FastQCFastQC Report
Thu 26 May 2016
SRR936361_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936361_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2755165
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35700.1295748167532616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30150.042.7539561
GTATCAA52200.041.642811
TCAACGC65100.033.0086444
ATCAACG65750.032.826023
CAACGCA69050.031.1890875
AACGCAG69500.031.1236526
TATCAAC70050.030.9520532
GTACATG93200.028.1209531
TACATGG95800.026.4458272
ACGCAGA83050.025.7600887
ACATGGG95400.025.557443
CGCAGAG86200.024.8187438
CATGGGG74450.022.3641744
TACCTGG24650.021.364242
GTACCTG26050.021.180911
GCAGAGT104200.020.258299
GAGTACA69850.020.156711
AGAGTAC86650.018.74988410-11
TAAGGTG8750.018.4323775
AGTACAT68800.018.4121382
Produced by FastQC (version 0.11.5)