##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936364_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 683259 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26003609173095 33.0 31.0 34.0 30.0 34.0 2 32.38675816930329 34.0 31.0 34.0 30.0 34.0 3 32.46292255206298 34.0 31.0 34.0 30.0 34.0 4 36.0127784632182 37.0 35.0 37.0 35.0 37.0 5 36.007395438625764 37.0 35.0 37.0 35.0 37.0 6 36.07012421351201 37.0 35.0 37.0 35.0 37.0 7 36.0133960913797 37.0 35.0 37.0 35.0 37.0 8 36.012796026104304 37.0 35.0 37.0 35.0 37.0 9 37.7100338231915 39.0 37.0 39.0 35.0 39.0 10-11 37.65572352504687 39.0 37.0 39.0 35.0 39.0 12-13 37.64180274244467 39.0 37.0 39.0 35.0 39.0 14-15 38.799982144399124 40.0 38.0 41.0 34.5 41.0 16-17 38.74829530236704 40.0 38.0 41.0 34.5 41.0 18-19 38.91847308268168 40.0 38.0 41.0 35.0 41.0 20-21 38.90939380235021 40.0 38.0 41.0 35.0 41.0 22-23 38.772666733991066 40.0 38.0 41.0 34.5 41.0 24-25 38.880887774621335 40.0 38.0 41.0 35.0 41.0 26-27 38.714224766889274 40.0 38.0 41.0 34.5 41.0 28-29 38.58980781811876 40.0 38.0 41.0 34.0 41.0 30-31 38.39195019750929 40.0 37.5 41.0 34.0 41.0 32-33 38.09976890169028 40.0 37.0 41.0 34.0 41.0 34-35 37.87904806230141 40.0 37.0 41.0 33.0 41.0 36-37 37.65873556001458 40.0 36.5 41.0 33.0 41.0 38-39 37.43299393055928 40.0 36.0 41.0 33.0 41.0 40-41 37.26685854119741 40.0 36.0 41.0 33.0 41.0 42-43 37.12643960782076 40.0 36.0 41.0 33.0 41.0 44-45 36.803539360623134 40.0 36.0 41.0 31.5 41.0 46-47 36.35634217771006 40.0 36.0 41.0 30.5 41.0 48-49 35.948745644038354 40.0 36.0 41.0 28.5 41.0 50-51 35.69966806145254 40.0 36.0 41.0 27.0 41.0 52-53 35.51225816271721 40.0 35.5 41.0 26.0 41.0 54-55 35.350701564121366 40.0 35.0 41.0 25.5 41.0 56-57 35.12900964348805 39.5 35.0 41.0 24.0 41.0 58-59 34.915840845126084 39.0 35.0 41.0 23.0 41.0 60-61 34.67955050720151 39.0 35.0 41.0 22.0 41.0 62-63 34.41083834973268 39.0 35.0 40.0 21.5 41.0 64-65 34.05643540736383 38.0 34.5 40.0 19.5 41.0 66-67 33.7112932284829 38.0 34.0 40.0 16.5 41.0 68-69 33.341998422267395 37.0 34.0 40.0 13.0 41.0 70-71 32.95737633898712 37.0 34.0 39.0 8.0 41.0 72-73 32.51694891688218 36.0 33.0 39.0 7.0 40.5 74-75 32.05837098377043 35.5 33.0 38.5 2.0 40.0 76-77 31.28792449129832 35.0 31.5 37.0 2.0 39.0 78-79 31.29648713006342 35.0 32.0 37.0 2.0 39.0 80-81 31.06206284878794 35.0 32.0 36.5 2.0 39.0 82-83 30.710134077999705 35.0 32.0 36.0 2.0 38.5 84-85 30.34279460643768 35.0 31.5 36.0 2.0 37.0 86-87 29.93686947409401 35.0 31.0 35.0 2.0 37.0 88-89 29.381720548137675 34.5 30.5 35.0 2.0 36.0 90-91 29.11453709354725 34.0 30.0 35.0 2.0 36.0 92-93 28.814163443145276 34.0 30.0 35.0 2.0 36.0 94-95 28.638878521907507 34.0 30.0 35.0 2.0 35.5 96-97 28.36025357880394 34.0 30.0 35.0 2.0 35.0 98-99 27.962580075783855 34.0 29.0 35.0 2.0 35.0 100-101 24.895807446370995 30.0 23.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34108828557324244 1101 2 0.3273047751289653 1101 3 0.3308041632590246 1101 4 0.40945321906248466 1101 5 0.25855218769750365 1101 6 0.38288152469991843 1101 7 0.29719708585101756 1101 8 0.293517543467523 1101 9 0.41985012568304114 1101 10-11 0.3743090962922935 1101 12-13 0.44406683860022866 1101 14-15 0.6204137113938586 1101 16-17 0.5323354088868868 1101 18-19 0.5755971384970024 1101 20-21 0.5255550239773967 1101 22-23 0.7366793124775697 1101 24-25 0.6087183349142009 1101 26-27 0.7623659425835072 1101 28-29 0.7707865150287034 1101 30-31 0.7080996577163816 1101 32-33 0.8409496275573005 1101 34-35 0.7067587987814079 1101 36-37 0.2872264774893907 1101 38-39 0.377383905168152 1101 40-41 0.45909944315364015 1101 42-43 0.3535357133529331 1101 44-45 0.16451704112386523 1101 46-47 0.25059845214785526 1101 48-49 0.5092469267922439 1101 50-51 0.6495972904759597 1101 52-53 0.5722039411359887 1101 54-55 0.5098263651192738 1101 56-57 0.484911058693136 1101 58-59 0.5859445188159356 1101 60-61 0.7832610363829318 1101 62-63 0.5600529620626773 1101 64-65 0.8562921085202291 1101 66-67 0.7440657056608941 1101 68-69 0.9279318004486043 1101 70-71 0.8906812638750523 1101 72-73 1.0344917441110368 1101 74-75 1.1361906922476663 1101 76-77 0.8623214800098502 1101 78-79 0.8048081223942454 1101 80-81 1.0536809606655417 1101 82-83 0.9274599869234095 1101 84-85 0.997684113020906 1101 86-87 1.003626669411947 1101 88-89 1.001772711625886 1101 90-91 1.4788677864582276 1101 92-93 1.5350522866253336 1101 94-95 1.4290875557749025 1101 96-97 1.2072131645258288 1101 98-99 1.3456428242020024 1101 100-101 1.6299044565699283 1102 1 0.09166170571290166 1102 2 0.10888441184574305 1102 3 0.18109199141826338 1102 4 0.04962010189310462 1102 5 0.02461593796105177 1102 6 0.06716502106133504 1102 7 0.059334745019029356 1102 8 0.1515968575356439 1102 9 0.04992545296378381 1102 10-11 0.15739815371097876 1102 12-13 0.2855233577003915 1102 14-15 0.10725220178148476 1102 16-17 0.2145057162656343 1102 18-19 0.36346203496191265 1102 20-21 0.2960176574293527 1102 22-23 0.42571788336763206 1102 24-25 0.2542264905777998 1102 26-27 0.18652753525395127 1102 28-29 0.1069944029897556 1102 30-31 0.06435329150696845 1102 32-33 0.3444843873597492 1102 34-35 0.21515565024163408 1102 36-37 0.06476661689917051 1102 38-39 0.2628436315876499 1102 40-41 0.36236554204385385 1102 42-43 0.36810122380865806 1102 44-45 0.4842426939283513 1102 46-47 0.4881014070984335 1102 48-49 0.4892986144683462 1102 50-51 0.18484585772424822 1102 52-53 0.11962690477572124 1102 54-55 -0.038371274753707496 1102 56-57 0.07668224009835001 1102 58-59 0.02380590128557003 1102 60-61 0.1432379079048829 1102 62-63 0.13791351395160945 1102 64-65 0.2327464233869847 1102 66-67 0.15733551021886427 1102 68-69 0.05205729480385202 1102 70-71 0.15841575916949324 1102 72-73 -0.008473237328686878 1102 74-75 0.10360158052178292 1102 76-77 -0.011633106392910264 1102 78-79 0.1972271567483439 1102 80-81 0.3108527055878163 1102 82-83 0.4254394396346157 1102 84-85 0.46789022481706866 1102 86-87 0.4070540926311459 1102 88-89 0.44401540730750355 1102 90-91 0.7360235586746633 1102 92-93 0.6734877281428666 1102 94-95 0.31378698139640093 1102 96-97 0.21284514060510418 1102 98-99 0.30025797782748853 1102 100-101 0.8025773930560618 1103 1 0.033005498004015976 1103 2 0.08917988575682045 1103 3 0.1616067525831184 1103 4 0.05752070837899481 1103 5 0.04510363281344354 1103 6 0.10233243121621882 1103 7 0.22907561013857247 1103 8 0.08071695176651161 1103 9 0.06250001063006749 1103 10-11 0.19052619240577684 1103 12-13 0.0605753601062915 1103 14-15 0.2871867915263593 1103 16-17 0.19595401459655193 1103 18-19 0.053618543407608854 1103 20-21 0.27203389721683635 1103 22-23 0.2506358072812205 1103 24-25 0.06735615826362817 1103 26-27 0.1659458601499395 1103 28-29 0.20474326269604148 1103 30-31 0.28516302535489046 1103 32-33 0.3928340941413708 1103 34-35 0.32345222685433583 1103 36-37 0.24488603447222346 1103 38-39 0.458162788522138 1103 40-41 0.26895543728719673 1103 42-43 0.30038218716889986 1103 44-45 0.4159409797285676 1103 46-47 0.1630080533818301 1103 48-49 0.3455777483463436 1103 50-51 0.4800221646762779 1103 52-53 0.38934082888980015 1103 54-55 0.41811586874786855 1103 56-57 0.3827383275125129 1103 58-59 0.4487470069945658 1103 60-61 0.271069846153587 1103 62-63 0.3645719521251394 1103 64-65 0.43489794461398645 1103 66-67 0.3337959633289884 1103 68-69 0.27153877442893304 1103 70-71 0.19882726736457101 1103 72-73 0.26790388662500675 1103 74-75 0.39694213989224636 1103 76-77 0.2354597711570925 1103 78-79 0.2905244852030542 1103 80-81 0.4654188927463565 1103 82-83 0.40281526039548154 1103 84-85 0.34374544510678007 1103 86-87 0.15485495474332467 1103 88-89 0.30034466398636894 1103 90-91 0.3157470061209082 1103 92-93 0.46931563150610955 1103 94-95 0.395183277321415 1103 96-97 0.6467613603434614 1103 98-99 0.698787500157195 1103 100-101 0.8138706866253074 1104 1 -0.09545431560399464 1104 2 0.10256075138175191 1104 3 0.060369978706027894 1104 4 0.018937217383268035 1104 5 0.12332169741998911 1104 6 0.07047586895708946 1104 7 0.18700894814338653 1104 8 0.18209678966621112 1104 9 0.024728188987104716 1104 10-11 -0.01328018649783047 1104 12-13 0.2541278441648771 1104 14-15 0.3234812581603279 1104 16-17 0.3011469466498937 1104 18-19 0.22232460231495565 1104 20-21 0.2486064335959739 1104 22-23 0.20617355282579553 1104 24-25 0.11939058705862493 1104 26-27 0.26300458063203536 1104 28-29 0.33650668735677414 1104 30-31 0.4083878636049505 1104 32-33 0.23709576699774715 1104 34-35 0.23215694271166853 1104 36-37 0.19567347053136075 1104 38-39 0.22859612652694494 1104 40-41 0.25005638793303575 1104 42-43 0.18124635225894536 1104 44-45 0.4996903665970365 1104 46-47 0.45886591197617577 1104 48-49 0.34019094994398813 1104 50-51 0.46692596725960556 1104 52-53 0.34616031129000646 1104 54-55 0.30551280425810745 1104 56-57 0.4177364589211976 1104 58-59 0.1964229465456384 1104 60-61 0.0050262379037562255 1104 62-63 0.15585760134383264 1104 64-65 0.18650668494986888 1104 66-67 0.21963817247107187 1104 68-69 0.06139524325849521 1104 70-71 0.15402195122227624 1104 72-73 0.19046829463191273 1104 74-75 0.18909493226838947 1104 76-77 -0.010306701643227001 1104 78-79 -0.08132226695166267 1104 80-81 0.10688293404222549 1104 82-83 -0.19765947858567046 1104 84-85 -0.006704476645960966 1104 86-87 -0.045326648975173356 1104 88-89 -0.09794265528581647 1104 90-91 0.06261273615617924 1104 92-93 0.14826390656275024 1104 94-95 0.037130524652834396 1104 96-97 -0.09573945716523724 1104 98-99 0.1574872525688491 1104 100-101 0.5383425176112233 1105 1 0.0836919697758276 1105 2 0.09618367318729071 1105 3 0.01576109037457485 1105 4 0.08185790804759563 1105 5 -0.003949509570269072 1105 6 0.07617844849637123 1105 7 0.011890144402983083 1105 8 0.05591815900996977 1105 9 0.0739303574166641 1105 10-11 0.12040990645477478 1105 12-13 0.18428378713907279 1105 14-15 0.14442137550080503 1105 16-17 0.13180737002322473 1105 18-19 0.13413250955744616 1105 20-21 0.1398407382631106 1105 22-23 0.08209367211730267 1105 24-25 0.18736444326777502 1105 26-27 0.20367517722888095 1105 28-29 0.15971278571651482 1105 30-31 -0.05487425716375327 1105 32-33 0.1920381419576742 1105 34-35 0.20938843232243443 1105 36-37 0.2827381471482795 1105 38-39 0.20473782698822873 1105 40-41 0.36351988278371294 1105 42-43 0.4427999289134803 1105 44-45 -0.12437000381978436 1105 46-47 -0.20869126014639505 1105 48-49 -0.04302741202766214 1105 50-51 -0.08342173400995989 1105 52-53 -0.08484990374127932 1105 54-55 -0.041201801982388986 1105 56-57 -0.015974537915347753 1105 58-59 -0.20179743613480383 1105 60-61 -0.10827978102122415 1105 62-63 -0.016152098058313413 1105 64-65 -0.23528491726315082 1105 66-67 -0.15482843009035463 1105 68-69 -0.13380801217303429 1105 70-71 -0.10923877595416087 1105 72-73 -0.02754527895672254 1105 74-75 0.20594764350214234 1105 76-77 0.04915369943262604 1105 78-79 -0.27636804645749535 1105 80-81 -0.19567709494449304 1105 82-83 -0.20217053674027596 1105 84-85 -0.09652707235090574 1105 86-87 -0.06121499436308042 1105 88-89 -0.11871972233153727 1105 90-91 -0.009260391204218621 1105 92-93 -0.10144274226593453 1105 94-95 -0.17607515562923126 1105 96-97 -0.28664348046610044 1105 98-99 -0.046370210814515644 1105 100-101 -0.15997681011986842 1106 1 -0.10775042996888118 1106 2 -0.04023266550008486 1106 3 -0.1756414199966656 1106 4 -0.023130922927833808 1106 5 0.006839236307826013 1106 6 0.03400474357135863 1106 7 0.09754592570284615 1106 8 0.014417254850840777 1106 9 -0.0808856196070522 1106 10-11 0.17168370711428338 1106 12-13 0.0627036360913209 1106 14-15 0.07776889172247792 1106 16-17 -0.011658750602236978 1106 18-19 0.026864665711052282 1106 20-21 0.006567682205243841 1106 22-23 0.007399490647237883 1106 24-25 0.10655655049041002 1106 26-27 -0.14794699062559857 1106 28-29 0.18412907121805944 1106 30-31 0.14278024767616415 1106 32-33 -0.10082564912837455 1106 34-35 0.11702059911320362 1106 36-37 0.07783308385714349 1106 38-39 0.1707786737066641 1106 40-41 -0.09227205424426899 1106 42-43 -0.049490784840372726 1106 44-45 -0.06208540643653038 1106 46-47 0.027734386061446514 1106 48-49 -0.06379780879396435 1106 50-51 0.05805523652508526 1106 52-53 0.01567796563431756 1106 54-55 -0.1417655717941173 1106 56-57 0.2810045068671272 1106 58-59 0.10362087042832968 1106 60-61 -0.014832275436710063 1106 62-63 -0.05250328410235028 1106 64-65 0.06516849194863283 1106 66-67 0.14179426962244435 1106 68-69 -0.02716643149012299 1106 70-71 -0.11722728781460745 1106 72-73 -0.20632280262441327 1106 74-75 0.014788458309070052 1106 76-77 -0.3353639050775321 1106 78-79 -0.18950760607880923 1106 80-81 -0.2604680746275072 1106 82-83 0.1766162944478964 1106 84-85 0.0053384206817490565 1106 86-87 -0.18878918859773464 1106 88-89 0.005104870796852623 1106 90-91 0.15422088738316475 1106 92-93 0.1719364195253661 1106 94-95 0.11186628677051402 1106 96-97 -0.08554386340408371 1106 98-99 -0.19801897158464854 1106 100-101 0.00748808723147576 1107 1 -0.07130492831226576 1107 2 0.09866802263176311 1107 3 0.07027014231749718 1107 4 0.14709675526433585 1107 5 0.1855942273875968 1107 6 0.11111161639440326 1107 7 -0.0938991511810201 1107 8 0.15639754539540007 1107 9 0.1211893952599894 1107 10-11 0.14838492196433606 1107 12-13 -0.07135464272950998 1107 14-15 0.07897949405023041 1107 16-17 -0.007326068587119039 1107 18-19 0.1155041186037522 1107 20-21 0.049044956863305345 1107 22-23 -0.05699605773437355 1107 24-25 0.029439058348707192 1107 26-27 -0.04794486676185983 1107 28-29 -0.062265670095342784 1107 30-31 0.012417491750575493 1107 32-33 0.02365931391639009 1107 34-35 -0.0610122782973761 1107 36-37 -0.14763098036850408 1107 38-39 0.04752024196764637 1107 40-41 -0.10405949796187741 1107 42-43 -0.19671701880520231 1107 44-45 -0.23137327492688797 1107 46-47 -0.16967343825254488 1107 48-49 -0.6243863314343514 1107 50-51 -0.5007120729643333 1107 52-53 -0.5009784356909748 1107 54-55 -0.330407148550556 1107 56-57 -0.2042027822332102 1107 58-59 -0.07645622582516864 1107 60-61 -0.01708357099359148 1107 62-63 -0.0622465089770472 1107 64-65 -0.007759678979915918 1107 66-67 0.11253804670170808 1107 68-69 0.13752327201849823 1107 70-71 0.1811861859769195 1107 72-73 0.07385199136079734 1107 74-75 0.041798695332261104 1107 76-77 -0.031247857163169357 1107 78-79 0.07663375829124774 1107 80-81 0.29518777449321476 1107 82-83 0.46293436428053525 1107 84-85 0.21915309233241587 1107 86-87 0.4773925291832519 1107 88-89 0.3622006995284792 1107 90-91 0.21690141580046074 1107 92-93 0.2923435642029588 1107 94-95 0.27265870032926287 1107 96-97 0.047797611406892315 1107 98-99 0.12223844069978185 1107 100-101 0.2279398330474649 1108 1 -0.12904575689378817 1108 2 -0.03216661429612344 1108 3 0.06556206339536175 1108 4 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-1.4271199808007466 2316 98-99 -1.8869192180280194 2316 100-101 -1.7404185443356788 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 6.0 5 24.0 6 50.0 7 119.0 8 239.0 9 413.0 10 819.0 11 1423.0 12 2489.0 13 3873.0 14 4572.0 15 6157.0 16 7916.0 17 11035.0 18 9402.0 19 4789.0 20 3555.0 21 3352.0 22 3389.0 23 3763.0 24 4213.0 25 4586.0 26 5173.0 27 6285.0 28 7671.0 29 9500.0 30 11868.0 31 14319.0 32 17645.0 33 22813.0 34 30430.0 35 46855.0 36 66587.0 37 131003.0 38 195144.0 39 41774.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.99050602727866 23.294276469768228 14.115351034872376 20.599866468080734 2 14.194915874498276 24.44045635925556 40.31349082041028 21.051136945835882 3 17.477706111445293 31.110457381461497 32.17315834844474 19.23867815864848 4 9.956400135234222 19.140765068590387 42.918132069976394 27.984702726198996 5 8.724070959914176 43.16825683964646 36.81663907829974 11.291033122139629 6 24.30879066356974 43.377986971265656 18.84892844441127 13.464293920753331 7 23.478212507994773 37.614433179804436 24.35094159023152 14.556412721969266 8 24.040371220869392 35.79989432996858 22.237540961772915 17.922193487389116 9 26.055126972348695 16.818512452818037 24.332646917201238 32.793713657632026 10-11 21.443379692136567 29.539082995795873 30.17229960885903 18.845237703208525 12-13 23.64741894905076 28.554858812587035 30.088319428152012 17.709402810210193 14-15 20.798408802518516 30.001434302365578 29.617904776958664 19.582252118157243 16-17 16.855762067638196 33.1102095417071 31.134587041696122 18.899441348958586 18-19 16.569851256990393 31.408733730547272 34.886477894912474 17.13493711754986 20-21 17.382500632995686 30.374206560030675 34.72175265894778 17.521540148025856 22-23 17.69329053843418 30.321883795164062 33.99523460356907 17.989591062832687 24-25 17.97232325154227 31.091994818842434 33.96139013091936 16.97429179869593 26-27 16.966193629215915 30.421928330142674 35.62848704123271 16.983390999408705 28-29 16.61378162674188 30.43624303505488 35.94358083434444 17.0063945038588 30-31 17.27766621978421 30.683700889276576 35.04431746998179 16.99431542095742 32-33 16.534687446716177 31.347534546210294 35.47456536868196 16.643212638391574 34-35 17.18908365233542 30.506234536235244 35.075861634934775 17.228820176494565 36-37 17.527540800779793 29.92554799863595 34.49731361021223 18.049597590372027 38-39 16.64252987705239 31.453615286161824 34.6734069170014 17.230447919784385 40-41 16.301026923349937 31.023432703333825 35.937560601503534 16.7379797718127 42-43 16.412301725241953 31.119778993395414 34.1614738652372 18.306445416125435 44-45 16.787208491374482 30.815115454503538 33.93007817960012 18.46759787452185 46-47 17.92347951078528 30.832442275166084 33.24148298426542 18.002595229783225 48-49 17.477327669428604 31.30512554590346 32.9029292702331 18.314617514434833 50-51 17.211157419435104 31.262948746564987 33.070778553106784 18.455115280893118 52-53 16.934871844786667 32.17496115787941 32.9282384961092 17.961928501224726 54-55 17.647351539797338 31.36257227347603 32.41166737772327 18.578408809003367 56-57 18.385673523959788 32.00324630418221 31.07853260611046 18.53254756574754 58-59 17.630589096231247 30.890084156604463 32.351628247347236 19.12769849981705 60-61 18.00285400658617 31.91650201244054 31.926454445664103 18.154189535309186 62-63 17.652530006922703 32.00294471057096 32.281682348860386 18.062842933645953 64-65 17.713669334301255 31.808176192678545 32.09606411689977 18.382090356120436 66-67 17.72966034841839 32.039973128784254 30.89472659708837 19.335639925708993 68-69 17.49036602518225 31.492011082181136 31.882126689878948 19.135496202757665 70-71 18.34065609184232 31.177973766864053 32.02575015587084 18.455619985422782 72-73 18.040847450309148 30.713158224045532 31.80024281027648 19.445751515368837 74-75 17.694461397508118 32.057901908353934 30.997030408673726 19.250606285464222 76-77 17.37779172428153 31.373768092778135 31.86528459421295 19.38315558872738 78-79 17.136433958615356 31.001564670100734 31.96944791172213 19.892553459561775 80-81 17.882998816684097 31.660542744634277 30.831489814482115 19.624968624199504 82-83 17.974443073563613 31.392341703512137 30.98978571815373 19.64342950477052 84-85 17.184897563717154 30.654193023385158 31.3466880545156 20.814221358382095 86-87 17.497212221386988 31.496989492162715 31.15835315183832 19.847445134611974 88-89 17.698996507832536 30.82969335899408 31.344870856041005 20.126439277132373 90-91 17.477616607236968 30.291677737864376 31.91257448285885 20.31813117203981 92-93 18.085640394884003 31.171677082813503 31.18620782781508 19.556474694487417 94-95 17.56226001418235 30.154842307317963 31.669895139873265 20.613002538626418 96-97 17.64879793689498 30.126734012253348 31.29030416748385 20.934163883367827 98-99 17.415511206506046 30.64977756766824 30.762182917753094 21.17252830807262 100-101 17.025550583422675 30.670798862792303 31.11485139095722 21.1887991628278 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2185.0 1 1322.5 2 782.0 3 1040.0 4 716.0 5 687.5 6 1555.0 7 2490.0 8 2881.0 9 2727.5 10 1982.0 11 1434.5 12 1415.5 13 1476.0 14 1500.0 15 1523.0 16 1588.5 17 1640.0 18 1769.5 19 1998.5 20 2292.0 21 2688.0 22 3106.5 23 3631.0 24 4111.0 25 5051.0 26 7517.5 27 10081.0 28 10806.5 29 12078.5 30 15260.0 31 18276.0 32 22319.5 33 27088.5 34 31408.5 35 35825.0 36 42813.5 37 48516.5 38 44563.0 39 37787.0 40 32568.5 41 26986.5 42 22061.5 43 22021.5 44 23282.0 45 21472.5 46 17275.0 47 15907.5 48 23510.0 49 21871.0 50 12676.0 51 7986.5 52 4401.5 53 2819.5 54 2165.0 55 1767.5 56 1412.5 57 1184.5 58 1027.0 59 916.5 60 837.0 61 730.0 62 509.0 63 276.0 64 172.0 65 135.0 66 90.0 67 50.5 68 44.0 69 45.0 70 38.5 71 39.0 72 33.0 73 32.5 74 34.0 75 19.5 76 9.5 77 4.0 78 1.5 79 1.0 80 1.0 81 1.0 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25963799964581513 2 0.016538384419378303 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.195360763634288E-4 12-13 7.317869212114293E-5 14-15 0.0 16-17 8.781443054537152E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0020490033793920024 26-27 0.002195360763634288 28-29 0.002341718147876574 30-31 0.0010245016896960012 32-33 0.001097680381817144 34-35 0.0012440377660594298 36-37 0.0 38-39 7.317869212114293E-5 40-41 6.586082290902863E-4 42-43 0.003146683761209146 44-45 0.0035857559139360036 46-47 0.012074484199988585 48-49 0.015001631884834301 50-51 0.005342044524843434 52-53 0.006512903598781721 54-55 5.122508448480006E-4 56-57 0.0030003263769668603 58-59 0.0013172164581805727 60-61 0.0013172164581805727 62-63 0.0 64-65 0.0010245016896960012 66-67 0.0 68-69 0.0 70-71 1.4635738424228587E-4 72-73 0.0013903951503017157 74-75 0.0 76-77 0.1885083109040642 78-79 0.10054752297445038 80-81 0.0013903951503017157 82-83 0.0 84-85 7.317869212114293E-4 86-87 0.11942762554170527 88-89 0.5680861869364326 90-91 0.30178892630759346 92-93 0.28466511235124603 94-95 0.0022685394557554308 96-97 0.0024880755321188596 98-99 0.0025612542242400027 100-101 9.513229975748582E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 683259.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.451828468783702 #Duplication Level Percentage of deduplicated Percentage of total 1 69.86312586469938 12.891024147484792 2 10.707169248273647 3.951337007107622 3 4.648557565235225 2.5732316046296155 4 2.692527052206981 1.9872818925953213 5 1.8236696713398712 1.6825019979643228 6 1.2777107458992067 1.4145659709632308 7 0.9400871707872673 1.214242905474958 8 0.7099513280696675 1.0479920101381353 9 0.608398915130648 1.0103465180326332 >10 5.069407167070022 19.54529789327266 >50 0.8193859059467519 10.76197905843481 >100 0.7583087377396853 27.208940988999704 >500 0.06266358816049701 7.705214028713457 >1k 0.017450619487733344 5.127930073321393 >5k 0.001586419953430304 1.8781139028673395 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7257 1.0621155374462685 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5575 0.8159424171507437 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3959 0.5794288842152098 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3808 0.5573289191946246 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2701 0.3953112948384141 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1964 0.28744590265184944 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1802 0.2637360064045991 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1692 0.24763669413794767 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1579 0.2310983097185694 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1540 0.22539037173312024 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1312 0.19202088812587906 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 1298 0.18997188474648705 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1283 0.18777652398285277 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1209 0.17694607754892364 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1183 0.17314078555862417 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1173 0.17167721171620132 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1148 0.16801827711014417 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1107 0.16201762435621045 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1092 0.15982226359257617 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1061 0.1552851846810653 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1055 0.1544070403756116 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1035 0.1514798926907659 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1031 0.15089446315379673 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1004 0.14694281377925503 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 983 0.143869308710167 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 971 0.14211302009925958 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 968 0.14167394794653274 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 919 0.13450243611866072 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 900 0.1317216458180573 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 877 0.12835542598048472 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 876 0.12820906859624243 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 871 0.127477281675031 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 867 0.12689185213806187 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 850 0.12440377660594298 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 846 0.12381834706897384 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 839 0.12279384537927783 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 821 0.12015941246291671 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 817 0.11957398292594755 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 795 0.11635412047261726 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 774 0.11328061540352927 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 771 0.1128415432508024 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 746 0.10918260864474527 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 736 0.1077190348023224 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 731 0.10698724788111096 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 718 0.10508460188596125 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 718 0.10508460188596125 No Hit CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG 718 0.10508460188596125 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 716 0.10479188711747668 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 715 0.1046455297332344 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 714 0.10449917234899211 No Hit TGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAA 711 0.10406010019626524 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 710 0.10391374281202297 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 706 0.10332831327505383 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 703 0.10288924112232696 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 686 0.1004011655902081 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.4635738424228587E-4 0.0 14-15 0.0 0.0 0.0 7.317869212114293E-4 0.0 16-17 0.0 0.0 0.0 0.002195360763634288 0.0 18-19 0.0 0.0 0.0 0.0045370789115108615 0.0 20-21 0.0 0.0 0.0 0.008561906978173724 0.0 22-23 0.0 0.0 0.0 0.016318848343014874 0.0 24-25 0.0 0.0 0.0 0.026856580008459457 0.0 26-27 0.0 0.0 0.0 0.03395491314421032 0.0 28-29 0.0 0.0 0.0 0.04398039396480691 0.0 30-31 0.0 0.0 0.0 0.07047108051266064 0.0 32-33 0.0 0.0 0.0 0.11518326139867897 0.0 34-35 0.0 0.0 0.0 0.18960599128588135 0.0 36-37 0.0 0.0 0.0 0.29783727693305173 0.0 38-39 0.0 0.0 0.0 0.4402430118007959 0.0 40-41 0.0 0.0 0.0 0.6062122855315482 0.0 42-43 0.0 0.0 0.0 0.755643174842922 0.0 44-45 0.0 0.0 0.0 0.9225637715712489 0.0 46-47 0.0 0.0 0.0 1.1282690751237818 0.0 48-49 0.0 0.0 0.0 1.3461220415684243 0.0 50-51 0.0 0.0 0.0 1.568146193463972 0.0 52-53 0.0 0.0 0.0 1.7718024936371126 0.0 54-55 0.0 0.0 0.0 1.9826303056381254 0.0 56-57 0.0 0.0 0.0 2.2465858481190883 0.0 58-59 0.0 0.0 0.0 2.5196155484230722 0.0 60-61 0.0 0.0 0.0 2.8293809521718702 0.0 62-63 0.0 0.0 0.0 3.104166941086762 0.0 64-65 0.0 0.0 0.0 3.3834900089131645 0.0 66-67 0.0 0.0 0.0 3.724868607658297 0.0 68-69 0.0 0.0 0.0 4.100202119547639 0.0 70-71 0.0 0.0 0.0 4.504514393516953 0.0 72-73 0.0 0.0 0.0 4.892580997835374 0.0 74-75 0.0 0.0 0.0 5.289794938668939 0.0 76-77 0.0 0.0 0.0 5.714597246432173 0.0 78-79 0.0 0.0 0.0 6.23497092610562 0.0 80-81 0.0 0.0 0.0 6.777078677339047 0.0 82-83 0.0 0.0 0.0 7.292256669871893 0.0 84-85 0.0 0.0 0.0 7.836193888408349 0.0 86-87 0.0 0.0 0.0 8.416866810389617 0.0 88-89 0.0 0.0 0.0 9.039251586879939 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACAGG 110 0.0 51.886967 1 GTCAACC 45 9.677638E-4 42.173077 8 ATGGGTA 305 0.0 42.000237 5 CATGGGT 365 0.0 38.995655 4 ATGGGAC 365 0.0 36.395943 5 TGGGAAC 355 0.0 34.74824 6 GGTCAAC 55 0.002593667 34.505245 7 TAGGCAT 220 0.0 34.505245 5 TAGATAT 160 5.4569682E-11 32.61824 7 GATGACC 335 0.0 32.573982 9 GTAAGTA 90 2.3897663E-5 31.708702 1 TACAGGG 105 1.913153E-6 31.639069 2 GGGATGA 510 0.0 31.62981 7 TGTAGAT 180 5.456968E-12 31.629808 5 GTAGATA 165 7.8216544E-11 31.629807 6 ACTGGTA 135 1.20926416E-8 31.629807 8 GGATGAC 425 0.0 31.257692 8 GCAGATC 140 1.7233106E-8 30.500172 8 CCTTTAG 235 0.0 30.359396 1 GTACATG 5625 0.0 30.271244 1 >>END_MODULE