##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936358_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1386188 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46811182898712 34.0 31.0 34.0 31.0 34.0 2 32.596418379036606 34.0 31.0 34.0 31.0 34.0 3 32.68348016286391 34.0 31.0 34.0 31.0 34.0 4 36.16594790894165 37.0 35.0 37.0 35.0 37.0 5 36.144036739605305 37.0 35.0 37.0 35.0 37.0 6 36.188532147154646 37.0 37.0 37.0 35.0 37.0 7 36.14574141458446 37.0 36.0 37.0 35.0 37.0 8 36.123761711975575 37.0 36.0 37.0 35.0 37.0 9 37.890997469318734 39.0 38.0 39.0 35.0 39.0 10-11 37.82018203879993 39.0 38.0 39.0 35.0 39.0 12-13 37.781403027583565 39.0 38.0 39.0 35.0 39.0 14-15 39.10172321503288 40.0 38.5 41.0 36.0 41.0 16-17 39.04675664484182 40.0 38.0 41.0 36.0 41.0 18-19 39.11616750397493 40.0 38.5 41.0 36.0 41.0 20-21 39.09631233281488 40.0 39.0 41.0 36.0 41.0 22-23 38.970290465651125 40.0 38.5 41.0 35.0 41.0 24-25 39.12409932851821 40.5 39.0 41.0 36.0 41.0 26-27 39.025721258588305 40.5 39.0 41.0 35.0 41.0 28-29 38.93120954733413 40.0 38.0 41.0 35.0 41.0 30-31 38.79330689632286 40.0 38.0 41.0 35.0 41.0 32-33 38.6134784747812 40.0 38.0 41.0 35.0 41.0 34-35 38.48195374653366 40.0 38.0 41.0 34.0 41.0 36-37 38.33453579168194 40.0 38.0 41.0 34.0 41.0 38-39 38.21366257679333 40.0 38.0 41.0 34.0 41.0 40-41 38.083547830453014 40.0 38.0 41.0 33.5 41.0 42-43 38.088416217713615 40.0 38.0 41.0 34.0 41.0 44-45 37.93057795912243 40.0 38.0 41.0 33.0 41.0 46-47 37.693792616874475 40.0 38.0 41.0 33.0 41.0 48-49 37.47232229683131 40.0 38.0 41.0 33.0 41.0 50-51 37.308970356113306 40.0 37.0 41.0 32.5 41.0 52-53 37.14767657778022 40.0 37.0 41.0 32.0 41.0 54-55 37.012111993466974 40.0 37.0 41.0 31.5 41.0 56-57 36.825270453935545 40.0 36.5 41.0 31.0 41.0 58-59 36.63412177857549 40.0 36.0 41.0 31.0 41.0 60-61 36.422634952834684 39.0 36.0 41.0 31.0 41.0 62-63 36.17974762442036 39.0 35.0 41.0 30.0 41.0 64-65 35.86606398266325 39.0 35.0 40.5 29.0 41.0 66-67 35.55562809662182 38.0 35.0 40.0 29.0 41.0 68-69 35.2230491102217 37.5 35.0 40.0 29.0 41.0 70-71 34.862893417054536 37.0 34.5 39.5 28.0 41.0 72-73 34.44635323635755 36.5 34.0 39.0 28.0 41.0 74-75 33.99422408793035 36.0 34.0 39.0 27.5 40.0 76-77 33.206086764565846 35.0 33.0 37.5 26.0 39.0 78-79 33.22066090602429 35.0 34.0 37.0 26.0 39.0 80-81 33.006055455681334 35.0 34.0 37.0 26.0 39.0 82-83 32.66478536821845 35.0 33.0 36.5 26.0 38.5 84-85 32.30478116965375 35.0 33.0 36.0 26.0 37.0 86-87 31.870279139626085 35.0 33.0 36.0 25.0 37.0 88-89 31.33520741775286 35.0 32.0 35.0 23.0 36.5 90-91 31.084527495548944 35.0 32.0 35.0 21.5 36.0 92-93 30.78500715631646 34.5 32.0 35.0 20.0 36.0 94-95 30.63058185469792 34.0 32.0 35.0 19.0 36.0 96-97 30.38433314961607 34.0 32.0 35.0 14.5 35.0 98-99 29.960922688697348 34.0 31.0 35.0 2.0 35.0 100-101 26.781460379111635 31.5 25.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10049295551524295 1101 2 0.09471264982699523 1101 3 0.2594862085846259 1101 4 0.1440644524428123 1101 5 0.053396367658180566 1101 6 0.12241033063609308 1101 7 0.2155245575601299 1101 8 0.09450239349289546 1101 9 0.1621952455268385 1101 10-11 0.18595047841478163 1101 12-13 0.2688541946214258 1101 14-15 0.4379961892650357 1101 16-17 0.4034209617790552 1101 18-19 0.38109378260023874 1101 20-21 0.3223224168546963 1101 22-23 0.40438703762017525 1101 24-25 0.36953766066112337 1101 26-27 0.3408424761314919 1101 28-29 0.3248489891907269 1101 30-31 0.3389995580721106 1101 32-33 0.3456980263633227 1101 34-35 0.36467219285518127 1101 36-37 0.3569293540356355 1101 38-39 0.3372211691045166 1101 40-41 0.46329454185741525 1101 42-43 0.4951199946924092 1101 44-45 0.5639414799812172 1101 46-47 0.6177603590650023 1101 48-49 0.7585396765495531 1101 50-51 0.6313148012489975 1101 52-53 0.7985922447361204 1101 54-55 0.8182780226205253 1101 56-57 0.6125047115418312 1101 58-59 0.48948489205946544 1101 60-61 0.638943858128826 1101 62-63 0.7256719631271764 1101 64-65 0.7487243076034034 1101 66-67 0.5105147028092745 1101 68-69 0.67735147111852 1101 70-71 0.6045416182721297 1101 72-73 0.6365569948889558 1101 74-75 0.5671126231521342 1101 76-77 0.4103650877415035 1101 78-79 0.3927400986535474 1101 80-81 0.4111095945872094 1101 82-83 0.4729832756226173 1101 84-85 0.652434377865525 1101 86-87 0.47758245190879123 1101 88-89 0.7438169058804398 1101 90-91 0.7738129543609595 1101 92-93 0.9609548375866126 1101 94-95 0.9032189287570098 1101 96-97 0.8394140090559574 1101 98-99 0.8054695837252304 1101 100-101 1.0311397825790607 1102 1 -0.018423079306430168 1102 2 0.05863991744845265 1102 3 0.1115453211934394 1102 4 0.07545029911509715 1102 5 0.0660416920052711 1102 6 0.1338053039581908 1102 7 0.05727827842505917 1102 8 0.07270725167879988 1102 9 0.1646524204777009 1102 10-11 0.12475664241365791 1102 12-13 0.16372010307410534 1102 14-15 0.18798530749025133 1102 16-17 0.20804992836146852 1102 18-19 0.3319467733331791 1102 20-21 0.21818172603751407 1102 22-23 0.20661815247555637 1102 24-25 0.11587120460945499 1102 26-27 0.2918327176366873 1102 28-29 0.24356915206632834 1102 30-31 0.3357964550111774 1102 32-33 0.4559732299578201 1102 34-35 0.32900945766844103 1102 36-37 0.35744255257537816 1102 38-39 0.2831271646114004 1102 40-41 0.3044515202342737 1102 42-43 0.22490060407179868 1102 44-45 0.45281749795369564 1102 46-47 0.5260894800984204 1102 48-49 0.5006205527079359 1102 50-51 0.3312007181262828 1102 52-53 0.20213689511522404 1102 54-55 0.261914290522796 1102 56-57 0.38579697495099197 1102 58-59 0.19190520938606426 1102 60-61 0.28199847761773356 1102 62-63 0.5146273127480683 1102 64-65 0.4716114881706588 1102 66-67 0.41720980250038053 1102 68-69 0.3108262675240283 1102 70-71 0.2787142523636774 1102 72-73 0.21035824453792884 1102 74-75 0.3531341058817006 1102 76-77 0.09169968476481216 1102 78-79 0.15613064906074214 1102 80-81 0.19169510427550307 1102 82-83 0.3883704561898682 1102 84-85 0.3605851782025127 1102 86-87 0.3792919436246045 1102 88-89 0.5483693490143899 1102 90-91 0.644836543240487 1102 92-93 0.6418555677185971 1102 94-95 0.5361169910990462 1102 96-97 0.4413671121168967 1102 98-99 0.5011877808608709 1102 100-101 0.6222669238315177 1103 1 0.08407489311854732 1103 2 0.12483213156037465 1103 3 0.17567954665978647 1103 4 0.08376741184827097 1103 5 0.08340584447891786 1103 6 0.21542939154943497 1103 7 0.11063999780867562 1103 8 0.13897854121651676 1103 9 0.09510659728223914 1103 10-11 0.17416560429525418 1103 12-13 0.1917188865290953 1103 14-15 0.26882776491117966 1103 16-17 0.35816043120007635 1103 18-19 0.21508748703958958 1103 20-21 0.28897248029542055 1103 22-23 0.28327265369210153 1103 24-25 0.15630611945129402 1103 26-27 0.26477168762857417 1103 28-29 0.3526019987816511 1103 30-31 0.33267905841750434 1103 32-33 0.24099350570801903 1103 34-35 0.16902162311856017 1103 36-37 0.3934462022104128 1103 38-39 0.4552267185448997 1103 40-41 0.30519766784173896 1103 42-43 0.30676698687796033 1103 44-45 0.17140022301452262 1103 46-47 0.3191308426288302 1103 48-49 0.24948511069658963 1103 50-51 0.23067861755856 1103 52-53 0.23219163964076728 1103 54-55 0.3538212248293675 1103 56-57 0.32120350374255224 1103 58-59 0.2744123058986361 1103 60-61 0.1945065879178145 1103 62-63 0.2292177425939812 1103 64-65 0.2998424289654693 1103 66-67 0.2777846200186218 1103 68-69 0.2917883518711406 1103 70-71 0.011480878478849377 1103 72-73 0.06611148865796679 1103 74-75 0.1524508130998683 1103 76-77 0.04784526465126504 1103 78-79 0.05567343089366261 1103 80-81 0.19595706696812698 1103 82-83 0.21388241888248416 1103 84-85 0.095033677796998 1103 86-87 -0.02467196554003337 1103 88-89 0.1167219443104166 1103 90-91 0.19520556189417348 1103 92-93 0.37771323195217477 1103 94-95 0.4568388078062675 1103 96-97 0.09390969119232295 1103 98-99 0.055743741931429724 1103 100-101 0.29252341207158494 1104 1 0.060832584123986067 1104 2 0.0841900792607504 1104 3 0.08940130824846193 1104 4 0.07339528293386621 1104 5 0.1637115949178991 1104 6 0.11407229424945342 1104 7 0.1690420324703652 1104 8 0.19167974358818896 1104 9 0.19863300300197295 1104 10-11 0.18099046118388884 1104 12-13 0.20746605776665916 1104 14-15 0.2253599029595108 1104 16-17 0.20941319696988359 1104 18-19 0.16240793838172607 1104 20-21 0.22402314998572592 1104 22-23 0.3015857900160128 1104 24-25 0.3011665120528164 1104 26-27 0.3339484134295674 1104 28-29 0.3647261099951251 1104 30-31 0.19916619611149855 1104 32-33 0.16314151474746552 1104 34-35 0.20006123760371253 1104 36-37 0.22686811891841785 1104 38-39 0.20589010959521659 1104 40-41 0.2936424587456017 1104 42-43 0.1936263322271401 1104 44-45 0.06984824229026998 1104 46-47 0.21455226326993682 1104 48-49 0.03476294752994846 1104 50-51 0.021269488352821497 1104 52-53 0.1056886750505015 1104 54-55 0.011351992788171117 1104 56-57 0.007985486277846121 1104 58-59 -0.034207250160861236 1104 60-61 -0.08388339940491818 1104 62-63 0.07148815417202314 1104 64-65 -0.20803657008176657 1104 66-67 -0.11198518131838853 1104 68-69 -0.003765156424201166 1104 70-71 0.0989933785772763 1104 72-73 -0.01903091403811885 1104 74-75 0.021248992613088546 1104 76-77 0.01267864916610506 1104 78-79 0.06291462964325945 1104 80-81 0.12976953793246082 1104 82-83 0.0461576730183495 1104 84-85 0.19788291283034454 1104 86-87 0.08515763941748489 1104 88-89 0.18582485063251042 1104 90-91 0.47465854971297716 1104 92-93 0.49804488810694636 1104 94-95 0.4863597095456491 1104 96-97 0.43636711211689416 1104 98-99 0.15054862228481625 1104 100-101 0.39246514389624565 1105 1 0.08635619640067915 1105 2 0.013930691966031361 1105 3 0.04194513615511397 1105 4 0.0467706797653733 1105 5 0.014471509610352484 1105 6 0.04578455323125752 1105 7 0.10302107514723957 1105 8 0.029510714538965033 1105 9 0.004396010221284996 1105 10-11 0.09716181026985993 1105 12-13 0.10580244513067072 1105 14-15 0.08092510394808983 1105 16-17 0.15941755987085315 1105 18-19 0.14777746326181074 1105 20-21 0.1550136054982545 1105 22-23 -0.027649624421087537 1105 24-25 0.10286400133163198 1105 26-27 0.17134533989415246 1105 28-29 0.17459281053844222 1105 30-31 0.29630233658664196 1105 32-33 0.26681911946349857 1105 34-35 0.22022938893991295 1105 36-37 0.18146251028089466 1105 38-39 0.159550968470775 1105 40-41 0.10895059504263571 1105 42-43 0.10651010711171693 1105 44-45 0.09024480432430693 1105 46-47 0.15504004843042907 1105 48-49 0.07631444644307095 1105 50-51 0.06408302053178261 1105 52-53 0.1281698055038092 1105 54-55 0.03299775602636856 1105 56-57 0.1467786693734041 1105 58-59 0.1728512178449506 1105 60-61 0.1592116682072131 1105 62-63 0.21290645628494786 1105 64-65 0.08462265655553125 1105 66-67 0.16341684717392013 1105 68-69 0.23561182131731329 1105 70-71 0.07931926161559488 1105 72-73 0.14644237089267875 1105 74-75 0.08329072761048906 1105 76-77 0.11801543945685466 1105 78-79 0.15297805852412694 1105 80-81 0.15587829222157978 1105 82-83 0.022997405700841966 1105 84-85 -0.06822132457306651 1105 86-87 -0.003397059811820924 1105 88-89 0.12280531253540516 1105 90-91 0.1597334504569794 1105 92-93 0.3006415841077086 1105 94-95 0.08468955255820632 1105 96-97 -0.07427259182177792 1105 98-99 -0.0041274451502353315 1105 100-101 0.42067129866630637 1106 1 0.014493587360234983 1106 2 0.10765033411512093 1106 3 0.04649323786011195 1106 4 -0.014549700884906258 1106 5 0.09619794200527565 1106 6 0.057607387291525924 1106 7 0.09910119509172688 1106 8 0.09093641834547128 1106 9 0.12272533714436662 1106 10-11 0.24368893408032477 1106 12-13 0.14713156140744132 1106 14-15 0.22061551582358163 1106 16-17 0.20958638669480223 1106 18-19 0.2338998983331848 1106 20-21 0.19404110103751293 1106 22-23 0.2062796108088918 1106 24-25 0.2282409962761207 1106 26-27 0.06935875930335555 1106 28-29 0.11830873539965836 1106 30-31 0.2509006216778431 1106 32-33 0.27795239662448523 1106 34-35 0.2147646660017699 1106 36-37 0.19778109424204615 1106 38-39 0.2933875812780684 1106 40-41 0.14419110356761422 1106 42-43 0.1954214374051304 1106 44-45 0.18029145628702992 1106 46-47 0.26674052176508667 1106 48-49 0.21741742770793593 1106 50-51 0.30164342645961284 1106 52-53 0.21205877011522745 1106 54-55 0.25285179052280427 1106 56-57 0.2899636416176534 1106 58-59 0.25115000105272856 1106 60-61 0.2079880609510667 1106 62-63 0.09618981274807226 1106 64-65 0.14744482150398852 1106 66-67 0.0565587608337097 1106 68-69 0.1361127258573589 1106 70-71 -9.472059696520319E-4 1106 72-73 0.04994157787126596 1106 74-75 0.014227855881699725 1106 76-77 0.04188197643148328 1106 78-79 -0.14584851760592699 1106 80-81 -0.10572677072449466 1106 82-83 -0.20876496047679893 1106 84-85 0.1051164282025141 1106 86-87 0.3119742352912702 1106 88-89 0.27500997401439164 1106 90-91 0.416555293240485 1106 92-93 0.46008473438526565 1106 94-95 0.5068201160990462 1106 96-97 0.42087232045022915 1106 98-99 0.4943909058608682 1106 100-101 0.5703398404981854 1107 1 0.010311412008704224 1107 2 0.04614612092239412 1107 3 0.04674590018434088 1107 4 0.08174889299433374 1107 5 0.16110372674472728 1107 6 0.14778586640649394 1107 7 0.04572740993870639 1107 8 0.032188227692039106 1107 9 0.17313050153642706 1107 10-11 0.050522771445919545 1107 12-13 0.010818945092303522 1107 14-15 -0.06879819126905318 1107 16-17 0.035011260040541003 1107 18-19 0.10912832833731301 1107 20-21 -0.04417972971104689 1107 22-23 -0.04351005265264263 1107 24-25 0.030268433724423005 1107 26-27 0.10222457040757149 1107 28-29 0.135577009799988 1107 30-31 -9.229411840223634E-4 1107 32-33 0.12668456163689257 1107 34-35 0.07479523103485519 1107 36-37 0.05814768078050747 1107 38-39 0.047815957001809295 1107 40-41 -0.028335907391856097 1107 42-43 -0.16160787400926324 1107 44-45 -0.16512088087177546 1107 46-47 -0.1559948871472443 1107 48-49 -0.12382113463697664 1107 50-51 -0.21048145722524936 1107 52-53 -0.1781794820559881 1107 54-55 -0.19883736373692074 1107 56-57 -0.15017800437076545 1107 58-59 -0.18660492295471443 1107 60-61 -0.21143204347407618 1107 62-63 -0.2868036219086676 1107 64-65 -0.15887755235871026 1107 66-67 -0.13657121486934898 1107 68-69 -0.05566153231055182 1107 70-71 0.2047616055481285 1107 72-73 0.035828674645458136 1107 74-75 0.060079928875914845 1107 76-77 -0.24890618785718743 1107 78-79 -0.2161222458937715 1107 80-81 -0.036084052052039794 1107 82-83 0.012445104659683182 1107 84-85 -0.12620142229376796 1107 86-87 -0.06312947903875354 1107 88-89 0.06023762858428228 1107 90-91 -0.2206183781821771 1107 92-93 6.748398443221504E-4 1107 94-95 -0.1721129510018642 1107 96-97 -0.2673136157573772 1107 98-99 -0.11458765338230847 1107 100-101 -0.2411604955233173 1108 1 -0.2219507325201846 1108 2 -0.0912928179775605 1108 3 -0.12730744412793626 1108 4 -0.055767937058298855 1108 5 -0.11612572204554539 1108 6 -0.15580231936019118 1108 7 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-0.906846647874282 2316 84-85 -0.7744749639516684 2316 86-87 -0.8105955176474922 2316 88-89 -0.7761939369342166 2316 90-91 -0.821885709356561 2316 92-93 -0.8855387278892017 2316 94-95 -0.729738667730917 2316 96-97 -0.767888400913904 2316 98-99 -1.0756424542402776 2316 100-101 -1.0764074428533732 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 14.0 5 28.0 6 55.0 7 104.0 8 234.0 9 458.0 10 815.0 11 1369.0 12 2193.0 13 3378.0 14 4104.0 15 5478.0 16 7405.0 17 10323.0 18 9640.0 19 5464.0 20 4203.0 21 4201.0 22 4395.0 23 5003.0 24 5896.0 25 7074.0 26 8932.0 27 11129.0 28 13836.0 29 17445.0 30 21938.0 31 27556.0 32 34568.0 33 44595.0 34 60853.0 35 93312.0 36 136912.0 37 276468.0 38 443303.0 39 113292.0 40 207.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.5053571932373 19.90028552888674 13.493934590952922 23.100422686923036 2 14.715113837575275 21.37616691271226 41.31459057580441 22.594128673908056 3 18.986602105919253 26.407024155453662 29.499389693172933 25.10698404545415 4 11.246165743751929 17.17256245184636 39.79229368599353 31.788978118408178 5 11.699639587126711 39.65299079201378 35.59841810778913 13.048951513070378 6 29.966786611916994 38.25649911844569 18.530459071929638 13.24625519770767 7 27.43343615728891 33.23964714742878 21.966140234946486 17.360776460335828 8 25.15365881107036 36.57584685482777 20.720205340112596 17.55028899398927 9 26.502970737014024 15.978784984432126 21.22309528000531 36.29514899854854 10-11 23.788733598401652 27.748382519341774 28.642456067324417 19.820427814932152 12-13 24.784553033210504 25.3238738179814 28.985173728238884 20.906399420569215 14-15 21.60684553610333 27.199918048634096 27.318228119129586 23.87500829613299 16-17 19.959478669310375 30.27452714593703 28.623879883146785 21.14211430160581 18-19 19.6923505325396 29.141321379206858 31.11753239820284 20.0487956900507 20-21 20.982940264956845 28.32072561586163 30.94760595243935 19.748728166742175 22-23 20.178734903732327 28.674377987962664 30.62353780550532 20.523349302799694 24-25 19.840522942772292 28.613853207666818 31.25536781407281 20.29025603548808 26-27 19.83786113771343 28.928242281270343 30.96816198736435 20.265734593651878 28-29 19.628131890646376 28.844649278385994 31.151375721550885 20.375843109416746 30-31 20.195826645264848 28.817717820621496 31.16161379335221 19.82484174076145 32-33 19.57109847356275 29.163868186346686 31.093755523316418 20.171277816774143 34-35 19.89083538846608 29.16282963734076 31.102283388647876 19.844051585545284 36-37 19.961217381769284 28.78527299327364 30.71333037077222 20.54017925418486 38-39 19.8378718022893 29.195519796391107 31.012734934031265 19.953873467288325 40-41 20.461520981259614 28.63810731309663 31.100346095647925 19.800025609995835 42-43 19.874544149421595 28.89957399839122 30.87162598429457 20.354255867892608 44-45 20.027825692991055 29.164926208166776 30.57597813767694 20.23126996116523 46-47 20.07728693423684 29.003992773469626 30.135483014479036 20.783237277814496 48-49 20.149156812706575 29.242650204799308 30.120109005036827 20.488083977457286 50-51 20.463948038512275 29.34845774503852 29.561645288392967 20.625948928056236 52-53 20.110556748171366 29.50506664319512 30.01867502888551 20.365701579748 54-55 20.382488628862667 29.263626427929896 30.002957758163596 20.350927185043844 56-57 20.422510825009272 29.542391760728698 29.41235457839096 20.622742835871072 58-59 20.227180419098133 29.464739818506718 29.801243284539307 20.506836477855842 60-61 20.410836906426734 29.615693019004517 29.62388104493639 20.34958902963236 62-63 20.42273486713202 29.489542544012785 29.94676768230572 20.14095490654947 64-65 20.35908769390977 29.085987781524686 29.861753113503696 20.693171411061844 66-67 20.256054734278468 29.695250572072474 29.49549411768101 20.55320057596805 68-69 20.448669300268072 29.392261367145007 29.71584662397885 20.443222708608065 70-71 20.488087457238784 29.002962084453316 29.82135874983588 20.687591708472024 72-73 20.398610411238774 29.22886913575616 29.587376110572656 20.785144342432417 74-75 20.378801432417536 29.402685638600246 29.36466770741054 20.853845221571675 76-77 20.056470527004038 29.084128579602154 29.985907668664314 20.873493224729494 78-79 19.909285932747665 29.090356945158106 29.82200587066836 21.17835125142587 80-81 20.109307672385746 29.626873585590317 29.208060430867878 21.055758311156062 82-83 20.538628238016777 29.333935945196465 29.43825080003578 20.689185016750976 84-85 20.26778629038076 29.14174205370154 29.48877490657779 21.10169674933991 86-87 20.039248741245054 29.470972616166236 29.525649276310705 20.96412936627801 88-89 20.4734283005498 29.047508643526072 29.637107966339503 20.84195508958463 90-91 20.297271686899776 29.18817787673728 29.66341747745642 20.85113295890652 92-93 20.221496217294053 29.238853651804387 29.722377275040866 20.81727285586069 94-95 19.934805317727587 29.202773713781134 29.702537088177667 21.159883880313615 96-97 20.266213226687405 29.12225240586389 29.481630522647528 21.129903844801174 98-99 20.20867841333418 28.949025792265203 29.532764083592568 21.30953171080805 100-101 20.00855590591966 29.101153748763686 29.488658374796294 21.40163197052036 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1034.0 1 687.5 2 828.5 3 1222.0 4 858.5 5 1065.5 6 2589.5 7 4204.5 8 4636.5 9 4034.5 10 2941.5 11 2089.0 12 1827.0 13 1799.5 14 1688.5 15 1571.0 16 1612.5 17 1769.5 18 1976.5 19 2174.5 20 2497.5 21 3007.0 22 3653.5 23 4523.5 24 5390.5 25 6616.5 26 8632.0 27 10690.5 28 12637.0 29 15711.0 30 20129.0 31 25045.0 32 30625.5 33 37168.0 34 43638.0 35 48621.0 36 53825.5 37 57993.5 38 57563.0 39 56786.0 40 56134.5 41 54030.0 42 51597.5 43 51969.0 44 53750.5 45 54024.0 46 51691.5 47 49095.5 48 51394.5 49 50909.0 50 47623.5 51 43534.0 52 37843.0 53 33690.0 54 29935.0 55 25869.5 56 21820.0 57 18131.0 58 14690.0 59 11428.0 60 8633.5 61 6504.5 62 4724.0 63 3311.0 64 2394.5 65 1706.5 66 1228.0 67 997.0 68 758.0 69 535.0 70 378.0 71 272.0 72 216.5 73 185.5 74 144.5 75 84.0 76 45.0 77 23.0 78 11.5 79 5.0 80 8.5 81 8.5 82 2.0 83 1.0 84 2.0 85 1.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2770908419348602 2 0.01918931631207311 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.2463129099371803E-4 12-13 0.0 14-15 0.0 16-17 3.2463129099371803E-4 18-19 0.0 20-21 0.0 22-23 7.21402868874929E-5 24-25 0.002344559323843519 26-27 0.0022002787500685333 28-29 0.0017674370287435758 30-31 9.378237295374075E-4 32-33 0.001370665450862365 34-35 0.0017674370287435758 36-37 0.0 38-39 7.21402868874929E-5 40-41 7.574730123186753E-4 42-43 0.0031020323361621942 44-45 0.0042562769263620815 46-47 0.013273812787298691 48-49 0.014211636516836101 50-51 0.00609585424199315 52-53 0.00811578227484295 54-55 2.1642086066247867E-4 56-57 0.0033184531968246727 58-59 0.0015149460246373506 60-61 0.0012624550205311256 62-63 0.0 64-65 6.131924385436896E-4 66-67 0.0 68-69 0.0 70-71 1.442805737749858E-4 72-73 0.00162315645496859 74-75 0.0 76-77 0.20282241658418626 78-79 0.10875148248289553 80-81 6.492625819874361E-4 82-83 0.0 84-85 5.771222950999432E-4 86-87 0.12155638340542553 88-89 0.5867530233994235 90-91 0.306848710275951 92-93 0.29162710974269 94-95 0.0023084891803997728 96-97 0.0031381024796059407 98-99 0.0025970503279497442 100-101 7.935431557624219E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1386188.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.865369901794516 #Duplication Level Percentage of deduplicated Percentage of total 1 69.89706542705514 33.45648891715927 2 15.093124719548069 14.448759953501739 3 5.951836075434901 8.546605060366096 4 3.025309607410195 5.792302537045669 5 1.7202793344812641 4.117090333967929 6 1.087965194734258 3.1245513891739893 7 0.7222493130991141 2.4199511372964273 8 0.4664456142465423 1.7861273491984402 9 0.3718528966963366 1.6018988804481542 >10 1.4820280624396251 12.210431141203957 >50 0.1086524347418915 3.6362216746532705 >100 0.06852276735683495 5.976335091005282 >500 0.003162567995847096 0.9755918612035442 >1k 0.001204787807941751 1.0004610197756754 >5k 3.0119695198543774E-4 0.9071836540007835 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7275 0.5248205871065108 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5310 0.38306492337258724 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4046 0.29187960074679625 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1783 0.1286261315203998 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1771 0.12776044807774992 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1494 0.10777758860991439 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.21402868874929E-5 0.0 5 0.0 0.0 0.0 7.21402868874929E-5 0.0 6 0.0 0.0 0.0 7.21402868874929E-5 0.0 7 0.0 0.0 0.0 7.21402868874929E-5 0.0 8 0.0 0.0 0.0 7.21402868874929E-5 0.0 9 0.0 0.0 0.0 7.21402868874929E-5 0.0 10-11 0.0 0.0 0.0 1.8035071721873223E-4 0.0 12-13 0.0 0.0 0.0 2.885611475499716E-4 0.0 14-15 0.0 0.0 0.0 3.967715778812109E-4 0.0 16-17 0.0 0.0 0.0 0.0011181744467561399 0.0 18-19 0.0 0.0 0.0 0.0016592265984123366 0.0 20-21 0.0 0.0 0.0 0.003426663627155912 0.0 22-23 0.0 0.0 0.0 0.007574730123186754 0.0 24-25 0.0 0.0 0.0 0.012335989057761284 0.0 26-27 0.0 0.0 0.0 0.014283776803723593 0.0 28-29 0.0 0.0 0.0 0.016159424262798407 0.0 30-31 0.0 0.0 0.0 0.01958608788995432 0.0 32-33 0.0 0.0 0.0 0.025501591414728738 0.0 34-35 0.0 0.0 0.0 0.03289597082069676 0.0 36-37 0.0 0.0 0.0 0.04537624045223303 0.0 38-39 0.0 0.0 0.0 0.06557552078073103 0.0 40-41 0.0 0.0 0.0 0.09093283162168479 0.0 42-43 0.0 0.0 0.0 0.11542445901998863 0.0 44-45 0.0 0.0 0.0 0.14384773205366083 0.0 46-47 0.0 0.0 0.0 0.1753369672800515 0.0 48-49 0.0 0.0 0.0 0.21422058191241017 0.0 50-51 0.0 0.0 0.0 0.2580458061965621 0.0 52-53 0.0 0.0 0.0 0.2971097715461395 0.0 54-55 0.0 0.0 0.0 0.34266636271559126 0.0 56-57 0.0 0.0 0.0 0.395617333291011 0.0 58-59 0.0 0.0 0.0 0.4550248595428614 0.0 60-61 0.0 0.0 0.0 0.522223536778561 0.0 62-63 0.0 0.0 0.0 0.592379965776648 0.0 64-65 0.0 0.0 0.0 0.662211763483741 0.0 66-67 0.0 0.0 0.0 0.7425399729329644 0.0 68-69 0.0 0.0 0.0 0.8385947649236611 0.0 70-71 0.0 0.0 0.0 0.9478512294147692 0.0 72-73 0.0 0.0 0.0 1.058406219069852 0.0 74-75 0.0 0.0 0.0 1.1706565054667908 0.0 76-77 0.0 0.0 0.0 1.2935114140361914 0.0 78-79 0.0 0.0 0.0 1.4351588673397835 0.0 80-81 0.0 0.0 0.0 1.5963563383898864 0.0 82-83 0.0 0.0 0.0 1.7618100863663515 0.0 84-85 0.0 0.0 0.0 1.9293559026625537 0.0 86-87 0.0 0.0 0.0 2.1086605857214176 0.0 88-89 0.0 0.0 0.0 2.312132264887591 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7330 0.0 31.160854 1 GGTATCA 4850 0.0 30.905867 1 GTACATG 7570 0.0 28.538563 1 CGTATAC 85 6.4545544E-4 27.906494 3 TACATGG 7570 0.0 27.20262 2 ACATGGG 7380 0.0 27.127535 3 TAACGCA 90 9.013601E-4 26.356134 4 TCAACGC 8645 0.0 26.066507 4 ATCAACG 8675 0.0 25.976364 3 CATGGGG 2740 0.0 25.798231 4 CAACGCA 8800 0.0 25.607382 5 GTGTAGC 915 0.0 25.482798 1 AACGCAG 8935 0.0 25.220474 6 GTCCTAC 705 0.0 24.973804 1 TATAACG 95 0.0012345564 24.972572 2 TATCAAC 9105 0.0 24.805265 2 GGCCACG 160 1.8802202E-6 23.72052 6 GTATAGG 710 0.0 23.457506 1 CAGTCCG 225 2.8558134E-9 23.193398 9 ATAGCCC 880 0.0 22.103212 3 >>END_MODULE