FastQCFastQC Report
Thu 26 May 2016
SRR936359_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936359_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2197486
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55410.2521517770761679No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43600.19840854503737454No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28640.13033075068510105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32950.047.4979021
GTATCAA59000.040.958651
TCAACGC73900.032.351394
ATCAACG74150.032.1783453
CAACGCA76950.031.131465
AACGCAG79000.030.323626
TATCAAC80850.029.6344782
GTACATG97550.029.2101271
TACATGG101200.027.7540992
ACATGGG100950.026.8309863
GCACCGT7200.025.6950136
ACGCAGA96100.024.879057
CGCAGAG97600.024.3508728
GTATAGG6150.023.9784181
TAGACCG2002.0854714E-823.7184755
TGCACCG7150.023.2208845
GAGTACA76700.023.0097791
AGTACAT72650.022.2692032
CATGGGG42200.022.0318264
TAGCGCG2205.7265424E-821.5617584
Produced by FastQC (version 0.11.5)