##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936365_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 894685 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45179141262008 34.0 31.0 34.0 31.0 34.0 2 32.589395150248414 34.0 31.0 34.0 31.0 34.0 3 32.67590045658528 34.0 31.0 34.0 31.0 34.0 4 36.171673829336584 37.0 35.0 37.0 35.0 37.0 5 36.15364625538597 37.0 35.0 37.0 35.0 37.0 6 36.19815130464912 37.0 37.0 37.0 35.0 37.0 7 36.15208481197293 37.0 36.0 37.0 35.0 37.0 8 36.1384912008137 37.0 36.0 37.0 35.0 37.0 9 37.898525179253035 39.0 38.0 39.0 35.0 39.0 10-11 37.839188652989606 39.0 38.0 39.0 35.0 39.0 12-13 37.824801466437904 39.0 38.0 39.0 35.0 39.0 14-15 39.146518607107524 40.5 38.5 41.0 36.0 41.0 16-17 39.08481979691176 40.0 38.0 41.0 36.0 41.0 18-19 39.184377183030904 40.0 38.5 41.0 36.0 41.0 20-21 39.15723131604978 40.0 39.0 41.0 36.0 41.0 22-23 39.037765805842284 40.0 38.5 41.0 35.0 41.0 24-25 39.185275823334464 41.0 39.0 41.0 36.0 41.0 26-27 39.083907185210435 41.0 39.0 41.0 35.0 41.0 28-29 39.00115627287816 40.5 39.0 41.0 35.0 41.0 30-31 38.871340192358204 40.0 38.0 41.0 35.0 41.0 32-33 38.681035783543926 40.0 38.0 41.0 35.0 41.0 34-35 38.5341164767488 40.0 38.0 41.0 35.0 41.0 36-37 38.38698089271643 40.0 38.0 41.0 34.0 41.0 38-39 38.261209811274355 40.0 38.0 41.0 34.0 41.0 40-41 38.14069141653208 40.0 38.0 41.0 34.0 41.0 42-43 38.11480968161979 40.0 38.0 41.0 34.0 41.0 44-45 37.95298680541197 40.0 38.0 41.0 33.0 41.0 46-47 37.721905475111356 40.0 38.0 41.0 33.0 41.0 48-49 37.49549729793168 40.0 38.0 41.0 33.0 41.0 50-51 37.35548880332183 40.0 38.0 41.0 33.0 41.0 52-53 37.22875593085834 40.0 38.0 41.0 32.5 41.0 54-55 37.1193995652101 40.0 37.0 41.0 32.0 41.0 56-57 36.951424803143006 40.0 37.0 41.0 31.5 41.0 58-59 36.78520876062525 40.0 37.0 41.0 31.0 41.0 60-61 36.62063798990706 40.0 36.0 41.0 31.0 41.0 62-63 36.38034056679166 39.0 36.0 41.0 31.0 41.0 64-65 36.078830538122354 39.0 35.5 41.0 30.0 41.0 66-67 35.790445240503644 39.0 35.0 40.0 29.5 41.0 68-69 35.457972917842596 38.0 35.0 40.0 29.0 41.0 70-71 35.12027193928589 37.5 35.0 40.0 29.0 41.0 72-73 34.70930495090451 37.0 34.5 39.0 28.5 41.0 74-75 34.25056975360043 36.5 34.0 39.0 28.0 40.5 76-77 33.44386739466964 36.0 33.5 38.0 26.5 40.0 78-79 33.43782951541604 35.5 34.0 37.5 27.0 39.0 80-81 33.20438869546265 35.0 34.0 37.0 27.0 39.0 82-83 32.845352274822986 35.0 34.0 37.0 26.0 39.0 84-85 32.440481845565756 35.0 33.5 36.0 26.0 37.5 86-87 32.02415822328529 35.0 33.0 36.0 25.5 37.0 88-89 31.48519143609203 35.0 33.0 35.5 23.5 37.0 90-91 31.225162487355888 35.0 32.0 35.0 22.5 36.0 92-93 30.9291979858833 35.0 32.0 35.0 20.0 36.0 94-95 30.770940051526516 35.0 32.0 35.0 20.0 36.0 96-97 30.56297691366235 34.5 32.0 35.0 18.0 35.5 98-99 30.21351425361999 34.0 32.0 35.0 2.0 35.0 100-101 27.09508653883769 31.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04934880252071849 1101 2 0.09755349022054105 1101 3 0.18866770519554166 1101 4 0.10475670195981479 1101 5 0.07245589589504675 1101 6 0.04492983824576413 1101 7 0.10177673482722582 1101 8 0.029633714364109665 1101 9 0.004245196165435061 1101 10-11 0.03042922748868193 1101 12-13 0.15291795561275023 1101 14-15 0.2716238975072969 1101 16-17 0.33478672618535654 1101 18-19 0.26965617106410633 1101 20-21 0.3123202273344461 1101 22-23 0.3376586503150918 1101 24-25 0.36958838659249693 1101 26-27 0.3874604623482796 1101 28-29 0.3427660184389154 1101 30-31 0.27035399429603046 1101 32-33 0.35524803896117163 1101 34-35 0.2774091410349939 1101 36-37 0.3254905571932909 1101 38-39 0.28464836543675887 1101 40-41 0.4727002148759638 1101 42-43 0.3538342093949325 1101 44-45 0.3705084068839497 1101 46-47 0.3940338135820838 1101 48-49 0.39671163364346995 1101 50-51 0.3119037188923741 1101 52-53 0.22455331898090947 1101 54-55 0.4221132985807543 1101 56-57 0.4021764854324559 1101 58-59 0.22350989795367582 1101 60-61 0.18678401327001382 1101 62-63 0.22193462635813432 1101 64-65 0.33698639904753236 1101 66-67 0.11107345031737026 1101 68-69 0.42492663609072423 1101 70-71 0.27643722850945807 1101 72-73 0.20977415983895753 1101 74-75 0.25319024077609953 1101 76-77 0.167124894238583 1101 78-79 0.21878156008792615 1101 80-81 0.39094502663547104 1101 82-83 0.3347046789192376 1101 84-85 0.21674265975002527 1101 86-87 0.38749644492938273 1101 88-89 0.39295960823221776 1101 90-91 0.3877421681040296 1101 92-93 0.4423637311126427 1101 94-95 0.30042233825712117 1101 96-97 0.3892000373269937 1101 98-99 0.572188577902299 1101 100-101 0.7543991611779042 1102 1 0.15510411318340545 1102 2 0.14643522272267973 1102 3 0.06601281702229045 1102 4 0.07352793722438378 1102 5 0.11510369333647219 1102 6 0.10674477014519113 1102 7 0.10156253563675222 1102 8 0.20052773746807162 1102 9 0.10597503928299545 1102 10-11 0.252295271746668 1102 12-13 0.23093077801946293 1102 14-15 0.30571111350872116 1102 16-17 0.1925399982626459 1102 18-19 0.43396541560571933 1102 20-21 0.25443843574286973 1102 22-23 0.38022889287720574 1102 24-25 0.23088333149160434 1102 26-27 0.2229850287795685 1102 28-29 0.2905457330369643 1102 30-31 0.31430914541724775 1102 32-33 0.2484512770620384 1102 34-35 0.3614601647594 1102 36-37 0.11197458827432882 1102 38-39 0.10100579741004623 1102 40-41 0.229691233282324 1102 42-43 0.3766427300910067 1102 44-45 0.3143697535321053 1102 46-47 0.3841806508202481 1102 48-49 0.25413252766657024 1102 50-51 -0.04036531529086318 1102 52-53 0.23681806918033033 1102 54-55 0.22501311244904088 1102 56-57 0.3374440128351779 1102 58-59 0.027410539074011808 1102 60-61 0.1538158033843544 1102 62-63 0.26114415748348563 1102 64-65 0.16230326612586055 1102 66-67 0.1349197291603943 1102 68-69 0.08036138658560077 1102 70-71 0.30068236101750756 1102 72-73 0.23912683511299093 1102 74-75 0.26655922473332083 1102 76-77 0.09390200890162959 1102 78-79 0.28921616285921203 1102 80-81 0.26631802867405696 1102 82-83 0.3254424104759508 1102 84-85 0.43336821002035464 1102 86-87 0.41506535797928024 1102 88-89 0.4804156604672727 1102 90-91 0.6243030154464826 1102 92-93 0.5173408339577961 1102 94-95 0.40872588066855897 1102 96-97 0.21084966654080972 1102 98-99 0.5518987442390504 1102 100-101 0.9454463736339598 1103 1 0.15349024778937093 1103 2 0.20104743341644848 1103 3 0.2653260809008273 1103 4 0.04646684114524646 1103 5 0.12508515785273744 1103 6 0.1960968094034854 1103 7 0.21246473787947906 1103 8 0.07711230827575122 1103 9 0.17997138899999499 1103 10-11 0.25667525626491994 1103 12-13 0.31124321809872413 1103 14-15 0.4131920631504329 1103 16-17 0.36283475506431984 1103 18-19 0.3244755675114632 1103 20-21 0.2313622911234816 1103 22-23 0.4254703867945864 1103 24-25 0.3565411281126103 1103 26-27 0.30281011346755804 1103 28-29 0.3644701778601629 1103 30-31 0.35908103823811643 1103 32-33 0.3546042054554164 1103 34-35 0.21481146543903407 1103 36-37 0.3192328990597133 1103 38-39 0.3947870005831007 1103 40-41 0.5067390136403915 1103 42-43 0.6641353620586798 1103 44-45 0.7427826616145623 1103 46-47 0.8050710642112406 1103 48-49 0.6043075850129469 1103 50-51 0.6075865492651715 1103 52-53 0.488029799797971 1103 54-55 0.625107032668339 1103 56-57 0.764329163853219 1103 58-59 0.7575798795374737 1103 60-61 0.7216088025108434 1103 62-63 0.7315646146920898 1103 64-65 0.7090964854055954 1103 66-67 0.781988317551928 1103 68-69 0.8005181925169538 1103 70-71 0.6802345621949755 1103 72-73 0.6543136813110806 1103 74-75 0.7503681956233734 1103 76-77 0.8286537328436481 1103 78-79 0.6860716997135796 1103 80-81 0.74644475883008 1103 82-83 0.7226574717992875 1103 84-85 0.8146035183414355 1103 86-87 0.6583311539753325 1103 88-89 0.6053100224134269 1103 90-91 0.7526701174484565 1103 92-93 0.9272244723099163 1103 94-95 0.9305308177828451 1103 96-97 0.8741891783687201 1103 98-99 0.7119004486513454 1103 100-101 1.2328573358457184 1104 1 -0.07833592413481938 1104 2 0.04739319239486406 1104 3 -0.049900161201982485 1104 4 0.04338603331881785 1104 5 0.010474069966612376 1104 6 0.019799050586250644 1104 7 0.10179070413665414 1104 8 0.15550417695779117 1104 9 0.1269428793554681 1104 10-11 0.06577681883050701 1104 12-13 0.14494759150269942 1104 14-15 0.0824811940268475 1104 16-17 0.04752708561569108 1104 18-19 0.11457937478552793 1104 20-21 0.07879178931277409 1104 22-23 0.11230836988119819 1104 24-25 0.08807013219740156 1104 26-27 0.28522432514171925 1104 28-29 0.2459438617172438 1104 30-31 0.10562877061245501 1104 32-33 0.16618216668256736 1104 34-35 0.18463633788351785 1104 36-37 0.12494977030208787 1104 38-39 0.24369646176911175 1104 40-41 0.4300781056499332 1104 42-43 0.3773314609333056 1104 44-45 0.31767735171216316 1104 46-47 0.3839305105388675 1104 48-49 0.3848071627659877 1104 50-51 0.44447246535639096 1104 52-53 0.30204806302823783 1104 54-55 0.23996659987780333 1104 56-57 0.424451422395947 1104 58-59 0.30330259340178145 1104 60-61 0.29188647815466595 1104 62-63 0.4287153291540875 1104 64-65 0.3264290934479348 1104 66-67 0.26044029136757985 1104 68-69 0.4106919097844255 1104 70-71 0.3440504323007261 1104 72-73 -0.045491396733247313 1104 74-75 0.10290579071288875 1104 76-77 0.1712790176295016 1104 78-79 -0.09364466449666509 1104 80-81 0.022329001043694063 1104 82-83 0.13389410973972105 1104 84-85 0.10732978982525054 1104 86-87 0.14040118820839353 1104 88-89 0.17648507777562372 1104 90-91 0.27080071347984 1104 92-93 0.11964736397125009 1104 94-95 0.2689084492712226 1104 96-97 0.17324411748289137 1104 98-99 -0.023778831698809455 1104 100-101 0.26936029157442576 1105 1 0.004441771643193704 1105 2 0.10453669127502963 1105 3 0.15314873276376062 1105 4 0.048126867705668985 1105 5 0.07211935608518871 1105 6 0.1235679738152129 1105 7 0.05597226445675574 1105 8 0.1149255224242367 1105 9 0.17038368229719225 1105 10-11 0.12080418892683298 1105 12-13 0.1945121727370207 1105 14-15 0.1939918757443948 1105 16-17 0.2418491543200858 1105 18-19 0.15723084083442274 1105 20-21 0.07929795961702268 1105 22-23 -0.02645288458689521 1105 24-25 0.15913531987348506 1105 26-27 0.2082214629933148 1105 28-29 0.06379554980723157 1105 30-31 0.21177853926615597 1105 32-33 0.16845792762258327 1105 34-35 0.3241355902595515 1105 36-37 0.12406096412600931 1105 38-39 0.33481032522025345 1105 40-41 0.3495336557150708 1105 42-43 0.283185388686519 1105 44-45 0.38576487826404815 1105 46-47 0.4494647247636223 1105 48-49 0.5370697680911221 1105 50-51 0.39334284725426727 1105 52-53 0.5309798270999337 1105 54-55 0.38131485788187547 1105 56-57 0.5970330353385123 1105 58-59 0.59916194383527 1105 60-61 0.657477004828479 1105 62-63 0.563571612083237 1105 64-65 0.5066971150359763 1105 66-67 0.6469009175748468 1105 68-69 0.5083113852283461 1105 70-71 0.43129461976503336 1105 72-73 0.4790831314293911 1105 74-75 0.4898653676796485 1105 76-77 0.3218771168035772 1105 78-79 0.23992397251745956 1105 80-81 0.422134554638383 1105 82-83 0.4362590721660027 1105 84-85 0.427101074647517 1105 86-87 0.34899068168513736 1105 88-89 0.5379837269185828 1105 90-91 0.686845429735687 1105 92-93 0.7491162621724605 1105 94-95 0.5145037248011342 1105 96-97 0.4297040056922512 1105 98-99 0.7566579668020914 1105 100-101 1.0112631710497375 1106 1 -0.1007676896347931 1106 2 0.022082241983248707 1106 3 0.05324387130927022 1106 4 -0.040344155722195296 1106 5 0.030396096588546584 1106 6 0.08375374434354654 1106 7 0.05127636355106091 1106 8 -0.009510374851174674 1106 9 0.02763048545428859 1106 10-11 0.10267309802570423 1106 12-13 0.16050953139055935 1106 14-15 0.1678045216600097 1106 16-17 0.004158486034398834 1106 18-19 0.14553411998468135 1106 20-21 0.22676661709947865 1106 22-23 0.2289862862055756 1106 24-25 0.171716923083153 1106 26-27 0.20777471867189234 1106 28-29 0.24415898531018598 1106 30-31 0.15436680843313155 1106 32-33 0.18637699100863614 1106 34-35 0.2497788707868196 1106 36-37 0.11940418268804365 1106 38-39 0.29735265248432796 1106 40-41 0.1957503337524642 1106 42-43 0.2742661500452428 1106 44-45 0.24716382172545792 1106 46-47 0.20480218494000013 1106 48-49 0.1611496782102364 1106 50-51 0.26436674455470666 1106 52-53 0.1181333068548227 1106 54-55 0.23661742349304404 1106 56-57 0.2863970779755931 1106 58-59 0.35601802174873143 1106 60-61 0.36270164445993913 1106 62-63 0.45406057413422474 1106 64-65 0.43513274316944717 1106 66-67 0.38924445535173646 1106 68-69 0.4014510824123718 1106 70-71 0.457914635162453 1106 72-73 0.2679921364179094 1106 74-75 0.548042275301377 1106 76-77 0.2169321031274123 1106 78-79 0.14369947058651888 1106 80-81 0.26711505749567266 1106 82-83 0.48376184182432524 1106 84-85 0.4548039169115654 1106 86-87 0.290474999472913 1106 88-89 0.3802587273880178 1106 90-91 0.582405839582524 1106 92-93 0.48527422971269374 1106 94-95 0.43841870724431686 1106 96-97 0.3424558568254632 1106 98-99 0.3614723214560591 1106 100-101 0.7165903036866403 1107 1 0.05715907762466799 1107 2 0.11974549341015006 1107 3 0.019703759191102677 1107 4 0.03395160997371249 1107 5 -0.11854342061688783 1107 6 2.752845065145948E-4 1107 7 0.0031764614319982343 1107 8 0.09251461656835858 1107 9 0.024052110786385583 1107 10-11 0.1222302185463846 1107 12-13 0.08925744983158523 1107 14-15 -0.04335374673716075 1107 16-17 -0.05797684390064717 1107 18-19 0.128170581270858 1107 20-21 0.11293394266166246 1107 22-23 0.14681952631896422 1107 24-25 0.21757636859398133 1107 26-27 0.24154999928511245 1107 28-29 0.08400526774119754 1107 30-31 0.09690465784902358 1107 32-33 0.06497920032131077 1107 34-35 0.0018103770092494642 1107 36-37 0.07146794512972576 1107 38-39 0.0729343767143078 1107 40-41 0.0990921098466302 1107 42-43 0.16607434153596756 1107 44-45 0.03258051487506464 1107 46-47 0.01757072193772302 1107 48-49 -0.14043167102133225 1107 50-51 0.01927827035332541 1107 52-53 0.2111861434953468 1107 54-55 0.2037708995376306 1107 56-57 0.20212095202697355 1107 58-59 0.30547506854090756 1107 60-61 0.2756837398747862 1107 62-63 0.35118150794382075 1107 64-65 0.2938981721294951 1107 66-67 0.2806140893787088 1107 68-69 0.3380382155177344 1107 70-71 0.40815107483855684 1107 72-73 0.21330195502036986 1107 74-75 0.16362838510600142 1107 76-77 -0.040890427285589226 1107 78-79 0.12640743580701042 1107 80-81 0.27585204377962924 1107 82-83 0.29731930556388164 1107 84-85 0.28954481843316415 1107 86-87 0.3128787710670977 1107 88-89 0.4771354355375621 1107 90-91 0.25754837075824 1107 92-93 0.48125491411656895 1107 94-95 0.38507518327620005 1107 96-97 0.40256365297886987 1107 98-99 0.3743152225454587 1107 100-101 0.7056970765392983 1108 1 -0.1695532105611406 1108 2 -0.05195435254316294 1108 3 -0.10821424057190399 1108 4 -0.12026393689203019 1108 5 -0.17938889993908447 1108 6 -0.18741283019902255 1108 7 -0.1816232778736051 1108 8 -0.16284739314843222 1108 9 -0.1662666473603096 1108 10-11 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64-65 -0.9297508135705428 2316 66-67 -0.9613089235910266 2316 68-69 -0.870475253055595 2316 70-71 -1.0181916255272583 2316 72-73 -1.240992191969987 2316 74-75 -1.154874275352931 2316 76-77 -1.0488593331718619 2316 78-79 -1.3088561159056624 2316 80-81 -1.372160925957651 2316 82-83 -1.3922758058428357 2316 84-85 -1.2961427439213367 2316 86-87 -1.2313638295314995 2316 88-89 -1.213316011086981 2316 90-91 -1.3165952940239336 2316 92-93 -1.4344251656971956 2316 94-95 -1.3655151060487114 2316 96-97 -1.3628004973370587 2316 98-99 -1.7010381196560687 2316 100-101 -1.8244949753396504 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 7.0 5 7.0 6 22.0 7 64.0 8 148.0 9 266.0 10 503.0 11 914.0 12 1645.0 13 2688.0 14 3019.0 15 3977.0 16 5286.0 17 7137.0 18 6281.0 19 3230.0 20 2483.0 21 2473.0 22 2676.0 23 3049.0 24 3611.0 25 4257.0 26 5297.0 27 6539.0 28 8412.0 29 10464.0 30 13362.0 31 16424.0 32 20657.0 33 26560.0 34 35552.0 35 52935.0 36 80349.0 37 169984.0 38 309006.0 39 85293.0 40 106.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.418638118166044 22.703410308483406 12.810063705118377 23.067887868232173 2 14.791669694371375 23.792616574084114 39.517080167285805 21.898633564258702 3 16.523804467494145 30.16983631110391 31.420891151634372 21.885468069767573 4 10.120545219825972 18.6718230438646 43.021957448711 28.185674287598427 5 9.784113961897203 41.63957146928807 37.60373762832729 10.972576940487434 6 25.919066487087633 42.764995501209924 19.334290839792775 11.981647171909666 7 24.938833220630723 34.796269077943634 25.341209475960813 14.92368822546483 8 24.303637593119365 36.566053974303806 22.973560526889354 16.156747905687478 9 26.54856178431515 15.364513767415348 23.8011143586849 34.2858100895846 10-11 21.71100538793284 29.169947618406265 31.110072880439976 18.00897411322092 12-13 22.426217048458398 27.43881924923297 31.115252854356562 19.019710847952073 14-15 20.626086276175414 29.053521630518002 28.2749794620453 22.045412631261282 16-17 18.25992630888904 32.257045951040624 29.94772994381793 19.535297796252404 18-19 18.004269659153778 31.038577823479773 32.74280892157575 18.214343595790698 20-21 18.29604832985911 30.906352515131026 32.81518076194415 17.982418393065718 22-23 18.47980015312652 30.20917976718063 32.6696546829331 18.641365396759753 24-25 18.32126085112257 30.883777232943487 32.658071648006995 18.13689026792695 26-27 17.79847574487253 31.02893648080039 32.9518876586974 18.22070011562968 28-29 17.97708978708826 29.66993846310329 33.70049062974863 18.65248112005982 30-31 18.12976288675926 30.67857933245257 32.96498852100979 18.226669259778376 32-33 17.772441262157354 31.242088603173556 32.974226939518225 18.01124319515086 34-35 18.194824471985264 30.735174454995796 33.15343500187778 17.916566071141155 36-37 18.157787377680414 30.21750672024232 32.387767761837964 19.236938140239303 38-39 17.66462665288898 31.378470710592072 32.80316447101126 18.15373816550769 40-41 17.640796307949554 30.77964275455219 33.65201373900583 17.92754719849242 42-43 17.911545494784566 30.528741901879165 32.58428975735817 18.975422845978102 44-45 17.54475188759913 31.20348067132023 32.566575942681816 18.685191498398822 46-47 18.149507166831082 30.75278974035084 32.25026479128299 18.847438301535092 48-49 18.098034864814863 31.34345626583513 31.872314001315715 18.68619486803429 50-51 18.203238873637428 30.797579339885 31.648934981087194 19.350246805390373 52-53 17.841080633361408 31.308142475577718 32.052869167867826 18.797907723193052 54-55 18.595500186379173 31.1425909667295 31.664508542984294 18.597400303907033 56-57 18.570207838383567 31.38270731444239 31.065604141901716 18.98148070527233 58-59 17.791853676229373 30.938940294979115 32.27199643221801 18.9972095965735 60-61 18.510876587449733 31.963933231545795 31.062375792746856 18.462814388257623 62-63 18.345507077910103 31.5907833483293 31.97857346440367 18.085136109356924 64-65 18.33867510918739 30.760748984969442 31.701143708207706 19.19943219763546 66-67 18.14426306465404 31.49331887759379 31.094798728043948 19.26761932970822 68-69 18.092960092099453 31.103908079379895 31.5675908280568 19.23554100046385 70-71 18.591424458244475 30.435215115057385 32.27720625382817 18.696154172869974 72-73 18.429344418227483 30.906109620184402 31.10746855548017 19.557077406107943 74-75 18.156110809949872 31.08116264383554 31.059926119248676 19.702800426965915 76-77 17.83481970363684 31.07254972891428 32.092215063204804 19.000415504244078 78-79 17.466144659063318 30.768722957406553 31.86228711387904 19.90284526965109 80-81 17.90258032102142 31.43117253126972 30.702137255351243 19.96410989235762 82-83 18.76643734945819 30.705667357785142 30.781336448023605 19.746558844733062 84-85 17.75347861416527 30.647124505829453 31.188825961432066 20.410570918573214 86-87 17.846061561722035 30.922597681069565 31.395090528488062 19.83625022872034 88-89 18.35506480086937 30.427034159247157 31.331148661677787 19.88675237820568 90-91 18.06964413254294 30.197157231322258 31.702241452576978 20.030957183557824 92-93 18.562573182673482 30.873629462636686 30.92996155404826 19.63383580064157 94-95 18.27693239711803 29.649444597201636 31.2944719034813 20.779151102199034 96-97 18.621135208936096 29.8080786798612 30.98628702299803 20.584499088204673 98-99 17.932003447108016 30.048018080551003 31.25355580816464 20.766422664176343 100-101 17.87318014236399 30.042355997154285 31.062107785087044 21.02235607539468 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 494.0 1 342.5 2 379.5 3 638.0 4 552.0 5 546.0 6 1283.5 7 2146.0 8 2615.0 9 2598.0 10 2043.5 11 1555.5 12 1454.0 13 1432.0 14 1388.5 15 1400.0 16 1403.5 17 1470.0 18 1569.0 19 1688.5 20 2061.5 21 2485.0 22 2820.0 23 3459.0 24 3904.5 25 4748.5 26 6975.0 27 9488.5 28 10252.5 29 11965.5 30 17498.5 31 22128.0 32 27930.0 33 36100.5 34 42435.0 35 48111.5 36 57658.0 37 63176.5 38 58918.0 39 55248.0 40 50017.0 41 42821.5 42 35827.0 43 33605.5 44 33833.5 45 32096.0 46 27975.0 47 21746.5 48 18906.0 49 16264.0 50 13508.0 51 10478.5 52 7645.0 53 6089.0 54 5335.5 55 4775.5 56 4001.0 57 3232.0 58 2648.5 59 2265.5 60 1932.5 61 1579.0 62 1040.5 63 604.0 64 358.0 65 243.0 66 232.0 67 258.0 68 268.5 69 219.5 70 173.5 71 140.0 72 112.5 73 111.5 74 82.0 75 40.5 76 19.0 77 14.5 78 10.5 79 4.0 80 15.5 81 23.0 82 9.5 83 2.0 84 2.0 85 0.5 86 0.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27372762480649615 2 0.018777558582070787 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.588559101806782E-5 12-13 0.0 14-15 0.0 16-17 5.029703191626102E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0021795380497046444 26-27 0.002067766867668509 28-29 0.002067766867668509 30-31 7.823982742529495E-4 32-33 0.0011735974113794242 34-35 0.001453025366469763 36-37 0.0 38-39 1.6765677305420344E-4 40-41 6.706270922168137E-4 42-43 0.0036884490071924752 44-45 0.0033531354610840686 46-47 0.012574257979065258 48-49 0.012909571525173663 50-51 0.005923872647915188 52-53 0.006873927695222341 54-55 3.9119913712647473E-4 56-57 0.0026266227778491873 58-59 0.0012853685934155596 60-61 0.001229483002397492 62-63 0.0 64-65 8.382838652710172E-4 66-67 0.0 68-69 0.0 70-71 1.1177118203613563E-4 72-73 0.001564796548505899 74-75 0.0 76-77 0.2005175005728273 78-79 0.10685325002654565 80-81 0.0010618262293432884 82-83 0.0 84-85 4.4708472814454254E-4 86-87 0.12591023656370678 88-89 0.5931137774747537 90-91 0.3138534791574688 92-93 0.29658483153288584 94-95 0.0021236524586865767 96-97 0.0024030804137769157 98-99 0.002458966004794984 100-101 7.265126832348816E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 894685.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.671266469696807 #Duplication Level Percentage of deduplicated Percentage of total 1 66.43274183213911 14.396816505568607 2 13.068886316076883 5.664386356357529 3 5.794507822697684 3.7672296925927244 4 3.197507341331917 2.77176134531263 5 2.1070543305808114 2.2831267932072694 6 1.4256485912481311 1.853736630785167 7 1.000439897433309 1.5176559722935368 8 0.7517126685658523 1.3032452439309972 9 0.6361746231181871 1.2408038800967855 >10 4.324218134516199 18.745349090353773 >50 0.5986143831474723 9.240782765564541 >100 0.6042811285975032 26.020933212474183 >500 0.045849122289154116 6.8256441490220325 >1k 0.0118486495803432 3.7806246988416907 >5k 5.15158677406226E-4 0.5879036635985218 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5266 0.5885870446022903 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3710 0.41467108535406316 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2809 0.31396525033950495 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1904 0.2128123305968022 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1736 0.19403477201473143 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 1662 0.1857637045440574 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1588 0.17749263707338336 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1475 0.16486249350330004 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 1441 0.16106227331407144 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 1422 0.15893862085538485 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 1334 0.14910275683620494 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 1266 0.1415023164577477 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1257 0.14049637581942248 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1219 0.13624907090204932 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1199 0.1340136472613266 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1187 0.132672393076893 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1179 0.1317782236206039 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 1152 0.12876040170562822 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 1115 0.12462486797029121 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 1104 0.12339538496789373 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1045 0.11680088522776173 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1029 0.11501254631518355 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1029 0.11501254631518355 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 1002 0.1119947244002079 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 987 0.11031815666966585 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 977 0.1092004448493045 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 977 0.1092004448493045 No Hit CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA 958 0.10707679239061793 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 942 0.10528845347803975 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 918 0.10260594510917251 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 916 0.10238240274510024 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.1177118203613562E-4 0.0 20-21 0.0 0.0 0.0 1.1177118203613562E-4 0.0 22-23 0.0 0.0 0.0 9.500550473071528E-4 0.0 24-25 0.0 0.0 0.0 0.0022354236407227126 0.0 26-27 0.0 0.0 0.0 0.003185478688029865 0.0 28-29 0.0 0.0 0.0 0.006091529420969391 0.0 30-31 0.0 0.0 0.0 0.012965457116191733 0.0 32-33 0.0 0.0 0.0 0.025707371868311193 0.0 34-35 0.0 0.0 0.0 0.0506882310533875 0.0 36-37 0.0 0.0 0.0 0.09522904709478755 0.0 38-39 0.0 0.0 0.0 0.16452717995719163 0.0 40-41 0.0 0.0 0.0 0.2450024310232093 0.0 42-43 0.0 0.0 0.0 0.3225157457652693 0.0 44-45 0.0 0.0 0.0 0.41528582685526194 0.0 46-47 0.0 0.0 0.0 0.5283982630758312 0.0 48-49 0.0 0.0 0.0 0.6582763766018207 0.0 50-51 0.0 0.0 0.0 0.7942460195487797 0.0 52-53 0.0 0.0 0.0 0.9249065313490223 0.0 54-55 0.0 0.0 0.0 1.0733945466840284 0.0 56-57 0.0 0.0 0.0 1.2420572603765572 0.0 58-59 0.0 0.0 0.0 1.4334654096134392 0.0 60-61 0.0 0.0 0.0 1.6359389058718992 0.0 62-63 0.0 0.0 0.0 1.828632423702197 0.0 64-65 0.0 0.0 0.0 2.0532924995948294 0.0 66-67 0.0 0.0 0.0 2.312378099554592 0.0 68-69 0.0 0.0 0.0 2.5956062748341595 0.0 70-71 0.0 0.0 0.0 2.894929500326931 0.0 72-73 0.0 0.0 0.0 3.184640404164594 0.0 74-75 0.0 0.0 0.0 3.4955878325891234 0.0 76-77 0.0 0.0 0.0 3.844313920541867 0.0 78-79 0.0 0.0 0.0 4.23584837121445 0.0 80-81 0.0 0.0 0.0 4.663484913684705 0.0 82-83 0.0 0.0 0.0 5.077876571083678 0.0 84-85 0.0 0.0 0.0 5.497409702856313 0.0 86-87 0.0 0.0 0.0 5.953492011154764 0.0 88-89 0.0 0.0 0.0 6.462330317374271 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGATGC 290 0.0 47.437183 7 TGGGCGA 40 5.422799E-4 47.43718 6 GGTATCA 1865 0.0 43.118507 1 GTATCAA 3240 0.0 40.68091 1 CATTCCG 175 0.0 40.66044 9 GGATGCC 340 0.0 40.46113 8 GTACATG 4225 0.0 38.292 1 TAACGCA 50 0.0016261621 37.949745 4 CACAGCG 65 1.3383287E-4 36.490135 7 ACATGGG 4410 0.0 36.03505 3 ATGGGTA 580 0.0 35.986828 5 CATGGGT 755 0.0 35.813503 4 GGTATGC 245 0.0 34.851807 8 ATGGGAT 1280 0.0 34.095474 5 GGGTATG 390 0.0 34.057465 7 CATGGGA 2250 0.0 33.733105 4 TACATGG 4845 0.0 33.008434 2 CATGGGG 1305 0.0 31.98829 4 GTCATAC 120 1.4821853E-7 31.7223 1 TCATACG 75 3.0947776E-4 31.637165 2 >>END_MODULE