##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936365_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 894685 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05961986620989 33.0 31.0 34.0 30.0 34.0 2 32.14640236507821 34.0 31.0 34.0 30.0 34.0 3 32.20885674846454 34.0 31.0 34.0 30.0 34.0 4 35.72185070723216 37.0 35.0 37.0 35.0 37.0 5 35.7547181410217 37.0 35.0 37.0 35.0 37.0 6 35.86181505222508 37.0 35.0 37.0 35.0 37.0 7 35.81842995020594 37.0 35.0 37.0 35.0 37.0 8 35.83319715877655 37.0 35.0 37.0 35.0 37.0 9 37.55582914657114 39.0 38.0 39.0 35.0 39.0 10-11 37.49110413162174 39.0 37.5 39.0 35.0 39.0 12-13 37.456837881489015 39.0 37.0 39.0 35.0 39.0 14-15 38.73768924258259 40.0 38.0 41.0 35.0 41.0 16-17 38.71757769494292 40.0 38.0 41.0 35.0 41.0 18-19 38.76348435482879 40.0 38.5 41.0 35.0 41.0 20-21 38.71348128112129 40.0 38.5 41.0 35.0 41.0 22-23 38.57306482169702 40.0 38.0 41.0 34.0 41.0 24-25 38.724484595136836 40.0 38.5 41.0 35.0 41.0 26-27 38.66972230449824 40.0 38.0 41.0 35.0 41.0 28-29 38.56972901076915 40.0 38.0 41.0 34.5 41.0 30-31 38.41818293589364 40.0 38.0 41.0 34.0 41.0 32-33 38.219194465091064 40.0 38.0 41.0 34.0 41.0 34-35 37.96891028686074 40.0 38.0 41.0 33.5 41.0 36-37 37.780512135556094 40.0 38.0 41.0 33.0 41.0 38-39 37.63485248998251 40.0 38.0 41.0 33.0 41.0 40-41 37.492190547511136 40.0 38.0 41.0 33.0 41.0 42-43 37.330531416084995 40.0 37.0 41.0 33.0 41.0 44-45 37.143553876504015 40.0 37.0 41.0 32.0 41.0 46-47 36.86611265417437 40.0 37.0 41.0 31.5 41.0 48-49 36.692175458401564 40.0 37.0 41.0 31.0 41.0 50-51 36.384126815583144 39.5 37.0 40.5 30.5 41.0 52-53 36.45962042506581 39.5 37.0 40.5 31.0 41.0 54-55 36.63266512795006 40.0 37.0 41.0 31.0 41.0 56-57 36.55947791680871 40.0 37.0 41.0 31.0 41.0 58-59 36.42825799024238 40.0 37.0 41.0 30.5 41.0 60-61 36.25411290007098 40.0 36.0 41.0 30.0 41.0 62-63 36.00865388376915 39.0 36.0 41.0 29.0 41.0 64-65 35.76497873553262 39.0 35.5 41.0 29.0 41.0 66-67 35.47416856211963 39.0 35.0 40.5 28.5 41.0 68-69 35.1262338141357 38.0 35.0 40.0 28.0 41.0 70-71 34.754789115722296 37.5 35.0 40.0 28.0 41.0 72-73 34.35966513353862 37.0 35.0 39.5 27.0 41.0 74-75 33.92009981166556 36.5 34.0 39.0 26.0 41.0 76-77 33.49581081609729 36.0 34.0 39.0 26.0 40.0 78-79 33.06658153428302 35.5 34.0 37.5 26.0 39.5 80-81 32.61916652229556 35.0 34.0 37.0 25.0 39.0 82-83 32.20621615428894 35.0 33.0 37.0 24.0 39.0 84-85 31.79000206776687 35.0 33.0 36.0 22.5 37.5 86-87 31.445119790764345 35.0 33.0 36.0 20.5 37.0 88-89 31.087781733235722 35.0 32.5 35.5 18.5 37.0 90-91 30.72412692735432 35.0 32.0 35.0 13.5 36.0 92-93 30.42470701978909 35.0 32.0 35.0 4.5 36.0 94-95 30.076673913164967 34.5 31.5 35.0 2.0 36.0 96-97 29.730044093731316 34.0 31.0 35.0 2.0 35.5 98-99 29.361394233724717 34.0 31.0 35.0 2.0 35.0 100-101 26.300664479677202 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17578984071094084 1101 2 0.14396550806353048 1101 3 0.1851385349128094 1101 4 0.04044109726200418 1101 5 0.20796542703799759 1101 6 0.036472652077414125 1101 7 0.23831563897955732 1101 8 0.1380002154425597 1101 9 0.19645685630262477 1101 10-11 0.3029595848079225 1101 12-13 0.13039101722051782 1101 14-15 0.2789866346369436 1101 16-17 0.3668922930326701 1101 18-19 0.4110518303406536 1101 20-21 0.6085637177963292 1101 22-23 0.4427107759076634 1101 24-25 0.4658768406762377 1101 26-27 0.38346861387143605 1101 28-29 0.38600743088274925 1101 30-31 0.3990917315932876 1101 32-33 0.3715157242084288 1101 34-35 0.28009894141373337 1101 36-37 0.20485677859078777 1101 38-39 0.14815510552321598 1101 40-41 0.31047423232901394 1101 42-43 0.3297244264887098 1101 44-45 0.18734085199714912 1101 46-47 -0.023888509146374304 1101 48-49 0.07678924064063608 1101 50-51 0.166250117345335 1101 52-53 0.18422577129223328 1101 54-55 0.20012474661844237 1101 56-57 0.31225609108457775 1101 58-59 0.093242792438609 1101 60-61 0.06440524221540755 1101 62-63 0.002442472035554033 1101 64-65 0.14801468837984544 1101 66-67 0.4703683809886883 1101 68-69 0.4659250328106168 1101 70-71 0.4969380131174006 1101 72-73 0.4146556779095434 1101 74-75 0.15762331181537803 1101 76-77 0.06071953748257641 1101 78-79 0.266761238444424 1101 80-81 0.100768335348981 1101 82-83 0.11507970998199113 1101 84-85 0.05925136947949028 1101 86-87 0.07859433244100344 1101 88-89 -0.019755551925687342 1101 90-91 -0.03502223965057638 1101 92-93 0.09761009343923277 1101 94-95 0.2060921462369798 1101 96-97 0.3706161003331552 1101 98-99 0.4351133884861689 1101 100-101 0.8670234367776892 1102 1 0.1645259177635623 1102 2 0.23485244043568088 1102 3 0.16467881913298044 1102 4 0.0940647566705195 1102 5 0.20173149197728435 1102 6 0.27679675761114453 1102 7 0.014942814209270239 1102 8 0.08060960475328471 1102 9 0.08016146823278092 1102 10-11 0.18513525768882744 1102 12-13 0.26070167990849313 1102 14-15 0.2773801407083809 1102 16-17 0.29159019831230637 1102 18-19 0.3334267823304913 1102 20-21 0.20333578031294763 1102 22-23 0.1504175150573701 1102 24-25 0.2507322192917627 1102 26-27 0.250897780414995 1102 28-29 0.08724387724432603 1102 30-31 0.11039923299666299 1102 32-33 -0.05351086603591426 1102 34-35 0.04685079534466041 1102 36-37 0.21485397184277844 1102 38-39 0.19846975674784062 1102 40-41 0.15448551841050318 1102 42-43 0.32801083296491385 1102 44-45 0.2709967151460262 1102 46-47 0.3367527589128372 1102 48-49 0.4559587386759034 1102 50-51 0.26033157212714997 1102 52-53 0.32440067601052647 1102 54-55 0.37905862554419656 1102 56-57 0.4643264370532023 1102 58-59 0.3893879456279663 1102 60-61 0.3182238967490463 1102 62-63 0.34606967680998224 1102 64-65 0.1696524997072828 1102 66-67 0.2615093979178127 1102 68-69 0.30047462361629584 1102 70-71 0.28608476172141906 1102 72-73 0.18118963956049328 1102 74-75 0.12886891703887926 1102 76-77 0.04100951886940862 1102 78-79 0.1900373042700494 1102 80-81 0.19655230438611682 1102 82-83 0.3969769239152754 1102 84-85 0.5725021745729926 1102 86-87 0.5780846860912554 1102 88-89 0.2879231197438834 1102 90-91 0.2887435298415504 1102 92-93 0.40062439308173836 1102 94-95 0.4013347083547494 1102 96-97 0.300543420331973 1102 98-99 0.49758199722549534 1102 100-101 0.47920841624410926 1103 1 0.28206822624798633 1103 2 0.3207317396381555 1103 3 0.334329982749459 1103 4 0.29272111164278414 1103 5 0.2879108387612703 1103 6 0.3245487088081376 1103 7 0.2798493200930423 1103 8 0.3357139644979057 1103 9 0.1975812720833332 1103 10-11 0.39255424484181844 1103 12-13 0.41144451960141737 1103 14-15 0.5269753427877646 1103 16-17 0.5186489863331332 1103 18-19 0.4124715657636031 1103 20-21 0.5511708216094391 1103 22-23 0.6071405338556204 1103 24-25 0.4979199390011573 1103 26-27 0.6989717945190108 1103 28-29 0.699045334516839 1103 30-31 0.6300115147076042 1103 32-33 0.6768954942626451 1103 34-35 0.47367913261944494 1103 36-37 0.5117048925805037 1103 38-39 0.5414942718780509 1103 40-41 0.6521792355206699 1103 42-43 0.6689137098708358 1103 44-45 0.6148343769764253 1103 46-47 0.7530332696683857 1103 48-49 0.9535015442805843 1103 50-51 1.034793439452855 1103 52-53 1.0448761715328914 1103 54-55 1.01834816968492 1103 56-57 1.1758688591651065 1103 58-59 1.1913769592703645 1103 60-61 1.108552777305249 1103 62-63 0.9817453342515137 1103 64-65 1.0591959302631366 1103 66-67 1.1423709783341138 1103 68-69 1.0233647032265552 1103 70-71 1.152270642085874 1103 72-73 1.020850603547693 1103 74-75 0.9777461354380108 1103 76-77 0.9821975297492429 1103 78-79 0.9451700708862916 1103 80-81 0.7958627275774859 1103 82-83 0.9891066225893823 1103 84-85 0.9431843300864138 1103 86-87 0.8507249627741906 1103 88-89 0.6537263950561858 1103 90-91 0.7499006837570832 1103 92-93 0.758106230438198 1103 94-95 0.8566400467160982 1103 96-97 1.013709472700043 1103 98-99 1.1674932612602653 1103 100-101 1.2443483911040296 1104 1 0.08763651357067204 1104 2 0.11720031120103869 1104 3 0.22109728354207192 1104 4 0.14217957782021529 1104 5 0.22711735026267377 1104 6 0.16052885060923927 1104 7 0.13261860536241699 1104 8 0.12208218735027998 1104 9 0.06017052736729056 1104 10-11 0.22015912242378022 1104 12-13 0.22300317331357888 1104 14-15 0.2707159303742088 1104 16-17 0.20560701179554286 1104 18-19 0.04401870212334558 1104 20-21 0.17465077453267952 1104 22-23 0.35755648488504477 1104 24-25 0.24989560145878897 1104 26-27 0.23590119449180946 1104 28-29 0.19243209090468127 1104 30-31 0.09928066650329015 1104 32-33 0.2215403451162885 1104 34-35 0.2173374534028909 1104 36-37 0.180653203844507 1104 38-39 0.10688714622978068 1104 40-41 0.1479937020540305 1104 42-43 0.10970343760129708 1104 44-45 -0.0036885813378120247 1104 46-47 -0.056737340264405134 1104 48-49 -0.11308335925738788 1104 50-51 0.04043291274384586 1104 52-53 0.053168329491875 1104 54-55 0.24089330309985257 1104 56-57 0.162691060456865 1104 58-59 0.15287777438327765 1104 60-61 0.1689340923317033 1104 62-63 0.3168767848503009 1104 64-65 0.24488049919348498 1104 66-67 0.20560980557886666 1104 68-69 0.1703754801777464 1104 70-71 0.09701589201745975 1104 72-73 0.12515483625865187 1104 74-75 0.05321669315656408 1104 76-77 0.26508704207498823 1104 78-79 0.17902437134144833 1104 80-81 0.3102241609002405 1104 82-83 0.2867537920237382 1104 84-85 0.15602114129081812 1104 86-87 0.1418822888008826 1104 88-89 0.02947665990491899 1104 90-91 0.12763290699225038 1104 92-93 0.03157367544955392 1104 94-95 -0.17342343303535657 1104 96-97 0.11536102074301624 1104 98-99 0.13350028248006396 1104 100-101 0.14898521674709642 1105 1 0.21792041815717056 1105 2 0.09822413890740478 1105 3 0.11515928820108456 1105 4 0.23328541955496007 1105 5 0.12831066079294118 1105 6 0.12027106473131255 1105 7 0.20757833634613831 1105 8 0.06633125843551824 1105 9 0.22145194843039206 1105 10-11 0.2593636569396125 1105 12-13 0.30330937827164206 1105 14-15 0.22415869061473614 1105 16-17 0.26786194835981547 1105 18-19 0.11536304552981846 1105 20-21 0.13335898941200242 1105 22-23 0.1799853643475231 1105 24-25 0.22796422884685086 1105 26-27 0.26383922833704077 1105 28-29 0.056776845023030376 1105 30-31 0.24510022922348185 1105 32-33 0.331600700510414 1105 34-35 0.06488874425749458 1105 36-37 0.310323447127125 1105 38-39 0.3366419384031545 1105 40-41 0.4585552542882354 1105 42-43 0.5203952625096946 1105 44-45 0.5822633076159605 1105 46-47 0.48227788174461494 1105 48-49 0.31344333380607026 1105 50-51 0.3445425984850843 1105 52-53 0.34742654533379635 1105 54-55 0.39058173963523757 1105 56-57 0.4228690142132763 1105 58-59 0.4974866996658065 1105 60-61 0.4298243745035961 1105 62-63 0.46511810374882145 1105 64-65 0.4051977099464352 1105 66-67 0.3069537639429498 1105 68-69 0.37749218652517413 1105 70-71 0.48012504511672915 1105 72-73 0.48654198354378053 1105 74-75 0.5858947592170054 1105 76-77 0.42199502336193007 1105 78-79 0.6137933779962665 1105 80-81 0.5782096512196304 1105 82-83 0.4800851787539031 1105 84-85 0.5458388558075633 1105 86-87 0.5360606083475652 1105 88-89 0.5399522193394226 1105 90-91 0.6605845135816075 1105 92-93 0.604901789319424 1105 94-95 0.6535673965384667 1105 96-97 0.6985285719376115 1105 98-99 0.8066498329430765 1105 100-101 1.1006591885234904 1106 1 0.07848142261991597 1106 2 0.04324407418815923 1106 3 0.2258579931902247 1106 4 0.05758111265804189 1106 5 0.05087132726348642 1106 6 0.11509634154067072 1106 7 0.11232869155089986 1106 8 0.10055922791607941 1106 9 0.1983558146856268 1106 10-11 0.20788478458175064 1106 12-13 0.23384337465387262 1106 14-15 0.287872567698237 1106 16-17 0.2933021206106545 1106 18-19 0.3027992807974229 1106 20-21 0.28963578624739483 1106 22-23 0.2250126540882107 1106 24-25 0.06684522429647899 1106 26-27 0.10753974221037055 1106 28-29 0.26474062978459756 1106 30-31 0.2809015836959361 1106 32-33 0.13767219837918532 1106 34-35 0.2200467243159565 1106 36-37 0.15142877551158307 1106 38-39 0.11369539750296553 1106 40-41 0.0995144323412731 1106 42-43 0.09264500745076276 1106 44-45 0.2529485868037824 1106 46-47 0.17471358498745815 1106 48-49 0.22351315423206586 1106 50-51 0.20698916129217793 1106 52-53 0.1505750365741605 1106 54-55 0.2412674256655194 1106 56-57 0.30905170525710446 1106 58-59 0.3986497996802001 1106 60-61 0.5782514589453172 1106 62-63 0.5119926147554779 1106 64-65 0.4271249539955875 1106 66-67 0.5610636552221209 1106 68-69 0.6376342997275231 1106 70-71 0.6305957305523933 1106 72-73 0.5581752056734999 1106 74-75 0.6436374407469856 1106 76-77 0.6644418365336122 1106 78-79 0.5701219690069195 1106 80-81 0.6146009272653785 1106 82-83 0.7216478919549942 1106 84-85 0.6021669773846021 1106 86-87 0.46422083542824666 1106 88-89 0.48874718325562583 1106 90-91 0.41151399213475415 1106 92-93 0.4558847833044162 1106 94-95 0.45095984929779576 1106 96-97 0.6508231634364812 1106 98-99 0.7229631204179618 1106 100-101 1.0265512688654859 1107 1 -0.18772613582263276 1107 2 0.01827490032872703 1107 3 -0.07967037321500214 1107 4 -0.004783029866779032 1107 5 0.06565039599625067 1107 6 0.06437462849707742 1107 7 0.05187290958387791 1107 8 0.03747051219029629 1107 9 0.18317155699180887 1107 10-11 0.09253781571297282 1107 12-13 -0.029694703574605796 1107 14-15 -0.008195828198395816 1107 16-17 -0.27340787214361484 1107 18-19 -0.1272484202716342 1107 20-21 0.053272381007580805 1107 22-23 0.005584558444546417 1107 24-25 0.0013007456713935994 1107 26-27 0.04334430447917015 1107 28-29 0.028795919804551318 1107 30-31 0.012977930830061268 1107 32-33 0.19401173719469256 1107 34-35 0.1404715848038265 1107 36-37 0.058746964841290605 1107 38-39 0.1452040033987032 1107 40-41 0.21386034612977767 1107 42-43 -0.07441281221949225 1107 44-45 0.13181456618522702 1107 46-47 0.1806595343690276 1107 48-49 0.22327639676069566 1107 50-51 0.25330940993519135 1107 52-53 0.004041229514022859 1107 54-55 0.22384747829242002 1107 56-57 0.09387809370461753 1107 58-59 0.1448354620290857 1107 60-61 0.178480112637466 1107 62-63 0.20549894523713164 1107 64-65 -0.027477992602101153 1107 66-67 0.030088150881041997 1107 68-69 0.2702979325087398 1107 70-71 0.3533948394544737 1107 72-73 0.21132153768056838 1107 74-75 0.007793471865504387 1107 76-77 0.2112435450009471 1107 78-79 0.17127937935687498 1107 80-81 0.07100100607860327 1107 82-83 0.18137449268973427 1107 84-85 -0.06964664840511503 1107 86-87 -0.15829480009871943 1107 88-89 0.040138649817208716 1107 90-91 0.1454542084897703 1107 92-93 0.03274992370636198 1107 94-95 0.20137536660492827 1107 96-97 0.23157255644752794 1107 98-99 0.3122492886969077 1107 100-101 0.25799997035403877 1108 1 -0.2542920796548387 1108 2 -0.054713605418399425 1108 3 -0.0870053655126668 1108 4 -0.24575944887376977 1108 5 -0.14839451453817532 1108 6 -0.21221916223642268 1108 7 -0.18898797440711945 1108 8 -0.24550140391350794 1108 9 -0.08239308336842299 1108 10-11 -0.31492159890962057 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-1.1334331799900923 2316 58-59 -1.2024375675685164 2316 60-61 -1.175988626970117 2316 62-63 -1.2256117963658042 2316 64-65 -1.0360746005169688 2316 66-67 -1.0017043333224862 2316 68-69 -1.1371180967563106 2316 70-71 -1.0389023005521736 2316 72-73 -0.9561917646140756 2316 74-75 -1.1096574759229938 2316 76-77 -1.4168824942408165 2316 78-79 -1.6099519142912726 2316 80-81 -1.398507640412916 2316 82-83 -1.5214356170541947 2316 84-85 -1.5330071405900156 2316 86-87 -1.7280154519111584 2316 88-89 -1.5092003924175792 2316 90-91 -1.6371987678986564 2316 92-93 -1.7109979978101002 2316 94-95 -1.5115361731356778 2316 96-97 -1.891407157553136 2316 98-99 -1.6645880907510424 2316 100-101 -1.9120823375940823 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3715.0 3 659.0 4 574.0 5 489.0 6 452.0 7 530.0 8 625.0 9 807.0 10 983.0 11 1504.0 12 2705.0 13 4645.0 14 4875.0 15 4419.0 16 5401.0 17 7427.0 18 6498.0 19 3301.0 20 2983.0 21 2936.0 22 3025.0 23 3421.0 24 3871.0 25 4467.0 26 5612.0 27 6641.0 28 7865.0 29 9705.0 30 11974.0 31 15063.0 32 19356.0 33 25882.0 34 35979.0 35 55145.0 36 86052.0 37 175709.0 38 288501.0 39 80770.0 40 119.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.063271082067224 22.296173789164232 12.54675554273331 23.093799586035228 2 14.730156175069 23.46169625061342 39.95615785408234 21.851989720235242 3 16.545793548415944 30.158662734936875 31.35159545809198 21.943948258555203 4 10.102163998926942 18.388513815612985 43.085039792542254 28.42428239291782 5 9.764669192153479 41.68126329485487 37.61414153382524 10.93992597916641 6 25.803624722512225 42.62145190100085 19.56945521855865 12.00546815792827 7 25.228472809053066 34.68315605452944 25.352098507102326 14.736272629315165 8 24.37675917004062 36.20732440716389 23.16258252309976 16.25333389969573 9 26.64545684218057 15.482911037231961 23.811418874657093 34.06021324593038 10-11 21.81496883472091 29.025205509768735 31.296054223617652 17.8637714318927 12-13 22.607991915976232 27.238979481424185 31.141097066411504 19.01193153618808 14-15 20.816971436683513 28.96444144872229 28.22803034621829 21.990556768375903 16-17 18.33816461844772 32.09698641836879 30.06818204684763 19.496666916335855 18-19 18.142185721064607 31.012979783329335 32.684931032150715 18.15990346345534 20-21 18.354607947241938 30.818085284748225 32.78975018163528 18.03755658637456 22-23 18.717066507490447 30.150367871442846 32.5301305826252 18.602435038441502 24-25 18.491946595714353 30.821271160623702 32.544026115221115 18.14275612844083 26-27 17.87696758198398 31.02765848522176 32.85121380330999 18.24416012948428 28-29 18.033267954091812 29.646924147969116 33.65553430900998 18.66427358892909 30-31 18.240943302789418 30.728493033997754 32.913927421220954 18.116636241991866 32-33 17.81695828477689 31.174269417686002 32.99568790853783 18.013084388999275 34-35 18.29455470372888 30.712103067976965 33.11777716385986 17.875565064434294 36-37 18.34709936250219 30.169673703552284 32.25833262200737 19.224894311938144 38-39 17.756838257177996 31.302231032294657 32.74833079700583 18.192599913521526 40-41 17.737667774821574 30.745934846332666 33.58063217064125 17.9357652082045 42-43 18.002006069877726 30.435371077998603 32.538374791361996 19.02424806076168 44-45 17.580909061803688 31.138312804748246 32.55982066578557 18.720957467662494 46-47 18.304552925355928 30.72014119270085 32.19655309162359 18.77875279031963 48-49 18.170935244685555 31.299235268111193 31.84830523661298 18.681524250590275 50-51 18.34064545561292 30.782377420121453 31.628988028120347 19.24798909614528 52-53 17.933016128035593 31.328891710155997 32.00872969951632 18.72936246229208 54-55 18.622380917877244 31.103992648624118 31.715390314360064 18.55823611913857 56-57 18.639330763899263 31.375834214212617 31.077364978336547 18.90747004355157 58-59 17.94938617055573 30.850251226928744 32.27460063736266 18.925761965152866 60-61 18.599714016451355 32.036503901664794 31.02034122577536 18.34344085610849 62-63 18.419112092819766 31.645277436594704 31.91940329186906 18.016207178716467 64-65 18.386500093524667 30.847243606198806 31.677619069819958 19.088637230456573 66-67 18.325472320605773 31.411910515300196 31.1347768245477 19.12784033954633 68-69 18.299435815281743 31.024935571498226 31.657312269838023 19.018316343382008 70-71 18.73275173619886 30.35250761815868 32.36748672087269 18.547253924769773 72-73 18.580472752638915 30.978743767081202 31.153754129536875 19.28702935074301 74-75 18.393476344897202 31.113525282421534 31.16806882710033 19.324929545580936 76-77 18.110094700831084 31.001842793587457 32.196485268603595 18.691577236977864 78-79 17.709075774961626 30.76217548104601 31.816632152784447 19.712116591207916 80-81 18.233895228274033 31.35052493703676 30.8057609396519 19.609818895037307 82-83 19.12246922289711 30.61635087301489 30.8909548624958 19.3702250415922 84-85 18.115683577371488 30.608276496567328 31.312953165905693 19.963086760155495 86-87 18.42618903053788 30.76766239098529 31.494642432875246 19.31150614560158 88-89 18.844919065127083 30.306521696388195 31.463400247734018 19.3851589907507 90-91 18.69525322377001 30.172655507675294 31.738506723995613 19.39358454455908 92-93 19.30754843447223 30.660735926430956 31.14133039380767 18.890385245289146 94-95 19.043187219130864 29.54980619643442 31.378709747356087 20.028296837078628 96-97 19.460071976177893 29.65780150904026 31.116978415569495 19.76514809921235 98-99 18.866366164032218 29.874015730347175 31.372261675358338 19.887356430262276 100-101 18.97699553926391 29.91798399885 30.943436698426392 20.1615837634597 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 484.0 1 342.5 2 405.0 3 708.5 4 603.0 5 584.5 6 1255.5 7 2117.5 8 2788.0 9 2860.5 10 2277.5 11 1869.0 12 1888.0 13 1932.0 14 1877.0 15 1824.0 16 1822.0 17 1760.0 18 1699.0 19 1750.5 20 1983.0 21 2373.5 22 2738.5 23 3405.0 24 3954.0 25 4784.5 26 6764.0 27 9256.5 28 10182.0 29 11719.5 30 17115.5 31 21943.5 32 27771.0 33 35884.5 34 42145.0 35 47783.0 36 57451.5 37 62941.5 38 58733.5 39 55217.0 40 49731.0 41 42511.0 42 35374.0 43 33297.5 44 33826.0 45 32024.0 46 28002.0 47 21623.5 48 18702.0 49 16121.5 50 13564.0 51 10493.0 52 7759.0 53 6324.5 54 5426.5 55 4875.5 56 4078.5 57 3255.0 58 2757.0 59 2375.0 60 1973.5 61 1591.5 62 1058.0 63 649.0 64 385.5 65 241.0 66 222.0 67 256.5 68 288.0 69 236.5 70 172.5 71 150.5 72 111.5 73 99.5 74 92.0 75 60.0 76 37.0 77 21.5 78 16.5 79 13.0 80 20.0 81 29.0 82 20.0 83 9.5 84 6.5 85 4.5 86 5.0 87 5.0 88 5.0 89 4.0 90 2.0 91 1.5 92 4.0 93 4.0 94 2.5 95 4.0 96 3.5 97 2.0 98 2.0 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.045379099906671064 2 0.012518372388047191 3 0.009277008108999257 4 0.005029703191626103 5 0.008047525106601764 6 0.005700330283842917 7 0.005476787919770646 8 0.009724092837143799 9 0.014642024846733766 10-11 0.0021795380497046444 12-13 0.009053465744926985 14-15 0.008494609834746307 16-17 0.018777558582070787 18-19 0.011344774976667766 20-21 0.003911991371264747 22-23 0.008829923380854714 24-25 0.02095709663177543 26-27 0.006594499740132001 28-29 0.017939274716799767 30-31 0.014083168936553089 32-33 0.01201540206888458 34-35 0.27043037493643013 36-37 0.4838574470344311 38-39 0.47949837093502184 40-41 0.4992259845643997 42-43 0.49034017559252696 44-45 0.5033615182997369 46-47 0.5090059629925616 48-49 0.5180035431464706 50-51 0.5280629495297228 52-53 0.5163828610069466 54-55 0.5042556877560259 56-57 0.5002878107937431 58-59 0.49782884478894807 60-61 0.494475709327864 62-63 0.4918490865500148 64-65 0.5080559079452545 66-67 0.48290739198712396 68-69 0.5086706494464532 70-71 0.4893342349542018 72-73 0.4976053024248758 74-75 0.5108501874961578 76-77 0.49855535747218294 78-79 0.4988906710182914 80-81 0.5010702090679959 82-83 0.5022996920703935 84-85 0.5017967217512308 86-87 0.5024673488434477 88-89 0.5161034330518562 90-91 0.5420343472842397 92-93 0.5304101443524816 94-95 0.5006790099308696 96-97 0.4751951804266306 98-99 0.4668682273649385 100-101 0.4749157524715403 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 894685.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.820224687879424 #Duplication Level Percentage of deduplicated Percentage of total 1 68.26128184802623 15.57737789254624 2 12.513836707906833 5.711371307637346 3 5.4862948072245645 3.7559544061443213 4 2.9836634292804134 2.723514793967515 5 1.9255946536629889 2.1971251327184387 6 1.3769224997286202 1.885300849296223 7 0.9160792843934719 1.4633594571239574 8 0.7446272214958085 1.3594048402596568 9 0.5585129496779958 1.1470851902467942 >10 4.049723982083686 18.426873195464665 >50 0.5628305241673708 9.112140148400979 >100 0.5682188059418707 25.876528517792213 >500 0.040167191454511544 6.238969994831424 >1k 0.011756251157418013 3.943384955255374 >5k 4.898437982257506E-4 0.5816093183148711 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5203 0.5815454601340136 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3735 0.4174653649049665 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2816 0.3147476486137579 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1752 0.1958231109273096 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 1750 0.19559956856323732 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1692 0.18911684000514148 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 1613 0.18028691662428678 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1549 0.1731335609739741 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 1488 0.1663155188697698 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1440 0.1609505021320353 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1330 0.14865567210806038 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 1309 0.14630847728530152 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1306 0.1459731637391931 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1298 0.14507899428290405 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1250 0.1397139775451695 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 1242 0.13881980808888045 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1226 0.13703146917630227 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1108 0.12384246969603828 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1103 0.12328361378585759 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 1092 0.1220541307834601 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 1065 0.11903630886848444 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 1051 0.11747151231997854 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 1041 0.11635380049961719 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1013 0.11322420740260539 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1008 0.11266535149242471 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 999 0.11165941085409949 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 990 0.11065347021577426 No Hit CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA 989 0.11054169903373812 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 984 0.10998284312355745 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 967 0.10808273302894315 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 947 0.10584730938822044 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 909 0.10160000447084729 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.1177118203613562E-4 0.0 12-13 0.0 0.0 0.0 1.1177118203613562E-4 0.0 14-15 0.0 0.0 0.0 1.1177118203613562E-4 0.0 16-17 0.0 0.0 0.0 1.1177118203613562E-4 0.0 18-19 0.0 0.0 0.0 2.2354236407227124E-4 0.0 20-21 0.0 0.0 0.0 2.2354236407227124E-4 0.0 22-23 0.0 0.0 0.0 0.0010618262293432884 0.0 24-25 0.0 0.0 0.0 0.0022354236407227126 0.0 26-27 0.0 0.0 0.0 0.003185478688029865 0.0 28-29 0.0 0.0 0.0 0.006091529420969391 0.0 30-31 0.0 0.0 0.0 0.012686029161101394 0.0 32-33 0.0 0.0 0.0 0.025539715095256987 0.0 34-35 0.0 0.0 0.0 0.0503529175072791 0.0 36-37 0.0 0.0 0.0 0.0944466488205346 0.0 38-39 0.0 0.0 0.0 0.16301826899970379 0.0 40-41 0.0 0.0 0.0 0.24299054974655887 0.0 42-43 0.0 0.0 0.0 0.3196655806233479 0.0 44-45 0.0 0.0 0.0 0.4118768058031598 0.0 46-47 0.0 0.0 0.0 0.5248215852506748 0.0 48-49 0.0 0.0 0.0 0.6536937581383392 0.0 50-51 0.0 0.0 0.0 0.7893839731302079 0.0 52-53 0.0 0.0 0.0 0.9192062010651794 0.0 54-55 0.0 0.0 0.0 1.0660735342606618 0.0 56-57 0.0 0.0 0.0 1.2333391081777385 0.0 58-59 0.0 0.0 0.0 1.4227912617289884 0.0 60-61 0.0 0.0 0.0 1.6239793893940324 0.0 62-63 0.0 0.0 0.0 1.8150522250848065 0.0 64-65 0.0 0.0 0.0 2.037979847655879 0.0 66-67 0.0 0.0 0.0 2.295891850204262 0.0 68-69 0.0 0.0 0.0 2.5778346568904142 0.0 70-71 0.0 0.0 0.0 2.8754813146526432 0.0 72-73 0.0 0.0 0.0 3.1641303922609634 0.0 74-75 0.0 0.0 0.0 3.473960108865131 0.0 76-77 0.0 0.0 0.0 3.820897857905296 0.0 78-79 0.0 0.0 0.0 4.211482253530573 0.0 80-81 0.0 0.0 0.0 4.6402923934122065 0.0 82-83 0.0 0.0 0.0 5.056807703269866 0.0 84-85 0.0 0.0 0.0 5.4787998010472965 0.0 86-87 0.0 0.0 0.0 5.934267367844548 0.0 88-89 0.0 0.0 0.0 6.444055729111364 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTGCA 25 0.005097355 56.782806 9 GGTATCA 1890 0.0 42.831585 1 CATTCCG 165 0.0 40.14946 9 GTATCAA 3290 0.0 40.001728 1 TCAGGCG 50 0.0016457577 37.857323 3 ACAACGC 65 1.3576649E-4 36.401264 3 TACCGCG 175 0.0 35.15126 9 GTTCCGT 55 0.0026223043 34.429214 1 GTACATG 4710 0.0 34.273884 1 ATGGGTA 565 0.0 33.502056 5 CATGGGG 1330 0.0 33.445377 4 CATGGGT 705 0.0 32.890224 4 TCAACGC 3995 0.0 32.455902 4 ATCAACG 4040 0.0 32.32865 3 CAACGCA 4060 0.0 31.936287 5 CAGGCGT 75 3.1465667E-4 31.547768 4 GATACCG 195 0.0 31.546005 7 ACATGGG 4805 0.0 31.51494 3 AACGCAG 4235 0.0 30.726624 6 AGTACAT 2975 0.0 30.381294 2 >>END_MODULE