##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936363_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2260796 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49335057209939 34.0 31.0 34.0 31.0 34.0 2 32.62439291293863 34.0 31.0 34.0 31.0 34.0 3 32.70913032400977 34.0 31.0 34.0 31.0 34.0 4 36.191805894914886 37.0 35.0 37.0 35.0 37.0 5 36.15217295147373 37.0 35.0 37.0 35.0 37.0 6 36.19082349756457 37.0 37.0 37.0 35.0 37.0 7 36.140890642057045 37.0 36.0 37.0 35.0 37.0 8 36.116031698569884 37.0 36.0 37.0 35.0 37.0 9 37.88264752768494 39.0 38.0 39.0 35.0 39.0 10-11 37.81904072724828 39.0 38.0 39.0 35.0 39.0 12-13 37.7619564082739 39.0 38.0 39.0 35.0 39.0 14-15 39.112415715526744 40.5 38.5 41.0 36.0 41.0 16-17 39.044004191444074 40.0 38.0 41.0 36.0 41.0 18-19 39.066180672648045 40.0 38.5 41.0 36.0 41.0 20-21 39.01868324253935 40.0 38.5 41.0 35.0 41.0 22-23 38.90536275718817 40.0 38.0 41.0 35.0 41.0 24-25 39.054789551998496 40.0 39.0 41.0 35.0 41.0 26-27 38.975012340786165 40.0 38.5 41.0 35.0 41.0 28-29 38.885628999697445 40.0 38.0 41.0 35.0 41.0 30-31 38.76444071026311 40.0 38.0 41.0 35.0 41.0 32-33 38.6032490326416 40.0 38.0 41.0 34.5 41.0 34-35 38.468111673941394 40.0 38.0 41.0 34.0 41.0 36-37 38.32278608950122 40.0 38.0 41.0 34.0 41.0 38-39 38.18126735008377 40.0 38.0 41.0 33.5 41.0 40-41 38.03087474500132 40.0 38.0 41.0 33.0 41.0 42-43 38.04984947779455 40.0 38.0 41.0 33.0 41.0 44-45 37.90619764012321 40.0 37.5 41.0 33.0 41.0 46-47 37.69387905852629 40.0 37.0 41.0 33.0 41.0 48-49 37.44742095262023 40.0 37.0 41.0 32.5 41.0 50-51 37.23442738752192 40.0 36.5 41.0 32.0 41.0 52-53 37.010709502316885 40.0 36.0 41.0 31.5 41.0 54-55 36.80460620949435 39.0 36.0 41.0 31.0 41.0 56-57 36.53451505575912 39.0 35.0 41.0 31.0 41.0 58-59 36.252926624074 39.0 35.0 41.0 30.5 41.0 60-61 35.95992517679613 38.0 35.0 40.0 30.0 41.0 62-63 35.65417445890739 38.0 35.0 40.0 29.5 41.0 64-65 35.2646983628775 37.0 35.0 40.0 29.0 41.0 66-67 34.90975390968491 36.5 34.0 39.5 29.0 41.0 68-69 34.547984205563 36.0 34.0 39.0 28.5 41.0 70-71 34.171497118713944 35.5 34.0 39.0 28.0 40.5 72-73 33.77395992384983 35.0 34.0 37.5 27.5 40.0 74-75 33.35608807694281 35.0 33.0 37.0 27.0 39.0 76-77 32.65176159193488 35.0 32.5 36.0 26.0 39.0 78-79 32.711157707285395 35.0 33.0 36.0 26.0 39.0 80-81 32.56010515765244 35.0 33.0 36.0 26.0 37.5 82-83 32.293636842952665 35.0 33.0 35.5 26.0 37.0 84-85 32.01832606745589 35.0 33.0 35.0 26.0 37.0 86-87 31.625734033499704 35.0 32.0 35.0 25.0 36.0 88-89 31.147525694489904 35.0 32.0 35.0 23.5 36.0 90-91 30.937088751041667 34.5 32.0 35.0 22.0 35.5 92-93 30.659546902949227 34.0 31.0 35.0 20.0 35.0 94-95 30.5024685995552 34.0 31.0 35.0 19.0 35.0 96-97 30.252589795806433 34.0 31.0 35.0 17.0 35.0 98-99 29.805918800280963 34.0 31.0 35.0 2.0 35.0 100-101 26.481096702223464 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21216869762669432 1101 2 0.18070611158167793 1101 3 0.2068781310765786 1101 4 0.18362859050059654 1101 5 0.12163633699281462 1101 6 0.13472020486950953 1101 7 0.11041424832978208 1101 8 0.12186407984047065 1101 9 0.14737358958903002 1101 10-11 0.14542582788576652 1101 12-13 0.20634506003431596 1101 14-15 0.3179450850736103 1101 16-17 0.37854199380339537 1101 18-19 0.2779240230790023 1101 20-21 0.24676399844399555 1101 22-23 0.15421899660569238 1101 24-25 0.12242263595793901 1101 26-27 0.29404537620742843 1101 28-29 0.3130800359031838 1101 30-31 0.2567501983201126 1101 32-33 0.3257733584374307 1101 34-35 0.3029480886990896 1101 36-37 0.3067134724408618 1101 38-39 0.3171523691474576 1101 40-41 0.3833214957651805 1101 42-43 0.4185633930775765 1101 44-45 0.41948729305637755 1101 46-47 0.4231975696901529 1101 48-49 0.5358772281403432 1101 50-51 0.35849569211330135 1101 52-53 0.40128546838035106 1101 54-55 0.4390611139833993 1101 56-57 0.4334079778311448 1101 58-59 0.31943176005596285 1101 60-61 0.31259504633935364 1101 62-63 0.3489991343891887 1101 64-65 0.3856017936541818 1101 66-67 0.27802440398849626 1101 68-69 0.3314454459855938 1101 70-71 0.38040666677385815 1101 72-73 0.24459507901197952 1101 74-75 0.2527628324855513 1101 76-77 0.16425830540894282 1101 78-79 0.24818827166716773 1101 80-81 0.290605704199443 1101 82-83 0.21673351292800902 1101 84-85 0.3805634141616494 1101 86-87 0.29948056201175177 1101 88-89 0.3278709581808812 1101 90-91 0.5315866046538957 1101 92-93 0.49468319952904594 1101 94-95 0.5591163871505955 1101 96-97 0.4469393268476267 1101 98-99 0.5027389356006999 1101 100-101 0.9495894386848498 1102 1 0.0065281676988036 1102 2 0.1390345668868136 1102 3 0.13901315700659467 1102 4 0.02921473964274668 1102 5 0.08690477620309167 1102 6 0.11743695543826504 1102 7 0.20800777199586662 1102 8 0.16150583744940405 1102 9 0.139696627909764 1102 10-11 0.17060014488271946 1102 12-13 0.21739168449153823 1102 14-15 0.2593627349925498 1102 16-17 0.1542033225888204 1102 18-19 0.257417979070226 1102 20-21 0.22609196963779254 1102 22-23 0.15181465605709832 1102 24-25 0.1714807018962219 1102 26-27 0.2375365836275165 1102 28-29 0.2100149466590011 1102 30-31 0.20663439129385353 1102 32-33 0.18183790767070462 1102 34-35 0.07854310266871067 1102 36-37 -0.06826688360773403 1102 38-39 0.1626604698384071 1102 40-41 0.030298726711606605 1102 42-43 -0.0034865073051761897 1102 44-45 0.02028014402918643 1102 46-47 0.1171284588413144 1102 48-49 0.10288551498739196 1102 50-51 -0.005928347231552777 1102 52-53 -0.08981173463737946 1102 54-55 -0.11161513893154762 1102 56-57 -0.031674700791704424 1102 58-59 -0.21423823632638772 1102 60-61 -0.20918165809644051 1102 62-63 -0.13568965795868593 1102 64-65 -0.09807948811232592 1102 66-67 -0.14923206638631115 1102 68-69 -0.1314344733080901 1102 70-71 -0.10821328894946447 1102 72-73 -0.007670962845203633 1102 74-75 0.0961770725309492 1102 76-77 -0.04430838224643452 1102 78-79 -0.027832580980522437 1102 80-81 0.0441930325060369 1102 82-83 0.09426441853262446 1102 84-85 0.07163037340453116 1102 86-87 0.060990994663853826 1102 88-89 0.07020019291581647 1102 90-91 0.29342518358144787 1102 92-93 0.22097598247313854 1102 94-95 0.1316054682777441 1102 96-97 -0.062285551834492026 1102 98-99 0.03702184963455224 1102 100-101 0.3581912745109861 1103 1 -0.06772936976569355 1103 2 0.036312405000430203 1103 3 0.08862223911215494 1103 4 -0.06977637700072137 1103 5 0.017207715066845708 1103 6 0.049035135507722316 1103 7 0.0825775569193894 1103 8 0.1271771276470801 1103 9 0.051235558554772354 1103 10-11 0.10860961677778391 1103 12-13 0.14908191087221212 1103 14-15 0.1362581473080482 1103 16-17 0.15519199632826286 1103 18-19 0.06901718356695596 1103 20-21 0.08111269020066203 1103 22-23 0.19590369805785457 1103 24-25 0.05590223140163175 1103 26-27 0.09025500534009723 1103 28-29 0.16963856599603133 1103 30-31 0.13173126179534478 1103 32-33 0.12660530309228335 1103 34-35 0.09905852651154845 1103 36-37 0.17648248290824853 1103 38-39 0.05874094519293749 1103 40-41 0.10520616691890439 1103 42-43 0.1478017480165832 1103 44-45 0.21257870777708376 1103 46-47 0.22560845981502808 1103 48-49 0.15663815180992202 1103 50-51 0.2229354234671348 1103 52-53 0.1432798270994482 1103 54-55 0.05364602536045737 1103 56-57 -0.020367965207789496 1103 58-59 0.09248923331038128 1103 60-61 0.04096141939970721 1103 62-63 0.06274380511007394 1103 64-65 0.10881653023810856 1103 66-67 -0.03202782475029409 1103 68-69 0.004356657129648056 1103 70-71 -0.15909131693712908 1103 72-73 0.024853842114247016 1103 74-75 0.13797142281458008 1103 76-77 0.0483514442551396 1103 78-79 -0.050625580490866184 1103 80-81 0.030244719932994713 1103 82-83 0.15999968472016235 1103 84-85 0.011417505533081851 1103 86-87 -0.32005309450493513 1103 88-89 -0.18121226153330383 1103 90-91 -0.09733866640892685 1103 92-93 0.06692874674050842 1103 94-95 -0.0060613276256624715 1103 96-97 -0.17669543989045522 1103 98-99 -0.3199637934230388 1103 100-101 -0.09484646929445972 1104 1 0.055093538097118255 1104 2 0.059551669950380415 1104 3 0.020929787694853985 1104 4 0.016693258418001733 1104 5 0.09683108147227415 1104 6 -0.002221745186041346 1104 7 0.023425851032833123 1104 8 0.03726331361277602 1104 9 0.07620457627415078 1104 10-11 0.12735845813536883 1104 12-13 0.15071386327408476 1104 14-15 0.16676614479928986 1104 16-17 0.2273349726805236 1104 18-19 0.2120774689785918 1104 20-21 0.21024068071451296 1104 22-23 0.25856818878798293 1104 24-25 0.18687273704899354 1104 26-27 0.23164745388664443 1104 28-29 0.2217277229519894 1104 30-31 0.19741308849211947 1104 32-33 0.1522693846178882 1104 34-35 0.16000518541257236 1104 36-37 0.1624295189002396 1104 38-39 0.2554898614821468 1104 40-41 0.30491293611539305 1104 42-43 0.277585390248241 1104 44-45 0.2850885571499191 1104 46-47 0.2921116149716596 1104 48-49 0.28732377131199627 1104 50-51 0.46086632288505314 1104 52-53 0.46772172030047443 1104 54-55 0.3848020840932307 1104 56-57 0.42134459707911986 1104 58-59 0.3677126049707482 1104 60-61 0.2909204259620708 1104 62-63 0.3451816568690518 1104 64-65 0.24959030631030288 1104 66-67 0.18589645246363062 1104 68-69 0.3198146620897333 1104 70-71 0.3241387177990376 1104 72-73 0.2704582994754716 1104 74-75 0.3539662106875312 1104 76-77 0.37296655601760875 1104 78-79 0.15253351735182719 1104 80-81 0.2587225569586522 1104 82-83 0.28547694808097646 1104 84-85 0.25751996625844953 1104 86-87 0.3154965119337376 1104 88-89 0.3717081985468731 1104 90-91 0.4931128063624257 1104 92-93 0.4932366563574 1104 94-95 0.5271224343992778 1104 96-97 0.4001862318827527 1104 98-99 0.35104087909899917 1104 100-101 0.47751546310456305 1105 1 -0.07850345148954574 1105 2 0.020056962399557676 1105 3 0.09171823960751624 1105 4 0.03247539935956212 1105 5 -0.052925576154478904 1105 6 0.03488761324412337 1105 7 0.1757050118197867 1105 8 0.13561312899683742 1105 9 0.056977559473487815 1105 10-11 0.1005420154869725 1105 12-13 0.013647489202703866 1105 14-15 0.12946566622124323 1105 16-17 0.1117362693753492 1105 18-19 0.14540811578950041 1105 20-21 0.16292654329087952 1105 22-23 0.03924403299230761 1105 24-25 0.09436775755611393 1105 26-27 0.20234786327495158 1105 28-29 0.1701120860717893 1105 30-31 0.15496650551298075 1105 32-33 0.1275444232425329 1105 34-35 0.10740896784761844 1105 36-37 0.21206584860158273 1105 38-39 0.24636817521329846 1105 40-41 0.2738449539011114 1105 42-43 0.2517714446517303 1105 44-45 0.3481058894614364 1105 46-47 0.4212449711168702 1105 48-49 0.3589679041826841 1105 50-51 0.39466169094334447 1105 52-53 0.44914559603797244 1105 54-55 0.4363500865931158 1105 56-57 0.535339163705352 1105 58-59 0.48169609558347304 1105 60-61 0.5646921431966234 1105 62-63 0.4677961899184595 1105 64-65 0.46510030724351026 1105 66-67 0.3409457949254815 1105 68-69 0.4531142489308593 1105 70-71 0.37077344784123767 1105 72-73 0.3226715697650846 1105 74-75 0.36011881835816695 1105 76-77 0.41845574347183145 1105 78-79 0.3426884024393715 1105 80-81 0.4245443830209368 1105 82-83 0.4944457382315264 1105 84-85 0.49443330281560804 1105 86-87 0.44585482804033205 1105 88-89 0.5508747356006154 1105 90-91 0.5334523545176353 1105 92-93 0.6192781551164153 1105 94-95 0.4165142999864351 1105 96-97 0.4055062532618159 1105 98-99 0.5237630615732591 1105 100-101 0.7976176734998006 1106 1 0.05309985147619756 1106 2 0.07325589257727216 1106 3 0.03316636238708526 1106 4 0.08203229492553987 1106 5 0.04082511482129547 1106 6 0.11260540993727375 1106 7 0.04597507110042898 1106 8 0.05723380055535188 1106 9 0.07218038032428353 1106 10-11 0.040025172374228646 1106 12-13 0.15193244332642308 1106 14-15 0.18698192586839468 1106 16-17 0.05913513195589104 1106 18-19 0.2183901619261519 1106 20-21 0.27350884014174426 1106 22-23 0.09830638619521181 1106 24-25 0.21189388151705657 1106 26-27 0.0783980900503849 1106 28-29 0.17508981916584077 1106 30-31 0.271998398472995 1106 32-33 0.20986947720069082 1106 34-35 0.24942970366405603 1106 36-37 0.2411373711940712 1106 38-39 0.2973196573196759 1106 40-41 0.21838334598186293 1106 42-43 0.18557607799122167 1106 44-45 0.2738664366285093 1106 46-47 0.4373144166079612 1106 48-49 0.340806353256248 1106 50-51 0.437643430230338 1106 52-53 0.39719651412761436 1106 54-55 0.5341162211313417 1106 56-57 0.5131814601320386 1106 58-59 0.6127082617209041 1106 60-61 0.5365256014508617 1106 62-63 0.4537957233264862 1106 64-65 0.5768692774335165 1106 66-67 0.42278699662092123 1106 68-69 0.45809536639606563 1106 70-71 0.3902788197407574 1106 72-73 0.3795802219776263 1106 74-75 0.4473081161643293 1106 76-77 0.31259915687638085 1106 78-79 0.17674456224305146 1106 80-81 0.1434193368242731 1106 82-83 0.2642692410195764 1106 84-85 0.38189495224980874 1106 86-87 0.4725181449316409 1106 88-89 0.5580311012191963 1106 90-91 0.6150284471393022 1106 92-93 0.5768621698740795 1106 94-95 0.46983224721642003 1106 96-97 0.589931016209178 1106 98-99 0.5569294476379518 1106 100-101 0.9717943349665674 1107 1 -0.007534475996578749 1107 2 0.04820513517912417 1107 3 0.006217956961954485 1107 4 0.024586262924891855 1107 5 -0.042076974418705504 1107 6 0.01905114864132429 1107 7 -0.025422245188160275 1107 8 -0.02801371235587169 1107 9 0.038514153760722536 1107 10-11 0.009796163726250029 1107 12-13 0.015035777700695974 1107 14-15 0.07620776045860111 1107 16-17 0.04433777238469361 1107 18-19 -0.022109258599485315 1107 20-21 0.006723304505257488 1107 22-23 0.06597897795955276 1107 24-25 0.021690439031196718 1107 26-27 0.045990832050932795 1107 28-29 0.07578744270399085 1107 30-31 0.00526827121644402 1107 32-33 -0.0049914062390499225 1107 34-35 -0.0062644268918532475 1107 36-37 0.014845750839469929 1107 38-39 0.0612519580084907 1107 40-41 -0.06675126197359305 1107 42-43 -0.14430643251382946 1107 44-45 -0.22928495947282101 1107 46-47 -0.07158497601178482 1107 48-49 -0.16125372319070408 1107 50-51 -0.1889847693366491 1107 52-53 -0.13806302963953954 1107 54-55 -0.06300980433895376 1107 56-57 -0.2727735952478625 1107 58-59 -0.23870093830074524 1107 60-61 -0.23942201235819027 1107 62-63 -0.14839607556885426 1107 64-65 -0.1404345579179278 1107 66-67 -0.35092897646413945 1107 68-69 -0.20554551231676754 1107 70-71 -0.1721180162627789 1107 72-73 -0.14097106648670632 1107 74-75 -0.16143290577976188 1107 76-77 -0.44970257129556757 1107 78-79 -0.2776354761228035 1107 80-81 0.007189114175126576 1107 82-83 0.16419518125351118 1107 84-85 -0.02536019966169789 1107 86-87 0.024351319910117297 1107 88-89 0.03847573982367081 1107 90-91 -0.22693486990294076 1107 92-93 -0.058185849139896106 1107 94-95 0.07386059067908235 1107 96-97 -0.10534650502635046 1107 98-99 -0.06330839787541365 1107 100-101 0.2831694973949901 1108 1 -0.05509168061060166 1108 2 0.005472446815005583 1108 3 -0.004529906820991414 1108 4 0.022837268544485312 1108 5 -0.08801016250875904 1108 6 -0.03852329781575037 1108 7 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68-69 -0.6943166075813636 2316 70-71 -0.6280909602147986 2316 72-73 -0.7065854172242538 2316 74-75 -0.7810556196271179 2316 76-77 -0.6188366894472708 2316 78-79 -0.7152559428925471 2316 80-81 -0.7898574086019394 2316 82-83 -0.872136369930935 2316 84-85 -0.7448518807084454 2316 86-87 -0.9006903134055086 2316 88-89 -0.7861861471888361 2316 90-91 -0.7824664586554988 2316 92-93 -0.718041134818737 2316 94-95 -0.6105303153151347 2316 96-97 -0.6006208783888773 2316 98-99 -0.8912401472736953 2316 100-101 -0.8344057194276715 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 19.0 5 34.0 6 60.0 7 161.0 8 412.0 9 711.0 10 1237.0 11 1890.0 12 2861.0 13 4280.0 14 5623.0 15 7486.0 16 9649.0 17 12993.0 18 12239.0 19 8319.0 20 7225.0 21 7661.0 22 8396.0 23 9223.0 24 10885.0 25 13130.0 26 16303.0 27 20529.0 28 25409.0 29 31976.0 30 39870.0 31 49478.0 32 62800.0 33 81920.0 34 113070.0 35 171434.0 36 299662.0 37 549568.0 38 567244.0 39 106923.0 40 111.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.08232131927011 17.825182517364656 13.200418703261803 23.892077460103433 2 16.034495420636908 19.018491761039623 39.86704760927252 25.079965209050957 3 20.562536380991475 22.981330469445275 28.02720811607947 28.428925033483782 4 13.221316739767763 14.95880212102286 35.18455446665688 36.6353266725525 5 14.495160111748252 36.932213255862095 33.248687630374434 15.32393900201522 6 35.05234439551379 34.162215432086754 16.157141113130066 14.62829905926939 7 30.848780694941073 31.23687409213392 19.114550804230017 18.799794408694993 8 27.61208883950609 35.334015099106686 18.210134837464327 18.8437612239229 9 27.036318181737762 15.929345239464329 18.99353148183206 38.04080509696585 10-11 26.579194254749115 25.72491285122141 25.77500598242472 21.920886911604754 12-13 26.71358378057635 22.5292380491734 26.963274652660978 23.793903517589275 14-15 23.328885047567315 24.092974332934062 24.89273689443895 27.685403725059672 16-17 23.5534807558717 27.323373498665614 25.49265618665766 23.630489558805024 18-19 23.468504013630596 25.962824598061925 27.425716429080733 23.14295495922675 20-21 24.13279399503272 25.15683642258586 27.631983439453823 23.0783861429276 22-23 23.6653088106858 25.04294397389944 27.66580509639421 23.62594211902055 24-25 23.43573620011819 25.351941661683057 27.810601288779267 23.401720849419483 26-27 23.248056664644466 25.651916109750815 27.586106227630385 23.513920997974335 28-29 23.18482322633611 25.821388887905925 27.67159837646368 23.322189509294287 30-31 23.39304892311122 25.538245342741327 27.549969656382867 23.518736077764586 32-33 23.18412482514196 25.410785078043357 27.654983661485893 23.75010643532879 34-35 23.54662748218629 25.64680711212473 27.50746541316434 23.299099992524635 36-37 23.69142794712213 25.46929851430548 27.38431113486874 23.454962403703654 38-39 23.47550692953909 25.602239037859515 27.424962706661493 23.49729132593991 40-41 23.675207456192634 25.5082264803916 27.470669948427055 23.345896114988708 42-43 23.58344845157669 25.530594470166353 27.21970311215149 23.666253966105476 44-45 23.439068926377736 25.715618422950037 27.350597292345473 23.49471535832675 46-47 23.710530466024345 25.976318887888862 26.863293347227053 23.44985729885974 48-49 23.83021982530852 26.000293298007808 26.698391218401067 23.471095658282607 50-51 23.669942414894095 26.21927106049842 26.65673296311598 23.4540535614915 52-53 24.00069184361061 25.986977503802596 26.520480052764135 23.49185059982266 54-55 23.770699369135755 26.002554419643598 26.63357347826808 23.593172732952567 56-57 23.572434094777016 26.349416283032784 26.557181261341757 23.520968360848443 58-59 23.692945938790977 25.967046944138072 26.723119621419595 23.616887495651355 60-61 23.5204780330897 26.047455638890217 26.797069486244474 23.634996841775614 62-63 23.682012883957686 26.064094239374096 26.80244037940619 23.451452497262025 64-65 23.55800427552877 26.142208881652422 26.71389387612113 23.585892966697674 66-67 23.50585811369093 26.375444754856254 26.609831227585328 23.508865903867488 68-69 23.523949086958755 26.044963809207022 26.860251875887965 23.57083522794626 70-71 23.545193594895235 25.95870780596433 26.644861609246856 23.85123698989358 72-73 23.606847863564674 26.262565721713038 26.46263087669276 23.66795553802952 74-75 23.475160961006655 26.3782756161989 26.38431331265625 23.7622501101382 76-77 23.72967367072787 26.278990918801053 26.338402081274292 23.652933329196784 78-79 23.673841342953097 26.360088498768896 26.36084126524103 23.605228893036976 80-81 23.58765183959122 26.3762543270579 26.326404143005888 23.709689690344987 82-83 23.769659005058397 26.45995038915497 26.275457847589962 23.494932758196672 84-85 23.717640366713574 26.470778825128544 26.061186309967322 23.75039449819056 86-87 23.610197373884148 26.379561141882313 26.361491842131894 23.648749642101645 88-89 23.685042453150253 26.4001530604867 26.118882169009478 23.795922317353565 90-91 23.508317402396063 26.58394041722232 26.204903541382464 23.702838638999157 92-93 23.54643644414988 26.485970205401816 26.261253048243542 23.70634030220476 94-95 23.605779161983474 26.335899303901417 26.335656021051484 23.722665513063625 96-97 23.759179823353712 26.1560357096649 26.226521713648122 23.858262753333268 98-99 23.35966329497187 26.329696237834423 26.118393042962847 24.192247424230864 100-101 23.42088204532994 26.36951104696411 26.254993023424 23.954613884281947 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 112.0 1 77.0 2 134.0 3 275.5 4 247.0 5 212.5 6 405.0 7 841.0 8 1381.0 9 1623.0 10 1433.0 11 1243.0 12 1323.5 13 1450.0 14 1545.5 15 1702.0 16 1857.0 17 1948.5 18 2118.0 19 2326.0 20 2605.5 21 3043.5 22 3680.0 23 4571.5 24 5627.0 25 6939.5 26 8890.5 27 11621.0 28 14060.5 29 16371.5 30 19103.5 31 22482.5 32 27237.0 33 30573.0 34 32245.0 35 34861.5 36 39210.0 37 44083.0 38 48037.5 39 51666.0 40 55357.0 41 58816.0 42 61329.5 43 64544.0 44 70230.0 45 74811.0 46 77353.5 47 80957.5 48 84129.5 49 86010.5 50 85787.5 51 85660.0 52 88459.0 53 90684.5 54 91978.0 55 88836.0 56 83009.0 57 77546.0 58 72835.5 59 67353.0 60 58067.0 61 48567.5 62 40126.0 63 31200.0 64 22697.0 65 16632.0 66 11867.5 67 9035.5 68 7055.5 69 5504.0 70 4303.5 71 2902.0 72 2221.5 73 1872.5 74 1384.0 75 967.0 76 670.5 77 408.0 78 248.0 79 125.5 80 62.0 81 35.5 82 21.0 83 13.5 84 6.0 85 3.0 86 2.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2749474079041187 2 0.01937370731370721 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.096254593514851E-4 12-13 2.211610423939179E-5 14-15 0.0 16-17 6.855992314211455E-4 18-19 0.0 20-21 4.423220847878358E-5 22-23 6.634831271817537E-5 24-25 0.0023000748408967464 26-27 0.0024991197790512724 28-29 0.0023000748408967464 30-31 0.0010615730034908059 32-33 0.0014817789840392498 34-35 0.0016365917137149922 36-37 6.634831271817537E-5 38-39 1.5481272967574254E-4 40-41 7.740636483787127E-4 42-43 0.0034058800528663355 44-45 0.004157827597005656 46-47 0.013380243064832034 48-49 0.01311484981395933 50-51 0.006280973603987269 52-53 0.007497359337153817 54-55 3.759737720696604E-4 56-57 0.003029906280796675 58-59 0.0015260111925180335 60-61 0.0014154306713210745 62-63 0.0 64-65 0.0010173407950120224 66-67 0.0 68-69 0.0 70-71 2.4327714663330968E-4 72-73 0.0014375467755604664 74-75 0.0 76-77 0.19842568723582316 78-79 0.1087891167535682 80-81 9.731085865332387E-4 82-83 0.0 84-85 5.529026059847948E-4 86-87 0.12484540843136664 88-89 0.5891066686246791 90-91 0.3121688113390151 92-93 0.2932153100058563 94-95 0.0023443070493755296 96-97 0.0028087452384027572 98-99 0.0028087452384027572 100-101 7.29831439899929E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2260796.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.709067297878974 #Duplication Level Percentage of deduplicated Percentage of total 1 70.2416986232403 33.511659267335034 2 15.089699343340978 14.398309629524297 3 5.786921758268993 8.282659188384466 4 2.857616563052608 5.453368836728419 5 1.6119460976170845 3.8452222425883433 6 0.9924113206022058 2.8408211089072553 7 0.6534565496469545 2.1823061752342445 8 0.460914667732872 1.7591847121157693 9 0.3558966332374923 1.5281546783594466 >10 1.7597180919662474 14.950655787293327 >50 0.12327940940624127 4.096536447513618 >100 0.0630102750057693 5.363537333532645 >500 0.002503459616654582 0.8186350029322517 >1k 8.344865388847659E-4 0.7165378675832423 >5k 9.272072654275176E-5 0.25241172196752565 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5706 0.25238898157993905 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4306 0.1904638897096421 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2895 0.12805224354607847 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.423220847878358E-5 2 0.0 0.0 0.0 0.0 4.423220847878358E-5 3 0.0 0.0 0.0 0.0 8.846441695756716E-5 4 0.0 0.0 0.0 0.0 8.846441695756716E-5 5 0.0 0.0 0.0 0.0 8.846441695756716E-5 6 0.0 0.0 0.0 0.0 1.3269662543635073E-4 7 0.0 0.0 0.0 0.0 1.3269662543635073E-4 8 0.0 0.0 0.0 0.0 1.3269662543635073E-4 9 0.0 0.0 0.0 0.0 1.3269662543635073E-4 10-11 0.0 0.0 0.0 0.0 1.3269662543635073E-4 12-13 2.211610423939179E-5 0.0 0.0 0.0 1.9904493815452612E-4 14-15 4.423220847878358E-5 0.0 0.0 4.423220847878358E-5 2.6539325087270146E-4 16-17 4.423220847878358E-5 0.0 0.0 2.211610423939179E-4 3.096254593514851E-4 18-19 8.846441695756716E-5 0.0 0.0 3.5385766783026864E-4 4.423220847878358E-4 20-21 1.3269662543635073E-4 0.0 0.0 4.6443818902722753E-4 5.307865017454029E-4 22-23 1.3269662543635073E-4 0.0 0.0 9.067602738150634E-4 5.307865017454029E-4 24-25 1.7692883391513432E-4 0.0 0.0 0.0013933145670816827 5.307865017454029E-4 26-27 1.7692883391513432E-4 0.0 0.0 0.0017471722349119514 5.307865017454029E-4 28-29 1.7692883391513432E-4 0.0 0.0 0.0021673782154603954 5.307865017454029E-4 30-31 1.7692883391513432E-4 0.0 0.0 0.0036049249910208615 5.307865017454029E-4 32-33 1.9904493815452612E-4 0.0 0.0 0.0063252058124660514 5.307865017454029E-4 34-35 2.6539325087270146E-4 0.0 0.0 0.009089718842390026 6.192509187029702E-4 36-37 3.096254593514851E-4 0.0 0.0 0.015790898426925735 6.192509187029702E-4 38-39 3.5385766783026864E-4 0.0 0.0 0.026362396253355014 6.855992314211455E-4 40-41 3.5385766783026864E-4 0.0 0.0 0.03821662812566901 7.077153356605373E-4 42-43 3.759737720696604E-4 0.0 0.0 0.05022567272765875 7.077153356605373E-4 44-45 4.423220847878358E-4 0.0 0.0 0.06566271348675423 7.077153356605373E-4 46-47 4.644381890272276E-4 0.0 0.0 0.08366522233761914 7.740636483787127E-4 48-49 5.307865017454029E-4 0.0 0.0 0.10403415434209898 8.846441695756716E-4 50-51 5.307865017454029E-4 0.0 0.0 0.12756568925281184 8.846441695756716E-4 52-53 5.307865017454029E-4 0.0 0.0 0.15127415299743985 9.731085865332387E-4 54-55 5.307865017454029E-4 0.0 0.0 0.17686248560241613 0.0010173407950120224 56-57 5.307865017454029E-4 0.0 0.0 0.2071394323061435 0.0010173407950120224 58-59 5.307865017454029E-4 0.0 0.0 0.241375161668722 0.0010173407950120224 60-61 5.307865017454029E-4 0.0 0.0 0.28222360619887865 0.0010173407950120224 62-63 5.307865017454029E-4 0.0 0.0 0.3263452341564652 0.0010615730034908059 64-65 5.307865017454029E-4 0.0 0.0 0.3708870680946003 0.0011058052119695895 66-67 5.307865017454029E-4 0.0 0.0 0.42443015645816784 0.0011058052119695895 68-69 5.307865017454029E-4 0.0 0.0 0.4844753794681166 0.0011058052119695895 70-71 5.307865017454029E-4 0.0 0.0 0.5526814449424008 0.0011058052119695895 72-73 5.529026059847948E-4 0.0 0.0 0.622745263172794 0.0011500374204483732 74-75 5.750187102241865E-4 0.0 0.0 0.6941360476575507 0.0011942696289271566 76-77 5.750187102241865E-4 0.0 0.0 0.7736655585024036 0.0011942696289271566 78-79 5.750187102241865E-4 0.0 0.0 0.8687205745233095 0.0012385018374059403 80-81 6.192509187029702E-4 0.0 0.0 0.9775096912768777 0.0012385018374059403 82-83 7.519475441393208E-4 0.0 0.0 1.0947029276414146 0.0013269662543635074 84-85 7.961797526181044E-4 0.0 0.0 1.211343261399967 0.0013269662543635074 86-87 7.961797526181044E-4 0.0 0.0 1.3372723589390638 0.001371198462842291 88-89 7.961797526181044E-4 0.0 0.0 1.48177898403925 0.0014154306713210745 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3695 0.0 44.928555 1 GTATCAA 6530 0.0 40.793056 1 ATCAACG 8190 0.0 32.14464 3 TCAACGC 8225 0.0 31.950186 4 TATCAAC 8280 0.0 31.913347 2 CAACGCA 8370 0.0 31.28334 5 AACGCAG 8715 0.0 30.04493 6 TACATGG 9535 0.0 26.469057 2 GTACATG 9910 0.0 26.20779 1 ACATGGG 9525 0.0 25.597506 3 ACGCAGA 10375 0.0 25.237741 7 CGCAGAG 10570 0.0 24.772144 8 TTAGACG 165 2.4606834E-6 22.99882 4 GAGTACA 7245 0.0 22.126003 1 TATAGGG 825 0.0 21.27626 2 GCAGAGT 12235 0.0 21.207178 9 AGTACAT 6935 0.0 20.932568 2 CATGGGG 5945 0.0 20.505991 4 CATGGGA 3685 0.0 20.467234 4 GTACCTG 2170 0.0 20.386122 1 >>END_MODULE