##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936362_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 731276 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00998528599325 33.0 31.0 34.0 30.0 34.0 2 32.08051542782752 33.0 31.0 34.0 30.0 34.0 3 32.13720401052407 34.0 31.0 34.0 30.0 34.0 4 35.64248108785192 37.0 35.0 37.0 35.0 37.0 5 35.68799741821145 37.0 35.0 37.0 35.0 37.0 6 35.82126310722627 37.0 35.0 37.0 35.0 37.0 7 35.77970834541268 37.0 35.0 37.0 35.0 37.0 8 35.791196483954074 37.0 35.0 37.0 35.0 37.0 9 37.48172646169162 39.0 38.0 39.0 35.0 39.0 10-11 37.43029102554986 39.0 37.0 39.0 35.0 39.0 12-13 37.391765899605616 39.0 37.0 39.0 35.0 39.0 14-15 38.590508234920875 40.0 38.0 41.0 34.5 41.0 16-17 38.59066070813209 40.0 38.0 41.0 34.5 41.0 18-19 38.67627202314858 40.0 38.5 41.0 35.0 41.0 20-21 38.63195359891478 40.0 38.0 41.0 35.0 41.0 22-23 38.46747876314825 40.0 38.0 41.0 34.0 41.0 24-25 38.609593368304175 40.0 38.0 41.0 35.0 41.0 26-27 38.492591716397094 40.0 38.0 41.0 34.5 41.0 28-29 38.36721908007374 40.0 38.0 41.0 34.0 41.0 30-31 38.17920183350746 40.0 38.0 41.0 34.0 41.0 32-33 37.91494456265487 40.0 37.0 41.0 34.0 41.0 34-35 37.632620241878584 40.0 37.0 41.0 33.0 41.0 36-37 37.41376101499297 40.0 36.5 41.0 33.0 41.0 38-39 37.23113639720161 40.0 36.0 41.0 33.0 41.0 40-41 37.099936959506394 40.0 36.0 41.0 33.0 41.0 42-43 36.92098332230239 40.0 36.0 41.0 32.0 41.0 44-45 36.701631121491744 40.0 36.0 41.0 31.0 41.0 46-47 36.36176218007975 40.0 36.0 41.0 30.5 41.0 48-49 36.12465608060431 40.0 35.5 41.0 30.0 41.0 50-51 35.76368293229916 39.5 35.5 40.5 29.0 41.0 52-53 35.82209179571051 39.5 35.5 40.5 29.0 41.0 54-55 35.97882947067865 40.0 36.0 41.0 29.0 41.0 56-57 35.88527860889732 40.0 36.0 41.0 28.0 41.0 58-59 35.72964858685366 40.0 35.0 41.0 28.0 41.0 60-61 35.493298699806914 39.0 35.0 41.0 27.5 41.0 62-63 35.216815538866314 39.0 35.0 41.0 26.5 41.0 64-65 34.9370470246528 39.0 35.0 40.5 26.0 41.0 66-67 34.61031402644145 38.0 35.0 40.0 26.0 41.0 68-69 34.22416980729574 37.5 35.0 40.0 25.5 41.0 70-71 33.79655834459219 37.0 34.0 39.5 23.5 41.0 72-73 33.35146784524585 36.5 34.0 39.0 22.0 41.0 74-75 32.90648811119195 36.0 34.0 39.0 20.5 40.0 76-77 32.4786831510948 35.5 33.5 37.5 19.0 40.0 78-79 32.04302889743407 35.0 33.0 37.0 15.5 39.0 80-81 31.58801738331355 35.0 33.0 37.0 9.5 39.0 82-83 31.18966915364377 35.0 32.5 36.0 4.5 38.5 84-85 30.79189389505467 35.0 32.0 36.0 2.0 37.0 86-87 30.474725684967098 35.0 32.0 35.5 2.0 37.0 88-89 30.085114922409595 35.0 31.5 35.0 2.0 36.0 90-91 29.682051646710683 34.5 31.0 35.0 2.0 36.0 92-93 29.384095744971802 34.0 31.0 35.0 2.0 36.0 94-95 29.04852750534682 34.0 30.5 35.0 2.0 35.5 96-97 28.652043141030198 34.0 30.0 35.0 2.0 35.0 98-99 28.2581262067947 34.0 30.0 35.0 2.0 35.0 100-101 25.194055322477425 30.5 23.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4008882379832457 1101 2 0.4082034216149282 1101 3 0.434370610722695 1101 4 0.34202290732410034 1101 5 0.5474517255328948 1101 6 0.29969481509620266 1101 7 0.46911559949765547 1101 8 0.36211887156270706 1101 9 0.5301510595580936 1101 10-11 0.5185414517981854 1101 12-13 0.36315347856488955 1101 14-15 0.6302527186408824 1101 16-17 0.6865129096196583 1101 18-19 0.7900145425971772 1101 20-21 0.8232123266675444 1101 22-23 0.8110239504296644 1101 24-25 0.7557998945482964 1101 26-27 0.7934333154524253 1101 28-29 0.6527889300451761 1101 30-31 0.7156778957018446 1101 32-33 0.8138911326383678 1101 34-35 0.6906614917400091 1101 36-37 0.6639768838898732 1101 38-39 0.8395308731712134 1101 40-41 0.7921150062531126 1101 42-43 0.9649812112445559 1101 44-45 1.136332054202441 1101 46-47 1.00059328061716 1101 48-49 1.0516559932956326 1101 50-51 1.027830041120474 1101 52-53 0.9430717774227659 1101 54-55 0.9301990497778618 1101 56-57 0.9004648236319497 1101 58-59 0.9065076585010488 1101 60-61 1.043470413173857 1101 62-63 1.1273015583843318 1101 64-65 1.0373702802986458 1101 66-67 1.165024600706424 1101 68-69 0.8646947915188719 1101 70-71 0.9458155730746753 1101 72-73 0.9503529365900505 1101 74-75 0.7880462828713775 1101 76-77 0.5340969892390532 1101 78-79 0.7309211589818503 1101 80-81 0.9039162492667892 1101 82-83 1.0041509263223318 1101 84-85 1.0687291902046603 1101 86-87 0.9442364468982554 1101 88-89 0.9149092026477312 1101 90-91 0.8296945218644218 1101 92-93 0.8156481086048935 1101 94-95 0.9818970642412843 1101 96-97 1.179710236571676 1101 98-99 1.334721218444372 1101 100-101 1.556175417014746 1102 1 0.41569883016262565 1102 2 0.261275615108147 1102 3 0.2984530245017538 1102 4 0.3840387331584907 1102 5 0.4156901174326677 1102 6 0.35792194160787005 1102 7 0.28317426095238574 1102 8 0.277970494185773 1102 9 0.321282620289395 1102 10-11 0.45417401012710457 1102 12-13 0.5138409386949192 1102 14-15 0.5576235399283931 1102 16-17 0.6063589945579722 1102 18-19 0.5113482016766753 1102 20-21 0.5292074133295941 1102 22-23 0.44432199459541977 1102 24-25 0.5637928910058108 1102 26-27 0.4343619634701241 1102 28-29 0.45406476918086014 1102 30-31 0.3075070961307418 1102 32-33 0.25745696729504886 1102 34-35 0.24210359714604834 1102 36-37 0.0850662333476393 1102 38-39 0.27031117095749835 1102 40-41 0.08422652002267483 1102 42-43 0.3223505395288271 1102 44-45 0.392608775567318 1102 46-47 0.40743802185254907 1102 48-49 0.42477514852725307 1102 50-51 0.5016481933253729 1102 52-53 0.4864299489009696 1102 54-55 0.3758388016678609 1102 56-57 0.27678714945954397 1102 58-59 0.3175898143977278 1102 60-61 0.4144477873883332 1102 62-63 0.3503413129888884 1102 64-65 0.21494129974840348 1102 66-67 0.2905495270742122 1102 68-69 0.21027075145210006 1102 70-71 0.22419824337884364 1102 72-73 0.4478229747294904 1102 74-75 0.6289898609215143 1102 76-77 0.7092384852283047 1102 78-79 0.735968867221878 1102 80-81 0.468249759045964 1102 82-83 0.5599987485735056 1102 84-85 0.6281032146627687 1102 86-87 0.5574834574985736 1102 88-89 0.492303776259579 1102 90-91 0.5091155080609262 1102 92-93 0.4673969583137598 1102 94-95 0.6793969624456082 1102 96-97 0.8566881265509103 1102 98-99 0.9969889546442694 1102 100-101 1.0773407739103895 1103 1 0.45308962953645704 1103 2 0.3033677223646265 1103 3 0.40025578165976583 1103 4 0.37548447370284777 1103 5 0.23791149803157197 1103 6 0.14571924504674172 1103 7 0.1845730389153175 1103 8 0.3796066099258155 1103 9 0.310776636347569 1103 10-11 0.44659182560960886 1103 12-13 0.4723611124059701 1103 14-15 0.560036053183957 1103 16-17 0.49476605409440566 1103 18-19 0.4481481814777126 1103 20-21 0.604308408128361 1103 22-23 0.6485650385786457 1103 24-25 0.7076347331509396 1103 26-27 0.5729558633842728 1103 28-29 0.46192216452500645 1103 30-31 0.5072028492994534 1103 32-33 0.4205181196457062 1103 34-35 0.43679505803580554 1103 36-37 0.6434652133001677 1103 38-39 0.8134094388966986 1103 40-41 0.7497708819880344 1103 42-43 0.49620172369283466 1103 44-45 0.5981874757642558 1103 46-47 0.7619411274426184 1103 48-49 0.9359312864861451 1103 50-51 1.1111391795395846 1103 52-53 1.1502182638027492 1103 54-55 1.1693372614971835 1103 56-57 1.1198185083445722 1103 58-59 1.1696286468978556 1103 60-61 1.081057644480694 1103 62-63 1.0002756663696957 1103 64-65 0.8660560298398039 1103 66-67 1.1853470325026834 1103 68-69 0.9245564657378083 1103 70-71 0.8107457361829589 1103 72-73 0.7560578381340264 1103 74-75 0.9305262442977735 1103 76-77 0.6106246388413936 1103 78-79 0.7795620096750397 1103 80-81 0.7313550966210762 1103 82-83 1.1490546996920301 1103 84-85 1.066950864008831 1103 86-87 1.0605612739910235 1103 88-89 0.8932569146729499 1103 90-91 1.1205986166808337 1103 92-93 1.1826560099726002 1103 94-95 1.2301316996066447 1103 96-97 1.242391050350335 1103 98-99 1.1988283224168335 1103 100-101 1.205510748156712 1104 1 0.12117191571012143 1104 2 -0.07581333975674909 1104 3 0.05229570930764993 1104 4 0.11937139002897368 1104 5 0.03034180169989753 1104 6 0.1612591583390497 1104 7 0.02442220964142905 1104 8 -0.14265410651323407 1104 9 0.15508321407344994 1104 10-11 0.0983470342918622 1104 12-13 0.16974432977813336 1104 14-15 0.328986612572983 1104 16-17 0.2504831630723814 1104 18-19 -0.07535429246691905 1104 20-21 0.09144700644971238 1104 22-23 0.17975612089353632 1104 24-25 0.21022830759418554 1104 26-27 0.17609471053565073 1104 28-29 0.1615747595350001 1104 30-31 0.13408582262679403 1104 32-33 0.2458681564916887 1104 34-35 -0.012454384489238635 1104 36-37 0.0925687074478887 1104 38-39 0.14004071108803373 1104 40-41 0.11736818888158496 1104 42-43 0.3186124559230237 1104 44-45 0.08331868574808254 1104 46-47 0.17600504804669725 1104 48-49 0.2859023360703148 1104 50-51 0.3692117996239901 1104 52-53 0.31116343506480604 1104 54-55 0.41685374648603357 1104 56-57 0.3706989838013115 1104 58-59 0.26107009049479046 1104 60-61 0.43836200187716656 1104 62-63 0.4119194757658917 1104 64-65 0.24396781959757874 1104 66-67 0.38371599957098823 1104 68-69 0.3720581166292902 1104 70-71 0.30562557999427753 1104 72-73 0.5152801007645422 1104 74-75 0.27273571132300845 1104 76-77 0.29070340311323406 1104 78-79 0.3151100099430124 1104 80-81 0.43485379026347104 1104 82-83 0.37706315034233384 1104 84-85 0.3679155588570886 1104 86-87 0.3393968451994773 1104 88-89 0.577648333865401 1104 90-91 0.6506879083766108 1104 92-93 0.6298119306915275 1104 94-95 0.4565813737350233 1104 96-97 0.9110344003680417 1104 98-99 1.0118911807081368 1104 100-101 1.0543297625984245 1105 1 0.11044186809074574 1105 2 -0.008753447173425855 1105 3 0.13518921991801136 1105 4 0.10274527768999775 1105 5 -0.062101149167780534 1105 6 -0.00311252846533705 1105 7 0.055215610325156206 1105 8 0.18111584238133815 1105 9 0.07793935427754661 1105 10-11 0.22390509205331455 1105 12-13 0.1718882476747936 1105 14-15 0.10662107515260999 1105 16-17 0.11987847364417092 1105 18-19 0.10823596240948063 1105 20-21 0.28437056677108075 1105 22-23 0.32295937825431764 1105 24-25 0.2567995495791848 1105 26-27 0.18436012408520952 1105 28-29 0.2457581069287187 1105 30-31 0.15067010242143652 1105 32-33 0.06905244976571367 1105 34-35 -0.09584291354078545 1105 36-37 0.01899920616153139 1105 38-39 0.23126470809467747 1105 40-41 0.2756660226529988 1105 42-43 0.17105745193731536 1105 44-45 0.33533768701932587 1105 46-47 0.5923550655931251 1105 48-49 0.5891720142153645 1105 50-51 0.5166575741884074 1105 52-53 0.5986692160900233 1105 54-55 0.5987869678011108 1105 56-57 0.816381749025453 1105 58-59 0.9076571358854224 1105 60-61 0.7238691168957487 1105 62-63 0.6305863190588568 1105 64-65 0.6072416345164626 1105 66-67 0.7107128644542016 1105 68-69 0.873920826866879 1105 70-71 0.8739021024083868 1105 72-73 0.7569536814211801 1105 74-75 0.8901898756365938 1105 76-77 0.7450292368009812 1105 78-79 0.8431645409885746 1105 80-81 0.9872653202422974 1105 82-83 0.9225304779631998 1105 84-85 0.9210068676812 1105 86-87 0.945876938718456 1105 88-89 0.967534619065745 1105 90-91 0.9705325701953491 1105 92-93 0.8515488179319028 1105 94-95 0.8044457061457173 1105 96-97 0.8220414723920619 1105 98-99 1.2143269968029742 1105 100-101 1.2267135436561851 1106 1 0.008126579257634603 1106 2 0.18347607798868637 1106 3 0.23795809838460968 1106 4 0.038575555898979985 1106 5 0.21653279739231834 1106 6 0.21243502688470528 1106 7 0.259769121791507 1106 8 0.23250138256035058 1106 9 0.26965363684627874 1106 10-11 0.16193616074131967 1106 12-13 0.23722827475805985 1106 14-15 0.27492815093070533 1106 16-17 0.23940154396156998 1106 18-19 0.17124969120251166 1106 20-21 0.1655554045467369 1106 22-23 0.12782445735727066 1106 24-25 0.04355076887656395 1106 26-27 0.09833442801228642 1106 28-29 0.2198946902537955 1106 30-31 0.18537191127524721 1106 32-33 0.3597120263419953 1106 34-35 0.08407143288280139 1106 36-37 0.1670257609721162 1106 38-39 0.1846011240760035 1106 40-41 -0.0032519679008871094 1106 42-43 0.18307097155066288 1106 44-45 0.3959557579422537 1106 46-47 0.3198467809766399 1106 48-49 0.3826114787153756 1106 50-51 0.27173898912383976 1106 52-53 0.19418616514926867 1106 54-55 0.2706996407872069 1106 56-57 0.3921334197105679 1106 58-59 0.3671951790645167 1106 60-61 0.43361578928733024 1106 62-63 0.24186110409920758 1106 64-65 0.2365245885739924 1106 66-67 0.25334698716560666 1106 68-69 -0.026225598912859027 1106 70-71 0.1753465431830037 1106 72-73 0.15420241808597268 1106 74-75 0.20770922475748677 1106 76-77 0.2629504311068942 1106 78-79 0.30829098460363724 1106 80-81 0.33672640731609604 1106 82-83 0.4547705722041542 1106 84-85 0.5161098611525929 1106 86-87 0.33079067962189157 1106 88-89 0.4185040017654842 1106 90-91 0.40137116054446054 1106 92-93 0.2147353952017781 1106 94-95 0.3145402773824095 1106 96-97 0.5330278096557706 1106 98-99 0.5321975476061134 1106 100-101 0.6639706475083926 1107 1 -0.09780545593763534 1107 2 0.01708579858762249 1107 3 0.21570304292338704 1107 4 0.17792766298434515 1107 5 0.13222292022293658 1107 6 -0.013937169732486154 1107 7 0.04744857136920899 1107 8 0.16599643184696333 1107 9 0.1966344735058101 1107 10-11 0.16224268598003277 1107 12-13 0.17049728924634167 1107 14-15 0.36604222683429555 1107 16-17 0.012224442940556912 1107 18-19 0.2884636193565271 1107 20-21 0.2933576847416717 1107 22-23 0.21317960764285004 1107 24-25 0.3752474632554623 1107 26-27 0.37805193964481276 1107 28-29 0.4083999938019929 1107 30-31 0.29549496241423867 1107 32-33 0.08040909560787668 1107 34-35 0.07346213127519263 1107 36-37 0.21005947874837005 1107 38-39 0.27585853912000147 1107 40-41 0.6274964829337861 1107 42-43 0.33287911762427314 1107 44-45 0.6443514621784701 1107 46-47 0.768314154783063 1107 48-49 0.5547717098221696 1107 50-51 0.8562462332634695 1107 52-53 0.8221703757916501 1107 54-55 0.7824755024759611 1107 56-57 0.8446782407571689 1107 58-59 1.061235578649935 1107 60-61 1.0664139898298188 1107 62-63 1.3305847241843267 1107 64-65 1.1632661591299254 1107 66-67 0.9809199247358933 1107 68-69 1.1823251321167518 1107 70-71 1.0615573178759306 1107 72-73 0.9898144762154999 1107 74-75 1.0160824357747558 1107 76-77 1.0520687851325192 1107 78-79 1.0757166136419372 1107 80-81 1.130228733820605 1107 82-83 1.1599594490868554 1107 84-85 0.9697815483645371 1107 86-87 0.7019078428300425 1107 88-89 0.8310395608983931 1107 90-91 0.9346478932941444 1107 92-93 0.5506848516156531 1107 94-95 0.46378278515580007 1107 96-97 0.5374338343101037 1107 98-99 0.6638435219814873 1107 100-101 0.6474756202739549 1108 1 0.05232334938231986 1108 2 -0.08742195968629574 1108 3 -0.08422506573523947 1108 4 -0.11983514694008335 1108 5 0.07015244959140432 1108 6 0.09056203004841734 1108 7 0.044020894914474695 1108 8 -0.03929614023694228 1108 9 -0.008144588996863433 1108 10-11 -0.07385404210808844 1108 12-13 -0.14809047382033924 1108 14-15 -0.3093598083753406 1108 16-17 0.01786662946435058 1108 18-19 -0.018336959197604585 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-1.650950224114606 2316 100-101 -1.6880343418660892 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3615.0 3 649.0 4 490.0 5 403.0 6 505.0 7 554.0 8 720.0 9 888.0 10 1186.0 11 1615.0 12 2567.0 13 4216.0 14 4555.0 15 4182.0 16 5320.0 17 7868.0 18 6811.0 19 3647.0 20 3203.0 21 3083.0 22 3375.0 23 3520.0 24 4100.0 25 4648.0 26 5245.0 27 6108.0 28 7143.0 29 8932.0 30 10876.0 31 13515.0 32 17149.0 33 22588.0 34 31876.0 35 55428.0 36 74220.0 37 142699.0 38 213513.0 39 50247.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.519048022962146 21.82649238927302 14.052961794506267 20.601497793258563 2 13.75722448488363 24.30627165977839 40.745547693225056 21.19095616211293 3 18.241159504327765 30.746749561342927 30.852328130534502 20.159762803794813 4 9.935561688196247 18.652375000341884 43.1229333107234 28.28913000073847 5 9.141882642225221 42.86183171343117 36.61607976838613 11.38020587595748 6 24.828748155512674 42.55757056916195 19.165437008450112 13.448244266875264 7 24.690387234764152 36.243975953043886 24.224874048870678 14.84076276332128 8 23.83641339206711 35.444038571735305 22.61528509632677 18.104262939870814 9 26.628609254133067 17.54693808330689 23.594592820333272 32.22985984222677 10-11 22.18620342589579 28.96319199621472 29.91532354011055 18.93528103777894 12-13 24.275091030309245 28.378617706890953 29.208745747645175 18.137545515154624 14-15 21.68466758979093 29.801938560951847 28.885036839496042 19.628357009761185 16-17 17.319926881358644 33.05985648449238 30.386567287682738 19.23364934646624 18-19 16.857052007508283 32.81577696707775 33.39387370630853 16.933297319105435 20-21 18.35234879596087 31.43124005978828 32.554458343076966 17.661952801173886 22-23 18.11512295723729 30.78522639544481 32.37403233956889 18.72561830774901 24-25 18.243173931894557 31.30601766304534 32.872426418794994 17.578381986265114 26-27 17.80530699936955 30.966886982493712 33.709894245667876 17.517911772468864 28-29 17.358919457868854 30.807842150361452 34.243313458291475 17.58992493347822 30-31 17.463305688370514 31.825306082256528 33.461232316159176 17.25015591321378 32-33 16.978581285396405 32.44256225869584 33.232241449814886 17.346615006092865 34-35 17.682585694814296 30.988009993500388 33.53307535987845 17.796328951806867 36-37 18.059137709049285 30.490656934507165 33.126763118010224 18.323442238433326 38-39 17.47090426920593 31.39851876279594 33.839880731549805 17.290696236448326 40-41 16.918908369742542 31.37990321724992 34.52396823018957 17.17722018281797 42-43 16.82628024103505 31.676870117288146 32.69481032575461 18.802039315922194 44-45 17.175079665381347 31.83509042716588 32.47584955217644 18.51398035527633 46-47 17.637372304511818 31.24037816558352 32.70908246187004 18.413167068034618 48-49 17.677167573244475 31.553306637744154 32.45582924720363 18.31369654180774 50-51 17.79680823567583 31.449794603036686 32.37999263100587 18.37340453028162 52-53 17.21009449932677 32.04749915975397 32.45933999619226 18.283066344727 54-55 17.549438134297997 31.304221139784712 32.60786502028654 18.538475705630752 56-57 18.35074655583776 31.949552506294598 31.007630482347636 18.692070455520003 58-59 18.090406335122715 31.013116628597658 32.12151572373719 18.77496131254243 60-61 18.175752515830233 32.230319147108645 31.57477744909073 18.019150887970397 62-63 18.273511403122416 32.06108233981312 32.24708609012146 17.418320166943005 64-65 17.97750260806459 31.384329071089173 32.34756051465808 18.29060780618816 66-67 17.955376925851496 31.73425396334656 31.36098658559626 18.94938252520568 68-69 17.47536918729314 31.858416810529672 32.04356025202599 18.622653750151194 70-71 18.453845117919148 30.967277814270126 32.276811112241 18.302065955569724 72-73 18.05583041977263 30.912329309321194 31.626632693449437 19.405207577456736 74-75 17.684587582068133 32.190570574987234 31.192523097287296 18.93231874565734 76-77 18.052286728351582 30.595191874907663 32.46965287631979 18.88286852042096 78-79 17.172980442629722 30.772777591861782 32.886012573724095 19.168229391784404 80-81 18.140811775660474 31.88135005033655 31.104689717256335 18.873148456746645 82-83 18.539823860231664 30.665100796587154 31.699179773613118 19.09589556956806 84-85 17.35551388151118 30.624535719611412 31.754201630143847 20.265748768733562 86-87 18.049643684243076 31.216009126081488 31.58606898163101 19.14827820804442 88-89 18.556748505261737 30.154982655606393 31.910797512194687 19.37747132693718 90-91 18.01254405992769 29.509065671721828 32.830154693572524 19.648235574777956 92-93 18.493356906782225 31.29677798980439 31.824362224556346 18.385502878857036 94-95 18.00282569735352 30.090034743983026 32.015590812635374 19.891548746028082 96-97 17.993821244778044 29.913103096728367 31.821207680782177 20.271867977711413 98-99 18.0973322452389 30.52503304125142 31.43713644319515 19.94049827031453 100-101 18.07277086417548 29.92254399192122 31.78931965802571 20.21536548587759 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2575.0 1 1566.0 2 1096.0 3 1592.5 4 1085.5 5 1183.5 6 2864.0 7 4753.5 8 5770.0 9 5284.0 10 3730.5 11 2778.5 12 2724.0 13 2700.0 14 2458.5 15 2233.5 16 2119.5 17 1954.5 18 1750.0 19 1690.5 20 1803.0 21 2056.5 22 2324.5 23 2714.0 24 3009.5 25 3554.0 26 5353.5 27 7155.5 28 7535.5 29 8865.5 30 12744.0 31 16351.5 32 21416.0 33 28199.0 34 32743.0 35 36883.5 36 44164.5 37 47790.0 38 43050.5 39 38366.5 40 34672.0 41 30249.5 42 24918.5 43 24254.0 44 25531.0 45 22942.0 46 19164.0 47 19158.5 48 32128.0 49 30690.0 50 16214.5 51 9966.5 52 5746.5 53 4213.0 54 3101.0 55 2464.5 56 2076.5 57 1744.0 58 1423.5 59 1192.5 60 918.5 61 623.0 62 404.5 63 238.5 64 128.5 65 88.0 66 56.5 67 31.0 68 27.5 69 22.5 70 21.5 71 17.5 72 12.5 73 13.0 74 11.0 75 8.5 76 6.5 77 4.5 78 3.5 79 2.0 80 2.5 81 4.0 82 4.5 83 4.0 84 5.5 85 6.5 86 3.5 87 5.0 88 4.5 89 5.0 90 5.5 91 3.0 92 2.0 93 1.5 94 5.0 95 7.5 96 6.0 97 4.0 98 2.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.045673589725356775 2 0.01121327651939897 3 0.00916206739999672 4 0.004649407337311767 5 0.008068089202982184 6 0.005880132808953118 7 0.005469890985072667 8 0.00916206739999672 9 0.01367472746268167 10-11 0.003008440041789967 12-13 0.00868345193880286 14-15 0.00868345193880286 16-17 0.01852925571193366 18-19 0.011418397431339194 20-21 0.003965670964177684 22-23 0.009093693762683309 24-25 0.01982835482088842 26-27 0.007247605555221285 28-29 0.01702503569103868 30-31 0.01367472746268167 32-33 0.012717496540293954 34-35 0.27376804380288705 36-37 0.4809401648625143 38-39 0.4794359448416193 40-41 0.5014522560565368 42-43 0.4896919904386306 44-45 0.5061700370311619 46-47 0.514716741695338 48-49 0.5216908527013057 50-51 0.5338613601430923 52-53 0.5210754899654849 54-55 0.5059649161192218 56-57 0.5015890033311636 58-59 0.4991275523878809 60-61 0.4968712223565384 62-63 0.49263205684310707 64-65 0.5093835979848921 66-67 0.48046154940132046 68-69 0.5103408289072799 70-71 0.48873475951624284 72-73 0.49830706874012004 74-75 0.5135543898610101 76-77 0.49960616784907474 78-79 0.49878568420131386 80-81 0.5023411133416111 82-83 0.5021359924296709 84-85 0.5020676187923575 86-87 0.5047341906475804 88-89 0.5206652481416045 90-91 0.5469207248699534 92-93 0.5338613601430923 94-95 0.5015890033311636 96-97 0.47307719657147235 98-99 0.4646672391819231 100-101 0.47088924017744327 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 731276.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.38082631366515 #Duplication Level Percentage of deduplicated Percentage of total 1 70.3548719256376 14.338904250365763 2 11.495630782241587 4.685809086777769 3 4.950993968118058 3.0271604443265385 4 2.7003295537578333 2.2013979049918126 5 1.7730797562532281 1.806841527623639 6 1.1847163241398824 1.448729857995526 7 0.9240337778226924 1.3182800353634903 8 0.6652434281268836 1.0846568611968963 9 0.5237831900699347 0.9607620800549518 >10 4.0547520286664485 16.833971044032605 >50 0.6631268860557107 9.53824971767114 >100 0.632252557352712 25.389461580685946 >500 0.05302330364210642 7.318932321223564 >1k 0.022820155997868585 8.169780090325137 >5k 0.0013423621175216813 1.877063197365239 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8240 1.1267975429249695 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5482 0.749648559504209 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4482 0.6129012848773924 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4382 0.5992265574147108 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3569 0.4880510231431087 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3052 0.4173526821610445 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2880 0.39383215092523205 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2428 0.33202238279391094 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2036 0.2784174511401988 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2025 0.2769132311193038 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1881 0.2572216235730422 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1866 0.25517041445363997 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1784 0.24395713793424095 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1700 0.23247036686558836 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1687 0.23069265229543975 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 1540 0.2105908029252977 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1489 0.20361669191933002 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1473 0.20142873552530097 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1417 0.19377088814619925 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1343 0.1836515898238148 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1333 0.18228411707754663 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1331 0.182010622528293 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 1320 0.18050640250739802 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1299 0.17763470974023488 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 1292 0.17667747881784715 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1225 0.16751541141785045 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1191 0.16286600408053867 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1168 0.1597208167641219 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1125 0.15384068395516876 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1109 0.1516527275611397 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 1104 0.15096899118800564 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1055 0.14426837473129162 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 1053 0.143994880182038 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 1040 0.14221716561188935 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1039 0.14208041833726254 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1006 0.1375677582745776 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 986 0.13483281278204123 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 982 0.13428582368353398 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 968 0.13237136183875856 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 965 0.1319611200148781 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 953 0.1303201527193563 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 951 0.13004665817010266 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 941 0.1286791854238345 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 905 0.1237562835372691 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 902 0.12334604171338864 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 901 0.12320929443876184 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 890 0.12170507441786685 No Hit GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT 868 0.11869663437607687 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 855 0.11691891980592826 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 850 0.11623518343279418 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 847 0.11582494160891374 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 845 0.1155514470596601 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 822 0.11240625974324332 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 820 0.1121327651939897 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 819 0.11199601791936287 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 814 0.11131228154622878 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 766 0.10474841236414159 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 758 0.10365443416712704 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 747 0.10215021414623207 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 740 0.10119298322384435 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.0512091194022504E-4 0.0 12-13 0.0 0.0 0.0 5.469890985072668E-4 0.0 14-15 0.0 0.0 0.0 8.204836477609001E-4 0.0 16-17 0.0 0.0 0.0 0.0027349454925363337 0.0 18-19 0.0 0.0 0.0 0.004649407337311767 0.0 20-21 0.0 0.0 0.0 0.010256045597011251 0.0 22-23 0.0 0.0 0.0 0.027486202199990158 0.0 24-25 0.0 0.0 0.0 0.04334888605670089 0.0 26-27 0.0 0.0 0.0 0.050528117974608766 0.0 28-29 0.0 0.0 0.0 0.05777572352983005 0.0 30-31 0.0 0.0 0.0 0.07568961650594304 0.0 32-33 0.0 0.0 0.0 0.10290232415667956 0.0 34-35 0.0 0.0 0.0 0.14160180287606866 0.0 36-37 0.0 0.0 0.0 0.20361669191933007 0.0 38-39 0.0 0.0 0.0 0.28463945213571895 0.0 40-41 0.0 0.0 0.0 0.38173001712075877 0.0 42-43 0.0 0.0 0.0 0.4704106247162494 0.0 44-45 0.0 0.0 0.0 0.5603219577833813 0.0 46-47 0.0 0.0 0.0 0.6815484167400544 0.0 48-49 0.0 0.0 0.0 0.8122104376459778 0.0 50-51 0.0 0.0 0.0 0.9408896230698123 0.0 52-53 0.0 0.0 0.0 1.0599964992697695 0.0 54-55 0.0 0.0 0.0 1.183137420071218 0.0 56-57 0.0 0.0 0.0 1.3269271793413158 0.0 58-59 0.0 0.0 0.0 1.4876736006651388 0.0 60-61 0.0 0.0 0.0 1.683632445205367 0.0 62-63 0.0 0.0 0.0 1.8596945612873936 0.0 64-65 0.0 0.0 0.0 2.0239964117515137 0.0 66-67 0.0 0.0 0.0 2.2203654981156227 0.0 68-69 0.0 0.0 0.0 2.4520837549707633 0.0 70-71 0.0 0.0 0.0 2.7142966540676845 0.0 72-73 0.0 0.0 0.0 2.951006186446704 0.0 74-75 0.0 0.0 0.0 3.213902821916759 0.0 76-77 0.0 0.0 0.0 3.485482909325617 0.0 78-79 0.0 0.0 0.0 3.8149070939016188 0.0 80-81 0.0 0.0 0.0 4.169150908822387 0.0 82-83 0.0 0.0 0.0 4.515463381814801 0.0 84-85 0.0 0.0 0.0 4.887757836986308 0.0 86-87 0.0 0.0 0.0 5.281111372450347 0.0 88-89 0.0 0.0 0.0 5.697643570963631 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACAAC 20 1.774783E-5 94.65927 1 CGTCTAG 55 8.3920713E-7 51.63234 1 ACTGTAC 55 0.0026274973 34.414494 3 GATAGAC 55 0.0026283786 34.41214 2 GGGATGC 320 0.0 34.0112 7 GGATGCC 330 0.0 32.98281 8 CATGGGT 400 0.0 31.940952 4 GTATGGT 90 2.4717261E-5 31.553093 1 CTGTACA 90 2.4751955E-5 31.546621 4 GTGCGTG 60 0.004024144 31.54662 9 GGACAAT 395 0.0 31.147295 6 ATGGGTA 370 0.0 30.694008 5 GACAATG 410 0.0 30.00776 7 CCAGACT 80 4.5930894E-4 29.574957 4 CATGGGG 1690 0.0 29.399956 4 ACTGTCC 65 0.0059501543 29.121948 8 TCAATAC 130 3.1369018E-7 29.119957 3 TCTAAGA 130 3.1387754E-7 29.117964 2 ACCTGGG 230 0.0 28.803436 3 AGGCTTA 665 0.0 28.463116 6 >>END_MODULE