##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936359_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2197486 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7545895628004 33.0 31.0 34.0 30.0 34.0 2 31.843996275744193 34.0 31.0 34.0 30.0 34.0 3 31.91474939999618 34.0 31.0 34.0 30.0 34.0 4 35.406029435454876 37.0 35.0 37.0 33.0 37.0 5 35.397012768226965 37.0 35.0 37.0 33.0 37.0 6 35.488393555180785 37.0 35.0 37.0 33.0 37.0 7 35.43324553603527 37.0 35.0 37.0 33.0 37.0 8 35.42972696981915 37.0 35.0 37.0 33.0 37.0 9 37.10355242308711 39.0 37.0 39.0 34.0 39.0 10-11 37.02324064863212 39.0 37.0 39.0 33.0 39.0 12-13 36.930794780945135 39.0 37.0 39.0 33.0 39.0 14-15 38.179772476366175 40.0 38.0 41.0 33.0 41.0 16-17 38.13070367683798 40.0 38.0 41.0 33.0 41.0 18-19 38.075921757863306 40.0 38.0 41.0 33.0 41.0 20-21 38.005246449806734 40.0 38.0 41.0 33.0 41.0 22-23 37.84365770703431 40.0 38.0 41.0 33.0 41.0 24-25 38.004094679101485 40.0 38.0 41.0 33.0 41.0 26-27 37.96358566106906 40.0 38.0 41.0 33.0 41.0 28-29 37.831211893955185 40.0 38.0 41.0 33.0 41.0 30-31 37.67940910658817 40.0 38.0 41.0 32.5 41.0 32-33 37.50364370922045 40.0 37.0 41.0 32.0 41.0 34-35 37.242535333558436 40.0 37.0 41.0 31.5 41.0 36-37 37.0433142691239 40.0 37.0 41.0 31.0 41.0 38-39 36.885979023302085 40.0 37.0 41.0 30.5 41.0 40-41 36.70664272718916 40.0 36.0 41.0 30.0 41.0 42-43 36.51730272684331 40.0 36.0 41.0 30.0 41.0 44-45 36.33645447570542 40.0 36.0 41.0 30.0 41.0 46-47 36.05569432524257 39.5 35.5 41.0 28.5 41.0 48-49 35.882269329588446 39.0 35.0 41.0 28.0 41.0 50-51 35.56858724014624 39.5 35.0 40.5 28.0 41.0 52-53 35.59187862857829 39.0 35.0 40.5 28.0 41.0 54-55 35.66968367489031 39.0 35.0 41.0 28.0 41.0 56-57 35.52921110760205 39.0 35.0 41.0 28.0 41.0 58-59 35.30994577439856 39.0 35.0 41.0 27.0 41.0 60-61 35.02972919963995 38.5 35.0 41.0 26.5 41.0 62-63 34.73722585718407 38.0 34.5 40.0 26.0 41.0 64-65 34.41753258041234 37.0 34.0 40.0 26.0 41.0 66-67 34.071534471664435 37.0 34.0 40.0 26.0 41.0 68-69 33.70222677186567 36.0 34.0 39.0 25.0 41.0 70-71 33.2991971279908 36.0 33.5 39.0 24.0 41.0 72-73 32.89875703417451 35.0 33.0 38.5 23.0 40.0 74-75 32.481839929810704 35.0 33.0 37.5 22.0 39.5 76-77 32.08604901237141 35.0 32.5 37.0 20.5 39.0 78-79 31.697920259787775 35.0 32.0 36.5 20.0 39.0 80-81 31.30256097194703 35.0 32.0 36.0 18.5 37.5 82-83 30.95628504572953 35.0 32.0 35.5 17.5 37.0 84-85 30.641337191681767 35.0 31.0 35.0 13.5 37.0 86-87 30.323344494572435 34.5 31.0 35.0 7.5 36.0 88-89 30.005017551875188 34.0 31.0 35.0 4.5 36.0 90-91 29.663930054616955 34.0 31.0 35.0 2.0 36.0 92-93 29.36264281092121 34.0 30.0 35.0 2.0 35.0 94-95 29.007109942907487 34.0 30.0 35.0 2.0 35.0 96-97 28.603426597484578 34.0 29.5 35.0 2.0 35.0 98-99 28.126063374237653 34.0 29.0 35.0 2.0 35.0 100-101 24.865622352087794 30.5 23.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.343125726507445 1101 2 0.2714713974506999 1101 3 0.30398221201913245 1101 4 0.15571461083023763 1101 5 0.31786415460823747 1101 6 0.3104324671885479 1101 7 0.28587156579187933 1101 8 0.2871880221532592 1101 9 0.36210881069317225 1101 10-11 0.45263485932819947 1101 12-13 0.3618143221980219 1101 14-15 0.4385282112059343 1101 16-17 0.5474422644066479 1101 18-19 0.5358443186214217 1101 20-21 0.5702761155964282 1101 22-23 0.555562384104725 1101 24-25 0.5018423163766599 1101 26-27 0.5100631460515856 1101 28-29 0.30451980590290617 1101 30-31 0.4813173175467256 1101 32-33 0.5712595070410558 1101 34-35 0.28032225211889994 1101 36-37 0.1378880129296789 1101 38-39 0.09001172104716204 1101 40-41 -0.0391243038594169 1101 42-43 0.017335605814267296 1101 44-45 0.09266339914691457 1101 46-47 -0.096405615116403 1101 48-49 0.08532154565902772 1101 50-51 0.1702648345669573 1101 52-53 0.09905992268659958 1101 54-55 0.10952545100408173 1101 56-57 0.21256179446333334 1101 58-59 0.20048742864693736 1101 60-61 0.03731918453238592 1101 62-63 0.029322139389876156 1101 64-65 0.08913698860792607 1101 66-67 0.18191446034823855 1101 68-69 0.25212287106784714 1101 70-71 0.33828436581751475 1101 72-73 0.3372075492375828 1101 74-75 0.21139110049148968 1101 76-77 0.22187780231293175 1101 78-79 0.28538961427266685 1101 80-81 0.2478244688659501 1101 82-83 0.41305326849148116 1101 84-85 0.3266071751789532 1101 86-87 0.34454741635850894 1101 88-89 0.3207879460020706 1101 90-91 0.2698748091176064 1101 92-93 0.34475299433404416 1101 94-95 0.657749317592252 1101 96-97 0.7699437222222763 1101 98-99 0.7574936327511779 1101 100-101 1.0035896065389487 1102 1 0.3229812030691832 1102 2 0.3023604742802597 1102 3 0.2827651053070106 1102 4 0.22367608592956856 1102 5 0.296079922243095 1102 6 0.32090612172422794 1102 7 0.21626047083191935 1102 8 0.17299534479159462 1102 9 0.3216644925213643 1102 10-11 0.36792665924186707 1102 12-13 0.4222137548521303 1102 14-15 0.4537761827681521 1102 16-17 0.46222475244745453 1102 18-19 0.44475237599031203 1102 20-21 0.3872685216187506 1102 22-23 0.3969136628354235 1102 24-25 0.4443126496483245 1102 26-27 0.41151818124641437 1102 28-29 0.30628009785699106 1102 30-31 0.37818964710069025 1102 32-33 0.26411471490636984 1102 34-35 0.28375253640853515 1102 36-37 0.18008931941098894 1102 38-39 0.13498368411681838 1102 40-41 0.23729494185245414 1102 42-43 0.24481411010020082 1102 44-45 0.21741837884050597 1102 46-47 0.37987972286845917 1102 48-49 0.2728617400014244 1102 50-51 0.20946367873183647 1102 52-53 0.35363822346801754 1102 54-55 0.27827891983089614 1102 56-57 0.3821753120959883 1102 58-59 0.3304263772950904 1102 60-61 0.2693049612412537 1102 62-63 0.4089351284393956 1102 64-65 0.23690460584325734 1102 66-67 0.3461232154468661 1102 68-69 0.40807967261131495 1102 70-71 0.5111398599986998 1102 72-73 0.44745622557732645 1102 74-75 0.5034327308180622 1102 76-77 0.5952623201129796 1102 78-79 0.5089329272555503 1102 80-81 0.4630079092373691 1102 82-83 0.4956983425371675 1102 84-85 0.6116626984322053 1102 86-87 0.5233703938672924 1102 88-89 0.46670221862763484 1102 90-91 0.5959076385350208 1102 92-93 0.6453213313234123 1102 94-95 0.7343696451376012 1102 96-97 0.8212421646639783 1102 98-99 0.7735080498561295 1102 100-101 0.866661247172896 1103 1 0.18748186007160683 1103 2 0.1892275143415354 1103 3 0.2299962262737445 1103 4 0.16772481836982678 1103 5 0.15786925404729857 1103 6 0.2000745428659556 1103 7 0.22206848858896677 1103 8 0.0979804273453766 1103 9 0.14973801542969767 1103 10-11 0.26475598778750253 1103 12-13 0.234857507192892 1103 14-15 0.24051528721355453 1103 16-17 0.34345252174967555 1103 18-19 0.18559229698074375 1103 20-21 0.37103204518302135 1103 22-23 0.40728049447564274 1103 24-25 0.455320085095984 1103 26-27 0.45671500160862166 1103 28-29 0.3450293546296166 1103 30-31 0.32765706253146476 1103 32-33 0.38541986978331266 1103 34-35 0.17760617827556047 1103 36-37 0.3876392664064454 1103 38-39 0.3381557309385599 1103 40-41 0.30547719284219 1103 42-43 0.17491463358618375 1103 44-45 0.28225967731427204 1103 46-47 0.3641307083720875 1103 48-49 0.31728769339551377 1103 50-51 0.18390935277823672 1103 52-53 0.37102432188930123 1103 54-55 0.46030270945470164 1103 56-57 0.45671070241467504 1103 58-59 0.6078250180089171 1103 60-61 0.6093972113634436 1103 62-63 0.4550305662399907 1103 64-65 0.41215735604902903 1103 66-67 0.40452554637718663 1103 68-69 0.3431214679100947 1103 70-71 0.38811324769783795 1103 72-73 0.48737533810565026 1103 74-75 0.4622699687841276 1103 76-77 0.31382036379041267 1103 78-79 0.35575429453999874 1103 80-81 0.46482035250346243 1103 82-83 0.5277113408169001 1103 84-85 0.5438572187569619 1103 86-87 0.4973259610370633 1103 88-89 0.32815141149857396 1103 90-91 0.3488063575390008 1103 92-93 0.5056365073386964 1103 94-95 0.529507086658036 1103 96-97 0.581178268269344 1103 98-99 0.47117677569462657 1103 100-101 0.5150014961355502 1104 1 0.35335914256341283 1104 2 0.30249238480903884 1104 3 0.3134807357549114 1104 4 0.31980456467403684 1104 5 0.35321512103753605 1104 6 0.356416963714409 1104 7 0.43699480091638065 1104 8 0.357480661566818 1104 9 0.47555923002996536 1104 10-11 0.4154917491731851 1104 12-13 0.4525489553350255 1104 14-15 0.6230820273543429 1104 16-17 0.6101613551920408 1104 18-19 0.5202234021064882 1104 20-21 0.5375288602383534 1104 22-23 0.5931822833256675 1104 24-25 0.67452554268894 1104 26-27 0.6421661152109479 1104 28-29 0.6913261399079573 1104 30-31 0.6344770305445451 1104 32-33 0.6721870588614891 1104 34-35 0.6471528521495742 1104 36-37 0.6679543422262313 1104 38-39 0.5645896304324012 1104 40-41 0.7666624466962091 1104 42-43 0.7284870163107584 1104 44-45 0.7726812395894243 1104 46-47 0.8398031936560884 1104 48-49 0.8352100428933227 1104 50-51 0.7391076680439213 1104 52-53 0.7639171667486053 1104 54-55 0.7881674607104969 1104 56-57 0.8347898843048682 1104 58-59 0.8530564199707769 1104 60-61 0.7381078573632891 1104 62-63 0.8432647907906556 1104 64-65 0.8275128927792608 1104 66-67 0.9189526013136557 1104 68-69 0.7675920574270378 1104 70-71 0.7295467607133332 1104 72-73 0.823449511859053 1104 74-75 0.8663621259713565 1104 76-77 0.8467344416141742 1104 78-79 0.6924378849808619 1104 80-81 0.8388450134530174 1104 82-83 0.9897185744464281 1104 84-85 0.8957154014372328 1104 86-87 0.9266615087961867 1104 88-89 0.8716464363032195 1104 90-91 1.0062625622801846 1104 92-93 0.8745741795355464 1104 94-95 0.7024335784774074 1104 96-97 0.8661020334710408 1104 98-99 0.9027795765991407 1104 100-101 1.0391622285872266 1105 1 0.3334530512642928 1105 2 0.3201326849308721 1105 3 0.2660855914238134 1105 4 0.19690399008386805 1105 5 0.1301171190351127 1105 6 0.17895394617112004 1105 7 0.2924581320918165 1105 8 0.2716148157049503 1105 9 0.22775817333090487 1105 10-11 0.33820756226451465 1105 12-13 0.3068266162825921 1105 14-15 0.2919432813199734 1105 16-17 0.2837215625109266 1105 18-19 0.2755863106827121 1105 20-21 0.31727340082758104 1105 22-23 0.41092908769559955 1105 24-25 0.44048308574299 1105 26-27 0.3698675736004091 1105 28-29 0.2877790810134684 1105 30-31 0.44935741808129137 1105 32-33 0.4007766390367209 1105 34-35 0.25918838506284914 1105 36-37 0.3161239166917511 1105 38-39 0.3014610593484761 1105 40-41 0.2743984548828067 1105 42-43 0.28344903867514404 1105 44-45 0.29882428801342087 1105 46-47 0.32740567697929635 1105 48-49 0.32448397443305765 1105 50-51 0.16901339745979982 1105 52-53 0.1788723434883579 1105 54-55 0.408886934853939 1105 56-57 0.49328494258817557 1105 58-59 0.5147292654586977 1105 60-61 0.39248448332480024 1105 62-63 0.25492331173364136 1105 64-65 0.22560657228742542 1105 66-67 0.10550441696229029 1105 68-69 0.15528335292882645 1105 70-71 0.134582818357309 1105 72-73 0.3395467820531408 1105 74-75 0.28678048310185744 1105 76-77 0.36255276014381366 1105 78-79 0.4185958108269645 1105 80-81 0.2807735596072014 1105 82-83 0.3693903865566526 1105 84-85 0.23891164468710357 1105 86-87 0.30450405138783765 1105 88-89 0.3142884453181374 1105 90-91 0.2988765756055294 1105 92-93 0.08773949182068108 1105 94-95 0.2176819309034279 1105 96-97 0.30900314083375946 1105 98-99 0.37621232059891696 1105 100-101 0.5288354882304098 1106 1 0.2168238267494189 1106 2 0.08611575284779249 1106 3 0.214491975027169 1106 4 0.235918841867381 1106 5 0.17583267403765035 1106 6 0.16255190203376912 1106 7 0.0854476683794374 1106 8 0.08635004411989655 1106 9 0.21482847967931207 1106 10-11 0.23484437890068222 1106 12-13 0.18214327870440883 1106 14-15 0.23476882389904574 1106 16-17 0.19851093704067324 1106 18-19 0.2235856263224818 1106 20-21 0.260255573438009 1106 22-23 0.08222097802688211 1106 24-25 0.23588781093931033 1106 26-27 0.21219714943315893 1106 28-29 0.2587039783108267 1106 30-31 0.3125542698298034 1106 32-33 0.23544142889730324 1106 34-35 0.22893630913775098 1106 36-37 0.323168987256679 1106 38-39 0.2879020507764736 1106 40-41 0.4592101051436188 1106 42-43 0.46195623903909677 1106 44-45 0.49132539134987496 1106 46-47 0.5131608743258482 1106 48-49 0.4846703216408912 1106 50-51 0.46806399345590677 1106 52-53 0.5138937734901603 1106 54-55 0.4584740099721927 1106 56-57 0.44007168174777433 1106 58-59 0.4065456683291231 1106 60-61 0.5374591260284873 1106 62-63 0.5896917520339002 1106 64-65 0.582340035364048 1106 66-67 0.5331707485775894 1106 68-69 0.6974904681365999 1106 70-71 0.8276702210741433 1106 72-73 0.7191482297083311 1106 74-75 0.5767365732274428 1106 76-77 0.6879033489463495 1106 78-79 0.6912914902197542 1106 80-81 0.8337272690535116 1106 82-83 0.7307165483650735 1106 84-85 0.7580952389003706 1106 86-87 0.6897809985579499 1106 88-89 0.5659091833773111 1106 90-91 0.779523592514284 1106 92-93 0.657271074462038 1106 94-95 0.9079715008257985 1106 96-97 0.8638667888626266 1106 98-99 0.842326768182172 1106 100-101 1.0041800499968367 1107 1 0.017235618571781686 1107 2 0.16214735703318794 1107 3 0.2602938359450597 1107 4 0.18904127460676534 1107 5 0.08147672005326712 1107 6 -0.07058819732162647 1107 7 0.04104213970724402 1107 8 0.027189800701741262 1107 9 0.14588566955423232 1107 10-11 0.1408636473585645 1107 12-13 0.1358469585626665 1107 14-15 0.2830349974799873 1107 16-17 -0.026162964525887844 1107 18-19 0.11358995284069096 1107 20-21 0.23705616472882696 1107 22-23 0.1567434514561299 1107 24-25 0.15685365462324086 1107 26-27 0.06352742501692177 1107 28-29 0.21861118994710438 1107 30-31 0.22083916590323582 1107 32-33 0.1723833273631925 1107 34-35 7.405444900427938E-4 1107 36-37 0.08108728817116884 1107 38-39 0.13636291767681286 1107 40-41 0.27336194017966875 1107 42-43 -0.15780667806429705 1107 44-45 0.04376665500222998 1107 46-47 0.18493935659355998 1107 48-49 0.057549233212526474 1107 50-51 0.24286073723589396 1107 52-53 0.27062704621790346 1107 54-55 0.3440003901443802 1107 56-57 0.33472250855055563 1107 58-59 0.4404038924479181 1107 60-61 0.39250698813616935 1107 62-63 0.48150252866967236 1107 64-65 0.22943083816495147 1107 66-67 0.2958299348316302 1107 68-69 0.31275831309272917 1107 70-71 0.2910794659164466 1107 72-73 0.1772901961623674 1107 74-75 0.07774596537738176 1107 76-77 0.1613949264000425 1107 78-79 0.2619933647867754 1107 80-81 0.32896101692566404 1107 82-83 0.4549241903353902 1107 84-85 0.29999203083862724 1107 86-87 0.054209936784026524 1107 88-89 0.33824856782708324 1107 90-91 0.5451410355935273 1107 92-93 0.018264293674867815 1107 94-95 0.2111146647982629 1107 96-97 0.3283859916501406 1107 98-99 0.2674504473708481 1107 100-101 0.3820172029418316 1108 1 0.016941841748277398 1108 2 0.06928793752037521 1108 3 0.04572309848863654 1108 4 0.035514099632891316 1108 5 0.08320157792112326 1108 6 0.028893184047134923 1108 7 0.032746218238798974 1108 8 0.10449659117141863 1108 9 0.11842062504296536 1108 10-11 0.08861139955818942 1108 12-13 0.06888857406340776 1108 14-15 -0.005832862248375648 1108 16-17 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-2.33930421821146 2316 98-99 -2.2889319922155558 2316 100-101 -2.230994325591471 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17319.0 3 3903.0 4 3239.0 5 2819.0 6 2660.0 7 2831.0 8 3283.0 9 4072.0 10 4723.0 11 5827.0 12 7658.0 13 11043.0 14 11451.0 15 9990.0 16 12110.0 17 15110.0 18 13896.0 19 9884.0 20 9464.0 21 9819.0 22 10541.0 23 11494.0 24 13123.0 25 15221.0 26 18114.0 27 21538.0 28 25890.0 29 31922.0 30 38528.0 31 48190.0 32 61034.0 33 81112.0 34 114016.0 35 169874.0 36 282026.0 37 478335.0 38 513107.0 39 102218.0 40 102.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.55046857301034 17.996627294150215 12.362414726268135 24.090489406571315 2 15.989468606069432 19.222342575230744 39.59936101654804 25.188827802151792 3 19.97610665987034 23.471770587525857 28.324758279042356 28.22736447356145 4 12.473411299549186 14.923257979340681 36.490118605855564 36.11321211525457 5 13.715001397167981 37.88910218076532 33.90844772361742 14.48744869844928 6 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22.738287110263418 28-29 22.564414863100566 26.55766871458835 28.15973106434807 22.718185357963012 30-31 22.684628912543626 26.32067974898551 28.176117030863423 22.81857430760744 32-33 22.41347216381487 26.421210151774154 28.122411492382167 23.04290619202881 34-35 23.141680545732473 26.440236362391918 27.839291825420364 22.578791266455248 36-37 23.02853649413751 26.246711179110306 27.69523273008943 23.029519596662755 38-39 22.823327365592665 26.469334188066817 27.88604579758957 22.821292648750948 40-41 23.044649143487483 26.265400543911117 28.0268118498809 22.663138462720504 42-43 22.891034596292748 26.262726958859883 27.824830402484004 23.02140804236337 44-45 22.952255112616136 26.546362283405 27.667492661633858 22.833889942345003 46-47 22.948079831021836 26.71006687956647 27.314027511093997 23.027825778317702 48-49 23.176506132803574 26.772502479812143 27.1597351238145 22.89125626356978 50-51 23.11408592167242 26.80646202415974 26.970329741464592 23.10912231270325 52-53 23.36897106624108 26.703576032195176 27.088130544524063 22.839322357039677 54-55 22.971333884302815 26.702726658614033 27.184215136049367 23.141724321033784 56-57 23.092395237719565 27.065695734047697 26.933890373338 22.90801865489474 58-59 23.2143306303756 26.582021451401864 27.126721323395913 23.07692659482662 60-61 23.10975471725293 26.825432430572278 27.33748711803119 22.7273257341436 62-63 23.02837306251092 26.81502266863369 27.39952769487845 22.75707657397694 64-65 22.888751725751376 26.594351861867622 27.282251168777055 23.234645243603943 66-67 22.846077432821556 27.111844233576477 26.99309668414501 23.04898164945696 68-69 22.98011364026175 26.65302306605506 27.311991137834212 23.05487215584898 70-71 22.782610485063067 26.774603268965407 27.354840627361494 23.087945618610032 72-73 22.963295460158164 26.810233806336427 27.214995362655014 23.0114753708504 74-75 22.790471104687263 26.954405793356116 27.10084057636255 23.15428252559407 76-77 22.908554750347342 26.875584812571855 27.112139497063474 23.103720940017325 78-79 22.844832120106485 26.95053446160305 27.114465289150747 23.090168129139716 80-81 22.838687606747605 27.32848408513145 26.950372611877892 22.882455696243056 82-83 23.051433092617067 27.18995394529231 26.78927341495429 22.96933954713633 84-85 22.907328714293588 27.030232155568907 26.78189340688063 23.28054572325688 86-87 22.954217977246998 26.955145495237666 26.985102313969872 23.105534213545468 88-89 22.962548743810537 27.01775932850755 26.876713895273706 23.1429780324082 90-91 22.89126087749242 27.109299596893095 27.000132912497136 22.999306613117344 92-93 23.025603242944772 26.794918099495657 27.064850889639096 23.114627767920474 94-95 22.74756527380365 26.769354040940037 27.15825623090733 23.324824454348985 96-97 23.012093941507505 26.64237956866169 27.09168173751508 23.25384475231572 98-99 22.773775486225848 26.68788099616067 26.897357451317983 23.640986066295504 100-101 22.70555840676304 26.81833820451654 26.90356200271354 23.572541386006883 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 172.0 1 134.5 2 210.5 3 327.0 4 267.5 5 255.0 6 526.5 7 996.0 8 1512.5 9 1748.0 10 1574.0 11 1513.5 12 1724.0 13 1946.5 14 2176.0 15 2415.0 16 2607.0 17 2817.5 18 2873.5 19 2913.5 20 3102.0 21 3358.0 22 3746.0 23 4395.5 24 5358.5 25 6770.5 26 8800.0 27 11183.5 28 13595.5 29 16514.0 30 20310.0 31 24718.0 32 30301.5 33 34870.0 34 38769.0 35 43309.5 36 48588.5 37 54046.0 38 57529.5 39 59382.5 40 60798.0 41 64601.5 42 68747.0 43 72515.5 44 75871.5 45 78388.5 46 79624.5 47 80944.0 48 85841.5 49 87783.0 50 85623.0 51 83223.0 52 81888.5 53 81899.0 54 80064.5 55 74216.0 56 66671.0 57 60603.0 58 55291.5 59 48787.0 60 41528.0 61 34180.0 62 28680.5 63 23294.0 64 18379.5 65 14888.0 66 10922.5 67 8575.0 68 6186.0 69 3908.0 70 3100.5 71 2613.0 72 2269.5 73 2127.5 74 1795.0 75 1436.0 76 1035.0 77 604.5 78 356.0 79 204.5 80 170.0 81 152.0 82 80.0 83 41.5 84 30.5 85 27.5 86 25.5 87 22.0 88 16.5 89 13.0 90 20.0 91 20.0 92 16.5 93 18.5 94 16.5 95 15.5 96 21.5 97 19.5 98 10.0 99 7.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04687174343772839 2 0.011194610568622507 3 0.01005694689294949 4 0.005642811831338175 5 0.008373204652953421 6 0.005824838019445858 7 0.006097877301607382 8 0.010147959987003331 9 0.015199186706991535 10-11 0.0022298208043191174 12-13 0.009237829046464915 14-15 0.008509724294034182 16-17 0.018748697375091352 18-19 0.011444896577270571 20-21 0.003936316317828646 22-23 0.009965933798895647 24-25 0.02161560983778736 26-27 0.006939748421605416 28-29 0.01731524114374335 30-31 0.014653108142668484 32-33 0.012468793885376288 34-35 0.2701268631518016 36-37 0.4792749532875295 38-39 0.47552066315780855 40-41 0.49672671407235364 42-43 0.48651049426480986 44-45 0.5020282268009898 46-47 0.5082171171966511 48-49 0.5157029441825796 50-51 0.5268065416571482 52-53 0.515134112344743 54-55 0.5022557595361244 56-57 0.49731829918370357 58-59 0.4946106596356018 60-61 0.4917209938993923 62-63 0.4898324721977751 64-65 0.5060100496658454 66-67 0.477522951226993 68-69 0.5057142571101705 70-71 0.48730685883778097 72-73 0.49556629712316713 74-75 0.509150001410703 76-77 0.4954752840291132 78-79 0.4957028167642479 80-81 0.49882001523559194 82-83 0.49856972922694387 84-85 0.49859248250045735 86-87 0.5008223033047764 88-89 0.5147245534215007 90-91 0.5386837504311746 92-93 0.5265790089220136 94-95 0.49813741703018816 96-97 0.46946829240322807 98-99 0.46089030828865346 100-101 0.46887670729187814 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2197486.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.92333816490938 #Duplication Level Percentage of deduplicated Percentage of total 1 70.11126450010076 32.89854573307635 2 14.90921703237661 13.991804651684689 3 5.960928846212132 8.39120040083325 4 2.936996120413022 5.512546485886687 5 1.6700260211453921 3.9181597867201665 6 1.040612199081644 2.9297398869622793 7 0.6899866689440944 2.2663534457300187 8 0.48874321386775066 1.8346770480096875 9 0.3412266145346681 1.4410342642209675 >10 1.6438492064523091 13.535878961111639 >50 0.12397853405241852 4.042906173094641 >100 0.0771499234696136 6.5829565137593224 >500 0.00475862658271645 1.5621686895235007 >1k 0.0011653779386244258 0.8475474885927302 >5k 9.711482821870214E-5 0.2444804707941286 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5365 0.2441426247994299 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3855 0.17542773878877954 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2558 0.11640574729486332 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.2753273513460382E-5 0.0 14-15 0.0 0.0 0.0 1.1376636756730191E-4 0.0 16-17 0.0 0.0 0.0 1.8202618810768306E-4 0.0 18-19 0.0 0.0 0.0 1.8202618810768306E-4 0.0 20-21 0.0 0.0 0.0 2.2753273513460381E-4 0.0 22-23 0.0 0.0 0.0 3.868056497288265E-4 0.0 24-25 2.2753273513460382E-5 0.0 0.0 8.191178464845737E-4 0.0 26-27 4.5506547026920764E-5 0.0 0.0 0.001069403855132638 0.0 28-29 4.5506547026920764E-5 0.0 0.0 0.001615482419455687 0.0 30-31 4.5506547026920764E-5 0.0 0.0 0.0033902377535055964 0.0 32-33 4.5506547026920764E-5 0.0 0.0 0.007008008242145798 0.0 34-35 4.5506547026920764E-5 0.0 0.0 0.012355027517808988 0.0 36-37 4.5506547026920764E-5 0.0 0.0 0.021251557461571993 0.0 38-39 4.5506547026920764E-5 0.0 0.0 0.03526757394586359 0.0 40-41 4.5506547026920764E-5 0.0 0.0 0.05276484127771462 0.0 42-43 4.5506547026920764E-5 0.0 0.0 0.06932922439551378 0.0 44-45 4.5506547026920764E-5 0.0 0.0 0.0887832732495224 0.0 46-47 4.5506547026920764E-5 0.0 0.0 0.11292449644730387 0.0 48-49 4.5506547026920764E-5 0.0 0.0 0.13836265623535257 0.0 50-51 4.5506547026920764E-5 0.0 0.0 0.16958014749582023 0.0 52-53 4.5506547026920764E-5 0.0 0.0 0.1995234554395341 0.0 54-55 4.5506547026920764E-5 0.0 0.0 0.23026312795621906 0.0 56-57 4.5506547026920764E-5 0.0 0.0 0.2699220836901805 0.0 58-59 4.5506547026920764E-5 0.0 0.0 0.3162022420165589 0.0 60-61 4.5506547026920764E-5 0.0 0.0 0.36789767943914087 0.0 62-63 4.5506547026920764E-5 0.0 0.0 0.42289234152117466 0.0 64-65 4.5506547026920764E-5 0.0 0.0 0.47638528755132004 0.0 66-67 4.5506547026920764E-5 0.0 0.0 0.5391843224484707 0.0 68-69 4.5506547026920764E-5 0.0 0.0 0.6149072167012668 0.0 70-71 4.5506547026920764E-5 0.0 0.0 0.7041910619680853 0.0 72-73 4.5506547026920764E-5 0.0 0.0 0.7934749072349039 0.0 74-75 4.5506547026920764E-5 0.0 0.0 0.8799600998595668 0.0 76-77 4.5506547026920764E-5 0.0 0.0 0.9778674357879868 0.0 78-79 4.5506547026920764E-5 0.0 0.0 1.0964574973401424 0.0 80-81 4.5506547026920764E-5 0.0 0.0 1.2324310598565815 0.0 82-83 4.5506547026920764E-5 0.0 0.0 1.3705661833567995 0.0 84-85 4.5506547026920764E-5 0.0 0.0 1.5059709140354023 0.0 86-87 4.5506547026920764E-5 4.5506547026920764E-5 0.0 1.6519104103507372 0.0 88-89 4.5506547026920764E-5 4.5506547026920764E-5 0.0 1.8221731560519612 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3110 0.0 49.16519 1 GTATCAA 6005 0.0 44.067093 1 ATCAACG 7060 0.0 37.2749 3 TCAACGC 7165 0.0 36.859097 4 CAACGCA 7395 0.0 35.7767 5 AACGCAG 7615 0.0 34.74231 6 TATCAAC 7835 0.0 33.525917 2 GTACATG 9825 0.0 32.426353 1 TACATGG 10250 0.0 30.521385 2 ACGCAGA 9010 0.0 29.258837 7 ACATGGG 10385 0.0 28.941008 3 CGCAGAG 9175 0.0 28.68107 8 GAGTACA 7505 0.0 25.735054 1 GCAGAGT 10200 0.0 25.613302 9 AGTACAT 7115 0.0 25.477129 2 CATGGGG 4405 0.0 24.71204 4 CATGGGA 5635 0.0 21.249722 4 GTATAGG 740 0.0 21.110466 1 GTACGGG 160 5.1245086E-5 20.710648 1 CATGGGC 2380 0.0 20.68155 4 >>END_MODULE