##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936361_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2755165 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46519827306169 34.0 31.0 34.0 31.0 34.0 2 32.599634867603214 34.0 31.0 34.0 31.0 34.0 3 32.68070151878381 34.0 31.0 34.0 31.0 34.0 4 36.176797396889114 37.0 35.0 37.0 35.0 37.0 5 36.12548322877214 37.0 35.0 37.0 35.0 37.0 6 36.15910952701562 37.0 36.0 37.0 35.0 37.0 7 36.10334226806743 37.0 36.0 37.0 35.0 37.0 8 36.078784392223334 37.0 36.0 37.0 35.0 37.0 9 37.843068563951704 39.0 38.0 39.0 35.0 39.0 10-11 37.77125344580088 39.0 38.0 39.0 35.0 39.0 12-13 37.70673099433246 39.0 37.5 39.0 35.0 39.0 14-15 39.07250890600019 40.0 38.0 41.0 36.0 41.0 16-17 38.99276758379263 40.0 38.0 41.0 35.0 41.0 18-19 38.983818936433934 40.0 38.0 41.0 35.0 41.0 20-21 38.922195222427696 40.0 38.0 41.0 35.0 41.0 22-23 38.802007139318334 40.0 38.0 41.0 35.0 41.0 24-25 38.955248959681185 40.0 38.0 41.0 35.0 41.0 26-27 38.87452366736656 40.0 38.0 41.0 35.0 41.0 28-29 38.780195922930204 40.0 38.0 41.0 35.0 41.0 30-31 38.67562886433299 40.0 38.0 41.0 34.5 41.0 32-33 38.540376529173386 40.0 38.0 41.0 34.0 41.0 34-35 38.407732930695616 40.0 38.0 41.0 34.0 41.0 36-37 38.267250237281615 40.0 38.0 41.0 34.0 41.0 38-39 38.130927004371785 40.0 38.0 41.0 33.0 41.0 40-41 37.979879970891034 40.0 37.5 41.0 33.0 41.0 42-43 38.02021657505086 40.0 37.5 41.0 33.0 41.0 44-45 37.901709335012605 40.0 37.0 41.0 33.0 41.0 46-47 37.71615983071794 40.0 37.0 41.0 33.0 41.0 48-49 37.49836507069449 40.0 37.0 41.0 32.5 41.0 50-51 37.2754410352919 40.0 36.0 41.0 32.0 41.0 52-53 37.02921748788185 39.0 36.0 41.0 31.5 41.0 54-55 36.79053631996632 39.0 35.0 41.0 31.0 41.0 56-57 36.49025394123401 39.0 35.0 41.0 31.0 41.0 58-59 36.18050570474001 38.0 35.0 40.0 30.5 41.0 60-61 35.84889253456689 37.5 35.0 40.0 30.0 41.0 62-63 35.500807937092695 37.0 35.0 40.0 30.0 41.0 64-65 35.08907905697118 36.5 34.0 39.5 29.0 41.0 66-67 34.70949235345252 36.0 34.0 39.0 29.0 41.0 68-69 34.33187504196663 35.5 34.0 39.0 28.5 40.0 70-71 33.95305653200444 35.0 34.0 37.5 28.0 40.0 72-73 33.55654652262206 35.0 33.0 37.0 27.5 39.0 74-75 33.1437567260037 35.0 33.0 36.5 27.0 39.0 76-77 32.47550963372429 35.0 32.0 36.0 26.0 38.0 78-79 32.56569025811521 35.0 33.0 35.5 26.0 37.0 80-81 32.4388350606951 35.0 33.0 35.0 26.0 37.0 82-83 32.19812715390911 35.0 33.0 35.0 26.0 36.5 84-85 31.954094219402467 35.0 33.0 35.0 26.0 36.0 86-87 31.585401963221805 35.0 32.0 35.0 25.0 36.0 88-89 31.116670326459577 34.5 32.0 35.0 23.5 35.0 90-91 30.919247486085226 34.0 31.5 35.0 23.0 35.0 92-93 30.636719760885462 34.0 31.0 35.0 20.0 35.0 94-95 30.47750261781055 34.0 31.0 35.0 20.0 35.0 96-97 30.217475722869594 34.0 31.0 35.0 18.0 35.0 98-99 29.72902548486207 34.0 31.0 35.0 2.0 35.0 100-101 26.32500630633737 30.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05815930194481922 1101 2 0.14387562655760888 1101 3 0.1330713992382897 1101 4 0.1062878551858546 1101 5 0.032361761554348334 1101 6 0.08874605583523021 1101 7 0.04879504882607222 1101 8 0.0017891674291945492 1101 9 0.1464157574608933 1101 10-11 0.10724646356624845 1101 12-13 0.21639767385168795 1101 14-15 0.26322620271272257 1101 16-17 0.3007339613491169 1101 18-19 0.23303400629406212 1101 20-21 0.19984331634276487 1101 22-23 0.2297068777054747 1101 24-25 0.16483297095108895 1101 26-27 0.1362513852691336 1101 28-29 0.1982927373465344 1101 30-31 0.2285176607211028 1101 32-33 0.22078582954348036 1101 34-35 0.16647231041208954 1101 36-37 0.23418285379882775 1101 38-39 0.22060630270334514 1101 40-41 0.23387938322083102 1101 42-43 0.22359706373259058 1101 44-45 0.24533277412106713 1101 46-47 0.19653414085002652 1101 48-49 0.10544390397288339 1101 50-51 0.03939606773236193 1101 52-53 0.1343109250332617 1101 54-55 0.06276825711076839 1101 56-57 0.047020928161686015 1101 58-59 0.003146980097533003 1101 60-61 0.035922183587473455 1101 62-63 0.02380229431728509 1101 64-65 0.031437382059806396 1101 66-67 0.07617491675112831 1101 68-69 0.06681622949302124 1101 70-71 0.2083261684966189 1101 72-73 0.21914148256241361 1101 74-75 0.16736271016869608 1101 76-77 -0.027233706420922488 1101 78-79 -0.022873149934561354 1101 80-81 0.11097520331155408 1101 82-83 0.03992770971000681 1101 84-85 0.14698485936928307 1101 86-87 0.1797992364863532 1101 88-89 0.0780608733344117 1101 90-91 0.2534076909694285 1101 92-93 0.34341027329288565 1101 94-95 0.266230698742735 1101 96-97 0.12229178212067637 1101 98-99 0.11129433118581034 1101 100-101 0.5289204519480002 1102 1 -6.386377293026158E-4 1102 2 0.11559177160309275 1102 3 0.09414810763043846 1102 4 0.124727919230601 1102 5 0.10197567507147909 1102 6 0.12661573980207663 1102 7 0.11528464015125195 1102 8 0.1743279477011086 1102 9 0.08128476546227859 1102 10-11 0.10429534436259047 1102 12-13 0.19064386356142649 1102 14-15 0.1496626889838879 1102 16-17 0.19445050088327065 1102 18-19 0.17324326428333592 1102 20-21 0.12304095235887758 1102 22-23 0.13528931355367746 1102 24-25 0.07607258140405548 1102 26-27 0.15627501949249734 1102 28-29 0.15274614240848194 1102 30-31 0.14775917903919833 1102 32-33 0.09967622837714174 1102 34-35 0.09922945279792827 1102 36-37 -0.011770478612625368 1102 38-39 -0.0631912684537781 1102 40-41 -0.06043829527651923 1102 42-43 -0.04357416119685098 1102 44-45 -0.033850701015268214 1102 46-47 0.06954055164189299 1102 48-49 0.05101780426551983 1102 50-51 -0.04099766986528408 1102 52-53 -0.09079838412723262 1102 54-55 -0.07055110182408697 1102 56-57 0.08651524562960589 1102 58-59 -0.0954854533289975 1102 60-61 -0.08025551951979537 1102 62-63 0.044770648183451556 1102 64-65 0.06483660389229584 1102 66-67 0.003096241531125088 1102 68-69 -0.0739178668927778 1102 70-71 -0.0841010178094237 1102 72-73 -0.18417185375181333 1102 74-75 -0.2013766247739497 1102 76-77 -0.23224041343025448 1102 78-79 -0.2597948327025037 1102 80-81 -0.12053612349704679 1102 82-83 -0.05775517825590981 1102 84-85 -0.17358237252057052 1102 86-87 -0.20915597571275413 1102 88-89 -0.013478397591558888 1102 90-91 0.09228583047100969 1102 92-93 0.09479440558276764 1102 94-95 0.016386090076199622 1102 96-97 -0.2231148114556163 1102 98-99 -0.11881250715596181 1102 100-101 0.24884332309551027 1103 1 0.018304635332590635 1103 2 0.049554264873655995 1103 3 0.1268578804555318 1103 4 0.07090087817126545 1103 5 0.07110325704914544 1103 6 0.11753758121815139 1103 7 0.2077190694884834 1103 8 0.17153868996950905 1103 9 0.06649687588031838 1103 10-11 0.18987350901817734 1103 12-13 0.18172474899980529 1103 14-15 0.2980865171853111 1103 16-17 0.2974688629625888 1103 18-19 0.2182625047309088 1103 20-21 0.20429355811725003 1103 22-23 0.2697754546296096 1103 24-25 0.19653400808459054 1103 26-27 0.16571343049598397 1103 28-29 0.19695161041955345 1103 30-31 0.24913802156302012 1103 32-33 0.23919747045192707 1103 34-35 0.22577030771605422 1103 36-37 0.23637901129962557 1103 38-39 0.2573210590602031 1103 40-41 0.29875326861027673 1103 42-43 0.34721960171388133 1103 44-45 0.3407769032251835 1103 46-47 0.34561331453507904 1103 48-49 0.3341907105850481 1103 50-51 0.42126217872075955 1103 52-53 0.42123060607309526 1103 54-55 0.3544642797398936 1103 56-57 0.3478028174608383 1103 58-59 0.4616531777388033 1103 60-61 0.3990628545679371 1103 62-63 0.44267096350551327 1103 64-65 0.5001250824262442 1103 66-67 0.4429870459194589 1103 68-69 0.39470863365300346 1103 70-71 0.33826289920029495 1103 72-73 0.3540983881839921 1103 74-75 0.4310738202794724 1103 76-77 0.29799886433784906 1103 78-79 0.3507335105276539 1103 80-81 0.45891201567103224 1103 82-83 0.4776809430533575 1103 84-85 0.49452918294683457 1103 86-87 0.3625657947322445 1103 88-89 0.4801905371811621 1103 90-91 0.7582844447809904 1103 92-93 0.7859071335571102 1103 94-95 0.6486906463922892 1103 96-97 0.4048345022432862 1103 98-99 0.24492319574778065 1103 100-101 0.6517448392589706 1104 1 -0.020380449283194935 1104 2 0.0037512645984563164 1104 3 0.007563910637792048 1104 4 -0.06872391254135124 1104 5 -0.031012814043556602 1104 6 0.04278016318917821 1104 7 -0.005437607810016232 1104 8 0.060427468866350864 1104 9 0.0028903420884489606 1104 10-11 0.04132830789441044 1104 12-13 0.08315146690517139 1104 14-15 0.08230207476912454 1104 16-17 0.1842578385912077 1104 18-19 0.2063271021831028 1104 20-21 0.10040996974534977 1104 22-23 0.1163937929884824 1104 24-25 0.13198968205758632 1104 26-27 0.21805193851539428 1104 28-29 0.17056836687273602 1104 30-31 0.1320352584086848 1104 32-33 0.1061913414748048 1104 34-35 0.1639436653092332 1104 36-37 0.11091217753303795 1104 38-39 0.26193016600613106 1104 40-41 0.3837562812925768 1104 42-43 0.35884301180626466 1104 44-45 0.31392162878731966 1104 46-47 0.3541083856812435 1104 48-49 0.24407768715211375 1104 50-51 0.33767971338970426 1104 52-53 0.29918776988843376 1104 54-55 0.16476973206791 1104 56-57 0.33327677211287465 1104 58-59 0.38652090679669726 1104 60-61 0.2821755658434171 1104 62-63 0.2872398199599431 1104 64-65 0.1662703867665556 1104 66-67 0.18674769177518868 1104 68-69 0.3389070258771838 1104 70-71 0.2603586635603605 1104 72-73 0.2256249766727123 1104 74-75 0.36035283557782094 1104 76-77 0.2868568894569563 1104 78-79 0.23911594101323175 1104 80-81 0.311277713725687 1104 82-83 0.2661739815463946 1104 84-85 0.3601615723503784 1104 86-87 0.29403288192052557 1104 88-89 0.4788415946557514 1104 90-91 0.5443666774788554 1104 92-93 0.6624142171552485 1104 94-95 0.6289605470892674 1104 96-97 0.4633828315410078 1104 98-99 0.24492352572393727 1104 100-101 0.2318298873133955 1105 1 -0.008630593038617462 1105 2 0.07972580421950681 1105 3 0.06911260571783373 1105 4 0.047709140180970167 1105 5 -0.03848375680591687 1105 6 0.002353952871381182 1105 7 0.08996311866512485 1105 8 0.0688205677061191 1105 9 0.05456386733781926 1105 10-11 0.11421238718633475 1105 12-13 0.09765686307711263 1105 14-15 0.10198555891841465 1105 16-17 0.19922559848478016 1105 18-19 0.1842274180432497 1105 20-21 0.12738849916193118 1105 22-23 0.04827003507872263 1105 24-25 0.06402567025116213 1105 26-27 0.08612399590056441 1105 28-29 9.86226679934532E-4 1105 30-31 -0.01073123232298201 1105 32-33 -0.03710766305304958 1105 34-35 0.025735483030253192 1105 36-37 0.1748356896845067 1105 38-39 0.05891247533051569 1105 40-41 0.13985853422691008 1105 42-43 0.19973460720056124 1105 44-45 0.042361851120467975 1105 46-47 0.10805856611077047 1105 48-49 0.08807549249137026 1105 50-51 0.07062537046528661 1105 52-53 0.1737069646523679 1105 54-55 0.07728117866368933 1105 56-57 0.1269613047365894 1105 58-59 0.06475360383483775 1105 60-61 0.10678197812271861 1105 62-63 0.20465534335985325 1105 64-65 0.11160515770767887 1105 66-67 0.14567855964008913 1105 68-69 0.22376254886567182 1105 70-71 0.09458728755499379 1105 72-73 -0.023535392022573376 1105 74-75 0.05909619301663582 1105 76-77 0.11473665046136006 1105 78-79 0.12696244461458406 1105 80-81 0.18076241522386738 1105 82-83 0.2446342926752365 1105 84-85 0.21124710658538248 1105 86-87 0.302594565933731 1105 88-89 0.4617658772527129 1105 90-91 0.441881838091188 1105 92-93 0.5565183002592242 1105 94-95 0.4287010480620417 1105 96-97 0.3257120647243603 1105 98-99 0.40701985744555813 1105 100-101 0.6004147714749699 1106 1 0.008578364736749222 1106 2 0.04940356363599818 1106 3 0.07489997251936131 1106 4 0.04126646538468037 1106 5 0.01973893394568904 1106 6 0.06952302233402463 1106 7 0.03080376485578995 1106 8 0.04145077455550705 1106 9 -0.03385997447653466 1106 10-11 0.08844346872944442 1106 12-13 0.11490321614567023 1106 14-15 0.1797410578072416 1106 16-17 0.03472014233342691 1106 18-19 0.08508537291247364 1106 20-21 0.09783553318096239 1106 22-23 0.0820629150148946 1106 24-25 0.12952441661848013 1106 26-27 -0.016689910874745806 1106 28-29 0.04861358436414065 1106 30-31 0.10034169206016941 1106 32-33 0.12172617363280125 1106 34-35 0.15759484823624348 1106 36-37 0.14662005061675387 1106 38-39 0.314977635738515 1106 40-41 0.07944020554720765 1106 42-43 0.12260592465455744 1106 44-45 0.06928290075292409 1106 46-47 0.09912999468219397 1106 48-49 0.05412946992598222 1106 50-51 0.06179473909027422 1106 52-53 0.10755647174678273 1106 54-55 0.23217070228327685 1106 56-57 0.2923856166631964 1106 58-59 0.20109328130933335 1106 60-61 0.21716932213092122 1106 62-63 0.1560877611614373 1106 64-65 0.20722848382312264 1106 66-67 0.26600592872095064 1106 68-69 0.24615520656696788 1106 70-71 0.19848209637601144 1106 72-73 0.05103438403303784 1106 74-75 0.1539003841386375 1106 76-77 0.05807565838203743 1106 78-79 -0.08045500082389623 1106 80-81 0.03795513275763085 1106 82-83 0.1320513107389374 1106 84-85 0.1306602497844267 1106 86-87 0.10221811998239616 1106 88-89 0.11900418142959168 1106 90-91 0.3119385364785643 1106 92-93 0.3695614223744421 1106 94-95 0.3759131399191311 1106 96-97 0.3171449811298537 1106 98-99 0.24459429188626913 1106 100-101 0.4970702076821638 1107 1 -0.018770166183017523 1107 2 -9.713081836650872E-4 1107 3 0.01863468947937008 1107 4 0.010787694338297626 1107 5 -0.07178437006625416 1107 6 -0.02941502108062366 1107 7 -0.07072158113570026 1107 8 -0.04629439021506698 1107 9 -0.018336477080850955 1107 10-11 -0.04426015352596835 1107 12-13 0.038301550373823545 1107 14-15 0.04077472884115707 1107 16-17 0.022299769993416874 1107 18-19 0.04752432026884179 1107 20-21 0.021441643597988502 1107 22-23 -0.09478626921109878 1107 24-25 0.10914358425418413 1107 26-27 0.12170580885624815 1107 28-29 0.1543510697649353 1107 30-31 -0.03437130803397537 1107 32-33 2.802358362075097E-4 1107 34-35 0.03882288582393301 1107 36-37 -0.05569996574452318 1107 38-39 0.03114900044241864 1107 40-41 0.0172346545435218 1107 42-43 -0.07703121049268447 1107 44-45 -0.1122018473774844 1107 46-47 -0.1559903803295697 1107 48-49 -0.21830662120355981 1107 50-51 -0.08253798843458782 1107 52-53 0.030401133719657025 1107 54-55 0.06715704773786513 1107 56-57 -0.13566140166499707 1107 58-59 -0.08969382864712117 1107 60-61 -0.028931673795298707 1107 62-63 -0.0057284619524935465 1107 64-65 -0.08114665707982027 1107 66-67 -0.23391805366077278 1107 68-69 -0.053155975211197415 1107 70-71 -0.03419860927105134 1107 72-73 -0.044535665407046565 1107 74-75 -0.03058093715571175 1107 76-77 -0.1283463386357866 1107 78-79 -0.15891893921082811 1107 80-81 -0.0013765101643983257 1107 82-83 0.11111457966192262 1107 84-85 0.06089030963160269 1107 86-87 0.07702297098786559 1107 88-89 0.14534158260668661 1107 90-91 -0.2236036758822202 1107 92-93 0.11915692276756928 1107 94-95 0.11598345780636166 1107 96-97 -0.12541249645926555 1107 98-99 -0.14445616536447758 1107 100-101 0.2234442340841305 1108 1 -0.12063628067115673 1108 2 -0.08383530114270599 1108 3 -0.04703998685641153 1108 4 -0.005080822337305335 1108 5 -0.035732640854128306 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-0.8928420833547008 2316 62-63 -0.9014282676904983 2316 64-65 -0.6859270390383756 2316 66-67 -0.7409426406508217 2316 68-69 -0.7461710803069295 2316 70-71 -0.6590441943100629 2316 72-73 -0.8420951517863031 2316 74-75 -0.7353212878798487 2316 76-77 -0.5921331577524924 2316 78-79 -0.7272313110141013 2316 80-81 -0.7100250811849875 2316 82-83 -0.8797009827699043 2316 84-85 -0.862378713712701 2316 86-87 -0.9609726698995686 2316 88-89 -0.866614401045414 2316 90-91 -0.9463899503729927 2316 92-93 -0.9911303560124942 2316 94-95 -0.8995878503589445 2316 96-97 -0.7702348135625421 2316 98-99 -0.937350077447558 2316 100-101 -1.0286246603369058 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 19.0 5 46.0 6 98.0 7 218.0 8 521.0 9 855.0 10 1277.0 11 2181.0 12 3037.0 13 4356.0 14 5660.0 15 7415.0 16 8735.0 17 11567.0 18 11422.0 19 8721.0 20 8507.0 21 9285.0 22 10125.0 23 11627.0 24 14171.0 25 17225.0 26 21643.0 27 26948.0 28 33838.0 29 42155.0 30 52722.0 31 65462.0 32 82923.0 33 107750.0 34 151653.0 35 230321.0 36 406327.0 37 714806.0 38 602236.0 39 79293.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.35235736434588 16.988329173282175 12.495765044871419 25.163548417500525 2 17.120728085906585 18.21086030015029 37.62508077339161 27.043330840551512 3 20.69139234855263 21.274442728475425 27.713766689109363 30.320398233862583 4 14.057379503586901 13.920618184391861 33.18098916035881 38.84101315166242 5 15.521720114766266 35.77328399569536 32.65027684367361 16.054719045864765 6 37.494886876103614 32.46923505488782 15.095792810956876 14.940085258051697 7 32.645885092181416 30.07634751457717 18.141853573197974 19.135913820043445 8 29.16177434019378 34.187353570475814 17.486684100589255 19.164187988741148 9 27.443292869937007 15.248814499313108 18.08650298620954 39.221389644540345 10-11 27.792629716221406 24.49234771792621 25.15889739192863 22.55612517392375 12-13 27.304555120695216 21.25966775831412 26.439325005453394 24.996452115537267 14-15 23.949763444294625 22.98838726537249 23.505379895577942 29.55646939475494 16-17 25.037094169101497 26.163366785837432 24.195506596737022 24.60403244832405 18-19 24.982006522295396 24.947779897029758 25.650750499516363 24.41946308115848 20-21 25.273962870080126 24.26447650021496 25.7822267172166 24.679333912488318 22-23 25.121417410572505 24.042716134968323 25.841864280360703 24.994002174098465 24-25 24.949715862637692 24.32532688999484 25.808851859292613 24.916105388074854 26-27 24.850613136752088 24.602527317569812 25.80794854623888 24.73891099943922 28-29 24.80589626724847 24.713558387557036 25.782975493802585 24.69756985139191 30-31 24.820716133696873 24.606805926984155 25.727770179824677 24.844707759494295 32-33 24.656380728175048 24.540178914134607 25.86044784611054 24.9429925115798 34-35 25.016759760314557 24.570861210310582 25.716677113916642 24.695701915458216 36-37 25.088706153245322 24.523621824326973 25.66455209199888 24.723119930428826 38-39 25.000095275721584 24.560211806095577 25.58078718797428 24.858905730208555 40-41 25.083403005974453 24.53873142498956 25.69718161992475 24.680683949111238 42-43 24.985812445520608 24.658740994509913 25.511790960291 24.84365559967848 44-45 24.891812720628096 24.523054938357976 25.652685304006933 24.93244703700699 46-47 25.059206609650204 24.71986670345148 25.41063468323919 24.81029200365912 48-49 25.141614509776662 24.857359988630545 25.17163538826307 24.829390113329726 50-51 24.92171910347888 25.007255037010218 25.21821826454433 24.852807594966574 52-53 25.19783945144528 24.78741435638452 25.149526388605814 24.865219803564386 54-55 25.142106342474378 24.72563249617535 25.13845863481365 24.99380252653662 56-57 24.82626563119313 25.115931802772202 25.20355214462946 24.854250421405208 58-59 25.16143527217782 24.78677041982426 25.178639548275232 24.873154759722684 60-61 24.964280276378922 24.828460938601637 25.365822054526998 24.84143673049244 62-63 25.064397232107694 24.835499870243705 25.369986915484187 24.730115982164406 64-65 24.8861717841045 24.997291424732367 25.169696550645277 24.946840240517854 66-67 24.9378893823056 24.956853763749177 25.206639166801263 24.898617687143965 68-69 24.73998834915513 24.83874831452926 25.539196382067864 24.88206695424775 70-71 24.747982151321775 24.816671397181 25.339780383149886 25.095566068347342 72-73 24.968123089699247 24.985526993472902 25.013801531616124 25.03254838521173 74-75 24.58682147893139 25.20765543987384 25.17449953088109 25.031023550313687 76-77 25.076310644871313 25.000036364376875 25.021800443936314 24.901852546815498 78-79 24.858926919939087 25.15585116983068 25.01252095682091 24.972700953409323 80-81 24.918860453045774 25.21875555781397 25.046803599104216 24.81558039003604 82-83 24.939631564715725 25.20433440465453 25.109476201969755 24.74655782865999 84-85 24.870383480515464 25.21631789815895 24.783273775096895 25.130024846228682 86-87 24.87455232299645 25.083521252745207 25.098711064524178 24.94321535973417 88-89 24.80244563649997 25.282778545628982 24.823692359138246 25.091083458732804 90-91 24.80113005457319 25.419110446087583 24.860090215052587 24.919669284286634 92-93 24.87493270227267 25.16405356735288 24.967229328449676 24.993784401924778 94-95 24.77550355965759 25.032426104143557 25.153709805831742 25.03836053036711 96-97 24.959615458953678 24.861941220143976 24.961502883858216 25.216940437044126 98-99 24.715366032884422 25.071654168210035 24.879408843357538 25.333570955548012 100-101 24.764072830405564 25.063186432779016 24.855084522912716 25.3176562139027 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 49.0 1 47.0 2 95.0 3 152.0 4 117.5 5 110.5 6 242.0 7 475.0 8 759.0 9 881.5 10 779.5 11 706.5 12 777.0 13 895.0 14 1026.0 15 1139.0 16 1237.5 17 1362.0 18 1510.5 19 1657.5 20 1813.0 21 2092.0 22 2409.0 23 2882.5 24 3644.0 25 4519.5 26 5549.0 27 6854.0 28 8448.5 29 10341.0 30 12498.5 31 15296.0 32 18759.5 33 20852.0 34 22059.0 35 24676.5 36 28271.5 37 32698.5 38 38636.0 39 43448.5 40 48406.5 41 55640.0 42 61854.5 43 68057.5 44 75666.5 45 83508.0 46 90852.0 47 97695.5 48 104295.5 49 109192.0 50 112834.0 51 116889.5 52 122659.0 53 129721.0 54 133611.0 55 131515.0 56 127321.0 57 122314.5 58 118306.0 59 108375.5 60 89837.0 61 72960.5 62 59962.5 63 47079.5 64 36066.0 65 28418.0 66 21034.0 67 16379.5 68 12602.5 69 8953.0 70 6479.0 71 4546.5 72 3472.0 73 2856.5 74 2146.0 75 1525.0 76 998.5 77 585.5 78 377.5 79 209.0 80 100.5 81 45.0 82 23.0 83 19.0 84 14.5 85 7.0 86 5.5 87 2.0 88 0.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2613999524529384 2 0.01978102944832705 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.3592053470481802E-4 12-13 7.259093375532863E-5 14-15 0.0 16-17 5.444320031649647E-4 18-19 0.0 20-21 5.444320031649647E-5 22-23 0.0 24-25 0.0025951258817529985 26-27 0.002540682681436502 28-29 0.0023955008139258448 30-31 0.0013429322744735795 32-33 0.0016151482760560622 34-35 0.0019599552113938727 36-37 3.6295466877664314E-5 38-39 1.270341340718251E-4 40-41 6.714661372367898E-4 42-43 0.003974353623104242 44-45 0.0037928762887159207 46-47 0.014699664085454048 48-49 0.015080766487669522 50-51 0.006623922705173737 52-53 0.007857968579014324 54-55 3.6295466877664314E-4 56-57 0.0035206602871334387 58-59 0.0013066368075959154 60-61 0.001252193607279419 62-63 0.0 64-65 9.618298722581043E-4 66-67 0.0 68-69 0.0 70-71 1.0888640063299294E-4 72-73 0.0013066368075959154 74-75 0.0 76-77 0.1894986325682854 78-79 0.10088325018646797 80-81 0.001270341340718251 82-83 0.0 84-85 6.714661372367898E-4 86-87 0.12051909776728435 88-89 0.5777875372255382 90-91 0.3047004444379919 92-93 0.2919607355639317 94-95 0.001978102944832705 96-97 0.003212148818673292 98-99 0.003321035219306285 100-101 8.892389385027757E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2755165.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.15182572840512 #Duplication Level Percentage of deduplicated Percentage of total 1 65.2097742551557 26.83501265936806 2 15.81247988506008 13.014248331278077 3 6.747611999113923 8.330296592112944 4 3.659663918011839 6.024074071142221 5 2.124669624229625 4.371701705336676 6 1.4004864501732133 3.4579544599512486 7 0.9655871537615405 2.781497199402715 8 0.6897892534703475 2.2708869718510725 9 0.508999034545518 1.8851615608989254 >10 2.6400384357062467 19.109337895171187 >50 0.15707416087115642 4.420640606720919 >100 0.07984750513147074 5.935233612812249 >500 0.002740623729995098 0.7583293974378139 >1k 0.0012377010393404837 0.8056249365157805 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3570 0.1295748167532616 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.6295466877664314E-5 2 0.0 0.0 0.0 0.0 3.6295466877664314E-5 3 0.0 0.0 0.0 0.0 3.6295466877664314E-5 4 0.0 0.0 0.0 0.0 3.6295466877664314E-5 5 0.0 0.0 0.0 0.0 3.6295466877664314E-5 6 0.0 0.0 0.0 0.0 3.6295466877664314E-5 7 0.0 0.0 0.0 0.0 3.6295466877664314E-5 8 0.0 0.0 0.0 0.0 3.6295466877664314E-5 9 0.0 0.0 0.0 0.0 3.6295466877664314E-5 10-11 0.0 0.0 0.0 0.0 7.259093375532863E-5 12-13 0.0 0.0 0.0 0.0 4.1739786909313965E-4 14-15 0.0 0.0 0.0 1.0888640063299294E-4 5.262842697261326E-4 16-17 0.0 0.0 0.0 1.0888640063299294E-4 5.625797366037968E-4 18-19 0.0 0.0 0.0 1.2703413407182508E-4 6.714661372367898E-4 20-21 0.0 0.0 0.0 2.5406826814365017E-4 7.98500271308615E-4 22-23 0.0 0.0 0.0 3.44806935337811E-4 7.98500271308615E-4 24-25 0.0 0.0 0.0 4.718410694096361E-4 7.98500271308615E-4 26-27 0.0 0.0 0.0 5.80727470042629E-4 9.2553440538044E-4 28-29 0.0 0.0 0.0 0.0010162730725746009 9.436821388192722E-4 30-31 0.0 0.0 0.0 0.00252253494799767 9.618298722581044E-4 32-33 0.0 0.0 0.0 0.005426172298210815 0.001052568539452265 34-35 0.0 0.0 0.0 0.009110162186293742 0.0011251594732075937 36-37 0.0 0.0 0.0 0.015443721156446166 0.0011251594732075937 38-39 0.0 0.0 0.0 0.025515713214998015 0.0011796026735240903 40-41 0.0 0.0 0.0 0.038146535688425194 0.0011977504069629224 42-43 0.0 0.0 0.0 0.05132179016501734 0.0012340458738405867 44-45 0.0 0.0 0.0 0.06609404518422671 0.0013066368075959154 46-47 0.0 0.0 0.0 0.08482250609310149 0.0014336709416677405 48-49 0.0 0.0 0.0 0.10630942248467878 0.0014881141419842369 50-51 0.0 0.0 0.0 0.12892149834946365 0.0014881141419842369 52-53 0.0 0.0 0.0 0.15146098328049318 0.00159700054261723 54-55 0.0 0.0 0.0 0.1773033556973902 0.00159700054261723 56-57 0.0 0.0 0.0 0.2064486156001546 0.001615148276056062 58-59 0.0 0.0 0.0 0.2411652296686405 0.001633296009494894 60-61 0.0 0.0 0.0 0.2806365498981005 0.001633296009494894 62-63 0.0 0.0 0.0 0.32204967760551545 0.001633296009494894 64-65 0.0 0.0 0.0 0.36407982824985075 0.0016695914763725584 66-67 0.0 0.0 0.0 0.4156919821498894 0.0016695914763725584 68-69 0.0 0.0 0.0 0.4747810022267269 0.0016877392098113906 70-71 0.0 0.0 0.0 0.5420002068841612 0.0017058869432502227 72-73 0.0 0.0 0.0 0.6119415715574203 0.001832921077322048 74-75 0.0 0.0 0.0 0.6813385042275145 0.0019962506782715377 76-77 0.0 0.0 0.0 0.7588656214782055 0.0020325461451492018 78-79 0.0 0.0 0.0 0.852235710020997 0.002068841612026866 80-81 0.0 0.0 0.0 0.9567122114283537 0.0021051370789045305 82-83 0.0 0.0 0.0 1.0667963624683097 0.0021777280126598587 84-85 0.0 0.0 0.0 1.1816352196692395 0.0022684666798540197 86-87 0.0 0.0 0.0 1.3025898630390558 0.0023592053470481806 88-89 0.0 0.0 0.0 1.4355220104785014 0.002431796280803509 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3015 0.0 42.753956 1 GTATCAA 5220 0.0 41.64281 1 TCAACGC 6510 0.0 33.008644 4 ATCAACG 6575 0.0 32.82602 3 CAACGCA 6905 0.0 31.189087 5 AACGCAG 6950 0.0 31.123652 6 TATCAAC 7005 0.0 30.952053 2 GTACATG 9320 0.0 28.120953 1 TACATGG 9580 0.0 26.445827 2 ACGCAGA 8305 0.0 25.760088 7 ACATGGG 9540 0.0 25.55744 3 CGCAGAG 8620 0.0 24.818743 8 CATGGGG 7445 0.0 22.364174 4 TACCTGG 2465 0.0 21.36424 2 GTACCTG 2605 0.0 21.18091 1 GCAGAGT 10420 0.0 20.25829 9 GAGTACA 6985 0.0 20.15671 1 AGAGTAC 8665 0.0 18.749884 10-11 TAAGGTG 875 0.0 18.432377 5 AGTACAT 6880 0.0 18.412138 2 >>END_MODULE