##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936362_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 731276 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45740048900825 34.0 31.0 34.0 31.0 34.0 2 32.56815894409225 34.0 31.0 34.0 31.0 34.0 3 32.65479244498657 34.0 31.0 34.0 31.0 34.0 4 36.132586055059924 37.0 35.0 37.0 35.0 37.0 5 36.13361576203786 37.0 35.0 37.0 35.0 37.0 6 36.20051799867629 37.0 37.0 37.0 35.0 37.0 7 36.155807109764304 37.0 36.0 37.0 35.0 37.0 8 36.15052319507272 37.0 36.0 37.0 35.0 37.0 9 37.88810791000935 39.0 38.0 39.0 35.0 39.0 10-11 37.835104666364 39.0 38.0 39.0 35.0 39.0 12-13 37.815124522068274 39.0 38.0 39.0 35.0 39.0 14-15 39.05532726357764 40.0 38.0 41.0 36.0 41.0 16-17 39.021652563464414 40.0 38.0 41.0 35.5 41.0 18-19 39.170937511965384 40.5 38.5 41.0 36.0 41.0 20-21 39.1534277071858 40.0 39.0 41.0 36.0 41.0 22-23 39.008857804713955 40.0 38.5 41.0 35.0 41.0 24-25 39.12476547842401 40.5 39.0 41.0 35.0 41.0 26-27 38.960743959872886 40.5 38.0 41.0 35.0 41.0 28-29 38.853715286704336 40.0 38.0 41.0 35.0 41.0 30-31 38.69429052778978 40.0 38.0 41.0 35.0 41.0 32-33 38.45495271279243 40.0 38.0 41.0 35.0 41.0 34-35 38.287944633763445 40.0 38.0 41.0 34.0 41.0 36-37 38.11783034039132 40.0 37.0 41.0 34.0 41.0 38-39 37.95297466346496 40.0 37.0 41.0 33.5 41.0 40-41 37.84221210596273 40.0 37.0 41.0 33.0 41.0 42-43 37.8020603711868 40.0 37.0 41.0 33.0 41.0 44-45 37.60678868170157 40.0 37.0 41.0 33.0 41.0 46-47 37.31737743341775 40.0 37.0 41.0 33.0 41.0 48-49 37.0424784896537 40.0 37.0 41.0 32.5 41.0 50-51 36.858197178630235 40.0 37.0 41.0 31.5 41.0 52-53 36.701484801907895 40.0 37.0 41.0 31.0 41.0 54-55 36.5956000196916 40.0 36.5 41.0 31.0 41.0 56-57 36.42388509946997 40.0 36.0 41.0 31.0 41.0 58-59 36.24256573441491 40.0 36.0 41.0 30.0 41.0 60-61 36.0211438636028 39.0 35.5 41.0 29.5 41.0 62-63 35.762943813279804 39.0 35.0 41.0 29.0 41.0 64-65 35.425382482127134 39.0 35.0 40.5 28.0 41.0 66-67 35.09359188596371 38.0 35.0 40.0 28.0 41.0 68-69 34.73070837823202 37.5 35.0 40.0 27.5 41.0 70-71 34.34278576077979 37.0 34.5 39.5 26.5 41.0 72-73 33.89513808739792 36.5 34.0 39.0 26.0 41.0 74-75 33.4441271147966 36.0 34.0 39.0 26.0 40.0 76-77 32.63725460701568 35.0 33.0 37.0 25.5 39.0 78-79 32.61669397053917 35.0 33.5 37.0 25.0 39.0 80-81 32.39127497688971 35.0 33.0 37.0 25.0 39.0 82-83 32.05769982879241 35.0 33.0 36.0 24.0 38.5 84-85 31.683922349427576 35.0 33.0 36.0 22.5 37.0 86-87 31.297724115108387 35.0 33.0 35.5 20.0 37.0 88-89 30.737786417166706 35.0 32.0 35.0 13.5 36.5 90-91 30.46007253075446 35.0 32.0 35.0 9.0 36.0 92-93 30.202822873990122 35.0 32.0 35.0 2.0 36.0 94-95 30.060594084859886 34.5 31.5 35.0 2.0 36.0 96-97 29.814923503574576 34.0 31.0 35.0 2.0 35.0 98-99 29.489392513907198 34.0 31.0 35.0 2.0 35.0 100-101 26.525365251970527 31.5 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17365236441852971 1101 2 0.21529164883828855 1101 3 0.2827681011388208 1101 4 0.06798015413185254 1101 5 0.16393273745082126 1101 6 0.21276992838800624 1101 7 0.21307920990106055 1101 8 0.20141081450807263 1101 9 0.289757047311646 1101 10-11 0.23507657086751266 1101 12-13 0.2424530297960743 1101 14-15 0.46292394848968854 1101 16-17 0.5645225033396102 1101 18-19 0.48618403235702345 1101 20-21 0.4497135682681659 1101 22-23 0.5636165116274938 1101 24-25 0.2629578097930718 1101 26-27 0.4363648578230013 1101 28-29 0.45967085954188747 1101 30-31 0.4819079955913921 1101 32-33 0.47361061043955033 1101 34-35 0.4566876049177466 1101 36-37 0.5554914417656747 1101 38-39 0.5891212336321203 1101 40-41 0.5655717218301604 1101 42-43 0.4614621184840928 1101 44-45 0.647298681121903 1101 46-47 0.4540537650659786 1101 48-49 0.5319429096532247 1101 50-51 0.45912087710346583 1101 52-53 0.5688381164263134 1101 54-55 0.3473297334520993 1101 56-57 0.30946070955780414 1101 58-59 0.32069798147394124 1101 60-61 0.4485560883458888 1101 62-63 0.4641458702456731 1101 64-65 0.570237853776014 1101 66-67 0.37127051969053326 1101 68-69 0.48986243652481676 1101 70-71 0.5572340595544958 1101 72-73 0.6289963922957824 1101 74-75 0.4820407583096724 1101 76-77 0.4056190282712393 1101 78-79 0.279194545952663 1101 80-81 0.2773222335724128 1101 82-83 0.29261983490441423 1101 84-85 0.5249767164939989 1101 86-87 0.4945841749236486 1101 88-89 0.546875258333742 1101 90-91 0.5154715335177826 1101 92-93 0.6724533102580281 1101 94-95 0.7197461148300555 1101 96-97 0.6083955424635334 1101 98-99 0.5386442257289339 1101 100-101 0.6599014102090237 1102 1 0.03933636349558611 1102 2 0.1095001535276765 1102 3 0.1438862248409265 1102 4 0.163103462627042 1102 5 0.1493936004066967 1102 6 0.06489202890784895 1102 7 0.1333446930233393 1102 8 0.1285278252441202 1102 9 0.07149354581185463 1102 10-11 0.16261705161456774 1102 12-13 0.24259011463937696 1102 14-15 0.16779928271881772 1102 16-17 0.14964404737641956 1102 18-19 0.38257639360951856 1102 20-21 0.26715998981084965 1102 22-23 0.4048261157101791 1102 24-25 0.2966413202508775 1102 26-27 0.31212934334765663 1102 28-29 0.34712420319051773 1102 30-31 0.060044998998158405 1102 32-33 0.25734094690818665 1102 34-35 0.19632819831867465 1102 36-37 0.28113988022000314 1102 38-39 0.2812368192287167 1102 40-41 0.29438668443722094 1102 42-43 0.34048406563177736 1102 44-45 0.3434589482337529 1102 46-47 0.30946454454939953 1102 48-49 0.5153515717865957 1102 50-51 0.4525353755222383 1102 52-53 0.48704596962337376 1102 54-55 0.2944095548346226 1102 56-57 0.4817302102160781 1102 58-59 0.3508172860058849 1102 60-61 0.18308110972298408 1102 62-63 0.3536188401005802 1102 64-65 0.5254545428377284 1102 66-67 0.44344229650011613 1102 68-69 0.3944350982781444 1102 70-71 0.3396206931036403 1102 72-73 0.2219032696116301 1102 74-75 0.21255638733908455 1102 76-77 0.13856825258819327 1102 78-79 0.2289522722444488 1102 80-81 0.2537341395946413 1102 82-83 0.5304769680667292 1102 84-85 0.6360005095366041 1102 86-87 0.4147098586512712 1102 88-89 0.4646922161360436 1102 90-91 0.4517406541388702 1102 92-93 0.5896472785249252 1102 94-95 0.659892972058227 1102 96-97 0.565240962336766 1102 98-99 0.6081788158995458 1102 100-101 0.8342407562736085 1103 1 -0.012410846523103203 1103 2 0.007626700010533227 1103 3 0.058626415721093394 1103 4 0.006114622552310323 1103 5 -0.005163789887703274 1103 6 0.11877527892047368 1103 7 0.05162222673035188 1103 8 0.08125973455079105 1103 9 0.1576294848109967 1103 10-11 0.1327554144888765 1103 12-13 0.16872378125554377 1103 14-15 0.3017171234500182 1103 16-17 0.2461256410743502 1103 18-19 0.18796572855877258 1103 20-21 0.1750464211596352 1103 22-23 0.21709824619545515 1103 24-25 0.1300668113380894 1103 26-27 0.17717251862412553 1103 28-29 0.10007956348945868 1103 30-31 0.1811453373307259 1103 32-33 0.1947128097572488 1103 34-35 0.15389775848632326 1103 36-37 0.19372130727651182 1103 38-39 0.17096312332405006 1103 40-41 0.10365926939909542 1103 42-43 0.16831696973721222 1103 44-45 0.22001174326481276 1103 46-47 0.2929506325158684 1103 48-49 0.3119152237585183 1103 50-51 0.17804034951607406 1103 52-53 0.34226613828468544 1103 54-55 0.28098987094833916 1103 56-57 0.3462482125389599 1103 58-59 0.4223140289236227 1103 60-61 0.4217684298259954 1103 62-63 0.39724606829832965 1103 64-65 0.3537318240577534 1103 66-67 0.30725207279663636 1103 68-69 0.31799213509074775 1103 70-71 0.3458646965374612 1103 72-73 0.5081402034095461 1103 74-75 0.5647290379475791 1103 76-77 0.537011670252177 1103 78-79 0.3866329266907371 1103 80-81 0.4935197781342566 1103 82-83 0.6312937635017661 1103 84-85 0.6400857491467669 1103 86-87 0.6734446463097044 1103 88-89 0.6740670583316692 1103 90-91 0.8338670996269286 1103 92-93 0.8737191363242474 1103 94-95 0.6244980823950463 1103 96-97 0.7161398160457964 1103 98-99 0.9146020851011798 1103 100-101 1.613399217112871 1104 1 -0.05306345110349042 1104 2 -0.10954352843456405 1104 3 -0.07187535958539826 1104 4 0.04959049082552269 1104 5 -0.10485370940076422 1104 6 -0.23823598542314528 1104 7 -0.019378069951592636 1104 8 -0.03926252138045072 1104 9 -0.030890359383128896 1104 10-11 -0.04286355018682997 1104 12-13 0.15089056436189452 1104 14-15 0.10062777433192593 1104 16-17 0.06572507259345173 1104 18-19 -0.0015793431712651795 1104 20-21 -0.06198929551867849 1104 22-23 0.12729094590547874 1104 24-25 0.11241543176704027 1104 26-27 0.24287082179478858 1104 28-29 0.12629165280029042 1104 30-31 0.04158758484003755 1104 32-33 0.1816911041231677 1104 34-35 0.04383593379664319 1104 36-37 0.17391300209606442 1104 38-39 0.35997584236624647 1104 40-41 0.45887489018213756 1104 42-43 0.3253801534216194 1104 44-45 0.22481488043714393 1104 46-47 0.5388844150192895 1104 48-49 0.4720253788944646 1104 50-51 0.3560291808763125 1104 52-53 0.3098715800058258 1104 54-55 0.3888074402618571 1104 56-57 0.4277472220040721 1104 58-59 0.4606410298519492 1104 60-61 0.5766998101626868 1104 62-63 0.32935136167849066 1104 64-65 0.38263319166582477 1104 66-67 0.3690827439677662 1104 68-69 0.3255335987854977 1104 70-71 0.2122214422236084 1104 72-73 0.3065663535306129 1104 74-75 0.3716166050598986 1104 76-77 0.03054089342399635 1104 78-79 0.35604836755054237 1104 80-81 0.500710726463744 1104 82-83 0.6632002321252557 1104 84-85 0.5208339618772904 1104 86-87 0.4829186100762257 1104 88-89 0.39075733194898987 1104 90-91 0.6099703258038431 1104 92-93 0.6690552482594754 1104 94-95 0.8081047299847413 1104 96-97 0.6675246508130925 1104 98-99 0.5141053820989505 1104 100-101 0.7439667888189874 1105 1 -0.05472978078577029 1105 2 0.1545871564339052 1105 3 0.07364379390982378 1105 4 0.058935416421697084 1105 5 0.008930811163416763 1105 6 0.11657138733038863 1105 7 0.18721659825823167 1105 8 0.1798540217640081 1105 9 0.40449578986144274 1105 10-11 0.14721772115067466 1105 12-13 0.21732064475010304 1105 14-15 0.14994253401558666 1105 16-17 0.3517004797054497 1105 18-19 0.3136730348926804 1105 20-21 0.03254644458037603 1105 22-23 0.05211670173821403 1105 24-25 0.1179524338145086 1105 26-27 0.05911091271085667 1105 28-29 0.03679789630753305 1105 30-31 3.348860599245995E-4 1105 32-33 -0.11013504911873895 1105 34-35 0.059275628697953664 1105 36-37 0.11068775940920972 1105 38-39 0.16016850447316955 1105 40-41 0.10461366453507281 1105 42-43 -0.05604317565873629 1105 44-45 -0.2379890155671518 1105 46-47 -0.19102841060639975 1105 48-49 -0.1631018733813434 1105 50-51 -0.26214659482688063 1105 52-53 -0.2557601959948457 1105 54-55 -0.23062447378621442 1105 56-57 -0.3733704041384769 1105 58-59 -0.08112179356590588 1105 60-61 -0.1397824447044158 1105 62-63 -0.039468751408819 1105 64-65 -0.0597273723298386 1105 66-67 -0.012526507531809727 1105 68-69 -0.08065227250506268 1105 70-71 -0.0752378581968074 1105 72-73 -0.019493927165761704 1105 74-75 0.0726417347988857 1105 76-77 -0.10108520729482251 1105 78-79 -0.15911011969168953 1105 80-81 0.004280436912814878 1105 82-83 0.04997812687922476 1105 84-85 -0.045734398310212754 1105 86-87 -0.11249096539317094 1105 88-89 0.09865461910848694 1105 90-91 0.02485620429889579 1105 92-93 0.16536004017742556 1105 94-95 -0.28287640586179563 1105 96-97 -0.29984015453775115 1105 98-99 -0.3066723778612648 1105 100-101 -0.06653178538846305 1106 1 -0.011746658283541933 1106 2 -0.03318574048454792 1106 3 -0.065905478915532 1106 4 -0.02371037631426276 1106 5 -0.09382045217179069 1106 6 -0.038805081055954815 1106 7 -0.005092194995178545 1106 8 -0.044280910142504126 1106 9 -0.011516364579222227 1106 10-11 0.08412912804919159 1106 12-13 0.015286097073648364 1106 14-15 0.03097654222864321 1106 16-17 0.09801506393329618 1106 18-19 0.06019789975159284 1106 20-21 -0.08416040660479496 1106 22-23 -0.00558276210122699 1106 24-25 0.027238910898326196 1106 26-27 0.05444730667327491 1106 28-29 0.10094890202144313 1106 30-31 0.1045052684183716 1106 32-33 0.05923994400035326 1106 34-35 0.14086561449575896 1106 36-37 0.012384316751962388 1106 38-39 0.2843760987966917 1106 40-41 0.1391140003235165 1106 42-43 0.3441693068637477 1106 44-45 0.3354297511534625 1106 46-47 0.02245487153296466 1106 48-49 0.10516226551396812 1106 50-51 0.20142861627946473 1106 52-53 0.21044517441833932 1106 54-55 0.28008992988655024 1106 56-57 0.25182694038046094 1106 58-59 0.2883996253329215 1106 60-61 0.09536524716289563 1106 62-63 0.1261189884597229 1106 64-65 0.26790306221344196 1106 66-67 0.22995348277986238 1106 68-69 0.35599821025962797 1106 70-71 0.41794838878935536 1106 72-73 0.15918295627711387 1106 74-75 0.42866225642696776 1106 76-77 0.27009753868397723 1106 78-79 0.25106371963629215 1106 80-81 0.026174944294659497 1106 82-83 0.17932273389664743 1106 84-85 0.13638624332094906 1106 86-87 0.17309215050338622 1106 88-89 0.24134048626837767 1106 90-91 0.4370293610405369 1106 92-93 0.5857187282905194 1106 94-95 0.6121153920926439 1106 96-97 0.5788840695707549 1106 98-99 0.47757529088618966 1106 100-101 0.8939167398947561 1107 1 -0.008756383292912062 1107 2 0.10134796626562093 1107 3 0.03701863954875506 1107 4 0.0018503002464740348 1107 5 0.025779521365599578 1107 6 0.08082797074460757 1107 7 -0.002584862646841657 1107 8 -0.05981005209235235 1107 9 0.016756729561514305 1107 10-11 -0.009867167185326764 1107 12-13 0.02632872392879193 1107 14-15 0.03400540852629774 1107 16-17 0.16200496403694586 1107 18-19 0.15284019141366656 1107 20-21 -0.0026125644101639978 1107 22-23 -0.04064461845335643 1107 24-25 -0.07120310290634535 1107 26-27 0.0841174061285841 1107 28-29 0.17793745694238794 1107 30-31 -0.05142676677690616 1107 32-33 -0.03175835798214166 1107 34-35 -0.04587330979912707 1107 36-37 -0.16153567795551282 1107 38-39 -0.12131887551252163 1107 40-41 -0.08195134027233308 1107 42-43 -0.14850716567026723 1107 44-45 -0.1542751907390567 1107 46-47 -0.1476842776941112 1107 48-49 -0.1518591471483859 1107 50-51 -0.17203048796117315 1107 52-53 -0.04804601573756884 1107 54-55 0.009396168446976105 1107 56-57 -0.1315247697657398 1107 58-59 0.0151345802362286 1107 60-61 0.2573902984342098 1107 62-63 0.3295282547838525 1107 64-65 0.3465190676823724 1107 66-67 0.06172515455528327 1107 68-69 0.2761792586200542 1107 70-71 0.41694611741528576 1107 72-73 0.3905312262839544 1107 74-75 0.38015518870474097 1107 76-77 0.12036767783319391 1107 78-79 0.26766349470410944 1107 80-81 0.4543973184318659 1107 82-83 0.5766808832780939 1107 84-85 0.26875663165975894 1107 86-87 0.37050653293221103 1107 88-89 0.4648150270318254 1107 90-91 0.06723202053289512 1107 92-93 0.5036636860606016 1107 94-95 0.50769834135059 1107 96-97 0.28489438998886385 1107 98-99 0.4535439326927069 1107 100-101 0.7643932874061683 1108 1 -0.10243336864932928 1108 2 -0.0897375074041733 1108 3 -0.0813538774184579 1108 4 -0.013323907878955765 1108 5 -0.02593517271106549 1108 6 -0.021570118999754584 1108 7 0.03550043129460079 1108 8 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-1.2956083612127323 2316 100-101 -1.3578118319737982 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 6.0 5 18.0 6 43.0 7 118.0 8 202.0 9 337.0 10 565.0 11 958.0 12 1669.0 13 2581.0 14 3008.0 15 3931.0 16 5076.0 17 7505.0 18 6885.0 19 3484.0 20 2609.0 21 2798.0 22 2863.0 23 3265.0 24 3679.0 25 4307.0 26 5026.0 27 5923.0 28 7421.0 29 9003.0 30 11192.0 31 13825.0 32 17226.0 33 22138.0 34 30168.0 35 51628.0 36 68063.0 37 136745.0 38 239047.0 39 57943.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.55272760182485 22.32447100993189 14.442584128995279 20.680217259247986 2 13.65972084498759 24.43611644908259 40.50277992436418 21.401382781565648 3 18.01303475021743 30.794118773212848 31.05175063860977 20.14109583795995 4 9.84840197134871 19.117132245554348 42.78931620892796 28.245149574168988 5 9.082617233438537 42.926883967202535 36.65114129275403 11.339357506604895 6 24.788998955250822 42.66227799079964 19.06421105027377 13.484512003675766 7 24.379167373194253 36.38585157997801 24.134799993436133 15.100181053391607 8 23.720592498591504 35.69350012854244 22.420946400538238 18.16496097232782 9 26.073739600369766 17.44854200055793 23.64907367396168 32.82864472511063 10-11 21.94230757396846 28.98015724601364 29.950791392288394 19.126743787729506 12-13 24.037315621814216 28.390599712420283 29.312755589377737 18.259329076387765 14-15 21.50262007778185 29.817538111465435 28.956577270415 19.723264540337713 16-17 17.19104903367142 33.11259953533086 30.361233082583382 19.33511834841434 18-19 16.645356883037323 32.79343230189422 33.41993994059698 17.141270874471473 20-21 18.18861825083826 31.319843670515645 32.599661413748024 17.891876664898067 22-23 17.907353594748898 30.822057365560152 32.50090595193327 18.769683087757684 24-25 18.1795493659219 31.267739110041116 32.92242073083595 17.630290793201038 26-27 17.638459644996306 30.99089243224024 33.84815250389738 17.522495418866065 28-29 17.192241368700454 30.749128562500772 34.40499519324784 17.65363487555093 30-31 17.36533701642348 31.74614027650526 33.53398881398116 17.354533893090103 32-33 16.838514523423047 32.35336751145614 33.3426094709593 17.4655084941615 34-35 17.473508308182918 30.90745240794402 33.722566449737236 17.89647283413582 36-37 17.871774815527928 30.493001274484598 33.18343552912991 18.451788380857572 38-39 17.304820614925145 31.30042555751864 34.00740623239379 17.387347595162428 40-41 16.688888250730578 31.357800322999267 34.67025220472301 17.28305922154714 42-43 16.669207958093992 31.568514698350985 32.79954817433852 18.962729169216505 44-45 17.073641025641027 31.796444444444443 32.60382905982906 18.52608547008547 46-47 17.48461055577058 31.227203039457972 32.72128865140464 18.566897753366803 48-49 17.54733160915846 31.523405605528666 32.487270419641916 18.44199236567096 50-51 17.70484606800578 31.328155769988186 32.38760065205024 18.5793975099558 52-53 17.079386482847653 32.0314567374131 32.50716433280249 18.381992446936756 54-55 17.5006341676541 31.208227838148105 32.637515238826516 18.65362275537128 56-57 18.2934692457014 31.930221379531503 30.936109198494915 18.84020017627218 58-59 17.787508239135963 31.1159687446155 32.10370781630761 18.992815199940924 60-61 18.043614712546855 32.27760858892853 31.481114285206363 18.197662413318255 62-63 18.08106651934427 32.19953888818996 32.15619000213326 17.563204590332514 64-65 17.832376779607014 31.398072670733555 32.274560320319004 18.49499022934042 66-67 17.764017963121994 31.87079843998709 31.23335101931419 19.13183257757673 68-69 17.298803734841563 31.967615510422874 31.895139454870662 18.838441299864893 70-71 18.323942207827283 31.02633685936422 32.177476204248606 18.472244728559893 72-73 18.02409101444955 30.994019296605828 31.444332690386634 19.537556998557985 74-75 17.57852028509072 32.232289860463084 31.102142009309752 19.087047845136446 76-77 17.879114645562268 30.867514715715398 32.16014096394787 19.093229674774467 78-79 17.036197933600047 31.064240466257587 32.44836566860256 19.451195931539807 80-81 17.93995377904194 32.20554651496711 30.694750229053565 19.15974947693738 82-83 18.358048124100886 30.98009506670532 31.376935657672345 19.284921151521452 84-85 17.251241672045424 31.013015676793316 31.325074664420356 20.41066798674091 86-87 17.856834801276584 31.759669873642327 31.094132288772936 19.28936303630815 88-89 18.28020369646915 30.753723705713078 31.335595710379057 19.630476887438718 90-91 17.790192043895747 30.011316872427983 32.26646090534979 19.932030178326475 92-93 18.263245349889008 31.89623307234786 31.126169342699278 18.714352235063856 94-95 17.717730653571444 30.678186524883795 31.44733945154863 20.15674336999613 96-97 17.707237190729668 30.684198110788607 31.16774870685087 20.440815991630856 98-99 17.693823520563193 31.19035962683485 30.784757226587605 20.331059626014348 100-101 17.472342636782585 30.68538296388475 31.3085454073051 20.53372899202757 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2532.0 1 1537.0 2 1063.5 3 1606.0 4 1152.0 5 1203.0 6 2898.0 7 4754.5 8 5580.5 9 5023.5 10 3530.5 11 2536.5 12 2313.0 13 2211.5 14 2051.0 15 1876.5 16 1764.0 17 1711.5 18 1662.0 19 1694.0 20 1866.5 21 2124.0 22 2370.0 23 2724.0 24 2966.5 25 3457.0 26 5266.5 27 7321.0 28 7751.0 29 9094.0 30 13238.5 31 16696.0 32 21677.0 33 28588.5 34 32582.0 35 36691.5 36 44345.0 37 47785.5 38 42959.0 39 38332.5 40 34771.0 41 30640.5 42 25251.0 43 24166.0 44 25494.5 45 23311.5 46 19389.0 47 19215.0 48 32383.5 49 30671.0 50 16498.5 51 10505.5 52 5685.0 53 3885.5 54 2952.5 55 2430.0 56 2047.0 57 1740.5 58 1409.0 59 1166.0 60 877.5 61 590.0 62 367.0 63 210.0 64 109.0 65 63.0 66 39.5 67 25.5 68 20.0 69 19.0 70 18.5 71 13.5 72 11.0 73 10.0 74 8.0 75 4.0 76 3.0 77 3.5 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27472527472527475 2 0.019281365722381153 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.05120911940225E-4 12-13 6.837363731340835E-5 14-15 0.0 16-17 4.1024182388045E-4 18-19 0.0 20-21 0.0 22-23 1.367472746268167E-4 24-25 0.002393077305969292 26-27 0.002187956394029067 28-29 0.001777714570148617 30-31 8.204836477609E-4 32-33 0.0012307254716413501 34-35 0.0012307254716413501 36-37 0.0 38-39 0.0 40-41 6.837363731340834E-4 42-43 0.0028033191298497424 44-45 0.003555429140297234 46-47 0.012717496540293954 48-49 0.014358463835815752 50-51 0.006016880083579934 52-53 0.0067689900940274255 54-55 3.418681865670417E-4 56-57 0.0028033191298497424 58-59 0.0013674727462681668 60-61 0.0012307254716413501 62-63 0.0 64-65 6.837363731340834E-4 66-67 0.0 68-69 0.0 70-71 6.837363731340835E-5 72-73 0.0010256045597011251 74-75 0.0 76-77 0.1974630645611233 78-79 0.10645775329697679 80-81 8.204836477609E-4 82-83 0.0 84-85 5.469890985072668E-4 86-87 0.12156832714324003 88-89 0.5896542481908336 90-91 0.3112367970506348 92-93 0.29817743232377375 94-95 0.0023247036686558837 96-97 0.0025298245805961085 98-99 0.0024614509432827002 100-101 8.204836477609E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 731276.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.241191676475275 #Duplication Level Percentage of deduplicated Percentage of total 1 68.34958262048228 13.151274202077829 2 12.27122762822538 4.722260858006872 3 5.240953750836844 3.0252658706218125 4 2.908570786288435 2.2385747201428874 5 1.8373535144845754 1.7676435574821605 6 1.2971082900944457 1.4974745540911394 7 0.9452950693041899 1.273202253351623 8 0.7413869392078783 1.1412134562987288 9 0.5721821260556845 0.9908519365161508 >10 4.377091384173449 17.2333313522509 >50 0.6898778939217897 9.388402079378551 >100 0.6870388902430992 25.945622852734985 >500 0.05607032265413723 7.3740751899021575 >1k 0.02484128218854181 8.380965505570593 >5k 0.0014195018393452464 1.869841611573612 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7935 1.0850896241637904 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5757 0.7872540600265836 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4475 0.6119440539550046 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4447 0.6081151302654538 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3520 0.4813504066863947 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3000 0.41024182388045005 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2953 0.40381470197298974 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2607 0.35650014495211113 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2030 0.27759696749243784 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1987 0.27171683468348473 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1872 0.25599089810140085 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1808 0.24723907252528457 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1805 0.24682883070140413 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1796 0.2455981052297628 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1759 0.24053845606857052 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1568 0.21441972661484857 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1549 0.21182152839693907 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1513 0.20689862651037363 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 1490 0.20375343919395686 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1368 0.18707027168948523 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1337 0.18283110617605391 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 1279 0.17489976424769854 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1277 0.1746262696984449 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1247 0.1705238514596404 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1228 0.1679256532417309 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1227 0.16778890596710408 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1216 0.1662846859462091 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1210 0.1654642022984482 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 1207 0.16505396047456775 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 1142 0.15616538762382465 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 1103 0.1508322439133788 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 1100 0.15042200208949835 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1075 0.14700332022382795 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 1063 0.14536235292830615 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1049 0.1434478910835307 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1036 0.1416701765133821 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1027 0.14043945104174074 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 992 0.13565329642980214 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 976 0.13346534003577307 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 971 0.132781603662639 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 945 0.12922617452234178 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 942 0.1288159326984613 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 941 0.1286791854238345 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 934 0.12772195450144677 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 924 0.1263544817551786 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 896 0.12252555806562776 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 885 0.12102133804473277 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 851 0.116371930707421 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 851 0.116371930707421 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 842 0.11514120523577966 No Hit CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA 834 0.1140472270387651 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 833 0.1139104797641383 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 828 0.11322674339100422 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 823 0.11254300701787014 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 816 0.11158577609548241 No Hit GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT 811 0.11090203972234833 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 806 0.11021830334921424 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 791 0.10816709422981201 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 770 0.10529540146264885 No Hit GGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATAC 759 0.10379118144175387 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 759 0.10379118144175387 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 758 0.10365443416712704 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 758 0.10365443416712704 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 743 0.10160322504772479 No Hit GTACATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT 735 0.10050924685071026 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.0512091194022504E-4 0.0 12-13 0.0 0.0 0.0 5.469890985072668E-4 0.0 14-15 0.0 0.0 0.0 8.204836477609001E-4 0.0 16-17 0.0 0.0 0.0 0.0026665718552229254 0.0 18-19 0.0 0.0 0.0 0.00458103369999836 0.0 20-21 0.0 0.0 0.0 0.010597913783578293 0.0 22-23 0.0 0.0 0.0 0.028375059485064462 0.0 24-25 0.0 0.0 0.0 0.04485310607759588 0.0 26-27 0.0 0.0 0.0 0.052100711632817154 0.0 28-29 0.0 0.0 0.0 0.0598269326492323 0.0 30-31 0.0 0.0 0.0 0.0778091992626587 0.0 32-33 0.0 0.0 0.0 0.1056372696492159 0.0 34-35 0.0 0.0 0.0 0.1450888583790525 0.0 36-37 0.0 0.0 0.0 0.208129351982015 0.0 38-39 0.0 0.0 0.0 0.2901093431207916 0.0 40-41 0.0 0.0 0.0 0.3878152708416521 0.0 42-43 0.0 0.0 0.0 0.47861546119385845 0.0 44-45 0.0 0.0 0.0 0.5696207724580049 0.0 46-47 0.0 0.0 0.0 0.6928984405340801 0.0 48-49 0.0 0.0 0.0 0.8271158905803007 0.0 50-51 0.0 0.0 0.0 0.9579146587608509 0.0 52-53 0.0 0.0 0.0 1.0792778649921506 0.0 54-55 0.0 0.0 0.0 1.2037178849025538 0.0 56-57 0.0 0.0 0.0 1.3490118641935465 0.0 58-59 0.0 0.0 0.0 1.5108522637143842 0.0 60-61 0.0 0.0 0.0 1.7074948446277465 0.0 62-63 0.0 0.0 0.0 1.8854030489172349 0.0 64-65 0.0 0.0 0.0 2.0512091194022504 0.0 66-67 0.0 0.0 0.0 2.2494242939738207 0.0 68-69 0.0 0.0 0.0 2.4812109244662754 0.0 70-71 0.0 0.0 0.0 2.74349219720051 0.0 72-73 0.0 0.0 0.0 2.981158960501917 0.0 74-75 0.0 0.0 0.0 3.245354695080927 0.0 76-77 0.0 0.0 0.0 3.517481771588292 0.0 78-79 0.0 0.0 0.0 3.8462905934284732 0.0 80-81 0.0 0.0 0.0 4.199987419250734 0.0 82-83 0.0 0.0 0.0 4.545274287683446 0.0 84-85 0.0 0.0 0.0 4.916611511932567 0.0 86-87 0.0 0.0 0.0 5.3083924537383975 0.0 88-89 0.0 0.0 0.0 5.726018630448696 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCATGCG 20 0.0020825714 71.173996 9 GTATGGT 75 6.8398895E-6 38.063576 1 TACAGGG 100 3.0568117E-8 37.959465 2 CATGGGT 400 0.0 36.77323 4 GGGTATG 315 0.0 34.645542 7 GATGGTC 55 0.0025925476 34.508602 8 TGGGTAT 395 0.0 32.43372 6 AGGCTTA 740 0.0 32.060356 6 GGCATTC 150 9.640644E-10 31.632887 7 CTCCCGC 105 1.916318E-6 31.632885 7 ATGGGTA 445 0.0 30.922035 5 GGATGAC 320 0.0 29.655832 8 CATTCCG 160 1.9244908E-9 29.655832 9 CGTCATG 40 4.5224102E-4 29.655832 48-49 ATATAGG 815 0.0 29.110018 2 GGGATGC 310 0.0 29.081848 7 ACTCCCG 115 3.894267E-6 28.8822 6 CTTACTA 830 0.0 28.583933 9 GGTATGC 185 2.928573E-10 28.213116 8 GTACAGG 120 5.308677E-6 27.75469 1 >>END_MODULE