##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936360_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1760149 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.549104649663185 34.0 31.0 34.0 31.0 34.0 2 32.69910558708382 34.0 31.0 34.0 31.0 34.0 3 32.783018369467584 34.0 31.0 34.0 31.0 34.0 4 36.25013393752461 37.0 35.0 37.0 35.0 37.0 5 36.23047310199307 37.0 37.0 37.0 35.0 37.0 6 36.268084690557444 37.0 37.0 37.0 35.0 37.0 7 36.22838066550048 37.0 37.0 37.0 35.0 37.0 8 36.21414039379621 37.0 37.0 37.0 35.0 37.0 9 37.98475469974417 39.0 38.0 39.0 35.0 39.0 10-11 37.933799638553324 39.0 38.0 39.0 35.0 39.0 12-13 37.92540375843181 39.0 38.0 39.0 35.0 39.0 14-15 39.2764373925162 41.0 39.0 41.0 36.0 41.0 16-17 39.20910019549481 41.0 39.0 41.0 36.0 41.0 18-19 39.31834890114416 41.0 39.0 41.0 36.0 41.0 20-21 39.293924832499975 41.0 39.0 41.0 36.0 41.0 22-23 39.18228286355303 40.0 39.0 41.0 36.0 41.0 24-25 39.32154948245859 41.0 39.0 41.0 36.0 41.0 26-27 39.24147330708934 41.0 39.0 41.0 36.0 41.0 28-29 39.19191329824918 41.0 39.0 41.0 36.0 41.0 30-31 39.092116065174025 40.5 39.0 41.0 35.0 41.0 32-33 38.95284007206208 40.0 38.0 41.0 35.0 41.0 34-35 38.83851026248346 40.0 38.0 41.0 35.0 41.0 36-37 38.6930779723762 40.0 38.0 41.0 35.0 41.0 38-39 38.599981876534315 40.0 38.0 41.0 35.0 41.0 40-41 38.535807764001795 40.0 38.0 41.0 35.0 41.0 42-43 38.549784137592894 40.0 38.0 41.0 35.0 41.0 44-45 38.42957300773969 40.0 38.0 41.0 35.0 41.0 46-47 38.24701573559966 40.0 38.0 41.0 34.0 41.0 48-49 38.053222482869344 40.0 38.0 41.0 34.0 41.0 50-51 37.94108112438208 40.0 38.0 41.0 33.5 41.0 52-53 37.85900937931959 40.0 38.0 41.0 33.0 41.0 54-55 37.763435368255756 40.0 38.0 41.0 33.0 41.0 56-57 37.61243565175448 40.0 38.0 41.0 33.0 41.0 58-59 37.45729168382904 40.0 38.0 41.0 33.0 41.0 60-61 37.28748816151359 40.0 37.0 41.0 32.0 41.0 62-63 37.05882002035055 39.5 37.0 41.0 32.0 41.0 64-65 36.76198776353593 39.0 36.5 41.0 31.5 41.0 66-67 36.4611015885587 39.0 36.0 40.5 31.0 41.0 68-69 36.09166780766856 38.5 35.5 40.0 31.0 41.0 70-71 35.74101283470888 37.5 35.0 40.0 31.0 41.0 72-73 35.31675187725585 37.0 35.0 39.0 30.0 41.0 74-75 34.832315332395154 36.5 35.0 39.0 30.0 40.5 76-77 34.02079681890567 36.0 34.0 38.0 29.0 39.0 78-79 33.988195601622365 36.0 34.0 37.0 29.0 39.0 80-81 33.74287460891095 35.0 34.0 37.0 29.0 39.0 82-83 33.39588239404732 35.0 34.0 37.0 29.0 39.0 84-85 33.02199586512278 35.0 34.0 36.0 29.0 37.5 86-87 32.653177373051946 35.0 34.0 36.0 28.5 37.0 88-89 32.133258036677574 35.0 33.0 35.5 26.0 36.5 90-91 31.91811204619609 35.0 33.0 35.0 26.0 36.0 92-93 31.672491078880256 35.0 33.0 35.0 25.5 36.0 94-95 31.538239660392385 35.0 33.0 35.0 25.0 36.0 96-97 31.36278718449404 35.0 33.0 35.0 25.0 35.5 98-99 31.0685598207879 35.0 33.0 35.0 24.5 35.0 100-101 27.978310643019427 32.0 27.5 34.5 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06722353554173566 1101 2 0.21493106114749594 1101 3 0.23604148823088877 1101 4 0.16130112979030997 1101 5 0.14463585605264484 1101 6 0.2246785051050253 1101 7 0.14860752803620159 1101 8 0.19706607334227755 1101 9 0.1920331780524691 1101 10-11 0.1908658488702315 1101 12-13 0.347236500331185 1101 14-15 0.4097861484615848 1101 16-17 0.31247861527303655 1101 18-19 0.28172957728781967 1101 20-21 0.33773331639115867 1101 22-23 0.3230681229894685 1101 24-25 0.3012816176313038 1101 26-27 0.2875095015667455 1101 28-29 0.3468356598306386 1101 30-31 0.33557496750497506 1101 32-33 0.39111519305816245 1101 34-35 0.43668669052448905 1101 36-37 0.3829788676170196 1101 38-39 0.34880281826557535 1101 40-41 0.29522639546452467 1101 42-43 0.22312238230368564 1101 44-45 0.3238626998497409 1101 46-47 0.2836946197365151 1101 48-49 0.1366590352698509 1101 50-51 0.09992466870821914 1101 52-53 0.22164987278134873 1101 54-55 0.10963294458725414 1101 56-57 0.1096099448511012 1101 58-59 -0.04548536442346318 1101 60-61 0.09229425377115064 1101 62-63 0.15697070874743702 1101 64-65 0.16072785349809493 1101 66-67 0.06849486285516804 1101 68-69 0.26489582861589867 1101 70-71 0.1879435883126348 1101 72-73 0.3043908242375366 1101 74-75 0.2116647970582406 1101 76-77 0.1199388788029836 1101 78-79 0.10802125092822479 1101 80-81 0.25930118530155255 1101 82-83 0.2593761399100458 1101 84-85 0.28433727248468443 1101 86-87 0.23365481995023885 1101 88-89 0.16325621249273325 1101 90-91 0.4286256549661509 1101 92-93 0.57688792895269 1101 94-95 0.45827287141868567 1101 96-97 0.40195612299517336 1101 98-99 0.651464639124093 1101 100-101 0.7911324660559096 1102 1 0.0022090521172088984 1102 2 0.12208515763213512 1102 3 0.020971574416208227 1102 4 0.10915484124235064 1102 5 0.08207699851470807 1102 6 0.07954028674505054 1102 7 0.0985413691834367 1102 8 0.08692052386618343 1102 9 0.14520343875410902 1102 10-11 0.013658467687953646 1102 12-13 0.17911851108349452 1102 14-15 0.1597280359557729 1102 16-17 0.21500606225654906 1102 18-19 0.2886673164431386 1102 20-21 0.18973119453966092 1102 22-23 0.21047886814080385 1102 24-25 0.10437707710751454 1102 26-27 0.13266947486479808 1102 28-29 0.1189511934524532 1102 30-31 0.09150542322621646 1102 32-33 0.15807659443378697 1102 34-35 0.04926551492340536 1102 36-37 0.03875912486171984 1102 38-39 0.1399837239862336 1102 40-41 -0.04322828870145656 1102 42-43 0.1310453519421344 1102 44-45 0.1179849924029952 1102 46-47 0.2235302954508498 1102 48-49 0.13561450022950083 1102 50-51 0.012621804208698961 1102 52-53 -0.0054746608285327625 1102 54-55 0.00803589372242186 1102 56-57 0.1868965974515575 1102 58-59 0.05578983256302905 1102 60-61 0.043041669999745125 1102 62-63 0.07417826873742683 1102 64-65 -0.022286308072558825 1102 66-67 0.07354767073224622 1102 68-69 0.060728714929958016 1102 70-71 0.10390098439555118 1102 72-73 0.007862077917998533 1102 74-75 0.010471851618426342 1102 76-77 -0.04770676007696295 1102 78-79 -0.09291540346030303 1102 80-81 -0.09387235354143542 1102 82-83 0.061431534538655796 1102 84-85 0.004099398627559481 1102 86-87 0.06345074075034773 1102 88-89 0.14674332045682092 1102 90-91 0.2910361021046235 1102 92-93 0.21984171311986955 1102 94-95 0.28936961831041685 1102 96-97 0.1570685334423132 1102 98-99 0.10490162132676417 1102 100-101 0.5357056520516927 1103 1 -0.0032499582446448017 1103 2 0.05876463044118907 1103 3 0.105870023703595 1103 4 0.021673772371542555 1103 5 0.05698095244682122 1103 6 0.08509484404896739 1103 7 0.13990321258341964 1103 8 0.09945713831002934 1103 9 0.1627740935786619 1103 10-11 0.16960319877322405 1103 12-13 0.2203990682790291 1103 14-15 0.29432943407848455 1103 16-17 0.19776910869235564 1103 18-19 0.15524171613738957 1103 20-21 0.12642207593881238 1103 22-23 0.27770255921900144 1103 24-25 0.2166133604170284 1103 26-27 0.26447933359792586 1103 28-29 0.32652122080454404 1103 30-31 0.3366717462091131 1103 32-33 0.2145491154184569 1103 34-35 0.3337009180601953 1103 36-37 0.35020222594467754 1103 38-39 0.2742371601109639 1103 40-41 0.2810665891268087 1103 42-43 0.17644155801550454 1103 44-45 0.3147760412232046 1103 46-47 0.3390600961142596 1103 48-49 0.2888774996048795 1103 50-51 0.31761120848064905 1103 52-53 0.229309845165254 1103 54-55 0.22715212729048773 1103 56-57 0.34334615864866436 1103 58-59 0.3206224257640784 1103 60-61 0.3594931421356833 1103 62-63 0.46663590721632175 1103 64-65 0.45331214378177265 1103 66-67 0.3876946076684078 1103 68-69 0.3468461720693128 1103 70-71 0.15117746872443405 1103 72-73 0.22980550271970657 1103 74-75 0.288847867339463 1103 76-77 0.2703832708117915 1103 78-79 0.17506239542336033 1103 80-81 0.17545878934259918 1103 82-83 0.3451018491020079 1103 84-85 0.2777194583515339 1103 86-87 0.2698834742770586 1103 88-89 0.40775047079067406 1103 90-91 0.5508225332979997 1103 92-93 0.6328804481353991 1103 94-95 0.6538861935657536 1103 96-97 0.6474881699075894 1103 98-99 0.5963045356511039 1103 100-101 0.9280156918961922 1104 1 -2.343971505709419E-4 1104 2 0.03470937311330857 1104 3 0.006090232889008007 1104 4 -0.004208129468949551 1104 5 -0.011302534260742902 1104 6 -0.05693874916848074 1104 7 -0.14559653179285448 1104 8 -0.11346410828165432 1104 9 -0.06908461040907099 1104 10-11 -0.03166520080925039 1104 12-13 0.018162760124631916 1104 14-15 0.017425037350470518 1104 16-17 0.006806206085002486 1104 18-19 0.12741600891175153 1104 20-21 0.11538032938972975 1104 22-23 0.15842413705642144 1104 24-25 0.0581898930942657 1104 26-27 0.16675518354680463 1104 28-29 0.20910139611981293 1104 30-31 -0.011483103185909727 1104 32-33 -0.08797357118685767 1104 34-35 -0.011928315707699255 1104 36-37 -0.06909723192001138 1104 38-39 0.02310948671287605 1104 40-41 0.12125915899729023 1104 42-43 0.16207470602163454 1104 44-45 0.11656713806199548 1104 46-47 0.13915502304848104 1104 48-49 0.04597505200774066 1104 50-51 0.06291545396462794 1104 52-53 -0.005085125438021976 1104 54-55 -0.0242451492517759 1104 56-57 -0.02824951918023544 1104 58-59 0.04071644736428226 1104 60-61 -0.1352865421690197 1104 62-63 -0.11515636123119322 1104 64-65 -0.21254438207882487 1104 66-67 -0.18796269145395428 1104 68-69 -0.1254119972388068 1104 70-71 -0.11457933725716885 1104 72-73 -0.20197491650319677 1104 74-75 -0.2258658074645581 1104 76-77 -0.3189132846585494 1104 78-79 -0.3213602038438452 1104 80-81 -0.19850074222692626 1104 82-83 -0.10784474684209044 1104 84-85 -0.03720497288569646 1104 86-87 -0.039535061638083846 1104 88-89 0.01508580650983049 1104 90-91 0.15796481985219302 1104 92-93 0.14399808297691052 1104 94-95 0.08599945617652338 1104 96-97 0.14379027986839077 1104 98-99 0.05797895091183847 1104 100-101 0.09475158998753486 1105 1 0.03905963085049535 1105 2 0.10583577986572124 1105 3 0.1543062531194508 1105 4 0.12444272798013856 1105 5 -0.009021585707685631 1105 6 0.1294082162656025 1105 7 0.08020397731753803 1105 8 0.08055468191127346 1105 9 0.13674420122008257 1105 10-11 0.07721262106909421 1105 12-13 0.15771322736739535 1105 14-15 0.18878757754340114 1105 16-17 0.21797439673343888 1105 18-19 0.23002017557842436 1105 20-21 0.17545054140741456 1105 22-23 0.08717526748247195 1105 24-25 0.14246001467786584 1105 26-27 0.15357315983298037 1105 28-29 0.1426505570924803 1105 30-31 0.15136586352094383 1105 32-33 0.2179473492311459 1105 34-35 0.2697229855382801 1105 36-37 0.2773994940312221 1105 38-39 0.19688569585006377 1105 40-41 0.16655658665001738 1105 42-43 0.23598824098947802 1105 44-45 0.2525224318052892 1105 46-47 0.28372416325479577 1105 48-49 0.23973133215243791 1105 50-51 0.22014892462379265 1105 52-53 0.2678838757677653 1105 54-55 0.3235511479749178 1105 56-57 0.41629136037495584 1105 58-59 0.3491668007270974 1105 60-61 0.3842158191653837 1105 62-63 0.3258586357543365 1105 64-65 0.4548267074844077 1105 66-67 0.2972109336130373 1105 68-69 0.3418814278683797 1105 70-71 0.3226363815263298 1105 72-73 0.21772296666936342 1105 74-75 0.2713836984973952 1105 76-77 0.24425869082377005 1105 78-79 0.24638957844447162 1105 80-81 0.2758375398604187 1105 82-83 0.2857804876166341 1105 84-85 0.25788437263949504 1105 86-87 0.2952203057922489 1105 88-89 0.4171339710328681 1105 90-91 0.5470218770601143 1105 92-93 0.4863381904929902 1105 94-95 0.3649760437970997 1105 96-97 0.3584408107499577 1105 98-99 0.35876417833681273 1105 100-101 0.6282473697302997 1106 1 -0.17217812047453407 1106 2 -0.13909313313278915 1106 3 -0.17698829550469952 1106 4 -0.11534309719745295 1106 5 -0.16635211264924976 1106 6 -0.12094415594549446 1106 7 -0.02219384076641262 1106 8 -0.046550362966229386 1106 9 -0.008722759742816777 1106 10-11 0.08199158759122582 1106 12-13 -0.0344831691773706 1106 14-15 -0.04275219562891408 1106 16-17 -0.18873523611258491 1106 18-19 -0.026444533978107643 1106 20-21 -0.036330881376976265 1106 22-23 -0.02424062812939809 1106 24-25 0.030562506890333907 1106 26-27 -0.11779963570678831 1106 28-29 -0.05948135001541033 1106 30-31 -0.01633338393492778 1106 32-33 0.05527430123662924 1106 34-35 0.049872048322590956 1106 36-37 0.017761526603301547 1106 38-39 0.049833913503420035 1106 40-41 0.10177914650509479 1106 42-43 0.15669354776117217 1106 44-45 0.12097824017264713 1106 46-47 0.2062783948343352 1106 48-49 0.16791910064921467 1106 50-51 0.16602117890216306 1106 52-53 0.22022937526466535 1106 54-55 0.26481796071855257 1106 56-57 0.32486759187014513 1106 58-59 0.401152999063747 1106 60-61 0.22413359563547886 1106 62-63 0.16588807876724587 1106 64-65 0.30185559417299146 1106 66-67 0.3215847674909824 1106 68-69 0.34134177319113945 1106 70-71 0.24943916354961715 1106 72-73 0.030181418079351374 1106 74-75 0.2291620966448562 1106 76-77 0.19507821479491838 1106 78-79 0.09401535895543844 1106 80-81 0.22979216227285804 1106 82-83 0.28895233961428346 1106 84-85 0.30447804098584896 1106 86-87 0.24253860163226904 1106 88-89 0.3171837791311063 1106 90-91 0.2891966834010127 1106 92-93 0.2400080230534236 1106 94-95 0.16350228643885956 1106 96-97 0.1502435626473826 1106 98-99 -0.12155138006619381 1106 100-101 0.11587608249221759 1107 1 0.026585241191369846 1107 2 0.037387512235319775 1107 3 0.034739848904720816 1107 4 -0.005748101493907143 1107 5 9.331823306055753E-4 1107 6 0.05804218611234546 1107 7 0.01948645185250797 1107 8 0.03206576986553955 1107 9 0.06824777473187993 1107 10-11 0.06445675118934702 1107 12-13 0.05130851444193496 1107 14-15 0.13558594761516218 1107 16-17 0.1081539337856583 1107 18-19 0.023499839877970885 1107 20-21 -0.0013904451018760255 1107 22-23 0.008334235305291315 1107 24-25 0.1586119143579836 1107 26-27 0.18688788004454437 1107 28-29 0.11568616351006966 1107 30-31 0.06466082199431611 1107 32-33 0.049965724326376915 1107 34-35 -0.012129428320669433 1107 36-37 0.023744714160791602 1107 38-39 0.20113218616950945 1107 40-41 0.1787559311083271 1107 42-43 0.07387390106682545 1107 44-45 0.13006774160997736 1107 46-47 0.21268175232274444 1107 48-49 0.08254476432213664 1107 50-51 0.340071330739967 1107 52-53 0.32438272680536073 1107 54-55 0.2291170249331813 1107 56-57 0.08664273321377891 1107 58-59 0.16235091079499142 1107 60-61 0.3304890085895309 1107 62-63 0.2694081511558295 1107 64-65 0.16951557427741903 1107 66-67 0.1321516380193728 1107 68-69 0.17300927925678167 1107 70-71 0.3808104135229087 1107 72-73 0.19540175123942305 1107 74-75 0.2654834080911783 1107 76-77 0.16822685615810684 1107 78-79 0.21899050965410538 1107 80-81 0.34897465352570833 1107 82-83 0.42730982196573564 1107 84-85 0.28681497614056184 1107 86-87 0.3547967569411128 1107 88-89 0.4823109652143671 1107 90-91 0.2629489662100575 1107 92-93 0.4489963933787813 1107 94-95 0.5255194085651596 1107 96-97 0.21362006756744734 1107 98-99 0.28258475337848665 1107 100-101 0.4224882345969583 1108 1 -0.11315395733576139 1108 2 -0.0680105342940962 1108 3 -0.014092058777656291 1108 4 -0.001314610950430506 1108 5 0.012424317591111844 1108 6 0.017858704535221648 1108 7 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-0.5874784637571437 2316 76-77 -0.5445447365187306 2316 78-79 -0.6368843802202449 2316 80-81 -0.7116826058989147 2316 82-83 -0.9053468335823567 2316 84-85 -0.7522568645796142 2316 86-87 -0.8564201440004382 2316 88-89 -0.8478643331740123 2316 90-91 -0.8612784386991912 2316 92-93 -0.8635788602467223 2316 94-95 -0.757766513774417 2316 96-97 -0.7768377331290175 2316 98-99 -0.9375209830790254 2316 100-101 -1.1491223368317627 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 2.0 5 11.0 6 28.0 7 70.0 8 155.0 9 367.0 10 666.0 11 1226.0 12 2237.0 13 3519.0 14 4259.0 15 5890.0 16 7806.0 17 11546.0 18 9977.0 19 4724.0 20 3560.0 21 3625.0 22 4047.0 23 4611.0 24 5455.0 25 6851.0 26 8328.0 27 10594.0 28 13803.0 29 17610.0 30 22300.0 31 28607.0 32 35807.0 33 46884.0 34 63254.0 35 93160.0 36 149126.0 37 344527.0 38 676736.0 39 168762.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.487574876056414 24.715166182970474 14.654741191202996 22.142517749770114 2 13.77893602705848 23.712653658713993 41.3593681808263 21.14904213340122 3 15.98841916224138 30.68762928592977 32.091146829046856 21.232804722781992 4 10.135107880071518 19.520051995598102 44.10189137396891 26.242948750361478 5 9.548509813657821 41.767145849584324 38.28545197025934 10.398892366498517 6 24.867951520013364 44.06183794667383 19.44659230553777 11.623618227775035 7 24.451566316260724 34.96675565534509 25.96393828022514 14.617739748169049 8 23.139802369003988 37.55909300860325 22.768583796030903 16.53252082636186 9 25.377567467299645 17.483747114590866 25.108556150644063 32.030129267465426 10-11 21.203816962599063 29.432409753071198 31.578684231906156 17.78508905242358 12-13 22.04712957294772 28.302457606535814 30.662685939672667 18.98772688084379 14-15 21.02935603747183 29.562838146088772 28.114324412308278 21.29348140413113 16-17 17.984186467197457 32.483305286290054 30.10383276765799 19.428675478854498 18-19 17.87144156545838 32.17349781183297 32.05918930726887 17.895871315439773 20-21 18.11838088707263 31.3232572924224 32.238946816434286 18.319415004070677 22-23 18.879878919341486 30.597807344719115 31.600052041048798 18.9222616948906 24-25 18.66688862368939 31.664527110809864 31.731199440031975 17.937384825468772 26-27 18.030953905486104 31.331355416535757 32.67428971405618 17.96340096392196 28-29 17.861518589568227 29.910855180646024 33.47048357961007 18.757142650175684 30-31 18.331192860963608 30.812461952286924 32.58565937485708 18.27068581189239 32-33 18.1976797532206 31.34671068274251 32.27838302564975 18.177226538387135 34-35 18.750717283085073 30.664121755749914 32.61535900515535 17.96980195600967 36-37 18.991453554731507 29.92144974254991 31.809417054371025 19.277679648347554 38-39 17.908054812482938 31.937966527218048 31.8681996072486 18.285779053050412 40-41 17.74180902758248 30.779983853547932 33.47366315955729 18.004543959312304 42-43 18.002737944507622 30.784688987059962 31.838760692343726 19.373812376088694 44-45 17.89722117823588 31.067982401941276 32.09668535047119 18.938111069351656 46-47 18.802734029852953 30.910515582481434 31.63479256977555 18.651957817890064 48-49 18.223349821153086 31.28974336391298 31.40975120533444 19.07715560959949 50-51 18.028740304891315 30.64060585089973 31.464622618729038 19.86603122547992 52-53 18.071664161953166 31.678366377559282 31.492657733030683 18.75731172745687 54-55 18.669260775551063 31.26665348591193 31.130244037128822 18.933841701408184 56-57 18.545365810423544 31.658401947170944 30.655414603327554 19.140817639077955 58-59 18.185363897036975 30.67741702729405 31.80142682885951 19.335792246809465 60-61 18.81577829775303 32.23859194211325 30.47138941434039 18.474240345793326 62-63 18.43224636096149 31.467648477486847 31.738193755187773 18.36191140636389 64-65 18.317262568943693 30.89626232578011 31.411962284646833 19.37451282062936 66-67 18.303564073268795 31.509179052455217 30.363111304781583 19.8241455694944 68-69 18.06520357083406 31.01285743422858 31.12943847367467 19.79250052126269 70-71 18.80927058406452 30.560129034603907 31.864507984555846 18.766092396775726 72-73 18.78071679262783 30.59965655994648 31.001103610097537 19.618523037328156 74-75 18.312682619482782 31.478329391432204 30.41219805823257 19.796789930852444 76-77 18.131991844265666 31.083532515767367 31.65006741887746 19.13440822108951 78-79 17.916224929018636 30.812651290404773 31.413291260192196 19.85783252038439 80-81 18.259067322188105 31.4004882038241 30.253581349738983 20.08686312424881 82-83 19.053472177639506 31.011834793531683 30.587126430773758 19.347566598055053 84-85 17.881642769385913 30.8097236721563 30.72796869562747 20.580664862830318 86-87 17.82842837217028 30.949095195079718 31.455168184724464 19.76730824802554 88-89 18.761216259776006 30.566665447404457 30.850029605210516 19.822088687609018 90-91 18.2268177192848 30.00852869594159 31.954598728132925 19.810054856640686 92-93 18.892285601137345 31.059422837492484 31.15053576567054 18.89775579569963 94-95 18.505155503109325 29.539582622558424 31.463468608061035 20.49179326627122 96-97 19.005088924076343 29.58260254974584 30.8677021777777 20.544606348400116 98-99 18.076996608981187 29.844999447471594 31.031330491244624 21.046673452302596 100-101 18.013370023773792 29.989691111147092 31.057821532084397 20.939117332994723 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 587.0 1 377.5 2 342.0 3 539.5 4 438.0 5 429.5 6 848.5 7 1469.5 8 1999.5 9 2049.0 10 1547.0 11 1145.0 12 1096.5 13 1115.0 14 1104.0 15 1138.0 16 1237.0 17 1351.0 18 1444.0 19 1633.0 20 1960.5 21 2352.5 22 2908.0 23 3582.5 24 4117.5 25 6046.5 26 12130.0 27 18532.5 28 18609.0 29 20595.0 30 31060.0 31 41600.5 32 57058.5 33 77275.5 34 94000.5 35 108727.0 36 129627.5 37 141496.0 38 129426.5 39 117416.0 40 104262.5 41 90562.5 42 78299.5 43 71544.0 44 69850.0 45 65641.0 46 55764.5 47 41015.5 48 39261.5 49 34064.0 50 22305.0 51 13944.0 52 8180.0 53 5850.5 54 4211.0 55 3244.0 56 2430.5 57 2038.0 58 1800.0 59 1448.5 60 1131.0 61 856.0 62 593.0 63 405.0 64 223.0 65 141.5 66 130.0 67 113.5 68 93.0 69 85.0 70 88.0 71 78.5 72 58.5 73 51.5 74 38.5 75 27.0 76 22.5 77 16.5 78 21.0 79 21.5 80 16.5 81 12.5 82 14.0 83 31.5 84 29.5 85 10.5 86 2.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27923772362453403 2 0.023009415680149806 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.4088023229851566E-4 12-13 8.522005807462891E-5 14-15 0.0 16-17 6.249470925472787E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0027554485444130014 26-27 0.0027270418583881253 28-29 0.0020168747077662177 30-31 0.0013351142431691863 32-33 0.001960061335716465 34-35 0.001988468021741341 36-37 1.1362674409950521E-4 38-39 1.4203343012438152E-4 40-41 5.681337204975261E-4 42-43 0.0039485293574578065 44-45 0.0038917159854080534 46-47 0.014544223244736667 48-49 0.014998730221134687 50-51 0.0063630976695722916 52-53 0.00761299185466685 54-55 3.976936043482682E-4 56-57 0.003238362206835899 58-59 0.0012498941850945574 60-61 0.0012783008711194335 62-63 0.0 64-65 9.658273248457943E-4 66-67 0.0 68-69 0.0 70-71 5.6813372049752605E-5 72-73 9.374206388209179E-4 74-75 0.0 76-77 0.20415885246078597 78-79 0.11027475514856981 80-81 0.0013351142431691863 82-83 0.0 84-85 6.53353778572155E-4 86-87 0.1250462318815055 88-89 0.594097431524263 90-91 0.3115929390068682 92-93 0.2945205207059175 94-95 0.0022441281959652277 96-97 0.0029542953465871357 98-99 0.0033235822649105274 100-101 9.942340108706706E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1760149.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 10.168460407639191 #Duplication Level Percentage of deduplicated Percentage of total 1 68.68883370990248 6.984596860260557 2 11.552897266098434 2.3495035688768997 3 4.632529049199244 1.4131706467206284 4 2.5745715626023102 1.0471771600382143 5 1.641644878424773 0.834650047483298 6 1.1423655139070787 0.6969659099529917 7 0.9083538613360902 0.646559219258555 8 0.6963597269180225 0.5664725050112281 9 0.5565388291344086 0.5093228744430396 >10 4.8748963387927615 10.678263701219523 >50 1.0267791265180857 7.46009531858557 >100 1.343183346483137 30.319181042337917 >500 0.2527885566916747 17.6381368432918 >1k 0.10770020185760093 18.520324564675587 >5k 5.580321339772069E-4 0.3355797378441959 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5914 0.33599428230223694 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4247 0.2412863910952993 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 4064 0.23088954401019457 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 3991 0.22674216785056267 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 3973 0.2257195271536671 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3969 0.22549227366546806 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 3798 0.2157771870449604 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 3433 0.1950403062468007 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 3406 0.19350634520145737 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 3362 0.19100655683126827 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 3178 0.18055289637411379 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 3171 0.18015520276976552 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 3130 0.17782585451572566 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3037 0.17254221091509866 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 3013 0.1711786899859046 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 2991 0.16992879580081005 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 2692 0.15294159755793402 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 2644 0.1502145556995459 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 2644 0.1502145556995459 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 2632 0.14953279523494886 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 2592 0.14726026035295875 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 2590 0.14714663360885927 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 2558 0.14532860570326717 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 2530 0.14373783128587408 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 2530 0.14373783128587408 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 2510 0.14260156384487904 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 2487 0.14129485628773475 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 2450 0.1391927615218939 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 2429 0.13799968070884908 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 2420 0.1374883603604013 No Hit ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT 2418 0.13737473361630181 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 2377 0.13504538536226193 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 2376 0.1349885719902122 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 2354 0.13373867780511764 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 2349 0.13345461094486888 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 2339 0.13288647722437136 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 2258 0.1282845940883414 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 2236 0.1270346999032468 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 2226 0.12646656618274932 No Hit GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG 2180 0.12385315106846068 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 2117 0.12027390862932627 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 2099 0.11925126793243072 No Hit CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG 2069 0.11754686677093813 No Hit CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA 2062 0.11714917316658986 No Hit TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT 2024 0.11499026502869929 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 2022 0.11487663828459976 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 2020 0.11476301154050025 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 2005 0.11391081095975399 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1996 0.1133994906113062 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 1979 0.11243366328646041 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 1953 0.11095651561316684 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 1929 0.10959299468397278 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 1922 0.10919530107962451 No Hit CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT 1874 0.10646825922123637 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1872 0.10635463247713688 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 1853 0.10527517840819157 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 1820 0.10340033713054973 No Hit CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG 1816 0.10317308364235073 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 1802 0.1023776964336542 No Hit GTACATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT 1790 0.10169593596905716 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 1787 0.10152549585290789 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.6813372049752605E-5 0.0 10-11 0.0 0.0 0.0 8.52200580746289E-5 0.0 12-13 0.0 0.0 0.0 1.1362674409950521E-4 0.0 14-15 0.0 0.0 0.0 1.4203343012438152E-4 0.0 16-17 0.0 0.0 0.0 3.976936043482682E-4 0.0 18-19 0.0 0.0 0.0 4.8291366242289715E-4 0.0 20-21 0.0 0.0 0.0 8.237938947214128E-4 0.0 22-23 0.0 0.0 0.0 0.0015339610453433204 0.0 24-25 0.0 0.0 0.0 0.0025850084282637437 0.0 26-27 0.0 0.0 0.0 0.0038064959273334245 0.0 28-29 0.0 0.0 0.0 0.006079030809323529 0.0 30-31 0.0 0.0 0.0 0.015510050569582462 0.0 32-33 0.0 0.0 0.0 0.035593577589170006 0.0 34-35 0.0 0.0 0.0 0.06380141681187218 0.0 36-37 0.0 0.0 0.0 0.11163827607776386 0.0 38-39 0.0 0.0 0.0 0.18347878503467602 0.0 40-41 0.0 0.0 0.0 0.2595518902092948 0.0 42-43 0.0 0.0 0.0 0.33755665003360513 0.0 44-45 0.0 0.0 0.0 0.4304749200209755 0.0 46-47 0.0 0.0 0.0 0.5426245164471872 0.0 48-49 0.0 0.0 0.0 0.6610519904848964 0.0 50-51 0.0 0.0 0.0 0.7820076595788197 0.0 52-53 0.0 0.0 0.0 0.901827061231748 0.0 54-55 0.0 0.0 0.0 1.0300832486340645 0.0 56-57 0.0 0.0 0.0 1.1872006290376553 0.0 58-59 0.0 0.0 0.0 1.3605098204754258 0.0 60-61 0.0 0.0 0.0 1.5392446889439473 0.0 62-63 0.0 0.0 0.0 1.706730509746618 0.0 64-65 0.0 0.0 0.0 1.8967144258809907 0.0 66-67 0.0 0.0 0.0 2.1116962257172545 0.0 68-69 0.0 0.0 0.0 2.3498010679777677 0.0 70-71 0.0 0.0 0.0 2.6190680448075705 0.0 72-73 0.0 0.0 0.0 2.871006943162198 0.0 74-75 0.0 0.0 0.0 3.1393364993531794 0.0 76-77 0.0 0.0 0.0 3.4451060677249483 0.0 78-79 0.0 0.0 0.0 3.793144784901733 0.0 80-81 0.0 0.0 0.0 4.176862299725762 0.0 82-83 0.0 0.0 0.0 4.546376471537353 0.0 84-85 0.0 0.0 0.0 4.912226180851734 0.0 86-87 0.0 0.0 0.0 5.324861702049088 0.0 88-89 0.0 0.0 0.0 5.78019247234183 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCACCG 20 0.002085695 71.15131 9 CGGAGAT 25 0.0049860533 57.10273 1 ATGTATT 135 0.0 49.348038 1 GACATAT 570 0.0 49.25528 1 CATATAG 665 0.0 42.79778 3 GTATGGT 215 0.0 42.0524 1 GGTATGC 250 0.0 39.844734 8 CATGGGT 1125 0.0 38.369 4 ACGCACC 50 0.0016270907 37.947365 7 CGCACCT 50 0.0016270907 37.947365 8 CATGGAT 115 2.5393092E-9 37.12242 4 ACATATA 790 0.0 36.031097 2 ATGGATG 80 1.08822005E-5 35.575657 5 AGACGCA 55 0.0025975916 34.49761 5 GACGCAC 55 0.0025975916 34.49761 6 GGGTATG 510 0.0 34.413055 7 GTGGTAC 285 0.0 33.39341 1 GTACAGG 250 0.0 32.358215 1 TACAGGG 235 0.0 32.30022 2 GGATGAC 900 0.0 32.149853 8 >>END_MODULE