FastQCFastQC Report
Thu 26 May 2016
SRR936359_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936359_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2197486
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53650.2441426247994299No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38550.17542773878877954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25580.11640574729486332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31100.049.165191
GTATCAA60050.044.0670931
ATCAACG70600.037.27493
TCAACGC71650.036.8590974
CAACGCA73950.035.77675
AACGCAG76150.034.742316
TATCAAC78350.033.5259172
GTACATG98250.032.4263531
TACATGG102500.030.5213852
ACGCAGA90100.029.2588377
ACATGGG103850.028.9410083
CGCAGAG91750.028.681078
GAGTACA75050.025.7350541
GCAGAGT102000.025.6133029
AGTACAT71150.025.4771292
CATGGGG44050.024.712044
CATGGGA56350.021.2497224
GTATAGG7400.021.1104661
GTACGGG1605.1245086E-520.7106481
CATGGGC23800.020.681554
Produced by FastQC (version 0.11.5)