##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936360_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1760149 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.183116883854716 33.0 31.0 34.0 30.0 34.0 2 32.29833269797046 34.0 31.0 34.0 30.0 34.0 3 32.355503426130404 34.0 31.0 34.0 30.0 34.0 4 35.84599428798357 37.0 35.0 37.0 35.0 37.0 5 35.87368001231714 37.0 35.0 37.0 35.0 37.0 6 35.975679331692945 37.0 37.0 37.0 35.0 37.0 7 35.93401013209677 37.0 36.0 37.0 35.0 37.0 8 35.94755387185971 37.0 37.0 37.0 35.0 37.0 9 37.67191300281965 39.0 38.0 39.0 35.0 39.0 10-11 37.624303397041956 39.0 38.0 39.0 35.0 39.0 12-13 37.599955458316316 39.0 38.0 39.0 35.0 39.0 14-15 38.91738625536816 40.5 38.5 41.0 36.0 41.0 16-17 38.886301386984854 40.0 38.5 41.0 36.0 41.0 18-19 38.94056412269643 40.0 39.0 41.0 36.0 41.0 20-21 38.89475379641155 40.0 39.0 41.0 36.0 41.0 22-23 38.762468972797194 40.0 38.5 41.0 35.0 41.0 24-25 38.91967100512514 40.5 39.0 41.0 36.0 41.0 26-27 38.884066917062135 41.0 39.0 41.0 35.0 41.0 28-29 38.8094595968864 40.0 39.0 41.0 35.0 41.0 30-31 38.690400074084636 40.0 38.0 41.0 35.0 41.0 32-33 38.5413774629307 40.0 38.0 41.0 35.0 41.0 34-35 38.31874119747817 40.0 38.0 41.0 34.0 41.0 36-37 38.132943290596415 40.0 38.0 41.0 34.0 41.0 38-39 38.0294284745212 40.0 38.0 41.0 34.0 41.0 40-41 37.9465156074855 40.0 38.0 41.0 34.0 41.0 42-43 37.80853950432605 40.0 38.0 41.0 33.0 41.0 44-45 37.64801644633494 40.0 38.0 41.0 33.0 41.0 46-47 37.418938112625696 40.0 38.0 41.0 33.0 41.0 48-49 37.27814775908176 40.0 38.0 41.0 32.5 41.0 50-51 36.99459591205063 39.5 37.5 40.5 32.5 41.0 52-53 37.10498628241132 40.0 38.0 41.0 32.5 41.0 54-55 37.29266613224222 40.0 38.0 41.0 33.0 41.0 56-57 37.22699953242595 40.0 38.0 41.0 32.0 41.0 58-59 37.10890186001299 40.0 38.0 41.0 32.0 41.0 60-61 36.93254008609499 40.0 37.0 41.0 32.0 41.0 62-63 36.69281833526593 39.5 37.0 41.0 31.0 41.0 64-65 36.450255347700676 39.0 36.5 41.0 31.0 41.0 66-67 36.14481103588389 39.0 36.0 41.0 31.0 41.0 68-69 35.757967649329686 38.5 35.5 40.0 30.0 41.0 70-71 35.38266334270564 37.5 35.0 40.0 30.0 41.0 72-73 34.95595912618761 37.0 35.0 39.0 29.0 41.0 74-75 34.48775813865758 36.5 35.0 39.0 29.0 41.0 76-77 34.069888685560144 36.0 34.0 38.5 29.0 40.0 78-79 33.639231110548025 36.0 34.0 37.5 28.5 39.5 80-81 33.185864094460186 35.0 34.0 37.0 27.5 39.0 82-83 32.78462419942857 35.0 34.0 36.5 26.5 39.0 84-85 32.39151515013786 35.0 33.5 36.0 26.0 37.0 86-87 32.10852916429234 35.0 33.0 36.0 26.0 37.0 88-89 31.779507587141772 35.0 33.0 35.5 25.5 36.5 90-91 31.458668271833805 35.0 33.0 35.0 25.0 36.0 92-93 31.20476675554172 35.0 33.0 35.0 23.5 36.0 94-95 30.894368033615336 35.0 32.0 35.0 20.5 36.0 96-97 30.57989749731415 35.0 32.0 35.0 18.0 35.5 98-99 30.257438148702185 34.5 32.0 35.0 2.0 35.0 100-101 27.098633411148715 31.5 26.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2710142941538862 1101 2 0.1861722168608253 1101 3 0.20475034196950048 1101 4 0.18248309125740292 1101 5 0.3142920045335984 1101 6 0.16688655815763553 1101 7 0.2271547312924369 1101 8 0.15843446293514774 1101 9 0.2547719858612325 1101 10-11 0.4142948321836357 1101 12-13 0.38277459248413237 1101 14-15 0.5026148249243008 1101 16-17 0.47226567954658094 1101 18-19 0.4015393164898029 1101 20-21 0.5053272879046986 1101 22-23 0.49316500982364175 1101 24-25 0.3312760877961125 1101 26-27 0.3042932710347088 1101 28-29 0.36882152564077586 1101 30-31 0.3400452977297519 1101 32-33 0.3671016428424494 1101 34-35 0.24368912778985674 1101 36-37 0.16294964534274925 1101 38-39 0.20971104909454397 1101 40-41 0.25370591301270906 1101 42-43 0.24101962812778766 1101 44-45 0.1702784033111513 1101 46-47 0.17086211568940968 1101 48-49 0.30680544871676574 1101 50-51 0.368661035446074 1101 52-53 0.46782614296853353 1101 54-55 0.4026886914693364 1101 56-57 0.44834128491296354 1101 58-59 0.5203164633285056 1101 60-61 0.4908914835665428 1101 62-63 0.4304740140118284 1101 64-65 0.34670291865397473 1101 66-67 0.4743692537775601 1101 68-69 0.5312102417853666 1101 70-71 0.43786788370394447 1101 72-73 0.5223893914079767 1101 74-75 0.3169401284458999 1101 76-77 0.31477579728954197 1101 78-79 0.3797565752671517 1101 80-81 0.3406815168744828 1101 82-83 0.45282990317998895 1101 84-85 0.4307182209952174 1101 86-87 0.4721929951535131 1101 88-89 0.27593948815547265 1101 90-91 0.3276790974475148 1101 92-93 0.26303757364656377 1101 94-95 0.5227340309788424 1101 96-97 0.5102060732314726 1101 98-99 0.5412388059129007 1101 100-101 0.7258338755545317 1102 1 0.11359198610396959 1102 2 0.17186760004575063 1102 3 0.12941269341009587 1102 4 0.10943418138840855 1102 5 0.19750374862400122 1102 6 0.15635180389902814 1102 7 0.08588769985804134 1102 8 0.05871638309154292 1102 9 0.048597755628428274 1102 10-11 0.2330732179076236 1102 12-13 0.2581322079045165 1102 14-15 0.2702139737995992 1102 16-17 0.24893633958544115 1102 18-19 0.14079893336448634 1102 20-21 0.21665118019093654 1102 22-23 0.16022654351697696 1102 24-25 0.26293131076932497 1102 26-27 0.25648157939456695 1102 28-29 0.1413095753254794 1102 30-31 0.1528291549696803 1102 32-33 -0.1089843159840953 1102 34-35 -0.1403302311249064 1102 36-37 -0.14721616900716583 1102 38-39 -0.10867842985978626 1102 40-41 -0.22520371757056523 1102 42-43 -0.2428772475054899 1102 44-45 -0.2534034858519121 1102 46-47 -0.14697156929909028 1102 48-49 -0.1429987568405764 1102 50-51 -0.23546018426091564 1102 52-53 -0.06264567097608165 1102 54-55 -0.08657539559809635 1102 56-57 -0.06532856453093672 1102 58-59 -0.09201799053315085 1102 60-61 -0.11838982864364311 1102 62-63 0.06341456640844712 1102 64-65 -0.14698516917637505 1102 66-67 0.013672678541240657 1102 68-69 -0.010861722357731196 1102 70-71 0.15200948748989163 1102 72-73 0.015628969898600076 1102 74-75 0.04563220964844561 1102 76-77 0.07084929917894556 1102 78-79 0.17136810955651072 1102 80-81 0.18293884501046875 1102 82-83 0.19759530746421916 1102 84-85 0.18541788364466782 1102 86-87 0.198456271625993 1102 88-89 0.05630103734547731 1102 90-91 0.24312838162064665 1102 92-93 0.3108775765074867 1102 94-95 0.33157219808060745 1102 96-97 0.4174138120291673 1102 98-99 0.401372434874979 1102 100-101 0.7018468212327456 1103 1 0.21037248197833236 1103 2 0.16328406207082935 1103 3 0.17688954110256816 1103 4 0.20246980756313349 1103 5 0.1434698137727537 1103 6 0.10640642252412391 1103 7 0.12851019246384965 1103 8 0.2590540773584422 1103 9 0.16740110861305624 1103 10-11 0.27899778430990096 1103 12-13 0.3585427745406591 1103 14-15 0.3107593899707055 1103 16-17 0.3296385668274482 1103 18-19 0.25771273861414556 1103 20-21 0.33745460162710117 1103 22-23 0.40195103747470284 1103 24-25 0.4196104698421479 1103 26-27 0.3551929791430055 1103 28-29 0.29610203881085795 1103 30-31 0.2979984727074694 1103 32-33 0.37883630104949617 1103 34-35 0.2858255303557016 1103 36-37 0.3057012926956162 1103 38-39 0.3201077246752675 1103 40-41 0.38370696172540875 1103 42-43 0.45018319235271065 1103 44-45 0.42980421038296157 1103 46-47 0.4036675969611494 1103 48-49 0.516423455113916 1103 50-51 0.4379175855308475 1103 52-53 0.43833546777233323 1103 54-55 0.4245565361899466 1103 56-57 0.4095782415486937 1103 58-59 0.47516719655346407 1103 60-61 0.4391943168387016 1103 62-63 0.4643780218422151 1103 64-65 0.4278601731241025 1103 66-67 0.5924390106509989 1103 68-69 0.5549351819213442 1103 70-71 0.5438774444681087 1103 72-73 0.4344832337165485 1103 74-75 0.39419529772560935 1103 76-77 0.4324238842627821 1103 78-79 0.45004217469385566 1103 80-81 0.40566019304947787 1103 82-83 0.5384376321926254 1103 84-85 0.5693361924352729 1103 86-87 0.6807950611175428 1103 88-89 0.5204730731798577 1103 90-91 0.5832918952382293 1103 92-93 0.6094011623414417 1103 94-95 0.6094997136034195 1103 96-97 0.7272240586543646 1103 98-99 0.8052172723453523 1103 100-101 1.237671220213958 1104 1 0.0966185943675626 1104 2 0.06686465810014397 1104 3 0.11793365749656459 1104 4 0.02512019951950606 1104 5 0.040537330388296766 1104 6 0.018143012929712654 1104 7 0.021075003728334707 1104 8 0.10467066307759865 1104 9 0.02946148906636381 1104 10-11 0.09532614895713465 1104 12-13 0.0964831929114851 1104 14-15 0.19873695366361943 1104 16-17 0.19106854190413003 1104 18-19 0.07610473226615966 1104 20-21 0.2131848600200854 1104 22-23 0.20116725786495238 1104 24-25 0.20326293341577184 1104 26-27 0.11707149396918481 1104 28-29 0.1675133214028861 1104 30-31 0.20992877796479803 1104 32-33 0.3122110401002871 1104 34-35 0.18183460499783166 1104 36-37 0.09639871941681832 1104 38-39 0.049938746744118134 1104 40-41 0.1468516127990256 1104 42-43 0.11927761869047515 1104 44-45 0.04927368465018134 1104 46-47 0.19996145240593677 1104 48-49 0.04483629807574374 1104 50-51 -0.019921808214895975 1104 52-53 -0.05220312608417288 1104 54-55 0.09457626714597467 1104 56-57 0.14427143982749868 1104 58-59 0.07657592290827608 1104 60-61 0.11359892658649073 1104 62-63 0.16445950190357195 1104 64-65 0.20642013286337146 1104 66-67 0.1927213949812696 1104 68-69 0.18488389741214206 1104 70-71 0.10078966967260783 1104 72-73 0.09234020159316714 1104 74-75 -0.023518494239276322 1104 76-77 0.03614387510293682 1104 78-79 0.03454312263182402 1104 80-81 0.1447875309414286 1104 82-83 0.14610581129964828 1104 84-85 0.004580845639090114 1104 86-87 -0.1477739519618737 1104 88-89 -0.053637835562476255 1104 90-91 0.03563404758996214 1104 92-93 -0.10216341460129996 1104 94-95 -0.17118178989010602 1104 96-97 0.06112921692805173 1104 98-99 0.14820439530036467 1104 100-101 0.4039271581399966 1105 1 0.13711757279735792 1105 2 0.07872450201761438 1105 3 0.17402874726077044 1105 4 0.041763420312648236 1105 5 0.05667939563481639 1105 6 0.07061362185254438 1105 7 0.09206115391589975 1105 8 0.10048473730053331 1105 9 0.08549587076517895 1105 10-11 0.06288773624277866 1105 12-13 0.1938932612394595 1105 14-15 0.1323321690307111 1105 16-17 0.12616931729266412 1105 18-19 0.14533013921953852 1105 20-21 0.07172187937968033 1105 22-23 0.22147159367685276 1105 24-25 0.22045148010655424 1105 26-27 0.15474650066800422 1105 28-29 0.11738374108401928 1105 30-31 0.18060992245836616 1105 32-33 0.021446830226217628 1105 34-35 -0.13767738589177014 1105 36-37 -0.07642751316624441 1105 38-39 0.04594583074732839 1105 40-41 0.05110745752035939 1105 42-43 0.046065157208687424 1105 44-45 -0.004759842948963922 1105 46-47 -0.08444976309514374 1105 48-49 0.032048667448734136 1105 50-51 0.005332328307083856 1105 52-53 -0.05185625275888128 1105 54-55 0.09834121389034323 1105 56-57 0.215394078493091 1105 58-59 0.2166995580234925 1105 60-61 0.15550842551467525 1105 62-63 0.12565406915972943 1105 64-65 0.24921617888426795 1105 66-67 0.04969675169348875 1105 68-69 0.15211033430924914 1105 70-71 0.07517630887410576 1105 72-73 0.05068818859744795 1105 74-75 0.22778924122427924 1105 76-77 0.16950505041108244 1105 78-79 0.1638575820369681 1105 80-81 0.23161074068151777 1105 82-83 0.10695706397917348 1105 84-85 0.1820501985329841 1105 86-87 0.23629143690468624 1105 88-89 0.1975323647322753 1105 90-91 0.21252144040882825 1105 92-93 0.1758883170439347 1105 94-95 0.24323700365223289 1105 96-97 0.35008432645377496 1105 98-99 0.4748652842339105 1105 100-101 0.5728218108061292 1106 1 -0.1404886660686202 1106 2 -0.0629642736872853 1106 3 -0.07908757272412714 1106 4 -0.10952546464871915 1106 5 -0.02644188507599665 1106 6 -0.09640819529951727 1106 7 -0.028698981931668754 1106 8 -0.12403811919389796 1106 9 -0.04389150971044131 1106 10-11 -0.11479622735713946 1106 12-13 -0.0746525230472912 1106 14-15 -0.07899616073103033 1106 16-17 -0.12171916076867717 1106 18-19 -0.2653279904462096 1106 20-21 -0.09891845594129478 1106 22-23 -0.12672778332024848 1106 24-25 -0.2496996111527281 1106 26-27 -0.2849469385209247 1106 28-29 -0.2538137219163943 1106 30-31 -0.18413096496930592 1106 32-33 -0.2506441659558263 1106 34-35 -0.314965897292403 1106 36-37 -0.3048771758569657 1106 38-39 -0.2453564300984823 1106 40-41 -0.1934732004687163 1106 42-43 -0.13929266778413307 1106 44-45 -0.2184845849209225 1106 46-47 -0.24672856392501075 1106 48-49 -0.10554700728028621 1106 50-51 -0.06301783869063371 1106 52-53 -0.1300965406818193 1106 54-55 -0.09334766223410185 1106 56-57 0.008705533753165184 1106 58-59 -0.03914134527047253 1106 60-61 -0.09705880035500769 1106 62-63 -0.10464070239583378 1106 64-65 -0.24768386951795662 1106 66-67 -0.3046722017941832 1106 68-69 -0.276311010067559 1106 70-71 -0.2501380545362224 1106 72-73 -0.09975938134978435 1106 74-75 -0.18513033677259472 1106 76-77 -0.2581629464799349 1106 78-79 -0.1725820627991581 1106 80-81 -0.09420270790668184 1106 82-83 -0.07286784448840677 1106 84-85 -0.16106513734029448 1106 86-87 -0.06404637447123918 1106 88-89 -0.19234956195743536 1106 90-91 -0.08629983487932336 1106 92-93 -0.11478451404435219 1106 94-95 -0.11939246225633582 1106 96-97 -0.0033818552533944057 1106 98-99 -0.04975578258325086 1106 100-101 -0.08801777699168767 1107 1 -0.11327278380652928 1107 2 -0.062361438709629624 1107 3 0.061970973237905014 1107 4 0.007874530270527202 1107 5 -0.1384470109878606 1107 6 0.019253860654053767 1107 7 -0.0633263069290777 1107 8 -0.018655710120164315 1107 9 -0.02895531545806307 1107 10-11 -0.06211812792848548 1107 12-13 -0.04560696606204573 1107 14-15 0.045263652676418076 1107 16-17 -0.21574528791886394 1107 18-19 -0.09879956586974004 1107 20-21 0.0324001719821041 1107 22-23 0.04474892849923151 1107 24-25 0.09239230283421307 1107 26-27 -0.008574924419022523 1107 28-29 0.05659729863281626 1107 30-31 0.03989783562296623 1107 32-33 0.05383386968119197 1107 34-35 3.7849083815189033E-4 1107 36-37 0.07277796947062143 1107 38-39 0.06584996971054835 1107 40-41 0.09716019562128508 1107 42-43 -0.1695501448532184 1107 44-45 0.06545431866136653 1107 46-47 0.2466172669771609 1107 48-49 0.19715861287561864 1107 50-51 0.22087912854538416 1107 52-53 0.10122394211843755 1107 54-55 0.29982272151169553 1107 56-57 0.2692500046273665 1107 58-59 0.24594153345002212 1107 60-61 0.2838683880670061 1107 62-63 0.3934034106081086 1107 64-65 0.139471067942452 1107 66-67 0.22915430028307782 1107 68-69 0.2426795014308638 1107 70-71 0.3325088063468016 1107 72-73 0.22454789271108666 1107 74-75 0.18684424818862766 1107 76-77 0.26491272967309243 1107 78-79 0.20205668985261127 1107 80-81 0.39712796058690003 1107 82-83 0.34532119891530755 1107 84-85 0.39880948104903524 1107 86-87 0.20458858536058955 1107 88-89 0.36229793280959655 1107 90-91 0.4906739254681618 1107 92-93 0.18599586490698528 1107 94-95 0.1836813106317372 1107 96-97 0.47310052402133707 1107 98-99 0.4153529757016976 1107 100-101 0.22696087781129037 1108 1 -0.12032295655836123 1108 2 -0.10175513356652743 1108 3 -0.10935222213306162 1108 4 -0.028699244924936806 1108 5 -0.1428394057228104 1108 6 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-1.0231078581998077 2316 50-51 -0.8876464308981795 2316 52-53 -0.9967669015509202 2316 54-55 -0.966595831433338 2316 56-57 -1.0149984778953112 2316 58-59 -1.0359289213101803 2316 60-61 -1.1052000479297277 2316 62-63 -1.0036157144360303 2316 64-65 -0.9394454065376863 2316 66-67 -1.0145885306626425 2316 68-69 -1.003064858209278 2316 70-71 -0.8671964343407552 2316 72-73 -0.8977334092340357 2316 74-75 -0.865655795360162 2316 76-77 -1.0454559200558293 2316 78-79 -1.1508632167998982 2316 80-81 -0.9019120657640727 2316 82-83 -0.9440722430067048 2316 84-85 -1.1801244129463555 2316 86-87 -1.1671239430187015 2316 88-89 -1.1019144649233326 2316 90-91 -1.0819154379646818 2316 92-93 -1.2433691884838254 2316 94-95 -1.30433551450853 2316 96-97 -1.458065865604656 2316 98-99 -1.3978215356413095 2316 100-101 -1.703576429775648 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6627.0 3 1218.0 4 938.0 5 767.0 6 791.0 7 822.0 8 1051.0 9 1247.0 10 1705.0 11 2397.0 12 3902.0 13 6996.0 14 8072.0 15 6512.0 16 8496.0 17 12190.0 18 10134.0 19 4991.0 20 4359.0 21 4330.0 22 4560.0 23 5146.0 24 6123.0 25 7228.0 26 8529.0 27 10387.0 28 12984.0 29 15900.0 30 20283.0 31 26264.0 32 33778.0 33 45645.0 34 64304.0 35 97670.0 36 164259.0 37 363538.0 38 632149.0 39 153841.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.14252501797657 24.40765446344576 14.366869690518891 22.082950828058788 2 13.832717768055003 23.587987953861013 41.62969486902665 20.94959940905733 3 16.119988658659164 30.566163598273345 32.025196413727116 21.28865132934038 4 10.183714238961464 19.218802556340396 44.38846010490814 26.209023099790002 5 9.562838890912282 41.71107559280874 38.34527767250446 10.380807843774521 6 25.084331859289517 43.8927370340299 19.512321229810496 11.510609876870086 7 24.746225916603695 34.78514570774362 26.01040891348443 14.458219462168259 8 23.334388294139742 37.420558326169264 22.835734647621308 16.409318732069693 9 25.574405989710296 17.55550858100099 25.170955178319577 31.699130250969134 10-11 21.393483976973933 29.23355710951473 31.676014662869896 17.696944250641444 12-13 22.226695216809393 28.269470997522934 30.62860789076019 18.875225894907484 14-15 21.273681632718 29.42312358057934 28.1446577509388 21.15853703576386 16-17 18.166692096364635 32.42118853578072 30.133907959268157 19.278211408586486 18-19 18.020051827117555 32.061956162588615 32.18335583001092 17.73463618028291 20-21 18.279779439963864 31.286827475121658 32.20963430327857 18.223758781635905 22-23 19.047501348065747 30.53552160342832 31.624940551579066 18.792036496926865 24-25 18.895197763470335 31.52474824408042 31.77510476984447 17.804949222604773 26-27 18.278378839837693 31.23825097522055 32.61235242429416 17.871017760647597 28-29 18.05908544480298 29.81517223665432 33.46655123514475 18.65919108339795 30-31 18.437939853973454 30.708931667326773 32.640183610000356 18.212944868699417 32-33 18.40869676885592 31.27079696705215 32.293996381555026 18.026509882536914 34-35 18.89623099166244 30.63732534890339 32.552893477536955 17.913550181897218 36-37 19.16873587263778 29.83441974973123 31.762820750714972 19.234023626916024 38-39 18.05284522674462 31.88829195046616 31.84846962370042 18.2103931990888 40-41 17.947993894399143 30.768014006563575 33.436758892152085 17.84723320688519 42-43 18.20536370158618 30.720619820235456 31.827225509058316 19.246790969120045 44-45 18.037533818270074 31.010986030109862 32.08166353765874 18.869816613961323 46-47 19.066638187588413 30.71634209845499 31.659292755596397 18.5577269583602 48-49 18.44567729502923 31.181121565305848 31.36423100460886 19.008970135056064 50-51 18.20353418935356 30.599072313136727 31.484347533284573 19.713045964225138 52-53 18.205678917366956 31.677622589104505 31.458846825266956 18.65785166826158 54-55 18.815004284482807 31.227269469555047 31.106572976263596 18.851153269698557 56-57 18.71027646722241 31.5927502018655 30.66168484312738 19.03528848778471 58-59 18.47476326192884 30.47906660415704 31.83679506074988 19.209375073164242 60-61 18.996233620589237 32.115664849333406 30.55535252991519 18.332749000162167 62-63 18.655714817971194 31.36045885847819 31.725501448207833 18.25832487534278 64-65 18.5218805628005 30.815537606744375 31.43377408369593 19.22880774675919 66-67 18.468911076956104 31.40879318889267 30.435594338997834 19.68670139515339 68-69 18.181932400698106 30.981985398258388 31.19783052659472 19.638251674448778 70-71 18.9664268886374 30.51188745544845 31.918523052607657 18.60316260330649 72-73 18.957862881984607 30.486103953318853 31.118441959354143 19.437591205342393 74-75 18.486637631199308 31.328464010069894 30.5204795992146 19.6644187595162 76-77 18.305772995246656 31.024510218206153 31.836273432663997 18.83344335388319 78-79 18.07938720929602 30.70389101425507 31.606928157610646 19.609793618838268 80-81 18.505627566696635 31.21148795096269 30.48131334920665 19.801571133134015 82-83 19.2890290642013 30.847610457299112 30.84892388703248 19.014436591467103 84-85 18.085370651989557 30.672564997084717 31.07191010080994 20.170154250115782 86-87 18.120342186866466 30.77057718335437 31.657396250335506 19.45168437944366 88-89 19.069681504688944 30.362244902330183 31.176695517980026 19.39137807500085 90-91 18.477184126577882 29.91736972477903 32.25411437529135 19.35133177335174 92-93 19.1665072016314 30.89598405159241 31.517669201922722 18.41983954485347 94-95 18.836000529936864 29.45461045072044 31.82890462963809 19.880484389704606 96-97 19.363977628920157 29.418632233342933 31.205133028456533 20.01225710928038 98-99 18.46682973749782 29.764498699904955 31.34601936870467 20.422652193892553 100-101 18.524211595990046 29.875345390605375 31.300267178187298 20.30017583521728 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 580.0 1 383.5 2 375.0 3 587.0 4 506.0 5 486.5 6 919.0 7 1670.5 8 2416.5 9 2538.0 10 2101.5 11 1905.0 12 2115.0 13 2222.0 14 2142.5 15 2125.0 16 2067.5 17 1920.0 18 1801.0 19 1811.0 20 1986.5 21 2389.5 22 2878.5 23 3441.0 24 3986.5 25 5710.0 26 11226.5 27 17535.5 28 17980.0 29 19930.0 30 30019.5 31 39889.0 32 55718.0 33 76667.5 34 94120.0 35 109283.5 36 130089.5 37 141859.0 38 129238.0 39 117113.0 40 104429.0 41 91047.0 42 79041.5 43 72690.0 44 70189.0 45 65099.5 46 55581.0 47 40697.0 48 38458.5 49 33855.5 50 22080.0 51 13036.0 52 7313.5 53 5366.5 54 3970.5 55 3131.5 56 2450.5 57 2063.0 58 1824.5 59 1510.5 60 1173.5 61 894.0 62 647.0 63 442.0 64 265.0 65 187.5 66 158.0 67 133.0 68 108.5 69 92.5 70 97.5 71 77.5 72 54.0 73 61.0 74 56.5 75 44.5 76 30.0 77 23.0 78 27.5 79 28.0 80 27.0 81 26.0 82 25.0 83 29.5 84 27.0 85 17.0 86 11.5 87 10.5 88 9.0 89 6.0 90 6.0 91 5.0 92 6.5 93 9.0 94 7.0 95 9.5 96 15.5 97 11.0 98 4.0 99 5.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05192742205347388 2 0.014146529640388398 3 0.012271688362746565 4 0.007556178482617096 5 0.010226406968955468 6 0.00784024534286586 7 0.00676079127392056 8 0.011930808130448048 9 0.018805226148468114 10-11 0.003238362206835899 12-13 0.011618334584174408 14-15 0.01068091394535349 16-17 0.022469688645677156 18-19 0.014743070046910802 20-21 0.004772323252179219 22-23 0.012498941850945574 24-25 0.02604893108481157 26-27 0.009232172958084798 28-29 0.021333421204682106 30-31 0.018862039520517866 32-33 0.01573730405778147 34-35 0.2783571163577629 36-37 0.4926855624154546 38-39 0.4896744536968177 40-41 0.5096159472862809 42-43 0.5000997074679473 44-45 0.5148143708288333 46-47 0.5201832344875349 48-49 0.5274269394238783 50-51 0.536914772556187 52-53 0.5266315522151818 54-55 0.514871184200883 56-57 0.5103545211229277 58-59 0.5085933065893854 60-61 0.5036505432210568 62-63 0.5025994958381365 64-65 0.5181379530937438 66-67 0.4918333618347083 68-69 0.5184220199539925 70-71 0.5003553676421713 72-73 0.508451273159261 74-75 0.5219444490210773 76-77 0.5091046269378331 78-79 0.5083376464151614 80-81 0.5108942481574003 82-83 0.5118032621101964 84-85 0.5131383763533656 86-87 0.5146723373987089 88-89 0.5269724324474803 90-91 0.55114652225465 92-93 0.5399542879608488 94-95 0.5111499083316242 96-97 0.483623829573519 98-99 0.4746189101036333 100-101 0.48342498277134494 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1760149.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 11.048767586992192 #Duplication Level Percentage of deduplicated Percentage of total 1 70.79127898992458 7.821563887456001 2 10.94215707217348 2.4179470078161547 3 4.436355994718258 1.470487989564048 4 2.3285791804031537 1.0291172068873284 5 1.5392194704651208 0.8503239097271156 6 1.0316513719977212 0.6839085744002665 7 0.7830192090935894 0.6055978080197859 8 0.6198588652929156 0.54789412314865 9 0.5034005352743717 0.5005759965672597 >10 4.538666020079885 10.729878160941984 >50 0.9270966527917088 7.294963883569247 >100 1.2302948362967878 30.022693395751755 >500 0.23061593584140622 17.48176197102456 >1k 0.09729109793309325 18.173879788453764 >5k 5.147677139317104E-4 0.36940629667209285 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6495 0.36900285146314316 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4407 0.2503765306232597 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 4056 0.23043503703379659 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3988 0.22657172773441336 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 3943 0.22401512599217452 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 3849 0.21867466901949778 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 3762 0.21373190565116928 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 3651 0.20742562135364676 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 3397 0.1929950248530096 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 3290 0.18691599404368608 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 3243 0.1842457655573477 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3223 0.18310949811635263 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 3172 0.18021201614181526 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 3072 0.17453067893684002 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 3031 0.17220133068280014 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 2929 0.16640636673372539 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 2748 0.15612314639272018 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 2725 0.15481643883557583 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 2692 0.15294159755793402 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 2628 0.14930554174674987 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 2588 0.14703300686475976 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 2573 0.14618080628401345 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 2541 0.14436277837842137 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 2531 0.14379464465792385 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 2500 0.14203343012438152 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 2466 0.14010177547468994 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 2460 0.1397608952423914 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 2453 0.13936320163804314 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 2392 0.13589758594300824 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 2387 0.13561351908275948 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 2328 0.13226153013182407 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 2317 0.13163658303927678 No Hit ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT 2290 0.13010262199393346 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 2276 0.12930723478523692 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 2270 0.12896635455293842 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 2260 0.12839822083244087 No Hit GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG 2239 0.1272051400193961 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 2209 0.1255007388579035 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 2202 0.12510304525355523 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 2174 0.12351227083616217 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 2111 0.11993302839702775 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 2108 0.11976258828087849 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 2107 0.11970577490882874 No Hit CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG 2075 0.11788774700323666 No Hit CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA 2055 0.11675147956224159 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 2055 0.11675147956224159 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 2047 0.1162969725858436 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 2044 0.11612653246969433 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 2026 0.11510389177279878 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 2011 0.11425169119205249 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 1993 0.11322905049515694 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1897 0.1077749667783807 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 1836 0.1043093510833458 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 1826 0.10374121736284826 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 1818 0.10328671038645024 No Hit GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT 1814 0.10305945689825123 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 1805 0.10254813654980346 No Hit CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT 1799 0.10220725631750494 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 1763 0.10016197492371386 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 5.6813372049752605E-5 0.0 7 0.0 0.0 0.0 5.6813372049752605E-5 0.0 8 0.0 0.0 0.0 5.6813372049752605E-5 0.0 9 0.0 0.0 0.0 1.1362674409950521E-4 0.0 10-11 0.0 0.0 0.0 1.1362674409950521E-4 0.0 12-13 0.0 0.0 0.0 1.1362674409950521E-4 0.0 14-15 0.0 0.0 0.0 1.4203343012438152E-4 0.0 16-17 0.0 0.0 0.0 3.976936043482682E-4 0.0 18-19 0.0 0.0 0.0 4.8291366242289715E-4 0.0 20-21 0.0 0.0 0.0 9.090139527960417E-4 0.0 22-23 0.0 0.0 0.0 0.0016475877894428256 0.0 24-25 0.0 0.0 0.0 0.0027554485444130014 0.0 26-27 0.0 0.0 0.0 0.0040337494155324345 0.0 28-29 0.0 0.0 0.0 0.006419911041622045 0.0 30-31 0.0 0.0 0.0 0.01568049068573172 0.0 32-33 0.0 0.0 0.0 0.03582083107736902 0.0 34-35 0.0 0.0 0.0 0.06391504355597168 0.0 36-37 0.0 0.0 0.0 0.11118376910136585 0.0 38-39 0.0 0.0 0.0 0.1827118045120044 0.0 40-41 0.0 0.0 0.0 0.2584156227682997 0.0 42-43 0.0 0.0 0.0 0.3360226889882618 0.0 44-45 0.0 0.0 0.0 0.4282591985110351 0.0 46-47 0.0 0.0 0.0 0.5397838478446995 0.0 48-49 0.0 0.0 0.0 0.6573023079296128 0.0 50-51 0.0 0.0 0.0 0.7778034700471381 0.0 52-53 0.0 0.0 0.0 0.896316164142922 0.0 54-55 0.0 0.0 0.0 1.0236065242203927 0.0 56-57 0.0 0.0 0.0 1.1800989575314362 0.0 58-59 0.0 0.0 0.0 1.352413914958336 0.0 60-61 0.0 0.0 0.0 1.5296432290675392 0.0 62-63 0.0 0.0 0.0 1.69596437574319 0.0 64-65 0.0 0.0 0.0 1.8842154840300451 0.0 66-67 0.0 0.0 0.0 2.0982314565414635 0.0 68-69 0.0 0.0 0.0 2.335881791825578 0.0 70-71 0.0 0.0 0.0 2.6048647017951323 0.0 72-73 0.0 0.0 0.0 2.8558093661388897 0.0 74-75 0.0 0.0 0.0 3.1230878749469504 0.0 76-77 0.0 0.0 0.0 3.4274655157035 0.0 78-79 0.0 0.0 0.0 3.7758451131125828 0.0 80-81 0.0 0.0 0.0 4.159676254680711 0.0 82-83 0.0 5.6813372049752605E-5 0.0 4.529900593642925 0.0 84-85 0.0 5.6813372049752605E-5 0.0 4.8961479965616554 0.0 86-87 0.0 5.6813372049752605E-5 0.0 5.309976598572053 0.0 88-89 0.0 5.6813372049752605E-5 0.0 5.765023302004546 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCTAG 60 1.528866E-6 47.335423 1 ACACGGG 50 2.8900908E-5 47.32466 3 ATGTATT 155 0.0 45.80847 1 GACATAT 590 0.0 45.73083 1 CATATAG 660 0.0 40.8713 3 GTATGGT 190 0.0 39.86141 1 GGTATGC 215 0.0 39.619522 8 GACGTGC 25 0.0016020297 38.068504 88-89 GGGATAC 65 1.3579163E-4 36.402554 7 ATTGCCG 105 4.8154106E-8 36.055862 8 GGGTATG 435 0.0 35.900448 7 TACAGGG 165 1.8189894E-12 34.41794 2 CATTCCG 605 0.0 34.415005 9 ACATATA 810 0.0 33.886795 2 CATGGGT 1095 0.0 33.709763 4 GTACAGG 170 3.6379788E-12 33.41324 1 TGGGTAT 550 0.0 31.834782 6 AGACGTG 30 0.003917356 31.723753 88-89 GTACGGG 60 0.0040192246 31.556948 1 GTCAAGA 75 3.148195E-4 31.547085 9 >>END_MODULE