FastQCFastQC Report
Thu 26 May 2016
SRR936358_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936358_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386188
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73560.5306639503443977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51990.37505735152807557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38190.2755037556233354No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18390.13266598758609943No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16110.11621800217575105No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14930.10770544832302689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43400.035.22711
GTATCAA67600.033.049081
TATAACG600.0040256231.546232
GTATAGG7000.029.7521761
TATCACG804.5955184E-429.5745942
GTACATG76000.029.147171
TACATGG73650.028.655032
ATCAACG79050.027.6563283
TCAACGC79650.027.5668164
CAACGCA81150.027.0572625
ACATGGG75400.026.924053
AACGCAG82750.026.7037336
CATGGGG28750.026.4997924
TATCAAC83150.026.4624122
GTGTCCG909.1497705E-426.2885259
GTGTAGC8150.026.1347891
AGTACAT53300.023.881622
CGTATAG1601.9178697E-623.6665041
ACGCAGA95700.023.0407757
CGCAGAG96350.022.837058
Produced by FastQC (version 0.11.5)