FastQCFastQC Report
Thu 26 May 2016
SRR936366_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936366_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345185
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42580.1815635013868842No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32030.1365777113532621No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28210.12028901771075629No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24660.10515161916863701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24950.053.3951031
GTATCAA46000.043.751861
TCAACGC58300.033.8493084
ATCAACG58350.033.7390023
CAACGCA60250.032.6750345
AACGCAG62750.031.6000356
TATCAAC62850.031.4789752
TACATGG85450.028.2615222
GTACATG87400.027.7634011
ACATGGG85750.027.2179853
TACCGTA1257.470773E-626.5651977
ACGCAGA76550.025.9033587
CGCAGAG79600.024.7320448
CATGGGG58650.023.6987064
GAGTACA60850.022.5188351
GTGGTAT15850.021.9132881
TGGTATC14300.021.5658782
AGTACAT63050.020.3926662
TATACGA1404.891925E-420.3335422
GCAGAGT96900.020.218619
Produced by FastQC (version 0.11.5)