##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936363_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2260796 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.585320391578897 33.0 31.0 34.0 30.0 34.0 2 31.675450593507772 33.0 31.0 34.0 30.0 34.0 3 31.742541122684223 34.0 31.0 34.0 30.0 34.0 4 35.23116636795182 37.0 35.0 37.0 33.0 37.0 5 35.206012395634104 37.0 35.0 37.0 33.0 37.0 6 35.29708783985817 37.0 35.0 37.0 33.0 37.0 7 35.23603412249491 37.0 35.0 37.0 33.0 37.0 8 35.23543079517126 37.0 35.0 37.0 33.0 37.0 9 36.88089416294084 39.0 37.0 39.0 33.0 39.0 10-11 36.79239214860607 39.0 37.0 39.0 33.0 39.0 12-13 36.689491223445195 39.0 37.0 39.0 33.0 39.0 14-15 37.90368281791015 40.0 38.0 41.0 33.0 41.0 16-17 37.84282173181481 40.0 38.0 41.0 33.0 41.0 18-19 37.790022850358895 40.0 38.0 41.0 32.0 41.0 20-21 37.70430038800494 40.0 38.0 41.0 32.0 41.0 22-23 37.536519217125296 40.0 38.0 41.0 32.0 41.0 24-25 37.69459274521009 40.0 38.0 41.0 32.0 41.0 26-27 37.642804127395834 40.0 38.0 41.0 32.0 41.0 28-29 37.5071746853763 40.0 38.0 41.0 32.0 41.0 30-31 37.34191010599807 40.0 37.0 41.0 31.5 41.0 32-33 37.14653002747705 40.0 37.0 41.0 31.0 41.0 34-35 36.87312654480988 40.0 36.5 41.0 30.5 41.0 36-37 36.66335109403944 40.0 36.0 41.0 30.0 41.0 38-39 36.486067738973354 40.0 36.0 41.0 30.0 41.0 40-41 36.289272672103095 40.0 36.0 41.0 30.0 41.0 42-43 36.08622206514873 39.5 35.0 41.0 29.0 41.0 44-45 35.87623828067636 39.0 35.0 41.0 28.0 41.0 46-47 35.58202774597973 39.0 35.0 41.0 27.0 41.0 48-49 35.38831876029505 39.0 35.0 41.0 26.5 41.0 50-51 35.070434262976406 38.5 34.5 40.5 26.0 41.0 52-53 35.0782474402821 39.0 35.0 40.0 26.0 41.0 54-55 35.14333557738071 39.0 35.0 41.0 26.0 41.0 56-57 34.9664918904669 39.0 35.0 41.0 26.0 41.0 58-59 34.715224195371896 38.0 34.5 40.5 26.0 41.0 60-61 34.404544240170274 38.0 34.0 40.0 25.0 41.0 62-63 34.07866278071971 37.0 34.0 40.0 23.5 41.0 64-65 33.72075388491487 37.0 34.0 40.0 23.0 41.0 66-67 33.35538854456571 36.0 33.0 39.0 22.0 41.0 68-69 32.969139188144354 35.5 33.0 39.0 21.5 41.0 70-71 32.562906825737485 35.0 33.0 38.5 20.0 40.0 72-73 32.158485329945734 35.0 32.5 37.5 19.0 40.0 74-75 31.748381101169677 35.0 32.0 37.0 18.0 39.0 76-77 31.35879022255878 35.0 32.0 36.5 15.0 39.0 78-79 30.972119775512695 35.0 31.0 36.0 10.0 38.5 80-81 30.59400317410328 35.0 31.0 35.5 7.0 37.0 82-83 30.251012254091037 35.0 31.0 35.0 4.5 37.0 84-85 29.941017455798754 34.0 31.0 35.0 2.0 36.5 86-87 29.61666045941341 34.0 30.0 35.0 2.0 36.0 88-89 29.28885578353819 34.0 30.0 35.0 2.0 36.0 90-91 28.9361791156743 34.0 29.5 35.0 2.0 35.0 92-93 28.6169515073452 34.0 29.0 35.0 2.0 35.0 94-95 28.220041082875234 34.0 29.0 35.0 2.0 35.0 96-97 27.77397076074091 34.0 29.0 35.0 2.0 35.0 98-99 27.25210832821714 34.0 27.5 35.0 2.0 35.0 100-101 23.942693635339058 29.5 13.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.42431722868896316 1101 2 0.32233248273916715 1101 3 0.4517920219863143 1101 4 0.18624914145370042 1101 5 0.430876576028119 1101 6 0.381453229114463 1101 7 0.46393927541140556 1101 8 0.3929220793744932 1101 9 0.4103687304547279 1101 10-11 0.5778940222005389 1101 12-13 0.519771268433189 1101 14-15 0.682800126732829 1101 16-17 0.7129092100357752 1101 18-19 0.6726872213695927 1101 20-21 0.6586660399126814 1101 22-23 0.6930842295403963 1101 24-25 0.6288861781943993 1101 26-27 0.6117426520670932 1101 28-29 0.6044895074488608 1101 30-31 0.6789478086403946 1101 32-33 0.673107720151485 1101 34-35 0.5749188715535283 1101 36-37 0.6714646930155794 1101 38-39 0.6451425553756991 1101 40-41 0.5906828584285293 1101 42-43 0.5671951417516183 1101 44-45 0.447434007426871 1101 46-47 0.3756343661071071 1101 48-49 0.35400307298720435 1101 50-51 0.5331635828927972 1101 52-53 0.5191909563065025 1101 54-55 0.5625517012360106 1101 56-57 0.5546379481000159 1101 58-59 0.5654840553567553 1101 60-61 0.5553683075176963 1101 62-63 0.539443696009144 1101 64-65 0.5260057326864569 1101 66-67 0.537267888078361 1101 68-69 0.4537969128665793 1101 70-71 0.6981670638528215 1101 72-73 0.7681698414909945 1101 74-75 0.6131763266314287 1101 76-77 0.48452059121045465 1101 78-79 0.632295591496316 1101 80-81 0.6117156532097603 1101 82-83 0.6551945062952313 1101 84-85 0.6195218678369372 1101 86-87 0.5962243446006461 1101 88-89 0.5264785553333304 1101 90-91 0.6915711416914618 1101 92-93 0.747720278940367 1101 94-95 1.0449559817084229 1101 96-97 1.210599156803994 1101 98-99 1.235607157351648 1101 100-101 1.5281623701273581 1102 1 0.3932803128262492 1102 2 0.2807366134007161 1102 3 0.3327794720063011 1102 4 0.2946136150643568 1102 5 0.43274894782562967 1102 6 0.33421097621427975 1102 7 0.20707839466627576 1102 8 0.26965588880337776 1102 9 0.29184899316159374 1102 10-11 0.4457503476583611 1102 12-13 0.47974256453319697 1102 14-15 0.5362557219102797 1102 16-17 0.4379300141668594 1102 18-19 0.4980526252334201 1102 20-21 0.5279934204695493 1102 22-23 0.44548000057999815 1102 24-25 0.47849999656721565 1102 26-27 0.5656837824407006 1102 28-29 0.4394273165444602 1102 30-31 0.5075847873637542 1102 32-33 0.33599299209232925 1102 34-35 0.2710008393026442 1102 36-37 0.39260957414462183 1102 38-39 0.3729091620294298 1102 40-41 0.4508105414758745 1102 42-43 0.5258003262781941 1102 44-45 0.3809761467349233 1102 46-47 0.6962323543555442 1102 48-49 0.5573958866233326 1102 50-51 0.5980682530378871 1102 52-53 0.5257406979555768 1102 54-55 0.401025157906993 1102 56-57 0.4321700402520321 1102 58-59 0.5372112675820873 1102 60-61 0.41566052513788065 1102 62-63 0.492719041452645 1102 64-65 0.46499096043499577 1102 66-67 0.4591379606106898 1102 68-69 0.45000879437215247 1102 70-71 0.5728680539079747 1102 72-73 0.5120736246259909 1102 74-75 0.5423118925723465 1102 76-77 0.5353582145718363 1102 78-79 0.36597225891182816 1102 80-81 0.36947119069464307 1102 82-83 0.41679603941994614 1102 84-85 0.47665641914799295 1102 86-87 0.5076135121084455 1102 88-89 0.4374492671246557 1102 90-91 0.513751324978756 1102 92-93 0.5141260106498713 1102 94-95 0.6796022165562086 1102 96-97 0.5708693573779655 1102 98-99 0.645333708274574 1102 100-101 0.8950060432984159 1103 1 0.34777038513711744 1103 2 0.2988854581180824 1103 3 0.3433145907168793 1103 4 0.1377319615170407 1103 5 0.20739853157598276 1103 6 0.19903678427566263 1103 7 0.2784388822530772 1103 8 0.3445942646855187 1103 9 0.3324873127763084 1103 10-11 0.3928372847653776 1103 12-13 0.45789842809875125 1103 14-15 0.43549632716421627 1103 16-17 0.48461587785666893 1103 18-19 0.4430962863741783 1103 20-21 0.5227486825137291 1103 22-23 0.5847150069839344 1103 24-25 0.6106854726735946 1103 26-27 0.6583499482199215 1103 28-29 0.6124807209013738 1103 30-31 0.47441350287142825 1103 32-33 0.5906364495647765 1103 34-35 0.5491796256961194 1103 36-37 0.480064219782804 1103 38-39 0.5887053993892337 1103 40-41 0.6657508495698394 1103 42-43 0.460291245516558 1103 44-45 0.4393787704858596 1103 46-47 0.595454956061019 1103 48-49 0.642246737367067 1103 50-51 0.6535638108524822 1103 52-53 0.6702611404777272 1103 54-55 0.5717458348809856 1103 56-57 0.6636771629375957 1103 58-59 0.7426631984880245 1103 60-61 0.6683201690682594 1103 62-63 0.5165791949595189 1103 64-65 0.47150190570497585 1103 66-67 0.5236209658948567 1103 68-69 0.47093627126105275 1103 70-71 0.5308141735981025 1103 72-73 0.5778938371337929 1103 74-75 0.4765294621592382 1103 76-77 0.46708546233387693 1103 78-79 0.5996354967054884 1103 80-81 0.4095529217332903 1103 82-83 0.4298751102441294 1103 84-85 0.5519428213721156 1103 86-87 0.454272547975318 1103 88-89 0.37021615641829797 1103 90-91 0.6332024601785591 1103 92-93 0.6634220845830896 1103 94-95 0.4631934781916378 1103 96-97 0.402039306564852 1103 98-99 0.6734205117496117 1103 100-101 0.8824999163865854 1104 1 0.4047274215816188 1104 2 0.3662828054412408 1104 3 0.4322280189019736 1104 4 0.4240225882033002 1104 5 0.3275436069868789 1104 6 0.42172116594621656 1104 7 0.49698815733826507 1104 8 0.3982392259959866 1104 9 0.5617958447149363 1104 10-11 0.4704794638743408 1104 12-13 0.6424306917588112 1104 14-15 0.656895923409266 1104 16-17 0.6722177836500975 1104 18-19 0.584315273074786 1104 20-21 0.6940114798864485 1104 22-23 0.6490428338159759 1104 24-25 0.7626921805137314 1104 26-27 0.6770920992538549 1104 28-29 0.7119541559373559 1104 30-31 0.5821979012313037 1104 32-33 0.666797877586383 1104 34-35 0.6565354784155772 1104 36-37 0.689592019172359 1104 38-39 0.6170321080857306 1104 40-41 0.7226097207200439 1104 42-43 0.7450888352483886 1104 44-45 0.820284684099434 1104 46-47 0.9362571234339327 1104 48-49 0.831601386314837 1104 50-51 0.6927037855606315 1104 52-53 0.7687371090784652 1104 54-55 0.9713709091435803 1104 56-57 0.8343558342344508 1104 58-59 0.8120794422045918 1104 60-61 0.826257423077223 1104 62-63 0.8849701891513462 1104 64-65 0.8872218307538873 1104 66-67 0.975754133016224 1104 68-69 0.8947641387215981 1104 70-71 0.9063835201408068 1104 72-73 1.009707639831042 1104 74-75 1.0012240549666735 1104 76-77 1.1174242116666733 1104 78-79 0.9830686496676933 1104 80-81 0.9796491456173122 1104 82-83 1.0934014606312168 1104 84-85 0.8852296873666354 1104 86-87 0.8419515019687616 1104 88-89 0.9170178897716816 1104 90-91 1.0918742630675133 1104 92-93 1.022643929389961 1104 94-95 0.8751108665617657 1104 96-97 1.0306806064436493 1104 98-99 1.0859749405735712 1104 100-101 1.3216778191477196 1105 1 0.38824207161258784 1105 2 0.2969851378140298 1105 3 0.4098865213683922 1105 4 0.3099646290742655 1105 5 0.34906359424239497 1105 6 0.4021147367681337 1105 7 0.4526793901315642 1105 8 0.5134316516995057 1105 9 0.45096501173274106 1105 10-11 0.5051497427353624 1105 12-13 0.47720367118759555 1105 14-15 0.6024401138752182 1105 16-17 0.5426468071416224 1105 18-19 0.5767421077575037 1105 20-21 0.6281037793705408 1105 22-23 0.6768200617207398 1105 24-25 0.7753610190216804 1105 26-27 0.6723979904676014 1105 28-29 0.6890786531570399 1105 30-31 0.717757701806498 1105 32-33 0.7027935075099023 1105 34-35 0.40519952265930215 1105 36-37 0.4556708558798661 1105 38-39 0.5005961052917485 1105 40-41 0.6004528391221626 1105 42-43 0.624795551837245 1105 44-45 0.6061367971671459 1105 46-47 0.717764375630523 1105 48-49 0.8332769279318981 1105 50-51 0.6288788469028859 1105 52-53 0.668936100265725 1105 54-55 0.6656580899069482 1105 56-57 0.7151126511672672 1105 58-59 0.7541264403221462 1105 60-61 0.6663664487556673 1105 62-63 0.6187230913025417 1105 64-65 0.5907921247914061 1105 66-67 0.5318722872150659 1105 68-69 0.6878479459669222 1105 70-71 0.7132136027820053 1105 72-73 0.7421985834225566 1105 74-75 0.7244592218279955 1105 76-77 0.7128900216626093 1105 78-79 0.7152727952074542 1105 80-81 0.8117082660781456 1105 82-83 0.7105166351467709 1105 84-85 0.698484564818795 1105 86-87 0.7398471536672524 1105 88-89 0.657944042454762 1105 90-91 0.649762222828123 1105 92-93 0.7130052525163997 1105 94-95 0.7265296803254309 1105 96-97 0.8032030907510581 1105 98-99 0.7110891177765915 1105 100-101 0.7179500100586012 1106 1 0.23726392066151192 1106 2 0.2601288846919445 1106 3 0.30934881247936374 1106 4 0.3208135692471572 1106 5 0.32277312223994414 1106 6 0.3096916217838981 1106 7 0.28727208557560857 1106 8 0.2480073801628322 1106 9 0.3114190409168458 1106 10-11 0.41266243751156395 1106 12-13 0.41812665237635827 1106 14-15 0.4655953224901168 1106 16-17 0.4511727826414784 1106 18-19 0.4306901346465608 1106 20-21 0.5253751414084533 1106 22-23 0.4089784466807771 1106 24-25 0.41712330169428213 1106 26-27 0.4318812240672756 1106 28-29 0.5320560153240166 1106 30-31 0.5893882782912598 1106 32-33 0.6043872755739912 1106 34-35 0.45619387346696527 1106 36-37 0.45374943556308267 1106 38-39 0.5268534104568801 1106 40-41 0.47025901392989056 1106 42-43 0.5736045690655018 1106 44-45 0.6209567328279917 1106 46-47 0.5717857305612597 1106 48-49 0.5702742867686439 1106 50-51 0.5598834771738765 1106 52-53 0.4111289200741268 1106 54-55 0.42912371304412744 1106 56-57 0.5936521957392173 1106 58-59 0.6374738225561813 1106 60-61 0.7082204027616115 1106 62-63 0.5975866781312931 1106 64-65 0.5627204582503396 1106 66-67 0.5660744036654108 1106 68-69 0.6643740604516211 1106 70-71 0.7226858935816125 1106 72-73 0.6919632342524764 1106 74-75 0.6593156049099314 1106 76-77 0.6729868191901218 1106 78-79 0.7744453660976198 1106 80-81 0.7396489123305905 1106 82-83 0.7617635728549388 1106 84-85 0.8189543019975574 1106 86-87 0.8129461862334786 1106 88-89 0.6154679282182478 1106 90-91 0.6526809045117687 1106 92-93 0.6823914719888045 1106 94-95 0.6337624876071537 1106 96-97 0.726896238392218 1106 98-99 0.7405524377363939 1106 100-101 0.6035906421869335 1107 1 0.05135350046903753 1107 2 0.05350261196027262 1107 3 0.14305248419218586 1107 4 0.0972904695291561 1107 5 0.01327235292959017 1107 6 0.039497106335517174 1107 7 0.08769252483710943 1107 8 0.13173544580052265 1107 9 -0.02205896326144341 1107 10-11 0.09803206242412443 1107 12-13 0.15132996387538356 1107 14-15 0.17637686777406714 1107 16-17 0.026789559776872807 1107 18-19 0.11503642491908295 1107 20-21 0.23020117570612797 1107 22-23 0.15793268766427815 1107 24-25 0.21691989242972198 1107 26-27 0.21395520125469147 1107 28-29 0.321647735057752 1107 30-31 0.22516052161163458 1107 32-33 0.27784421744905075 1107 34-35 0.2450974377326247 1107 36-37 0.29335332852975426 1107 38-39 0.3186970596361789 1107 40-41 0.35295508366044714 1107 42-43 -0.04538617263320788 1107 44-45 0.18388510960687654 1107 46-47 0.23096142946619125 1107 48-49 0.20592847583200324 1107 50-51 0.18090641315833267 1107 52-53 0.2534346537854901 1107 54-55 0.3668102862292315 1107 56-57 0.32761805468538086 1107 58-59 0.2806826535366511 1107 60-61 0.3302676225660548 1107 62-63 0.34430334601607626 1107 64-65 0.24200750071000954 1107 66-67 0.2340345257679246 1107 68-69 0.369185857049942 1107 70-71 0.29049222480175274 1107 72-73 0.13748255908178209 1107 74-75 0.23589805400303376 1107 76-77 0.2454358268699366 1107 78-79 0.21449136335828456 1107 80-81 0.20751596733841282 1107 82-83 0.2064598400149258 1107 84-85 0.33165634681593303 1107 86-87 -0.02046940936270758 1107 88-89 0.25522815112366715 1107 90-91 0.2767407417653729 1107 92-93 -0.030020052574545986 1107 94-95 0.09565647179951142 1107 96-97 0.5188242459400989 1107 98-99 0.6193706327568016 1107 100-101 0.47704454668614105 1108 1 -0.03194180620668163 1108 2 0.03308020999253003 1108 3 0.004743965571645248 1108 4 -0.026585309437649585 1108 5 -0.04301985318231516 1108 6 9.685164965844706E-5 1108 7 0.02498818951990245 1108 8 -0.006029801729830808 1108 9 0.06165370916858137 1108 10-11 0.016527022964510252 1108 12-13 -0.006542110738479323 1108 14-15 -0.014273490093877683 1108 16-17 0.14626966580632939 1108 18-19 0.03608137428334146 1108 20-21 0.15037452625347925 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-1.99660560559472 2316 84-85 -2.0895254435848543 2316 86-87 -2.2033914092757954 2316 88-89 -2.0872600142208704 2316 90-91 -2.259757623040599 2316 92-93 -2.190403289347561 2316 94-95 -2.258187811189284 2316 96-97 -2.442694550030023 2316 98-99 -2.279278176908857 2316 100-101 -2.1487967056215673 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23623.0 3 5179.0 4 4078.0 5 3365.0 6 3253.0 7 3606.0 8 4295.0 9 5162.0 10 6017.0 11 7226.0 12 8918.0 13 12258.0 14 12626.0 15 11399.0 16 13350.0 17 16353.0 18 15176.0 19 11378.0 20 11263.0 21 11789.0 22 12601.0 23 13860.0 24 15431.0 25 17658.0 26 20847.0 27 24733.0 28 29713.0 29 35804.0 30 43440.0 31 54263.0 32 68378.0 33 89300.0 34 124182.0 35 187432.0 36 311877.0 37 496157.0 38 443282.0 39 81450.0 40 74.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.85330235645536 17.44768226573736 12.782475937603719 23.916539440203564 2 15.894577912181882 18.669965622847556 40.26639085906606 25.1690656059045 3 20.53366516909999 22.95068115985625 27.641518281343174 28.87413538970059 4 13.02871353524344 14.61916701022172 35.273652659646935 37.0784667948879 5 14.581210925767085 36.97574229154105 33.071293872735616 15.371752909956246 6 35.10735331977391 33.897311677178315 16.35787860745462 14.637456395593157 7 30.989915897247165 31.19750766028203 19.081146571816443 18.731429870654363 8 27.758503159115648 35.05421788276657 18.31225413064933 18.875024827468447 9 27.21446917770344 15.953512404920383 18.940098404140016 37.89192001323617 10-11 26.655963707546913 25.634619199728586 25.764642680920964 21.94477441180354 12-13 26.850608942181513 22.485419250327514 26.84337635066435 23.820595456826627 14-15 23.5003096523047 24.067924444837654 24.74643899849597 27.685326904361673 16-17 23.682289562944973 27.28960497332527 25.33929044642513 23.68881501730462 18-19 23.578628347739574 25.969808494576398 27.29418436729511 23.15737879038892 20-21 24.269906753220155 25.191581537133168 27.4990086129583 23.039503096688378 22-23 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26.71626101362837 23.445554881029363 48-49 23.90885015420095 26.03425121783516 26.563889745375587 23.49300888258831 50-51 23.73638999823695 26.230983570729986 26.51740695130847 23.515219479724593 52-53 23.992861482482823 26.059745959297203 26.467578677191266 23.47981388102871 54-55 23.782354501017906 26.063931820520747 26.5704723480973 23.583241330364046 56-57 23.576826845904893 26.398579832943227 26.478305312357403 23.546288008794473 58-59 23.706641625595235 26.05461991098566 26.57528157314234 23.663456890276766 60-61 23.592138413423157 26.054062631682818 26.711987781612322 23.641811173281702 62-63 23.6918744846684 26.139218985093997 26.73274764253155 23.43615888770605 64-65 23.579784596227398 26.151577518217607 26.622635734318006 23.646002151236985 66-67 23.554397335649774 26.420436048016793 26.488102579350397 23.53706403698304 68-69 23.590047488176857 26.01860405565568 26.72285789734338 23.66849055882408 70-71 23.557577447948557 26.059647060261554 26.46668007516165 23.916095416628238 72-73 23.605593495414546 26.33159570112726 26.355999898649234 23.70681090480896 74-75 23.503211742014102 26.536122289158364 26.253526336462347 23.707139632365184 76-77 23.756801701264347 26.3317243215635 26.277759675963196 23.633714301208958 78-79 23.72268825009657 26.409311410291576 26.25359670736527 23.61440363224659 80-81 23.59527211268407 26.409284362266877 26.240605882253575 23.75483764279548 82-83 23.794320920974986 26.502190971840207 26.219070512286223 23.484417594898584 84-85 23.767903854570058 26.540524429213747 25.95774133543568 23.733830380780514 86-87 23.65556360662005 26.41291736496068 26.26633118918174 23.665187839237532 88-89 23.718109600826068 26.476169939856593 26.063891392857265 23.741829066460078 90-91 23.50039334309613 26.627126309605707 26.157115774503453 23.715364572794712 92-93 23.576433948727367 26.504548701531856 26.177127564848927 23.74188978489185 94-95 23.602807376598275 26.383490025998263 26.3460162559703 23.667686341433164 96-97 23.767547226829883 26.224820041974468 26.16893556175013 23.83869716944552 98-99 23.409322536072153 26.345508358456804 26.099039475680947 24.146129629790096 100-101 23.54725730071034 26.445943279506817 25.991293187425786 24.015506232357062 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 105.0 1 90.0 2 170.0 3 303.5 4 258.5 5 216.0 6 435.0 7 930.0 8 1520.5 9 1740.0 10 1619.0 11 1588.0 12 1731.0 13 1938.0 14 2165.5 15 2413.5 16 2727.0 17 2881.0 18 2936.5 19 3087.0 20 3272.5 21 3621.5 22 4177.5 23 4824.0 24 5694.5 25 6967.5 26 8855.0 27 11462.0 28 13945.0 29 16381.0 30 18826.0 31 21934.5 32 26727.0 33 30110.5 34 31957.5 35 34621.5 36 38749.5 37 43465.0 38 47608.5 39 51194.0 40 54916.5 41 58395.0 42 60670.5 43 64111.0 44 69870.5 45 74430.0 46 76980.0 47 80063.5 48 83246.5 49 85333.5 50 84920.5 51 85171.0 52 88391.5 53 90638.5 54 91340.0 55 87990.5 56 82544.5 57 76921.0 58 72451.5 59 67596.0 60 58659.0 61 49177.0 62 40315.5 63 31410.0 64 23061.5 65 16972.5 66 12378.0 67 9446.5 68 7298.5 69 5784.0 70 4622.0 71 3170.5 72 2355.0 73 1974.0 74 1478.0 75 1068.0 76 808.0 77 511.0 78 317.0 79 194.0 80 117.5 81 74.5 82 53.0 83 41.5 84 30.5 85 23.0 86 20.0 87 22.0 88 24.0 89 27.0 90 28.0 91 20.5 92 15.5 93 14.0 94 14.0 95 19.0 96 18.0 97 13.5 98 15.0 99 13.0 100 9.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046266890068807624 2 0.012517714999495753 3 0.01061573003490806 4 0.00530786501745403 5 0.008625280653362799 6 0.0057501871022418655 7 0.00482131072418741 8 0.008625280653362799 9 0.014242771130168311 10-11 0.002233726528178571 12-13 0.00864739675760219 14-15 0.008669512861841581 16-17 0.018444830935652752 18-19 0.011478258100244339 20-21 0.0032952995316693767 22-23 0.009620505344135428 24-25 0.02083337019350707 26-27 0.007254082190520508 28-29 0.017891928329667958 30-31 0.014021610087774396 32-33 0.012407134478298795 34-35 0.2699712844502556 36-37 0.4783713346980444 38-39 0.474456784247672 40-41 0.49562189600477 42-43 0.4846080760935529 44-45 0.500421090624718 46-47 0.5078078694406749 48-49 0.5140003786277045 50-51 0.5242622509947823 52-53 0.5126291801648624 54-55 0.5007749482925483 56-57 0.49546708327509426 58-59 0.4935650983105066 60-61 0.4897390122770918 62-63 0.48792549172946165 64-65 0.503915435094542 66-67 0.47733187779879294 68-69 0.5039817834072601 70-71 0.48560330078432556 72-73 0.49464878741823676 74-75 0.5074097795643658 76-77 0.4942949297504065 78-79 0.4940295364995338 80-81 0.49761234538631527 82-83 0.4964401918616275 84-85 0.49741330044816073 86-87 0.4983200607219758 88-89 0.5125849479563835 90-91 0.5362270633882934 92-93 0.5244834120371763 94-95 0.4963959596531487 96-97 0.46967970573196344 98-99 0.4604794063683765 100-101 0.46903833870902106 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2260796.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.78291482569352 #Duplication Level Percentage of deduplicated Percentage of total 1 72.06112894652927 35.87413044588384 2 14.331657380488668 14.269433573677784 3 5.409051274895567 8.078350167178048 4 2.6199924758741644 5.217234490816056 5 1.5478675044693844 3.852867806822907 6 0.9202320171996061 2.7487099279274476 7 0.6151508305480649 2.143680098149026 8 0.4270355003608836 1.7007257553610629 9 0.3020849004466614 1.3534800182157825 >10 1.5937276711818789 14.217194587963464 >50 0.11278236787078551 3.8813874226426743 >100 0.056087000824178064 4.966314898810021 >500 0.002312648946787604 0.7755804050104051 >1k 8.005323277340986E-4 0.6751069857144055 >5k 8.894803641489984E-5 0.2458034158271458 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5551 0.24553298926572767 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3868 0.17109018239593488 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2720 0.12031160706229133 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.423220847878358E-5 7 0.0 0.0 0.0 0.0 4.423220847878358E-5 8 0.0 0.0 0.0 0.0 4.423220847878358E-5 9 0.0 0.0 0.0 0.0 4.423220847878358E-5 10-11 0.0 0.0 0.0 0.0 4.423220847878358E-5 12-13 0.0 0.0 0.0 2.211610423939179E-5 1.9904493815452612E-4 14-15 0.0 0.0 0.0 8.846441695756716E-5 2.211610423939179E-4 16-17 4.423220847878358E-5 0.0 0.0 2.4327714663330968E-4 2.4327714663330968E-4 18-19 4.423220847878358E-5 0.0 0.0 3.5385766783026864E-4 2.6539325087270146E-4 20-21 4.423220847878358E-5 0.0 0.0 4.6443818902722753E-4 3.096254593514851E-4 22-23 4.423220847878358E-5 0.0 0.0 9.067602738150634E-4 3.096254593514851E-4 24-25 1.7692883391513432E-4 0.0 0.0 0.0013933145670816827 3.096254593514851E-4 26-27 1.7692883391513432E-4 0.0 0.0 0.0017471722349119514 3.9808987630905225E-4 28-29 1.9904493815452612E-4 0.0 0.0 0.0021231460069816117 4.8655429326661937E-4 30-31 2.6539325087270146E-4 0.0 0.0 0.00358280888678147 4.8655429326661937E-4 32-33 2.6539325087270146E-4 0.0 0.0 0.006280973603987269 4.8655429326661937E-4 34-35 2.6539325087270146E-4 0.0 0.0 0.008957022216953674 4.8655429326661937E-4 36-37 2.6539325087270146E-4 0.0 0.0 0.01568031790572878 5.086703975060111E-4 38-39 2.6539325087270146E-4 0.0 0.0 0.026229699627918664 5.307865017454029E-4 40-41 2.6539325087270146E-4 0.0 0.0 0.038017583187514484 5.529026059847948E-4 42-43 2.6539325087270146E-4 0.0 0.0 0.04976123453863153 5.971348144635783E-4 44-45 3.096254593514851E-4 0.0 0.0 0.06502134646381186 7.29831439899929E-4 46-47 3.3174156359087686E-4 0.0 0.0 0.08284692648076164 8.846441695756716E-4 48-49 3.5385766783026864E-4 0.0 0.0 0.1031716262767627 8.846441695756716E-4 50-51 3.5385766783026864E-4 0.0 0.0 0.12641565183236347 9.288763780544552E-4 52-53 3.5385766783026864E-4 0.0 0.0 0.14990295453459757 0.0011058052119695895 54-55 3.5385766783026864E-4 0.0 0.0 0.1753585905141375 0.0011942696289271566 56-57 3.980898763090522E-4 0.0 0.0 0.20537014396699216 0.0011942696289271566 58-59 3.980898763090522E-4 0.0 0.0 0.23938471228717673 0.0011942696289271566 60-61 3.980898763090522E-4 0.0 0.0 0.279702370315588 0.0012163857331665485 62-63 3.980898763090522E-4 0.0 0.0 0.32364706943925947 0.0012827340458847238 64-65 3.980898763090522E-4 0.0 0.0 0.36801197454347934 0.0012827340458847238 66-67 4.423220847878358E-4 0.0 0.0 0.42122332134345597 0.0012827340458847238 68-69 4.423220847878358E-4 0.0 0.0 0.4805829451219836 0.0012827340458847238 70-71 4.423220847878358E-4 0.0 0.0 0.5487226622835497 0.0012827340458847238 72-73 5.086703975060111E-4 0.0 0.0 0.6181451134910005 0.0013933145670816827 74-75 5.750187102241865E-4 0.0 0.0 0.6891156919952088 0.0015038950882786417 76-77 5.750187102241865E-4 0.0 0.0 0.7679596036086405 0.0015038950882786417 78-79 5.750187102241865E-4 0.0 0.0 0.8626386458574767 0.0015038950882786417 80-81 6.192509187029702E-4 0.0 0.0 0.9713835304025662 0.0015260111925180335 82-83 6.192509187029702E-4 0.0 0.0 1.0880902124738365 0.0015923595052362088 84-85 6.634831271817537E-4 0.0 0.0 1.204067063105207 0.0015923595052362088 86-87 7.077153356605373E-4 0.0 0.0 1.329089400370489 0.001658707817954384 88-89 7.077153356605373E-4 0.0 0.0 1.4722690592163115 0.0017692883391513432 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3380 0.0 48.043724 1 GTATCAA 6365 0.0 41.206963 1 ATCAACG 7850 0.0 32.91981 3 TCAACGC 7855 0.0 32.898857 4 CAACGCA 7970 0.0 32.305386 5 TATCAAC 8120 0.0 32.00076 2 AACGCAG 8160 0.0 31.551083 6 TACATGG 9480 0.0 27.559713 2 GTACATG 9600 0.0 27.22244 1 ACATGGG 9445 0.0 26.308233 3 ACGCAGA 9935 0.0 25.580671 7 CGCAGAG 10065 0.0 25.156227 8 GAGTACA 7235 0.0 22.837372 1 AGTACAT 6750 0.0 22.578548 2 CATGGGG 5915 0.0 22.48464 4 TACCTGG 2090 0.0 21.740469 2 GTACCTG 2235 0.0 21.606375 1 GCAGAGT 11400 0.0 21.380009 9 GTATACG 115 0.0037963197 20.584078 1 TATAGGG 815 0.0 20.326115 2 >>END_MODULE