FastQCFastQC Report
Thu 26 May 2016
SRR936358_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936358_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386188
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72750.5248205871065108No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53100.38306492337258724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40460.29187960074679625No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17830.1286261315203998No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17710.12776044807774992No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14940.10777758860991439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73300.031.1608541
GGTATCA48500.030.9058671
GTACATG75700.028.5385631
CGTATAC856.4545544E-427.9064943
TACATGG75700.027.202622
ACATGGG73800.027.1275353
TAACGCA909.013601E-426.3561344
TCAACGC86450.026.0665074
ATCAACG86750.025.9763643
CATGGGG27400.025.7982314
CAACGCA88000.025.6073825
GTGTAGC9150.025.4827981
AACGCAG89350.025.2204746
GTCCTAC7050.024.9738041
TATAACG950.001234556424.9725722
TATCAAC91050.024.8052652
GGCCACG1601.8802202E-623.720526
GTATAGG7100.023.4575061
CAGTCCG2252.8558134E-923.1933989
ATAGCCC8800.022.1032123
Produced by FastQC (version 0.11.5)