##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936358_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1386188 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.94734408319795 33.0 31.0 34.0 30.0 34.0 2 32.033743619191625 34.0 31.0 34.0 30.0 34.0 3 32.104239107538085 34.0 31.0 34.0 30.0 34.0 4 35.5936373709771 37.0 35.0 37.0 33.0 37.0 5 35.61315853260885 37.0 35.0 37.0 35.0 37.0 6 35.711425145795516 37.0 35.0 37.0 35.0 37.0 7 35.67203005652913 37.0 35.0 37.0 35.0 37.0 8 35.67631230395877 37.0 35.0 37.0 35.0 37.0 9 37.37605505169573 39.0 37.0 39.0 35.0 39.0 10-11 37.30157886231882 39.0 37.0 39.0 34.5 39.0 12-13 37.235257050270235 39.0 37.0 39.0 34.0 39.0 14-15 38.497944723226574 40.0 38.0 41.0 34.0 41.0 16-17 38.46757474455124 40.0 38.0 41.0 34.0 41.0 18-19 38.47969900186699 40.0 38.0 41.0 34.0 41.0 20-21 38.425967473387445 40.0 38.0 41.0 34.0 41.0 22-23 38.275840290061666 40.0 38.0 41.0 34.0 41.0 24-25 38.43018623736462 40.0 38.0 41.0 34.0 41.0 26-27 38.37428581115981 40.0 38.0 41.0 34.0 41.0 28-29 38.2443377088822 40.0 38.0 41.0 34.0 41.0 30-31 38.08770707869351 40.0 38.0 41.0 34.0 41.0 32-33 37.88823629983812 40.0 38.0 41.0 33.0 41.0 34-35 37.63817606269856 40.0 37.5 41.0 33.0 41.0 36-37 37.446879499750395 40.0 37.0 41.0 33.0 41.0 38-39 37.307683373395236 40.0 37.0 41.0 32.5 41.0 40-41 37.14558450946048 40.0 37.0 41.0 32.0 41.0 42-43 36.983776010180435 40.0 37.0 41.0 31.0 41.0 44-45 36.796473133514354 40.0 37.0 41.0 31.0 41.0 46-47 36.507357227158224 40.0 36.5 41.0 30.0 41.0 48-49 36.33096376537671 40.0 36.5 41.0 30.0 41.0 50-51 36.03033210502471 39.5 36.5 40.5 29.5 41.0 52-53 36.08634975919573 39.5 36.0 40.5 29.5 41.0 54-55 36.22943280420837 40.0 36.0 41.0 29.5 41.0 56-57 36.12989507916675 40.0 36.0 41.0 29.0 41.0 58-59 35.96889743671133 40.0 36.0 41.0 28.5 41.0 60-61 35.751769961938784 39.0 35.0 41.0 28.0 41.0 62-63 35.50770927175823 39.0 35.0 41.0 28.0 41.0 64-65 35.236413819770476 39.0 35.0 41.0 28.0 41.0 66-67 34.926760655841775 38.5 35.0 40.0 26.5 41.0 68-69 34.57176551809711 37.5 35.0 40.0 26.0 41.0 70-71 34.18448507706025 37.0 34.0 39.5 26.0 41.0 72-73 33.777379403082406 36.5 34.0 39.0 26.0 41.0 74-75 33.34745792057066 36.0 34.0 39.0 25.0 40.5 76-77 32.91753607735747 35.5 33.5 38.5 23.5 40.0 78-79 32.49307886087601 35.0 33.0 37.0 22.5 39.0 80-81 32.06093185051378 35.0 33.0 37.0 21.5 39.0 82-83 31.66897599748375 35.0 32.5 36.0 20.0 38.5 84-85 31.290122263358214 35.0 32.0 36.0 18.5 37.0 86-87 30.939312705058764 35.0 32.0 35.5 16.5 37.0 88-89 30.60025263528468 35.0 32.0 35.0 9.0 36.5 90-91 30.241281485628214 34.5 31.0 35.0 4.5 36.0 92-93 29.940893659445905 34.0 31.0 35.0 2.0 36.0 94-95 29.5943905155722 34.0 31.0 35.0 2.0 36.0 96-97 29.217031167489548 34.0 31.0 35.0 2.0 35.0 98-99 28.803439721019082 34.0 30.5 35.0 2.0 35.0 100-101 25.775090752480903 31.5 24.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2836776767039062 1101 2 0.20962489198305434 1101 3 0.2696602912297479 1101 4 0.018797007474333327 1101 5 0.24485494930225116 1101 6 0.27469919544855514 1101 7 0.2472138699789781 1101 8 0.16980054022666025 1101 9 0.08837261428947585 1101 10-11 0.2826271490091301 1101 12-13 0.26022766520276264 1101 14-15 0.44035464807689806 1101 16-17 0.4829724935076527 1101 18-19 0.4954489888773921 1101 20-21 0.620358866029072 1101 22-23 0.4916083757838621 1101 24-25 0.4031988388815506 1101 26-27 0.3611978774934457 1101 28-29 0.3881896159969429 1101 30-31 0.5299520709886352 1101 32-33 0.5697979178571373 1101 34-35 0.2578591022186387 1101 36-37 0.352632545975041 1101 38-39 0.24536847392291605 1101 40-41 0.1656522300836727 1101 42-43 0.1325319784953649 1101 44-45 0.2554441968416654 1101 46-47 0.2483491941368996 1101 48-49 0.24141351559909197 1101 50-51 0.503193726686149 1101 52-53 0.44511716559113523 1101 54-55 0.3244774886228825 1101 56-57 0.43234110431233574 1101 58-59 0.46340737237927243 1101 60-61 0.383550014779388 1101 62-63 0.2515350202323532 1101 64-65 0.15561875915253864 1101 66-67 0.30571133070285583 1101 68-69 0.3574219047363627 1101 70-71 0.4210550999760656 1101 72-73 0.48638830100416186 1101 74-75 0.3869720214233112 1101 76-77 0.327590813057121 1101 78-79 0.46929272651896525 1101 80-81 0.4806747297888734 1101 82-83 0.6304824069833188 1101 84-85 0.6250006396451511 1101 86-87 0.6023065586656813 1101 88-89 0.6065511761318554 1101 90-91 0.6975821878817037 1101 92-93 0.7155384474950246 1101 94-95 0.7342165912640866 1101 96-97 0.7474063328936822 1101 98-99 0.8321731342903469 1101 100-101 1.1357724113202572 1102 1 0.3714627089982052 1102 2 0.33065199111251786 1102 3 0.3900604597217452 1102 4 0.35170560056615585 1102 5 0.35268982714555364 1102 6 0.3660464293716075 1102 7 0.2743186158368829 1102 8 0.30096875140329615 1102 9 0.3035930579850685 1102 10-11 0.3394591484474887 1102 12-13 0.38749633973239384 1102 14-15 0.42567548492048246 1102 16-17 0.4994155572537977 1102 18-19 0.48132479623486546 1102 20-21 0.5275057348861338 1102 22-23 0.38635949906384326 1102 24-25 0.5546605069523167 1102 26-27 0.4612410535675835 1102 28-29 0.5034170801923992 1102 30-31 0.5599545281635798 1102 32-33 0.3312044750040073 1102 34-35 0.2057469848358764 1102 36-37 0.2154705830432846 1102 38-39 0.05593994261148083 1102 40-41 0.08305078386069908 1102 42-43 0.19109593524909485 1102 44-45 0.18958313253388326 1102 46-47 0.24488563053116508 1102 48-49 0.07257189807592113 1102 50-51 0.07974911843004406 1102 52-53 0.0941406140606631 1102 54-55 0.1331906309986195 1102 56-57 0.19758857693238951 1102 58-59 0.19059566218550827 1102 60-61 0.22227509200488527 1102 62-63 0.27888653666031615 1102 64-65 0.1677417583100791 1102 66-67 0.32833208330043817 1102 68-69 0.34872210131035786 1102 70-71 0.4123988236491911 1102 72-73 0.22829577081601116 1102 74-75 0.23286678413041528 1102 76-77 0.26329391611805164 1102 78-79 0.36482488040832095 1102 80-81 0.4812005652283631 1102 82-83 0.39648493436326504 1102 84-85 0.4414651159158609 1102 86-87 0.05737009418940886 1102 88-89 0.16257827526131408 1102 90-91 0.3481417217205163 1102 92-93 0.4144990624908118 1102 94-95 0.29360089342640094 1102 96-97 0.20991370441853263 1102 98-99 0.25911430250152634 1102 100-101 0.3668869156729677 1103 1 0.31454259545400376 1103 2 0.322089541801887 1103 3 0.3592534848636717 1103 4 0.2782364197064666 1103 5 0.31538244677248173 1103 6 0.33037124688986097 1103 7 0.4044402703380996 1103 8 0.24195009771310794 1103 9 0.3610139825592782 1103 10-11 0.4822419951628163 1103 12-13 0.44331649382809246 1103 14-15 0.4578672935173955 1103 16-17 0.4809859141070021 1103 18-19 0.518896775148086 1103 20-21 0.5278706983897834 1103 22-23 0.5234732460224834 1103 24-25 0.6192874331485854 1103 26-27 0.6175072741677425 1103 28-29 0.6200229196084521 1103 30-31 0.7109449985609828 1103 32-33 0.6954228448336934 1103 34-35 0.6857966604238754 1103 36-37 0.6395287744463616 1103 38-39 0.6098045006001271 1103 40-41 0.6852354959450437 1103 42-43 0.6865734291663657 1103 44-45 0.8990569768161265 1103 46-47 0.8528043247728547 1103 48-49 0.7708565696087675 1103 50-51 0.7939391022094426 1103 52-53 0.8351037121952984 1103 54-55 0.7653013365947317 1103 56-57 0.8747367926663685 1103 58-59 0.8534687359892317 1103 60-61 0.8985930157680642 1103 62-63 0.6746620841055773 1103 64-65 0.689225943224919 1103 66-67 0.726115943803272 1103 68-69 0.7809524338326668 1103 70-71 0.7146899834221045 1103 72-73 0.7995265088533259 1103 74-75 0.7268803688830587 1103 76-77 0.7037409964100263 1103 78-79 0.6349222040092997 1103 80-81 0.6872082700134428 1103 82-83 0.7813551695619694 1103 84-85 0.7571261053724712 1103 86-87 0.8480179044083904 1103 88-89 0.7522680562832136 1103 90-91 0.8758769204228649 1103 92-93 0.8136809359701296 1103 94-95 0.8519727506851211 1103 96-97 1.0332886030397823 1103 98-99 1.1462696147480784 1103 100-101 1.3301401597930003 1104 1 0.24501448893348154 1104 2 0.20460830179212763 1104 3 0.25768181894504494 1104 4 0.22585608600304852 1104 5 0.2742108627442619 1104 6 0.19100597629717697 1104 7 0.17681052522199536 1104 8 0.24446389703436466 1104 9 0.2870882036161362 1104 10-11 0.27996481187791034 1104 12-13 0.37719294167413864 1104 14-15 0.485715937994911 1104 16-17 0.5351477579010435 1104 18-19 0.2367980972057424 1104 20-21 0.47200411676315923 1104 22-23 0.5027713113615775 1104 24-25 0.6384145522597606 1104 26-27 0.4810347428879709 1104 28-29 0.4526889248525876 1104 30-31 0.5211236220147129 1104 32-33 0.5186842484667906 1104 34-35 0.4935018392048178 1104 36-37 0.5178289972827628 1104 38-39 0.47558395555646626 1104 40-41 0.611509521724777 1104 42-43 0.5974349320128454 1104 44-45 0.5246357215306503 1104 46-47 0.45533465965738174 1104 48-49 0.49985345147398164 1104 50-51 0.43824830936855363 1104 52-53 0.614278154513741 1104 54-55 0.551159886662056 1104 56-57 0.588150874667015 1104 58-59 0.46338692432143347 1104 60-61 0.5926634415194556 1104 62-63 0.525605792323752 1104 64-65 0.5318841531320899 1104 66-67 0.545168652879731 1104 68-69 0.40453116279903156 1104 70-71 0.4958939692802602 1104 72-73 0.5103588776121271 1104 74-75 0.5760504410883485 1104 76-77 0.5042364727523605 1104 78-79 0.6206986668160894 1104 80-81 0.5052216008270634 1104 82-83 0.7153684295088993 1104 84-85 0.5853324298317162 1104 86-87 0.5085634598852025 1104 88-89 0.6658549742904434 1104 90-91 0.6848488414616121 1104 92-93 0.6631600657270589 1104 94-95 0.467120310902132 1104 96-97 0.6893675879136794 1104 98-99 0.8079411633429494 1104 100-101 0.8279265596859133 1105 1 0.147418960650338 1105 2 0.142657674432634 1105 3 0.13609931777091333 1105 4 0.17229441896425612 1105 5 0.08623463285530875 1105 6 0.11832967018577989 1105 7 0.25910317835340635 1105 8 0.22767506914053826 1105 9 0.15947463345427337 1105 10-11 0.28876888939382894 1105 12-13 0.23094433074481913 1105 14-15 0.20272214101352404 1105 16-17 0.23473507879754862 1105 18-19 0.23648307009159453 1105 20-21 0.2388327240481729 1105 22-23 0.2517437840228709 1105 24-25 0.1665915140895109 1105 26-27 0.2482127030521184 1105 28-29 0.30093888828121607 1105 30-31 0.38228039387862367 1105 32-33 0.27457448557763087 1105 34-35 0.06289442073331486 1105 36-37 0.3386337471987133 1105 38-39 0.41271102376401103 1105 40-41 0.5028383202075091 1105 42-43 0.496405544025194 1105 44-45 0.5193396088754128 1105 46-47 0.5765341237904877 1105 48-49 0.5957638092575195 1105 50-51 0.502834962997845 1105 52-53 0.5278526151180287 1105 54-55 0.7124313129970403 1105 56-57 0.7470166102392852 1105 58-59 0.7613589452941554 1105 60-61 0.6247070243337234 1105 62-63 0.5407630235754723 1105 64-65 0.4649899740119068 1105 66-67 0.46634226040855253 1105 68-69 0.5480189556587618 1105 70-71 0.6382711472019267 1105 72-73 0.625586941844297 1105 74-75 0.6297558272179131 1105 76-77 0.5966956475481382 1105 78-79 0.6377643464405764 1105 80-81 0.6636305150036677 1105 82-83 0.6508584474481225 1105 84-85 0.722118036878058 1105 86-87 0.7069025419821671 1105 88-89 0.6815347912539131 1105 90-91 0.7468524010755218 1105 92-93 0.8269703154646422 1105 94-95 0.7432037218694347 1105 96-97 0.7238996282884784 1105 98-99 0.6969249025543967 1105 100-101 0.72120326513107 1106 1 0.1866710423315432 1106 2 0.2362249077791816 1106 3 0.2677167097217463 1106 4 0.1884243505661587 1106 5 0.2737835771455508 1106 6 0.19833809603827746 1106 7 0.2407769491702183 1106 8 0.18310416806995988 1106 9 0.31088472465173567 1106 10-11 0.31865185678082497 1106 12-13 0.3468973813990601 1106 14-15 0.42330569325380907 1106 16-17 0.3767853489204569 1106 18-19 0.39801750456820884 1106 20-21 0.36474531821946954 1106 22-23 0.3628959573971784 1106 24-25 0.43247300695231417 1106 26-27 0.2547566785675812 1106 28-29 0.2858389551923963 1106 30-31 0.26990244483025094 1106 32-33 0.396959683337343 1106 34-35 0.16280427650254836 1106 36-37 0.23945495804328232 1106 38-39 0.31966390094481767 1106 40-41 0.2448997421940291 1106 42-43 0.4091688519157515 1106 44-45 0.4531247992005518 1106 46-47 0.4544168805311699 1106 48-49 0.4171812730759257 1106 50-51 0.4577959934300466 1106 52-53 0.2784114473940065 1106 54-55 0.4181906309986303 1106 56-57 0.4478229519323875 1106 58-59 0.54223628718551 1106 60-61 0.5530042586715567 1106 62-63 0.48208966166031786 1106 64-65 0.37977300831007454 1106 66-67 0.4865091666337733 1106 68-69 0.46908668464369185 1106 70-71 0.592867573649194 1106 72-73 0.6241811874826837 1106 74-75 0.5048719924637552 1106 76-77 0.5258459994513913 1106 78-79 0.43797592207499036 1106 80-81 0.5687266068950265 1106 82-83 0.5673443093632642 1106 84-85 0.33815782424919405 1106 86-87 0.36817738585607884 1106 88-89 0.26322931692798335 1106 90-91 0.335980263387178 1106 92-93 0.36340531249080854 1106 94-95 0.37636131009306695 1106 96-97 0.46413245441853235 1106 98-99 0.3601559691681935 1106 100-101 0.5207410823396366 1107 1 -0.17057409828053594 1107 2 -0.02228225206366119 1107 3 0.06094135302198822 1107 4 0.015156799904453067 1107 5 -0.08734325804881848 1107 6 -0.03270253671606582 1107 7 -0.044854254303729135 1107 8 0.09483971914523437 1107 9 -0.015348215794915632 1107 10-11 0.05099037259967787 1107 12-13 0.04160407339658434 1107 14-15 0.11513164703792711 1107 16-17 -0.12286215159649316 1107 18-19 0.022942869022294587 1107 20-21 0.07246024274387253 1107 22-23 0.11917277449808239 1107 24-25 0.0790649734535549 1107 26-27 -0.004543685886510218 1107 28-29 0.03987282874491882 1107 30-31 -0.044262800206972486 1107 32-33 0.020560347626009445 1107 34-35 -0.03760185718231668 1107 36-37 -0.037586695699481254 1107 38-39 0.052048296623063095 1107 40-41 0.11534503530817375 1107 42-43 -0.11665116979639834 1107 44-45 0.15114744188045393 1107 46-47 0.28182008048981544 1107 48-49 0.10599208004449423 1107 50-51 0.13188001173029562 1107 52-53 0.019986354341895662 1107 54-55 0.06631924969175884 1107 56-57 0.11440722871071785 1107 58-59 0.19476646450146973 1107 60-61 0.09009974047469882 1107 62-63 0.18447896842210554 1107 64-65 -0.24725410604062148 1107 66-67 -0.12813814002462465 1107 68-69 -0.03413976800312213 1107 70-71 0.1058851760065167 1107 72-73 -0.05010993638001793 1107 74-75 0.06344474111966747 1107 76-77 -0.004116133509732833 1107 78-79 0.08086292838185472 1107 80-81 -0.02206866554087128 1107 82-83 0.1477545786974268 1107 84-85 5.072385630811027E-5 1107 86-87 -0.10895021184863651 1107 88-89 0.17447860611325794 1107 90-91 0.25957058855260584 1107 92-93 0.04970274321868473 1107 94-95 0.2613366254363285 1107 96-97 0.4002786754689893 1107 98-99 0.3157416804998725 1107 100-101 0.3226034169136618 1108 1 -0.11055896514229957 1108 2 -0.09139210455265712 1108 3 0.005748053144763787 1108 4 -0.11038333814833834 1108 5 -0.011286256561469088 1108 6 -0.10979437780327572 1108 7 -0.22355881316161685 1108 8 -0.08933020225888555 1108 9 -0.0672589599521487 1108 10-11 -0.11455243600692455 1108 12-13 -0.20466920585803905 1108 14-15 -0.24898445528878455 1108 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-2.2706550752267773 2316 96-97 -2.3482458089767633 2316 98-99 -2.279679665058161 2316 100-101 -2.466863995571792 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7788.0 3 1621.0 4 1358.0 5 1190.0 6 1129.0 7 1259.0 8 1469.0 9 1832.0 10 2285.0 11 2936.0 12 4450.0 13 7081.0 14 7317.0 15 6438.0 16 8348.0 17 11391.0 18 10260.0 19 6257.0 20 5322.0 21 5300.0 22 5546.0 23 6101.0 24 6877.0 25 7833.0 26 9520.0 27 11456.0 28 13753.0 29 17049.0 30 20971.0 31 26189.0 32 33582.0 33 43663.0 34 61022.0 35 97120.0 36 144920.0 37 276833.0 38 402464.0 39 105994.0 40 264.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.46411572002763 19.391202489118047 13.05170289653719 23.092978894317135 2 14.663755685360053 21.038429570798606 41.788117886085004 22.50969685775633 3 19.170006529416643 26.405321654936564 29.076827064251624 25.347844751395165 4 11.05247362034361 16.899284758885685 39.99968256395975 32.04855905681095 5 11.753378819546903 39.639597391456796 35.424559236570396 13.182464552425904 6 29.940286512197005 38.10884924021675 18.734466521272704 13.216397726313541 7 27.68949409324556 33.1112273424114 22.040869329966633 17.15840923437641 8 25.25550805086641 36.249388555490306 20.876988186675632 17.618115206967655 9 26.830876724129844 15.964051662747684 21.297932378972707 35.90713923414977 10-11 23.88285763651297 27.60999004804281 28.705715323525176 19.80143699191904 12-13 24.906984747446543 25.28914630864542 28.957161398868593 20.84670754503944 14-15 21.79164832944945 27.1294739803906 27.283254092506148 23.795623597653798 16-17 20.17542150731753 30.11261841420747 28.60239306914762 21.109567009327385 18-19 19.93717988399887 29.08967468746866 30.987409281677554 19.98573614685492 20-21 21.21974208335272 28.231560106785324 30.891706534415036 19.656991275446913 22-23 20.326351745481137 28.64033453326556 30.55610167162019 20.477212049633113 24-25 19.989501255519958 28.50795162640344 31.219802580309985 20.282744537766618 26-27 20.010648682225572 28.91254300776214 30.832985231085786 20.243823078926503 28-29 19.757477103838504 28.83574290494981 31.05035532071943 20.356424670492252 30-31 20.326953359632462 28.825035038447595 31.09321760273886 19.754793999181082 32-33 19.692185369876476 29.162339154536106 31.03445787922264 20.111017596364782 34-35 20.092767008972878 29.056097130472775 31.016581451806584 19.834554408747763 36-37 20.10517227505725 28.79181645451705 30.5419692326875 20.5610420377382 38-39 19.95809345138594 29.195620435839704 30.935311250904313 19.91097486187004 40-41 20.52122837565305 28.673941155166734 31.020150398400236 19.784680070779984 42-43 19.904642385383088 28.930012600283906 30.860255746613944 20.305089267719058 44-45 20.1453449904197 29.110110884922225 30.501964096995753 20.242580027662317 46-47 20.15971783617356 28.99920017809271 30.077219433279407 20.76386255245432 48-49 20.26520913202405 29.12923932312242 30.111653835038922 20.49389770981461 50-51 20.580436565813777 29.330537196286354 29.583908407775706 20.50511783012416 52-53 20.176778279847333 29.49939048224059 29.998680163804725 20.325151074107353 54-55 20.45572702349368 29.183721414188728 30.012355552784097 20.3481960095335 56-57 20.498347229080068 29.532721528945423 29.40344359298404 20.565487648990466 58-59 20.355744551268106 29.4323240672264 29.77320514493699 20.438726236568495 60-61 20.474435593617255 29.587988234449686 29.63235917844152 20.305216993491538 62-63 20.434803255387553 29.496448776873425 29.98353891265474 20.085209055084285 64-65 20.410562819845804 29.118127450678262 29.863913145152665 20.607396584323272 66-67 20.387728760947596 29.698626899688612 29.421672606752086 20.491971732611702 68-69 20.587210527021828 29.349903323194926 29.697308965871798 20.365577183911444 70-71 20.563098765033807 28.9139917040605 29.898767498737627 20.62414203216806 72-73 20.48424740417319 29.22689639821553 29.57299455566971 20.715861641941572 74-75 20.472613952172992 29.376102448637 29.378966775619865 20.772316823570147 76-77 20.248683047115936 28.997904983630306 30.068687100115245 20.684724869138513 78-79 20.058134054897454 28.996988165826345 29.863706165632024 21.081171613644177 80-81 20.15155393172315 29.601342537843166 29.306674478077177 20.94042905235651 82-83 20.69210291526048 29.2740180985206 29.534753966777927 20.499125019440992 84-85 20.450227147404906 29.080160864809677 29.543192810461072 20.92641917732434 86-87 20.19140795933143 29.432363519954524 29.60080078396199 20.77542773675206 88-89 20.710777070673412 28.980961121265842 29.658582325646154 20.64967948241459 90-91 20.468311776015216 29.127966798478035 29.815941619520803 20.58777980598595 92-93 20.46571049462339 29.22998426526322 29.79283019647932 20.511475043634068 94-95 20.18028861404714 29.223395828310718 29.71898105936328 20.877334498278866 96-97 20.56341070571674 29.08676811915666 29.531945646516792 20.817875528609804 98-99 20.428421395194437 28.88073651167325 29.629924918079602 21.060917175052708 100-101 20.39217363381809 29.115905922978737 29.462964801937964 21.028955641265217 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1032.0 1 691.0 2 866.5 3 1268.5 4 862.5 5 1034.0 6 2540.5 7 4217.5 8 4886.0 9 4440.0 10 3292.0 11 2429.5 12 2300.5 13 2348.5 14 2302.0 15 2229.5 16 2225.5 17 2299.0 18 2397.5 19 2486.5 20 2672.5 21 3048.0 22 3606.0 23 4426.5 24 5345.5 25 6456.0 26 8288.0 27 10479.5 28 12409.5 29 15419.0 30 20089.0 31 24928.5 32 30454.5 33 36993.5 34 42961.0 35 48001.0 36 53492.0 37 57273.5 38 56787.0 39 56564.5 40 55869.0 41 53604.0 42 51597.5 43 51962.0 44 53414.0 45 53706.0 46 51690.5 47 49132.0 48 51410.0 49 51011.5 50 47560.5 51 43322.5 52 37800.5 53 33799.5 54 29807.5 55 25739.5 56 21788.0 57 17974.5 58 14645.0 59 11683.5 60 8929.5 61 6689.0 62 4862.0 63 3420.0 64 2406.0 65 1706.5 66 1271.0 67 1035.5 68 738.0 69 492.5 70 393.5 71 286.5 72 246.0 73 226.5 74 165.0 75 103.5 76 82.5 77 53.0 78 22.5 79 17.0 80 15.5 81 14.5 82 13.0 83 11.5 84 8.0 85 7.5 86 8.5 87 9.5 88 7.0 89 8.0 90 8.5 91 5.0 92 7.0 93 10.5 94 10.5 95 12.0 96 11.0 97 7.0 98 6.0 99 7.0 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04595336274733297 2 0.012408129344648777 3 0.011037463893786412 4 0.005915503524774417 5 0.009161816434711596 6 0.0049776797952370095 7 0.0045448380739120525 8 0.009089676147824105 9 0.014355917090611086 10-11 0.0020559981762935473 12-13 0.009017535860936612 14-15 0.008512553852724163 16-17 0.01940573717273559 18-19 0.012047427910211314 20-21 0.003065962192718448 22-23 0.009919289447030272 24-25 0.02221920836134781 26-27 0.007286168975636782 28-29 0.018540053730085673 30-31 0.015185530389817256 32-33 0.013814864938954887 34-35 0.274133090172473 36-37 0.48600911276103964 38-39 0.4826185192773275 40-41 0.5038638337656941 42-43 0.4944495263268763 44-45 0.5089857941347061 46-47 0.5144323857947118 48-49 0.5221874666351174 50-51 0.5324674575165851 52-53 0.5215021339096861 54-55 0.5086611628437124 56-57 0.5041884650566878 58-59 0.5026735190320505 60-61 0.4982729615319134 62-63 0.4959284022080699 64-65 0.5125928084790807 66-67 0.48467451745362095 68-69 0.5128092293397433 70-71 0.4933313518801202 72-73 0.501735695302513 74-75 0.5161998228234553 76-77 0.5022767474541693 78-79 0.5022406773107255 80-81 0.5056312707944377 82-83 0.5060280423723189 84-85 0.5062444632329813 86-87 0.5078315495445063 88-89 0.5227645889302173 90-91 0.5465708836030899 92-93 0.5332249305289037 94-95 0.5057394812247689 96-97 0.4780015409165279 98-99 0.4685511633342664 100-101 0.47576519202301565 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1386188.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.00913143350037 #Duplication Level Percentage of deduplicated Percentage of total 1 70.95847538853074 34.77613246637304 2 14.621613131759915 14.331851194884331 3 5.82980040149638 8.571403623240279 4 2.8956856215627007 5.676601488690544 5 1.6202746764630391 3.970412728857468 6 1.0113036726188118 2.9737868766334183 7 0.6939101212852135 2.3805552635972247 8 0.46084168143056936 1.8068360428212866 9 0.32279684966177113 1.423799390825424 >10 1.410759802104824 11.859220348758122 >50 0.10336837020585306 3.5253394460651086 >100 0.06660245130923928 5.8418329289032975 >500 0.003094337515847508 0.9803140613033735 >1k 0.0011787952441323839 0.9752590348362474 >5k 2.9469881103309596E-4 0.9066551042109653 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7356 0.5306639503443977 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5199 0.37505735152807557 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3819 0.2755037556233354 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1839 0.13266598758609943 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1611 0.11621800217575105 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1493 0.10770544832302689 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 7.21402868874929E-5 0.0 7 0.0 0.0 0.0 7.21402868874929E-5 0.0 8 0.0 0.0 0.0 7.21402868874929E-5 0.0 9 0.0 0.0 0.0 1.442805737749858E-4 0.0 10-11 0.0 0.0 0.0 2.524910041062251E-4 0.0 12-13 0.0 0.0 0.0 5.049820082124503E-4 0.0 14-15 0.0 0.0 0.0 7.574730123186754E-4 0.0 16-17 0.0 0.0 0.0 0.00162315645496859 0.0 18-19 0.0 3.607014344374645E-5 0.0 0.0022002787500685333 0.0 20-21 0.0 7.21402868874929E-5 0.0 0.004003785922255856 0.0 22-23 0.0 7.21402868874929E-5 0.0 0.00833220313550543 0.0 24-25 0.0 7.21402868874929E-5 0.0 0.013237742643854945 0.0 26-27 0.0 7.21402868874929E-5 0.0 0.015221600533261 0.0 28-29 0.0 7.21402868874929E-5 0.0 0.0167726167013421 0.0 30-31 0.0 7.21402868874929E-5 0.0 0.02001892961127928 0.0 32-33 0.0 7.21402868874929E-5 0.0 0.025826222705722456 0.0 34-35 0.0 7.21402868874929E-5 0.0 0.033004181251028 0.0 36-37 0.0 7.21402868874929E-5 0.0 0.045448380739120525 0.0 38-39 0.0 7.21402868874929E-5 0.0 0.06539517006351231 0.0 40-41 0.0 7.21402868874929E-5 0.0 0.09006714817903488 0.0 42-43 0.0 7.21402868874929E-5 0.0 0.1147030561511137 0.0 44-45 0.0 7.21402868874929E-5 0.0 0.1424409964593547 0.0 46-47 0.0 7.21402868874929E-5 0.0 0.1733531093906454 0.0 48-49 0.0 7.21402868874929E-5 0.0 0.21180388230167912 0.0 50-51 0.0 7.21402868874929E-5 0.0 0.2554487558686123 0.0 52-53 0.0 7.21402868874929E-5 0.0 0.29447665107474597 0.0 54-55 0.0 7.21402868874929E-5 0.0 0.3397446810966478 0.0 56-57 0.0 7.21402868874929E-5 0.0 0.39197424880319265 0.0 58-59 0.0 7.21402868874929E-5 0.0 0.45058823189928066 0.0 60-61 0.0 7.21402868874929E-5 0.0 0.5170294361226616 0.0 62-63 0.0 7.21402868874929E-5 0.0 0.5862841115346548 0.0 64-65 0.0 7.21402868874929E-5 0.0 0.6557191376638667 0.0 66-67 0.0 7.21402868874929E-5 0.0 0.7361194873999775 0.0 68-69 0.0 7.21402868874929E-5 0.0 0.832498910681668 0.0 70-71 0.0 7.21402868874929E-5 0.0 0.9416471647424447 0.0 72-73 0.0 7.21402868874929E-5 0.0 1.0518775231065338 0.0 74-75 0.0 7.21402868874929E-5 0.0 1.1636588976387041 0.0 76-77 0.0 1.442805737749858E-4 0.0 1.286441665921217 0.0 78-79 0.0 1.442805737749858E-4 0.0 1.4276202073600406 0.0 80-81 0.0 1.442805737749858E-4 0.0 1.588312696401931 0.0 82-83 0.0 1.442805737749858E-4 0.0 1.7544878472472711 0.0 84-85 0.0 1.442805737749858E-4 0.0 1.9218893829696981 0.0 86-87 0.0 1.442805737749858E-4 0.0 2.100977645167899 0.0 88-89 0.0 1.442805737749858E-4 0.0 2.305026446629173 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4340 0.0 35.2271 1 GTATCAA 6760 0.0 33.04908 1 TATAACG 60 0.00402562 31.54623 2 GTATAGG 700 0.0 29.752176 1 TATCACG 80 4.5955184E-4 29.574594 2 GTACATG 7600 0.0 29.14717 1 TACATGG 7365 0.0 28.65503 2 ATCAACG 7905 0.0 27.656328 3 TCAACGC 7965 0.0 27.566816 4 CAACGCA 8115 0.0 27.057262 5 ACATGGG 7540 0.0 26.92405 3 AACGCAG 8275 0.0 26.703733 6 CATGGGG 2875 0.0 26.499792 4 TATCAAC 8315 0.0 26.462412 2 GTGTCCG 90 9.1497705E-4 26.288525 9 GTGTAGC 815 0.0 26.134789 1 AGTACAT 5330 0.0 23.88162 2 CGTATAG 160 1.9178697E-6 23.666504 1 ACGCAGA 9570 0.0 23.040775 7 CGCAGAG 9635 0.0 22.83705 8 >>END_MODULE