998 60-61 23.130476809844275 26.59632082674858 27.06305655929826 23.210145804108883 62-63 23.25774186665986 26.577733842979463 27.05848880138922 23.106035488971465 64-65 23.030113610116583 26.539213364701418 27.021817409508447 23.408855615673556 66-67 23.03965701263286 26.743298548329776 26.910125623867508 23.306918815169862 68-69 23.30698817616917 26.48183532533499 27.078395408190868 23.13278109030497 70-71 23.007646233752325 26.396534606286032 26.998936138778884 23.596883021182755 72-73 23.240190030316842 26.567634972658794 26.876810180821785 23.315364816202575 74-75 23.04324991239706 26.75747593658851 26.756990409593346 23.442283741421086 76-77 23.15522852152816 26.62193314099344 26.81287389320939 23.40996444426901 78-79 23.211220759137614 26.74423125846384 26.764797206697082 23.279750775701462 80-81 23.16519596758867 26.648110101975558 26.732120983437813 23.454572946997956 82-83 23.26865928795108 26.876110989806428 26.646775781691527 23.208453940550967 84-85 23.247395100342963 26.80012435100332 26.529504162165697 23.422976386488024 86-87 23.075188012740714 26.63420422835782 26.761455801328655 23.529151957572807 88-89 23.208427433111538 26.71945324104404 26.626284867499837 23.445834458344585 90-91 23.02903776173915 26.8908873857721 26.6412074694969 23.438867382991848 92-93 23.067152900183693 26.774467815133875 26.65254769639428 23.505831588288157 94-95 23.00187695342575 26.733560010487633 26.715803170983104 23.54875986510351 96-97 23.223980645018088 26.414930748229864 26.73877155794287 23.62231704880918 98-99 22.826979927779405 26.569618795666766 26.602385804477237 24.0010154720766 100-101 22.788402354354194 26.69151753578815 26.65567146801439 23.864408641843266 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 87.0 1 65.5 2 131.0 3 251.0 4 204.5 5 182.5 6 435.0 7 783.0 8 1188.0 9 1359.0 10 1170.5 11 1121.0 12 1267.0 13 1421.0 14 1608.0 15 1801.0 16 1934.0 17 2180.0 18 2481.0 19 2772.5 20 3243.5 21 4037.5 22 5201.0 23 6894.0 24 8896.0 25 11312.5 26 15616.5 27 21142.0 28 25307.0 29 29279.5 30 34158.0 31 40972.5 32 50292.0 33 55852.0 34 59656.5 35 65260.5 36 72518.0 37 79343.0 38 84770.0 39 90617.5 40 95951.5 41 101074.5 42 105226.5 43 111878.5 44 117991.5 45 122912.5 46 127659.0 47 131010.0 48 136048.5 49 136568.0 50 134420.0 51 134405.5 52 135698.5 53 136892.5 54 136068.5 55 130100.0 56 120233.5 57 111090.0 58 105639.0 59 98291.5 60 84096.0 61 69029.0 62 55995.5 63 44016.0 64 33225.0 65 24852.5 66 17975.0 67 13827.0 68 11120.5 69 8515.5 70 6301.0 71 4549.0 72 3614.0 73 3326.5 74 2570.0 75 1783.0 76 1296.0 77 854.5 78 634.5 79 393.5 80 203.5 81 105.5 82 49.0 83 28.0 84 19.5 85 9.0 86 4.0 87 2.5 88 2.5 89 2.5 90 1.0 91 0.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2634053309754293 2 0.020031456600406564 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.051883969591028E-4 12-13 4.1616599585332206E-5 14-15 0.0 16-17 5.271435947475412E-4 18-19 0.0 20-21 1.3872199861777402E-5 22-23 1.1097759889421922E-4 24-25 0.0021640631784372744 26-27 0.0022472963776079386 28-29 0.0020947021791283874 30-31 0.0010126705899097502 32-33 0.001317858986868853 34-35 0.0015398141846572912 36-37 5.548879944710961E-5 38-39 8.323319917066441E-5 40-41 7.490987925359796E-4 42-43 0.0034819221653061274 44-45 0.0040090657600536685 46-47 0.013220206468273862 48-49 0.014232877058183612 50-51 0.005715346343052289 52-53 0.006728016932962039 54-55 4.300381957150994E-4 56-57 0.0029686507704203636 58-59 0.0013594755864541853 60-61 0.0011930091881128565 62-63 0.0 64-65 8.462041915684214E-4 66-67 0.0 68-69 0.0 70-71 2.0808299792666102E-4 72-73 0.0017756415823075074 74-75 0.0 76-77 0.19088147009805703 78-79 0.1031814225719003 80-81 9.987983900479728E-4 82-83 0.0 84-85 4.8552699516220905E-4 86-87 0.12139562099041402 88-89 0.5835618315853899 90-91 0.3088090411230267 92-93 0.2936605988739658 94-95 0.002386018376225713 96-97 0.002524740374843487 98-99 0.002663462373461261 100-101 8.462041915684214E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3604331.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.83433711721872 #Duplication Level Percentage of deduplicated Percentage of total 1 63.890519547769784 25.450364942601144 2 16.09879981170494 12.82570037764144 3 7.079172743242244 8.459824606960128 4 3.8586812522211362 6.148320393154736 5 2.26768208856147 4.516580639521812 6 1.536103729128922 3.671380427588299 7 1.0516731842440399 2.9324892910821174 8 0.7335703760337323 2.337703172650608 9 0.5580331565765775 2.000599279349138 >10 2.6563798792193443 18.410428271739853 >50 0.17245722002164907 4.750607254225501 >100 0.09198353468615894 6.474325166943681 >500 0.0034813215473644297 0.9378667076070576 >1k 0.0013925286120269457 0.9250530290803062 >5k 6.962643060134728E-5 0.15875643985415624 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5724 0.15880894401762768 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4081 0.11322489527182715 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.54887994471096E-5 2 2.77443997235548E-5 0.0 0.0 0.0 5.54887994471096E-5 3 2.77443997235548E-5 0.0 0.0 0.0 5.54887994471096E-5 4 2.77443997235548E-5 0.0 0.0 0.0 5.54887994471096E-5 5 2.77443997235548E-5 0.0 0.0 0.0 8.32331991706644E-5 6 2.77443997235548E-5 0.0 0.0 0.0 1.109775988942192E-4 7 2.77443997235548E-5 0.0 0.0 0.0 1.109775988942192E-4 8 2.77443997235548E-5 0.0 0.0 0.0 1.3872199861777402E-4 9 2.77443997235548E-5 0.0 0.0 0.0 1.3872199861777402E-4 10-11 5.54887994471096E-5 0.0 0.0 0.0 1.664663983413288E-4 12-13 5.54887994471096E-5 0.0 0.0 0.0 2.635717973737706E-4 14-15 1.3872199861777402E-4 0.0 0.0 5.54887994471096E-5 3.1906059682088023E-4 16-17 1.3872199861777402E-4 0.0 0.0 9.710539903244181E-5 3.884215961297672E-4 18-19 1.3872199861777402E-4 0.0 0.0 2.219551977884384E-4 5.271435947475412E-4 20-21 1.3872199861777402E-4 0.0 0.0 4.0229379599154467E-4 5.965045940564283E-4 22-23 1.664663983413288E-4 0.0 0.0 9.433095906008632E-4 6.381211936417605E-4 24-25 2.219551977884384E-4 0.0 0.0 0.00138721998617774 6.658655933653152E-4 26-27 2.219551977884384E-4 0.0 0.0 0.0016369195836897334 6.9360999308887E-4 28-29 3.051883969591028E-4 0.0 0.0 0.0022889129771932713 6.9360999308887E-4 30-31 3.051883969591028E-4 0.0 0.0 0.005118841748995861 7.074821929506474E-4 32-33 3.051883969591028E-4 0.0 0.0 0.008975313310569978 7.213543928124248E-4 34-35 3.3293279668265765E-4 0.0 0.0 0.01355313926495652 7.907153921213118E-4 36-37 4.439103955768768E-4 0.0 0.0 0.022819768772623823 8.045875919830892E-4 38-39 4.7165479530043164E-4 0.0 0.0 0.037884977822514085 8.323319917066441E-4 40-41 4.8552699516220905E-4 0.0 0.0 0.05688989163314912 8.600763914301988E-4 42-43 4.993991950239864E-4 0.0 0.0 0.07565897804613395 8.600763914301988E-4 44-45 5.271435947475412E-4 0.0 0.0 0.0970499102329947 8.600763914301988E-4 46-47 5.271435947475412E-4 0.0 0.0 0.12383712816608686 8.600763914301988E-4 48-49 5.548879944710961E-4 0.0 0.0 0.1545640508599238 8.878207911537536E-4 50-51 5.548879944710961E-4 0.0 0.0 0.1889393621174082 9.433095906008633E-4 52-53 5.548879944710961E-4 0.0 0.0 0.2228014019800068 9.433095906008633E-4 54-55 5.548879944710961E-4 0.0 0.0 0.26046442460473246 9.571817904626406E-4 56-57 5.826323941946508E-4 0.0 0.0 0.3037318159736162 9.71053990324418E-4 58-59 5.826323941946508E-4 0.0 0.0 0.35523929406039567 9.71053990324418E-4 60-61 5.826323941946508E-4 0.0 0.0 0.41271181808773943 9.71053990324418E-4 62-63 5.826323941946508E-4 0.0 0.0 0.46833933953346685 0.0010265427897715276 64-65 5.826323941946508E-4 0.0 0.0 0.5270742337482324 0.0010265427897715276 66-67 5.826323941946508E-4 0.0 0.0 0.5984744464368006 0.0010265427897715276 68-69 5.826323941946508E-4 0.0 0.0 0.6795019658294423 0.0010265427897715276 70-71 5.826323941946508E-4 0.0 0.0 0.7705035969227022 0.0010265427897715276 72-73 5.965045940564283E-4 0.0 0.0 0.8674980183562497 0.0011236481888039695 74-75 6.103767939182056E-4 0.0 0.0 0.9642566123921471 0.0012207535878364113 76-77 6.103767939182056E-4 0.0 0.0 1.073194997906685 0.0012207535878364113 78-79 6.24248993779983E-4 0.0 0.0 1.1993626556495505 0.001276242387283521 80-81 6.9360999308887E-4 0.0 0.0 1.3428150744201905 0.0013039867870070756 82-83 6.9360999308887E-4 0.0 0.0 1.49305099892324 0.0014149643859012948 84-85 7.074821929506474E-4 0.0 0.0 1.6388616916703822 0.0014149643859012948 86-87 7.213543928124248E-4 0.0 0.0 1.7965192430994823 0.0014981975850719594 88-89 7.213543928124248E-4 0.0 0.0 1.9778982562922218 0.001595302984104401 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3835 0.0 45.270065 1 GTATCAA 7350 0.0 40.057785 1 ATCAACG 8600 0.0 33.647335 3 CAACGCA 8735 0.0 33.073006 5 TCAACGC 8760 0.0 33.032772 4 AACGCAG 9170 0.0 31.504114 6 TATCAAC 9475 0.0 30.796371 2 TACATGG 12735 0.0 30.476004 2 GTACATG 13005 0.0 30.3199 1 ACATGGG 13085 0.0 28.712482 3 TAGATCG 90 9.0206455E-4 26.354015 5 CATGGGG 7355 0.0 25.218159 4 ACGCAGA 11515 0.0 24.964792 7 GAGTACA 9715 0.0 24.871647 1 CGCAGAG 11790 0.0 24.302023 8 AGTACAT 9135 0.0 23.892023 2 GTGTAGC 2185 0.0 21.550999 1 GCAGAGT 13660 0.0 20.836264 9 GTGTACG 575 0.0 19.853039 1 CATGGGA 5375 0.0 19.504421 4 >>END_MODULE