FastQCFastQC Report
Thu 26 May 2016
SRR936365_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936365_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894685
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52660.5885870446022903No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37100.41467108535406316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28090.31396525033950495No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19040.2128123305968022No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA17360.19403477201473143No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT16620.1857637045440574No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT15880.17749263707338336No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA14750.16486249350330004No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG14410.16106227331407144No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG14220.15893862085538485No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA13340.14910275683620494No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC12660.1415023164577477No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA12570.14049637581942248No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT12190.13624907090204932No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG11990.1340136472613266No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC11870.132672393076893No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT11790.1317782236206039No Hit
GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG11520.12876040170562822No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA11150.12462486797029121No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG11040.12339538496789373No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT10450.11680088522776173No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10290.11501254631518355No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG10290.11501254631518355No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC10020.1119947244002079No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG9870.11031815666966585No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC9770.1092004448493045No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG9770.1092004448493045No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA9580.10707679239061793No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT9420.10528845347803975No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT9180.10260594510917251No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9160.10238240274510024No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATGC2900.047.4371837
TGGGCGA405.422799E-447.437186
GGTATCA18650.043.1185071
GTATCAA32400.040.680911
CATTCCG1750.040.660449
GGATGCC3400.040.461138
GTACATG42250.038.2921
TAACGCA500.001626162137.9497454
CACAGCG651.3383287E-436.4901357
ACATGGG44100.036.035053
ATGGGTA5800.035.9868285
CATGGGT7550.035.8135034
GGTATGC2450.034.8518078
ATGGGAT12800.034.0954745
GGGTATG3900.034.0574657
CATGGGA22500.033.7331054
TACATGG48450.033.0084342
CATGGGG13050.031.988294
GTCATAC1201.4821853E-731.72231
TCATACG753.0947776E-431.6371652
Produced by FastQC (version 0.11.5)