FastQCFastQC Report
Thu 26 May 2016
SRR936363_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936363_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260796
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55510.24553298926572767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38680.17109018239593488No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27200.12031160706229133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33800.048.0437241
GTATCAA63650.041.2069631
ATCAACG78500.032.919813
TCAACGC78550.032.8988574
CAACGCA79700.032.3053865
TATCAAC81200.032.000762
AACGCAG81600.031.5510836
TACATGG94800.027.5597132
GTACATG96000.027.222441
ACATGGG94450.026.3082333
ACGCAGA99350.025.5806717
CGCAGAG100650.025.1562278
GAGTACA72350.022.8373721
AGTACAT67500.022.5785482
CATGGGG59150.022.484644
TACCTGG20900.021.7404692
GTACCTG22350.021.6063751
GCAGAGT114000.021.3800099
GTATACG1150.003796319720.5840781
TATAGGG8150.020.3261152
Produced by FastQC (version 0.11.5)