##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936359_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2197486 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49900158635823 34.0 31.0 34.0 31.0 34.0 2 32.62944428314902 34.0 31.0 34.0 31.0 34.0 3 32.71479681781818 34.0 31.0 34.0 31.0 34.0 4 36.194492706665706 37.0 35.0 37.0 35.0 37.0 5 36.161021276130995 37.0 35.0 37.0 35.0 37.0 6 36.19824017081338 37.0 37.0 37.0 35.0 37.0 7 36.14662755530638 37.0 36.0 37.0 35.0 37.0 8 36.12172364238043 37.0 36.0 37.0 35.0 37.0 9 37.89290079663761 39.0 38.0 39.0 35.0 39.0 10-11 37.8295217807986 39.0 38.0 39.0 35.0 39.0 12-13 37.77497740599941 39.0 38.0 39.0 35.0 39.0 14-15 39.13168457045916 41.0 38.5 41.0 36.0 41.0 16-17 39.06251734937105 40.0 38.0 41.0 36.0 41.0 18-19 39.085352307136425 40.0 38.5 41.0 36.0 41.0 20-21 39.04768403530216 40.0 39.0 41.0 35.5 41.0 22-23 38.931352691211686 40.0 38.0 41.0 35.0 41.0 24-25 39.08082076518349 40.0 39.0 41.0 35.0 41.0 26-27 39.008611658959374 40.0 39.0 41.0 35.0 41.0 28-29 38.920380835190755 40.0 38.0 41.0 35.0 41.0 30-31 38.79913091596488 40.0 38.0 41.0 35.0 41.0 32-33 38.650044641922634 40.0 38.0 41.0 35.0 41.0 34-35 38.512334777104385 40.0 38.0 41.0 34.0 41.0 36-37 38.369182101728974 40.0 38.0 41.0 34.0 41.0 38-39 38.24277833851956 40.0 38.0 41.0 34.0 41.0 40-41 38.10176606358357 40.0 38.0 41.0 33.0 41.0 42-43 38.1168230423311 40.0 38.0 41.0 33.5 41.0 44-45 37.98523835874268 40.0 38.0 41.0 33.0 41.0 46-47 37.778125776455454 40.0 37.5 41.0 33.0 41.0 48-49 37.549620793943625 40.0 37.0 41.0 33.0 41.0 50-51 37.350996092807875 40.0 37.0 41.0 32.5 41.0 52-53 37.14252582269011 40.0 36.5 41.0 32.0 41.0 54-55 36.95430118781189 40.0 36.0 41.0 31.0 41.0 56-57 36.72056522771931 39.0 35.5 41.0 31.0 41.0 58-59 36.476850591994676 39.0 35.0 41.0 31.0 41.0 60-61 36.21096334629664 39.0 35.0 41.0 30.5 41.0 62-63 35.92674469825974 38.0 35.0 40.0 30.0 41.0 64-65 35.57127303655177 37.5 35.0 40.0 29.5 41.0 66-67 35.23336576433252 37.0 35.0 40.0 29.0 41.0 68-69 34.885258199597175 36.5 34.0 39.0 29.0 41.0 70-71 34.5125511607355 36.0 34.0 39.0 29.0 41.0 72-73 34.10723731573262 35.5 34.0 38.5 28.0 40.0 74-75 33.67973425086667 35.0 34.0 37.5 27.5 39.5 76-77 32.95088137080282 35.0 33.0 37.0 26.0 39.0 78-79 32.9927280537851 35.0 33.0 36.5 27.0 39.0 80-81 32.819584971189805 35.0 33.0 36.0 27.0 38.5 82-83 32.53653561387877 35.0 33.0 36.0 26.0 37.0 84-85 32.250814112126314 35.0 33.0 35.0 26.0 37.0 86-87 31.85290008673548 35.0 33.0 35.0 25.5 36.5 88-89 31.365134066838195 35.0 32.0 35.0 24.5 36.0 90-91 31.1567102133984 35.0 32.0 35.0 23.5 36.0 92-93 30.882248396576816 34.0 32.0 35.0 21.5 35.5 94-95 30.739659319786337 34.0 32.0 35.0 20.0 35.0 96-97 30.510535675767674 34.0 32.0 35.0 19.0 35.0 98-99 30.085842412647906 34.0 31.0 35.0 8.5 35.0 100-101 26.83123350956502 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06671034431405332 1101 2 0.042225538228805704 1101 3 0.16296024255581187 1101 4 0.12616320784247392 1101 5 0.08462134174490643 1101 6 0.1464255416899718 1101 7 0.11571729980001066 1101 8 0.15171461919176465 1101 9 0.24789706773029963 1101 10-11 0.16348376248758 1101 12-13 0.24938048484768416 1101 14-15 0.38039555772977707 1101 16-17 0.3202683600763834 1101 18-19 0.32151821853220497 1101 20-21 0.21937561831237673 1101 22-23 0.29634718385297987 1101 24-25 0.2439230750163901 1101 26-27 0.24497334507861268 1101 28-29 0.270488060595234 1101 30-31 0.3123441614201141 1101 32-33 0.3507028249519877 1101 34-35 0.3169780117384349 1101 36-37 0.2677018956731345 1101 38-39 0.324283345063769 1101 40-41 0.3462098004633489 1101 42-43 0.4439365233856236 1101 44-45 0.4738711690988566 1101 46-47 0.39857164794246813 1101 48-49 0.38829973932440254 1101 50-51 0.33155883220481996 1101 52-53 0.366302171908103 1101 54-55 0.3465140282435968 1101 56-57 0.2677996007257164 1101 58-59 0.14356408551916644 1101 60-61 0.31535840062582565 1101 62-63 0.15653586753586524 1101 64-65 0.25463571036231514 1101 66-67 0.2016560263452405 1101 68-69 0.2913321275251377 1101 70-71 0.2706107325394811 1101 72-73 0.3072716528301882 1101 74-75 0.18153474292037686 1101 76-77 0.07191038907785696 1101 78-79 0.02450510063653155 1101 80-81 0.2471759260745401 1101 82-83 0.2503300462856899 1101 84-85 0.34699722369805386 1101 86-87 0.3482627520165664 1101 88-89 0.3244752021515076 1101 90-91 0.5839288975533208 1101 92-93 0.6977565579238956 1101 94-95 0.484355723181924 1101 96-97 0.43374829342366183 1101 98-99 0.4917200045503094 1101 100-101 0.7377178813161649 1102 1 0.10352726073625007 1102 2 0.10320531111425169 1102 3 0.17725180901192772 1102 4 0.11953279316900023 1102 5 0.13347123294487773 1102 6 0.12361717950480511 1102 7 0.14215526616450092 1102 8 0.15754885552128428 1102 9 0.20578440904523632 1102 10-11 0.19138799643847193 1102 12-13 0.24695726154774178 1102 14-15 0.32477319513937175 1102 16-17 0.35932790056144626 1102 18-19 0.46408589919751364 1102 20-21 0.33473338099616257 1102 22-23 0.389339823332854 1102 24-25 0.292143621923735 1102 26-27 0.18898372733232094 1102 28-29 0.31341506766943183 1102 30-31 0.3066073826057334 1102 32-33 0.2601317569497681 1102 34-35 0.20211854877889124 1102 36-37 0.11590823690878693 1102 38-39 0.22777122289058838 1102 40-41 0.20431991754451673 1102 42-43 0.2558798592737688 1102 44-45 0.14468390971430267 1102 46-47 0.3295089579829025 1102 48-49 0.3009855142713249 1102 50-51 0.10138069968903096 1102 52-53 0.13229291289356127 1102 54-55 0.20819538511650393 1102 56-57 0.20547304206620254 1102 58-59 0.1161752201228623 1102 60-61 0.12303452893056743 1102 62-63 0.2900890033553054 1102 64-65 0.2943212914954145 1102 66-67 0.28529769983944675 1102 68-69 0.16376629690079625 1102 70-71 0.15510981192386453 1102 72-73 0.05537701266316475 1102 74-75 0.17239025472100167 1102 76-77 0.06476460584980259 1102 78-79 0.19708033563578198 1102 80-81 0.13685236712430537 1102 82-83 0.26275945841315007 1102 84-85 0.3007676732580151 1102 86-87 0.3749936415338766 1102 88-89 0.3636663378123721 1102 90-91 0.43887192069644954 1102 92-93 0.32574745748429024 1102 94-95 0.3618147359648276 1102 96-97 0.4349128281383585 1102 98-99 0.4330687504868287 1102 100-101 0.9307487329516846 1103 1 -0.0314690151920729 1103 2 -0.010938279356359715 1103 3 0.06197592089488779 1103 4 -0.043072395986328615 1103 5 -0.04423152265657393 1103 6 -0.01061444505798903 1103 7 0.0907054173491133 1103 8 0.0709827035241446 1103 9 0.03189089210388829 1103 10-11 0.056025264451029955 1103 12-13 0.06360743262228397 1103 14-15 0.09327426686795803 1103 16-17 0.12529737057949575 1103 18-19 0.007723396790623838 1103 20-21 0.03915740887592278 1103 22-23 0.1104125411222796 1103 24-25 0.01333721334652438 1103 26-27 0.004899928313697899 1103 28-29 0.05597287541846896 1103 30-31 0.028761350752219528 1103 32-33 0.08631639790482382 1103 34-35 0.15785724273002444 1103 36-37 0.17972290298016702 1103 38-39 0.2451747778978799 1103 40-41 0.17640538181564125 1103 42-43 0.19673959084438053 1103 44-45 0.24213847498183583 1103 46-47 0.18075292488098427 1103 48-49 0.18809668204287533 1103 50-51 0.19400349354684465 1103 52-53 0.2225376436495239 1103 54-55 0.17048796913558562 1103 56-57 0.250122492659834 1103 58-59 0.31029787855990065 1103 60-61 0.18301482943775937 1103 62-63 0.15588991152095844 1103 64-65 0.11721814317368029 1103 66-67 0.23006347477432598 1103 68-69 0.15669056070895238 1103 70-71 0.03121751165365083 1103 72-73 0.026092399427049884 1103 74-75 0.199393741806297 1103 76-77 0.11551830717501588 1103 78-79 0.06397161919210248 1103 80-81 0.17840657987460418 1103 82-83 0.27554005980839946 1103 84-85 0.28849212263497037 1103 86-87 0.12729896886073533 1103 88-89 0.1563114549407736 1103 90-91 0.2556921612376257 1103 92-93 0.2183920985239851 1103 94-95 0.11843104655240566 1103 96-97 0.13947452500315904 1103 98-99 0.1970288616588327 1103 100-101 0.3826240992754997 1104 1 0.08070515633064446 1104 2 0.0728906886738443 1104 3 0.04825386470560744 1104 4 0.06382803214867039 1104 5 0.019502646645840116 1104 6 0.08194400525205481 1104 7 0.12669640747549948 1104 8 0.1553950204073331 1104 9 0.10730422939365525 1104 10-11 0.16434554657502787 1104 12-13 0.159579991103719 1104 14-15 0.19512466810510176 1104 16-17 0.1794211982402416 1104 18-19 0.27544381783932437 1104 20-21 0.27461700425125457 1104 22-23 0.22519168042203574 1104 24-25 0.20987234422779721 1104 26-27 0.2916678955361789 1104 28-29 0.31870072255771476 1104 30-31 0.20741303935132294 1104 32-33 0.13117628783372481 1104 34-35 0.26596509145669955 1104 36-37 0.29448712784726894 1104 38-39 0.44859920945048515 1104 40-41 0.4709182193811259 1104 42-43 0.40265512172296525 1104 44-45 0.3355748017671729 1104 46-47 0.25939270787210233 1104 48-49 0.3148461449936022 1104 50-51 0.4344067694306517 1104 52-53 0.3774354395816957 1104 54-55 0.3524371739499088 1104 56-57 0.2908207371143803 1104 58-59 0.4435378432217689 1104 60-61 0.3261482505803528 1104 62-63 0.31964065529723484 1104 64-65 0.24990912702303802 1104 66-67 0.15681895537440482 1104 68-69 0.33197016092952936 1104 70-71 0.3176578801205494 1104 72-73 0.2860988165238112 1104 74-75 0.37384137606601797 1104 76-77 0.3306877896001126 1104 78-79 0.29444006725589844 1104 80-81 0.36831814636728666 1104 82-83 0.30899831988809723 1104 84-85 0.44876469102477046 1104 86-87 0.5397688870985178 1104 88-89 0.5968783486353679 1104 90-91 0.610830222195375 1104 92-93 0.5768586717787478 1104 94-95 0.39029949554997856 1104 96-97 0.42177322036640064 1104 98-99 0.4151856400998746 1104 100-101 0.5676032683522507 1105 1 -0.013221136095140196 1105 2 0.04428783944646142 1105 3 0.053518763237569544 1105 4 0.13306132219004496 1105 5 0.026518155353159045 1105 6 0.09759746586106388 1105 7 0.13979356508369278 1105 8 0.16378317235004403 1105 9 0.13382222906393793 1105 10-11 0.1408902351342931 1105 12-13 0.15209252633707848 1105 14-15 0.09727495821483245 1105 16-17 0.1844543909752332 1105 18-19 0.06388109543191689 1105 20-21 0.1602596385199888 1105 22-23 0.03564126003434609 1105 24-25 0.13131628851386523 1105 26-27 0.2256515967171282 1105 28-29 0.06846750891391906 1105 30-31 0.18498080198808253 1105 32-33 0.15995831952665185 1105 34-35 0.13532976521895534 1105 36-37 0.22038173337443112 1105 38-39 0.24920079007606688 1105 40-41 0.33685706513443137 1105 42-43 0.29849708329096103 1105 44-45 0.2945432736950835 1105 46-47 0.38447488552460385 1105 48-49 0.27628195695907465 1105 50-51 0.21716248014922712 1105 52-53 0.21446760497038042 1105 54-55 0.1665714286111708 1105 56-57 0.18152596713139246 1105 58-59 0.2304891377585392 1105 60-61 0.21663333495946802 1105 62-63 0.19683256197961185 1105 64-65 0.0674493563765921 1105 66-67 0.1436089605614015 1105 68-69 0.28216296427815024 1105 70-71 0.14929371299482597 1105 72-73 0.08368872440441777 1105 74-75 0.2872628212668431 1105 76-77 0.22248093110852096 1105 78-79 0.05629978522822654 1105 80-81 0.13582424812081229 1105 82-83 0.2403694251197166 1105 84-85 0.23286364196547282 1105 86-87 0.1509846539492088 1105 88-89 0.2154255181052882 1105 90-91 0.3616891922756764 1105 92-93 0.4444249868825487 1105 94-95 0.21339965879952771 1105 96-97 0.25330375527003923 1105 98-99 0.3619460814365887 1105 100-101 0.5171250798593334 1106 1 0.04845998897752679 1106 2 0.04220073516512457 1106 3 0.058396531056693846 1106 4 0.01545877994733047 1106 5 0.06198580475074067 1106 6 0.026358871054810606 1106 7 0.06469426480314411 1106 8 0.012422180105474467 1106 9 -0.07938222938742712 1106 10-11 0.07303978242610754 1106 12-13 0.09573803745890075 1106 14-15 0.11335572244301062 1106 16-17 0.05927042055826348 1106 18-19 0.05911159553981804 1106 20-21 0.1301831486269336 1106 22-23 -0.026309866535363824 1106 24-25 0.17353798526063002 1106 26-27 0.021090710042905414 1106 28-29 0.1463961138105887 1106 30-31 0.13427082685446834 1106 32-33 0.11469880215614836 1106 34-35 0.1987703959968954 1106 36-37 0.12124828993427883 1106 38-39 0.21690474692248785 1106 40-41 0.03527219766117895 1106 42-43 0.09981437952367145 1106 44-45 0.06787373608541003 1106 46-47 0.14338141105493918 1106 48-49 0.06691025603226564 1106 50-51 0.02662174321768873 1106 52-53 0.09495208632448282 1106 54-55 0.1199000537275765 1106 56-57 0.2890716219299847 1106 58-59 0.1281418016112994 1106 60-61 0.0987867949642478 1106 62-63 0.171310008227195 1106 64-65 0.33720576204667196 1106 66-67 0.20474048312681958 1106 68-69 0.24928062174419807 1106 70-71 0.11943266534041186 1106 72-73 0.0020322275511190924 1106 74-75 0.25850948452467293 1106 76-77 0.2806113299233317 1106 78-79 0.08721507815190677 1106 80-81 0.05866846479081289 1106 82-83 0.010767175479884372 1106 84-85 0.06600344587292994 1106 86-87 0.19359920165903333 1106 88-89 0.27019487218199245 1106 90-91 0.4204243604202631 1106 92-93 0.4398048262523133 1106 94-95 0.32728718944505886 1106 96-97 0.34094475875211216 1106 98-99 0.21173897497774874 1106 100-101 0.5795420865998935 1107 1 0.013111562619762651 1107 2 0.029669541741434102 1107 3 0.015154526745625674 1107 4 0.0174010775860296 1107 5 -0.0525425403817934 1107 6 0.024729473393705348 1107 7 0.057545981117471 1107 8 0.055099419271797956 1107 9 0.042530297685502205 1107 10-11 0.047821975713311815 1107 12-13 0.09425826521918168 1107 14-15 0.11990962493668178 1107 16-17 0.16333090940331374 1107 18-19 0.12382227251114841 1107 20-21 0.06506402773423048 1107 22-23 -0.006335645373020782 1107 24-25 0.17498208947821325 1107 26-27 0.21389710092837078 1107 28-29 0.07469849622844293 1107 30-31 0.011254522576621184 1107 32-33 0.03726700276237693 1107 34-35 0.13366440917732092 1107 36-37 0.15056585894610208 1107 38-39 0.09789163087651076 1107 40-41 0.05267510209405657 1107 42-43 -0.06685481952963812 1107 44-45 -0.06058264977821182 1107 46-47 -0.06300586898152716 1107 48-49 -0.07767940719464406 1107 50-51 -0.04147031375843824 1107 52-53 -0.019292974877728852 1107 54-55 0.08883153249544051 1107 56-57 -0.02586181922293207 1107 58-59 -0.031161628957100618 1107 60-61 0.04527497558608218 1107 62-63 0.004986029272615156 1107 64-65 0.060946500593637154 1107 66-67 -0.15606898782756673 1107 68-69 0.125320466916655 1107 70-71 0.21637667555923912 1107 72-73 0.0828669107753015 1107 74-75 0.17623494634185732 1107 76-77 -0.013087276577259388 1107 78-79 0.04568372248377983 1107 80-81 0.22676541485301271 1107 82-83 0.26666823727956057 1107 84-85 0.18750894544414365 1107 86-87 0.15078495608277365 1107 88-89 0.3573711237175239 1107 90-91 0.02294108061042266 1107 92-93 0.20933351905149777 1107 94-95 0.27705283637542166 1107 96-97 -0.16067845816869664 1107 98-99 0.013185139442096983 1107 100-101 0.26748050067343954 1108 1 -0.07578817028793594 1108 2 -0.03494727593162139 1108 3 -0.03839735611920503 1108 4 0.033215116115513865 1108 5 0.015194389761582272 1108 6 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-0.9071783598828347 2316 64-65 -0.8078513526636684 2316 66-67 -0.8522024446374985 2316 68-69 -0.8075976520583978 2316 70-71 -0.820722086271914 2316 72-73 -0.9374345374009891 2316 74-75 -0.912009559192704 2316 76-77 -0.7641378606604476 2316 78-79 -0.8823039614550297 2316 80-81 -0.9676203498511562 2316 82-83 -1.1358726339596288 2316 84-85 -0.9892351006991547 2316 86-87 -1.1480598207114099 2316 88-89 -1.080787337102965 2316 90-91 -1.115756253461825 2316 92-93 -1.0735680107943537 2316 94-95 -0.9897193201384553 2316 96-97 -0.9189653662466881 2316 98-99 -1.1996311478014121 2316 100-101 -1.091435700525352 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 15.0 4 21.0 5 50.0 6 69.0 7 176.0 8 361.0 9 662.0 10 1046.0 11 1724.0 12 2800.0 13 4146.0 14 5445.0 15 7127.0 16 9262.0 17 12848.0 18 12054.0 19 7725.0 20 6627.0 21 6780.0 22 7298.0 23 8177.0 24 9654.0 25 11863.0 26 14719.0 27 18693.0 28 23518.0 29 29634.0 30 37232.0 31 46026.0 32 57965.0 33 75850.0 34 105642.0 35 159660.0 36 269533.0 37 505233.0 38 613913.0 39 123803.0 40 133.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.72831029496383 18.46201372674013 12.768715699690942 24.040960278605095 2 16.15794959559041 19.473789615981573 39.19040704951684 25.177853738911164 3 19.892959500083276 23.536486694340713 28.73547317252533 27.83508063305068 4 12.621422844104583 15.281508050563234 36.38535126048585 35.71171784484633 5 13.656605775872974 37.875099090506154 34.04608721056698 14.42220792305389 6 34.15830635553537 35.1629543942487 16.561334179148353 14.117405071067573 7 30.274004020958493 31.84680129930293 19.700284779971295 18.17890989976728 8 27.710847759667185 34.92845915741898 18.911246761071514 18.449446321842323 9 27.09773805157348 15.551634913715038 19.605813188343408 37.744813846368075 10-11 26.028457629259005 26.1592894580263 26.654379851443306 21.15787306127139 12-13 26.25417352489189 23.04213502448028 27.577346445911072 23.12634500471676 14-15 22.80733529132836 24.656539336314314 25.46077654192109 27.075348830436237 16-17 23.07382161237021 28.28157615588451 25.937542804849024 22.707059426896258 18-19 22.804309105951074 26.559054301142304 28.2626146423686 22.374021950538022 20-21 23.320607412979438 25.849181575197267 28.356617922273365 22.473593089549933 22-23 22.912943660593815 25.86261112135009 28.25978334323101 22.964661874825083 24-25 22.755071717837787 26.122549467992396 28.379139094697397 22.74323971947242 26-27 22.611423735917377 26.62089660506222 28.071862015392046 22.695817643628352 28-29 22.42351444248105 26.619599798128824 28.28501425066702 22.6718715087231 30-31 22.56524981017844 26.311236366921147 28.34394824748681 22.779565575413596 32-33 22.287239561527734 26.40178516169666 28.335551057408416 22.975424219367195 34-35 22.947867660911804 26.412139365426956 28.081713953380422 22.558279020280818 36-37 22.9502292053615 26.232733943167265 27.885920417604954 22.931116433866283 38-39 22.634762012027384 26.507439186160543 28.082262222495263 22.77553657931681 40-41 22.901692079948194 26.232040166136283 28.22558001850307 22.64068773541245 42-43 22.751369343097583 26.24094158379615 27.982018782162953 23.02567029094331 44-45 22.76076789860014 26.56917870419102 27.848622883549396 22.82143051365944 46-47 22.778568816458723 26.741600545972194 27.428116430071892 23.051714207497195 48-49 23.065747847114036 26.807384236155013 27.32288897475704 22.803978941973917 50-51 23.036046870146848 26.796603632333984 27.128913383067538 23.03843611445163 52-53 23.306851145437683 26.66500100576792 27.21928835320749 22.80885949558691 54-55 22.851052104539 26.706929516652394 27.334035199011232 23.107983179797372 56-57 22.972151368767392 27.022660717251863 27.14098156242676 22.864206351553985 58-59 23.08408518554587 26.597456520294777 27.247547397861716 23.07091089629764 60-61 23.03544863822384 26.820089184745488 27.461260973459822 22.68320120357085 62-63 22.932796841481583 26.80230954827471 27.44716007291969 22.817733537324013 64-65 22.792549167281955 26.589934572560065 27.39390367797549 23.223612582182483 66-67 22.756914037222536 27.154279936254426 27.090024691852417 22.998781334670618 68-69 22.829701759192094 26.75759936582076 27.405567088937083 23.00713178605006 70-71 22.7045897510925 26.76960413345632 27.43459221300005 23.09121390245112 72-73 22.902570795630574 26.833282365212074 27.26469110588081 22.99945573327654 74-75 22.770702520971692 26.942469713117628 27.136850928743115 23.149976837167564 76-77 22.806661570487883 26.92262008032751 27.134689816662956 23.136028532521653 78-79 22.79950536376013 26.958631728104045 27.12180975455647 23.12005315357936 80-81 22.725286967158507 27.346682357481168 26.948518838078805 22.97951183728152 82-83 22.92469667611079 27.220673988366705 26.836211925809767 23.018417409712736 84-85 22.820304363862327 27.06450627164849 26.75555986354298 23.359629500946202 86-87 22.829253601318054 26.94293022628525 27.027244991689265 23.20057118070743 88-89 22.865420296343704 27.07719612913799 26.864645721089737 23.19273785342857 90-91 22.775581717559014 27.158977767572274 26.990936933786614 23.074503581082098 92-93 22.96054188526541 26.85055547986518 27.043622987082962 23.145279647786445 94-95 22.666270750068545 26.805738060213454 27.10549698341132 23.42249420630668 96-97 22.94010515978298 26.696354539737094 27.032749344912983 23.330790955566943 98-99 22.629779106509847 26.759583356182137 26.88766558365244 23.722971953655577 100-101 22.479252831155485 26.89547085657883 27.038089510725637 23.587186801540046 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 177.0 1 123.5 2 190.5 3 321.5 4 251.5 5 241.0 6 502.5 7 918.5 8 1360.0 9 1527.0 10 1299.5 11 1139.5 12 1220.5 13 1383.5 14 1567.5 15 1718.5 16 1840.5 17 1982.0 18 2127.0 19 2349.5 20 2698.5 21 3108.0 22 3757.5 23 4624.0 24 5684.5 25 7187.5 26 9254.0 27 11652.0 28 14034.0 29 17014.0 30 20799.5 31 25154.0 32 30735.5 33 35371.0 34 39460.0 35 43855.5 36 48943.5 37 54443.5 38 57719.5 39 59564.0 40 61693.0 41 65772.5 42 69345.0 43 72765.0 44 76088.0 45 78675.5 46 79472.0 47 80547.5 48 85886.0 49 88235.5 50 85894.5 51 83558.5 52 81994.5 53 81735.5 54 80437.0 55 74412.5 56 66742.0 57 60686.0 58 55192.0 59 48475.5 60 40914.0 61 33643.5 62 28553.0 63 23005.0 64 17895.5 65 14531.5 66 10674.5 67 8578.0 68 6152.5 69 3690.0 70 2888.5 71 2454.0 72 2124.0 73 2011.5 74 1759.5 75 1460.5 76 1035.0 77 536.0 78 288.0 79 169.5 80 117.5 81 101.0 82 49.5 83 13.0 84 8.0 85 4.0 86 3.5 87 2.5 88 0.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2729027625204438 2 0.02075098544427587 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.868056497288265E-4 12-13 1.3651964108076227E-4 14-15 0.0 16-17 5.915851113499699E-4 18-19 0.0 20-21 1.1376636756730191E-4 22-23 4.5506547026920764E-5 24-25 0.002502860086480642 26-27 0.00238909371891334 28-29 0.0020477946162114342 30-31 0.001251430043240321 32-33 0.001729248787022989 34-35 0.0018202618810768305 36-37 1.1376636756730191E-4 38-39 4.5506547026920764E-5 40-41 5.915851113499699E-4 42-43 0.003731536856207503 44-45 0.003936316317828646 46-47 0.013856743569697372 48-49 0.014516588501587723 50-51 0.006302656763228526 52-53 0.007281047524307322 54-55 4.3231219675574724E-4 56-57 0.003230964838911374 58-59 0.0010921571286460982 60-61 0.0011376636756730192 62-63 0.0 64-65 6.143383848634304E-4 66-67 0.0 68-69 0.0 70-71 1.3651964108076227E-4 72-73 0.001547222598915306 74-75 0.0 76-77 0.19747566082332266 78-79 0.10637155367542728 80-81 0.0010011440345922568 82-83 0.0 84-85 7.963645729711133E-4 86-87 0.12441489957160136 88-89 0.5944520238126659 90-91 0.3161339821960185 92-93 0.2977265839236291 94-95 0.002434600265940261 96-97 0.003094445197830612 98-99 0.003162705018370993 100-101 7.963645729711133E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2197486.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.11950323165151 #Duplication Level Percentage of deduplicated Percentage of total 1 68.23721370939016 30.78829184479724 2 15.803026200445458 14.260493834417446 3 6.264263269791254 8.47921340535787 4 3.1563657158276874 5.696546124622454 5 1.7790584585568228 4.013511693507575 6 1.1592886379701994 3.138391646838797 7 0.7381442498782795 2.3313291307465605 8 0.5118394775221629 1.8475154368118432 9 0.380535936974468 1.5452633194271137 >10 1.745477464658581 13.93551452204084 >50 0.13451596189782655 4.250425761698462 >100 0.08391947161588759 6.94710831073991 >500 0.004637563359991007 1.4208615043984971 >1k 0.0016130655165185965 1.0934847517042912 >5k 1.0081659478241228E-4 0.25204871289127984 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5541 0.2521517770761679 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4360 0.19840854503737454 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2864 0.13033075068510105 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.2753273513460382E-5 0.0 14-15 0.0 0.0 0.0 9.101309405384153E-5 0.0 16-17 0.0 0.0 0.0 1.365196410807623E-4 0.0 18-19 0.0 0.0 0.0 1.365196410807623E-4 0.0 20-21 0.0 0.0 0.0 1.8202618810768306E-4 0.0 22-23 0.0 0.0 0.0 3.412991027019057E-4 0.0 24-25 0.0 0.0 0.0 7.73611299457653E-4 0.0 26-27 0.0 0.0 0.0 0.0010466505816191776 0.0 28-29 0.0 0.0 0.0 0.0015927291459422268 0.0 30-31 0.0 0.0 0.0 0.0033902377535055964 0.0 32-33 0.0 0.0 0.0 0.007099021336199639 0.0 34-35 0.0 0.0 0.0 0.01251430043240321 0.0 36-37 0.0 0.0 0.0 0.021683869658327744 0.0 38-39 0.0 0.0 0.0 0.03599567869829433 0.0 40-41 0.0 0.0 0.0 0.05378873858582034 0.0 42-43 0.0 0.0 0.0 0.07062616098578103 0.0 44-45 0.0 0.0 0.0 0.09039875566897809 0.0 46-47 0.0 0.0 0.0 0.11492678451648838 0.0 48-49 0.0 0.0 0.0 0.14072899668075245 0.0 50-51 0.0 0.0 0.0 0.17212851412932778 0.0 52-53 0.0 0.0 0.0 0.20229935480817626 0.0 54-55 0.0 0.0 0.0 0.23342583297459008 0.0 56-57 0.0 0.0 0.0 0.27315304852909184 0.0 58-59 0.0 0.0 0.0 0.3196607395906049 0.0 60-61 0.0 0.0 0.0 0.3714244368337273 0.0 62-63 0.0 0.0 0.0 0.4268741643860302 0.0 64-65 0.0 0.0 0.0 0.48061739642482365 0.0 66-67 0.0 0.0 0.0 0.5436894706041357 0.0 68-69 0.0 0.0 0.0 0.6198219237801743 0.0 70-71 0.0 0.0 0.0 0.7095608345172619 0.0 72-73 0.0 0.0 0.0 0.7994590181689439 0.0 74-75 0.0 0.0 0.0 0.8864220295373896 0.0 76-77 0.0 0.0 0.0 0.9852850029533748 0.0 78-79 0.0 0.0 0.0 1.1041708570612054 0.0 80-81 0.0 0.0 0.0 1.240462965406833 0.0 82-83 0.0 0.0 0.0 1.37898489455678 0.0 84-85 0.0 0.0 0.0 1.5146399112440307 0.0 86-87 0.0 0.0 0.0 1.661148239397202 0.0 88-89 0.0 0.0 0.0 1.8328444413297742 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3295 0.0 47.497902 1 GTATCAA 5900 0.0 40.95865 1 TCAACGC 7390 0.0 32.35139 4 ATCAACG 7415 0.0 32.178345 3 CAACGCA 7695 0.0 31.13146 5 AACGCAG 7900 0.0 30.32362 6 TATCAAC 8085 0.0 29.634478 2 GTACATG 9755 0.0 29.210127 1 TACATGG 10120 0.0 27.754099 2 ACATGGG 10095 0.0 26.830986 3 GCACCGT 720 0.0 25.695013 6 ACGCAGA 9610 0.0 24.87905 7 CGCAGAG 9760 0.0 24.350872 8 GTATAGG 615 0.0 23.978418 1 TAGACCG 200 2.0854714E-8 23.718475 5 TGCACCG 715 0.0 23.220884 5 GAGTACA 7670 0.0 23.009779 1 AGTACAT 7265 0.0 22.269203 2 CATGGGG 4220 0.0 22.031826 4 TAGCGCG 220 5.7265424E-8 21.561758 4 >>END_MODULE