FastQCFastQC Report
Thu 26 May 2016
SRR936361_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936361_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2755165
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31860.1156373574722385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26300.043.031441
GTATCAA45200.041.0668451
TCAACGC57350.031.8578344
ATCAACG58550.031.045473
CAACGCA60100.030.24265
AACGCAG61100.029.9773146
TATCAAC61800.029.7202842
GTACATG94950.029.473811
TACATGG95700.028.392892
ACATGGG97600.026.7721463
ACGCAGA75550.024.0562327
CATGGGG77150.023.5590444
CGCAGAG79750.022.7307998
GAGTACA69250.022.701891
TACCTGG25400.022.1780262
GTACCTG26700.021.8141141
ATCGCGT1100.002938483821.5141358
GTGGTAT17150.021.2603451
AGTACAT67450.020.9845142
GTGTAGG12750.019.683821
Produced by FastQC (version 0.11.5)