FastQCFastQC Report
Thu 26 May 2016
SRR936363_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936363_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260796
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57060.25238898157993905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43060.1904638897096421No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28950.12805224354607847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36950.044.9285551
GTATCAA65300.040.7930561
ATCAACG81900.032.144643
TCAACGC82250.031.9501864
TATCAAC82800.031.9133472
CAACGCA83700.031.283345
AACGCAG87150.030.044936
TACATGG95350.026.4690572
GTACATG99100.026.207791
ACATGGG95250.025.5975063
ACGCAGA103750.025.2377417
CGCAGAG105700.024.7721448
TTAGACG1652.4606834E-622.998824
GAGTACA72450.022.1260031
TATAGGG8250.021.276262
GCAGAGT122350.021.2071789
AGTACAT69350.020.9325682
CATGGGG59450.020.5059914
CATGGGA36850.020.4672344
GTACCTG21700.020.3861221
Produced by FastQC (version 0.11.5)