##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936361_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2755165 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.592281768968466 33.0 31.0 34.0 30.0 34.0 2 31.68428351840997 33.0 31.0 34.0 30.0 34.0 3 31.752039170067853 34.0 31.0 34.0 30.0 34.0 4 35.24343768884985 37.0 35.0 37.0 33.0 37.0 5 35.21247584082986 37.0 35.0 37.0 33.0 37.0 6 35.30097217408032 37.0 35.0 37.0 33.0 37.0 7 35.232488798311536 37.0 35.0 37.0 33.0 37.0 8 35.23109287465542 37.0 35.0 37.0 33.0 37.0 9 36.87914444325476 39.0 37.0 39.0 33.0 39.0 10-11 36.79115189108456 39.0 37.0 39.0 33.0 39.0 12-13 36.681763342667324 39.0 37.0 39.0 32.0 39.0 14-15 37.91998174338016 40.0 38.0 41.0 33.0 41.0 16-17 37.85622349296685 40.0 38.0 41.0 33.0 41.0 18-19 37.766966588207964 40.0 38.0 41.0 32.0 41.0 20-21 37.66718653873724 40.0 38.0 41.0 32.0 41.0 22-23 37.49835436353177 40.0 37.5 41.0 32.0 41.0 24-25 37.65274929087732 40.0 38.0 41.0 32.0 41.0 26-27 37.596509646427705 40.0 38.0 41.0 32.0 41.0 28-29 37.45820867352772 40.0 37.5 41.0 32.0 41.0 30-31 37.308896381886385 40.0 37.0 41.0 31.0 41.0 32-33 37.13828645471324 40.0 37.0 41.0 31.0 41.0 34-35 36.875086610057835 40.0 37.0 41.0 30.5 41.0 36-37 36.673725711527254 40.0 36.0 41.0 30.0 41.0 38-39 36.50881961697394 40.0 36.0 41.0 30.0 41.0 40-41 36.308829416749994 40.0 36.0 41.0 30.0 41.0 42-43 36.106654955329354 39.0 35.0 41.0 29.0 41.0 44-45 35.92455660550276 39.0 35.0 41.0 28.0 41.0 46-47 35.65567488698499 39.0 35.0 41.0 27.5 41.0 48-49 35.49182009062978 39.0 35.0 41.0 27.0 41.0 50-51 35.17677761585966 38.5 34.5 40.5 26.5 41.0 52-53 35.172636666043594 39.0 35.0 40.0 27.0 41.0 54-55 35.2098104832197 39.0 35.0 41.0 27.0 41.0 56-57 35.00039017626894 38.0 35.0 40.5 26.0 41.0 58-59 34.719570697217776 38.0 34.0 40.0 26.0 41.0 60-61 34.370951830471135 37.0 34.0 40.0 26.0 41.0 62-63 34.013759430015995 37.0 34.0 40.0 25.5 41.0 64-65 33.64040756179757 36.0 33.5 39.0 25.0 41.0 66-67 33.25182012692525 35.5 33.0 39.0 23.5 41.0 68-69 32.84819348387483 35.0 33.0 38.5 23.0 40.0 70-71 32.43585338809109 35.0 33.0 37.5 22.0 40.0 72-73 32.04215065159437 35.0 32.0 37.0 20.5 39.0 74-75 31.640159845236127 35.0 32.0 36.0 20.0 39.0 76-77 31.270436616318804 35.0 32.0 36.0 18.5 38.5 78-79 30.912603419395936 35.0 31.0 35.5 17.5 37.0 80-81 30.562115517582434 35.0 31.0 35.0 14.0 37.0 82-83 30.25563387310742 34.0 31.0 35.0 9.0 36.0 84-85 29.978342676391435 34.0 31.0 35.0 7.0 36.0 86-87 29.682869991452417 34.0 30.0 35.0 3.5 36.0 88-89 29.378858072021096 34.0 30.0 35.0 2.0 35.0 90-91 29.044384819058024 34.0 29.5 35.0 2.0 35.0 92-93 28.733161716267446 34.0 29.0 35.0 2.0 35.0 94-95 28.353408053601143 34.0 29.0 35.0 2.0 35.0 96-97 27.908524716305557 34.0 29.0 35.0 2.0 35.0 98-99 27.370522999529975 34.0 28.0 35.0 2.0 35.0 100-101 24.074433836086044 29.5 14.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14092153517340478 1101 2 -6.064149089297644E-4 1101 3 0.1623720430984541 1101 4 0.005603609917343988 1101 5 0.2973004358393041 1101 6 0.10600352700207338 1101 7 0.24450695566549996 1101 8 0.15755698445364885 1101 9 0.29223586297112547 1101 10-11 0.41328162112052524 1101 12-13 0.22942217955011301 1101 14-15 0.2757986044253258 1101 16-17 0.3438387802302785 1101 18-19 0.4000580581242872 1101 20-21 0.4778320940182539 1101 22-23 0.4139020272514742 1101 24-25 0.3785927778268885 1101 26-27 0.25267354839703415 1101 28-29 0.3141290942314896 1101 30-31 0.3415315455993664 1101 32-33 0.4180160234551593 1101 34-35 0.37486490957793706 1101 36-37 0.1735900928806089 1101 38-39 0.22684428017328884 1101 40-41 0.12322125477904677 1101 42-43 0.2174990829983372 1101 44-45 0.2745363694607619 1101 46-47 0.004312205654855461 1101 48-49 0.11376510005194973 1101 50-51 0.14771888521543985 1101 52-53 0.21611644911004646 1101 54-55 0.10704811116067958 1101 56-57 0.1964202131677979 1101 58-59 0.2284530470150088 1101 60-61 0.0798285636177809 1101 62-63 0.10126608483765409 1101 64-65 0.026061659441914742 1101 66-67 0.10347968741957914 1101 68-69 0.09485848515327433 1101 70-71 0.06415712501706139 1101 72-73 0.06150790653170901 1101 74-75 -0.04838917400942222 1101 76-77 -0.06318354263770587 1101 78-79 -0.04950829144744162 1101 80-81 -0.07288893418263243 1101 82-83 0.19520987567059933 1101 84-85 0.16396093474592632 1101 86-87 0.10279540296276934 1101 88-89 -0.17316063570848783 1101 90-91 0.06643555312581029 1101 92-93 0.07117208478924297 1101 94-95 0.32021837444109025 1101 96-97 0.2656896603304446 1101 98-99 0.34639439326577914 1101 100-101 0.36973765347450893 1102 1 0.284767406624848 1102 2 0.2712587513905049 1102 3 0.14207851404632876 1102 4 0.1795006385472604 1102 5 0.27140878264398083 1102 6 0.22858386195243696 1102 7 0.2567489148001272 1102 8 0.14531678016186333 1102 9 0.287940709636473 1102 10-11 0.3317897906158791 1102 12-13 0.41833996095144244 1102 14-15 0.4616122443273696 1102 16-17 0.38891621793510467 1102 18-19 0.3176440577073336 1102 20-21 0.30441852459070873 1102 22-23 0.4089536336697108 1102 24-25 0.4031387297905198 1102 26-27 0.4792286725732211 1102 28-29 0.33781605062505093 1102 30-31 0.36821147419832556 1102 32-33 0.10706410193734683 1102 34-35 0.17603340677966628 1102 36-37 0.3293474865599464 1102 38-39 0.2921232054233016 1102 40-41 0.2603855621180102 1102 42-43 0.2378113888688489 1102 44-45 0.21566814833091286 1102 46-47 0.2893422029383572 1102 48-49 0.3574047599212946 1102 50-51 0.19742954672801716 1102 52-53 0.30795606138804743 1102 54-55 0.30308335963589883 1102 56-57 0.36150538621986783 1102 58-59 0.3048674152470241 1102 60-61 0.2907791865518874 1102 62-63 0.41823825794377 1102 64-65 0.3641013350346469 1102 66-67 0.53801729335067 1102 68-69 0.6299660464902956 1102 70-71 0.5390389188033708 1102 72-73 0.438583797869029 1102 74-75 0.48411525345906625 1102 76-77 0.3770177308166467 1102 78-79 0.44976213264191856 1102 80-81 0.41270143706490003 1102 82-83 0.5217589280905663 1102 84-85 0.4641242579690221 1102 86-87 0.2742189806100015 1102 88-89 0.29866852121587684 1102 90-91 0.30824956224678246 1102 92-93 0.5597001506927164 1102 94-95 0.4515452848156478 1102 96-97 0.4884926672887495 1102 98-99 0.15641826614777088 1102 100-101 0.42249007182342524 1103 1 0.41593639199481913 1103 2 0.3237503623664644 1103 3 0.4221474477396434 1103 4 0.2817236118575437 1103 5 0.2815565670916058 1103 6 0.3060489262379278 1103 7 0.3131348274463335 1103 8 0.35300331198348545 1103 9 0.3895163754625486 1103 10-11 0.43953815227693127 1103 12-13 0.49262616868081466 1103 14-15 0.48719320195115756 1103 16-17 0.45988948639602256 1103 18-19 0.43705913325914736 1103 20-21 0.5030893011033228 1103 22-23 0.5770884638720872 1103 24-25 0.6007076668569837 1103 26-27 0.5628585057911977 1103 28-29 0.5530454518099646 1103 30-31 0.6162607815557024 1103 32-33 0.5802936029675081 1103 34-35 0.4374655180882172 1103 36-37 0.4475483967707419 1103 38-39 0.5007734461987496 1103 40-41 0.4398172403141061 1103 42-43 0.4257009033646213 1103 44-45 0.4001971621432858 1103 46-47 0.5846881329630378 1103 48-49 0.5005985190541011 1103 50-51 0.5642984000054341 1103 52-53 0.5726938596925706 1103 54-55 0.5647102703567057 1103 56-57 0.6240071233921185 1103 58-59 0.7130777635315084 1103 60-61 0.5787536032330678 1103 62-63 0.5628225981623771 1103 64-65 0.5856881120168111 1103 66-67 0.6363177991882409 1103 68-69 0.6637349884140988 1103 70-71 0.67314608647726 1103 72-73 0.6359198189282793 1103 74-75 0.6333606123419884 1103 76-77 0.5694835979118373 1103 78-79 0.5856810191785975 1103 80-81 0.6982977829989743 1103 82-83 0.7786104703622065 1103 84-85 0.8785374917809392 1103 86-87 0.9841037942839428 1103 88-89 0.8746048739232357 1103 90-91 0.8771974227926655 1103 92-93 0.9560260482541985 1103 94-95 0.9364323922315876 1103 96-97 1.0181478841350682 1103 98-99 1.1211874010157494 1103 100-101 1.210608184694422 1104 1 0.27190311983907023 1104 2 0.22722639718818982 1104 3 0.24948412439139034 1104 4 0.22479645541387328 1104 5 0.2871843104308667 1104 6 0.25670528180967267 1104 7 0.25023048291426875 1104 8 0.27646699249793727 1104 9 0.3453995533441159 1104 10-11 0.35649800275813703 1104 12-13 0.30187206757216245 1104 14-15 0.5588066071664954 1104 16-17 0.602197643208612 1104 18-19 0.42389555945899104 1104 20-21 0.5252749179706484 1104 22-23 0.6010396181616784 1104 24-25 0.6483332904294272 1104 26-27 0.5532213871693941 1104 28-29 0.5080667319722636 1104 30-31 0.4864273733177811 1104 32-33 0.7269797184288009 1104 34-35 0.7201087392890528 1104 36-37 0.6173041352210831 1104 38-39 0.6558647143037177 1104 40-41 0.7619769210555702 1104 42-43 0.8649730815570322 1104 44-45 0.7786781631061572 1104 46-47 0.7166093003394849 1104 48-49 0.6849825954607383 1104 50-51 0.7090924321886689 1104 52-53 0.6582911594316911 1104 54-55 0.8215079441567994 1104 56-57 0.9317994773166234 1104 58-59 0.901991917594728 1104 60-61 0.7830840783129389 1104 62-63 0.7958728779491651 1104 64-65 0.6906871847838119 1104 66-67 0.8145710836059052 1104 68-69 0.7451681540535446 1104 70-71 0.6518639443248588 1104 72-73 0.8075059351155218 1104 74-75 0.7831450389193151 1104 76-77 0.7275152835422354 1104 78-79 0.5956223758161663 1104 80-81 0.7893279751572884 1104 82-83 0.829215448646476 1104 84-85 0.45545740854775474 1104 86-87 0.5376036533841244 1104 88-89 0.6227037163008831 1104 90-91 0.7264035991543594 1104 92-93 0.7420330463885172 1104 94-95 0.5432654724732089 1104 96-97 0.82657181242201 1104 98-99 0.9912627543707195 1104 100-101 1.1066318730227032 1105 1 0.20156774623407614 1105 2 0.2405611790576181 1105 3 0.25505755986794654 1105 4 0.12088338300774382 1105 5 0.15464116853679855 1105 6 0.07852987801684463 1105 7 0.21637218381492573 1105 8 0.21037662248803457 1105 9 0.110174862574965 1105 10-11 0.2386818766300678 1105 12-13 0.2648453636164021 1105 14-15 0.2641516250005296 1105 16-17 0.32724663576354374 1105 18-19 0.35208526647435434 1105 20-21 0.3716493885505301 1105 22-23 0.43938941841476975 1105 24-25 0.34942068890467226 1105 26-27 0.3412065677451679 1105 28-29 0.26314300993305295 1105 30-31 0.377818183320521 1105 32-33 0.49011979969039743 1105 34-35 0.15511939599339541 1105 36-37 0.24047014870725292 1105 38-39 0.37725304615262445 1105 40-41 0.2827971096202546 1105 42-43 0.3552758984561777 1105 44-45 0.33084474775697004 1105 46-47 0.41758444262440264 1105 48-49 0.4446220835403665 1105 50-51 0.45033869928865045 1105 52-53 0.44023387132911296 1105 54-55 0.6518080836212548 1105 56-57 0.7443565495851843 1105 58-59 0.6584808396685204 1105 60-61 0.6111093625533215 1105 62-63 0.4190053029689338 1105 64-65 0.508299803376012 1105 66-67 0.5117177032080136 1105 68-69 0.5649377468166961 1105 70-71 0.5576350374799688 1105 72-73 0.4956574348485887 1105 74-75 0.5910762240264802 1105 76-77 0.5465752596863886 1105 78-79 0.4163186245469568 1105 80-81 0.4106123113286593 1105 82-83 0.3456886628891418 1105 84-85 0.3717763247684758 1105 86-87 0.48339210968713786 1105 88-89 0.5706637714181078 1105 90-91 0.5407637613689751 1105 92-93 0.3294704938964905 1105 94-95 0.4475550571236937 1105 96-97 0.5380973491383827 1105 98-99 0.5273178034564872 1105 100-101 0.5297944794491265 1106 1 0.2556128485191067 1106 2 0.21922093175007973 1106 3 0.23556214702390932 1106 4 0.1840208837200379 1106 5 0.18277667483344828 1106 6 0.18635653480640713 1106 7 0.2209450939522526 1106 8 0.2145613338676 1106 9 0.32939675556031744 1106 10-11 0.2507943901635983 1106 12-13 0.2911556234602699 1106 14-15 0.33981120674307164 1106 16-17 0.348650989287961 1106 18-19 0.3034891926239922 1106 20-21 0.23137608237923502 1106 22-23 0.37758212170270156 1106 24-25 0.3283154977256828 1106 26-27 0.2784234319539465 1106 28-29 0.2763239604145582 1106 30-31 0.3965496637467609 1106 32-33 0.2867089928068154 1106 34-35 0.2660053439106491 1106 36-37 0.3933919676143134 1106 38-39 0.3578822842858429 1106 40-41 0.23347449864527192 1106 42-43 0.304006951507624 1106 44-45 0.4536544520136232 1106 46-47 0.2758313512801678 1106 48-49 0.2929503358624359 1106 50-51 0.2855305193353814 1106 52-53 0.23385102900191157 1106 54-55 0.2620494079132456 1106 56-57 0.2900304483828293 1106 58-59 0.2729727622851996 1106 60-61 0.4299362900135719 1106 62-63 0.4152461856403207 1106 64-65 0.32475480081176045 1106 66-67 0.2923533519629231 1106 68-69 0.38824676727369933 1106 70-71 0.429413700081831 1106 72-73 0.4601675208933713 1106 74-75 0.4521349025836656 1106 76-77 0.46118043661954644 1106 78-79 0.4156633165522017 1106 80-81 0.42432213809941643 1106 82-83 0.4697751492986235 1106 84-85 0.4450906874102216 1106 86-87 0.33307905766137935 1106 88-89 0.18796568320795615 1106 90-91 0.20565029336509966 1106 92-93 0.13960034460023607 1106 94-95 0.12928371687638673 1106 96-97 0.18547219955721062 1106 98-99 0.22038792141192332 1106 100-101 0.36136251751397097 1107 1 -0.1887185590345659 1107 2 -0.01953545818276936 1107 3 0.04001461661050243 1107 4 -0.030215212371324185 1107 5 -0.038178869808930926 1107 6 0.037157805032180136 1107 7 -0.010266149785039147 1107 8 0.11155475754845412 1107 9 0.14015646100636303 1107 10-11 -0.0077057472406281136 1107 12-13 0.03040732065696261 1107 14-15 0.08283285299842902 1107 16-17 -0.04159769964667248 1107 18-19 0.1041030905530036 1107 20-21 0.2180517683189649 1107 22-23 0.25350236427205886 1107 24-25 0.1504034186215648 1107 26-27 0.15634907654519026 1107 28-29 0.17679224802706273 1107 30-31 0.07897326126941095 1107 32-33 0.1076535482914025 1107 34-35 0.06297739360009302 1107 36-37 0.1565525575364788 1107 38-39 0.17552317451259114 1107 40-41 0.3311773352792784 1107 42-43 -0.2129336065316636 1107 44-45 0.18528124812864633 1107 46-47 0.24765998530399713 1107 48-49 0.1210651180131137 1107 50-51 0.10477485649991536 1107 52-53 0.30243625524816053 1107 54-55 0.35919642535085217 1107 56-57 0.2564073628181589 1107 58-59 0.4447411706353037 1107 60-61 0.42616093384400244 1107 62-63 0.4786928276335942 1107 64-65 0.2808068369389005 1107 66-67 0.26800640373632945 1107 68-69 0.3567182215448099 1107 70-71 0.34117990741972903 1107 72-73 0.13970539102601975 1107 74-75 0.108506403546766 1107 76-77 0.10078496370726242 1107 78-79 -0.007079882574519303 1107 80-81 0.2635688098636102 1107 82-83 0.23543040526489278 1107 84-85 0.2861223419098273 1107 86-87 -0.010471038267127142 1107 88-89 0.2387304236284642 1107 90-91 0.31820797451603866 1107 92-93 0.011030458645748809 1107 94-95 0.04166426743478269 1107 96-97 0.21253534634309545 1107 98-99 0.12633870143538317 1107 100-101 -0.011682175756529745 1108 1 -0.092183557835952 1108 2 -0.05053518186608841 1108 3 -0.00887141779852385 1108 4 -0.1771961502416275 1108 5 -0.08413597838897857 1108 6 -0.12368408941484432 1108 7 -0.12238482268345763 1108 8 -0.09431528181504234 1108 9 -0.08909443547226203 1108 10-11 -0.16550660757479108 1108 12-13 -0.09793340541396134 1108 14-15 -0.16525859262781495 1108 16-17 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27092.0 3 6021.0 4 5130.0 5 4405.0 6 4092.0 7 4518.0 8 5299.0 9 6390.0 10 7135.0 11 8471.0 12 10483.0 13 14018.0 14 13845.0 15 11755.0 16 13565.0 17 15527.0 18 14750.0 19 12750.0 20 13191.0 21 13836.0 22 14949.0 23 16583.0 24 18954.0 25 22531.0 26 26147.0 27 31494.0 28 38186.0 29 46195.0 30 56955.0 31 69814.0 32 87903.0 33 115907.0 34 162450.0 35 247341.0 36 412998.0 37 635517.0 38 475206.0 39 63747.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.14680151699355 16.60293812194339 12.036851439502547 25.21340892156051 2 16.87333428681158 17.95721399464068 37.9764456992928 27.193006019254934 3 20.656849414021238 21.209650663296163 27.24661491361868 30.886885009063924 4 13.763810234546222 13.547260239664146 33.30775580013517 39.38117372565446 5 15.663208636122746 35.81918299491096 32.42705830254016 16.09055006642613 6 37.58916914124305 32.2100371794439 15.272501040828423 14.928292638484628 7 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25.568188212265902 24.797281133141926 30-31 24.949192866727493 24.60965583283314 25.527932261753705 24.91321903868566 32-33 24.836427161558184 24.637411802073643 25.600653402001043 24.92550763436713 34-35 25.117667565746093 24.644122480527628 25.450892213816907 24.787317739909373 36-37 25.276736924491388 24.496694033618088 25.42021697757354 24.806352064316986 38-39 25.096623078112284 24.620854687641287 25.319621850087653 24.96290038415877 40-41 25.175620605154187 24.603751593463528 25.524646329048416 24.695981472333862 42-43 25.051489550392663 24.645061233182027 25.382506826376655 24.92094239004866 44-45 25.009564539029338 24.59550844141232 25.43425195457601 24.960675064982336 46-47 25.195380851099138 24.775194624038097 25.235008674171212 24.794415850691554 48-49 25.301987290025068 24.921271654056696 24.976368273757796 24.80037278216044 50-51 25.014240721916593 25.096374530165615 25.036639193275644 24.852745554642148 52-53 25.300590447965348 24.84803829932748 24.972456400319164 24.87891485238801 54-55 25.20683981441448 24.781370986315594 24.974812325612394 25.03697687365753 56-57 24.90189850105286 25.08469915661886 25.112032641176807 24.90136970115147 58-59 25.19751285511105 24.83120600999234 24.96119762226031 25.0100835126363 60-61 25.09847131960138 24.851549995642134 25.201254919007543 24.848723765748936 62-63 25.13183454686877 24.939657501851574 25.203126031310635 24.72538191996902 64-65 24.916087035332986 25.078752176010976 25.039781839057067 24.96537894959897 66-67 24.982456869605453 25.14059572441688 24.992721127763033 24.884226278214634 68-69 24.775521800800423 24.917216882467486 25.28117446804165 25.026086848690444 70-71 24.87569844981791 24.821911933787522 25.152379934821685 25.15000968157289 72-73 24.93843692796031 25.05863648986646 24.915243080456577 25.087683501716658 74-75 24.735252725617162 25.301904626605538 24.974518718825436 24.98832392895186 76-77 25.12238497672101 25.059731454165284 24.87955699072724 24.93832657838647 78-79 24.948355668932898 25.204457577252242 24.83780060519917 25.009386148615693 80-81 25.019132726325523 25.32275819789685 24.888645435798555 24.769463639979076 82-83 25.004535931782502 25.304481828829346 24.89262834042884 24.798353898959316 84-85 24.932868466687697 25.289286489266065 24.656957226555022 25.120887817491216 86-87 24.95008589656926 25.243543807432673 24.934804859515662 24.871565436482403 88-89 24.867079215188777 25.352019175828218 24.679632321038643 25.101269287944362 90-91 24.813515639949966 25.493836270279964 24.74000000364842 24.952648086121656 92-93 24.83910136436206 25.19596482546522 24.900405636928774 25.06452817324394 94-95 24.858627462850734 25.124706150079174 25.083058416963098 24.933607970106994 96-97 25.013380731018138 24.949229787621913 24.86520827636496 25.17218120499499 98-99 24.7602989427634 25.165257017863652 24.81044476850164 25.263999270871306 100-101 24.95194177120072 25.13354673101046 24.597609749134676 25.316901748654143 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 56.0 1 56.0 2 108.5 3 184.5 4 160.0 5 137.5 6 301.0 7 564.0 8 833.5 9 985.0 10 940.5 11 962.5 12 1145.5 13 1345.5 14 1589.5 15 1881.5 16 2126.0 17 2343.0 18 2520.5 19 2589.5 20 2690.0 21 2749.0 22 2843.5 23 3124.0 24 3625.0 25 4314.5 26 5255.5 27 6573.0 28 8149.5 29 9924.0 30 11959.0 31 14772.5 32 18470.0 33 20481.5 34 21697.0 35 24376.0 36 27791.5 37 32223.5 38 37929.0 39 42789.0 40 47985.0 41 55173.0 42 61048.0 43 67178.0 44 75369.5 45 83536.0 46 90799.5 47 96972.0 48 103433.5 49 109057.5 50 112469.5 51 116285.5 52 122090.0 53 128571.5 54 132650.0 55 131027.0 56 126008.0 57 120440.0 58 116963.0 59 107804.0 60 90619.0 61 74349.0 62 60871.5 63 47880.5 64 36773.5 65 28966.5 66 21550.5 67 16972.0 68 13146.5 69 9494.5 70 7019.0 71 5058.5 72 3925.0 73 3169.5 74 2430.0 75 1780.0 76 1219.5 77 752.0 78 500.0 79 311.0 80 195.0 81 124.5 82 87.0 83 69.0 84 58.0 85 45.5 86 38.5 87 35.0 88 32.0 89 27.0 90 27.0 91 33.0 92 30.0 93 26.5 94 25.5 95 29.5 96 29.0 97 24.0 98 17.0 99 10.5 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.047075220540330615 2 0.012304163271528203 3 0.010416798993889658 4 0.005190251763505997 5 0.00816648004747447 6 0.005371729097894318 7 0.005226547230383662 8 0.009291639520682064 9 0.013864868347267769 10-11 0.0024680917476811734 12-13 0.008529434716251115 14-15 0.008801650717833596 16-17 0.018746608642313617 18-19 0.011668992601169078 20-21 0.003484364820255774 22-23 0.009690889656336372 24-25 0.021250995856872457 26-27 0.00715020697489987 28-29 0.017712187836300186 30-31 0.01395560701446193 32-33 0.01279415207437667 34-35 0.2602022020459755 36-37 0.4581758261301955 38-39 0.45547181384780944 40-41 0.4755976502314743 42-43 0.46679599951364076 44-45 0.4814956635990948 46-47 0.4865225857616513 48-49 0.49425352020659385 50-51 0.5047429101342388 52-53 0.49370908820342885 54-55 0.48086049292873567 56-57 0.4757246843655462 58-59 0.4742002747566843 60-61 0.4716958875421254 62-63 0.468284113655625 64-65 0.48403634628053127 66-67 0.45872025813336037 68-69 0.4849800284193506 70-71 0.46610638564296514 72-73 0.4739643542219794 74-75 0.4880469953705132 76-77 0.4754887638308413 78-79 0.47519840009582004 80-81 0.4767591051715596 82-83 0.4780294465122779 84-85 0.47764834411006235 86-87 0.47926349238611843 88-89 0.4932009516671415 90-91 0.5172648462070331 92-93 0.5052510466705261 94-95 0.4761602299680781 96-97 0.4506082212862025 98-99 0.44153435456678636 100-101 0.450009346082721 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2755165.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.423395490993336 #Duplication Level Percentage of deduplicated Percentage of total 1 67.89184028822869 29.480942314471097 2 14.5980947957874 12.677976874649758 3 6.2967959903346165 8.202847878432033 4 3.3105762618917645 5.7502584929288165 5 1.9781750744099615 4.2949539303264475 6 1.316378795504189 3.4296982231881503 7 0.8880165812918702 2.69924866483977 8 0.6391658158390168 2.220380000440083 9 0.4797166938045517 1.8747834946836122 >10 2.386956448611497 18.26234744933016 >50 0.1421590693881254 4.222223266220834 >100 0.0681041360244802 5.306388393085741 >500 0.0029312856443492186 0.861146883802103 >1k 0.0010887632393136518 0.7168041336013621 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3186 0.1156373574722385 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.6295466877664314E-5 2 0.0 0.0 0.0 0.0 3.6295466877664314E-5 3 0.0 0.0 0.0 0.0 3.6295466877664314E-5 4 0.0 0.0 0.0 0.0 3.6295466877664314E-5 5 0.0 0.0 0.0 0.0 7.259093375532863E-5 6 0.0 0.0 0.0 0.0 7.259093375532863E-5 7 0.0 0.0 0.0 0.0 7.259093375532863E-5 8 0.0 0.0 0.0 0.0 7.259093375532863E-5 9 0.0 0.0 0.0 0.0 7.259093375532863E-5 10-11 0.0 0.0 0.0 0.0 1.0888640063299294E-4 12-13 0.0 0.0 0.0 0.0 4.5369333597080393E-4 14-15 0.0 0.0 0.0 0.0 5.988752034814612E-4 16-17 0.0 0.0 0.0 0.0 6.714661372367898E-4 18-19 0.0 0.0 0.0 3.6295466877664314E-5 8.710912050639435E-4 20-21 0.0 0.0 0.0 1.8147733438832157E-4 9.799776056969365E-4 22-23 0.0 0.0 0.0 2.7221600158248234E-4 0.001034420806013433 24-25 0.0 0.0 0.0 3.992501356543074E-4 0.001052568539452265 26-27 0.0 0.0 0.0 5.081365362873003E-4 0.0010888640063299294 28-29 0.0 0.0 0.0 9.436821388192722E-4 0.0011070117397687616 30-31 0.0 0.0 0.0 0.002413648547364677 0.0011251594732075937 32-33 0.0 0.0 0.0 0.005262842697261325 0.001161454940085258 34-35 0.0 0.0 0.0 0.008874241651588925 0.001270341340718251 36-37 0.0 0.0 0.0 0.015098914221108355 0.001270341340718251 38-39 0.0 0.0 0.0 0.025025724412149543 0.0013792277413512439 40-41 0.0 0.0 0.0 0.037456921817749574 0.0013792277413512439 42-43 0.0 0.0 0.0 0.050559585360586384 0.0014155232082289082 44-45 0.0 0.0 0.0 0.06520480624572395 0.0015425573423007333 46-47 0.0 0.0 0.0 0.08376993755364924 0.001633296009494894 48-49 0.0 0.0 0.0 0.10500278567708286 0.0017058869432502227 50-51 0.0 0.0 0.0 0.1274878274077959 0.0017784778770055514 52-53 0.0 0.0 0.0 0.15004546007226427 0.0020325461451492018 54-55 0.0 0.0 0.0 0.17556117328726228 0.0020325461451492018 56-57 0.0 0.0 0.0 0.2046338422562714 0.0021051370789045305 58-59 0.0 0.0 0.0 0.23936860405819615 0.0021777280126598587 60-61 3.6295466877664314E-5 3.6295466877664314E-5 0.0 0.2791847312229939 0.0021777280126598587 62-63 3.6295466877664314E-5 3.6295466877664314E-5 0.0 0.32045267706289826 0.0023592053470481806 64-65 3.6295466877664314E-5 3.6295466877664314E-5 0.0 0.36230135037284517 0.002413648547364677 66-67 3.6295466877664314E-5 3.6295466877664314E-5 0.0 0.41351425413722953 0.002449944014242341 68-69 3.6295466877664314E-5 3.6295466877664314E-5 0.0 0.4722040240784127 0.0024680917476811734 70-71 3.6295466877664314E-5 3.6295466877664314E-5 0.0 0.5387154671317326 0.0024680917476811734 72-73 3.6295466877664314E-5 3.6295466877664314E-5 0.0 0.6083483203365315 0.0025951258817529985 74-75 3.6295466877664314E-5 3.6295466877664314E-5 0.0 0.6771645255365831 0.00277660321614132 76-77 3.6295466877664314E-5 3.6295466877664314E-5 0.0 0.7546734950538352 0.0028310464164578164 78-79 3.6295466877664314E-5 3.6295466877664314E-5 0.0 0.8474447083931452 0.0029217850836519774 80-81 3.6295466877664314E-5 3.6295466877664314E-5 0.0 0.9518849143336243 0.002958080550529642 82-83 3.6295466877664314E-5 3.6295466877664314E-5 0.0 1.0623683155092345 0.0030851146846014666 84-85 3.6295466877664314E-5 3.6295466877664314E-5 0.0 1.1775519796455023 0.0030851146846014666 86-87 3.6295466877664314E-5 3.6295466877664314E-5 0.0 1.2985247707487573 0.003230296552112124 88-89 3.6295466877664314E-5 3.6295466877664314E-5 0.0 1.4309669293853544 0.0033754784196227813 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2630 0.0 43.03144 1 GTATCAA 4520 0.0 41.066845 1 TCAACGC 5735 0.0 31.857834 4 ATCAACG 5855 0.0 31.04547 3 CAACGCA 6010 0.0 30.2426 5 AACGCAG 6110 0.0 29.977314 6 TATCAAC 6180 0.0 29.720284 2 GTACATG 9495 0.0 29.47381 1 TACATGG 9570 0.0 28.39289 2 ACATGGG 9760 0.0 26.772146 3 ACGCAGA 7555 0.0 24.056232 7 CATGGGG 7715 0.0 23.559044 4 CGCAGAG 7975 0.0 22.730799 8 GAGTACA 6925 0.0 22.70189 1 TACCTGG 2540 0.0 22.178026 2 GTACCTG 2670 0.0 21.814114 1 ATCGCGT 110 0.0029384838 21.514135 8 GTGGTAT 1715 0.0 21.260345 1 AGTACAT 6745 0.0 20.984514 2 GTGTAGG 1275 0.0 19.68382 1 >>END_MODULE