FastQCFastQC Report
Thu 26 May 2016
SRR936366_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936366_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345185
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39530.1685581308084437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29050.12387082468973663No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27630.11781586527288893No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24050.1025505450529489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23800.053.310981
GTATCAA44050.043.9578321
TCAACGC55000.034.76994
ATCAACG55850.034.2414553
CAACGCA58000.033.053075
AACGCAG59950.032.0562256
TATCAAC59850.031.9509262
TACATGG88600.030.932222
GTACATG90850.030.6415881
ACATGGG88000.029.9617083
CATGGGG62100.025.3826754
ACGCAGA75450.025.3458527
GAGTACA59750.025.0384831
CGCAGAG78000.024.5182828
TGGTATC13250.022.5056172
GTGGTAT14900.022.2418371
AGTACAT62250.022.1268652
GCAGAGT94350.020.2694879
CGCGAAA600.004728098419.81874872-73
TAAGGTG8450.019.6063215
Produced by FastQC (version 0.11.5)