FastQCFastQC Report
Thu 26 May 2016
SRR936365_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936365_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894685
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52030.5815454601340136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37350.4174653649049665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28160.3147476486137579No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17520.1958231109273096No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT17500.19559956856323732No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA16920.18911684000514148No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG16130.18028691662428678No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT15490.1731335609739741No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG14880.1663155188697698No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA14400.1609505021320353No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC13300.14865567210806038No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA13090.14630847728530152No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG13060.1459731637391931No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA12980.14507899428290405No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT12500.1397139775451695No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC12420.13881980808888045No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT12260.13703146917630227No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT11080.12384246969603828No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG11030.12328361378585759No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA10920.1220541307834601No Hit
GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG10650.11903630886848444No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC10510.11747151231997854No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG10410.11635380049961719No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10130.11322420740260539No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10080.11266535149242471No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG9990.11165941085409949No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG9900.11065347021577426No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA9890.11054169903373812No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC9840.10998284312355745No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT9670.10808273302894315No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT9470.10584730938822044No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG9090.10160000447084729No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGCA250.00509735556.7828069
GGTATCA18900.042.8315851
CATTCCG1650.040.149469
GTATCAA32900.040.0017281
TCAGGCG500.001645757737.8573233
ACAACGC651.3576649E-436.4012643
TACCGCG1750.035.151269
GTTCCGT550.002622304334.4292141
GTACATG47100.034.2738841
ATGGGTA5650.033.5020565
CATGGGG13300.033.4453774
CATGGGT7050.032.8902244
TCAACGC39950.032.4559024
ATCAACG40400.032.328653
CAACGCA40600.031.9362875
CAGGCGT753.1465667E-431.5477684
GATACCG1950.031.5460057
ACATGGG48050.031.514943
AACGCAG42350.030.7266246
AGTACAT29750.030.3812942
Produced by FastQC (version 0.11.5)