##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936364_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 683259 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06521245969684 33.0 31.0 34.0 30.0 34.0 2 32.14283456200357 33.0 31.0 34.0 30.0 34.0 3 32.19252728467536 34.0 31.0 34.0 30.0 34.0 4 35.726876630970104 37.0 35.0 37.0 35.0 37.0 5 35.7713707393536 37.0 35.0 37.0 35.0 37.0 6 35.90761043762321 37.0 36.0 37.0 35.0 37.0 7 35.86927358439479 37.0 35.0 37.0 35.0 37.0 8 35.89190775386786 37.0 36.0 37.0 35.0 37.0 9 37.58484118028449 39.0 38.0 39.0 35.0 39.0 10-11 37.54620136727068 39.0 37.5 39.0 35.0 39.0 12-13 37.50696587970302 39.0 37.0 39.0 35.0 39.0 14-15 38.70260911308889 40.0 38.0 41.0 35.0 41.0 16-17 38.70135336673209 40.0 38.0 41.0 35.5 41.0 18-19 38.80397770098894 40.0 38.5 41.0 35.5 41.0 20-21 38.7733787626654 40.0 38.5 41.0 35.0 41.0 22-23 38.61586675038309 40.0 38.0 41.0 35.0 41.0 24-25 38.74020612973997 40.0 38.0 41.0 35.0 41.0 26-27 38.63287640557973 40.0 38.0 41.0 35.0 41.0 28-29 38.500554694486276 40.0 38.0 41.0 35.0 41.0 30-31 38.295011847630256 40.0 38.0 41.0 34.5 41.0 32-33 38.00106474997036 40.0 37.0 41.0 34.0 41.0 34-35 37.68982406378841 40.0 37.0 41.0 33.5 41.0 36-37 37.44112408325393 40.0 37.0 41.0 33.0 41.0 38-39 37.22417267829623 40.0 37.0 41.0 33.0 41.0 40-41 37.03935696419659 40.0 36.5 41.0 33.0 41.0 42-43 36.775886596444394 40.0 36.0 41.0 32.0 41.0 44-45 36.45202624480614 40.0 36.0 41.0 31.0 41.0 46-47 35.9724657853025 40.0 36.0 41.0 29.5 41.0 48-49 35.6187719444603 40.0 36.0 41.0 27.0 41.0 50-51 35.21116004326324 39.5 35.5 40.5 25.5 41.0 52-53 35.246946619071245 39.5 36.0 40.5 25.0 41.0 54-55 35.36319097150568 40.0 36.0 41.0 24.0 41.0 56-57 35.25925381151218 40.0 36.0 41.0 23.0 41.0 58-59 35.08477678303542 40.0 35.5 41.0 22.5 41.0 60-61 34.853052795499224 39.5 35.0 41.0 20.5 41.0 62-63 34.582255777091845 39.0 35.0 41.0 18.0 41.0 64-65 34.29437446122188 39.0 35.0 41.0 12.0 41.0 66-67 33.94742330507172 38.5 35.0 40.0 7.5 41.0 68-69 33.55765822330917 37.5 34.5 40.0 2.0 41.0 70-71 33.14138562389958 37.0 34.0 39.5 2.0 41.0 72-73 32.70918714572366 36.5 34.0 39.0 2.0 41.0 74-75 32.262335366237394 36.0 33.5 39.0 2.0 40.0 76-77 31.852106595010092 35.5 33.0 37.5 2.0 40.0 78-79 31.418023765512054 35.0 33.0 37.0 2.0 39.0 80-81 30.970773162153737 35.0 32.0 37.0 2.0 39.0 82-83 30.555443836085583 35.0 32.0 36.0 2.0 38.5 84-85 30.16669374278275 35.0 32.0 36.0 2.0 37.0 86-87 29.848225197179985 35.0 31.0 35.5 2.0 37.0 88-89 29.476571841717416 35.0 31.0 35.0 2.0 36.5 90-91 29.106627940502797 34.5 31.0 35.0 2.0 36.0 92-93 28.809040934696796 34.0 30.5 35.0 2.0 36.0 94-95 28.44431028350889 34.0 30.0 35.0 2.0 36.0 96-97 28.060336563440803 34.0 29.0 35.0 2.0 35.0 98-99 27.68902934319197 34.0 29.0 35.0 2.0 35.0 100-101 24.686743972637025 30.0 22.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.42502657346892647 1101 2 0.29578374866621004 1101 3 0.5247265708676352 1101 4 0.3754131160300034 1101 5 0.4750779004694934 1101 6 0.42366233842525247 1101 7 0.49301303239368366 1101 8 0.5073946915335981 1101 9 0.4143782679388721 1101 10-11 0.4824181459011214 1101 12-13 0.5233261324144749 1101 14-15 0.586680236566842 1101 16-17 0.68065997003918 1101 18-19 0.6039919768443198 1101 20-21 0.8263044218033997 1101 22-23 0.8745285216377496 1101 24-25 0.7145192308581585 1101 26-27 0.6350743775239351 1101 28-29 0.6075043311104693 1101 30-31 0.6234600480495516 1101 32-33 0.7179274955969461 1101 34-35 0.7973520454423095 1101 36-37 0.5571794974321023 1101 38-39 0.7442408509875875 1101 40-41 0.7190917821569016 1101 42-43 0.8464461714592346 1101 44-45 0.9685286493882117 1101 46-47 0.9799489032736872 1101 48-49 0.9346893047911351 1101 50-51 1.2194866076966804 1101 52-53 1.296429438273286 1101 54-55 1.3039602699760735 1101 56-57 1.2208782791677564 1101 58-59 1.3131529628980303 1101 60-61 1.341731622608819 1101 62-63 1.365671591661446 1101 64-65 1.312448560619309 1101 66-67 1.2931101243189431 1101 68-69 1.2584528757353937 1101 70-71 1.1445660627315846 1101 72-73 1.3786315481924234 1101 74-75 1.2487243698818489 1101 76-77 1.0659241309490817 1101 78-79 1.2065461666058042 1101 80-81 1.0862303008758616 1101 82-83 1.2421484790041077 1101 84-85 1.0994930987262457 1101 86-87 1.1620553513952423 1101 88-89 1.1663202068147385 1101 90-91 1.1426451733620162 1101 92-93 1.0394896960162967 1101 94-95 1.201381371962313 1101 96-97 1.2317555240537246 1101 98-99 1.1047108229957843 1101 100-101 1.1586656949183478 1102 1 0.3472810250620384 1102 2 0.3379580811668319 1102 3 0.3116123404645421 1102 4 0.2781137811334773 1102 5 0.3961375936657632 1102 6 0.3047934615620349 1102 7 0.27424446412314296 1102 8 0.19396867391250794 1102 9 0.25579166073326576 1102 10-11 0.2789322625785502 1102 12-13 0.3856446026126008 1102 14-15 0.3612334799844845 1102 16-17 0.3315988456884682 1102 18-19 0.2396226112162978 1102 20-21 0.3205782067596701 1102 22-23 0.19184868494269125 1102 24-25 0.29280305945186313 1102 26-27 0.31647480048118837 1102 28-29 0.21023917780443213 1102 30-31 0.23589511900031113 1102 32-33 0.07950642951466591 1102 34-35 0.05308464956837611 1102 36-37 0.2692379319791556 1102 38-39 -0.00350540021835144 1102 40-41 0.053991505509642934 1102 42-43 0.2510571721811914 1102 44-45 0.11246509079713718 1102 46-47 0.33081334611376434 1102 48-49 0.24739655064941246 1102 50-51 0.22733399807599852 1102 52-53 0.27119416031624866 1102 54-55 0.13193103353530944 1102 56-57 0.2088731934582171 1102 58-59 0.16313251783455485 1102 60-61 0.22827749302545897 1102 62-63 0.29336794413433864 1102 64-65 0.02079973014082981 1102 66-67 -0.025505425429642514 1102 68-69 -0.0777693876609149 1102 70-71 0.05242750909204119 1102 72-73 -0.0025874248264798894 1102 74-75 0.15476492777651885 1102 76-77 -0.06648640592550592 1102 78-79 -0.00319622559440802 1102 80-81 -0.12032393923863083 1102 82-83 -0.012865353359153175 1102 84-85 0.016291274004331058 1102 86-87 -0.0930639008265608 1102 88-89 -0.4120850143433472 1102 90-91 -0.5853428407195231 1102 92-93 -0.3121685903040401 1102 94-95 -0.2490457381918567 1102 96-97 0.18038251025969387 1102 98-99 0.047847990204459734 1102 100-101 0.10714413496000574 1103 1 0.12058186211281452 1103 2 0.10378435553904097 1103 3 0.20378566510992613 1103 4 0.06115097225171695 1103 5 -0.03900976736877482 1103 6 0.113856917027924 1103 7 0.2442710292076029 1103 8 0.10119763486684974 1103 9 0.0705816461976525 1103 10-11 0.1192316210066906 1103 12-13 0.21024612634574424 1103 14-15 0.3397746558653907 1103 16-17 0.4019994521743584 1103 18-19 0.13126964645262973 1103 20-21 0.31031869595820183 1103 22-23 0.3404345954233676 1103 24-25 0.33157931126881124 1103 26-27 0.26374248129918954 1103 28-29 0.035006733315434246 1103 30-31 0.14795779756278904 1103 32-33 0.26309685104742186 1103 34-35 0.06211928442377257 1103 36-37 0.21877993488627112 1103 38-39 0.2248509478343763 1103 40-41 0.1347080109479677 1103 42-43 0.18986349767866528 1103 44-45 0.4998015651593235 1103 46-47 0.5588376306107747 1103 48-49 0.6227724716558782 1103 50-51 0.6179065257688521 1103 52-53 0.5904419423823128 1103 54-55 0.6453388827659268 1103 56-57 0.6301334059788886 1103 58-59 0.5383549377633869 1103 60-61 0.3896635137762985 1103 62-63 0.29208668003730054 1103 64-65 0.2305918458242573 1103 66-67 0.5449828959577516 1103 68-69 0.44981694886357104 1103 70-71 0.29963629561903105 1103 72-73 0.25778799495197546 1103 74-75 0.4332816687920058 1103 76-77 0.41748682849883423 1103 78-79 0.23132522696436197 1103 80-81 0.42639990919001747 1103 82-83 0.42524865095140996 1103 84-85 0.3189108168843866 1103 86-87 0.3560175988476395 1103 88-89 0.4289254613220308 1103 90-91 0.34232055961128793 1103 92-93 0.414154651638718 1103 94-95 0.295557289225318 1103 96-97 0.39968229473165096 1103 98-99 0.5355115158422734 1103 100-101 0.7442268626428294 1104 1 0.012463416780789771 1104 2 -0.1837217757762133 1104 3 -0.11986019012095994 1104 4 -0.17728419930832473 1104 5 -0.10627029571296731 1104 6 0.09099295047570877 1104 7 0.09679245961425664 1104 8 -0.11737791461578695 1104 9 -0.09666019623338684 1104 10-11 -0.061202768080732994 1104 12-13 -0.03549406938919475 1104 14-15 0.16437524447165686 1104 16-17 0.15829572900616284 1104 18-19 0.11985372138313721 1104 20-21 0.0110360598430006 1104 22-23 0.15226015074535582 1104 24-25 0.1721621949723655 1104 26-27 0.12713531758723917 1104 28-29 0.28077174627878776 1104 30-31 0.22252189024491287 1104 32-33 0.2404941953120101 1104 34-35 0.1828244440715494 1104 36-37 0.15175152061958386 1104 38-39 0.04167498492591193 1104 40-41 0.21920045483183515 1104 42-43 0.14130640053527088 1104 44-45 0.17832794733296709 1104 46-47 0.4278917146506771 1104 48-49 0.0043767740873832395 1104 50-51 -0.26695759260640983 1104 52-53 -0.06638396330053808 1104 54-55 -0.06811263896813102 1104 56-57 -0.21821143086972938 1104 58-59 -0.15329099921837042 1104 60-61 -0.40968335516538446 1104 62-63 -0.39260167396732726 1104 64-65 -0.27932774635569757 1104 66-67 -0.2282328913031222 1104 68-69 -0.3228134437741801 1104 70-71 -0.2234159899017243 1104 72-73 -0.10454353986222031 1104 74-75 -0.15725887963954222 1104 76-77 -0.04969019717093559 1104 78-79 0.08871521365065149 1104 80-81 0.16936787452765145 1104 82-83 0.10330350578522385 1104 84-85 0.08674564509077598 1104 86-87 0.11650214793320046 1104 88-89 0.08541414466587582 1104 90-91 0.2786497703650319 1104 92-93 0.06003032024404931 1104 94-95 -0.05315980604948223 1104 96-97 0.3109942794092042 1104 98-99 0.3600945864049514 1104 100-101 0.3857186408410378 1105 1 0.1183597056445933 1105 2 0.1555064528286607 1105 3 0.16564505675390961 1105 4 0.013757789626552608 1105 5 0.017313466149985857 1105 6 0.037590494966138976 1105 7 0.11014864786069722 1105 8 0.1841926638643656 1105 9 0.20773101298662056 1105 10-11 0.24505864195592153 1105 12-13 0.22019109024680006 1105 14-15 0.18007824208861223 1105 16-17 0.06719799638077717 1105 18-19 0.04971561638989641 1105 20-21 0.014610623997022287 1105 22-23 0.14719723005468666 1105 24-25 0.04471400176472429 1105 26-27 0.22952393472562704 1105 28-29 0.2313716877479166 1105 30-31 0.16872504812498335 1105 32-33 0.07704672677235891 1105 34-35 -0.03729484742615341 1105 36-37 0.2219593052259654 1105 38-39 0.42193196333916916 1105 40-41 0.45929967860094223 1105 42-43 0.46505878706202 1105 44-45 0.2318586133307079 1105 46-47 0.031397992783816164 1105 48-49 0.17205936951360457 1105 50-51 0.23010471490365347 1105 52-53 0.10620268329839888 1105 54-55 0.11745392596630211 1105 56-57 0.3849520235667754 1105 58-59 0.40547678650288077 1105 60-61 0.3029328355895302 1105 62-63 0.27151471885846234 1105 64-65 0.10675026693918 1105 66-67 -0.006672625405720112 1105 68-69 0.28387719267940525 1105 70-71 0.31172204541190496 1105 72-73 0.18256015733805242 1105 74-75 0.2498679511922859 1105 76-77 0.40314785347220194 1105 78-79 0.2609130480082733 1105 80-81 0.31491186326742593 1105 82-83 0.2962407801974756 1105 84-85 0.346767663848528 1105 86-87 0.2992564436813474 1105 88-89 0.19474981923421097 1105 90-91 0.16721255866442775 1105 92-93 0.07696385982903564 1105 94-95 0.3153909973564559 1105 96-97 -0.03080652051275834 1105 98-99 0.07635362733475404 1105 100-101 0.2541969774493147 1106 1 -0.04869964974312779 1106 2 -0.07996051877010757 1106 3 -0.06475596441405429 1106 4 0.0032083799892816955 1106 5 -0.08736954629549842 1106 6 -0.05745258555720767 1106 7 -0.04056138298708589 1106 8 -0.16377829667175092 1106 9 0.007914504104583386 1106 10-11 -0.017401468670612985 1106 12-13 -0.051286678075719294 1106 14-15 -0.02331368430219527 1106 16-17 0.21454402339917777 1106 18-19 0.10931831823013027 1106 20-21 0.03157025595106688 1106 22-23 -0.0890648697219305 1106 24-25 -0.11391008077968223 1106 26-27 0.06340596855181957 1106 28-29 0.08543907419616659 1106 30-31 -0.10386962265472022 1106 32-33 0.03376675655643169 1106 34-35 -0.25672097230648205 1106 36-37 -0.23925625694828057 1106 38-39 -0.2652542402561906 1106 40-41 -0.3668185535020285 1106 42-43 -0.34046722080947944 1106 44-45 -0.19225584257824124 1106 46-47 -0.029090253147465717 1106 48-49 -0.0619529695992469 1106 50-51 -0.17425785290827633 1106 52-53 -0.1691297281471904 1106 54-55 0.09635510669175318 1106 56-57 0.18574715174462852 1106 58-59 0.17838658333300828 1106 60-61 0.17651277389396114 1106 62-63 -0.11616345367637138 1106 64-65 0.003746597116823125 1106 66-67 0.03293752245088655 1106 68-69 0.32593122948399866 1106 70-71 0.5318635197231458 1106 72-73 0.4986063226396098 1106 74-75 0.5547716848376467 1106 76-77 0.5363025710554403 1106 78-79 0.47533039722643977 1106 80-81 0.4837742183360376 1106 82-83 0.4861886580828063 1106 84-85 0.35599407593234744 1106 86-87 0.28680962951264277 1106 88-89 0.30642144991179876 1106 90-91 0.4283430235085959 1106 92-93 0.321959073104221 1106 94-95 0.2526463425325005 1106 96-97 0.27040345714919667 1106 98-99 -0.0714817093315574 1106 100-101 0.35118665939804217 1107 1 -0.11357306746464957 1107 2 0.09960154052816961 1107 3 0.1314253285941902 1107 4 0.03930344412996334 1107 5 0.041113590226458996 1107 6 0.11984755886552989 1107 7 0.13027159636895647 1107 8 0.06488749213124123 1107 9 0.11910938984071606 1107 10-11 0.17906123628223725 1107 12-13 0.173593323623372 1107 14-15 0.23136412556952024 1107 16-17 0.014645968858353342 1107 18-19 0.18578803193329918 1107 20-21 0.17512751531730686 1107 22-23 0.12319407174438624 1107 24-25 0.16664169901812897 1107 26-27 0.09126044140884204 1107 28-29 0.18057224045221432 1107 30-31 0.12424927123704776 1107 32-33 0.29748691627097656 1107 34-35 -0.017531438706953395 1107 36-37 0.1209032452180665 1107 38-39 0.09773000067490756 1107 40-41 -0.03079676266012399 1107 42-43 -0.10933775007632107 1107 44-45 -0.12516298725584107 1107 46-47 -0.10973335908578719 1107 48-49 -0.28413035469822034 1107 50-51 -0.31692308950669457 1107 52-53 -0.19152587121065068 1107 54-55 -0.31467624153694373 1107 56-57 -0.3402383210274422 1107 58-59 -0.373977277001714 1107 60-61 -0.5271232808167667 1107 62-63 -0.3944798668953027 1107 64-65 -0.4997524683831429 1107 66-67 -0.4026669291674949 1107 68-69 -0.3434999998083228 1107 70-71 -0.26298186299709414 1107 72-73 -0.41132061644797346 1107 74-75 -0.3299343724217181 1107 76-77 -0.24923497885535895 1107 78-79 -0.3577604616640535 1107 80-81 -0.3358354155098553 1107 82-83 -0.2052988960757176 1107 84-85 -0.24232488592306467 1107 86-87 -0.4586506117582765 1107 88-89 -0.06236624866946272 1107 90-91 -0.035556602691386274 1107 92-93 -0.28539161927535517 1107 94-95 -0.30179359460113275 1107 96-97 -0.29861149485148175 1107 98-99 -0.011503797514379244 1107 100-101 0.2842530938556074 1108 1 0.12101339870076089 1108 2 0.20459977436780719 1108 3 0.08647654724484255 1108 4 0.20287764809625486 1108 5 0.1856460623463576 1108 6 0.109417120353136 1108 7 0.020719191867776487 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-0.7676526466198865 2316 58-59 -0.7558928498472355 2316 60-61 -0.8098488257475722 2316 62-63 -0.9289575054294801 2316 64-65 -0.6841454718873266 2316 66-67 -0.8660697028207522 2316 68-69 -0.9089251052309066 2316 70-71 -0.9026259504235981 2316 72-73 -0.9517969483360744 2316 74-75 -0.933998338686493 2316 76-77 -1.2416883553476055 2316 78-79 -1.2588748228923023 2316 80-81 -1.2400901817983865 2316 82-83 -0.9881098184490611 2316 84-85 -1.1008433274506508 2316 86-87 -1.1699857664770512 2316 88-89 -1.2649001026814588 2316 90-91 -1.3640704412612017 2316 92-93 -1.7906627483363522 2316 94-95 -1.6521789067113666 2316 96-97 -1.5319517573685602 2316 98-99 -1.577047688943967 2316 100-101 -1.800277998381727 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3350.0 3 494.0 4 358.0 5 308.0 6 369.0 7 423.0 8 499.0 9 754.0 10 999.0 11 1604.0 12 2967.0 13 4972.0 14 5244.0 15 5811.0 16 7506.0 17 10829.0 18 8904.0 19 4151.0 20 3379.0 21 3167.0 22 3153.0 23 3469.0 24 3643.0 25 3979.0 26 4291.0 27 4956.0 28 6099.0 29 7567.0 30 9366.0 31 11353.0 32 15195.0 33 20230.0 34 28806.0 35 46423.0 36 67508.0 37 133497.0 38 199785.0 39 47840.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.8558669491513 22.803215873801406 13.681812391743915 20.65910478530338 2 14.1859511995199 24.094322223685943 40.57070507472299 21.149021502071168 3 17.610965961900163 31.110135318096326 31.856324236502953 19.42257448350056 4 9.973551981991344 18.713107063977272 43.17247415573448 28.140866798296905 5 8.925380081351388 43.08359521025045 36.80089108896399 11.19013361943417 6 24.497672784754545 43.05992213342701 18.938116565674306 13.504288516144141 7 23.784746616083556 37.33665870451033 24.481728022106694 14.396866657299423 8 24.097785740213816 35.616093488366644 22.44499478928818 17.841125982131356 9 26.527442737364748 16.76747154433463 24.492804112604805 32.21228160569582 10-11 21.59367080548528 29.423787754492704 30.2496975835222 18.732843856499816 12-13 23.74452582522453 28.515913162609337 30.060537932811094 17.679023079355044 14-15 21.03924275002232 29.926274390413475 29.574771409627214 19.45971144993699 16-17 16.98993561457451 33.09434290929611 31.12867350799932 18.787047968130054 18-19 16.81001296849797 31.404915734037473 34.677119529738256 17.1079517677263 20-21 17.56234106002671 30.507839514078196 34.52752520170512 17.40229422418998 22-23 17.90069562099085 30.324537746861292 33.76553803594103 18.009228596206835 24-25 18.151463229153478 31.039063603606635 33.76804837150203 17.04142479573785 26-27 17.137968484252372 30.50764474966848 35.367615417716856 16.9867713483623 28-29 16.769791291568225 30.528036138264582 35.773998249296646 16.92817432087055 30-31 17.32697180346298 30.802407603389497 34.90680929608925 16.963811297058268 32-33 16.568353074117226 31.47120558323624 35.33302692670246 16.627414415944074 34-35 17.280456167535334 30.669519181105322 34.80229022867071 17.24773442268864 36-37 17.692794024671755 30.025950921147427 34.159204857894814 18.122050196286004 38-39 16.789321359706484 31.59323190154103 34.42616202181394 17.19128471693855 40-41 16.44209092927739 31.326440429220774 35.56504706458458 16.66642157691725 42-43 16.52794738347323 31.279734094212465 33.89004845678627 18.30227006552804 44-45 16.89275685421282 30.978534606173664 33.748969671390434 18.379738868223086 46-47 18.092560741285986 30.99910508586249 33.07343811010605 17.834896062745468 48-49 17.61415238850236 31.417746522167896 32.78946513049086 18.178635958838882 50-51 17.281700634192873 31.385817702828827 32.997887004378235 18.334594658600068 52-53 16.932902884205262 32.31883631657131 32.82530040740686 17.922960391816563 54-55 17.66044556078924 31.5144037400165 32.3290396869398 18.496111012254467 56-57 18.415129909614347 32.13377929372285 31.031990476897725 18.419100319765068 58-59 17.752663094306772 30.951596680175758 32.323950496738405 18.971789728779065 60-61 18.118589350059075 31.940264928805075 31.984010457754685 17.95713526338117 62-63 17.760915531271046 31.92985887078987 32.36002505565473 17.94920054228436 64-65 17.850152136156343 31.79403014487127 32.107787988817364 18.248029730155018 66-67 17.854711624275698 32.085226946923925 30.969348822369614 19.090712606430763 68-69 17.669961881007566 31.397872866101157 31.99701165923503 18.935153593656246 70-71 18.48609139384547 31.208916917426176 32.00659314321928 18.298398545509084 72-73 18.34825016156936 30.59531783394861 31.87081509627622 19.185616908205812 74-75 17.989286095086825 32.01714813058169 31.03149829217267 18.96206748215881 76-77 17.680322926929694 31.14590532770613 32.133288237283644 19.040483508080523 78-79 17.61440462684449 30.851070869511194 32.028295748064615 19.506228755579706 80-81 18.392307262454818 31.458211400286462 31.023517024549353 19.12596431270937 82-83 18.613489958596166 30.975964306381016 31.172505678229495 19.238040056793324 84-85 17.915906280191816 30.430115274834673 31.57933434995963 20.074644095013873 86-87 18.296843566546276 31.095957694615358 31.428527311443393 19.17867142739497 88-89 18.596094886994994 30.37048639990822 31.69766003557858 19.335758677518207 90-91 18.47433515313876 29.885780361107066 32.00856794406121 19.631316541692964 92-93 19.18228146574771 30.641534912011153 31.45614973144041 18.72003389080072 94-95 18.69012579786315 29.649737985454827 31.96033349043667 19.69980272624535 96-97 18.919892412651436 29.485519886678972 31.539794307926517 20.054793392743072 98-99 18.718808111544767 30.068649717389544 31.014090097241514 20.19845207382417 100-101 18.82534548662158 29.815936489267862 31.08328432813878 20.275433695971774 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2233.0 1 1341.5 2 797.0 3 1102.5 4 750.0 5 761.5 6 1659.5 7 2544.0 8 3021.5 9 2925.5 10 2180.5 11 1652.5 12 1682.5 13 1754.5 14 1775.0 15 1778.0 16 1831.0 17 1869.0 18 1836.0 19 1985.5 20 2208.5 21 2476.5 22 2943.5 23 3466.5 24 3900.5 25 4899.0 26 7328.5 27 9880.0 28 10470.5 29 11604.5 30 14916.5 31 17914.0 32 21953.0 33 26705.5 34 31052.0 35 35749.0 36 43107.0 37 48970.5 38 44759.5 39 37846.0 40 32501.0 41 26707.0 42 21772.0 43 21797.0 44 23202.5 45 21259.5 46 17196.0 47 15883.5 48 23148.5 49 21680.0 50 12567.0 51 7844.0 52 4684.0 53 3405.5 54 2590.0 55 1998.5 56 1547.0 57 1295.5 58 1144.5 59 1037.5 60 908.5 61 779.0 62 564.0 63 314.0 64 199.5 65 157.5 66 103.5 67 57.0 68 49.5 69 44.0 70 44.0 71 40.5 72 28.5 73 33.0 74 38.0 75 26.0 76 10.0 77 6.0 78 6.5 79 6.0 80 5.5 81 4.0 82 4.5 83 5.0 84 5.5 85 5.5 86 4.5 87 3.0 88 3.0 89 2.5 90 1.0 91 2.5 92 3.0 93 3.5 94 5.0 95 4.0 96 2.5 97 1.0 98 0.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03980920851390175 2 0.010098659512717725 3 0.007903298749083437 4 0.004683436295753148 5 0.007317869212114293 6 0.005707937985449149 7 0.003951649374541719 8 0.00746422659635658 9 0.013318521966048014 10-11 0.0018294673030285734 12-13 0.00746422659635658 14-15 0.007317869212114293 16-17 0.015733418806045733 18-19 0.00951322997574858 20-21 0.003146683761209146 22-23 0.008269192209689151 24-25 0.01895328125937602 26-27 0.0065860822909028635 28-29 0.015367525345440017 30-31 0.013172164581805727 32-33 0.010391374281202298 34-35 0.2568572093452117 36-37 0.4579522552941125 38-39 0.4533419976904805 40-41 0.47361249540803707 42-43 0.46373337197168274 44-45 0.4767591791692462 46-47 0.4837843336128759 48-49 0.4896386289825674 50-51 0.5003227180322543 52-53 0.4901508798274154 54-55 0.4774177873983365 56-57 0.47251481502621995 58-59 0.47170984941288735 60-61 0.46680687704077084 62-63 0.4643919802007731 64-65 0.47946679077772847 66-67 0.4569277536044165 68-69 0.4804912924674245 70-71 0.46226979812925995 72-73 0.46988038210985883 74-75 0.4831989040759068 76-77 0.47258799371834104 78-79 0.4723684576419776 80-81 0.4743442823292485 82-83 0.47558832009530794 84-85 0.4730270658710679 86-87 0.4758078561716714 88-89 0.49110220282499023 90-91 0.5145925629958771 92-93 0.5017862918746772 94-95 0.4741247462528851 96-97 0.44895127616321184 98-99 0.4381940084214038 100-101 0.4477072383971525 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 683259.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.271651287880545 #Duplication Level Percentage of deduplicated Percentage of total 1 71.38722722863406 13.757497495589268 2 10.150815444223378 3.912459510574104 3 4.467611009268088 2.58294724381532 4 2.5221100387942954 1.9442090070922613 5 1.6626694223404987 1.6021192657183931 6 1.2152579924667382 1.4052016953377242 7 0.8971067430171685 1.2102109823603195 8 0.7049188931786928 1.0867960876462788 9 0.6047120911391735 1.048842049499927 >10 4.79807776427608 19.174141064642864 >50 0.8004369967342335 10.935462511478233 >100 0.7101507383348271 26.62600158848372 >500 0.062214060409675025 7.994628743683585 >1k 0.015174161075530494 4.866200399044084 >5k 0.0015174161075530493 1.8532823550339164 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7314 1.070457908348079 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5361 0.7846219369228946 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3827 0.5601097094952281 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3653 0.5346435246370702 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2689 0.3935550062275067 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1992 0.29154390941063346 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1972 0.28861676172578776 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1737 0.25422277642885055 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1644 0.24061153969431795 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1571 0.2299274506446311 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 1369 0.20036325902768934 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1357 0.1986069704167819 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1308 0.19143545858890992 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1255 0.18367851722406878 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1236 0.1808977269234653 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1167 0.17079906741074763 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1133 0.1658229163465099 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1122 0.16421298511984475 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1091 0.15967590620833388 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1084 0.15865140451863788 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1051 0.15382161083864243 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1023 0.14972360407985844 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 991 0.14504016778410528 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 959 0.14035673148835215 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 945 0.13830772810896014 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 942 0.1378686559562333 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 918 0.13435607873441843 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 876 0.12820906859624243 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 875 0.12806271121200014 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 849 0.12425741922170071 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 835 0.1222084158423087 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 832 0.12176934368958185 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 830 0.12147662892109726 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 809 0.11840312385200927 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 808 0.11825676646776698 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 802 0.11737862216231326 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 799 0.11693955000958642 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 799 0.11693955000958642 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 788 0.11532961878292128 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 785 0.1148905466301944 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 776 0.11357333017201383 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 752 0.11006075295019897 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 750 0.1097680381817144 No Hit CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG 732 0.10713360526535326 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 726 0.10625546095989954 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 720 0.10537731665444582 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 711 0.10406010019626524 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 708 0.1036210280435384 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 707 0.10347467065929611 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 706 0.10332831327505383 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 699 0.10230381158535783 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 696 0.10186473943263095 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 691 0.10113295251141954 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.4635738424228587E-4 0.0 14-15 0.0 0.0 0.0 7.317869212114293E-4 0.0 16-17 0.0 0.0 0.0 0.002195360763634288 0.0 18-19 0.0 0.0 0.0 0.0045370789115108615 0.0 20-21 0.0 0.0 0.0 0.008561906978173724 0.0 22-23 0.0 0.0 0.0 0.016392027035136016 0.0 24-25 0.0 0.0 0.0 0.027295652161186314 0.0 26-27 0.0 0.0 0.0 0.03417444922057375 0.0 28-29 0.0 0.0 0.0 0.044126751349049184 0.0 30-31 0.0 0.0 0.0 0.07032472312841836 0.0 32-33 0.0 0.0 0.0 0.11554915485928469 0.0 34-35 0.0 0.0 0.0 0.18982552736224478 0.0 36-37 0.0 0.0 0.0 0.2977640982409306 0.0 38-39 0.0 0.0 0.0 0.4397307609559479 0.0 40-41 0.0 0.0 0.0 0.6048950690733674 0.0 42-43 0.0 0.0 0.0 0.7534478140792876 0.0 44-45 0.0 0.0 0.0 0.9199293386548879 0.0 46-47 0.0 0.0 0.0 1.1255614635152995 0.0 48-49 0.0 0.0 0.0 1.34136542658055 0.0 50-51 0.0 7.317869212114293E-5 0.0 1.5614137537888269 0.0 52-53 0.0 1.4635738424228587E-4 0.0 1.7633137653510602 0.0 54-55 0.0 1.4635738424228587E-4 0.0 1.973117075662377 0.0 56-57 0.0 1.4635738424228587E-4 0.0 2.2360481164536434 0.0 58-59 0.0 1.4635738424228587E-4 0.0 2.5067360986097516 0.0 60-61 0.0 1.4635738424228587E-4 0.0 2.813281639905219 0.0 62-63 0.0 1.4635738424228587E-4 0.0 3.086164982824961 0.0 64-65 0.0 1.4635738424228587E-4 0.0 3.3641708341931826 0.0 66-67 0.0 1.4635738424228587E-4 0.0 3.7059153263989204 0.0 68-69 0.0 1.4635738424228587E-4 0.0 4.081322016980383 0.0 70-71 0.0 1.4635738424228587E-4 0.0 4.484243895799397 0.0 72-73 0.0 1.4635738424228587E-4 0.0 4.87245685750206 0.0 74-75 0.0 1.4635738424228587E-4 0.0 5.268499939261686 0.0 76-77 0.0 1.4635738424228587E-4 0.0 5.69205820925886 0.0 78-79 0.0 1.4635738424228587E-4 0.0 6.213602748006247 0.0 80-81 0.0 1.4635738424228587E-4 0.0 6.758198574771792 0.0 82-83 0.0 1.4635738424228587E-4 0.0 7.273230209920396 0.0 84-85 0.0 1.4635738424228587E-4 0.0 7.819948218757455 0.0 86-87 0.0 1.4635738424228587E-4 0.0 8.400621140738725 0.0 88-89 0.0 1.4635738424228587E-4 0.0 9.024103597610862 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGATC 15 6.7019457E-4 94.69478 8 GTTCACG 25 0.0050818147 56.825184 1 GTCTAGA 35 2.829744E-4 54.11922 1 GACGTGG 55 8.3738087E-7 51.64792 7 GTACAGG 100 1.2732926E-11 47.354317 1 GCACCCC 65 2.6457637E-6 43.705276 8 GGATAGC 55 5.05193E-5 43.04938 1 CATGGGT 390 0.0 35.20703 4 CTCTAGG 70 2.0898313E-4 33.824512 1 CGTGGAA 85 1.6675614E-5 33.421684 9 ATGGGTA 375 0.0 32.827522 5 ATGGGAC 295 0.0 32.099922 5 ACGATAA 30 0.003927676 31.705326 42-43 GTAGTAA 75 3.1330885E-4 31.569544 1 ACGTGGA 90 2.4651676E-5 31.564924 8 GTAAGTA 75 3.1357803E-4 31.564924 4 ACGCCGA 75 3.1371266E-4 31.562613 6 GTACATG 5370 0.0 31.12863 1 CATTCCG 220 0.0 30.130157 9 ATGGGAG 410 0.0 30.025171 5 >>END_MODULE